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Rogerio Vaz

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PhD in Bioprocess Engineering and Biotechnology, Federal University of Parana (CONCEPT 07 - CAPES). (PECS - Area: Health Education / Internationalization of Brazilian HEIs), Lecturer (undergraduate courses):Biomedicine / Pharmacy and Medicine (Basic and Clinical Immunology, Molecular Genetics). Experience in : Basic and Clinical Immunology and in Genetic-Molecular Sciences, working mainly in the following subjects: biotechnology, genomics and proteomics of organisms of clinical and bioindustrial interest, administration of R

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The University of Sheffield

Kansas State University

University of California, Berkeley

Edith Cowan University

University of Oregon

Università degli Studi di Bergamo (University of Bergamo)

Imam Damar Djati

Bandung Institute of Technology

Jorge Luis Gonzalez

University of Cambridge

Samson Chukwuedo

University of Benin, Benin City , Nigeria.

Delta university for science and technology

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Papers by Rogerio Vaz

Sarcoma De Kaposi Em Crianças Vivendo Com Hiv: Uma Revisão Integrativa Sobre Os Sintomas e Fisiopatologia

Medicina: Longe dos holofotes, perto das pessoas, 2021

Transplante de cÃ©lulas tronco hematopoiÃ©ticas alogÃªnico e doenÃ§a do enxerto contra o hospedeiro aguda (DECHa): uma revisÃ£o da profilaxia e tratamento

Brazilian Journal Allergy and Immunology, 2009

Congenital Toxoplasmosis: A Neglected Disease? – Current Brazilian public health policy

Toxoplasmosis is a cosmopolite disease caused by the protozoan parasite, Toxoplasma gondii. The i... more Toxoplasmosis is a cosmopolite disease caused by the protozoan parasite, Toxoplasma gondii. The infection may be contracted through the ingestion of raw or undercooked meat or unpasteurised milk, organ transplants, blood transfusion, through the placenta in vertical transmission or by direct contact with the faeces of infected felids or even through the ingestion of sporulated oocysts in water or food. In immunocompetent individuals, it is generally asymptomatic. However, in patients with various degrees of immunodeficiency and in pregnant women, it may cause severe sequelae and can be fatal. In pregnant women, the prenatal diagnosis should be made as early as possible, so that therapy may be applied to reduce parasitemia and avoid transplacental infection. Despite the high prevalence of infected individuals around the world (20 - 90%), in some European Union countries, such as France and Austria, the average incidence of foetal toxoplasmosis was reduced from 40% to 7% by means of s...

Webinar on the perception of Brazilian, Italian, Swiss, and French medical science undergraduates about the COVID-19 pandemic: Experience Report

MedEdPublish

The Covid-19 pandemic highlighted the need for Global Health (GH) understanding between medical s... more The Covid-19 pandemic highlighted the need for Global Health (GH) understanding between medical science undergraduates and the need for new online teaching methods during social isolation. This paper aims to report the experience of an international webinar on the perception of medical science undergraduates from Brazil, Italy, France, and Switzerland about the Covid-19 pandemic. This activity allowed students comprehension of different realities faced by other countries, education on global epidemiology, comparison government and university maneuvers to handle the new coronavirus disease, and exchange of experiences among medical undergraduates concerning the ways of maintaining their mental well-being. Thereupon, it is necessary to implement GH activities within the medical curricula to promote a qualified education that addresses the needs of the digital and globalized world.

O Acometimento Pulmonar Da Coinfecção De COVID-19 Com Tuberculose: Uma Revisão Integrativa Da Literatura

Anais do I Congresso Brasileiro de Doenças Infectocontagiosas On-line

Introdução: A tuberculose é uma infecção causada pela bactéria Mycobacterium tuberculosis, acomet... more Introdução: A tuberculose é uma infecção causada pela bactéria Mycobacterium tuberculosis, acometendo mais frequentemente os pulmões. Já a COVID-19 consiste em uma infecção respiratória aguda ocasionada pelo SARS-CoV-2, um RNA vírus envelopado. Além disso, ambas as doenças apresentam semelhanças clínicas e radiológicas e podem levar a óbito. Objetivo: Tal estudo objetiva relacionar aspectos da coinfecção de tuberculose e COVID-19. Material e métodos: Trata-se de uma revisão integrativa da literatura, em que se utilizou os descritores “Coinfection”, “COVID-19” e “Mycobacterium tuberculosis” para busca nas bases de dados Pubmed, Biblioteca Virtual em Saúde e Portal de Periódicos CAPES. Foram selecionados artigos publicados nos últimos 5 anos, em inglês, português e espanhol. Resultados: Embora a infecção por tuberculose apresente-se cronicamente a infecção por COVID-19 de forma aguda, um obstáculo no diagnóstico dos casos de coinfecção de tais doenças consiste na mimetização dos sinto...

The role of natural killer cells in the uterine microenvironment during pregnancy

Cellular & molecular immunology, Jan 23, 2018

Transplante de células tronco hematopoiéticas alogênico e doença do enxerto contra o hospedeiro aguda (DECHa): uma revisão da profilaxia e tratamento

Rev Bras Alergia Imunopatol, Dec 1, 2009

Molecular Characterization of Toxoplasma gondii Strains Isolated from Pregnant Women Treated with Antiparasitic Protocol Drugs Attended by the Public Health Services in the City of Curitiby – Southern Brazil

Nature Genetics, Jan 17, 2008

Toxoplasma gondii belongs to the phylum Apicomplexa, a group of obligate intracellular parasites ... more Toxoplasma gondii belongs to the phylum Apicomplexa, a group of obligate intracellular parasites that rely on gliding motility to enter host cells. Drugs interfering with the actin cytoskeleton block parasite motility, host cell invasion, and egress from infected cells. Myosin A, profilin, formin 1, formin 2, and actin-depolymerizing factor have all been implicated in parasite motility, yet little is known regarding the importance of actin polymerization and other myosins for the remaining steps of the parasite lytic cycle. Here we establish that T. gondii formin 3 (TgFRM3), a newly described formin homology 2 domain (FH2)-containing protein, binds to Toxoplasma actin and nucleates rabbit actin assembly in vitro. TgFRM3 expressed as a transgene exhibits a patchy localization at several distinct structures within the parasite. Disruption of the TgFRM3 gene by double homologous recombination in a ku80-ko strain reveals no vital function for tachyzoite propagation in vitro, which is consistent with its weak level of expression in this life stage. Conditional stabilization of truncated forms of TgFRM3 suggests that different regions of the molecule contribute to distinct localizations. Moreover, expression of TgFRM3 lacking the C-terminal domain severely affects parasite growth and replication. This work provides a first insight into how this specialized formin, restricted to the group of coccidia, completes its actin-nucleating activity.

In Vivo and In Vitro Isolation of Toxoplasma gondii Resistant Strains from Pregnant Women with Clinical and Serological Indications of Acute Toxoplasmosis

Transfusion-Transmitted Pathogens: Evaluation of Anti-Toxoplasma Gondii Antibodies Seroprevalence Among Volunteer Blood Donors Associated to Transfusional Risks at the Official Hemotherapy Center and the Public Health Systems in Curitiba City, Parana, Southern of Brazil

European Journal of Immunology, Sep 13, 2009

Optimum aerodynamic design for wind turbine blade with a Rankine vortex wake

Renewable Energy, 2013

ABSTRACT This paper presents a model to optimize the distribution of chord and twist angle of hor... more ABSTRACT This paper presents a model to optimize the distribution of chord and twist angle of horizontal axis wind turbine blades, taking into account the influence of the wake, by using a Rankine vortex. This model is applied to both large and small wind turbines, aiming to improve the aerodynamics of the wind rotor, and particularly useful for the case of wind turbines operating at low tip-speed ratios. The proposed optimization is based on maximizing the power coefficient, coupled with the general relationship between the axial induction factor in the rotor plane and in the wake. The results show an increase in the chord and a slightly decrease in the twist angle distributions as compared to other classical optimization methods, resulting in an improved aerodynamic shape of the blade. An evaluation of the efficiency of wind rotors designed with the proposed model is developed and compared other optimization models in the literature, showing an improvement in the power coefficient of the wind turbine. Published by Elsevier Ltd.

III – FQRNT Centre for Host-Parasite Interactions Centre de recherche sur les interactions hôte-parasite McGill University - Montreal - Canada

III – FQRNT Centre for Host-Parasite Interactions Centre de recherche sur les interactions hôte-p... more III – FQRNT Centre for Host-Parasite Interactions Centre de recherche sur les interactions hôte-parasite In 2006-2007, our Provincially-funded Regroupement Stratégique Centre completed its fourth busy year of operation. Please visit the website www.mcgill.ca/chpi for details of our scientific activities, and services offered, throughout the Centre. The Centre for Host-Parasite Interactions brings together researchers and their trainees at the Institute of Parasitology, Department of Microbiology and Immunology, Department of Biology, Department of Chemistry, Department of Epidemiology and Biostatistics, Department of Physiology, Department of Chemical Engineering, Department of Bioresource Engineering, School of Dietetics and Human Nutrition, Brace Research Institute, McGill Centre for Tropical Medicine and the McGill University Health Centre, at McGill University, Université de Montréal, Centre de recherche en Infectiologie, Université Laval, INRS – Institut Armand-Frappier, and Un...

Good Quality Scintigraphic Images in Patients with Low 99mTc-HMPAO Leucocyte Labelling Efficiency

Immunological and Molecular Factors Involved on Hard Tissue Resorption int Periodontal Disease

Immunological and Molecular Factors Involved on Hard Tissue Resorption in the Periodontal Disease... more Immunological and Molecular Factors Involved on Hard Tissue Resorption in the Periodontal Disease anisms and causes involved in the hard tissue resorption in periodontal focal sites. This disease is widely disseminated through our population, and thus require the comprehension of its immunophysiopathological mechanisms, in order to improve and develop preventive measures and treatment. Within periodontal sites is located the dentoalveolar articulation, rich in cells, fibers, microcirculation vases and nerves, and it is in this microenvironment that tissue changes may occur in response to the Innate and Acquired Immune System activation. The activation of specific cells induced by cytokines, growth factors, proteins and hormones seems to be the main remodulation mechanism of hard tissue. Neverthe- less, the cell to cell contact during the activation of the resorp- tion process, in the presence or absence of LPS producing bac- teria, could use receptor activator NF-kB (RANK), and its ...

Origem e Tipos de Fagócitos. Phagocytes: Types & Origin

Os fagócitos são componentes celulares da imunidade inata ou natural responsáveis pela primeira l... more Os fagócitos são componentes celulares da imunidade inata ou natural responsáveis pela primeira linha de defesa do organismo contra infecções, eliminando os microrganismos invasores (patógenos) através de um mecanismo efetor denominado fagocitose. O presente estudo apresenta uma revisão sobre os tipos de células fagocíticas, bem como sobre sua origem e maturação. Palavras-chave: fagócitos, granulócitos polimorfonucleares, neutrófilos, monócitos e macrófagos.

Biotecnologia na Indústria Farmacêutica

A indústria farmacêutica necessita de processos biotecnológicos para obtenção de vários produtos ... more A indústria farmacêutica necessita de processos biotecnológicos para obtenção de vários produtos importantes para a saúde humana e animal. A história da biotecnologia moderna começa inclusive com o desenvolvimento de um medicamento, a penicilina, na primeira metade do século XX. A partir de então, processos biotecnológicos são utilizados na produ ção de vitaminas, hormônios, antibióticos, vacinas e enzimas. Este trabalho apresenta as características gerais desses processos.

Depressão / Evidências Genético-Moleculares De Vulnerabilidade a Distúrbios / Doenças Afetivas

Rogério Saad Vaz1 ,BMD,PhD 1-Biomédico, Pós-grad. em Imunologia pela Universidade Livre de Bruxel... more Rogério Saad Vaz1 ,BMD,PhD 1-Biomédico, Pós-grad. em Imunologia pela Universidade Livre de Bruxelas e Univ. Estadualde Gent - Bélgica, PhD em Engenharia de Bioprocessos e Biotecnologia pela UFPR (Área - Saúde Humana e Animal) - UFPR rogeriovaz@fpp.edu.br DEPRESSÃO: EVIDÊNCIAS GENÉTICO-MOLECULARES DE VULNERABILIDADE A DISTÚRBIOS E DOENÇAS AFETIVAS. Em estudos de base genética humana e clínica relacionados a doenças afetivas,o transtorno afetivo bipolar tem sido o mais freqüentemente estudado, em função da relação de herdabilidade deste transtorno. Localização - Cromossômica A região de maior probabilidade de relação com doença bipolar está localizada no braço longo do cromossomo X (Xq28).Winokur et al. (1969) e Mendlewicz et al. (1974) demonstraram uma cosegregação da doença maníaco-depressiva com a cegueira para cores. MendlewicZ et al. (1980) pareceram confirmar este achado inicial, observando uma ligação entre a deficiência da enzima glicose-6-fosfato desidrogenase (G-6-PD) com di...

HTLV-I/II Detection by Low Cost PCR of Diluted and Pooled Blood Samples

JAIDS Journal of Acquired Immune Deficiency Syndromes

The aim of this study was to evaluate HTLV I/II sensitivity of pooled and serial diluted samples ... more The aim of this study was to evaluate HTLV I/II sensitivity of pooled and serial diluted samples by PCR (polumerase Chain Reaction). This could provide the possibility of epidemilogical studies using this methodology. Samples of 4 HTLV I (+) (ELISA/WB) individuals confirmed by PCR (Roche Molecular Sustems KIT) were analysed. The samples (whole blood/ pelleted leukocytes) were diluted and amplified by PCR, and the products were detected by Enzyme Immune Assay (specific probes). The results were calculated according to OD/CO values. The established cut off was 0.250. All samples were also tested for HTLV II and resulted negative. All samples (100%) were positive to the dilution of 1/50, 3 were positive (37.5%) and 5 were negative (62.5%) for the dilution 1/100. We concluded that the dilution to 1/50 of pooled samples seemend to be efficient. In comparison with serological tests, PCR of pooled samples is by itself a confirming tes. Further studies on PCR diluted samples have to be done...

1.Toxoplasmosis & Pregnacy – Curitiba (Parana -Southern of Brazil). 2. Transfusions Risks and Toxoplasmosis III – FQRNT Centre for Host-Parasite Interactions Centre de recherche sur les interactions hôte-parasite (McGill Univ. Montreal Canada)

The Centre for Host-Parasite Interactions brings together researchers and their trainees at the I... more The Centre for Host-Parasite Interactions brings together researchers and their trainees at the Institute of Parasitology, Department of Microbiology and Immunology, Department of Biology, Department of Chemistry, Department of Epidemiology and Biostatistics, Department of Physiology, Department of Chemical Engineering, Department of Bioresource Engineering, School of Dietetics and Human Nutrition, Brace Research Institute, McGill Centre for Tropical Medicine and the McGill University Health Centre, at McGill University, Université de Montréal, Centre de recherche en Infectiologie, Université Laval, INRS – Institut Armand-Frappier, and Université du Québec à Montréal into what is considered by external reviewers as the strongest centre for parasitological research in the world. It is recognized as an FQRNT Regroupement stratégique. The research of the Centre focuses on Mechanisms of Pathogenesis and Host Defense to Parasite Infections, and the Molecular Basis of Anti-parasite Drug R...

Technical Requirement for Implementation of Sorological Tests for the Detection of Toxoplasma Gondii in Routinely Services of Hemotherapy with Voluntary Blood Donators of the Official Net in Curitiba City, Paraná, Southern of Brazil

ABSTRACT Serological assays to detect anti-Toxoplasma gondii antibodies and specific anamnesis as... more ABSTRACT Serological assays to detect anti-Toxoplasma gondii antibodies and specific anamnesis associated with this highly prevalent infection could be necessary in hemotherapy services in order to regulate safer blood supplies thus avoiding unnecessary transfusion risks. Even though, such policies are not yet implemented. In order to evaluate this requirement, our research group designed a questionnaire filled up by 132 volunteer blood donors from Parana Hemotherapy Center (Hemepar) assessing the possible risk factors to toxoplasmic infection. A total of 20 IgG anti-Toxoplasma serological tests were done to check out for positive-reactivity. 60% of the selected serum samples reacted positively; from these, 50% reported having pets (average of 2 animals per person) and 33% of these pets were "semi-free". It suggested the probability of toxoplasmic infection through these animals. Only 2.2% of the interviewed individuals were aware of the correct concept of the disease and ...

Sarcoma De Kaposi Em Crianças Vivendo Com Hiv: Uma Revisão Integrativa Sobre Os Sintomas e Fisiopatologia

Medicina: Longe dos holofotes, perto das pessoas, 2021

Transplante de cÃ©lulas tronco hematopoiÃ©ticas alogÃªnico e doenÃ§a do enxerto contra o hospedeiro aguda (DECHa): uma revisÃ£o da profilaxia e tratamento

Brazilian Journal Allergy and Immunology, 2009

Congenital Toxoplasmosis: A Neglected Disease? – Current Brazilian public health policy

Toxoplasmosis is a cosmopolite disease caused by the protozoan parasite, Toxoplasma gondii. The i... more Toxoplasmosis is a cosmopolite disease caused by the protozoan parasite, Toxoplasma gondii. The infection may be contracted through the ingestion of raw or undercooked meat or unpasteurised milk, organ transplants, blood transfusion, through the placenta in vertical transmission or by direct contact with the faeces of infected felids or even through the ingestion of sporulated oocysts in water or food. In immunocompetent individuals, it is generally asymptomatic. However, in patients with various degrees of immunodeficiency and in pregnant women, it may cause severe sequelae and can be fatal. In pregnant women, the prenatal diagnosis should be made as early as possible, so that therapy may be applied to reduce parasitemia and avoid transplacental infection. Despite the high prevalence of infected individuals around the world (20 - 90%), in some European Union countries, such as France and Austria, the average incidence of foetal toxoplasmosis was reduced from 40% to 7% by means of s...

Webinar on the perception of Brazilian, Italian, Swiss, and French medical science undergraduates about the COVID-19 pandemic: Experience Report

MedEdPublish

The Covid-19 pandemic highlighted the need for Global Health (GH) understanding between medical s... more The Covid-19 pandemic highlighted the need for Global Health (GH) understanding between medical science undergraduates and the need for new online teaching methods during social isolation. This paper aims to report the experience of an international webinar on the perception of medical science undergraduates from Brazil, Italy, France, and Switzerland about the Covid-19 pandemic. This activity allowed students comprehension of different realities faced by other countries, education on global epidemiology, comparison government and university maneuvers to handle the new coronavirus disease, and exchange of experiences among medical undergraduates concerning the ways of maintaining their mental well-being. Thereupon, it is necessary to implement GH activities within the medical curricula to promote a qualified education that addresses the needs of the digital and globalized world.

O Acometimento Pulmonar Da Coinfecção De COVID-19 Com Tuberculose: Uma Revisão Integrativa Da Literatura

Anais do I Congresso Brasileiro de Doenças Infectocontagiosas On-line

Introdução: A tuberculose é uma infecção causada pela bactéria Mycobacterium tuberculosis, acomet... more Introdução: A tuberculose é uma infecção causada pela bactéria Mycobacterium tuberculosis, acometendo mais frequentemente os pulmões. Já a COVID-19 consiste em uma infecção respiratória aguda ocasionada pelo SARS-CoV-2, um RNA vírus envelopado. Além disso, ambas as doenças apresentam semelhanças clínicas e radiológicas e podem levar a óbito. Objetivo: Tal estudo objetiva relacionar aspectos da coinfecção de tuberculose e COVID-19. Material e métodos: Trata-se de uma revisão integrativa da literatura, em que se utilizou os descritores “Coinfection”, “COVID-19” e “Mycobacterium tuberculosis” para busca nas bases de dados Pubmed, Biblioteca Virtual em Saúde e Portal de Periódicos CAPES. Foram selecionados artigos publicados nos últimos 5 anos, em inglês, português e espanhol. Resultados: Embora a infecção por tuberculose apresente-se cronicamente a infecção por COVID-19 de forma aguda, um obstáculo no diagnóstico dos casos de coinfecção de tais doenças consiste na mimetização dos sinto...

The role of natural killer cells in the uterine microenvironment during pregnancy

Cellular & molecular immunology, Jan 23, 2018

Transplante de células tronco hematopoiéticas alogênico e doença do enxerto contra o hospedeiro aguda (DECHa): uma revisão da profilaxia e tratamento

Rev Bras Alergia Imunopatol, Dec 1, 2009

Molecular Characterization of Toxoplasma gondii Strains Isolated from Pregnant Women Treated with Antiparasitic Protocol Drugs Attended by the Public Health Services in the City of Curitiby – Southern Brazil

Nature Genetics, Jan 17, 2008

Toxoplasma gondii belongs to the phylum Apicomplexa, a group of obligate intracellular parasites ... more Toxoplasma gondii belongs to the phylum Apicomplexa, a group of obligate intracellular parasites that rely on gliding motility to enter host cells. Drugs interfering with the actin cytoskeleton block parasite motility, host cell invasion, and egress from infected cells. Myosin A, profilin, formin 1, formin 2, and actin-depolymerizing factor have all been implicated in parasite motility, yet little is known regarding the importance of actin polymerization and other myosins for the remaining steps of the parasite lytic cycle. Here we establish that T. gondii formin 3 (TgFRM3), a newly described formin homology 2 domain (FH2)-containing protein, binds to Toxoplasma actin and nucleates rabbit actin assembly in vitro. TgFRM3 expressed as a transgene exhibits a patchy localization at several distinct structures within the parasite. Disruption of the TgFRM3 gene by double homologous recombination in a ku80-ko strain reveals no vital function for tachyzoite propagation in vitro, which is consistent with its weak level of expression in this life stage. Conditional stabilization of truncated forms of TgFRM3 suggests that different regions of the molecule contribute to distinct localizations. Moreover, expression of TgFRM3 lacking the C-terminal domain severely affects parasite growth and replication. This work provides a first insight into how this specialized formin, restricted to the group of coccidia, completes its actin-nucleating activity.

In Vivo and In Vitro Isolation of Toxoplasma gondii Resistant Strains from Pregnant Women with Clinical and Serological Indications of Acute Toxoplasmosis

Transfusion-Transmitted Pathogens: Evaluation of Anti-Toxoplasma Gondii Antibodies Seroprevalence Among Volunteer Blood Donors Associated to Transfusional Risks at the Official Hemotherapy Center and the Public Health Systems in Curitiba City, Parana, Southern of Brazil

European Journal of Immunology, Sep 13, 2009

Optimum aerodynamic design for wind turbine blade with a Rankine vortex wake

Renewable Energy, 2013

ABSTRACT This paper presents a model to optimize the distribution of chord and twist angle of hor... more ABSTRACT This paper presents a model to optimize the distribution of chord and twist angle of horizontal axis wind turbine blades, taking into account the influence of the wake, by using a Rankine vortex. This model is applied to both large and small wind turbines, aiming to improve the aerodynamics of the wind rotor, and particularly useful for the case of wind turbines operating at low tip-speed ratios. The proposed optimization is based on maximizing the power coefficient, coupled with the general relationship between the axial induction factor in the rotor plane and in the wake. The results show an increase in the chord and a slightly decrease in the twist angle distributions as compared to other classical optimization methods, resulting in an improved aerodynamic shape of the blade. An evaluation of the efficiency of wind rotors designed with the proposed model is developed and compared other optimization models in the literature, showing an improvement in the power coefficient of the wind turbine. Published by Elsevier Ltd.

III – FQRNT Centre for Host-Parasite Interactions Centre de recherche sur les interactions hôte-parasite McGill University - Montreal - Canada

III – FQRNT Centre for Host-Parasite Interactions Centre de recherche sur les interactions hôte-p... more III – FQRNT Centre for Host-Parasite Interactions Centre de recherche sur les interactions hôte-parasite In 2006-2007, our Provincially-funded Regroupement Stratégique Centre completed its fourth busy year of operation. Please visit the website www.mcgill.ca/chpi for details of our scientific activities, and services offered, throughout the Centre. The Centre for Host-Parasite Interactions brings together researchers and their trainees at the Institute of Parasitology, Department of Microbiology and Immunology, Department of Biology, Department of Chemistry, Department of Epidemiology and Biostatistics, Department of Physiology, Department of Chemical Engineering, Department of Bioresource Engineering, School of Dietetics and Human Nutrition, Brace Research Institute, McGill Centre for Tropical Medicine and the McGill University Health Centre, at McGill University, Université de Montréal, Centre de recherche en Infectiologie, Université Laval, INRS – Institut Armand-Frappier, and Un...

Good Quality Scintigraphic Images in Patients with Low 99mTc-HMPAO Leucocyte Labelling Efficiency

Immunological and Molecular Factors Involved on Hard Tissue Resorption int Periodontal Disease

Immunological and Molecular Factors Involved on Hard Tissue Resorption in the Periodontal Disease... more Immunological and Molecular Factors Involved on Hard Tissue Resorption in the Periodontal Disease anisms and causes involved in the hard tissue resorption in periodontal focal sites. This disease is widely disseminated through our population, and thus require the comprehension of its immunophysiopathological mechanisms, in order to improve and develop preventive measures and treatment. Within periodontal sites is located the dentoalveolar articulation, rich in cells, fibers, microcirculation vases and nerves, and it is in this microenvironment that tissue changes may occur in response to the Innate and Acquired Immune System activation. The activation of specific cells induced by cytokines, growth factors, proteins and hormones seems to be the main remodulation mechanism of hard tissue. Neverthe- less, the cell to cell contact during the activation of the resorp- tion process, in the presence or absence of LPS producing bac- teria, could use receptor activator NF-kB (RANK), and its ...

Origem e Tipos de Fagócitos. Phagocytes: Types & Origin

Os fagócitos são componentes celulares da imunidade inata ou natural responsáveis pela primeira l... more Os fagócitos são componentes celulares da imunidade inata ou natural responsáveis pela primeira linha de defesa do organismo contra infecções, eliminando os microrganismos invasores (patógenos) através de um mecanismo efetor denominado fagocitose. O presente estudo apresenta uma revisão sobre os tipos de células fagocíticas, bem como sobre sua origem e maturação. Palavras-chave: fagócitos, granulócitos polimorfonucleares, neutrófilos, monócitos e macrófagos.

Biotecnologia na Indústria Farmacêutica

A indústria farmacêutica necessita de processos biotecnológicos para obtenção de vários produtos ... more A indústria farmacêutica necessita de processos biotecnológicos para obtenção de vários produtos importantes para a saúde humana e animal. A história da biotecnologia moderna começa inclusive com o desenvolvimento de um medicamento, a penicilina, na primeira metade do século XX. A partir de então, processos biotecnológicos são utilizados na produ ção de vitaminas, hormônios, antibióticos, vacinas e enzimas. Este trabalho apresenta as características gerais desses processos.

Depressão / Evidências Genético-Moleculares De Vulnerabilidade a Distúrbios / Doenças Afetivas

Rogério Saad Vaz1 ,BMD,PhD 1-Biomédico, Pós-grad. em Imunologia pela Universidade Livre de Bruxel... more Rogério Saad Vaz1 ,BMD,PhD 1-Biomédico, Pós-grad. em Imunologia pela Universidade Livre de Bruxelas e Univ. Estadualde Gent - Bélgica, PhD em Engenharia de Bioprocessos e Biotecnologia pela UFPR (Área - Saúde Humana e Animal) - UFPR rogeriovaz@fpp.edu.br DEPRESSÃO: EVIDÊNCIAS GENÉTICO-MOLECULARES DE VULNERABILIDADE A DISTÚRBIOS E DOENÇAS AFETIVAS. Em estudos de base genética humana e clínica relacionados a doenças afetivas,o transtorno afetivo bipolar tem sido o mais freqüentemente estudado, em função da relação de herdabilidade deste transtorno. Localização - Cromossômica A região de maior probabilidade de relação com doença bipolar está localizada no braço longo do cromossomo X (Xq28).Winokur et al. (1969) e Mendlewicz et al. (1974) demonstraram uma cosegregação da doença maníaco-depressiva com a cegueira para cores. MendlewicZ et al. (1980) pareceram confirmar este achado inicial, observando uma ligação entre a deficiência da enzima glicose-6-fosfato desidrogenase (G-6-PD) com di...

HTLV-I/II Detection by Low Cost PCR of Diluted and Pooled Blood Samples

JAIDS Journal of Acquired Immune Deficiency Syndromes

The aim of this study was to evaluate HTLV I/II sensitivity of pooled and serial diluted samples ... more The aim of this study was to evaluate HTLV I/II sensitivity of pooled and serial diluted samples by PCR (polumerase Chain Reaction). This could provide the possibility of epidemilogical studies using this methodology. Samples of 4 HTLV I (+) (ELISA/WB) individuals confirmed by PCR (Roche Molecular Sustems KIT) were analysed. The samples (whole blood/ pelleted leukocytes) were diluted and amplified by PCR, and the products were detected by Enzyme Immune Assay (specific probes). The results were calculated according to OD/CO values. The established cut off was 0.250. All samples were also tested for HTLV II and resulted negative. All samples (100%) were positive to the dilution of 1/50, 3 were positive (37.5%) and 5 were negative (62.5%) for the dilution 1/100. We concluded that the dilution to 1/50 of pooled samples seemend to be efficient. In comparison with serological tests, PCR of pooled samples is by itself a confirming tes. Further studies on PCR diluted samples have to be done...

1.Toxoplasmosis & Pregnacy – Curitiba (Parana -Southern of Brazil). 2. Transfusions Risks and Toxoplasmosis III – FQRNT Centre for Host-Parasite Interactions Centre de recherche sur les interactions hôte-parasite (McGill Univ. Montreal Canada)

The Centre for Host-Parasite Interactions brings together researchers and their trainees at the I... more The Centre for Host-Parasite Interactions brings together researchers and their trainees at the Institute of Parasitology, Department of Microbiology and Immunology, Department of Biology, Department of Chemistry, Department of Epidemiology and Biostatistics, Department of Physiology, Department of Chemical Engineering, Department of Bioresource Engineering, School of Dietetics and Human Nutrition, Brace Research Institute, McGill Centre for Tropical Medicine and the McGill University Health Centre, at McGill University, Université de Montréal, Centre de recherche en Infectiologie, Université Laval, INRS – Institut Armand-Frappier, and Université du Québec à Montréal into what is considered by external reviewers as the strongest centre for parasitological research in the world. It is recognized as an FQRNT Regroupement stratégique. The research of the Centre focuses on Mechanisms of Pathogenesis and Host Defense to Parasite Infections, and the Molecular Basis of Anti-parasite Drug R...

Technical Requirement for Implementation of Sorological Tests for the Detection of Toxoplasma Gondii in Routinely Services of Hemotherapy with Voluntary Blood Donators of the Official Net in Curitiba City, Paraná, Southern of Brazil

ABSTRACT Serological assays to detect anti-Toxoplasma gondii antibodies and specific anamnesis as... more ABSTRACT Serological assays to detect anti-Toxoplasma gondii antibodies and specific anamnesis associated with this highly prevalent infection could be necessary in hemotherapy services in order to regulate safer blood supplies thus avoiding unnecessary transfusion risks. Even though, such policies are not yet implemented. In order to evaluate this requirement, our research group designed a questionnaire filled up by 132 volunteer blood donors from Parana Hemotherapy Center (Hemepar) assessing the possible risk factors to toxoplasmic infection. A total of 20 IgG anti-Toxoplasma serological tests were done to check out for positive-reactivity. 60% of the selected serum samples reacted positively; from these, 50% reported having pets (average of 2 animals per person) and 33% of these pets were "semi-free". It suggested the probability of toxoplasmic infection through these animals. Only 2.2% of the interviewed individuals were aware of the correct concept of the disease and ...