Jimmy John

Maharshi Dayanand College, University Of Mumbai, Botany, Assistant Professor (CHB)

Vellore Institute of Technology, Biotechnology, PhD student

SIES College, University of Mumbai, Botany, Graduate Student

Followers

Following

Public Views

Jimmy John Lilly graduated with PhD from Vellore Institute of technology. His research interests lies with plant-microbe interactions and crop improvement. His doctoral research revolves around the WRKY13 Transcription Factor gene and its role in rice disease resistance against two sheath infecting fungal pathogens. Interested in collaborating over a project and visiting positions.
Phone: 091-9167032804

less

VIT University

Ferdowsi University of Mashhad

Vívian Viana

Vishesh Kumar

National Research Centre on Plant Biotechnology

Amolkumar Solanke

Balkrishna Shinde

InterestsView All (10)

Uploads

Papers by Jimmy John

Publication 1 Jimmy

Download

Rice WRKY13 TF protein binds to motifs in the promoter region to regulate downstream disease resistance-related genes

Functional & Integrative Genomics, 2023

In plants, pathogen resistance is brought about by the binding of certain transcription factor (T... more In plants, pathogen resistance is brought about by the binding of certain transcription factor (TF) proteins to the cis-elements of certain target genes. These cis-elements are present upstream in the motif of the promoters of each gene. This ensures the binding of a specific TF to a specific promoter, therefore regulating the expression of that gene. Therefore, the study of each promoter sequence of all the rice genes would help identify the target genes of a specific TF. Rice 1 kb upstream promoter sequences of 55,986 annotated genes were analyzed using the Perl program algorithm to detect WRKY13 binding motifs (bm). The resulting genes were grouped using Gene Ontology and gene set enrichment analysis. A gene with more than 4 TF bm in their promoter was selected. Ten genes reported to have a role in rice disease resistance were selected for further analysis. Cis-acting regulatory element analysis was carried out to find the cis-elements and confirm the presence of the corresponding motifs in the promoter sequences of these genes. The 3D structure of WRKY13 TF and the corresponding ten genes were built, and the interacting residues were determined. The binding capacity of WRKY13 to the promoter of these selected genes was analyzed using docking studies. WRKY13 was considered for docking analysis based on the prior reports of autoregulation. Molecular dynamic simulations provided more details regarding the interactions. Expression data revealed the expression of the genes that helped provide the mechanism of interaction. Further co-expression network helped to characterize the interaction of these selected disease resistance-related genes with the WRKY13 TF protein. This study suggests downstream target genes that are regulated by the WRKY13 TF. The molecular mechanism involving the gene network regulated by WRKY13 TF in disease resistance against rice fungal pathogens is explored.

Download

Publication 1 Jimmy

Download

Rice WRKY13 TF protein binds to motifs in the promoter region to regulate downstream disease resistance-related genes

Functional & Integrative Genomics, 2023

Download

Jimmy John

Uploads

Papers by Jimmy John

Log In