Papers by mirawati soediro
Pharmacia
Dengue fever is widely prevalent in Indonesia and many other tropical countries. There has been n... more Dengue fever is widely prevalent in Indonesia and many other tropical countries. There has been no antiviral protection against DENV (dengue virus) due to the scarcity of animal models that replicate the symptoms of its infection in humans. Subsequently, Balb/c mice were used to optimize the animal models that were infected with DENV, and measurements of DENV titer, platelet count and leukocytes counts were taken after six days of CA (Cassia alata leaves extract) treatment to examine the inhibitory activity of viral replication on dengue-infected mice. The results showed that CA significantly reduced DENV titer and increased platelet count compared to the control group (P < 0.05) but had no significant effect on leukocyte counts (P > 0.05). Therefore, it could be implied that Cassia alata could be further investigated for medication against DENV, prospectively.
Asian Pacific Journal of Cancer Prevention, 2014
The multidrug resistance phenotype is one of the major problems in development of cancer cell res... more The multidrug resistance phenotype is one of the major problems in development of cancer cell resistance to chemotherapy. Some natural compounds from medicinal plants have demonstrated promising capacity in enhancing anticancer effects in drug resistant cancer cells. We aimed to investigate whether mangiferin might have an ability to re-sensitize MCF-7 breast cancer cells previously treated with short-term doxorubicin in vitro, through the modulation of efflux transporters, P-glycoprotein (P-gp), MRP1 and BCRP. We exposed MCF-7 breast cancer cells pretreated with doxorubicin for 10 days to mangiferin (10, 25 or 50 µM) for 96 hours. Afterwards, we evaluated influence on cell viability and level of mRNA expression of P-gp, MRP1 and BCRP. Doxorubicin given in combination with mangiferin at low concentrations (10 and 25 µM) failed to give significant reduction in cell viability, while at the highest concentrations, the combination significantly reduced cell viability. The mRNA expression analysis of P-gp, MRP1 and BCRP showed that mangiferin had inhibitory effects on P-gp but no effects on MRP1 and BCRP. In conclusion, we suggest that mangiferin at high concentrations can be used as chemosensitizer for doxorubicin therapy. This effect might be attributed by inhibitory effects of mangiferin on P-glycoprotein expression.
Indonesian Journal of Biotechnology, 2016
Dengue infection remains a public health problem in Indonesia. In the framework of the independen... more Dengue infection remains a public health problem in Indonesia. In the framework of the independence of the national product, pursued research and development of raw materials that can be used for diagnostic kit and vaccine candidates. The objective of this study was to express and purify recombinant proteins non-structural NS1 dengue virus serotype 1 (DENV-1) of Indonesia strain (915 846) in Pichia pastoris expression system with “halal” system that can be used for the development of diagnostic kits and vaccines. The dengue virus serotype 1 strain Indonesia (915846) was used to construct the NS1 DENV-1 gene by PCR. Then NS1 DENV-1 gene cloned into the vector pPICZαB and transformed into Pichia pastoris yeast cells, then verified with sequencing. Recombinant protein NS1 DENV-1 was secreted expression in P. pastoris. Characterization of antigenicity was did by ELISA, Western Blot and purified by the method of metal-chelating affinity chromatographic (MCAC) column. The recombinant prot...
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Papers by mirawati soediro