Indonesian Journal of Chemical Science and Technology (IJCST), 2020
Jatropha curcas L is widely planted in people's homes. Jatropha has many bioactive compounds,... more Jatropha curcas L is widely planted in people's homes. Jatropha has many bioactive compounds, including flavonoids, saponins, tannins, and alkaloids. In this research, phytochemical tests and antimicrobial activities of sap and water extraction of Jatropha curcas L leaves on Candida albicans, Aspergillus, and Pseudomonas aeruginosa were carried out. The results of the phytochemical test of sap and water extraction of leaves showed positive results in the test of flavonoids, saponins, alkaloids and tannins. While steroid testing showed negative results. The antimicrobial capabilities of sap and water extraction of leaves to Aspergillus were 10.5 mm and 11 mm and to Pseudomonas Aeruginosa were 15 mm and 11 mm. Whereas the antimicrobial ability of sap and water extraction of leaf to Candida albicans was negative. In this case, it means that Pseudomonas aeruginosa is including microbes that are sensitive to antimicrobial origin of plants.
Helminthostacshyszeylanica is a plant of the family ophioglossaceae known as the tunjuk langit pl... more Helminthostacshyszeylanica is a plant of the family ophioglossaceae known as the tunjuk langit plant. The roots of this plant is used as a 100 days cough remedy, dysentery, nasal disease and lung disease. This plant contains saponins, andflavonoids. This study aims to isolate and characterizethe flavonoid compounds from the root of tunjuk langit. Isolation methods used polar fractionation method. Some 7.5 kg oftunjuk langit wasmacerated with methanol17 L for 3x24 hours later ultrasonicatide for 1 hour. Extract thick about 100 gr then partifioned with methanol mixture and n-hexan 2:1. Methanol fraction of partition result thickened then in chromatography liquid vacuum (VLC) in 12 fraction (n-hexane – etilacetate – methanol). Flavonoid compound obtained after VLC result on fraction 5 and 6 is combined then in flash chromatography. Flavonoid compound such yellow crictal on vial 19 is coded as HZ19. The purity of this compound was analized by TLC and HPLC as well as determination of...
Background: The roots of tunjuk langit (Helminthostacshys zeylanica) have been used traditionally... more Background: The roots of tunjuk langit (Helminthostacshys zeylanica) have been used traditionally in some villages in Indonesia, particularly in Riau Province. Objective: In this study we reported two flavonoids and their antidiabetic activity. Material and methods: Isolation of the metabolites was based on polarity fractionation method. Purification processes were conducted by vacuum liquid chromatography (VLC). Chemical structures were elucidated based on spectroscopy characteristics, including FTIR and 1D/2D NMR. Results: The isolated compounds were identified as ugonin J and K. The antidiabetic activity was measured by α-glucosidase inhibitor assay. The antidiabetic activity of ugonin J was found at IC 50 273,13±0,402 ppm and Ugonin K was found at IC 50 138,21±0,263 ppm (moderately active). Conclusion: Therefore this plant can be used traditionally as antidiabetic medicine.
Indonesian Journal of Chemical Science and Technology (IJCST), 2020
Jatropha curcas L is widely planted in people's homes. Jatropha has many bioactive compounds,... more Jatropha curcas L is widely planted in people's homes. Jatropha has many bioactive compounds, including flavonoids, saponins, tannins, and alkaloids. In this research, phytochemical tests and antimicrobial activities of sap and water extraction of Jatropha curcas L leaves on Candida albicans, Aspergillus, and Pseudomonas aeruginosa were carried out. The results of the phytochemical test of sap and water extraction of leaves showed positive results in the test of flavonoids, saponins, alkaloids and tannins. While steroid testing showed negative results. The antimicrobial capabilities of sap and water extraction of leaves to Aspergillus were 10.5 mm and 11 mm and to Pseudomonas Aeruginosa were 15 mm and 11 mm. Whereas the antimicrobial ability of sap and water extraction of leaf to Candida albicans was negative. In this case, it means that Pseudomonas aeruginosa is including microbes that are sensitive to antimicrobial origin of plants.
Helminthostacshyszeylanica is a plant of the family ophioglossaceae known as the tunjuk langit pl... more Helminthostacshyszeylanica is a plant of the family ophioglossaceae known as the tunjuk langit plant. The roots of this plant is used as a 100 days cough remedy, dysentery, nasal disease and lung disease. This plant contains saponins, andflavonoids. This study aims to isolate and characterizethe flavonoid compounds from the root of tunjuk langit. Isolation methods used polar fractionation method. Some 7.5 kg oftunjuk langit wasmacerated with methanol17 L for 3x24 hours later ultrasonicatide for 1 hour. Extract thick about 100 gr then partifioned with methanol mixture and n-hexan 2:1. Methanol fraction of partition result thickened then in chromatography liquid vacuum (VLC) in 12 fraction (n-hexane – etilacetate – methanol). Flavonoid compound obtained after VLC result on fraction 5 and 6 is combined then in flash chromatography. Flavonoid compound such yellow crictal on vial 19 is coded as HZ19. The purity of this compound was analized by TLC and HPLC as well as determination of...
Background: The roots of tunjuk langit (Helminthostacshys zeylanica) have been used traditionally... more Background: The roots of tunjuk langit (Helminthostacshys zeylanica) have been used traditionally in some villages in Indonesia, particularly in Riau Province. Objective: In this study we reported two flavonoids and their antidiabetic activity. Material and methods: Isolation of the metabolites was based on polarity fractionation method. Purification processes were conducted by vacuum liquid chromatography (VLC). Chemical structures were elucidated based on spectroscopy characteristics, including FTIR and 1D/2D NMR. Results: The isolated compounds were identified as ugonin J and K. The antidiabetic activity was measured by α-glucosidase inhibitor assay. The antidiabetic activity of ugonin J was found at IC 50 273,13±0,402 ppm and Ugonin K was found at IC 50 138,21±0,263 ppm (moderately active). Conclusion: Therefore this plant can be used traditionally as antidiabetic medicine.
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