Papers by SANTOSHREDDY MACHENAHALLI
Journal of Mycopathological Research, 2013
Journal of Mycopathological Research, 2013
Karnataka Journal of Agricultural Sciences, 2015
Chilli (Capsicum annuum L.), is severely infected by fruit rot disease which reduce quality and q... more Chilli (Capsicum annuum L.), is severely infected by fruit rot disease which reduce quality and quantity of fruits and seeds. Fruit rot causing fungal pathogens are carried along with seed to cause deterioration of seed in storage, pre and post emergence damping off in nursery. In the present study, seed borne nature of fungi was tested by standard blotter method which revealed the presence of Colletotrichum capsici, C. gloeosporioides and C. acutatum also Alternaria alternata. Fusarium sporotrichioides and F. oxysporum. The frequency of these pathogens varied from 72.85 % (C. capsici) to 3.45% (F. sporotrichioides). As a preventive measure, seed treatment with eleven chemical fungicides and four bio fungicides in different combinations tested in nursery which revealed that carboxin 37.5% + thiram 37.5% WS (Vitavax power) @ 0.2% and metalaxyl 4%+ mancozeb 64% WP (Ridomil gold) @ 0.2% showed least infection (7.25% and 10.09%) with highest seedling vigour index (932.02 and 871.70). Am...
Journal of Mycopathological Research, 2016
BIOINFOLET - A Quarterly Journal of Life Sciences, 2013
Pseudoperonospora cubensis (Berk. and Curt.) Rostow is a causal agent of downy mildew of gherkin ... more Pseudoperonospora cubensis (Berk. and Curt.) Rostow is a causal agent of downy mildew of gherkin or pickling cucumber (Cucumis sativus L.). The efficacy of fungicides, botanicals and bioagents against spoarangial germination of P. cubensis, was studied under in vitro conditions during 2010. Among fungicides Fenamidon 10% + Mancozeb 50%WP (Sectin 60 WP) (98.41%) was superior in inhibiting the sporangial germination at 0.2% concentration which was at par with Metalaxyl 8% + Mancozeb 64% WP (Ridomil-MZ 72 WP) (97.73%). Among botanicals Garlic (Allium sativum) bulb extract (82.26%) was found to be superior in inhibiting the sporangial germination at 10 per cent concentration followed by Pongamia leaf extract (76.30%). Among organics maximum inhibition of sporangial germination was observed in panchagavya (38.07%) at 10 per cent concentration. Among bioagents Pseudomonas fluorescens (10.24%) was found to be superior in inhibiting sporangial germination at 6% concentration.
Journal of Mycopathological Research, 2012
Journal of Pure and Applied Microbiology, 2016
Twister disease is a major disease of onion (Allium ascalonicum L.). The causal organisms of the ... more Twister disease is a major disease of onion (Allium ascalonicum L.). The causal organisms of the anthracnose/twister disease of onion were characterized and identified using molecular markers to assess the diversity of the pathogens and the identity of the pathogen was confirmed by sequencing the amplified ITS rDNA region, and analysing in NCBI BLAST program and identified as C. gloeosporioides, C. acutatum, G. acutata , F. oxysporum and G. moniliformis. The phylogenetic tree showed that all five isolates of Colletotrichum spp. Framed into 3 clusters. Since, there is no gene sequences of C. gloeosporioides C. acutatum and F. oxysporum causing onion twister disease in the Genbank we have deposited our sequences Genbank. Specific DNA amplification of region CgInt was fairly consistent band of 450 bp for C. gloeosporioides and for C. acutatum it was 490 bp which was positive for all isolates of C. acutatum. For F. oxysporum amplification was at 550-570 bp. The products of the PCR ITS rDNA digestion with DraI revealed that no restriction sites were present. The digestion with HaeIII resulted in a characteristic pattern of three fragments in 19 C. gloeosporioides isolates. In 24 isolates of C. acutatum isolates and 25 isolates F. oxysporum showed four clusters.
Vegetos- An International Journal of Plant Research, 2016
Integrated Management of DieBack and Fruit Rot of Chilli (Capsicum annuum L.) Fruit rot and die-b... more Integrated Management of DieBack and Fruit Rot of Chilli (Capsicum annuum L.) Fruit rot and die-back diseases are major yield limiting factor in all chilli growing areas of India. Integrated management of chilli fruit rot disease during kharif 2012 and 2013 at Main Agricultural Research Station, University of Agricultural Sciences Dharwad, Karnataka, indicated that adoptive module including seed treatment with carboxin + thiram at 2 g/kg, seedling dip in P. fluorescens (10 g/l), spray with neem oil (10 ml/l), hexaconazole, propicoanzole (0.1%) and carbendazim + mancozeb (0.2%) showed least seedling infection (7.06%), die-back incidence (1.21%) and severity (9.30 PDI), fruit rot incidence (4.47%), severity (2.68 PDI) with high dry chilli yield (8.92 q/ha) and C:B ratio (2.44)...
Vegetos- An International Journal of Plant Research, 2014
Chilli (Capsicum annuum L.), an important economic crop worldwide, is severely infected by fruit ... more Chilli (Capsicum annuum L.), an important economic crop worldwide, is severely infected by fruit rot which may cause yield losses of up to 50%. The disease has been reported in all the chilli producing regions of the world and has become a serious constraint in chilli production. Different species of Colletotrichum, (C. capsici, C. gloeosporioides, C. acutatum), Alternaria alternata and Fusarium spp. are known to cause fruit rot in chilli. In the present study detection of pathogens from different plant parts was carried out by standard isolation method and PCRwas done by using specific primers. The C. capsici was amplified as single band at 450bp whereas C. gloeosporioides and C. acutatum were amplified at 450 and 490bp respectively, Alternaria alternata amplified at 390bp and Fusarium sp. amplified at 550 – 570bp.
Indian phytopathology/Indian Phytopathology, Jun 14, 2024
Journal of Mycopathological research
Black rot disease of coffee is the second important disease of coffee. A field experiment was con... more Black rot disease of coffee is the second important disease of coffee. A field experiment was conducted in 2020 and 2021 two seasons to know the efficacy of different fungicides in managing the black rot disease of coffee. A total of eight fungicides were evaluated against the black rot disease of coffee. Results indicated that, out of eight fungicides tested, tebuconazole 50%+ trifloxystrobin 25% w/w W G @ 1g/L was found significantly superior in managing the disease. In both the seasons percent disease reduction was significantly higher in the plants sprayed with tebuconazole 50%+ trifloxystrobin 25% w/w W G @ 1g/L and was on par with the fungicide pyraclostrobin 13.3% + epoxyconazole 5% w/v SE @ 1ml/L followed by tebuconazole 25.6 SC @ 1ml/L when compared to other fungicides and unsprayed control plants. From the present findings, it can be concluded that the fungicides tebuconazole 50%+ trifloxystrobin 25% w/w WG @ 1g/L and pyraclostrobin 13.3% + epoxyconazole 5% w/v SE @ 1ml/L can be used for the effective management of the disease.
Journal of Mycopathological Research, 2019
Journal of Agrometeorology, 2021
Journal of Mycopathological Research, 2019
International Journal of Current Microbiology and Applied Sciences, 2020
Coffee is one of the most popular non-alcoholic beverages consumed all over the world. The coffee... more Coffee is one of the most popular non-alcoholic beverages consumed all over the world. The coffee industry offers lively hood for millions of people directly and indirectly among the coffee growing countries. The coffee crop is known to be affected by many diseases at different seasons. Earlier, the pathogen Myrothecium was known to cause stem necrosis and leaf spot disease on coffee seedlings in the nursery. In the recent years, there was a gradual increase in the incidence of leaf spot and fruit rot of Arabica coffee caused by Myrothecium in the field condition during rainy season. During the visit to coffee plantations in Chikkamagaluru and Hassan districts, the plants and weeds growing in and around the coffee plantation were observed for the presence of leaf spot with typical symptoms caused by Myrothecium. A total of eleven different plant species viz., Colocasia sp., Alternanthera bettzickiana, Remusatia vivipara, Phlebodium aureum, Alternanthera brasiliana, Polypodium triseriale, Urtica sp., Artocarpus heterophyllus, Tarenna asiatica, Impatiens dasysperma and Hibiscus rosa-sinensis with typical symptoms of Myrothecium were identified. Based on the morphological character the fungal cultures were identified as Myrothecium roridum. The development of typical symptoms of Myrothecium on the Arabica coffee leaves cross-inoculated with pure fungal culture indicated the cross infecting ability of the fungus isolated from different host plants. As per our knowledge this is the first report indicating the cross infecting ability of the Myrothecium roridum cultures on Arabica coffee leaves isolated from different host plants, majority of them were weed plants commonly grown in and around coffee plantation.
International Journal of Microbiology Research, 2018
Degradation of coffee pulp waste is time taken process. The microorganisms isolated from coffee p... more Degradation of coffee pulp waste is time taken process. The microorganisms isolated from coffee pulp waste could be used as catalysts in the biodegradation of pulp waste which reduces the composting period. An experiment was conducted in which coffee pulp waste was collected from Central Coffee Research Institute farm and isolated two Aspergillus spp. (Asp.1 and Asp.2) two Trichoderma spp. (Tri.1 and Tri.2) and two-gram negative bacteria (CPB-1 and CPB-2). These isolated microbes were subjected to pectinolytic enzyme activity assay by plate assay method. Clear zone of pectinolytic enzyme activity was observed in Aspergillus spp., Trichoderma spp. and CPB-1 bacterium isolate whereas clear zone was not observed in CPB-2 bacterium isolate.
International Journal of Current Microbiology and Applied Sciences, 2019
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Papers by SANTOSHREDDY MACHENAHALLI