The mechanical stimulation from extracellular matrix could regulate physiological behavior ... more The mechanical stimulation from extracellular matrix could regulate physiological behavior of cells through the mechanism of mechanotransduction. Previous researches had shown that apoptosis could be regulated by the size of the cell adhesion area. However, the regulation of cell apoptosis by different adhesion shape with the same area is still unclear. This work focused on the regulation of apoptosis for bone marrow mesenchymal stem cells (MSCs) by different circularity and area of adhesion geometry. We manufactured micro-pattern surface which was suitable for adhesion of MSCs by the technique of micro-contact printing. Three types of geometry for individual island of micro-pattern were designed. We adopted terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) method to detect cell apoptosis. This research shows that the adhesion geometry which has smaller area and greater circularity will promote apoptosis of MSCs. This indicates that MSCs may prefer to live on the surface without any restrict. Our studies focused on the significantly important problem about interaction between extracellular matrix and physiological behavior of mesenchymal stem cells.
Mechanical properties of cells play an important role in their interaction with the extracellular... more Mechanical properties of cells play an important role in their interaction with the extracellular matrix as well as the mechanotransduction process. Several in vitro techniques have been developed to determine the mechanical properties of cells, but none of them can measure the viscoelastic properties of an individual adherent cell in fluid flow non-invasively. In this study, techniques of fluid-structure interaction (FSI) finite element method and quasi-3-dimensional (quasi-3D) cell microscopy were innovatively applied to the frequently used flow chamber experiment, where an adherent cell was subjected to fluid flow. A new non-invasive approach, with cells at close to physiological conditions, was established to determine the viscoelastic properties of individual cells. The results showed an instantaneous modulus of osteocytes of 0.49 7 0.11 kPa, an equilibrium modulus of 0.31 7 0.044 kPa, and an apparent viscosity coefficient of 4.077 1.23 kPa s. This new quantitative approach not only provides an excellent means to measure cell mechanical properties, but also may help to elucidate the mechanotransduction mechanisms for a variety of cells under fluid flow stimulation.
Mechanical properties of cells play an important role in their interaction with the extracellular... more Mechanical properties of cells play an important role in their interaction with the extracellular matrix as well as the mechanotransduction process. Several in vitro techniques have been developed to determine the mechanical properties of cells, but none of them can measure the viscoelastic properties of an individual adherent cell in fluid flow non-invasively. In this study, techniques of fluid-structure interaction (FSI) finite element method and quasi-3-dimensional (quasi-3D) cell microscopy were innovatively applied to the frequently used flow chamber experiment, where an adherent cell was subjected to fluid flow. A new non-invasive approach, with cells at close to physiological conditions, was established to determine the viscoelastic properties of individual cells. The results showed an instantaneous modulus of osteocytes of 0.49 7 0.11 kPa, an equilibrium modulus of 0.31 7 0.044 kPa, and an apparent viscosity coefficient of 4.077 1.23 kPa s. This new quantitative approach not only provides an excellent means to measure cell mechanical properties, but also may help to elucidate the mechanotransduction mechanisms for a variety of cells under fluid flow stimulation.
The mechanical stimulation from extracellular matrix could regulate physiological behavior ... more The mechanical stimulation from extracellular matrix could regulate physiological behavior of cells through the mechanism of mechanotransduction. Previous researches had shown that apoptosis could be regulated by the size of the cell adhesion area. However, the regulation of cell apoptosis by different adhesion shape with the same area is still unclear. This work focused on the regulation of apoptosis for bone marrow mesenchymal stem cells (MSCs) by different circularity and area of adhesion geometry. We manufactured micro-pattern surface which was suitable for adhesion of MSCs by the technique of micro-contact printing. Three types of geometry for individual island of micro-pattern were designed. We adopted terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) method to detect cell apoptosis. This research shows that the adhesion geometry which has smaller area and greater circularity will promote apoptosis of MSCs. This indicates that MSCs may prefer to live on the surface without any restrict. Our studies focused on the significantly important problem about interaction between extracellular matrix and physiological behavior of mesenchymal stem cells.
Mechanical properties of cells play an important role in their interaction with the extracellular... more Mechanical properties of cells play an important role in their interaction with the extracellular matrix as well as the mechanotransduction process. Several in vitro techniques have been developed to determine the mechanical properties of cells, but none of them can measure the viscoelastic properties of an individual adherent cell in fluid flow non-invasively. In this study, techniques of fluid-structure interaction (FSI) finite element method and quasi-3-dimensional (quasi-3D) cell microscopy were innovatively applied to the frequently used flow chamber experiment, where an adherent cell was subjected to fluid flow. A new non-invasive approach, with cells at close to physiological conditions, was established to determine the viscoelastic properties of individual cells. The results showed an instantaneous modulus of osteocytes of 0.49 7 0.11 kPa, an equilibrium modulus of 0.31 7 0.044 kPa, and an apparent viscosity coefficient of 4.077 1.23 kPa s. This new quantitative approach not only provides an excellent means to measure cell mechanical properties, but also may help to elucidate the mechanotransduction mechanisms for a variety of cells under fluid flow stimulation.
Mechanical properties of cells play an important role in their interaction with the extracellular... more Mechanical properties of cells play an important role in their interaction with the extracellular matrix as well as the mechanotransduction process. Several in vitro techniques have been developed to determine the mechanical properties of cells, but none of them can measure the viscoelastic properties of an individual adherent cell in fluid flow non-invasively. In this study, techniques of fluid-structure interaction (FSI) finite element method and quasi-3-dimensional (quasi-3D) cell microscopy were innovatively applied to the frequently used flow chamber experiment, where an adherent cell was subjected to fluid flow. A new non-invasive approach, with cells at close to physiological conditions, was established to determine the viscoelastic properties of individual cells. The results showed an instantaneous modulus of osteocytes of 0.49 7 0.11 kPa, an equilibrium modulus of 0.31 7 0.044 kPa, and an apparent viscosity coefficient of 4.077 1.23 kPa s. This new quantitative approach not only provides an excellent means to measure cell mechanical properties, but also may help to elucidate the mechanotransduction mechanisms for a variety of cells under fluid flow stimulation.
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Papers by jun qiu
apoptosis of MSCs. This indicates that MSCs may prefer to live on the surface without any restrict. Our studies focused on the significantly important problem about interaction between extracellular matrix and physiological behavior of mesenchymal stem cells.
apoptosis of MSCs. This indicates that MSCs may prefer to live on the surface without any restrict. Our studies focused on the significantly important problem about interaction between extracellular matrix and physiological behavior of mesenchymal stem cells.