Introduction. Placentation and autochthonous vascularization during the early period of intrauter... more Introduction. Placentation and autochthonous vascularization during the early period of intrauterine development in primary placental insufficiency are important stages in the establishment of embryo-haemochorial circulation. Vascular and angiogenesis dysfunctions in the early period of gestation are essential in the development of changes in placental vascular status with severe repercussions on pregnancy outcome in the first trimester of gestation. The purpose of the work. Evaluation of vascular density by the application of the anti-CD31 antibody in the chorio-villar germinal site in short-term dysregulated pregnancies. Material and methods. 184 cases were divided into 2 groups: stagnant pregnancies (SP (L1 = 144 cases) and early spontaneous abortion (ESA) (L2 = 40 cases). Control material: pregnancies resolved at social indications/desire (A) (n = 18) distributed according to gestational term (3-5 weeks), (6-9 weeks), and (10-12weeks). The estimation of the histomorphological features was performed by applying the classical conventional method with hematoxylin-eosin (H.E.), and the immunohistochemical evaluation of the vascular density in the chorio-villar profile with the help of the endothelial marker anti-CD31. The statistical relationships between fetal conceptus assessment forms, gestational term, and age were analyzed. Results. Distribution of pregnancies by profile: (SP)-78.2% vs (ESA)-21.8%, with vascular disturbances attested in 100% of cases by increasing the density of avascular chorionic villi in the case of stagnant pregnancies in 75% of cases. The application of immunoexpression with anti-CD31 at the level of the stroma of the chorionic villi determined PVI with a maximum mean in the control group (91.51±0.71) vs the ESA and SP groups (82.29±12.96 and 57.47±6.53, respectively). The index of vascularization with lumen (IV/L+) and vascularization index without lumen (IV/L-) were statistically significantly different (p = 0.011) for (SP). Applying the student t-test revealed statistically significant differences for PVI in most groups, except PSD vs SP at the age of 10-12 gestation of weeks (w.g.) At the same time, there are statistically significant differences between the means of vessels with lumen within PSD and SP (t31 = 5.500, p = 0.000005), and the means of vessels without lumen registered statistically significant differences in the PSD and SP groups (t12 = 3.862, p = 0.002). Conclusions. Pregnancy evolution depends on the degree of vascularization, as well as on the quality of the vascular network. Disruption of placental vasculature is one of the basic links in the development of primary placental insufficiency during placentation.
Introduction. Breast cancer is one of the most common cancers in females worldwide. There are evi... more Introduction. Breast cancer is one of the most common cancers in females worldwide. There are evidences that women with diabetes mellitus have a 40% higher risk of mortality. CD34 is a cell surface glycoprotein, which functions as a cellcell adhesion factor. Although its expression is traditionally related to hematopoietic cells, it is actually found on many other types of cells, endothelial too. Nowadays there are evidences that CD34 is a prognostic indicator by emphasizing its low expression in malignant tumors compared to benign ones. The aim of study was to determine the presence and numerical distribution of CD34+ vessels in the normal mammary gland, as well as in NST breast carcinomas, with and without diabetes mellitus type 2. Materials and methods. We processed immunohistochemically 58 invasive breast carcinomas of NST type. In 29 of cases, tumors were associated with diabetes. Results. The present study did not reveal any statistical and morphological differences in CD34 ex...
Ateroscleroza reprezintă o cauză de bază în dezvoltarea bolii ischemice a cordului, ictusului, af... more Ateroscleroza reprezintă o cauză de bază în dezvoltarea bolii ischemice a cordului, ictusului, afecţiunilor arterelor membrelor inferioare şi a altor organe. Iniţierea aterosclerozei este favorizatăde o varietate mare de factori etiologici şi patogenetici, care acţionează atât independent, cât şi în combinaţie unii cu alţii. Nivelul înalt al homocisteinei în plasmă este considerat ca factor de risc al patologiei cerebro- şi cardiovasculare, mai frecvent întâlnite la femei decât la bărbaţi.Ateroscleroza reprezintă o cauză de bază în dezvoltarea bolii ischemice a cordului, ictusului, afecţiunilor arterelor membrelor inferioare şi a altor organe. Iniţierea aterosclerozei este favorizatăde o varietate mare de factori etiologici şi patogenetici, care acţionează atât independent, cât şi în combinaţie unii cu alţii. Nivelul înalt al homocisteinei în plasmă este considerat ca factor de risc al patologiei cerebro- şi cardiovasculare, mai frecvent întâlnite la femei decât la bărbaţi.Ateroscle...
Tumorile hipofizare sunt procese patologice la nivelul hipofizei, sunt hormonal active, cu locali... more Tumorile hipofizare sunt procese patologice la nivelul hipofizei, sunt hormonal active, cu localizare la baza creierului. Hipofiza, deși au dimensiuni reduse, are un rol foarte important în reglarea metabolismului și participă la maturarea organismului în general. Tumorile hipofizare reprezintă 10-25% din totalitatea tumorilor cu localizare intracerebrală, au o rata de creștere foarte lentă și nu au capacitatea de a metastaza. În pofida faptului că sunt benigne din punct de vedere histologic au un efect biologic malign și din acest motiv necesită să fi e calitativ diagnosticate. La ora actuală realizarea profilului imunohistochimic permite diagnosticarea funcției secretorii a tumorii și prescrierea tratamentului personalizat
Background: Tumor growth and development is determined by the mutual interaction between the canc... more Background: Tumor growth and development is determined by the mutual interaction between the cancer cells themselves and the microenvironment. It contains various elements, including immune cells. Of all, mast cells have one of the most controversial roles. The aim of the present study was to evaluate the expression of mast cell tryptase in the luminal and non-luminal subtypes of breast cancer and establish a possible link between infiltration with MCs and expression of hormone receptors. Material and methods: The experimental study included 80 cases of breast carcinomas that were analyzed immunohistochemically in order to establish the molecular profile and the expression of tryptase, a specific marker of mast cells. The data were processed using the SPSS program. Pearson’s coefficient (r) and the other values were considered statistically significant in case of p≤0.05. Results: Both intratumoral mast cells (MCit) and peritumoral mast cells (MCpt) correlated with the expression of ...
Introduction. Placentation and autochthonous vascularization during the early period of intrauter... more Introduction. Placentation and autochthonous vascularization during the early period of intrauterine development in primary placental insufficiency are important stages in the establishment of embryo-haemochorial circulation. Vascular and angiogenesis dysfunctions in the early period of gestation are essential in the development of changes in placental vascular status with severe repercussions on pregnancy outcome in the first trimester of gestation. The purpose of the work. Evaluation of vascular density by the application of the anti-CD31 antibody in the chorio-villar germinal site in short-term dysregulated pregnancies. Material and methods. 184 cases were divided into 2 groups: stagnant pregnancies (SP (L1 = 144 cases) and early spontaneous abortion (ESA) (L2 = 40 cases). Control material: pregnancies resolved at social indications/desire (A) (n = 18) distributed according to gestational term (3-5 weeks), (6-9 weeks), and (10-12weeks). The estimation of the histomorphological features was performed by applying the classical conventional method with hematoxylin-eosin (H.E.), and the immunohistochemical evaluation of the vascular density in the chorio-villar profile with the help of the endothelial marker anti-CD31. The statistical relationships between fetal conceptus assessment forms, gestational term, and age were analyzed. Results. Distribution of pregnancies by profile: (SP)-78.2% vs (ESA)-21.8%, with vascular disturbances attested in 100% of cases by increasing the density of avascular chorionic villi in the case of stagnant pregnancies in 75% of cases. The application of immunoexpression with anti-CD31 at the level of the stroma of the chorionic villi determined PVI with a maximum mean in the control group (91.51±0.71) vs the ESA and SP groups (82.29±12.96 and 57.47±6.53, respectively). The index of vascularization with lumen (IV/L+) and vascularization index without lumen (IV/L-) were statistically significantly different (p = 0.011) for (SP). Applying the student t-test revealed statistically significant differences for PVI in most groups, except PSD vs SP at the age of 10-12 gestation of weeks (w.g.) At the same time, there are statistically significant differences between the means of vessels with lumen within PSD and SP (t31 = 5.500, p = 0.000005), and the means of vessels without lumen registered statistically significant differences in the PSD and SP groups (t12 = 3.862, p = 0.002). Conclusions. Pregnancy evolution depends on the degree of vascularization, as well as on the quality of the vascular network. Disruption of placental vasculature is one of the basic links in the development of primary placental insufficiency during placentation.
Introduction. Breast cancer is one of the most common cancers in females worldwide. There are evi... more Introduction. Breast cancer is one of the most common cancers in females worldwide. There are evidences that women with diabetes mellitus have a 40% higher risk of mortality. CD34 is a cell surface glycoprotein, which functions as a cellcell adhesion factor. Although its expression is traditionally related to hematopoietic cells, it is actually found on many other types of cells, endothelial too. Nowadays there are evidences that CD34 is a prognostic indicator by emphasizing its low expression in malignant tumors compared to benign ones. The aim of study was to determine the presence and numerical distribution of CD34+ vessels in the normal mammary gland, as well as in NST breast carcinomas, with and without diabetes mellitus type 2. Materials and methods. We processed immunohistochemically 58 invasive breast carcinomas of NST type. In 29 of cases, tumors were associated with diabetes. Results. The present study did not reveal any statistical and morphological differences in CD34 ex...
Ateroscleroza reprezintă o cauză de bază în dezvoltarea bolii ischemice a cordului, ictusului, af... more Ateroscleroza reprezintă o cauză de bază în dezvoltarea bolii ischemice a cordului, ictusului, afecţiunilor arterelor membrelor inferioare şi a altor organe. Iniţierea aterosclerozei este favorizatăde o varietate mare de factori etiologici şi patogenetici, care acţionează atât independent, cât şi în combinaţie unii cu alţii. Nivelul înalt al homocisteinei în plasmă este considerat ca factor de risc al patologiei cerebro- şi cardiovasculare, mai frecvent întâlnite la femei decât la bărbaţi.Ateroscleroza reprezintă o cauză de bază în dezvoltarea bolii ischemice a cordului, ictusului, afecţiunilor arterelor membrelor inferioare şi a altor organe. Iniţierea aterosclerozei este favorizatăde o varietate mare de factori etiologici şi patogenetici, care acţionează atât independent, cât şi în combinaţie unii cu alţii. Nivelul înalt al homocisteinei în plasmă este considerat ca factor de risc al patologiei cerebro- şi cardiovasculare, mai frecvent întâlnite la femei decât la bărbaţi.Ateroscle...
Tumorile hipofizare sunt procese patologice la nivelul hipofizei, sunt hormonal active, cu locali... more Tumorile hipofizare sunt procese patologice la nivelul hipofizei, sunt hormonal active, cu localizare la baza creierului. Hipofiza, deși au dimensiuni reduse, are un rol foarte important în reglarea metabolismului și participă la maturarea organismului în general. Tumorile hipofizare reprezintă 10-25% din totalitatea tumorilor cu localizare intracerebrală, au o rata de creștere foarte lentă și nu au capacitatea de a metastaza. În pofida faptului că sunt benigne din punct de vedere histologic au un efect biologic malign și din acest motiv necesită să fi e calitativ diagnosticate. La ora actuală realizarea profilului imunohistochimic permite diagnosticarea funcției secretorii a tumorii și prescrierea tratamentului personalizat
Background: Tumor growth and development is determined by the mutual interaction between the canc... more Background: Tumor growth and development is determined by the mutual interaction between the cancer cells themselves and the microenvironment. It contains various elements, including immune cells. Of all, mast cells have one of the most controversial roles. The aim of the present study was to evaluate the expression of mast cell tryptase in the luminal and non-luminal subtypes of breast cancer and establish a possible link between infiltration with MCs and expression of hormone receptors. Material and methods: The experimental study included 80 cases of breast carcinomas that were analyzed immunohistochemically in order to establish the molecular profile and the expression of tryptase, a specific marker of mast cells. The data were processed using the SPSS program. Pearson’s coefficient (r) and the other values were considered statistically significant in case of p≤0.05. Results: Both intratumoral mast cells (MCit) and peritumoral mast cells (MCpt) correlated with the expression of ...
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