The purpose of this study was to determine the serological prevalence of bovine leukemia virus (B... more The purpose of this study was to determine the serological prevalence of bovine leukemia virus (BLV), and describe whether there are effects of BLV infection on the fertility of dairy cattle in Burdur, Turkey. The study population was 182 clinically healthy Holstein cows (>2-years-old) and 81 heifers (17- to 20-months-old) from a commercial dairy herd in Burdur. BLV prevalence was 66.48% (121/182) and 62.96% (51/81) in cows and heifers, respectively. There were no significant differences between BLV antibody-negative and antibody--positive cows for days open (DO; P = 0.2567) or between BLV antibody-negative and antibody-positive heifers for first service age (FSA; P = 0.65). Moreover, differences in conception rates (CR) between BLV antibody-negative and antibody-positive cows or heifers were insignificant (P > 0.1). In conclusion, even though the prevalence of BLV infection in Burdur region was found to be high, no effect of BLV infection was detected for fertility. Keywords: bovine leukemia, dairy cows
The aim of the present study was to determine the possible relationship between bovine viral diar... more The aim of the present study was to determine the possible relationship between bovine viral diarrhoea (BVD) virus infection and the appearance of cervical mucous discharge (CMD) and the reproductive performance of cows in oestrus. For this purpose, CMD from 97 Holstein cows in oestrus was evaluated visually before artificial insemination (AI). Cows in oestrus were inseminated with frozen semen free from BVD virus (BVDV). Blood samples were tested by enzyme-linked immunoassay (ELISA) for antigen (Ag) and antibodies (Ab) of BVDV. The presence of the BVDV genome in cervical mucus samples was tested by reverse transcriptase-polymerase chain reaction (RT-PCR). The presence of BVDV Ab, Ag or genome was not associated with abnormal cervical mucous discharge (A-CMD). However, the presence of BVDV Ag (but not of the BVDV Ab) in blood samples was associated with a lower first service conception rate (FSCR; 27.8 vs. 70.9%; P < 0.01), indicating that BVDV viraemia at the time of AI has a negative effect on the fertility of cows.
ÖZET: Bu çalışma ile Gonadotrophin Releasing Hormon&amp;#x27;un erken post partum dönemde, sü... more ÖZET: Bu çalışma ile Gonadotrophin Releasing Hormon&amp;#x27;un erken post partum dönemde, sütçü ineklerde kullanılmasının döl verimi üzerine etkisi araştırılmıştır. Araştırmada bakım ve barınma koşulları yeterli olan post partum dönemdeki 237 inek kullanılmıştır. Bu inekler iki ...
This study has been carried out in the Western Mediterranean Region of Turkey on seven different ... more This study has been carried out in the Western Mediterranean Region of Turkey on seven different Honamli goat flocks. The aim of this study was to determine the growth characters, survival rate (till weaning, 105th day), some reproductive characteristics and marketable milk yield. The data were collected from 516 does and 210 kids (125 buckling and 85 doelings). The results indicate that the numbers of multiple birth and litter size were 32.8 % and 1.35 respectively. Survival rates of kids at the 30th, 90th, and 105th days were 98.5, 97.2, and 97.2 %, respectively. The average birth weight for the buckling and doelings was 4.1 and 3.7 kg, respectively. The average weaning weights for buckling and doelings were 26.9 and 23.4 kg, respectively. The effects of dams' age, on sex and birth type on the growth of Honamli kids were statistically significant (p<0.05, p<0.01, and p<0.001). While the diameters of erythrocytes of Honamli goats are smaller, the density of hemoglobin in the erythrocytes is more than Asian, Indian, and American caprine breeds. The average lactation length and marketable milk yield was assessed to be at 210.3 days and was 89 L, respectively. The growth characteristics of Honamli kids at weaning age were assessed to be higher than most of the other goat breeds in Turkey. The results further indicated that there is a significant variation in the production traits among the different Honamli goat flocks. Production traits of Honamli goats at different breeding conditions should further be investigated to come to a definite conclusion.
The aim of the present study was to describe whether abnormal cervical mucus discharge (A-CMD) or... more The aim of the present study was to describe whether abnormal cervical mucus discharge (A-CMD) or pathogens in cervical mucus discharge (CMD) have effects on reproductive performance of cows and heifers in estrus. Animals having clear discharges (68 cows, 38 heifers) with normal viscosity and without bad odor were grouped as normal cervical mucous discharge (N-CMD) group. The other animals (84 cows, 32 heifers) were grouped as A-CMD group. Microorganisms isolated from samples were divided into three groups as uterine pathogens (UP), potential uterine pathogens (PUP) or opportunistic uterine pathogens (OUP). Presence of PUP was associated with A-CMD for both cows (p<0.01) and heifers (p<0.02). First service conception rates (FS-CR) were lower in cows positive for PUP (p<0.01). Moreover, presence of PUP and OUP affected FS-CR in heifers (p<0.01). Although A-CMD significantly affected FS-CR in cows (p<0.04), it did not affect FS-CR in heifers. Differences in average open day for cows (p<0.02) and first service age for heifers (p<0.01) were significant between N-CMD and A-CMD groups, respectively. The current study suggested that CMD should be evaluated more carefully when there are infertility problems. In addition to the known microorganism that causes sterility and infertility in the UP group, pathogens in the PUP group should be considered for their potential to cause infertility. (
We have previously shown that surfactant protein (SP)-A and SP-D are present in the stallion's ge... more We have previously shown that surfactant protein (SP)-A and SP-D are present in the stallion's genital system. The aim of the present study was to evaluate the expression and presence of SP-A and SP-D in the seminal plasma and accessory sex glands of stallions. We characterized further the SP-A and SP-D in the seminal plasma of stallions by western blotting and immunohistochemically in accessory sex glands. On one-dimensional SDS-PAGE electrophoresis and western blots, bands for SP-A (34 kDa) and SP-D (43 kDa) in seminal plasma and accessory sex gland tissue extracts were detected. The similarity between biochemical properties (relative molecular masses) of SP-A and SP-D in accessory sex glands and seminal plasma imply that SP-A and SP-D proteins are also present in the seminal plasma and accessory sex glands of stallions.
The aim of the study was to characterize bovine viral diarrhoea virus (BVDV) infection in pregnan... more The aim of the study was to characterize bovine viral diarrhoea virus (BVDV) infection in pregnant cows and their foetuses. Blood samples from 64 clinically healthy cows, determined as pregnant after slaughter, and from their foetuses were collected into tubes ...
Objective: To investigate the presence of surfactant protein-A (SP-A); molecular weight 34 kDa an... more Objective: To investigate the presence of surfactant protein-A (SP-A); molecular weight 34 kDa and surfactant protein-D (SP-D); and molecular weight 43 kDa in human spermatozoa. Design: Prospective, research study. Setting: Two universities in Turkey. Patient(s): Semen specimens (n ¼ 10) were obtained from normozoospermic donors. Intervention(s): Human sperm were exposed to an anti-human SP-A polyclonal antibody, and monoclonal antibody, to human SP-D protein. Main Outcome Measure(s): Presence of SP-A and SP-D proteins in human beings. Result(s): Indirect immunofluorescence assays of human sperm indicated the presence of SP-A in the mid piece, the tail, and sometimes at the equatorial region of spermatozoa. A brilliant green light detected SP-D in the tails and acrosome of some sperm. The anti-SP-A antibody detected a single band corresponding to the molecular weight values of 34 kDa in spermatozoa, whereas no band was observed in the negative control. The anti-SP-D antibody showed the expected band at 43 kDa in spermatozoa. Conclusion(s): This is the first report and a novel finding of the presence of surfactant glycoproteins on human spermatozoa.
The presence of surface-active material in the lung alveolus has been known for several decades a... more The presence of surface-active material in the lung alveolus has been known for several decades as being essential for normal lung function. The host defense and controlling inflammatory processes of the lung are the major functions of SP-A and SP-D.
The presence of surface-active material in the lung alveolus has been known for several decades a... more The presence of surface-active material in the lung alveolus has been known for several decades as being essential for normal lung function. Surfactant is essential for reducing the surface tension at the alveolar air-liquid interface. Pulmonary surfactant is composed of 90% lipids and 10% proteins. There are four non-serum proteins surfactant protein-A (SP-A), surfactant protein-B (SP-B), surfactant protein-C (SP-C) and surfactant protein-D (SP-D) named in chronologic order of discovery. Lung SP-A and SP-D belong to a family of collagen-containing C-type lectin family called collectins. The host defence and controlling inflammatory processes of the lung are the major functions of SP-A and SP-D.
The purpose of this study was to determine the serological prevalence of bovine leukemia virus (B... more The purpose of this study was to determine the serological prevalence of bovine leukemia virus (BLV), and describe whether there are effects of BLV infection on the fertility of dairy cattle in Burdur, Turkey. The study population was 182 clinically healthy Holstein cows (>2-years-old) and 81 heifers (17- to 20-months-old) from a commercial dairy herd in Burdur. BLV prevalence was 66.48% (121/182) and 62.96% (51/81) in cows and heifers, respectively. There were no significant differences between BLV antibody-negative and antibody--positive cows for days open (DO; P = 0.2567) or between BLV antibody-negative and antibody-positive heifers for first service age (FSA; P = 0.65). Moreover, differences in conception rates (CR) between BLV antibody-negative and antibody-positive cows or heifers were insignificant (P > 0.1). In conclusion, even though the prevalence of BLV infection in Burdur region was found to be high, no effect of BLV infection was detected for fertility. Keywords: bovine leukemia, dairy cows
The aim of the present study was to determine the possible relationship between bovine viral diar... more The aim of the present study was to determine the possible relationship between bovine viral diarrhoea (BVD) virus infection and the appearance of cervical mucous discharge (CMD) and the reproductive performance of cows in oestrus. For this purpose, CMD from 97 Holstein cows in oestrus was evaluated visually before artificial insemination (AI). Cows in oestrus were inseminated with frozen semen free from BVD virus (BVDV). Blood samples were tested by enzyme-linked immunoassay (ELISA) for antigen (Ag) and antibodies (Ab) of BVDV. The presence of the BVDV genome in cervical mucus samples was tested by reverse transcriptase-polymerase chain reaction (RT-PCR). The presence of BVDV Ab, Ag or genome was not associated with abnormal cervical mucous discharge (A-CMD). However, the presence of BVDV Ag (but not of the BVDV Ab) in blood samples was associated with a lower first service conception rate (FSCR; 27.8 vs. 70.9%; P < 0.01), indicating that BVDV viraemia at the time of AI has a negative effect on the fertility of cows.
ÖZET: Bu çalışma ile Gonadotrophin Releasing Hormon&amp;#x27;un erken post partum dönemde, sü... more ÖZET: Bu çalışma ile Gonadotrophin Releasing Hormon&amp;#x27;un erken post partum dönemde, sütçü ineklerde kullanılmasının döl verimi üzerine etkisi araştırılmıştır. Araştırmada bakım ve barınma koşulları yeterli olan post partum dönemdeki 237 inek kullanılmıştır. Bu inekler iki ...
This study has been carried out in the Western Mediterranean Region of Turkey on seven different ... more This study has been carried out in the Western Mediterranean Region of Turkey on seven different Honamli goat flocks. The aim of this study was to determine the growth characters, survival rate (till weaning, 105th day), some reproductive characteristics and marketable milk yield. The data were collected from 516 does and 210 kids (125 buckling and 85 doelings). The results indicate that the numbers of multiple birth and litter size were 32.8 % and 1.35 respectively. Survival rates of kids at the 30th, 90th, and 105th days were 98.5, 97.2, and 97.2 %, respectively. The average birth weight for the buckling and doelings was 4.1 and 3.7 kg, respectively. The average weaning weights for buckling and doelings were 26.9 and 23.4 kg, respectively. The effects of dams' age, on sex and birth type on the growth of Honamli kids were statistically significant (p<0.05, p<0.01, and p<0.001). While the diameters of erythrocytes of Honamli goats are smaller, the density of hemoglobin in the erythrocytes is more than Asian, Indian, and American caprine breeds. The average lactation length and marketable milk yield was assessed to be at 210.3 days and was 89 L, respectively. The growth characteristics of Honamli kids at weaning age were assessed to be higher than most of the other goat breeds in Turkey. The results further indicated that there is a significant variation in the production traits among the different Honamli goat flocks. Production traits of Honamli goats at different breeding conditions should further be investigated to come to a definite conclusion.
The aim of the present study was to describe whether abnormal cervical mucus discharge (A-CMD) or... more The aim of the present study was to describe whether abnormal cervical mucus discharge (A-CMD) or pathogens in cervical mucus discharge (CMD) have effects on reproductive performance of cows and heifers in estrus. Animals having clear discharges (68 cows, 38 heifers) with normal viscosity and without bad odor were grouped as normal cervical mucous discharge (N-CMD) group. The other animals (84 cows, 32 heifers) were grouped as A-CMD group. Microorganisms isolated from samples were divided into three groups as uterine pathogens (UP), potential uterine pathogens (PUP) or opportunistic uterine pathogens (OUP). Presence of PUP was associated with A-CMD for both cows (p<0.01) and heifers (p<0.02). First service conception rates (FS-CR) were lower in cows positive for PUP (p<0.01). Moreover, presence of PUP and OUP affected FS-CR in heifers (p<0.01). Although A-CMD significantly affected FS-CR in cows (p<0.04), it did not affect FS-CR in heifers. Differences in average open day for cows (p<0.02) and first service age for heifers (p<0.01) were significant between N-CMD and A-CMD groups, respectively. The current study suggested that CMD should be evaluated more carefully when there are infertility problems. In addition to the known microorganism that causes sterility and infertility in the UP group, pathogens in the PUP group should be considered for their potential to cause infertility. (
We have previously shown that surfactant protein (SP)-A and SP-D are present in the stallion's ge... more We have previously shown that surfactant protein (SP)-A and SP-D are present in the stallion's genital system. The aim of the present study was to evaluate the expression and presence of SP-A and SP-D in the seminal plasma and accessory sex glands of stallions. We characterized further the SP-A and SP-D in the seminal plasma of stallions by western blotting and immunohistochemically in accessory sex glands. On one-dimensional SDS-PAGE electrophoresis and western blots, bands for SP-A (34 kDa) and SP-D (43 kDa) in seminal plasma and accessory sex gland tissue extracts were detected. The similarity between biochemical properties (relative molecular masses) of SP-A and SP-D in accessory sex glands and seminal plasma imply that SP-A and SP-D proteins are also present in the seminal plasma and accessory sex glands of stallions.
The aim of the study was to characterize bovine viral diarrhoea virus (BVDV) infection in pregnan... more The aim of the study was to characterize bovine viral diarrhoea virus (BVDV) infection in pregnant cows and their foetuses. Blood samples from 64 clinically healthy cows, determined as pregnant after slaughter, and from their foetuses were collected into tubes ...
Objective: To investigate the presence of surfactant protein-A (SP-A); molecular weight 34 kDa an... more Objective: To investigate the presence of surfactant protein-A (SP-A); molecular weight 34 kDa and surfactant protein-D (SP-D); and molecular weight 43 kDa in human spermatozoa. Design: Prospective, research study. Setting: Two universities in Turkey. Patient(s): Semen specimens (n ¼ 10) were obtained from normozoospermic donors. Intervention(s): Human sperm were exposed to an anti-human SP-A polyclonal antibody, and monoclonal antibody, to human SP-D protein. Main Outcome Measure(s): Presence of SP-A and SP-D proteins in human beings. Result(s): Indirect immunofluorescence assays of human sperm indicated the presence of SP-A in the mid piece, the tail, and sometimes at the equatorial region of spermatozoa. A brilliant green light detected SP-D in the tails and acrosome of some sperm. The anti-SP-A antibody detected a single band corresponding to the molecular weight values of 34 kDa in spermatozoa, whereas no band was observed in the negative control. The anti-SP-D antibody showed the expected band at 43 kDa in spermatozoa. Conclusion(s): This is the first report and a novel finding of the presence of surfactant glycoproteins on human spermatozoa.
The presence of surface-active material in the lung alveolus has been known for several decades a... more The presence of surface-active material in the lung alveolus has been known for several decades as being essential for normal lung function. The host defense and controlling inflammatory processes of the lung are the major functions of SP-A and SP-D.
The presence of surface-active material in the lung alveolus has been known for several decades a... more The presence of surface-active material in the lung alveolus has been known for several decades as being essential for normal lung function. Surfactant is essential for reducing the surface tension at the alveolar air-liquid interface. Pulmonary surfactant is composed of 90% lipids and 10% proteins. There are four non-serum proteins surfactant protein-A (SP-A), surfactant protein-B (SP-B), surfactant protein-C (SP-C) and surfactant protein-D (SP-D) named in chronologic order of discovery. Lung SP-A and SP-D belong to a family of collagen-containing C-type lectin family called collectins. The host defence and controlling inflammatory processes of the lung are the major functions of SP-A and SP-D.
Uploads
Papers by Ayhan Ata
population was 182 clinically healthy Holstein cows (>2-years-old) and 81 heifers (17- to 20-months-old) from a commercial dairy herd in Burdur. BLV prevalence was 66.48% (121/182) and 62.96% (51/81) in cows and heifers, respectively. There were no significant differences between BLV antibody-negative and antibody--positive cows for days open (DO; P = 0.2567) or between BLV antibody-negative and antibody-positive heifers for first service age (FSA; P = 0.65). Moreover, differences in conception rates (CR) between BLV antibody-negative and antibody-positive cows or heifers were insignificant (P > 0.1). In conclusion, even though the prevalence of BLV infection in Burdur region was found to be high, no effect of BLV infection was detected for fertility.
Keywords: bovine leukemia, dairy cows
population was 182 clinically healthy Holstein cows (>2-years-old) and 81 heifers (17- to 20-months-old) from a commercial dairy herd in Burdur. BLV prevalence was 66.48% (121/182) and 62.96% (51/81) in cows and heifers, respectively. There were no significant differences between BLV antibody-negative and antibody--positive cows for days open (DO; P = 0.2567) or between BLV antibody-negative and antibody-positive heifers for first service age (FSA; P = 0.65). Moreover, differences in conception rates (CR) between BLV antibody-negative and antibody-positive cows or heifers were insignificant (P > 0.1). In conclusion, even though the prevalence of BLV infection in Burdur region was found to be high, no effect of BLV infection was detected for fertility.
Keywords: bovine leukemia, dairy cows