The physicochemical, biochemical and microbiological changes of karasumi-like Chinook salmon (Onc... more The physicochemical, biochemical and microbiological changes of karasumi-like Chinook salmon (Oncorrhynchus tshawytscha) roe were determined during 20 days of salted-drying processing. Colour parameters (L*, a*, and b*), water activity (aw) and the pH value decreased at the end of the processing. Total bacterial and LAB counts (log CFU/g) varied over the processing period. Saturated fatty acids, such as palmitic acid (C16:0) and stearic acid (C18:0), and unsaturated fatty acids such as palmitoleic acid (C16:1), oleic acid (C18:1), and arachidonic acid (C20: 4), were significantly decreased (p < 0.05), but conversely, gamma-linolenic acid (C18:3), and eicosapentaenoic acid (C22:5) were increased (p < 0.05) by salted-drying time. Cholesterol and tocopherol contents were reduced, while the astaxanthin and lutein contents were increased (P < 0.05) during the salted-drying process. Salmon karasumi-like product is a high nutrition product that contains a substantial content of functional compounds.
The effectiveness of Liquid Organic Fertilizer, Fermented from Cow’s Rumen, effected to onionâ€... more The effectiveness of Liquid Organic Fertilizer, Fermented from Cow’s Rumen, effected to onion’s Production. The objectives of this research were to determine: (1) an effectiveness of liquid organic fertilizer; (2) a dosage of liquid organic fertilizer; and (3) an efficiency of the application of liquid organic fertilizer fermented from cow rumen to increase onion’s harvest. This research used a Randomized Group Design with Factorial Pattern using 2 factors. Firstly, a dosage of liquid organic fertilizer fermented from cow rumen (P) that is using some levels: 0, 8, 16, 24, and 32 milliliter/liter clean water. Secondly, the way of fertilizer application (A): by showering to soil media, and by spraying to all of the onion leaves. Analyses of the onion components were made for onions diametric and the weight of dry and wet union. Analyses were made also for the fertilizer nutrition and for the nutrient-rich soil before and after onions were planted. All analyses were performed i...
Probiotic beverages were made from banana peels that inoculated bacteri Lactobacillus casei. The ... more Probiotic beverages were made from banana peels that inoculated bacteri Lactobacillus casei. The aims of this research was to study effect of kind of banana peels and concentration of inculant on quality of beverage. This research used completely random design, with combination of two factors that were kinds of banana peels (keprok, susu and ambon) and concentration of inoculant (4%, 5%, 6%, 7%, and 8% in milk media) in three times. Protein and calsium content were measured as well as acidity (pH). Sensoric method was used for determining organoleptic properties (flavour, color, texture, taste). Data was analyzed using ANOVA, followed LSD test in 0,05 level. Result showed probiotic beverage from”susu” peel that inoculated Lactobacillus casei concentration 8% (K2S5)
Using quality seeds could increase crop production, including kidney bean, "Inerie", cultivar. Th... more Using quality seeds could increase crop production, including kidney bean, "Inerie", cultivar. The qualified seeds providing enough food supply in the endosperm as a primary energy for a normal and strong germination (viability) and seedling growth (vigor). Seeds from harvested yield are stored for the next planting season. During storage the quality of the seeds will be deprived physically, physiologically and chemically, impact on the production. One way to overcome the drawbacks of the deteriorated seeds is by osmoconditioning using Polyethylene Glycol solution. Osmoconditioning strongly influenced by the osmotic potential and duration of osmoconditioning. The results showed that: 1) Osmoconditioning using PEG-6000 during invigoration affect the electrical conductivity and the chemical content of seeds, which has been deteriorating during 1 year storage; and 2) There is an interaction between osmotic potency and duration of osmoconditioning using PEG-6000 in relation to the electrical conductivity and chemical composition on the seed during 1 year storage.-1.0 MPa with 1 hour osmoconditioning gave the best effect on the electrical conductivity, the levels of free fatty acids and P-total of the "Inerie" seeds.
Fish is the main source of ω-3 long chain polyunsaturated fatty acids (LC-PUFA) such as eicosapen... more Fish is the main source of ω-3 long chain polyunsaturated fatty acids (LC-PUFA) such as eicosapentaenoic acid (EPA, 20:5ω-3) and docosahexaenoic acid (DHA, 22:5ω-3), which have positive effects on human health and can be beneficial in human diet context. Studies involving fatty acyl desaturase (Fads) and elongase of very long chain fatty acids (Elovl) enzymes that convert C18 PUFA to C20/22 LC-PUFA have been performed in some fish species. However, very little is known about LC-PUFA biosynthesis in tuna species. This study investigated the Δ6 Fads gene performance in the SBT cell line. The Δ6 Fads nucleotide sequences from various fish species were identified and retrieved to compare them with the SBT Δ6 Fads nucleotide sequence. The Δ6 Fads gene was performed using real time PCR (RT-PCR) and then was compared it with β-actin gene performance as a reference housekeeping gene. By performing multiple sequence alignments and comparing the highly conserved regions among fish Δ6 Fads sequences, the SBT Δ6 Fads nucleotide sequence was determined to be ≥ 75% identical to the other fish Δ6 Fads sequences. The results showed that when the SBT Δ6 Fads and β-actin cDNAs were performed in a standard PCR system and the products were analysed by electrophoresing them on a 2% (w/v) agarose gel, the target genes that were obtained were similar to the expected sizes. The observed band size for the Δ6 Fads PCR product was 207 bp and for the β-actin PCR product was 98 bp. The presence of the observed bands indicated that the primer pairs that were designed and used were successfully amplified the target genes. The results of this study might provide relevant information to support further investigating of the desaturase and elongase gene expression that might contribute to a better understanding of ω-3 LC-PUFA biosynthesis in fish.
The physicochemical, biochemical and microbiological changes of karasumi-like Chinook salmon (Onc... more The physicochemical, biochemical and microbiological changes of karasumi-like Chinook salmon (Oncorrhynchus tshawytscha) roe were determined during 20 days of salted-drying processing. Colour parameters (L*, a*, and b*), water activity (aw) and the pH value decreased at the end of the processing. Total bacterial and LAB counts (log CFU/g) varied over the processing period. Saturated fatty acids, such as palmitic acid (C16:0) and stearic acid (C18:0), and unsaturated fatty acids such as palmitoleic acid (C16:1), oleic acid (C18:1), and arachidonic acid (C20: 4), were significantly decreased (p < 0.05), but conversely, gamma-linolenic acid (C18:3), and eicosapentaenoic acid (C22:5) were increased (p < 0.05) by salted-drying time. Cholesterol and tocopherol contents were reduced, while the astaxanthin and lutein contents were increased (P < 0.05) during the salted-drying process. Salmon karasumi-like product is a high nutrition product that contains a substantial content of functional compounds.
The effectiveness of Liquid Organic Fertilizer, Fermented from Cow’s Rumen, effected to onionâ€... more The effectiveness of Liquid Organic Fertilizer, Fermented from Cow’s Rumen, effected to onion’s Production. The objectives of this research were to determine: (1) an effectiveness of liquid organic fertilizer; (2) a dosage of liquid organic fertilizer; and (3) an efficiency of the application of liquid organic fertilizer fermented from cow rumen to increase onion’s harvest. This research used a Randomized Group Design with Factorial Pattern using 2 factors. Firstly, a dosage of liquid organic fertilizer fermented from cow rumen (P) that is using some levels: 0, 8, 16, 24, and 32 milliliter/liter clean water. Secondly, the way of fertilizer application (A): by showering to soil media, and by spraying to all of the onion leaves. Analyses of the onion components were made for onions diametric and the weight of dry and wet union. Analyses were made also for the fertilizer nutrition and for the nutrient-rich soil before and after onions were planted. All analyses were performed i...
Probiotic beverages were made from banana peels that inoculated bacteri Lactobacillus casei. The ... more Probiotic beverages were made from banana peels that inoculated bacteri Lactobacillus casei. The aims of this research was to study effect of kind of banana peels and concentration of inculant on quality of beverage. This research used completely random design, with combination of two factors that were kinds of banana peels (keprok, susu and ambon) and concentration of inoculant (4%, 5%, 6%, 7%, and 8% in milk media) in three times. Protein and calsium content were measured as well as acidity (pH). Sensoric method was used for determining organoleptic properties (flavour, color, texture, taste). Data was analyzed using ANOVA, followed LSD test in 0,05 level. Result showed probiotic beverage from”susu” peel that inoculated Lactobacillus casei concentration 8% (K2S5)
Using quality seeds could increase crop production, including kidney bean, "Inerie", cultivar. Th... more Using quality seeds could increase crop production, including kidney bean, "Inerie", cultivar. The qualified seeds providing enough food supply in the endosperm as a primary energy for a normal and strong germination (viability) and seedling growth (vigor). Seeds from harvested yield are stored for the next planting season. During storage the quality of the seeds will be deprived physically, physiologically and chemically, impact on the production. One way to overcome the drawbacks of the deteriorated seeds is by osmoconditioning using Polyethylene Glycol solution. Osmoconditioning strongly influenced by the osmotic potential and duration of osmoconditioning. The results showed that: 1) Osmoconditioning using PEG-6000 during invigoration affect the electrical conductivity and the chemical content of seeds, which has been deteriorating during 1 year storage; and 2) There is an interaction between osmotic potency and duration of osmoconditioning using PEG-6000 in relation to the electrical conductivity and chemical composition on the seed during 1 year storage.-1.0 MPa with 1 hour osmoconditioning gave the best effect on the electrical conductivity, the levels of free fatty acids and P-total of the "Inerie" seeds.
Fish is the main source of ω-3 long chain polyunsaturated fatty acids (LC-PUFA) such as eicosapen... more Fish is the main source of ω-3 long chain polyunsaturated fatty acids (LC-PUFA) such as eicosapentaenoic acid (EPA, 20:5ω-3) and docosahexaenoic acid (DHA, 22:5ω-3), which have positive effects on human health and can be beneficial in human diet context. Studies involving fatty acyl desaturase (Fads) and elongase of very long chain fatty acids (Elovl) enzymes that convert C18 PUFA to C20/22 LC-PUFA have been performed in some fish species. However, very little is known about LC-PUFA biosynthesis in tuna species. This study investigated the Δ6 Fads gene performance in the SBT cell line. The Δ6 Fads nucleotide sequences from various fish species were identified and retrieved to compare them with the SBT Δ6 Fads nucleotide sequence. The Δ6 Fads gene was performed using real time PCR (RT-PCR) and then was compared it with β-actin gene performance as a reference housekeeping gene. By performing multiple sequence alignments and comparing the highly conserved regions among fish Δ6 Fads sequences, the SBT Δ6 Fads nucleotide sequence was determined to be ≥ 75% identical to the other fish Δ6 Fads sequences. The results showed that when the SBT Δ6 Fads and β-actin cDNAs were performed in a standard PCR system and the products were analysed by electrophoresing them on a 2% (w/v) agarose gel, the target genes that were obtained were similar to the expected sizes. The observed band size for the Δ6 Fads PCR product was 207 bp and for the β-actin PCR product was 98 bp. The presence of the observed bands indicated that the primer pairs that were designed and used were successfully amplified the target genes. The results of this study might provide relevant information to support further investigating of the desaturase and elongase gene expression that might contribute to a better understanding of ω-3 LC-PUFA biosynthesis in fish.
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