Papers by Makoto Taketani
The Journal of Neuroscience, 2000
Regional variations and substrates of high-frequency rhythmic activity induced by cholinergic sti... more Regional variations and substrates of high-frequency rhythmic activity induced by cholinergic stimulation were studied in hippocampal slices with 64-electrode recording arrays. (1) Carbachol triggered β waves (17.6 ± 5.7 Hz) in pyramidal regions of 75% of the slices. (2) The waves had phase shifts across the cell body layers and were substantially larger in the apical dendrites than in cell body layers or basal dendrites. (3) Continuous, two-dimensional current source density analyses indicated apical sinks associated with basal sources, lasting ∼10 msec, followed by apical sources and basal sinks, lasting ∼20 msec, in a repeating pattern with a period in the range of 15–25 Hz. (4) Carbachol-induced β waves in the hippocampus were accompanied by 40 Hz (γ) oscillations in deep layers of the entorhinal cortex. (5) Cholinergically elicited β and γ rhythms were eliminated by antagonists of either AMPA or GABA receptors. Benzodiazepines markedly enhanced β activity and sometimes introduc...
NIPPON KAGAKU KAISHI, 1986
A method and apparatus for measuring a biological tissue or cells of the physical and chemical pr... more A method and apparatus for measuring a biological tissue or cells of the physical and chemical properties, the method can arbitrarily change the physical and chemical environment surrounding tissue or cells depending on the purpose of the experiment. The apparatus comprises a biological tissue or cells surrounding a constant means of maintaining (40); any means of changing the physical and chemical environment (50); means (10 and 20) determining the tissue or cell physicochemical properties; Comparative physical and chemical changes in the environment means tissue before and after physical or chemical properties of the cells (30). Monitoring means (10) is a potential measuring means for measuring electrophysiological properties of the tissue or cells, comprising the integrated cell placement device (1), which is provided with two or more microelectrodes (11) on a substrate, the cell is provided section (6) located thereon, for placing the cells or tissues described above, which further comprises a wiring pattern (pattern) (12), which provides an electrical signal to the microelectrodes (11) and deriving an electrical signal from the microelectrodes.
Advances in Network Electrophysiology
ABSTRACT Early research in the field of multi-electrode arrays (MEAs) was largely concerned with ... more ABSTRACT Early research in the field of multi-electrode arrays (MEAs) was largely concerned with the development of MEA hardware (see Chapter 1 for a review). This research lay the ground work that enabled commercial entities, such as Panasonic (Oka et al., 1999) and Multi Channel Systems (Egert et al., 1998), to develop and manufacture the first MEA-based instruments to be sold in large numbers. Current estimates place the number of instruments sold at over 250 worldwide. The proliferation of these instruments within the electrophysiology community has led to a variety of new applications. This chapter presents a sampling of these applications meant to outline some of the major application categories currently associated with MEAs. In what follows, MEA applications are divided into two broad categories: experiments that can be performed with traditional non-MEA instrumentation but are enhanced by the use of MEAs; and experiments that can only be performed using MEAs because they depend upon one or more of their unique characteristics. Within each of these broad categories a variety of protocols, tissue types, tissue preparations, and measurements is presented. The examples are largely drawn from users of the Panasonic MED64 System due to the authors unique access to these researchers; however, the broad outline offered here should apply to users of other MEA instrumentation as well. © 2006 Springer Science+Business Media, Inc. All rights reserved.
Neural Representation of Temporal Patterns, 1995
The kind of learning you are doing now is characterized by rapid acquisition (the speed of readin... more The kind of learning you are doing now is characterized by rapid acquisition (the speed of reading), enormous capacity (further learning does not force out or interfere with prior learning) and persistence: what you learn from a page can potentially last decades. These features are extremely unusual for any biological mechanism, for which homeostasis is the norm. The only known mechanism having these properties is synaptic long-term potentiation, or LTP, and it is therefore the leading, and currently the only, candidate mechanism for rapid, persistent, high-capacity learning. In contrast, slowly accreted learning of the kind that occurs when one learns motor skills such as riding a bicycle or playing racquetball, may be subserved by a different set of mechanisms.
Agricultural and Biological Chemistry, 1983
Protein synthesis in prokaryotes is initiated with formylated methionyl r-RNA. The amino-terminal... more Protein synthesis in prokaryotes is initiated with formylated methionyl r-RNA. The amino-terminal formyl residue, and often also amino-terminal methionyl residue, is removed from the finished polypeptide. Wehave previously reported that an amino-terminally formylated
Advances in Network Electrophysiology
Neural Plasticity, 2002
Long-term potentiation (LTP) was elicited by high frequency stimulation in hippocampal slices cul... more Long-term potentiation (LTP) was elicited by high frequency stimulation in hippocampal slices cultured on multi-electrode arrays. LTP lasting more than 1 h was recorded in 75% of slices, and a significant number of slices exhibited a non-decaying LTP that lasted more than 48 h. LTP induction was completely and reversibly blocked by an antagonist of the NMDA receptor, APV. Our results suggest the possibility of using chronic recording in hippocampal slices cultured on multi-electrode arrays to study the mechanisms underlying LTP maintenance and stabilization.
Continuous current source densities were calculated in two dimensions (proximo-distal vs. medio-l... more Continuous current source densities were calculated in two dimensions (proximo-distal vs. medio-lateral) from slices of hippocampal field CA1 placed on a 64-electrode array in the presence of GABA blockers. The synaptic sink generated by stimulation of the Schaffer-commissural fibers spread across the extent of field CA1 within the same sublamina of the stratum radiatum as the stimulation electrode. The size
Synthetic Metals, 1984
Fourier transform infrared (FTIR) spectra of electrochemically prepared polythienylene films dope... more Fourier transform infrared (FTIR) spectra of electrochemically prepared polythienylene films doped with various dopant species at varying doping levels have been obtained. These doped polythienylene films exhibit four conspicuous absorption bands at 1330 -1310, 1200, 1120 -1080 and 1030 -1020 cm-' made IR active and/or given prominent intensity due to the doping, independent of the difference in dopant species and contents. On the other hand, the bands specific to each dopant species are distinctly observable for the heavily-doped polythienylene films. Some typical band profiles in accordance with varied doping levels will be presented.
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Papers by Makoto Taketani