... Azat ALINAK 1 , Tamer DEMİR 1 , Burak TURGUT 1 , Nusret AKPOLAT 2 , Orhan AYDEMİR 1 , Nesrin ... more ... Azat ALINAK 1 , Tamer DEMİR 1 , Burak TURGUT 1 , Nusret AKPOLAT 2 , Orhan AYDEMİR 1 , Nesrin DEMİR 3. ... Dokularda iskemi kritik bir zaman dilimini aşarsa; ardışık birtakım kimyasal olaylar oluşmakta ve bu olaylar hücre disfonksiyonu, interstisyel ödem, hücresel hasar ve ...
1st, 6th and 24th hour by visual analogue scale (VSA). Supplementary analgesic requirement and po... more 1st, 6th and 24th hour by visual analogue scale (VSA). Supplementary analgesic requirement and possible This present study investigated the effects of intracomplications were also followed. The intensity of articular morphine administration in 1 mg and 5 mg pain and analgesic requirement were reduced more doses on post-operative pain relief and analgesic rein the morphine 5 mg group than in the control group. quirements for patients undergoing arthroscopic pro-It is concluded, that the administration of intraarticular cedures. At the end of the operation patients were morphine 5 mg provides long-lasting and effective randomly allocated in a double-blinded fashion into analgesia after knee arthroscopy. three groups. The control group (Group 1) received normal saline 20 mL intraarticularly. The patients in
Alport syndrome is a type IV collagen synthesis disorder characterized by hereditary progressive ... more Alport syndrome is a type IV collagen synthesis disorder characterized by hereditary progressive glomerular disease resulting from glomerular basement membrane injury, often accompanied by hearing loss and ocular defects. The most common form is X-linked Alport syndrome, which accounts for 80% of all cases. Although women mainly present with mild urinary symptoms, end-stage renal disease onsets at an early age in men. An 11-year-old girl was admitted to our hospital with mild bifissure oedema for the last 3 months. This study discusses the clinical, morphological and transmission electron microscopic findings of a rare case of Alport syndrome in the context of the wider literature.
Bipolar koter kullan›larak yap›lan unilateral proksimal tubal oklüzyon iflleminin birinci ve alt›... more Bipolar koter kullan›larak yap›lan unilateral proksimal tubal oklüzyon iflleminin birinci ve alt›nc› aylarda rat over histopatolojisi üzerine etkilerinin incelenmesi. Yöntem: Eriflkin 28 Wistar albino rat östrus faz›nda rastgele 4 gruba ayr›ld›. G1 (n=7): Bat›n aç›l›p kapat›lan ve 1 ay sonra sol ooferektomi yap›lan grup. G2 (n=7): Sol proksimal tubal oklüzyon yap›l›p 1 ay sonra sol ooferektomi yap›lan grup. G3 (n=7): Bat›n aç›l›p kapat›lan ve 6 ay sonra sol ooferektomi yap›lan grup. G4 (n=7): Sol proksimal tubal oklüzyon yap›l›p 6 ay sonra sol ooferektomi yap›lan grup. Sol over örnekleri formaldehitle tespit edildi. Hematoksilen Eozin ile boyanan preparatlarda over folikül rezervi saptand›. Atretik foliküllerin say›s›, corpus luteum, corpus albicans tespit edildi. Corpus luteum içi anjiogenesiz varl›¤›ndaki gerileme ve ovaryan stromada fibrozis varl›¤› incelendi. Overdeki folikül kisti say›l-d›. G1-G2 ile G3-G4 ile karfl›laflt›r›ld›. Ordinal veriler için Mann Whitney U testi, nominal veriler için x 2 testleri kullan›ld›. p<0.05 anlaml› kabul edildi. Bulgular: G1-G2 ile, G3-G4 ile karfl›laflt›r›ld›¤›nda tüm de¤erler benzer bulundu (p>0.05, Mann Whitney U test). Corpus luteum içi anjiogenezisdeki gerileme G2 ve G4'de anlaml› olmamakla birlikte azalm›flt›. Hiçbir grupta makroskobik veya mikroskobik kist geliflmedi Sonuç: Ratlarda bipolar koter kullan›larak yap›lan unilateral proksimal tubal okluzyon ifllemi, erken ve geç dönemde over histopatolojisi üzerine herhangi bir zararl› etki yapmam›flt›r. Anahtar kelimeler: ovaryan histopatoloji, proksimal tubal oklüzyon, rat.
Clinical and Experimental Obstetrics & Gynecology, Aug 10, 2018
Objective: To examine the effects of lipiodol, melatonin, and radiation used during hysterosalpin... more Objective: To examine the effects of lipiodol, melatonin, and radiation used during hysterosalpingography (HSG) on rat ovarian follicle reserve. Materials and Methods: A total of 50 Wistar rats with regular estrous cycles were randomly divided into five groups. Group 1 was the control group. The other groups, X-ray was applied (G2), 0.1 ml lipiodol was applied to each uterine horn (G3), 20 mg/kg intraperitoneal melatonin application was followed by 0.1 ml lipiodol administration to each uterine horn after 15 minutes (G4), 20 mg/kg melatonin was administered to ligamentum suspensorium ovarii, followed by 0.1 ml lipiodol application to each uterine horn after 15 minutes (G5), respectively. The rats in G2, G3, G4, and G5 were exposed three times to whole body radiation. Then, all rats were re-opened and left oophorectomy was performed. Left ovarian samples were fixed in formaldehyde. Primordial, primary, secondary, and tertiary follicles were counted in the preparations, and all were added to calculate the ovarian follicle reserve. Results: Primordial, primary, and ovarian follicle reserves were found significantly lower in G2, in comparison to other groups. Atretic follicle values were significantly higher in G2 compared to G3, and in G3 compared to the other groups. Regression of angiogenesis within corpus luteum was found significantly lower in G2, compared to G3, and in G3 compared to other groups. All values in G1, G4, and G5 were similar. Conclusion: Use of lipiodol and melatonin during HSG procedure prevents the negative effects of radiation on ovarian follicle reserve.
ÖZET Amaç: %3, %50, %75 ve %95'lik konsantrasyonlardaki trikloroasetik asitin, endometriyal ablas... more ÖZET Amaç: %3, %50, %75 ve %95'lik konsantrasyonlardaki trikloroasetik asitin, endometriyal ablasyon için, atuşman ve instillasyon yöntemleri ile uygulanmasının rat over rezervi ile yüzey epitelindeki displazik değişiklikler üzerine etkisinin incelenmesi. Gereç ve Yöntem: 90 adet, Sprague Dawley rat, estrus fazında randomize blok düzenli (konsantrasyon karşılaştırmalı) çalışma programına alındı. Grup 1: (n=10): Batın açılıp kapatılan grup. Batın açılıp sol uterin horn distaline 2 cm'lik uzunlamasına insizyonu takiben, %3'lük TCA (Gruop 2, n=10), %50'lik TCA (Grup 3, n=10), %75'lik TCA (Grup 4, n=10) ve %95'lik TCA (Grup 5, n=10) atuşmanı yapılan grup. Batın açılıp sol uterin horna 0,1 ml %3'lük TCA (Grup 6, n=10), %50'lik TCA (Grup 7, n=10), %75'lik TCA (Grup 8, n=10) ve %95'lik TCA (Grup 9, n=10) instillasyonu yapılan grup. Tüm ratların 21 gün sonra estrus fazında batını açıldı. Sol ooferektomi yapıldı. Hematoksilen Eozin ile boyanan preparatlarda over folikül rezervi ve over yüzey epitel değişiklikleri değerlendirildi. Grup 2-Grup 6 ile, Grup 3-Grup 7 ile, Grup 4-Grup 8 ile, Grup 5-Grup 9 ile Mann Whitney U test ile karşılaştırıldı. Tüm grupların karşılaştırılması, Kruskall Wallis varyans analizi ile yapıldı. p<0.05 anlamlı kabul edildi. Bulgular: Over rezervi ve yüzey epitel değişiklikleri atuşman ve instillasyon grubunda benzer bulundu (p>0.05, Mann Whitney U test). Tüm grupların karşılaştırılmasında, over folikül rezervi ve yüzey epitel değişiklikleri benzer bulundu (p>0.05, Kruskall Wallis varyans analizi). Sonuç: Farklı konsantrasyonlardaki trikloroasetik asitin, endometriyal ablasyon için, atuşman ve instillasyon yöntemleri ile uygulanmasının rat over rezervi ve over yüzey epiteli üzerine olumsuz bir etkisi yoktur.
Objective: To examine the effects of melatonin use in the unilateral Pomeroy method of tubal liga... more Objective: To examine the effects of melatonin use in the unilateral Pomeroy method of tubal ligation on ovarian histology in rats. Setting: Firat University Medical School, Obstetrics and Gynecology Department, Elaziğ. Material and method: Thirty adult, female rats of Wistar albino species with regular cycles were randomly allocated to three groups in the estrus phase: G1 (n: 10): The abdomen was opened and closed. G2 (n: 10): The group where the abdomen was opened, and the Pomeroy method of tubal ligation was performed. G3 (n: 10): The group where the abdomen was opened, and Pomeroy method of tubal ligation was performed 15 min after 10 mg/kg/ip melatonin administration. Abdomens of all rats were opened six months later and left oophorectomy was performed. Samples of the left ovary were fixed in formaldehyde. The preparations were stained with hematoxylin eosin, and primordial, primary, secondary and tertiary follicles were counted. All the numbers were added up to determine the ovarian follicle reserve. An atretic follicle count was made. The corpus luteum and corpus albicans were counted, and the number of total corpuses were calculated. Regression of angiogenesis within the corpus luteum was examined. Presence of fibrosis on ovarian stroma was examined. An ordinal scale was formed for the regression of angiogenesis within the corpus luteum and presence of fibrosis (none: 0p, present: 1p, markedly present: 2). Follicle cysts in the ovary were counted. Kruskal Wallis variance analysis was used in the statistical analysis of data; p < 0.05 were considered significant. Results: The comparison between G1 and G3 showed that all values were similar (p > 0.05, Kruskal Wallis variance analysis). When G2 was compared with G1 and G3, regression of angiogenesis in the corpus luteum was found to be significantly lower (p < 0.05, Mann Whitney U test), while atretic follicle count and fibrosis were significantly higher in G2 (p < 0.05, Mann Whitney U test). Conclusion: The Pomeroy method of tubal ligation reduces regression of angiogenesis in the corpus luteum, and increases atretic follicles and fibrosis development. Melatonin use restores these harmful effects. Melatonin can be used to refrain from this negative effect of the Pomeroy method of tubal ligation on the ovary.
This experiment was conducted to determine the effect of vitamin E (dl-alpha-tocopherol acetate) ... more This experiment was conducted to determine the effect of vitamin E (dl-alpha-tocopherol acetate) on healing of radial diaphysis fracture in dogs. Eighteen adult (mean 3 -y-old), mongrel female dogs were divided into two equal groups. Fixation was performed with bone plate after fracture constituted on radial diaphysis. Postoperatively, dlalpha-tocopherol acetate (20 mg/kg/day) was injected intramuscularly to the first group (treatment group) for one week. The second group was kept as control group. The result of the study was evaluated clinical and radiographically on postoperative days of 15 th , 30 th and 60 th . At each examination time tissue samples of three cases of each group were harvested and these cases were freed. Dl-alpha-tocopherol acetate being more effective in early stages (first fifteen days) was evaluated due to its antioxidant effect on free oxygen radicals in the fracture region. Consequently, it was understood that dl-alpha-tocopherol acetate given for a week had a positive effect on the fracture healing in dogs in case of applying immediately (in early stages).
Medicine Science | International Medical Journal, 2016
In spite of the widespread use of non-steroidal anti-inflammatory drugs (NSAIDs) such as etodolac... more In spite of the widespread use of non-steroidal anti-inflammatory drugs (NSAIDs) such as etodolac, induction of drug-dependent antibodies that cause hemolytic anemia and acute tubulointerstitiel nephritis are rarely reported. Currently, there is no way of identifying individuals who are at risk to develop this kind of complications. In patients who present with acute immune hemolysis and acute renal failure, it is important that a careful history of medication be obtained, potential sensitizing medications be identified. In this article we describe a patient with acute autoimmune hemolytic anemia and acute renal failure related with etodolac.
Fibrous hamartoma (FH) is a focal malformation resembling a neoplasm, composed of an overgrowth o... more Fibrous hamartoma (FH) is a focal malformation resembling a neoplasm, composed of an overgrowth of mature cells and tissues that normally occur in the affected area. But they are characterized by disorganization. FHs are usually seen at childhood and located in head and neck region, gastrointestinal system and lung. It is not reported previously in the paratesticular region and in adults. We reported a 47 years-old man with right testicular mass that was diagnosed as paratesticular fibrous hamartoma. In English literature, this is the first case of a fibrous hamartoma originating from paratesticular region in adults. We aim to discuss this case in light of the literature.
Simultaneous occurrence of the esophageal tumor at multiple sites in a single patient is unusual.... more Simultaneous occurrence of the esophageal tumor at multiple sites in a single patient is unusual. Here in, we described a patient had three separate tumoral nodules with ulceration of the esophagus. Thorax CT scan, X-ray and endoscopy revealed the only two tumoral lesion at the lower esophagus. But, the other tumoral lesion at the upper part of the esophagus was detected at operation and histopathologic examination. The aspect of treatment was changed according to this new condition during the operation. We discussed the multicentric tumoral lesions of the esophagus in view of the literature.
To investigate the efficacy of antibiotic-impregnated DuraGen® in preventing infection when used ... more To investigate the efficacy of antibiotic-impregnated DuraGen® in preventing infection when used for repair of dural defects. 2 groups of 7 samples were formed. In the first group, normal DuraGen® samples without antibiotic impregnation and in the second group, DuraGen® samples with antibiotic impregnation (30 mg vancomycin) were used. Staphylococcus aureus (ATCC 25923), and Staphylococcus epidermidis (ATCC 12228) were selected for this study. Antimicrobial efficacy was evaluated by counting the number of colonies on and by measuring the diameter of the inhibition zone around the samples. There was no zone diameter around the normal DuraGen® for the 2 types of bacteria. There was significant bacterial growth (10 (5)) according to the colony count. For antibiotic-impregnated DuraGen®, there was a zone with a mean diameter of 32 mm in the agar with S. aureus and a zone with a mean diameter of 35 mm in the agar with S. epidermidis. Antibiotic-impregnated DuraGen® was quite effective against both microorganisms and there was no bacterial growth according to the bacterial colony count. In our in vitro study, antibiotic-impregnated DuraGen® material significantly inhibited microorganism growth. Our results show that antibiotic-impregnated DuraGen® has the potential to prevent infection.
AIM: In this study, we aimed to review the diagnostic approach to eosinophilic cell renal neoplas... more AIM: In this study, we aimed to review the diagnostic approach to eosinophilic cell renal neoplasms by light microscopy and immunohistochemical techniques. METHOD: In this study 23 of these tumors were eosinophilic variant classic RCC, 15 eosinophilic variant papillary RCC, 13 eosinophilic variant chromophobe RCC and 13 oncocytoma cases. These tumors were immunohistochemically treated with CK7, CD117, EpCAM, Vimentin, RCCm (Renal cell carcinoma marker) and GST-α. RESULTS: In our study, contrary to the general literature on Vimentin, 65.2 % negativity was found in our patients with eosinophilic variant classic RCC. However, when compared with other tumor types in our study, vimentin expression was highest in eosinophilic variant classical RCC with 34.8 %. Statistically; RCCm, GST-α, EpCAM, CD117, CK7 were found to be signifi cantly associated with tumor types, while no signifi cant relationship was found between Vimentin and tumor types. RCCm positivity and CK7 and CD117 negativity were in favour of eosinophilic variant classical RCC, EpCAM, CK7 and CD117 positivity and Vimentin, GST-α and RCCm negativity supported eosinophilic variant chromophobe RCC, CK7 and RCCm positivity and CD117 and GST-α negativity were found in favour of eosinophilic variant papillary RCC. CD117 positivity and Vimentin, CK7 and GST-α negativity were found to support oncocytoma. CONCLUSIONS: The panel with RCCm, GST-α, EpCAM, CD117, CK7 will contribute to the differentiation of eosinophilic cytoplasm renal tumors that cannot be determined by morphological fi ndings and to reach the correct diagnosis (Tab. 3, Fig. 4, Ref. 54).
The aim of this study was to examine the effects of photoperiod on testes in rat. For this purpos... more The aim of this study was to examine the effects of photoperiod on testes in rat. For this purpose 21 male Wistar rats were used. Animals were divided into three groups. Control rats in group I were kept under 12 hrs light: 12 hrs dark conditions (12L: 12D) for 10 weeks. Animals in group II were exposed to long photoperiods (18L: 6D), while rats in group III were exposed to short photoperiods (6L: 18D) for 10 weeks. At the end of the experiment, all animals were killed by decapitation and blood samples were obtained. Serum testosterone levels were determined with the use of a chemiluminescent enzyme immunoassay. The testes of all rats were removed and weighed. Testicular tissue was processed semiquantitative evaluation of immunohistochemical testosterone staining. Intensity of immunostaining was determined on a scale between 0 (no staining) and 5 (heavy staining). For morphometric comparison, diameters of seminiferous tubules in each group were measured. In rats exposed to long photoperiods, testicular weights, diameters of seminiferous tubules and serum testosterone levels were signifi cantly increased as compared to those in control rats. Whereas, exposure of rats to short photoperiods resulted in signifi cantly decrease of testicular weights, diameters of seminiferous tubules and serum testosterone levels as compared to those in control rats and rats maintained in long photoperiods. Immunostaining of testosterone was moderate (3+) in control rats, heavy (5+) in rats exposed to long photoperiods and minimal (1+) in rats exposed to short photoperiods. The results of our study indicate that testicular functions increase after exposure to long photoperiods and decrease after exposure to short photoperiods.
... Azat ALINAK 1 , Tamer DEMİR 1 , Burak TURGUT 1 , Nusret AKPOLAT 2 , Orhan AYDEMİR 1 , Nesrin ... more ... Azat ALINAK 1 , Tamer DEMİR 1 , Burak TURGUT 1 , Nusret AKPOLAT 2 , Orhan AYDEMİR 1 , Nesrin DEMİR 3. ... Dokularda iskemi kritik bir zaman dilimini aşarsa; ardışık birtakım kimyasal olaylar oluşmakta ve bu olaylar hücre disfonksiyonu, interstisyel ödem, hücresel hasar ve ...
1st, 6th and 24th hour by visual analogue scale (VSA). Supplementary analgesic requirement and po... more 1st, 6th and 24th hour by visual analogue scale (VSA). Supplementary analgesic requirement and possible This present study investigated the effects of intracomplications were also followed. The intensity of articular morphine administration in 1 mg and 5 mg pain and analgesic requirement were reduced more doses on post-operative pain relief and analgesic rein the morphine 5 mg group than in the control group. quirements for patients undergoing arthroscopic pro-It is concluded, that the administration of intraarticular cedures. At the end of the operation patients were morphine 5 mg provides long-lasting and effective randomly allocated in a double-blinded fashion into analgesia after knee arthroscopy. three groups. The control group (Group 1) received normal saline 20 mL intraarticularly. The patients in
Alport syndrome is a type IV collagen synthesis disorder characterized by hereditary progressive ... more Alport syndrome is a type IV collagen synthesis disorder characterized by hereditary progressive glomerular disease resulting from glomerular basement membrane injury, often accompanied by hearing loss and ocular defects. The most common form is X-linked Alport syndrome, which accounts for 80% of all cases. Although women mainly present with mild urinary symptoms, end-stage renal disease onsets at an early age in men. An 11-year-old girl was admitted to our hospital with mild bifissure oedema for the last 3 months. This study discusses the clinical, morphological and transmission electron microscopic findings of a rare case of Alport syndrome in the context of the wider literature.
Bipolar koter kullan›larak yap›lan unilateral proksimal tubal oklüzyon iflleminin birinci ve alt›... more Bipolar koter kullan›larak yap›lan unilateral proksimal tubal oklüzyon iflleminin birinci ve alt›nc› aylarda rat over histopatolojisi üzerine etkilerinin incelenmesi. Yöntem: Eriflkin 28 Wistar albino rat östrus faz›nda rastgele 4 gruba ayr›ld›. G1 (n=7): Bat›n aç›l›p kapat›lan ve 1 ay sonra sol ooferektomi yap›lan grup. G2 (n=7): Sol proksimal tubal oklüzyon yap›l›p 1 ay sonra sol ooferektomi yap›lan grup. G3 (n=7): Bat›n aç›l›p kapat›lan ve 6 ay sonra sol ooferektomi yap›lan grup. G4 (n=7): Sol proksimal tubal oklüzyon yap›l›p 6 ay sonra sol ooferektomi yap›lan grup. Sol over örnekleri formaldehitle tespit edildi. Hematoksilen Eozin ile boyanan preparatlarda over folikül rezervi saptand›. Atretik foliküllerin say›s›, corpus luteum, corpus albicans tespit edildi. Corpus luteum içi anjiogenesiz varl›¤›ndaki gerileme ve ovaryan stromada fibrozis varl›¤› incelendi. Overdeki folikül kisti say›l-d›. G1-G2 ile G3-G4 ile karfl›laflt›r›ld›. Ordinal veriler için Mann Whitney U testi, nominal veriler için x 2 testleri kullan›ld›. p<0.05 anlaml› kabul edildi. Bulgular: G1-G2 ile, G3-G4 ile karfl›laflt›r›ld›¤›nda tüm de¤erler benzer bulundu (p>0.05, Mann Whitney U test). Corpus luteum içi anjiogenezisdeki gerileme G2 ve G4'de anlaml› olmamakla birlikte azalm›flt›. Hiçbir grupta makroskobik veya mikroskobik kist geliflmedi Sonuç: Ratlarda bipolar koter kullan›larak yap›lan unilateral proksimal tubal okluzyon ifllemi, erken ve geç dönemde over histopatolojisi üzerine herhangi bir zararl› etki yapmam›flt›r. Anahtar kelimeler: ovaryan histopatoloji, proksimal tubal oklüzyon, rat.
Clinical and Experimental Obstetrics & Gynecology, Aug 10, 2018
Objective: To examine the effects of lipiodol, melatonin, and radiation used during hysterosalpin... more Objective: To examine the effects of lipiodol, melatonin, and radiation used during hysterosalpingography (HSG) on rat ovarian follicle reserve. Materials and Methods: A total of 50 Wistar rats with regular estrous cycles were randomly divided into five groups. Group 1 was the control group. The other groups, X-ray was applied (G2), 0.1 ml lipiodol was applied to each uterine horn (G3), 20 mg/kg intraperitoneal melatonin application was followed by 0.1 ml lipiodol administration to each uterine horn after 15 minutes (G4), 20 mg/kg melatonin was administered to ligamentum suspensorium ovarii, followed by 0.1 ml lipiodol application to each uterine horn after 15 minutes (G5), respectively. The rats in G2, G3, G4, and G5 were exposed three times to whole body radiation. Then, all rats were re-opened and left oophorectomy was performed. Left ovarian samples were fixed in formaldehyde. Primordial, primary, secondary, and tertiary follicles were counted in the preparations, and all were added to calculate the ovarian follicle reserve. Results: Primordial, primary, and ovarian follicle reserves were found significantly lower in G2, in comparison to other groups. Atretic follicle values were significantly higher in G2 compared to G3, and in G3 compared to the other groups. Regression of angiogenesis within corpus luteum was found significantly lower in G2, compared to G3, and in G3 compared to other groups. All values in G1, G4, and G5 were similar. Conclusion: Use of lipiodol and melatonin during HSG procedure prevents the negative effects of radiation on ovarian follicle reserve.
ÖZET Amaç: %3, %50, %75 ve %95'lik konsantrasyonlardaki trikloroasetik asitin, endometriyal ablas... more ÖZET Amaç: %3, %50, %75 ve %95'lik konsantrasyonlardaki trikloroasetik asitin, endometriyal ablasyon için, atuşman ve instillasyon yöntemleri ile uygulanmasının rat over rezervi ile yüzey epitelindeki displazik değişiklikler üzerine etkisinin incelenmesi. Gereç ve Yöntem: 90 adet, Sprague Dawley rat, estrus fazında randomize blok düzenli (konsantrasyon karşılaştırmalı) çalışma programına alındı. Grup 1: (n=10): Batın açılıp kapatılan grup. Batın açılıp sol uterin horn distaline 2 cm'lik uzunlamasına insizyonu takiben, %3'lük TCA (Gruop 2, n=10), %50'lik TCA (Grup 3, n=10), %75'lik TCA (Grup 4, n=10) ve %95'lik TCA (Grup 5, n=10) atuşmanı yapılan grup. Batın açılıp sol uterin horna 0,1 ml %3'lük TCA (Grup 6, n=10), %50'lik TCA (Grup 7, n=10), %75'lik TCA (Grup 8, n=10) ve %95'lik TCA (Grup 9, n=10) instillasyonu yapılan grup. Tüm ratların 21 gün sonra estrus fazında batını açıldı. Sol ooferektomi yapıldı. Hematoksilen Eozin ile boyanan preparatlarda over folikül rezervi ve over yüzey epitel değişiklikleri değerlendirildi. Grup 2-Grup 6 ile, Grup 3-Grup 7 ile, Grup 4-Grup 8 ile, Grup 5-Grup 9 ile Mann Whitney U test ile karşılaştırıldı. Tüm grupların karşılaştırılması, Kruskall Wallis varyans analizi ile yapıldı. p<0.05 anlamlı kabul edildi. Bulgular: Over rezervi ve yüzey epitel değişiklikleri atuşman ve instillasyon grubunda benzer bulundu (p>0.05, Mann Whitney U test). Tüm grupların karşılaştırılmasında, over folikül rezervi ve yüzey epitel değişiklikleri benzer bulundu (p>0.05, Kruskall Wallis varyans analizi). Sonuç: Farklı konsantrasyonlardaki trikloroasetik asitin, endometriyal ablasyon için, atuşman ve instillasyon yöntemleri ile uygulanmasının rat over rezervi ve over yüzey epiteli üzerine olumsuz bir etkisi yoktur.
Objective: To examine the effects of melatonin use in the unilateral Pomeroy method of tubal liga... more Objective: To examine the effects of melatonin use in the unilateral Pomeroy method of tubal ligation on ovarian histology in rats. Setting: Firat University Medical School, Obstetrics and Gynecology Department, Elaziğ. Material and method: Thirty adult, female rats of Wistar albino species with regular cycles were randomly allocated to three groups in the estrus phase: G1 (n: 10): The abdomen was opened and closed. G2 (n: 10): The group where the abdomen was opened, and the Pomeroy method of tubal ligation was performed. G3 (n: 10): The group where the abdomen was opened, and Pomeroy method of tubal ligation was performed 15 min after 10 mg/kg/ip melatonin administration. Abdomens of all rats were opened six months later and left oophorectomy was performed. Samples of the left ovary were fixed in formaldehyde. The preparations were stained with hematoxylin eosin, and primordial, primary, secondary and tertiary follicles were counted. All the numbers were added up to determine the ovarian follicle reserve. An atretic follicle count was made. The corpus luteum and corpus albicans were counted, and the number of total corpuses were calculated. Regression of angiogenesis within the corpus luteum was examined. Presence of fibrosis on ovarian stroma was examined. An ordinal scale was formed for the regression of angiogenesis within the corpus luteum and presence of fibrosis (none: 0p, present: 1p, markedly present: 2). Follicle cysts in the ovary were counted. Kruskal Wallis variance analysis was used in the statistical analysis of data; p < 0.05 were considered significant. Results: The comparison between G1 and G3 showed that all values were similar (p > 0.05, Kruskal Wallis variance analysis). When G2 was compared with G1 and G3, regression of angiogenesis in the corpus luteum was found to be significantly lower (p < 0.05, Mann Whitney U test), while atretic follicle count and fibrosis were significantly higher in G2 (p < 0.05, Mann Whitney U test). Conclusion: The Pomeroy method of tubal ligation reduces regression of angiogenesis in the corpus luteum, and increases atretic follicles and fibrosis development. Melatonin use restores these harmful effects. Melatonin can be used to refrain from this negative effect of the Pomeroy method of tubal ligation on the ovary.
This experiment was conducted to determine the effect of vitamin E (dl-alpha-tocopherol acetate) ... more This experiment was conducted to determine the effect of vitamin E (dl-alpha-tocopherol acetate) on healing of radial diaphysis fracture in dogs. Eighteen adult (mean 3 -y-old), mongrel female dogs were divided into two equal groups. Fixation was performed with bone plate after fracture constituted on radial diaphysis. Postoperatively, dlalpha-tocopherol acetate (20 mg/kg/day) was injected intramuscularly to the first group (treatment group) for one week. The second group was kept as control group. The result of the study was evaluated clinical and radiographically on postoperative days of 15 th , 30 th and 60 th . At each examination time tissue samples of three cases of each group were harvested and these cases were freed. Dl-alpha-tocopherol acetate being more effective in early stages (first fifteen days) was evaluated due to its antioxidant effect on free oxygen radicals in the fracture region. Consequently, it was understood that dl-alpha-tocopherol acetate given for a week had a positive effect on the fracture healing in dogs in case of applying immediately (in early stages).
Medicine Science | International Medical Journal, 2016
In spite of the widespread use of non-steroidal anti-inflammatory drugs (NSAIDs) such as etodolac... more In spite of the widespread use of non-steroidal anti-inflammatory drugs (NSAIDs) such as etodolac, induction of drug-dependent antibodies that cause hemolytic anemia and acute tubulointerstitiel nephritis are rarely reported. Currently, there is no way of identifying individuals who are at risk to develop this kind of complications. In patients who present with acute immune hemolysis and acute renal failure, it is important that a careful history of medication be obtained, potential sensitizing medications be identified. In this article we describe a patient with acute autoimmune hemolytic anemia and acute renal failure related with etodolac.
Fibrous hamartoma (FH) is a focal malformation resembling a neoplasm, composed of an overgrowth o... more Fibrous hamartoma (FH) is a focal malformation resembling a neoplasm, composed of an overgrowth of mature cells and tissues that normally occur in the affected area. But they are characterized by disorganization. FHs are usually seen at childhood and located in head and neck region, gastrointestinal system and lung. It is not reported previously in the paratesticular region and in adults. We reported a 47 years-old man with right testicular mass that was diagnosed as paratesticular fibrous hamartoma. In English literature, this is the first case of a fibrous hamartoma originating from paratesticular region in adults. We aim to discuss this case in light of the literature.
Simultaneous occurrence of the esophageal tumor at multiple sites in a single patient is unusual.... more Simultaneous occurrence of the esophageal tumor at multiple sites in a single patient is unusual. Here in, we described a patient had three separate tumoral nodules with ulceration of the esophagus. Thorax CT scan, X-ray and endoscopy revealed the only two tumoral lesion at the lower esophagus. But, the other tumoral lesion at the upper part of the esophagus was detected at operation and histopathologic examination. The aspect of treatment was changed according to this new condition during the operation. We discussed the multicentric tumoral lesions of the esophagus in view of the literature.
To investigate the efficacy of antibiotic-impregnated DuraGen® in preventing infection when used ... more To investigate the efficacy of antibiotic-impregnated DuraGen® in preventing infection when used for repair of dural defects. 2 groups of 7 samples were formed. In the first group, normal DuraGen® samples without antibiotic impregnation and in the second group, DuraGen® samples with antibiotic impregnation (30 mg vancomycin) were used. Staphylococcus aureus (ATCC 25923), and Staphylococcus epidermidis (ATCC 12228) were selected for this study. Antimicrobial efficacy was evaluated by counting the number of colonies on and by measuring the diameter of the inhibition zone around the samples. There was no zone diameter around the normal DuraGen® for the 2 types of bacteria. There was significant bacterial growth (10 (5)) according to the colony count. For antibiotic-impregnated DuraGen®, there was a zone with a mean diameter of 32 mm in the agar with S. aureus and a zone with a mean diameter of 35 mm in the agar with S. epidermidis. Antibiotic-impregnated DuraGen® was quite effective against both microorganisms and there was no bacterial growth according to the bacterial colony count. In our in vitro study, antibiotic-impregnated DuraGen® material significantly inhibited microorganism growth. Our results show that antibiotic-impregnated DuraGen® has the potential to prevent infection.
AIM: In this study, we aimed to review the diagnostic approach to eosinophilic cell renal neoplas... more AIM: In this study, we aimed to review the diagnostic approach to eosinophilic cell renal neoplasms by light microscopy and immunohistochemical techniques. METHOD: In this study 23 of these tumors were eosinophilic variant classic RCC, 15 eosinophilic variant papillary RCC, 13 eosinophilic variant chromophobe RCC and 13 oncocytoma cases. These tumors were immunohistochemically treated with CK7, CD117, EpCAM, Vimentin, RCCm (Renal cell carcinoma marker) and GST-α. RESULTS: In our study, contrary to the general literature on Vimentin, 65.2 % negativity was found in our patients with eosinophilic variant classic RCC. However, when compared with other tumor types in our study, vimentin expression was highest in eosinophilic variant classical RCC with 34.8 %. Statistically; RCCm, GST-α, EpCAM, CD117, CK7 were found to be signifi cantly associated with tumor types, while no signifi cant relationship was found between Vimentin and tumor types. RCCm positivity and CK7 and CD117 negativity were in favour of eosinophilic variant classical RCC, EpCAM, CK7 and CD117 positivity and Vimentin, GST-α and RCCm negativity supported eosinophilic variant chromophobe RCC, CK7 and RCCm positivity and CD117 and GST-α negativity were found in favour of eosinophilic variant papillary RCC. CD117 positivity and Vimentin, CK7 and GST-α negativity were found to support oncocytoma. CONCLUSIONS: The panel with RCCm, GST-α, EpCAM, CD117, CK7 will contribute to the differentiation of eosinophilic cytoplasm renal tumors that cannot be determined by morphological fi ndings and to reach the correct diagnosis (Tab. 3, Fig. 4, Ref. 54).
The aim of this study was to examine the effects of photoperiod on testes in rat. For this purpos... more The aim of this study was to examine the effects of photoperiod on testes in rat. For this purpose 21 male Wistar rats were used. Animals were divided into three groups. Control rats in group I were kept under 12 hrs light: 12 hrs dark conditions (12L: 12D) for 10 weeks. Animals in group II were exposed to long photoperiods (18L: 6D), while rats in group III were exposed to short photoperiods (6L: 18D) for 10 weeks. At the end of the experiment, all animals were killed by decapitation and blood samples were obtained. Serum testosterone levels were determined with the use of a chemiluminescent enzyme immunoassay. The testes of all rats were removed and weighed. Testicular tissue was processed semiquantitative evaluation of immunohistochemical testosterone staining. Intensity of immunostaining was determined on a scale between 0 (no staining) and 5 (heavy staining). For morphometric comparison, diameters of seminiferous tubules in each group were measured. In rats exposed to long photoperiods, testicular weights, diameters of seminiferous tubules and serum testosterone levels were signifi cantly increased as compared to those in control rats. Whereas, exposure of rats to short photoperiods resulted in signifi cantly decrease of testicular weights, diameters of seminiferous tubules and serum testosterone levels as compared to those in control rats and rats maintained in long photoperiods. Immunostaining of testosterone was moderate (3+) in control rats, heavy (5+) in rats exposed to long photoperiods and minimal (1+) in rats exposed to short photoperiods. The results of our study indicate that testicular functions increase after exposure to long photoperiods and decrease after exposure to short photoperiods.
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