Soybean extract is a valuable food supplement used for the prevention of cancer and other purpose... more Soybean extract is a valuable food supplement used for the prevention of cancer and other purposes. The role of free radicals in carcinogenesis has been proven. Studies have also confirmed the effect of storage conditions on the formation of free radicals in natural medicinal products. The study presents an attempt to optimize the storage conditions of soybean extract to prevent the formation of free radicals. The aim of the study was to determine the effect of temperature and UV radiation on the formation of free radicals in soybean extract during storage. Samples of soybean extract were heated to temperatures of 30, 40 and 50°C. Subsequent samples were exposed to UV radiation (wavelengths 315-400 nm) for 30 and 60 minutes. EPR (electron paramagnetic resonance) spectroscopy was used to analyse the amplitude and linewidth of the EPR signal and to calculate the concentration of free radicals in the samples.
Annales Academiae Medicae Silesiensis, Jun 9, 2017
Soybean extract is a valuable food supplement used for the prevention of cancer and other purpose... more Soybean extract is a valuable food supplement used for the prevention of cancer and other purposes. The role of free radicals in carcinogenesis has been proven. Studies have also confirmed the effect of storage conditions on the formation of free radicals in natural medicinal products. The study presents an attempt to optimize the storage conditions of soybean extract to prevent the formation of free radicals. The aim of the study was to determine the effect of temperature and UV radiation on the formation of free radicals in soybean extract during storage. Samples of soybean extract were heated to temperatures of 30, 40 and 50°C. Subsequent samples were exposed to UV radiation (wavelengths 315-400 nm) for 30 and 60 minutes. EPR (electron paramagnetic resonance) spectroscopy was used to analyse the amplitude and linewidth of the EPR signal and to calculate the concentration of free radicals in the samples.
Rosuvastatin was thermally sterilized at temperatures 160⁰C during 120 min, 170⁰C during 60 min a... more Rosuvastatin was thermally sterilized at temperatures 160⁰C during 120 min, 170⁰C during 60 min and 180⁰C during 30 min, according to Polish pharmacopoeia norms. Free radicals formed in the heated rosuvastatin samples were examined by electron paramagnetic resonance (EPR) spectroscopy. The best conditions of thermal sterilization of rosuvastatin were searched. EPR spectra were measured for the analyzed drug independent on heating temperature. Decrease of free radical concentrations in rosuvastatin with increasing of heating temperature was stated. The optimal conditions for thermal sterilization of rosuvastatin were temperature 180⁰C and heating time of 30 min and resulted in the lowest free radical concentration. The changes of amplitudes (A) and linewidths (∇Bpp) of EPR spectra of the heated rosuvastatin were characteristic for homogeneous broadening EPR lines. Slow spin-lattice relaxation processes existed in the all rosuvastatin samples. The confirmation of usefulness of electron paramagnetic resonance spectroscopy in pharmacy was presented.
An X-band (9.3 GHz) electron paramagnetic resonance (EPR) spectroscopy was used to examine the fr... more An X-band (9.3 GHz) electron paramagnetic resonance (EPR) spectroscopy was used to examine the free radical scavenging activity of the following infusions, which were nonirradiated and exposed to UVA: root of Asparagus racemosus and herbs of Mitchella repens, Cnicus benedictus L., Galega officinalis L., and Eupatorium cannabinum L. The plant materials for obstetrics applications were chosen for analysis. The aims of these studies were to compare the free radical scavenging ability of the tested infusions and to determine the influence of UVA irradiation of the plant materials on interactions of these infusions with free radicals. Both the magnitude and kinetics of the interactions of the infusions with the model DPPH free radicals were examined. The ability to quench the free radicals for the examined plant infusions increases in the following order: Asparagus racemosus (root) < Mitchella repens (herb) < Cnicus benedictus L. (herb) < Galega officinalis L. (herb) < Eupato...
Rosuvastatin was thermally sterilized at temperatures 160⁰C during 120 min, 170⁰C during 60 min a... more Rosuvastatin was thermally sterilized at temperatures 160⁰C during 120 min, 170⁰C during 60 min and 180⁰C during 30 min, according to Polish pharmacopoeia norms. Free radicals formed in the heated rosuvastatin samples were examined by electron paramagnetic resonance (EPR) spectroscopy. The best conditions of thermal sterilization of rosuvastatin were searched. EPR spectra were measured for the analyzed drug independent on heating temperature. Decrease of free radical concentrations in rosuvastatin with increasing of heating temperature was stated. The optimal conditions for thermal sterilization of rosuvastatin were temperature 180⁰C and heating time of 30 min and resulted in the lowest free radical concentration. The changes of amplitudes (A) and linewidths (∇Bpp) of EPR spectra of the heated rosuvastatin were characteristic for homogeneous broadening EPR lines. Slow spin-lattice relaxation processes existed in the all rosuvastatin samples. The confirmation of usefulness of electro...
Soybean extract is a valuable food supplement used for the prevention of cancer and other purpose... more Soybean extract is a valuable food supplement used for the prevention of cancer and other purposes. The role of free radicals in carcinogenesis has been proven. Studies have also confirmed the effect of storage conditions on the formation of free radicals in natural medicinal products. The study presents an attempt to optimize the storage conditions of soybean extract to prevent the formation of free radicals. The aim of the study was to determine the effect of temperature and UV radiation on the formation of free radicals in soybean extract during storage. Samples of soybean extract were heated to temperatures of 30, 40 and 50°C. Subsequent samples were exposed to UV radiation (wavelengths 315-400 nm) for 30 and 60 minutes. EPR (electron paramagnetic resonance) spectroscopy was used to analyse the amplitude and linewidth of the EPR signal and to calculate the concentration of free radicals in the samples.
Annales Academiae Medicae Silesiensis, Jun 9, 2017
Soybean extract is a valuable food supplement used for the prevention of cancer and other purpose... more Soybean extract is a valuable food supplement used for the prevention of cancer and other purposes. The role of free radicals in carcinogenesis has been proven. Studies have also confirmed the effect of storage conditions on the formation of free radicals in natural medicinal products. The study presents an attempt to optimize the storage conditions of soybean extract to prevent the formation of free radicals. The aim of the study was to determine the effect of temperature and UV radiation on the formation of free radicals in soybean extract during storage. Samples of soybean extract were heated to temperatures of 30, 40 and 50°C. Subsequent samples were exposed to UV radiation (wavelengths 315-400 nm) for 30 and 60 minutes. EPR (electron paramagnetic resonance) spectroscopy was used to analyse the amplitude and linewidth of the EPR signal and to calculate the concentration of free radicals in the samples.
Rosuvastatin was thermally sterilized at temperatures 160⁰C during 120 min, 170⁰C during 60 min a... more Rosuvastatin was thermally sterilized at temperatures 160⁰C during 120 min, 170⁰C during 60 min and 180⁰C during 30 min, according to Polish pharmacopoeia norms. Free radicals formed in the heated rosuvastatin samples were examined by electron paramagnetic resonance (EPR) spectroscopy. The best conditions of thermal sterilization of rosuvastatin were searched. EPR spectra were measured for the analyzed drug independent on heating temperature. Decrease of free radical concentrations in rosuvastatin with increasing of heating temperature was stated. The optimal conditions for thermal sterilization of rosuvastatin were temperature 180⁰C and heating time of 30 min and resulted in the lowest free radical concentration. The changes of amplitudes (A) and linewidths (∇Bpp) of EPR spectra of the heated rosuvastatin were characteristic for homogeneous broadening EPR lines. Slow spin-lattice relaxation processes existed in the all rosuvastatin samples. The confirmation of usefulness of electron paramagnetic resonance spectroscopy in pharmacy was presented.
An X-band (9.3 GHz) electron paramagnetic resonance (EPR) spectroscopy was used to examine the fr... more An X-band (9.3 GHz) electron paramagnetic resonance (EPR) spectroscopy was used to examine the free radical scavenging activity of the following infusions, which were nonirradiated and exposed to UVA: root of Asparagus racemosus and herbs of Mitchella repens, Cnicus benedictus L., Galega officinalis L., and Eupatorium cannabinum L. The plant materials for obstetrics applications were chosen for analysis. The aims of these studies were to compare the free radical scavenging ability of the tested infusions and to determine the influence of UVA irradiation of the plant materials on interactions of these infusions with free radicals. Both the magnitude and kinetics of the interactions of the infusions with the model DPPH free radicals were examined. The ability to quench the free radicals for the examined plant infusions increases in the following order: Asparagus racemosus (root) < Mitchella repens (herb) < Cnicus benedictus L. (herb) < Galega officinalis L. (herb) < Eupato...
Rosuvastatin was thermally sterilized at temperatures 160⁰C during 120 min, 170⁰C during 60 min a... more Rosuvastatin was thermally sterilized at temperatures 160⁰C during 120 min, 170⁰C during 60 min and 180⁰C during 30 min, according to Polish pharmacopoeia norms. Free radicals formed in the heated rosuvastatin samples were examined by electron paramagnetic resonance (EPR) spectroscopy. The best conditions of thermal sterilization of rosuvastatin were searched. EPR spectra were measured for the analyzed drug independent on heating temperature. Decrease of free radical concentrations in rosuvastatin with increasing of heating temperature was stated. The optimal conditions for thermal sterilization of rosuvastatin were temperature 180⁰C and heating time of 30 min and resulted in the lowest free radical concentration. The changes of amplitudes (A) and linewidths (∇Bpp) of EPR spectra of the heated rosuvastatin were characteristic for homogeneous broadening EPR lines. Slow spin-lattice relaxation processes existed in the all rosuvastatin samples. The confirmation of usefulness of electro...
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