Papers by Laura Elia Martínez Garza
Toxicon, 1990
GONZÂLEZ ALANIS. Effects of toxin T-544 from the Karwinskia humboldtiana (buckthorn) plant upon m... more GONZÂLEZ ALANIS. Effects of toxin T-544 from the Karwinskia humboldtiana (buckthorn) plant upon mouse embryos explanted at 11 days. Toxicon 28, 449-452, 1990 .-Eleven-day mouse embryos were exposed to the K. humboldtiana toxin T-544 for 24 hr. At the end of the culture period, embryos were examined grossly for malformations and biochemically for altered protein levels. There was a significant difference in malformations in those embryos exposed to 0.05 and 0.2~g/ml of toxin compared with controls. Embryo protein content was significantly lower in those embryos exposed to 0.1~,g/ml of T-544 compared with control group.
Toxicology, Sep 1, 1994
Toxin T-514 is a dimeric anthracenone isolated from the Karwinskia humboldtiana (buckthorn) plant... more Toxin T-514 is a dimeric anthracenone isolated from the Karwinskia humboldtiana (buckthorn) plant. Its potential anti-neoplastic effect was evaluated in vitro and the results obtained were compared with the effect of other known anti-cancer agents. Normal and malignant continuous cell lines were tested. After a 72-h exposure, neoplastic cells derived from hepatic, pulmonary and colonic tissues were more sensitive to toxin T-514 than normal cells from the corresponding organ. Hepatoma cells and colon adenocarcinoma CT50 values were < 10 micrograms/ml. Lung adenocarcinoma, undifferentiated bronchogenic cancer cells and small cell carcinoma CT50 values were < 20 micrograms/ml. All benign cell CT50 levels tested were > 113 micrograms/ml. This in vitro selective toxicity found with toxin T-514 was also seen with 5-fluororacil and mitomycin for colon adenocarcinoma and with epidoxorubicin for undifferentiated bronchogenic cancer cells.
Research Square (Research Square), Jul 6, 2020
Purpose: Folic acid and homocysteine levels are in uenced by the circulating levels of estradiol.... more Purpose: Folic acid and homocysteine levels are in uenced by the circulating levels of estradiol. Little is known about the behavior of these metabolites during an in vitro fertilization cycle, where superovulation protocols induce a major increased in the levels of estradiol and other hormones. Methods: We performed a prospective, comparative, transversal, descriptive and observational study that includes 49 cases (group A), which were patients that entered a fertility program, and 14 controls (group B), which correspond to the donor group. In both groups, the levels of folic acid, estradiol and homocysteine as well as the MTHFR polymorphism were determined. Results: None of the patients has subnormal levels of folic acid or homocysteine and these levels were not modi ed after ovarian stimulation. We observed that homocysteine concentration tended to decrease with elevated estradiol levels produced by the stimulation of the ovary. The presence of allele C677T MTHFR was high in the populations evaluated in this study, with a particular high incidence of the TT allele. Conclusion: The levels of plasma and intracellular folate, and homocysteine do not change during hormonal stimulation.
The journal of experimental zoology, Dec 10, 1982
Mouse embryos at day 9 of development were cultured for 24 to 42 h in 50% fetal calf serum and 50... more Mouse embryos at day 9 of development were cultured for 24 to 42 h in 50% fetal calf serum and 50% Waymouth's medium containing 0.5 pglml insulin supplemented with various amounts of progesterone and estradiol-17-& Unmodified medium contained approximately 0.2% of the normal maternal blood levels for that stage of pregnancy. The addition of 1 x 10-7M progesterone to the medium brought the level near that of the normal maternal circulating amount and appeared to be beneficial for in vitro development. After 24 h of cultivation there was a statistically significant increase in somite number, the number of embryos developing posterior limb buds, and protein accumulation over the control embryos. The addition of small amounts of estradiol-17P (1 x 10-l0M) increased the protein accumulation of the embryo over that of progesterone alone and seems to enhance the beneficial effects of progesterone addition.
The Journal of Clinical Endocrinology and Metabolism, Sep 1, 2010
Context: Genetic lipodystrophies are rare disorders characterized by partial or complete loss of ... more Context: Genetic lipodystrophies are rare disorders characterized by partial or complete loss of adipose tissue and predisposition to insulin resistance and its complications such as diabetes mellitus, hypertriglyceridemia, hepatic steatosis, acanthosis nigricans, and polycystic ovarian syndrome. Objective: The objective of the study was to report a novel autosomal recessive lipodystrophy syndrome. Results: We report the detailed phenotype of two males and one female patient, 26-34 yr old, belonging to two pedigrees with an autosomal recessive syndrome presenting with childhoodonset lipodystrophy, muscle atrophy, severe joint contractures, erythematous skin lesions, and microcytic anemia. Other variable clinical features include hypergammaglobulinemia, hepatosplenomegaly, generalized seizures, and basal ganglia calcification. None of the patients had diabetes mellitus or acanthosis nigricans. Two had mild hypertriglyceridemia and all had low levels of high-density lipoprotein cholesterol. Skin biopsy of an erythematous nodular skin lesion from one of the patients revealed evidence of panniculitis. The lipodystrophy initially affected the upper body but later became generalized involving abdomen and lower extremities as well. Conclusions: We conclude that these patients represent a novel autoinflammatory syndrome resulting in joint contractures, muscle atrophy, microcytic anemia, and panniculitis-induced lipodystrophy. The molecular genetic basis of this disorder remains to be elucidated. (J Clin Endocrinol Metab 95: E58-E63, 2010) Patients and Methods All patients, their family members, and the normal healthy volunteer gave written informed consent. The study protocol was
The journal of experimental zoology, Oct 10, 1982
A method for cultivation of 11-day mouse embryos (plug day = day 0) is presented. Competence of t... more A method for cultivation of 11-day mouse embryos (plug day = day 0) is presented. Competence of the visceral yolk sac and serum dilutions were tested. Eleven day mouse embryos were cultivated for 24 hours with the visceral yolk sac either opened or left intact. Fetal calf serum (FCS) at 0%, 25%, and 50% dilutions balanced with Waymouths medium was used as cultivation medium. Viability and growth of the embryos were evaluated by heart rate at 6 hours, and protein accumulation and differentiation at 24 hours. At 6 hours, best results were found in those embryos with the visceral yolk sac intact. Serum dilutions at this time were not important for viability. At 24 hours, only those embryos cultivated with the visceral yolk sac opened were alive. Significant increase in viability was obtained when a 50% dilution of FCS was used. There was a significant increase in protein accumulation for viable embryos at 24 hours.
Trabajo presentado en la 9a Reunion Internacional de Investigadores en Productos Naturales, celeb... more Trabajo presentado en la 9a Reunion Internacional de Investigadores en Productos Naturales, celebrada en Pachuca (Mexico) del 29 al 31 Mayo de 2013
Journal of Clinical Oncology, 2012
e15525 Background: Human papillomavirus (HPV) is associated with carcinoma of the cervix (CC). Th... more e15525 Background: Human papillomavirus (HPV) is associated with carcinoma of the cervix (CC). There is evidence that clearance of the virus occurs after chemoradiotherapy which is the standard treatment for this disease. Methods: Eligible patients with bulky IB to IVA CC were treated with once weekly cisplatin (40 mg/m2) chemotherapy and standard pelvic teletherapy and brachyterapy. HPV detection and typing was performed with DNA microarrays from liquid based cytologies before and 4 to 6 weeks after treatment completion. Clinical response was assesed by physical exam and liquid based cytology. Results: 23 patients (pt) with CC , were tested for HPV. Their age ranged from 33 to 80 years (mean 65), clinical stage I 8.6%, StageII 39%, stageIII 25.9% and stageIV 26.5%.VPH was found in 20 patients (87%). 34% of the patients had more than one type of HPV. 13 types of VPH were found,the most prevalent type was 16 in 47.8%, followed by type 72 in 13%.7 patients were lost to follow up durin...
Medicina Universitaria, 2001
Southwestern Entomologist, 2014
Abstract. When a corpse is found, typical questions of medico-criminal entomology involve: death ... more Abstract. When a corpse is found, typical questions of medico-criminal entomology involve: death time estimation or post-mortem interval determination and less frequently, the causes and place where death occurred. Entomological succession represents a powerful tool to calculate or deduce these parameters or questions. Sequences of cytochrome oxidase subunit I (COI) gene from nine species of Diptera of forensic importance registered in GenBank were used in this study. Sequences were aligned and a consensus sequence deduced for each species. A pair of oligonucleotides was designed in high conserve regions that allowed theoretical and simultaneous the co-amplification of the nine species and within this segment we used restriction enzymes (Eco57MI and BscAI) that generate different electrophoretic patterns for each species. Tentatively, with the same pair of oligonucleotides, it is possible to amplify a segment of the COI gene of nine insects of forensic importance and using a simple combination of restriction enzymes, be able to identify them. It is a technique that can be used in any laboratory with basic equipment and is an economically affordable alternative.
Teratology, 2002
BackgroundNuevo León is a state in northeastern Mexico, near the border of Texas. Mean mortality ... more BackgroundNuevo León is a state in northeastern Mexico, near the border of Texas. Mean mortality rate from 1996–98 due to anencephaly cases was 0.6/1,000. In 1999 a surveillance program for the registry and prevention of neural tube defects (NTD) cases was initiated.MethodsCases were obtained from hospitals and OB‐GYN clinics by immediate notification, death certificates, or fetal death registries. Only isolated cases of NTD were included. In August 1999 a folic acid campaign was initiated with the free distribution of the vitamin to low‐income women with a recommendation to take a 5.0‐mg pill once a week. Number of cases and rates from 1999 to 2001 were compared (χ2 test).ResultsAfter 2 years there has been a significant reduction in the number of cases and rates. In 1999 there were 95 NTD cases and in the years 2000 and 2001 there were only 59 and 55 respectively (P < 0.001). NTD rate decreased from 1.04/1,000 in 1999 to 0.58/1,000 in 2001. Anencephaly and spina bifida rates de...
[22nd International Nursing Research Congress-Research Presentation] Purpose: The purpose of this... more [22nd International Nursing Research Congress-Research Presentation] Purpose: The purpose of this study was to describe the presence of obesity (Ob) in family members participating in a research study in a metropolitan area in North East Mexico. In addition, ...
This dataset combines pedigree, genotypes, clinical and biochemical data of 37 families of Northe... more This dataset combines pedigree, genotypes, clinical and biochemical data of 37 families of Northeastern Mexico. Primary reference is the article: Gallardo‑Blanco, H.L., Villarreal‑Perez, J.Z., Cerda‑Flores, R.M., Figueroa, A., Sanchez‑Dominguez, C.N., Gutierrez‑Valverde, J.M. ... Martinez‑Garza, L.E. (2017). Genetic variants in KCNJ11, TCF7L2 and HNF4A are associated with type 2 diabetes, BMI and dyslipidemia in families of Northeastern Mexico: A pilot study. Experimental and Therapeutic Medicine, 13, 523-529. https://doi.org/10.3892/etm.2016.3990 <strong>If you use these data please cite the corresponding manuscript, which can be downloaded here:</strong> https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5348709/ https://www.spandidos-publications.com/10.3892/etm.2016.3990 This dataset contains genotypes for the following SNPs: rs2986742 rs4846051 rs1801131 rs1801133 rs6541030 rs12130799 rs11208654 rs1137100 rs12405556 rs3118378 rs3737576 rs10923931 rs7554936 rs3737787 rs251...
International Journal of Medical Students
Background: Metabolic syndrome (MS), a combination of diabetes, high blood pressure and obesity, ... more Background: Metabolic syndrome (MS), a combination of diabetes, high blood pressure and obesity, is a well-known risk factor for developing non-alcoholic fatty liver disease, condition that can lead to serious liver damage such as liver fibrosis (LF), which is characterized by excessive deposition of connective tissue, progressing to cirrhosis and hepatocellular carcinoma. Nevertheless, subjects without MS may also develop LF. Non-invasive LF predictors based upon anthropometric and biochemical data have been reported. Aim: To compare anthropometric, genetic, and biochemical parameters in subjects with or without MS, and at risk for developing liver fibrosis. Methods: A randomized sample of 200 individuals was taken from the 2015 Nuevo León State Health Survey. Inclusion criteria were age ≥18 and a previously stored blood sample. According to the parameters obtained, subjects were classified as either with or without MS and their NAFLD fibrosis score was calculated considering varia...
American Journal of Perinatology
Background: Glucose-6-phosphate dehydrogenase deficiency (G6PDd) is the most common enzymatic dis... more Background: Glucose-6-phosphate dehydrogenase deficiency (G6PDd) is the most common enzymatic disease worldwide and the prevalence is not well established because of the lack of screening. Objective: This study aimed to estimate the prevalence of G6PDd in a Hispanic population from Northeast Mexico. Study Design: In this retrospective study, a database was used to analyze the G6PDd in neonates included in the expanded newborn metabolic screening of inherited metabolic disorders during a period of four years through the GSP Neonatal G6 kit (PerkinElmer). Results: Among 96,152 (48,462 male) neonates screened for G6PD enzyme activity, a total of 566 (0.58%) cases were deficient for G6PD. Of those 566 patients, 469 (82.8%) attended the second test and the other 97 (17.2%) patients were lost. Of those 469 who did attend, 384 (81.9%) neonates were deficient in the second test and 85 (18.1%) were normal. With the data collected, 384 neonates were confirmed with G6PDd, 348 (88.6%) were male...
Innovations in clinical neuroscience, Aug 13, 2022
The paper investigates the survival of two South-Guan minority dialects, Leteh and Efutu, in the ... more The paper investigates the survival of two South-Guan minority dialects, Leteh and Efutu, in the context of the Ghana language-in-education policy. The study is done from the perspective of the UNESCO Universal Declaration on Linguistic Rights (1996). In every multilingual state, the formulation of policies concerning language use has always presented challenges. The government has to decide which of the languages need to be promoted and for what purposes. In Ghana, since the introduction of formal education, English has indubitably been the language of education, trade, law, media, government and administration. However, there has always been a debate surrounding the language-in-education policy, especially at the basic level of education. The argument has always been whether English should be emphasised or Ghanaian languages. For purposes of formal education, the government of Ghana has promoted nine languages known as governmentsponsored languages. These are languages which have literary tradition and can be used as media of instruction in schools. This decision was to the detriment of some Ghanaian languages; languages which are often described as minority languages, and which are not governmentsponsored. The paper argues that, if language and culture are intertwined, and the culture of a people must be preserved, then language policymakers need to consider the linguistic rights of speakers of the so-called minority languages. Data for the study were sourced from language surveys and observation.
The prevalence of type 2 diabetes (T2D) is rapidly increasing worldwide. In Mexico, the prevalenc... more The prevalence of type 2 diabetes (T2D) is rapidly increasing worldwide. In Mexico, the prevalence of T2D is 14.4%, with early-onset T2D representing 21.5% of all cases of diabetes. 1 This represents a sig-nificant public health burden due to disability and pre-mature death. The risk of developing T2D is determined by ge-netic and environmental factors. 2 The aim of this study was to identify susceptibility loci related to early onset T2D in our population. Material and Methods We studied 128 SNPs in or near 42 genes, most of which had been replicated in other populations. A case-control association study comprising 186 controls, 211 early-onset T2D (diagnosed before age 45 years) and 173 late-onset T2D (diagnosed at age 45 years or older) individuals was conducted. We consider as significant SNP association if it had a p value < 0.001. Results Subjects with T2D had higher mass body index than controls (27.5 vs 25.7 kg/m2, P <0.0001). The median age for early-onset T2D, late-o...
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Papers by Laura Elia Martínez Garza