Ž. Ž. Halibut, Hippoglossus hippoglossus L. and Atlantic salmon, Salmo salar L. were challenged w... more Ž. Ž. Halibut, Hippoglossus hippoglossus L. and Atlantic salmon, Salmo salar L. were challenged with virulent typical or atypical strains of Aeromonas salmonicida by both injection and bath models. The groups were injected intraperitoneally with 100 ml of logarithmically decreasing Ž 8 3. 5 dilutions of A. salmonicida range 10-10 cellsrfish or bathed in 10 CFUsrml of A. salmonicida for 24 h. Halibut were significantly more resistant to infection, compared to Atlantic salmon, with 10 6 and 10 7 typical and atypical A. salmonicida cellsrhalibut being the minimum lethal dose. No halibut died in the bath challenge although approximately 80% of salmon died by this challenge method. All animals that died during the challenge were positive for A. salmonicida on culture A. salmonicida was cultured from internal organs of approximately 60% of surviving Atlantic salmon while only 2.3% of the surviving halibut were culture positive, but only from the intestinal lumen. All surviving salmon but none of the surviving halibut, showed histological evidence of infection with A. salmonicida. Stress tests of both halibut and Atlantic salmon showed that recovered salmon still had a high carrier rate of A. salmonicida while none of the surviving halibut were carrier test positive. A significant rise in agglutinating antibody titre was observed in surviving Atlantic salmon. However, no rise in antibody titre was observed in the surviving halibut, suggesting that the processing of the bacteria during an acute infection may be fundamentally different between these two species.
The trypsin-inhibitory activity of the serum of five cultured fish species, halibut (Hippoglossus... more The trypsin-inhibitory activity of the serum of five cultured fish species, halibut (Hippoglossus hippoglossus L.), turbot (Scophthalmus maximus L.), arctic char (Salvelinus alpinus L.), rainbow trout (Oncorhynchus mykiss, Walbaum) and brown trout (Salmo trutta L.) was investigated. Individual fish were bled and the serum trypsin-inhibitory activity analysed using an arginine-aniline dye ester as colorimetric substrate. Results for the five species indicate that levels of 2-macroglobulin-like activity decrease significantly (P<0•05) from halibut through turbot, brown trout, char and finally rainbow trout with the lowest levels. Analysis of the total trypsin binding capacity indicates a ranking with decreasing concentration of char, turbot, halibut, brown trout and rainbow trout. Significant di#erences occurred only in certain cases, brown and rainbow trout were significantly (P<0•05) lower than char, and rainbow trout was significantly (P<0•05) lower than turbot. The results indicate that halibut and turbot possess a higher level of 2-macroglobulin-like activity than the other species in the study, whereas char had the highest total trypsin-inhibitory level.
Ž. Ž. Halibut, Hippoglossus hippoglossus L. and Atlantic salmon, Salmo salar L. were challenged w... more Ž. Ž. Halibut, Hippoglossus hippoglossus L. and Atlantic salmon, Salmo salar L. were challenged with virulent typical or atypical strains of Aeromonas salmonicida by both injection and bath models. The groups were injected intraperitoneally with 100 ml of logarithmically decreasing Ž 8 3. 5 dilutions of A. salmonicida range 10-10 cellsrfish or bathed in 10 CFUsrml of A. salmonicida for 24 h. Halibut were significantly more resistant to infection, compared to Atlantic salmon, with 10 6 and 10 7 typical and atypical A. salmonicida cellsrhalibut being the minimum lethal dose. No halibut died in the bath challenge although approximately 80% of salmon died by this challenge method. All animals that died during the challenge were positive for A. salmonicida on culture A. salmonicida was cultured from internal organs of approximately 60% of surviving Atlantic salmon while only 2.3% of the surviving halibut were culture positive, but only from the intestinal lumen. All surviving salmon but none of the surviving halibut, showed histological evidence of infection with A. salmonicida. Stress tests of both halibut and Atlantic salmon showed that recovered salmon still had a high carrier rate of A. salmonicida while none of the surviving halibut were carrier test positive. A significant rise in agglutinating antibody titre was observed in surviving Atlantic salmon. However, no rise in antibody titre was observed in the surviving halibut, suggesting that the processing of the bacteria during an acute infection may be fundamentally different between these two species.
The trypsin-inhibitory activity of the serum of five cultured fish species, halibut (Hippoglossus... more The trypsin-inhibitory activity of the serum of five cultured fish species, halibut (Hippoglossus hippoglossus L.), turbot (Scophthalmus maximus L.), arctic char (Salvelinus alpinus L.), rainbow trout (Oncorhynchus mykiss, Walbaum) and brown trout (Salmo trutta L.) was investigated. Individual fish were bled and the serum trypsin-inhibitory activity analysed using an arginine-aniline dye ester as colorimetric substrate. Results for the five species indicate that levels of 2-macroglobulin-like activity decrease significantly (P<0•05) from halibut through turbot, brown trout, char and finally rainbow trout with the lowest levels. Analysis of the total trypsin binding capacity indicates a ranking with decreasing concentration of char, turbot, halibut, brown trout and rainbow trout. Significant di#erences occurred only in certain cases, brown and rainbow trout were significantly (P<0•05) lower than char, and rainbow trout was significantly (P<0•05) lower than turbot. The results indicate that halibut and turbot possess a higher level of 2-macroglobulin-like activity than the other species in the study, whereas char had the highest total trypsin-inhibitory level.
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