Papers by Narangerel Altangerel
Small Methods, 2024
Amino-acid protein composition plays an important role in biology, medicine, and nutrition. Here,... more Amino-acid protein composition plays an important role in biology, medicine, and nutrition. Here, a groundbreaking protein analysis technique that quickly estimates amino acid composition and secondary structure across various protein sizes, while maintaining their natural states is introduced and validated. This method combines multivariate statistics and the thermostable Raman interaction profiling (TRIP) technique, eliminating the need for complex preparations. In order to validate the approach, the Raman spectra are constructed of seven proteins of varying sizes by utilizing their amino acid frequencies and the Raman spectra of individual amino acids. These constructed spectra exhibit a close resemblance to the actual measured Raman spectra. Specific vibrational modes tied to free amino and carboxyl termini of the amino acids disappear as signals linked to secondary structures emerged under TRIP conditions. Furthermore, the technique is used inversely to successfully estimate amino acid compositions and secondary structures of unknown proteins across a range of sizes, achieving impressive accuracy ranging between 1.47% and 5.77% of root mean square errors (RMSE). These results extend the uses for TRIP beyond interaction profiling, to probe amino acid composition and structure.
PNAS, 2023
Significance Proteins are the machinery of life, and their function is controlled by interactions... more Significance Proteins are the machinery of life, and their function is controlled by interactions: with cofactors, substrates, and proteins that sterically or conformationally modulate activity. We present a thermostable Raman interaction profiling (TRIP) technique that facilitates low-concentration and low-dose screening of binding between protein and ligand in physiologically relevant conditions. The proposed method can be used over a large variety of molecules ranging from small molecules to large proteins such as antibodies and offers an essential tool for researchers and pharmaceutical companies in the field of drug development. The TRIP holds the promise for future developments of highthroughput drug screening and real-time binding measurements between protein and drug.
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Papers by Narangerel Altangerel