A rapid and sensitive Reverse Phase High Performance Liquid Chromatographic [RP-HPLC] method was ... more A rapid and sensitive Reverse Phase High Performance Liquid Chromatographic [RP-HPLC] method was developed for the estimation of Tolperisone and Etodolac 1,2,3 in pure and its tablet dosage forms. The method was validated as per International Conference on Harmonization [ICH] guidelines 35 . A C18 column (250×4.6mm, 5μm) was used with a mobile phase containing a mixture of potassium phosphate monohydrate buffer (pH-2.6) and Acetonitrile in the ratio of 70:30% v/v. The analysis was performed with run time of 6 minutes at a flow rate of 1ml/min. The Tolperisone and Etodolac was monitored at 263nm with UV detection and Tolperisone and Etodolac was eluted at 2.8 min and 4.2 min. The method was linear (r2 =0.999) at concentration ranging from 7.5 to 25μg/ml for Tolperisone and 100-300µg/ml for Etodolac, precise (intra-day relative standard deviation [RSD] and inter-day RSD values < 1.0%), accurate (99.3 to 100.9 for Tolperisone and 100.1 to 100.6 for etodolac), specific and robust. Detection limit of 1.30 for Tolperisone and 1.88 μg/ml for Etodolac. Similarly quantification limits were 3.93 for Tolperisone and 5.70 for Etodolac μg/ml, estimated from linearity by regression respectively. The results showed that the proposed method is suitable for the precise, accurate and rapid determination of Tolperisone and Etodolac in bulk, its combined dosage forms.
A rapid and sensitive Reverse Phase High Performance Liquid Chromatographic [RP-HPLC] method was ... more A rapid and sensitive Reverse Phase High Performance Liquid Chromatographic [RP-HPLC] method was developed for the estimation of Tolperisone and Etodolac 1,2,3 in pure and its tablet dosage forms. The method was validated as per International Conference on Harmonization [ICH] guidelines 35 . A C18 column (250×4.6mm, 5μm) was used with a mobile phase containing a mixture of potassium phosphate monohydrate buffer (pH-2.6) and Acetonitrile in the ratio of 70:30% v/v. The analysis was performed with run time of 6 minutes at a flow rate of 1ml/min. The Tolperisone and Etodolac was monitored at 263nm with UV detection and Tolperisone and Etodolac was eluted at 2.8 min and 4.2 min. The method was linear (r2 =0.999) at concentration ranging from 7.5 to 25μg/ml for Tolperisone and 100-300µg/ml for Etodolac, precise (intra-day relative standard deviation [RSD] and inter-day RSD values < 1.0%), accurate (99.3 to 100.9 for Tolperisone and 100.1 to 100.6 for etodolac), specific and robust. Detection limit of 1.30 for Tolperisone and 1.88 μg/ml for Etodolac. Similarly quantification limits were 3.93 for Tolperisone and 5.70 for Etodolac μg/ml, estimated from linearity by regression respectively. The results showed that the proposed method is suitable for the precise, accurate and rapid determination of Tolperisone and Etodolac in bulk, its combined dosage forms.
A new rapid, precise and sensitive reverse phase high performance liquid chromatographic (RP-HPLC... more A new rapid, precise and sensitive reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed and validated for the estimation of Atazanavir and Ritonavir simultaneously in combined dosage form. The two components Atazanavir and Ritonavir were well resolved on an isocratic method, C18 column, utilizing a mobile phase composition of acetonitrile: 0.02M ammonium acetate buffer (40:60), v/v, pH 4.0) at a flow rate of 1.0 mL/min with UV detection at 205 nm. The retention time of Atazanavir and Ritonavir were 2.8 min and 5.7 min respectively. The developed method was validated for specificity, linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ) and robustness as per ICH guidelines. Linearity for Atazanavir and Ritonavir were found in the range of 18.0-42.0 µg/ml and 5.0-14.0 µg/ml, respectively. The percentage recoveries for Atazanavir and Ritonavir ranged from 98.9-101.0 % and 98.2-100.1 %, respectively. The proposed method could be used for routine analysis of Atazanavir and Ritonavir in their combined dosage forms.
A new rapid, precise and sensitive reverse phase high performance liquid chromatographic (RP-HPLC... more A new rapid, precise and sensitive reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed and validated for the estimation of Perindopril and Amlodipine simultaneously in combined dosage form. The two components Perindopril and Amlodipine were well resolved on an isocratic method, C18 column, utilizing a mobile phase composition of acetonitrile: methanol: a mixed buffer of 0.02M Potassium dihydrogen phosphate buffer and 0.02M Sodium dihydrogen Phosphate buffer with 1mL Tri ethyl amine (40:20:40), v/v, pH 5.0) at a flow rate of 1.0 mL/min with UV detection at 226 nm. The retention time of Perindopril and Amlodipine were 2.9 min and 4.9 min respectively. The developed method was validated for specificity, linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ) and robustness as per ICH guidelines. Linearity for Perindopril and Amlodipine were found in the range of 48-112 µg/ml and 60.0-140.0 µg/ml, respectively. The percentage recoveries for Perindopril and Amlodipine ranged from 98.9-100.4 % and 98.9-100.7 %, respectively. The proposed method could be used for routine analysis of Perindopril and Amlodipine in their combined dosage forms.
A simple accurate and selective Rp-HPLC Cobicistat (COB) and Atazanavir (ATA) 4.6mm) with reverse... more A simple accurate and selective Rp-HPLC Cobicistat (COB) and Atazanavir (ATA) 4.6mm) with reverse phase elution of the mobile phase compose of orthophosphoric acid: MeOH (30:70%v/v) times were 2.53 min for COB and 3.36 100μg/ml respectively with correlation coefficients 0.999. The proposed method statistically validated with respect to system suitability, linearity, precision, accuracy, specificity, robustness, be accurate, precise, specific and rapid found to be suitable for the quantitative analysis of the drug and dosage form.
Cobicistat, Elvitegravir, Phosphate, buffer, methanol A simple, rapid, accurate and precise RP-HP... more Cobicistat, Elvitegravir, Phosphate, buffer, methanol A simple, rapid, accurate and precise RP-HPLC analytical method has been developed and validated for the quantitative analysis of Cobicistat (COB) and Elvitegravir (ELV) in bulk drugs and combined dosage forms. The proposed method was carried out using on an Agilent Xterra C18 (5μm, 15cm X 4.6mm) column. The composition of Mobile phase containing 0.05M Phosphate buffer and methanol (30:70v/v) pH adjusted to 7 with orthophosphoric acid, is reported for the simultaneous estimation of COB and ELV in pharmaceutical dosage forms has been developed. The injection volume of samples was 10μl at 1ml/min flow rate. The column temperature was ambient. UV detection was carried out using a UV-PDA detector at 260 nm. The validation of this method was done as per ICH guidelines. The retention times were observed as 2.39 and 3.90 min for Cobicistat, and Elvitegravir respectively. Linearity ranges were observed at 20 -100 μg/ml for Cobicistat and 10 -50 μg/ml for Elvitegravir. Relative Standard Deviation did not exceed 2. The present study demonstrates the applicability of chromatographic method to develop a new, sensitive, single RP-HPLC method for the simultaneous quantitative determination of two antiviral agents in fixed pharmaceutical dosage form.
A rapid and sensitive Reverse Phase High Performance Liquid Chromatographic [RP-HPLC] method was ... more A rapid and sensitive Reverse Phase High Performance Liquid Chromatographic [RP-HPLC] method was developed for the estimation of Tolperisone and Etodolac 1,2,3 in pure and its tablet dosage forms. The method was validated as per International Conference on Harmonization [ICH] guidelines 35 . A C18 column (250×4.6mm, 5μm) was used with a mobile phase containing a mixture of potassium phosphate monohydrate buffer (pH-2.6) and Acetonitrile in the ratio of 70:30% v/v. The analysis was performed with run time of 6 minutes at a flow rate of 1ml/min. The Tolperisone and Etodolac was monitored at 263nm with UV detection and Tolperisone and Etodolac was eluted at 2.8 min and 4.2 min. The method was linear (r2 =0.999) at concentration ranging from 7.5 to 25μg/ml for Tolperisone and 100-300µg/ml for Etodolac, precise (intra-day relative standard deviation [RSD] and inter-day RSD values < 1.0%), accurate (99.3 to 100.9 for Tolperisone and 100.1 to 100.6 for etodolac), specific and robust. Detection limit of 1.30 for Tolperisone and 1.88 μg/ml for Etodolac. Similarly quantification limits were 3.93 for Tolperisone and 5.70 for Etodolac μg/ml, estimated from linearity by regression respectively. The results showed that the proposed method is suitable for the precise, accurate and rapid determination of Tolperisone and Etodolac in bulk, its combined dosage forms.
A rapid and sensitive Reverse Phase High Performance Liquid Chromatographic [RP-HPLC] method was ... more A rapid and sensitive Reverse Phase High Performance Liquid Chromatographic [RP-HPLC] method was developed for the estimation of Tolperisone and Etodolac 1,2,3 in pure and its tablet dosage forms. The method was validated as per International Conference on Harmonization [ICH] guidelines 35 . A C18 column (250×4.6mm, 5μm) was used with a mobile phase containing a mixture of potassium phosphate monohydrate buffer (pH-2.6) and Acetonitrile in the ratio of 70:30% v/v. The analysis was performed with run time of 6 minutes at a flow rate of 1ml/min. The Tolperisone and Etodolac was monitored at 263nm with UV detection and Tolperisone and Etodolac was eluted at 2.8 min and 4.2 min. The method was linear (r2 =0.999) at concentration ranging from 7.5 to 25μg/ml for Tolperisone and 100-300µg/ml for Etodolac, precise (intra-day relative standard deviation [RSD] and inter-day RSD values < 1.0%), accurate (99.3 to 100.9 for Tolperisone and 100.1 to 100.6 for etodolac), specific and robust. Detection limit of 1.30 for Tolperisone and 1.88 μg/ml for Etodolac. Similarly quantification limits were 3.93 for Tolperisone and 5.70 for Etodolac μg/ml, estimated from linearity by regression respectively. The results showed that the proposed method is suitable for the precise, accurate and rapid determination of Tolperisone and Etodolac in bulk, its combined dosage forms.
A new rapid, precise and sensitive reverse phase high performance liquid chromatographic (RP-HPLC... more A new rapid, precise and sensitive reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed and validated for the estimation of Atazanavir and Ritonavir simultaneously in combined dosage form. The two components Atazanavir and Ritonavir were well resolved on an isocratic method, C18 column, utilizing a mobile phase composition of acetonitrile: 0.02M ammonium acetate buffer (40:60), v/v, pH 4.0) at a flow rate of 1.0 mL/min with UV detection at 205 nm. The retention time of Atazanavir and Ritonavir were 2.8 min and 5.7 min respectively. The developed method was validated for specificity, linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ) and robustness as per ICH guidelines. Linearity for Atazanavir and Ritonavir were found in the range of 18.0-42.0 µg/ml and 5.0-14.0 µg/ml, respectively. The percentage recoveries for Atazanavir and Ritonavir ranged from 98.9-101.0 % and 98.2-100.1 %, respectively. The proposed method could be used for routine analysis of Atazanavir and Ritonavir in their combined dosage forms.
A new rapid, precise and sensitive reverse phase high performance liquid chromatographic (RP-HPLC... more A new rapid, precise and sensitive reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed and validated for the estimation of Perindopril and Amlodipine simultaneously in combined dosage form. The two components Perindopril and Amlodipine were well resolved on an isocratic method, C18 column, utilizing a mobile phase composition of acetonitrile: methanol: a mixed buffer of 0.02M Potassium dihydrogen phosphate buffer and 0.02M Sodium dihydrogen Phosphate buffer with 1mL Tri ethyl amine (40:20:40), v/v, pH 5.0) at a flow rate of 1.0 mL/min with UV detection at 226 nm. The retention time of Perindopril and Amlodipine were 2.9 min and 4.9 min respectively. The developed method was validated for specificity, linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ) and robustness as per ICH guidelines. Linearity for Perindopril and Amlodipine were found in the range of 48-112 µg/ml and 60.0-140.0 µg/ml, respectively. The percentage recoveries for Perindopril and Amlodipine ranged from 98.9-100.4 % and 98.9-100.7 %, respectively. The proposed method could be used for routine analysis of Perindopril and Amlodipine in their combined dosage forms.
A simple accurate and selective Rp-HPLC Cobicistat (COB) and Atazanavir (ATA) 4.6mm) with reverse... more A simple accurate and selective Rp-HPLC Cobicistat (COB) and Atazanavir (ATA) 4.6mm) with reverse phase elution of the mobile phase compose of orthophosphoric acid: MeOH (30:70%v/v) times were 2.53 min for COB and 3.36 100μg/ml respectively with correlation coefficients 0.999. The proposed method statistically validated with respect to system suitability, linearity, precision, accuracy, specificity, robustness, be accurate, precise, specific and rapid found to be suitable for the quantitative analysis of the drug and dosage form.
Cobicistat, Elvitegravir, Phosphate, buffer, methanol A simple, rapid, accurate and precise RP-HP... more Cobicistat, Elvitegravir, Phosphate, buffer, methanol A simple, rapid, accurate and precise RP-HPLC analytical method has been developed and validated for the quantitative analysis of Cobicistat (COB) and Elvitegravir (ELV) in bulk drugs and combined dosage forms. The proposed method was carried out using on an Agilent Xterra C18 (5μm, 15cm X 4.6mm) column. The composition of Mobile phase containing 0.05M Phosphate buffer and methanol (30:70v/v) pH adjusted to 7 with orthophosphoric acid, is reported for the simultaneous estimation of COB and ELV in pharmaceutical dosage forms has been developed. The injection volume of samples was 10μl at 1ml/min flow rate. The column temperature was ambient. UV detection was carried out using a UV-PDA detector at 260 nm. The validation of this method was done as per ICH guidelines. The retention times were observed as 2.39 and 3.90 min for Cobicistat, and Elvitegravir respectively. Linearity ranges were observed at 20 -100 μg/ml for Cobicistat and 10 -50 μg/ml for Elvitegravir. Relative Standard Deviation did not exceed 2. The present study demonstrates the applicability of chromatographic method to develop a new, sensitive, single RP-HPLC method for the simultaneous quantitative determination of two antiviral agents in fixed pharmaceutical dosage form.
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Papers by Srinivas Ganta