Background & Aim: Nowadays, according to the advances in medical science, blood products are cons... more Background & Aim: Nowadays, according to the advances in medical science, blood products are considered as one of the important biological tools. In this regard, the use of blood-derived biomaterials in reconstructive medicine to repair a variety of tissues has expanded. One of these products is based on platelet separation, which is obtained by different processing techniques and is used alone or in combination with a variety of synthetic or natural structures. These platelet products contain a large number of growth factors that play key roles in the process of tissue repair and regeneration. The purpose of this study was to investigate the importance of these products in the repair of different body tissues. Materials & Methods: This study is a review one that was conducted by electronic search in PubMed and EMBASE databases from 2000 to 2021 with keywords such as platelet⸲ growth factors⸲ stem cells and regenerative medicine. Results: Findings from studies show that blood-derived biomaterials can be extracted by different techniques, and these products have different types of growth factors such as VEGF, PDGF, EGF, and TGF-β, which can contribute to faster tissue regeneration alone or in combination with other substances. Conclusion: Studies show that blood-derived platelet products can facilitate and accelerate the healing process of various tissues. They can also help advance therapeutic goals.
Objective: Tennis elbow is damage of tendon fibers that happens in any age group. It appears with... more Objective: Tennis elbow is damage of tendon fibers that happens in any age group. It appears with severe pain and inflammation and functional limitations. Now days, none operative methods have been considered. PRP (platelet rich plasma) injections are helpful in treating tennis elbow. Methods: In this method, whole blood obtained and concentrated the platelets, and injected into elbow. After that, patient should wear splint for month. Results: it leads one month to see the evidence of recovery in pain and range of motion in patient. Conclusion: We found that PRP injection can be considered as a safe and effective tool for intractable lateral epicondylitis of the elbow and eliminated the need for surgical intervention.
Background: The use of umbilical cord blood as a source of hematopoietic stem cells for the treat... more Background: The use of umbilical cord blood as a source of hematopoietic stem cells for the treatment of over 80 disorders has been established. Given the general availability, the ease of procurement, and progenitor content, cord blood is an attractive replacement for bone marrow. Expansion and proliferation of undifferentiated cord blood- hematopoietic stem cells in vitro is limited and insufficient. Most of the in vitro cell culture systems currently used for expansion and proliferation of hematopoietic stem cells are two dimensional (2D) culture systems. It is important that three dimensional (3D) scaffolds, providing appropriate environment for proliferation and expansion of hematopoietic stem cells. The aim of present study is to use the fibrin 3D biomaterial as a suitable environment for in vitro expansion and proliferation of cord blood- hematopoietic stem cells. Material and methods: In this study, hematopoietic stem cells were isolated from freshly umbilical cord blood and were recovered by hydroxyl ethyl starch (HES) and phosphate buffer saline (PBS). Also, fibrin glue biomaterial was prepared. Isolated cells were cultured separately on the 3D fibrin glue scaffold and 2D culture environment as control and 2 weeks after expansion, cell proliferation ability was evaluated using MTT assay. Results: Our findings demonstrated that the cell proliferation of cord blood- hematopoietic stem cells is enhanced in a 3D fibrin scaffold compared to control. This difference was statically significant (p<0.05). Conclusion: According to the results of the study, it can be said that natural scaffolds such as fibrin can be used as an appropriate environment for hematopoietic stem cells proliferation.
Journal of Rafsanjan University of Medical Sciences, 2016
Background and Objectives: Nowadays, cell therapy is one of the most important and promising stra... more Background and Objectives: Nowadays, cell therapy is one of the most important and promising strategies in the treatment of diseases. Unique capabilities of stem cells caused them to be used in both research and treatment as a valuable resource in basic science and medical researches. The use of stem cells has been limited due to the related ethical problems. One of the major concerns of scientists to develop and use more of this science is finding alternative methods for producing these cells. Creating induced pluripotent stem cells (IPSCs) is one of the most important methods in achieving this goal. In this regard, various methods are developing that have the ability to change the gene expression and protein profiles, which results in a change in cells morphology and function in the direction of returning to a undifferentiated status and creating stem cells. These methods include nuclear transfer, use of cell extracts and synthetic molecules, mandatory expression of specific genes...
adapt in the avascular niche of the IVD. For this therapeutic strategy to succeed, the indication... more adapt in the avascular niche of the IVD. For this therapeutic strategy to succeed, the indications for its use and the patients who would benefit need to be better defined. To surmount these obstacles, the solution will be identified only by focused research, both in the laboratory and in the clinic. In present paper, the potential utilization of different adult stem cells for intervertebral disc regeneration has been reported. Bone marrow mesenchymal stem cells, adipose tissue derived stem cells, synovial stem cells and committed IVD cells have been studied for this purpose either in vitro or in vivo.
Intervertebral disc degeneration (IDD) is a prevalent disease that usually starts from the third ... more Intervertebral disc degeneration (IDD) is a prevalent disease that usually starts from the third decade of life and it is considered as one of the reasons contributing to social and economic problems. The results of several studies have shown that platelet-rich plasma (PRP) has the ability to stimulate cell growth and proliferation of extracellular matrix. However, studies for IDD have so far mostly been restricted. So, further studies are required to clarify the role of PRP on the prevention and treatment of the IDD disease. The purpose of this review article was to summarize and analyze the current preclinical studies and clinical evidence on using platelet-rich plasma in the IDD treatment. Literature search was performed through various combinations of the following keywords: Intervertebral Disc Degeneration, Platelet Rich Plasma, PRP, Intervertebral disc regeneration. Papers included in our review cover the period between 2006 and 2016. At the end of the review process, articles...
Tehran University Medical Journal TUMS Publications, 2018
cess of chondrogenic differentiation of adipose-derived stem cells. Furthermore, in several studi... more cess of chondrogenic differentiation of adipose-derived stem cells. Furthermore, in several studies, the effect of several miRNAs has been confirmed on the regulation of the cartilage differentiation of the adipose-derived stem cells and has also been associated with effective results. In this article, we will present an overview of the advance in adiposederived stem cells application in cartilage regeneration.
Background: One of the most conventional causes of male infert lity is increasing age, which has ... more Background: One of the most conventional causes of male infert lity is increasing age, which has significant effects on sperm physiology. In advanced ages, dege nerative alterations in germinal epithelium, decrea sed number of Leydig cells, and their functions affect spermatogenesis through a decrease in testosterone l vel. So it is important to know whether advanced paternal age is associated with decreased semen quality. Methods: Semen samples of 144 men were collected and semen parameters (volume, concentration, and normal morphology) were evaluated after semen liquefaction at room temperature. Statistical analyzes of data w s done using SPSS software. Results: we showed that increased total sperm count was cor related with increasing age (P ≤0. 05). Analyzing the percentage of normal morphology showed a signif icant difference between group I and III (p ≤0. 05) while there was no significant difference in comparison o f other groups. In terms of the semen volume, no si gnificant...
Introduction: Natural biomaterials and growth factors are key factors in tissue engineering. The ... more Introduction: Natural biomaterials and growth factors are key factors in tissue engineering. The objective of the present study was to evaluate transforming growth factor beta 1 (TGF-β1) and curcumin on proliferation and differentiation of nasal-derived chondrocyte seeded on the fibrin glue scaffold. Methods: Chondrocytes were isolated from nasal samples. Nasal-derived chondrocytes were seeded on fibrin glue at chondrogenic induction medium for 2 weeks. In this study, the effects of various concentrations of curcumin and TGF-β1 on the survival and proliferation of chondrocytes seeded on fibrin biomaterial were assessed by MTT assays. Also, chondrocyte-specific gene expression was assessed by real-time polymerase chain reaction (PCR). Results: There were significant differences among the group treated with curcumin 10 µg compared to other groups with regard to cell viability. Also, gene expression of collagen type II, aggrecan, and SOX9 in the chondrocytes seeded on fibrin biomateria...
Background and Objectives: There are very few analysis tools to examine seminal fluid specimens, ... more Background and Objectives: There are very few analysis tools to examine seminal fluid specimens, so bacterial infections on male infertility has always been the subject of discussion. These infectious processes lead to deterioration of spermatogenesis, impairment of sperm function, and/or obstruction of the seminal tract. In this study, we aimed at determining the role of bacterial infection on semen parameters including motility, count and normal morphology in infertile male patients. Materials and Methods: In this cohort study, 150 infertile males having abnormal semen parameters (study group) and 150 healthy fertile males (control group) were included. A total of 300 semen samples were collected after 3 to 5 days of sexual abstinence. Volume, pH, concentration, normal morphology, and motility were evaluated. Samples were seeded using a calibrated loop on agar and EMB plates, which were incubated overnight. The microorganisms were identified by Gram staining technique, catalase an...
Problems: Contamination of cell lines and biological products is one of the major problems of cel... more Problems: Contamination of cell lines and biological products is one of the major problems of cell culture techniques. Rapid detection of mycoplasma contamination in cell culture is an important part of controlled laboratory. In order to existence of infected cells, leading to unreliable results, the need for a reliable method for laboratories is unavoidable. Polymerase chain reaction (PCR), a technique of rapid, sensitive and specific detection of bacteria is considered as a precise method to detect contamination. The aim of this study was to evaluate the efficacy of PCR in the detection of contaminants in cell cultures and other biological products.
Background & Aim: Nowadays, according to the advances in medical science, blood products are cons... more Background & Aim: Nowadays, according to the advances in medical science, blood products are considered as one of the important biological tools. In this regard, the use of blood-derived biomaterials in reconstructive medicine to repair a variety of tissues has expanded. One of these products is based on platelet separation, which is obtained by different processing techniques and is used alone or in combination with a variety of synthetic or natural structures. These platelet products contain a large number of growth factors that play key roles in the process of tissue repair and regeneration. The purpose of this study was to investigate the importance of these products in the repair of different body tissues. Materials & Methods: This study is a review one that was conducted by electronic search in PubMed and EMBASE databases from 2000 to 2021 with keywords such as platelet⸲ growth factors⸲ stem cells and regenerative medicine. Results: Findings from studies show that blood-derived biomaterials can be extracted by different techniques, and these products have different types of growth factors such as VEGF, PDGF, EGF, and TGF-β, which can contribute to faster tissue regeneration alone or in combination with other substances. Conclusion: Studies show that blood-derived platelet products can facilitate and accelerate the healing process of various tissues. They can also help advance therapeutic goals.
Objective: Tennis elbow is damage of tendon fibers that happens in any age group. It appears with... more Objective: Tennis elbow is damage of tendon fibers that happens in any age group. It appears with severe pain and inflammation and functional limitations. Now days, none operative methods have been considered. PRP (platelet rich plasma) injections are helpful in treating tennis elbow. Methods: In this method, whole blood obtained and concentrated the platelets, and injected into elbow. After that, patient should wear splint for month. Results: it leads one month to see the evidence of recovery in pain and range of motion in patient. Conclusion: We found that PRP injection can be considered as a safe and effective tool for intractable lateral epicondylitis of the elbow and eliminated the need for surgical intervention.
Background: The use of umbilical cord blood as a source of hematopoietic stem cells for the treat... more Background: The use of umbilical cord blood as a source of hematopoietic stem cells for the treatment of over 80 disorders has been established. Given the general availability, the ease of procurement, and progenitor content, cord blood is an attractive replacement for bone marrow. Expansion and proliferation of undifferentiated cord blood- hematopoietic stem cells in vitro is limited and insufficient. Most of the in vitro cell culture systems currently used for expansion and proliferation of hematopoietic stem cells are two dimensional (2D) culture systems. It is important that three dimensional (3D) scaffolds, providing appropriate environment for proliferation and expansion of hematopoietic stem cells. The aim of present study is to use the fibrin 3D biomaterial as a suitable environment for in vitro expansion and proliferation of cord blood- hematopoietic stem cells. Material and methods: In this study, hematopoietic stem cells were isolated from freshly umbilical cord blood and were recovered by hydroxyl ethyl starch (HES) and phosphate buffer saline (PBS). Also, fibrin glue biomaterial was prepared. Isolated cells were cultured separately on the 3D fibrin glue scaffold and 2D culture environment as control and 2 weeks after expansion, cell proliferation ability was evaluated using MTT assay. Results: Our findings demonstrated that the cell proliferation of cord blood- hematopoietic stem cells is enhanced in a 3D fibrin scaffold compared to control. This difference was statically significant (p<0.05). Conclusion: According to the results of the study, it can be said that natural scaffolds such as fibrin can be used as an appropriate environment for hematopoietic stem cells proliferation.
Journal of Rafsanjan University of Medical Sciences, 2016
Background and Objectives: Nowadays, cell therapy is one of the most important and promising stra... more Background and Objectives: Nowadays, cell therapy is one of the most important and promising strategies in the treatment of diseases. Unique capabilities of stem cells caused them to be used in both research and treatment as a valuable resource in basic science and medical researches. The use of stem cells has been limited due to the related ethical problems. One of the major concerns of scientists to develop and use more of this science is finding alternative methods for producing these cells. Creating induced pluripotent stem cells (IPSCs) is one of the most important methods in achieving this goal. In this regard, various methods are developing that have the ability to change the gene expression and protein profiles, which results in a change in cells morphology and function in the direction of returning to a undifferentiated status and creating stem cells. These methods include nuclear transfer, use of cell extracts and synthetic molecules, mandatory expression of specific genes...
adapt in the avascular niche of the IVD. For this therapeutic strategy to succeed, the indication... more adapt in the avascular niche of the IVD. For this therapeutic strategy to succeed, the indications for its use and the patients who would benefit need to be better defined. To surmount these obstacles, the solution will be identified only by focused research, both in the laboratory and in the clinic. In present paper, the potential utilization of different adult stem cells for intervertebral disc regeneration has been reported. Bone marrow mesenchymal stem cells, adipose tissue derived stem cells, synovial stem cells and committed IVD cells have been studied for this purpose either in vitro or in vivo.
Intervertebral disc degeneration (IDD) is a prevalent disease that usually starts from the third ... more Intervertebral disc degeneration (IDD) is a prevalent disease that usually starts from the third decade of life and it is considered as one of the reasons contributing to social and economic problems. The results of several studies have shown that platelet-rich plasma (PRP) has the ability to stimulate cell growth and proliferation of extracellular matrix. However, studies for IDD have so far mostly been restricted. So, further studies are required to clarify the role of PRP on the prevention and treatment of the IDD disease. The purpose of this review article was to summarize and analyze the current preclinical studies and clinical evidence on using platelet-rich plasma in the IDD treatment. Literature search was performed through various combinations of the following keywords: Intervertebral Disc Degeneration, Platelet Rich Plasma, PRP, Intervertebral disc regeneration. Papers included in our review cover the period between 2006 and 2016. At the end of the review process, articles...
Tehran University Medical Journal TUMS Publications, 2018
cess of chondrogenic differentiation of adipose-derived stem cells. Furthermore, in several studi... more cess of chondrogenic differentiation of adipose-derived stem cells. Furthermore, in several studies, the effect of several miRNAs has been confirmed on the regulation of the cartilage differentiation of the adipose-derived stem cells and has also been associated with effective results. In this article, we will present an overview of the advance in adiposederived stem cells application in cartilage regeneration.
Background: One of the most conventional causes of male infert lity is increasing age, which has ... more Background: One of the most conventional causes of male infert lity is increasing age, which has significant effects on sperm physiology. In advanced ages, dege nerative alterations in germinal epithelium, decrea sed number of Leydig cells, and their functions affect spermatogenesis through a decrease in testosterone l vel. So it is important to know whether advanced paternal age is associated with decreased semen quality. Methods: Semen samples of 144 men were collected and semen parameters (volume, concentration, and normal morphology) were evaluated after semen liquefaction at room temperature. Statistical analyzes of data w s done using SPSS software. Results: we showed that increased total sperm count was cor related with increasing age (P ≤0. 05). Analyzing the percentage of normal morphology showed a signif icant difference between group I and III (p ≤0. 05) while there was no significant difference in comparison o f other groups. In terms of the semen volume, no si gnificant...
Introduction: Natural biomaterials and growth factors are key factors in tissue engineering. The ... more Introduction: Natural biomaterials and growth factors are key factors in tissue engineering. The objective of the present study was to evaluate transforming growth factor beta 1 (TGF-β1) and curcumin on proliferation and differentiation of nasal-derived chondrocyte seeded on the fibrin glue scaffold. Methods: Chondrocytes were isolated from nasal samples. Nasal-derived chondrocytes were seeded on fibrin glue at chondrogenic induction medium for 2 weeks. In this study, the effects of various concentrations of curcumin and TGF-β1 on the survival and proliferation of chondrocytes seeded on fibrin biomaterial were assessed by MTT assays. Also, chondrocyte-specific gene expression was assessed by real-time polymerase chain reaction (PCR). Results: There were significant differences among the group treated with curcumin 10 µg compared to other groups with regard to cell viability. Also, gene expression of collagen type II, aggrecan, and SOX9 in the chondrocytes seeded on fibrin biomateria...
Background and Objectives: There are very few analysis tools to examine seminal fluid specimens, ... more Background and Objectives: There are very few analysis tools to examine seminal fluid specimens, so bacterial infections on male infertility has always been the subject of discussion. These infectious processes lead to deterioration of spermatogenesis, impairment of sperm function, and/or obstruction of the seminal tract. In this study, we aimed at determining the role of bacterial infection on semen parameters including motility, count and normal morphology in infertile male patients. Materials and Methods: In this cohort study, 150 infertile males having abnormal semen parameters (study group) and 150 healthy fertile males (control group) were included. A total of 300 semen samples were collected after 3 to 5 days of sexual abstinence. Volume, pH, concentration, normal morphology, and motility were evaluated. Samples were seeded using a calibrated loop on agar and EMB plates, which were incubated overnight. The microorganisms were identified by Gram staining technique, catalase an...
Problems: Contamination of cell lines and biological products is one of the major problems of cel... more Problems: Contamination of cell lines and biological products is one of the major problems of cell culture techniques. Rapid detection of mycoplasma contamination in cell culture is an important part of controlled laboratory. In order to existence of infected cells, leading to unreliable results, the need for a reliable method for laboratories is unavoidable. Polymerase chain reaction (PCR), a technique of rapid, sensitive and specific detection of bacteria is considered as a precise method to detect contamination. The aim of this study was to evaluate the efficacy of PCR in the detection of contaminants in cell cultures and other biological products.
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