Nanoparticles (NPs) have provided significant advances in science in the last few decades. NPs de... more Nanoparticles (NPs) have provided significant advances in science in the last few decades. NPs demonstrates wide versatility and the use and green nanotechnology has been an alternative for the production of low-cost, energy-efficient, and nontoxic. This review provides an overview of a few studies using metallic NPs from green synthesis.
Infectious diseases are a worldwide concern. They are responsible for increasing the mortality ra... more Infectious diseases are a worldwide concern. They are responsible for increasing the mortality rate and causing economic and social problems. Viral epidemics and pandemics, such as the COVID-19 pan...
The consumption of high-protein diets (HPD) is associated with resistance training (RT) due to ef... more The consumption of high-protein diets (HPD) is associated with resistance training (RT) due to effects on metabolism. However, little is known about these effects on cardiac tissue. This study aimed to investigate effects of HPD and RT on cardiac biomarkers. 18 rats were divided into normo-protein (NPD), and HPD groups: NPD-Control, NPD-RT, HPD-Control, and HPD-RT. Interleukin-6 (IL-6), tumour necrosis factor alpha (TNF-a), nitric oxide (NO), activity of metalloproteinase-2 (MMP-2), and vascular factor (VEGF) were analysed. RT was effective in regulating body weight, increasing strength, and reducing food consumption (p < .05). HPD induces higher levels of interleukin 6 (p = .0169), and lowers NO (p < .0001). When associated with RT, the HPD decreases levels of tumour necrosis factor alpha, while enhances NO, and MMP activity (p < .05). The association of RT with HDP decreases inflammatory parameters and indicates an enhancement in the molecular parameters of cardiac tissue.
Antimicrobial agents and chemotherapy, Aug 30, 2017
Fungal Candida species are commensals present in the mammalian skin and mucous membranes. Candida... more Fungal Candida species are commensals present in the mammalian skin and mucous membranes. Candida spp. are capable of breaching the epithelial barrier of immuno-compromised patients with neutrophil and cell-mediated immune dysfunctions, and it can also disseminate to multiple organs through the bloodstream. Here we examined the action of innate defence regulator (IDR)-1018, a 12-aminoacid residue peptide derived from bovine bactenecin (Bac2A), showed weak antifungal and anti-biofilm activity against C. albicans laboratory (ATCC 10231) and a clinical isolate (CI) strains (MICs of 32 μg.mL(-1) and 64 μg.mL(-1), respectively), while 8-fold lower concentrations led to dissolution of the fungal cells from pre-formed biofilms. IDR-1018 at 128 μg.mL(-1) was not hemolytic when tested against murine red blood cells, and also has not shown cytotoxic effect on murine monocyte RAW 264.7 and primary murine macrophage cells at the tested concentrations. IDR-1018 modulated the cytokine profile due...
O agregado de trioxido mineral (MTA), por ser um cimento retrobturador utilizado em regioes infla... more O agregado de trioxido mineral (MTA), por ser um cimento retrobturador utilizado em regioes inflamadas e/ou infectadas, deve ser biocompativel e nao interferir na atividade dos macrofagos. Recentes estudos diferenciam os macrofagos de acordo com o tipo de resposta: M1 (ativados pela producao de IL-12); M2 (ativados alternativamente pela ausencia de IL-12). Neste estudo, testou-se o efeito de duas marcas comerciais do MTA (ProRoot, gentilmente cedido pela Tulsa Dental, Ballaigues, Suica e MTA-Ângelus, gentilmente cedido pela Odonto-logika, Londrina, Brasil) sobre a atividade de macrofagos inflamatorios peritoneais M1 (provenientes de camundongos C57BL/6) e M2 (provenientes de camundongos C57BL/6 IL-12p40-/-). Foram avaliadas: a viabilidade e aderencia celular, a fagocitose da Saccharomyces boulardii, a producao de ROIs, quando os macrofagos foram estimulados e nao com zymosan, e a producao de TNF, IL-12, IL-10 e NO, quando estimulados e nao com Fusobacterium nucleatun, Peptostreptococcus anaerobious, com e sem IFN-. Os cimentos nao interferiram nos parâmetros analisados da atividade dos macrofagos M1 e M2. Porem, quando se compararam os macrofagos M2 com os M1, pode-se observar: os macrofagos M2 sobreviveram menos, nos experimentos de viabilidade celular em tubos de polipropileno; apresentaram maior numero de leveduras fagocitadas; tiveram maior producao de IL-10, quando estimulados com F. nucleatum; produziram menos ROIs. Nos demais parâmetros analisados, nao houve diferencas significativas entre ambos. Conclui-se que os cimentos nao inibem a resposta pro-inflamatoria dos macrofagos M1 e M2; as respostas desses dois tipos celulares diferenciam-se em alguns aspectos.
Perfuracao radicular (PR) e um acidente que promove uma comunicacao do canal radicular com o peri... more Perfuracao radicular (PR) e um acidente que promove uma comunicacao do canal radicular com o periodonto, sendo responsavel por uma taxa consideravel de insucesso nos tratamentos endodonticos. Diversos fatores influenciam o prognostico das PRs: tamanho e localizacao da comunicacao, facilidade de acesso, tempo decorrido entre sua ocorrencia e o seu selamento, presenca de contaminacao microbiana e material utilizado para o seu preenchimento. O objetivo deste trabalho foi avaliar os casos de PRs ocorridos entre os anos de 2006 a 2011, nas Clinicas Integradas de Odontologia da Universidade Catolica de Brasilia (UCB). Foram contabilizados 2036 prontuarios correspondente ao numero de pacientes atendidos entre os anos de 2006 a 2011. Os prontuarios que apresentaram planejamento de tratamento endodontico ou de retentores intra-radiculares nas fases de urgencia, adequacao do meio, reabilitadora ou de manutencao foram avaliados atraves de detalhada leitura da evolucao do tratamento. Nos relatos de PRs foram analisados: data de ocorrencia do acidente, presenca de selamento e descricao do material utilizado, presenca ou ausencia de acompanhamento e o prognostico do caso clinico. Em momento posterior ao levantamento de dados, os pacientes que apresentaram historico de PR, sem indicacao de exodontia foram convocados para avaliacao clinica e radiografica de sucesso e/ou insucesso do tratamento. Foram encontradas 14 ocorrencias de PRs, porem apenas 7 casos foram reavaliados, devido a impossibilidade dos demais pacientes em comparecer ao local da pesquisa. Destas perfuracoes, 4 casos foram selados, enquanto 3 nao o foram. O tratamento implementado nestes casos, envolveu obturacao convencional dos canais radiculares em 2 casos, curativo intra-canal em 1 caso e indicacao a exodontia em outros 4 casos. O indice de sucesso foi de 43%, enquanto o insucesso ficou em torno de 57%. As causas de insucesso relatadas foram falta de suporte para reabilitacao protetica em 2 casos e perda de insercao em outros 2. Conclui-se que o correto conhecimento da anatomia radicular, a correta interpretacao da radiografia de diagnostico e a correta execucao da tecnica sao de fundamental importância para se evitar a ocorrencia de PRs. Por fim, foi possivel observar que a correta descricao desses acidentes no prontuario, nem sempre ocorre.
The spread of multi-drug resistance and the slow pace at which antibiotics come onto the market a... more The spread of multi-drug resistance and the slow pace at which antibiotics come onto the market are undermining our ability to treat human infections, leading to high mortality rates. Aiming to overcome this global crisis, antimicrobial peptides are considered promising alternatives to counter bacterial infections with multi-drug resistant bacteria. The cathelicidins comprise a well-studied class of AMPs whose members have been used as model molecules for sequence modifications, aiming at enhanced biological activities and stability, along with reduced toxic effects on mammalian cells. Here, we describe the antimicrobial activities, modes of action and structural characterization of two novel cathelicidin-like peptides, named BotrAMP14 and CrotAMP14, which were re-designed from snake batroxicidin and crotalicidin, respectively. BotrAMP14 and CrotAMP14 showed broad-spectrum antibacterial activity against susceptible microorganisms and clinical isolates with minimal inhibitory concent...
Aim: Nitric oxide (NO) is an important mediator related to damage of the pulp tissue and at the s... more Aim: Nitric oxide (NO) is an important mediator related to damage of the pulp tissue and at the same time to regenerative pulp processes. However, it is not clear how common endodontic microorganisms can regulate this mediator. This study aimed to investigate NO production by macrophages and fibroblasts against Enterococcus faecalis- and Staphylococcus aureus-antigens. Methods: RAW 264.7 macrophages and L929 fibroblast cell lines were stimulated with different heat-killed (HK) antigen concentrations (105-108 colony forming units - CFU) from E. faecalis and S. aureus with or without interferon-gamma (IFN-γ). Cell viability by MTT colorimetric assay and NO production from the culture supernatants were evaluated after 72 h. Results: Data here reported demonstrated that none of the antigen concentrations decreased cell viability in macrophages and fibroblasts. The presence of HK-S. aureus and HK-E. faecalis antigen- stimulated NO production with or without IFN-γ on RAW 264.7. The HK-S. ...
Nanoparticles (NPs) have provided significant advances in science in the last few decades. NPs de... more Nanoparticles (NPs) have provided significant advances in science in the last few decades. NPs demonstrates wide versatility and the use and green nanotechnology has been an alternative for the production of low-cost, energy-efficient, and nontoxic. This review provides an overview of a few studies using metallic NPs from green synthesis.
Infectious diseases are a worldwide concern. They are responsible for increasing the mortality ra... more Infectious diseases are a worldwide concern. They are responsible for increasing the mortality rate and causing economic and social problems. Viral epidemics and pandemics, such as the COVID-19 pan...
The consumption of high-protein diets (HPD) is associated with resistance training (RT) due to ef... more The consumption of high-protein diets (HPD) is associated with resistance training (RT) due to effects on metabolism. However, little is known about these effects on cardiac tissue. This study aimed to investigate effects of HPD and RT on cardiac biomarkers. 18 rats were divided into normo-protein (NPD), and HPD groups: NPD-Control, NPD-RT, HPD-Control, and HPD-RT. Interleukin-6 (IL-6), tumour necrosis factor alpha (TNF-a), nitric oxide (NO), activity of metalloproteinase-2 (MMP-2), and vascular factor (VEGF) were analysed. RT was effective in regulating body weight, increasing strength, and reducing food consumption (p < .05). HPD induces higher levels of interleukin 6 (p = .0169), and lowers NO (p < .0001). When associated with RT, the HPD decreases levels of tumour necrosis factor alpha, while enhances NO, and MMP activity (p < .05). The association of RT with HDP decreases inflammatory parameters and indicates an enhancement in the molecular parameters of cardiac tissue.
Antimicrobial agents and chemotherapy, Aug 30, 2017
Fungal Candida species are commensals present in the mammalian skin and mucous membranes. Candida... more Fungal Candida species are commensals present in the mammalian skin and mucous membranes. Candida spp. are capable of breaching the epithelial barrier of immuno-compromised patients with neutrophil and cell-mediated immune dysfunctions, and it can also disseminate to multiple organs through the bloodstream. Here we examined the action of innate defence regulator (IDR)-1018, a 12-aminoacid residue peptide derived from bovine bactenecin (Bac2A), showed weak antifungal and anti-biofilm activity against C. albicans laboratory (ATCC 10231) and a clinical isolate (CI) strains (MICs of 32 μg.mL(-1) and 64 μg.mL(-1), respectively), while 8-fold lower concentrations led to dissolution of the fungal cells from pre-formed biofilms. IDR-1018 at 128 μg.mL(-1) was not hemolytic when tested against murine red blood cells, and also has not shown cytotoxic effect on murine monocyte RAW 264.7 and primary murine macrophage cells at the tested concentrations. IDR-1018 modulated the cytokine profile due...
O agregado de trioxido mineral (MTA), por ser um cimento retrobturador utilizado em regioes infla... more O agregado de trioxido mineral (MTA), por ser um cimento retrobturador utilizado em regioes inflamadas e/ou infectadas, deve ser biocompativel e nao interferir na atividade dos macrofagos. Recentes estudos diferenciam os macrofagos de acordo com o tipo de resposta: M1 (ativados pela producao de IL-12); M2 (ativados alternativamente pela ausencia de IL-12). Neste estudo, testou-se o efeito de duas marcas comerciais do MTA (ProRoot, gentilmente cedido pela Tulsa Dental, Ballaigues, Suica e MTA-Ângelus, gentilmente cedido pela Odonto-logika, Londrina, Brasil) sobre a atividade de macrofagos inflamatorios peritoneais M1 (provenientes de camundongos C57BL/6) e M2 (provenientes de camundongos C57BL/6 IL-12p40-/-). Foram avaliadas: a viabilidade e aderencia celular, a fagocitose da Saccharomyces boulardii, a producao de ROIs, quando os macrofagos foram estimulados e nao com zymosan, e a producao de TNF, IL-12, IL-10 e NO, quando estimulados e nao com Fusobacterium nucleatun, Peptostreptococcus anaerobious, com e sem IFN-. Os cimentos nao interferiram nos parâmetros analisados da atividade dos macrofagos M1 e M2. Porem, quando se compararam os macrofagos M2 com os M1, pode-se observar: os macrofagos M2 sobreviveram menos, nos experimentos de viabilidade celular em tubos de polipropileno; apresentaram maior numero de leveduras fagocitadas; tiveram maior producao de IL-10, quando estimulados com F. nucleatum; produziram menos ROIs. Nos demais parâmetros analisados, nao houve diferencas significativas entre ambos. Conclui-se que os cimentos nao inibem a resposta pro-inflamatoria dos macrofagos M1 e M2; as respostas desses dois tipos celulares diferenciam-se em alguns aspectos.
Perfuracao radicular (PR) e um acidente que promove uma comunicacao do canal radicular com o peri... more Perfuracao radicular (PR) e um acidente que promove uma comunicacao do canal radicular com o periodonto, sendo responsavel por uma taxa consideravel de insucesso nos tratamentos endodonticos. Diversos fatores influenciam o prognostico das PRs: tamanho e localizacao da comunicacao, facilidade de acesso, tempo decorrido entre sua ocorrencia e o seu selamento, presenca de contaminacao microbiana e material utilizado para o seu preenchimento. O objetivo deste trabalho foi avaliar os casos de PRs ocorridos entre os anos de 2006 a 2011, nas Clinicas Integradas de Odontologia da Universidade Catolica de Brasilia (UCB). Foram contabilizados 2036 prontuarios correspondente ao numero de pacientes atendidos entre os anos de 2006 a 2011. Os prontuarios que apresentaram planejamento de tratamento endodontico ou de retentores intra-radiculares nas fases de urgencia, adequacao do meio, reabilitadora ou de manutencao foram avaliados atraves de detalhada leitura da evolucao do tratamento. Nos relatos de PRs foram analisados: data de ocorrencia do acidente, presenca de selamento e descricao do material utilizado, presenca ou ausencia de acompanhamento e o prognostico do caso clinico. Em momento posterior ao levantamento de dados, os pacientes que apresentaram historico de PR, sem indicacao de exodontia foram convocados para avaliacao clinica e radiografica de sucesso e/ou insucesso do tratamento. Foram encontradas 14 ocorrencias de PRs, porem apenas 7 casos foram reavaliados, devido a impossibilidade dos demais pacientes em comparecer ao local da pesquisa. Destas perfuracoes, 4 casos foram selados, enquanto 3 nao o foram. O tratamento implementado nestes casos, envolveu obturacao convencional dos canais radiculares em 2 casos, curativo intra-canal em 1 caso e indicacao a exodontia em outros 4 casos. O indice de sucesso foi de 43%, enquanto o insucesso ficou em torno de 57%. As causas de insucesso relatadas foram falta de suporte para reabilitacao protetica em 2 casos e perda de insercao em outros 2. Conclui-se que o correto conhecimento da anatomia radicular, a correta interpretacao da radiografia de diagnostico e a correta execucao da tecnica sao de fundamental importância para se evitar a ocorrencia de PRs. Por fim, foi possivel observar que a correta descricao desses acidentes no prontuario, nem sempre ocorre.
The spread of multi-drug resistance and the slow pace at which antibiotics come onto the market a... more The spread of multi-drug resistance and the slow pace at which antibiotics come onto the market are undermining our ability to treat human infections, leading to high mortality rates. Aiming to overcome this global crisis, antimicrobial peptides are considered promising alternatives to counter bacterial infections with multi-drug resistant bacteria. The cathelicidins comprise a well-studied class of AMPs whose members have been used as model molecules for sequence modifications, aiming at enhanced biological activities and stability, along with reduced toxic effects on mammalian cells. Here, we describe the antimicrobial activities, modes of action and structural characterization of two novel cathelicidin-like peptides, named BotrAMP14 and CrotAMP14, which were re-designed from snake batroxicidin and crotalicidin, respectively. BotrAMP14 and CrotAMP14 showed broad-spectrum antibacterial activity against susceptible microorganisms and clinical isolates with minimal inhibitory concent...
Aim: Nitric oxide (NO) is an important mediator related to damage of the pulp tissue and at the s... more Aim: Nitric oxide (NO) is an important mediator related to damage of the pulp tissue and at the same time to regenerative pulp processes. However, it is not clear how common endodontic microorganisms can regulate this mediator. This study aimed to investigate NO production by macrophages and fibroblasts against Enterococcus faecalis- and Staphylococcus aureus-antigens. Methods: RAW 264.7 macrophages and L929 fibroblast cell lines were stimulated with different heat-killed (HK) antigen concentrations (105-108 colony forming units - CFU) from E. faecalis and S. aureus with or without interferon-gamma (IFN-γ). Cell viability by MTT colorimetric assay and NO production from the culture supernatants were evaluated after 72 h. Results: Data here reported demonstrated that none of the antigen concentrations decreased cell viability in macrophages and fibroblasts. The presence of HK-S. aureus and HK-E. faecalis antigen- stimulated NO production with or without IFN-γ on RAW 264.7. The HK-S. ...
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