Background: Tumor-infiltrating CD8+ T cells are correlated with prolonged progression-free and ov... more Background: Tumor-infiltrating CD8+ T cells are correlated with prolonged progression-free and overall survival in epithelial ovarian cancer (EOC). A significant fraction of EOC patients mount autoantibody responses to various tumor antigens, however the relationship between autoantibodies and tumor-infiltrating T cells has not been investigated in EOC or any other human cancer. We hypothesized that autoantibody and T cell responses may be correlated in EOC and directed toward the same antigens.
Background: The classification of ancient animal corpses at the species level remains a challengi... more Background: The classification of ancient animal corpses at the species level remains a challenging task for forensic scientists and anthropologists. Severe damage and mixed, tiny pieces originating from several skeletons may render morphological classification virtually impossible. Standard approaches are based on sequencing mitochondrial and nuclear targets.
The article argues that many forms of diversity instruction, be it in the classroom or a conferen... more The article argues that many forms of diversity instruction, be it in the classroom or a conference room, have promoted an appreciation of differences without simultaneously promoting inclusion. Therefore, we advocate that diversity instruction should take a strategic approach to cultivating an appreciation of inclusion as well as diversity through considering the structure of the instruction, the authors of the
Background: Historical records suggest that multiple burial sites from the 14th-16 th centuries i... more Background: Historical records suggest that multiple burial sites from the 14th-16 th centuries in Venice, Italy, were used during the Black Death and subsequent plague epidemics.
It has been previously established that trans-10, cis-12 CLA is a potent inhibitor of milk fat sy... more It has been previously established that trans-10, cis-12 CLA is a potent inhibitor of milk fat synthesis. Although the mechanism of this action is not completely understood, it has been speculated that eicosanoid-like metabolites of this isomer formed by the activity of tissue desaturases may be responsible for its activity. The objective of this study was to investigate the effects of an enrichment containing an 18∶3 conjugated diene, produced in the metabolism of trans-10, cis-12 CLA, on milk fat synthesis. Three rumen-fistulated Holstein cows (210±8 d in milk) were randomly assigned in a 3×3 Latin square experiment. Treatments were (i) control, (ii) trans-10, cis-12 CLA supplement (2.1 g/d; positive control), (iii) enrichment providing two conjugated diene 18∶3 isomers (2.6 g/d of cis-6, trans-10, cis-12 and 4.0 g/d of cis-6, trans-8, cis-12) and trans-10, cis-12 CLA (2.1 g/d). Treatments were abomasally infused for 5 d at 4-h intervals, and there was a 7-d interval between periods. Milk yield, dry matter intake, and milk protein yield were unaffected by treatments. In contrast, the trans-10, cis-12 CLA supplement reduced milk fat yield by 27%, whereas the supplement enriched with conjugated diene 18∶3 isomers (treatment iii) had no effect on milk fat yield beyond that attributable to its trans-10, cis-12 CLA content. The transfer efficiency of trans-10, cis-12 CLA into milk fat was 25 and 24% for treatments ii and iii, respectively. At the same time, the abomasally infused conjugated diene 18∶3 isomers were transferred to milk fat with an efficiency of 33 and 41% for cis-6, trans-10, cis-12 and cis-6, trans-8, cis-12 18∶3, respectively. Overall, short-term abomasal infusion of the conjugated diene 18∶3 isomers had no effect on milk fat synthesis, thereby offering no support for an involvement of metabolies of trans-10, cis-12 CLA in the regulation of milk fat synthesis.
Background. Dental pulp is used for PCR-based detection of DNA derived from host and bacteremic m... more Background. Dental pulp is used for PCR-based detection of DNA derived from host and bacteremic microorganims. Current protocols require odontology expertise for proper recovery of the dental pulp. Dental pulp specimen exposed to laboratory environment yields contaminants detected using universal 16S rDNA-based detection of bacteria. Methodology/Principal Findings. We developed a new protocol by encasing decontaminated tooth into sterile resin, extracting DNA into the dental pulp chamber itself and decontaminating PCR reagents by filtration and double restriction enzyme digestion. Application to 16S rDNA-based detection of bacteria in 144 teeth collected in 86 healthy people yielded a unique sequence in only 14 teeth (9.7%) from 12 individuals (14%). Each individual yielded a unique 16S rDNA sequence in 1-2 teeth per individual. Negative controls remained negative. Bacterial identifications were all confirmed by amplification and sequencing of specific rpoB sequence. Conclusions/Significance. The new protocol prevented laboratory contamination of the dental pulp. It allowed the detection of bacteria responsible for dental pulp colonization from blood and periodontal tissue. Only 10% such samples contained 16S rDNA. It provides a new tool for the retrospective diagnostic of bacteremia by allowing the universal detection of bacterial DNA in animal and human, contemporary or ancient tooth. It could be further applied to identification of host DNA in forensic medicine and anthropology.
... control and self-management. Together with Pablo Ghigliani, he is project coordinator of Labo... more ... control and self-management. Together with Pablo Ghigliani, he is project coordinator of LabourAgain (www.iisg.nl/labouragain), a research network on labour and social mobilisation in Latin America. Ernst Jünger On Pain, trans. ...
Background: The cause of past plague pandemics was controversial but several research teams used ... more Background: The cause of past plague pandemics was controversial but several research teams used PCR techniques and dental pulp as the primary material to reveal that they were caused by Yersinia pestis. However, the degradation of DNA limits the ability to detect ancient infections.
Background. Dental pulp is used for PCR-based detection of DNA derived from host and bacteremic m... more Background. Dental pulp is used for PCR-based detection of DNA derived from host and bacteremic microorganims. Current protocols require odontology expertise for proper recovery of the dental pulp. Dental pulp specimen exposed to laboratory environment yields contaminants detected using universal 16S rDNA-based detection of bacteria. Methodology/Principal Findings. We developed a new protocol by encasing decontaminated tooth into sterile resin, extracting DNA into the dental pulp chamber itself and decontaminating PCR reagents by filtration and double restriction enzyme digestion. Application to 16S rDNA-based detection of bacteria in 144 teeth collected in 86 healthy people yielded a unique sequence in only 14 teeth (9.7%) from 12 individuals (14%). Each individual yielded a unique 16S rDNA sequence in 1-2 teeth per individual. Negative controls remained negative. Bacterial identifications were all confirmed by amplification and sequencing of specific rpoB sequence. Conclusions/Significance. The new protocol prevented laboratory contamination of the dental pulp. It allowed the detection of bacteria responsible for dental pulp colonization from blood and periodontal tissue. Only 10% such samples contained 16S rDNA. It provides a new tool for the retrospective diagnostic of bacteremia by allowing the universal detection of bacterial DNA in animal and human, contemporary or ancient tooth. It could be further applied to identification of host DNA in forensic medicine and anthropology.
Appropriate food of suitable nutritional value is crucial for first-feeding stages of the larvae ... more Appropriate food of suitable nutritional value is crucial for first-feeding stages of the larvae of cobia Rachycentron canadum, a very fast growing marine fish species. Best survival and growth results in cobia larviculture have been reported with a starter diet of HUFA-enriched rotifers and -as mouth size permitsfollowed by freshly-hatched and eventually HUFA-enriched Artemia nauplii. Using the smaller-sized Vietnam Artemia franciscana (AF) strain instead of the Great Salt Lake A. franciscana strain, it has been shown that the rotifer-feeding period could be shortened with 3 days, resulting in significant improvements in larval survival and growth. This study verified the possibility to feed umbrella-stage Artemia for further shortening and eventually completely substituting rotifer start feeding. The experiment was conducted in 200-L tanks and lasted 18 days. AF umbrella Artemia was used as sole feed during the whole rotifer feeding period (UAF), compared to the use of enriched rotifers for the first 2 days followed by AFumbrella (ER + UAF) and the use of enriched rotifers as control (ER). The feeding incidence of UAF treatments was significantly lower (P b 0.05) in the 1st feeding day, however, the ingestion and digestion of AF were evident. Growth and survival as well as deformities at day 18 post-hatching were not significantly different for all treatments (PN 0.05). The viability of cobia larvae after exposure to high salinity stress was lower in the ER treatment at day 8 post-hatching, but higher at day 18 post-hatching (Pb 0.05). The ability of cobia larvae to ingest and digest AF umbrella at first feeding as well as the nutritional suitability of AF umbrella is discussed. The possibility to use umbrella-stage Artemia opens an opportunity to simplify the rearing protocol and to reduce production costs of cobia larviculture.
Background: Tumor-infiltrating CD8+ T cells are correlated with prolonged progression-free and ov... more Background: Tumor-infiltrating CD8+ T cells are correlated with prolonged progression-free and overall survival in epithelial ovarian cancer (EOC). A significant fraction of EOC patients mount autoantibody responses to various tumor antigens, however the relationship between autoantibodies and tumor-infiltrating T cells has not been investigated in EOC or any other human cancer. We hypothesized that autoantibody and T cell responses may be correlated in EOC and directed toward the same antigens.
Background: The classification of ancient animal corpses at the species level remains a challengi... more Background: The classification of ancient animal corpses at the species level remains a challenging task for forensic scientists and anthropologists. Severe damage and mixed, tiny pieces originating from several skeletons may render morphological classification virtually impossible. Standard approaches are based on sequencing mitochondrial and nuclear targets.
The article argues that many forms of diversity instruction, be it in the classroom or a conferen... more The article argues that many forms of diversity instruction, be it in the classroom or a conference room, have promoted an appreciation of differences without simultaneously promoting inclusion. Therefore, we advocate that diversity instruction should take a strategic approach to cultivating an appreciation of inclusion as well as diversity through considering the structure of the instruction, the authors of the
Background: Historical records suggest that multiple burial sites from the 14th-16 th centuries i... more Background: Historical records suggest that multiple burial sites from the 14th-16 th centuries in Venice, Italy, were used during the Black Death and subsequent plague epidemics.
It has been previously established that trans-10, cis-12 CLA is a potent inhibitor of milk fat sy... more It has been previously established that trans-10, cis-12 CLA is a potent inhibitor of milk fat synthesis. Although the mechanism of this action is not completely understood, it has been speculated that eicosanoid-like metabolites of this isomer formed by the activity of tissue desaturases may be responsible for its activity. The objective of this study was to investigate the effects of an enrichment containing an 18∶3 conjugated diene, produced in the metabolism of trans-10, cis-12 CLA, on milk fat synthesis. Three rumen-fistulated Holstein cows (210±8 d in milk) were randomly assigned in a 3×3 Latin square experiment. Treatments were (i) control, (ii) trans-10, cis-12 CLA supplement (2.1 g/d; positive control), (iii) enrichment providing two conjugated diene 18∶3 isomers (2.6 g/d of cis-6, trans-10, cis-12 and 4.0 g/d of cis-6, trans-8, cis-12) and trans-10, cis-12 CLA (2.1 g/d). Treatments were abomasally infused for 5 d at 4-h intervals, and there was a 7-d interval between periods. Milk yield, dry matter intake, and milk protein yield were unaffected by treatments. In contrast, the trans-10, cis-12 CLA supplement reduced milk fat yield by 27%, whereas the supplement enriched with conjugated diene 18∶3 isomers (treatment iii) had no effect on milk fat yield beyond that attributable to its trans-10, cis-12 CLA content. The transfer efficiency of trans-10, cis-12 CLA into milk fat was 25 and 24% for treatments ii and iii, respectively. At the same time, the abomasally infused conjugated diene 18∶3 isomers were transferred to milk fat with an efficiency of 33 and 41% for cis-6, trans-10, cis-12 and cis-6, trans-8, cis-12 18∶3, respectively. Overall, short-term abomasal infusion of the conjugated diene 18∶3 isomers had no effect on milk fat synthesis, thereby offering no support for an involvement of metabolies of trans-10, cis-12 CLA in the regulation of milk fat synthesis.
Background. Dental pulp is used for PCR-based detection of DNA derived from host and bacteremic m... more Background. Dental pulp is used for PCR-based detection of DNA derived from host and bacteremic microorganims. Current protocols require odontology expertise for proper recovery of the dental pulp. Dental pulp specimen exposed to laboratory environment yields contaminants detected using universal 16S rDNA-based detection of bacteria. Methodology/Principal Findings. We developed a new protocol by encasing decontaminated tooth into sterile resin, extracting DNA into the dental pulp chamber itself and decontaminating PCR reagents by filtration and double restriction enzyme digestion. Application to 16S rDNA-based detection of bacteria in 144 teeth collected in 86 healthy people yielded a unique sequence in only 14 teeth (9.7%) from 12 individuals (14%). Each individual yielded a unique 16S rDNA sequence in 1-2 teeth per individual. Negative controls remained negative. Bacterial identifications were all confirmed by amplification and sequencing of specific rpoB sequence. Conclusions/Significance. The new protocol prevented laboratory contamination of the dental pulp. It allowed the detection of bacteria responsible for dental pulp colonization from blood and periodontal tissue. Only 10% such samples contained 16S rDNA. It provides a new tool for the retrospective diagnostic of bacteremia by allowing the universal detection of bacterial DNA in animal and human, contemporary or ancient tooth. It could be further applied to identification of host DNA in forensic medicine and anthropology.
... control and self-management. Together with Pablo Ghigliani, he is project coordinator of Labo... more ... control and self-management. Together with Pablo Ghigliani, he is project coordinator of LabourAgain (www.iisg.nl/labouragain), a research network on labour and social mobilisation in Latin America. Ernst Jünger On Pain, trans. ...
Background: The cause of past plague pandemics was controversial but several research teams used ... more Background: The cause of past plague pandemics was controversial but several research teams used PCR techniques and dental pulp as the primary material to reveal that they were caused by Yersinia pestis. However, the degradation of DNA limits the ability to detect ancient infections.
Background. Dental pulp is used for PCR-based detection of DNA derived from host and bacteremic m... more Background. Dental pulp is used for PCR-based detection of DNA derived from host and bacteremic microorganims. Current protocols require odontology expertise for proper recovery of the dental pulp. Dental pulp specimen exposed to laboratory environment yields contaminants detected using universal 16S rDNA-based detection of bacteria. Methodology/Principal Findings. We developed a new protocol by encasing decontaminated tooth into sterile resin, extracting DNA into the dental pulp chamber itself and decontaminating PCR reagents by filtration and double restriction enzyme digestion. Application to 16S rDNA-based detection of bacteria in 144 teeth collected in 86 healthy people yielded a unique sequence in only 14 teeth (9.7%) from 12 individuals (14%). Each individual yielded a unique 16S rDNA sequence in 1-2 teeth per individual. Negative controls remained negative. Bacterial identifications were all confirmed by amplification and sequencing of specific rpoB sequence. Conclusions/Significance. The new protocol prevented laboratory contamination of the dental pulp. It allowed the detection of bacteria responsible for dental pulp colonization from blood and periodontal tissue. Only 10% such samples contained 16S rDNA. It provides a new tool for the retrospective diagnostic of bacteremia by allowing the universal detection of bacterial DNA in animal and human, contemporary or ancient tooth. It could be further applied to identification of host DNA in forensic medicine and anthropology.
Appropriate food of suitable nutritional value is crucial for first-feeding stages of the larvae ... more Appropriate food of suitable nutritional value is crucial for first-feeding stages of the larvae of cobia Rachycentron canadum, a very fast growing marine fish species. Best survival and growth results in cobia larviculture have been reported with a starter diet of HUFA-enriched rotifers and -as mouth size permitsfollowed by freshly-hatched and eventually HUFA-enriched Artemia nauplii. Using the smaller-sized Vietnam Artemia franciscana (AF) strain instead of the Great Salt Lake A. franciscana strain, it has been shown that the rotifer-feeding period could be shortened with 3 days, resulting in significant improvements in larval survival and growth. This study verified the possibility to feed umbrella-stage Artemia for further shortening and eventually completely substituting rotifer start feeding. The experiment was conducted in 200-L tanks and lasted 18 days. AF umbrella Artemia was used as sole feed during the whole rotifer feeding period (UAF), compared to the use of enriched rotifers for the first 2 days followed by AFumbrella (ER + UAF) and the use of enriched rotifers as control (ER). The feeding incidence of UAF treatments was significantly lower (P b 0.05) in the 1st feeding day, however, the ingestion and digestion of AF were evident. Growth and survival as well as deformities at day 18 post-hatching were not significantly different for all treatments (PN 0.05). The viability of cobia larvae after exposure to high salinity stress was lower in the ER treatment at day 8 post-hatching, but higher at day 18 post-hatching (Pb 0.05). The ability of cobia larvae to ingest and digest AF umbrella at first feeding as well as the nutritional suitability of AF umbrella is discussed. The possibility to use umbrella-stage Artemia opens an opportunity to simplify the rearing protocol and to reduce production costs of cobia larviculture.
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