Nanostructures were fabricated using laser irradiating at the conductive tip of an atomic force m... more Nanostructures were fabricated using laser irradiating at the conductive tip of an atomic force microscope (AFM). The conductive tip behaves like an antenna, which receives electromagnetic energy from the laser beam and then emits electromagnetic wave in nanometer range. In this study, a commercial atomic force microscope, combined with a 514.5 nm argon ion laser and a vertically-polarized 532 nm Nd:YAG pulsed laser, was utilized for modification on the gold and 1813 photo-resist films. AFM imaging and nanostructure modification were conducted at the contact-constant-height mode. AFM images were obtained right after the laser irradiations to monitor the modification process. The effect of the laser intensity, the optical field enhancement, and the thermal expansion of the tip under laser irradiation are investigated.Nanostructures were fabricated using laser irradiating at the conductive tip of an atomic force microscope (AFM). The conductive tip behaves like an antenna, which receives electromagnetic energy from the laser beam and then emits electromagnetic wave in nanometer range. In this study, a commercial atomic force microscope, combined with a 514.5 nm argon ion laser and a vertically-polarized 532 nm Nd:YAG pulsed laser, was utilized for modification on the gold and 1813 photo-resist films. AFM imaging and nanostructure modification were conducted at the contact-constant-height mode. AFM images were obtained right after the laser irradiations to monitor the modification process. The effect of the laser intensity, the optical field enhancement, and the thermal expansion of the tip under laser irradiation are investigated.
Opto-Electronics and Communications Conference, Jul 4, 2011
We developed a glucose fiber sensor for measuring the glucose concentration in serum with heterod... more We developed a glucose fiber sensor for measuring the glucose concentration in serum with heterodyne interferometry. The best response time and the resolutions of the fiber sensor are 1.3 seconds and 0.152 mg/dl, respectively. Besides, the fiber sensor can be reused more than 10 times.
The concentration and pH value of the immobilized glucose oxidase (GOD) are critical parameters i... more The concentration and pH value of the immobilized glucose oxidase (GOD) are critical parameters in a glucose sensor. In this study, we develop a glucose fiber sensor integrated with heterodyne interferometry to measure the phase difference arising from the chemical reaction between glucose and GOD. Our studies show that the response time and resolution of this sensor will be strongly affected by the pH and concentration properties of GOD. In addition, the results show good linearity between the calibration curve for the glucose solution and serum based sample. The response time of this sensor can be shorter than 3 seconds and best resolutions are 0.1 and 0.136 mg/dl for glucose solution and serum based sample, respectively.
Using in vivo multiphoton fluorescent dosimetry, we demonstrate that the clearance dynamics of In... more Using in vivo multiphoton fluorescent dosimetry, we demonstrate that the clearance dynamics of Indocyanine Green (ICG) in the blood can quickly reveal liver function reserve. In normal rats, the ICG retention rate was below 10% at the 15-minute post-administration; While in the rat with severe hepatocellular carcinoma (HCC), the 15-minute retention rate is over 40% due to poor liver metabolism. With a 785 nm CW laser, the fluorescence dosimeter can evaluate the liver function reserve at a 1/10 clinical dosage of ICG without any blood sampling. In the future, this low-dosage ICG 15-minute retention dosimetry can be applied for the preoperative assessment of hepatectomy or timely perioperative examination.
The development of optical organic nanoparticles (NPs) is desirable and widely studied. However, ... more The development of optical organic nanoparticles (NPs) is desirable and widely studied. However, most organic dyes are water-insoluble such that the derivatization and modification of these dyes are difficult. Herein, we demonstrated a simple platform for the fabrication of organic NPs designed with emissive properties by loading ten different organic dyes (molar masses of 479.1–1081.7 g/mol) into water-soluble polymer nanosponges composed of poly(styrene-alt-maleic acid) (PSMA). The result showed a substantial improvement over the loading of commercial dyes (3.7–50% loading) while preventing their spontaneous aggregation in aqueous solutions. This packaging strategy includes our newly synthesized organic dyes (> 85% loading) designed for OPVs (242), DSSCs (YI-1, YI-3, YI-8), and OLEDs (ADF-1–3, and DTDPTID) applications. These low-cytotoxicity organic NPs exhibited tunable fluorescence from visible to near-infrared (NIR) emission for cellular imaging and biological tracking in v...
The development of optical organic nanoparticles (NPs) is desirable and widely studied. However, ... more The development of optical organic nanoparticles (NPs) is desirable and widely studied. However, most organic chromophores are water-insoluble, and therefore, the derivatization and modification of these chromophores are difficult. Herein, we demonstrated a simple platform for the fabrication of organic nanoemitters by loading ten different organic dyes (molar masses of 479.1-1081.7 g/mol) into water-soluble polymer nanosponges composed of poly(styrene-alt-maleic acid) (PSMA). This nanolization strategy enables the incorporation of small commercial dyes (3.7–50% loading) and newly synthesized large photosensitive molecules (> 85% loading) into PSMA NPs and prevents the spontaneous aggregation of these hydrophobic chromophores in aqueous solutions. These low-cytotoxicity organic nanoparticles exhibited tunable red to near-infrared fluorescence emission for cellular imaging and biological tracking in vivo. Moreover, PSMA NPs loaded with designed NIR-photosensitive molecules were fa...
Immune system plays a critical role in defense against invading microorganisms. Intravital imagin... more Immune system plays a critical role in defense against invading microorganisms. Intravital imaging shows a great potential in monitoring the dynamic process of the immune response. Especially, in vivo optical imaging can track the dynamics of the immune response with high spatiotemporal resolution. Third harmonic generation (THG) can noninvasively reveal cellular morphology, subcellular organelles, and melanin distributions in deep tissues for the application of developmental biology and clinical diagnosis [1]. In deep tissues, this technique has less background interference than reflectance confocal microscopy and better transverse resolution (~500 nm) than infrared-laser based μ-optical coherence tomography (OCT) and spectrally encoded confocal microscopy [2]. We have developed imaging technologies that can detect zebrafish immunocytes in real time using multiphoton confocal microscopy base on a femtosecond laser. By using multiphoton confocal microscopy and Hkz04tTg (CZ61) zebraf...
This study demonstrates the advantage of Dip-Pen Nanolithography (DPN) as a research and design t... more This study demonstrates the advantage of Dip-Pen Nanolithography (DPN) as a research and design tool for metal nano-structure fabrications. We design two different gold nano-structures, which are fabricated by DPN etching method with temperature and humidity control. The plasmon resonance frequencies of both structures are measured with dark field scattering spectroscopy. Our results show that with temperature and humidity control, DPN is highly potential in developing photonic circuit, solar cell and biomedical devices due to the rapid fabrication and cost effectiveness.
A calculation based on surface plasmon coupling condition and Maxwell-Garnett equation was perfor... more A calculation based on surface plasmon coupling condition and Maxwell-Garnett equation was performed for predicting the coupling angle shift and thin film thickness in scanning surface plasmon microscopy (SSPM). The refractive index sensitivity and lateral resolution of an SSPM system was also investigated. The limit of detection of angle shift was 0.01°, the limit of quantification of angle shift was 0.03°, and the sensitivity was around 0.12° shift per nm ZnO film when the film thickness was less than 22.6 nm. Two partially connected Au nano-discs with a center-to-center distance of 1.1 μm could be identified as two peaks. The system was applied to image nanostructure defects and a virus-probe functionalized nanoarray. We expect the potential application in nanobiosensors with further optimization in the future.
The concentration and pH value of the immobilized glucose oxidase (GOD) are critical parameters i... more The concentration and pH value of the immobilized glucose oxidase (GOD) are critical parameters in a glucose sensor. In this study, we develop a glucose fiber sensor integrated with heterodyne interferometry to measure the phase difference arising from the chemical reaction between glucose and GOD. Our studies show that the response time and resolution of this sensor will be strongly affected by the pH and concentration properties of GOD. In addition, the results show good linearity between the calibration curve for the glucose solution and serum based sample. The response time of this sensor can be shorter than 3 seconds and best resolutions are 0.1 and 0.136 mg/dl for glucose solution and serum based sample, respectively.
The concentration and pH value of the immobilized glucose oxidase (GOD) are critical parameters i... more The concentration and pH value of the immobilized glucose oxidase (GOD) are critical parameters in a glucose sensor. In this study, we develop a glucose fiber sensor integrated with heterodyne interferometry to measure the phase difference arising from the chemical reaction between glucose and GOD. Our studies show that the response time and resolution of this sensor will be strongly affected by the pH and concentration properties of GOD. In addition, the results show good linearity between the calibration curve for the glucose solution and serum based sample. The response time of this sensor can be shorter than 3 seconds and best resolutions are 0.1 and 0.136 mg/dl for glucose solution and serum based sample, respectively.
Nanostructures were fabricated using laser irradiating at the conductive tip of an atomic force m... more Nanostructures were fabricated using laser irradiating at the conductive tip of an atomic force microscope (AFM). The conductive tip behaves like an antenna, which receives electromagnetic energy from the laser beam and then emits electromagnetic wave in nanometer range. In this study, a commercial atomic force microscope, combined with a 514.5 nm argon ion laser and a vertically-polarized 532 nm Nd:YAG pulsed laser, was utilized for modification on the gold and 1813 photo-resist films. AFM imaging and nanostructure modification were conducted at the contact-constant-height mode. AFM images were obtained right after the laser irradiations to monitor the modification process. The effect of the laser intensity, the optical field enhancement, and the thermal expansion of the tip under laser irradiation are investigated.Nanostructures were fabricated using laser irradiating at the conductive tip of an atomic force microscope (AFM). The conductive tip behaves like an antenna, which receives electromagnetic energy from the laser beam and then emits electromagnetic wave in nanometer range. In this study, a commercial atomic force microscope, combined with a 514.5 nm argon ion laser and a vertically-polarized 532 nm Nd:YAG pulsed laser, was utilized for modification on the gold and 1813 photo-resist films. AFM imaging and nanostructure modification were conducted at the contact-constant-height mode. AFM images were obtained right after the laser irradiations to monitor the modification process. The effect of the laser intensity, the optical field enhancement, and the thermal expansion of the tip under laser irradiation are investigated.
Opto-Electronics and Communications Conference, Jul 4, 2011
We developed a glucose fiber sensor for measuring the glucose concentration in serum with heterod... more We developed a glucose fiber sensor for measuring the glucose concentration in serum with heterodyne interferometry. The best response time and the resolutions of the fiber sensor are 1.3 seconds and 0.152 mg/dl, respectively. Besides, the fiber sensor can be reused more than 10 times.
The concentration and pH value of the immobilized glucose oxidase (GOD) are critical parameters i... more The concentration and pH value of the immobilized glucose oxidase (GOD) are critical parameters in a glucose sensor. In this study, we develop a glucose fiber sensor integrated with heterodyne interferometry to measure the phase difference arising from the chemical reaction between glucose and GOD. Our studies show that the response time and resolution of this sensor will be strongly affected by the pH and concentration properties of GOD. In addition, the results show good linearity between the calibration curve for the glucose solution and serum based sample. The response time of this sensor can be shorter than 3 seconds and best resolutions are 0.1 and 0.136 mg/dl for glucose solution and serum based sample, respectively.
Using in vivo multiphoton fluorescent dosimetry, we demonstrate that the clearance dynamics of In... more Using in vivo multiphoton fluorescent dosimetry, we demonstrate that the clearance dynamics of Indocyanine Green (ICG) in the blood can quickly reveal liver function reserve. In normal rats, the ICG retention rate was below 10% at the 15-minute post-administration; While in the rat with severe hepatocellular carcinoma (HCC), the 15-minute retention rate is over 40% due to poor liver metabolism. With a 785 nm CW laser, the fluorescence dosimeter can evaluate the liver function reserve at a 1/10 clinical dosage of ICG without any blood sampling. In the future, this low-dosage ICG 15-minute retention dosimetry can be applied for the preoperative assessment of hepatectomy or timely perioperative examination.
The development of optical organic nanoparticles (NPs) is desirable and widely studied. However, ... more The development of optical organic nanoparticles (NPs) is desirable and widely studied. However, most organic dyes are water-insoluble such that the derivatization and modification of these dyes are difficult. Herein, we demonstrated a simple platform for the fabrication of organic NPs designed with emissive properties by loading ten different organic dyes (molar masses of 479.1–1081.7 g/mol) into water-soluble polymer nanosponges composed of poly(styrene-alt-maleic acid) (PSMA). The result showed a substantial improvement over the loading of commercial dyes (3.7–50% loading) while preventing their spontaneous aggregation in aqueous solutions. This packaging strategy includes our newly synthesized organic dyes (> 85% loading) designed for OPVs (242), DSSCs (YI-1, YI-3, YI-8), and OLEDs (ADF-1–3, and DTDPTID) applications. These low-cytotoxicity organic NPs exhibited tunable fluorescence from visible to near-infrared (NIR) emission for cellular imaging and biological tracking in v...
The development of optical organic nanoparticles (NPs) is desirable and widely studied. However, ... more The development of optical organic nanoparticles (NPs) is desirable and widely studied. However, most organic chromophores are water-insoluble, and therefore, the derivatization and modification of these chromophores are difficult. Herein, we demonstrated a simple platform for the fabrication of organic nanoemitters by loading ten different organic dyes (molar masses of 479.1-1081.7 g/mol) into water-soluble polymer nanosponges composed of poly(styrene-alt-maleic acid) (PSMA). This nanolization strategy enables the incorporation of small commercial dyes (3.7–50% loading) and newly synthesized large photosensitive molecules (> 85% loading) into PSMA NPs and prevents the spontaneous aggregation of these hydrophobic chromophores in aqueous solutions. These low-cytotoxicity organic nanoparticles exhibited tunable red to near-infrared fluorescence emission for cellular imaging and biological tracking in vivo. Moreover, PSMA NPs loaded with designed NIR-photosensitive molecules were fa...
Immune system plays a critical role in defense against invading microorganisms. Intravital imagin... more Immune system plays a critical role in defense against invading microorganisms. Intravital imaging shows a great potential in monitoring the dynamic process of the immune response. Especially, in vivo optical imaging can track the dynamics of the immune response with high spatiotemporal resolution. Third harmonic generation (THG) can noninvasively reveal cellular morphology, subcellular organelles, and melanin distributions in deep tissues for the application of developmental biology and clinical diagnosis [1]. In deep tissues, this technique has less background interference than reflectance confocal microscopy and better transverse resolution (~500 nm) than infrared-laser based μ-optical coherence tomography (OCT) and spectrally encoded confocal microscopy [2]. We have developed imaging technologies that can detect zebrafish immunocytes in real time using multiphoton confocal microscopy base on a femtosecond laser. By using multiphoton confocal microscopy and Hkz04tTg (CZ61) zebraf...
This study demonstrates the advantage of Dip-Pen Nanolithography (DPN) as a research and design t... more This study demonstrates the advantage of Dip-Pen Nanolithography (DPN) as a research and design tool for metal nano-structure fabrications. We design two different gold nano-structures, which are fabricated by DPN etching method with temperature and humidity control. The plasmon resonance frequencies of both structures are measured with dark field scattering spectroscopy. Our results show that with temperature and humidity control, DPN is highly potential in developing photonic circuit, solar cell and biomedical devices due to the rapid fabrication and cost effectiveness.
A calculation based on surface plasmon coupling condition and Maxwell-Garnett equation was perfor... more A calculation based on surface plasmon coupling condition and Maxwell-Garnett equation was performed for predicting the coupling angle shift and thin film thickness in scanning surface plasmon microscopy (SSPM). The refractive index sensitivity and lateral resolution of an SSPM system was also investigated. The limit of detection of angle shift was 0.01°, the limit of quantification of angle shift was 0.03°, and the sensitivity was around 0.12° shift per nm ZnO film when the film thickness was less than 22.6 nm. Two partially connected Au nano-discs with a center-to-center distance of 1.1 μm could be identified as two peaks. The system was applied to image nanostructure defects and a virus-probe functionalized nanoarray. We expect the potential application in nanobiosensors with further optimization in the future.
The concentration and pH value of the immobilized glucose oxidase (GOD) are critical parameters i... more The concentration and pH value of the immobilized glucose oxidase (GOD) are critical parameters in a glucose sensor. In this study, we develop a glucose fiber sensor integrated with heterodyne interferometry to measure the phase difference arising from the chemical reaction between glucose and GOD. Our studies show that the response time and resolution of this sensor will be strongly affected by the pH and concentration properties of GOD. In addition, the results show good linearity between the calibration curve for the glucose solution and serum based sample. The response time of this sensor can be shorter than 3 seconds and best resolutions are 0.1 and 0.136 mg/dl for glucose solution and serum based sample, respectively.
The concentration and pH value of the immobilized glucose oxidase (GOD) are critical parameters i... more The concentration and pH value of the immobilized glucose oxidase (GOD) are critical parameters in a glucose sensor. In this study, we develop a glucose fiber sensor integrated with heterodyne interferometry to measure the phase difference arising from the chemical reaction between glucose and GOD. Our studies show that the response time and resolution of this sensor will be strongly affected by the pH and concentration properties of GOD. In addition, the results show good linearity between the calibration curve for the glucose solution and serum based sample. The response time of this sensor can be shorter than 3 seconds and best resolutions are 0.1 and 0.136 mg/dl for glucose solution and serum based sample, respectively.
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Papers by Yin-Lin Lu