Papers by Nelson Colihueque
Two rainbow trout female populations (Wt-01 y Wt-02) were studied, which a percentage of individu... more Two rainbow trout female populations (Wt-01 y Wt-02) were studied, which a percentage of individuals displayed a double annually reproductive cycle (DARC). This trait is characterized by an autumn spawning (normal reproductive cycle) and an additional one at spring (additional reproductive cycle). During seasons 2004 and 2005, the specimens that registered an additional spawning besides the normal, included 14.4% (Wt-01) and 36.2% (Wt-02) of the population. These females belong to double-cycler specimens, while those who spawn once a year are mono-cyclers. In both populations, the reproductive performance of normal reproductive cycle was comparatively evaluated in relation to the additional reproductive cycle, using seven biometricreproductive variables. The average weight of the spawning females (Wt-01:
Figure 2 from: Colihueque N, Cabello J, Fuentes-Moliz A (2022) Genetic divergence and demography of pudu deer (Pudu puda) in five provinces of southern Chile, analyzed through latitudinal and longitudinal ranges. Neotropical Biology and Conservation 17(2): 117-142. https://doi.org/10.3897/neotro...
Supplementary material 2 from: Colihueque N, Gantz A, Parraguez M (2021) Revealing the biodiversity of Chilean birds through the COI barcode approach. ZooKeys 1016: 143-161. https://doi.org/10.3897/zookeys.1016.51866
Figure 2 from: Colihueque N, Gantz A, Parraguez M (2021) Revealing the biodiversity of Chilean birds through the COI barcode approach. ZooKeys 1016: 143-161. https://doi.org/10.3897/zookeys.1016.51866
Figure 3 from: Colihueque N, Gantz A, Parraguez M (2021) Revealing the biodiversity of Chilean birds through the COI barcode approach. ZooKeys 1016: 143-161. https://doi.org/10.3897/zookeys.1016.51866
The cientificWorldJOURNAL Research Article Comparative Study of Various E. coli Strains for Biohydrogen Production Applying Response Surface Methodology
Copyright © 2012 Péter Bakonyi et al. This is an open access article distributed under the Creat... more Copyright © 2012 Péter Bakonyi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The proper strategy to establish efficient hydrogen-producing biosystems is the biochemical, physiological characterization of hydrogen-producing microbes followed by metabolic engineering in order to give extraordinary properties to the strains and, finally, bioprocess optimization to realize enhanced hydrogen fermentation capability. In present paper, it was aimed to show the utility both of strain engineering and process optimization through a comparative study of wild-type and genetically modified E. coli strains, where the effect of two major operational factors (substrate concentration and pH) on bioH2 production was investigated by experimental design and response surface methodology (RSM) was used to determine the suitable co...
Supplementary material 1 from: Colihueque N, Gantz A, Parraguez M (2021) Revealing the biodiversity of Chilean birds through the COI barcode approach. ZooKeys 1016: 143-161. https://doi.org/10.3897/zookeys.1016.51866
Reproductive performance assessed during three spawning seasons in a naturalized rainbow trout population from southern Chile
Fisheries Research, 2021
Abstract Naturalized rainbow trout (Oncorhynchus mykiss) populations are an important recreationa... more Abstract Naturalized rainbow trout (Oncorhynchus mykiss) populations are an important recreational fishing resource in the Araucanian lakes of southern Chile, but few reproductive studies have been undertaken to assess their sustainability. It is important to address this issue, since this analysis may provide information for improving management activities. In this study we carried out a biometric and reproductive analysis of naturalized rainbow trout breeders to describe their performance and to explore the variables that could determine fecundity, fertilization in addition to egg survival and size. We collected naturalized rainbow trout breeders from six tributaries of Calafquen Lake, during 2006, 2007 and 2012 to record their biometric and reproductive traits. Spawning and incubation were performed under controlled conditions in a hatchery facility to improve the recording process. In each spawning season, total fecundity (TF, No. of eggs/female), relative fecundity (RF, No. of eggs/kg female), fertilization rate (FR, %), egg diameter (ED, mm) and eyed egg survival (EES, %) were modeled as a function of biometric and reproductive parameters, using general linear mixed models. We used this statistical tool to explore the relationships between these reproductive traits and other breeder traits in order to identify all the potential combinations that could be linked to TF, RF, FR, ED and EES. The spawning period ranged from September to November (i.e., spring spawning season), with a clear spawning peak in October in all reproductive seasons. Mean TF (from 2275.9 to 3438.7) and RF (from 1730.5 to 1909.8) fell within the range of, or were close to, data reported for other Araucanian lakes, respectively. Mean values of FR and EES were relatively high, ranging from 88.8% to 93.3% and between 83.4% and 88.8%, respectively. Models indicated that TF is significantly affected by the interaction between female body weight and egg weight, while RF is affected by the combination of female body length and egg weight. In addition, a positive correlation was observed between female body weight and total fecundity, while an inverse association was recorded between relative fecundity and female body length. This trend is in accordance with the trade-off pattern between egg number and fish size described for naturalized populations of rainbow trout from the Northern Hemisphere. However, regression equations output suggests that the reproductive performance of the Calafquen Lake population has a more depleted fecundity in comparison either with naturalized populations from the Northern Hemisphere, or with cultured stocks of this species. Models for ED revealed that this variable is affected by egg weight and also by body length or body weight, all with positive correlations. This result concurs with observations reported for other naturalized populations of rainbow trout from North America, further supporting evidence that larger females of this species produce larger eggs than smaller females. Models for EES revealed the significant positive effect of fertilization rate and relative fecundity, being the latter an unexpected result, given that insignificant correlations between these variables have been found in cultured stocks of this species. Overall, the population analyzed exhibited a regular spawning period, and an optimal reproductive performance in terms of egg survival. To promote the sustainability of the naturalized rainbow trout of Calafquen Lake, we recommend the use of hatchery propagation of wild adults to support the production of fry to be used in subsequent restocking activities. This management effort can benefit because the broodstocks have a short spawning period during their spring run.
The biodiversity of Chilean avifauna has been shaped by geological events, geographical factors a... more The biodiversity of Chilean avifauna has been shaped by geological events, geographical factors and extreme climatic regimens. Molecular genetic studies developed to date have begun to provide valuable information to elucidate the effect of these factors on the evolutionary history of Chilean birds. We conducted a search in two bibliographic databases (PubMed and Scopus) over the period between 1998 and 2018 to identify the number and class of articles published in this research field. The quantification of specific research categories was also performed to assess progress in different molecular genetic issues of Chilean birds. Representative studies of the more active research areas: population structure, phylogeography, systematics and taxonomy and genetic diversity, were briefly commented on. Studies on genetic divergence analysis of cryptic species and the underlying genetics at molecular level of adaptive phenotypes in populations, were very scarce. Several types of mitochondrial and nuclear markers have been used, with COI plus D-loop and microsatellite loci, respectively, being the most widely-used markers. However, high-throughput analyses based on complete mitochondrial genomes have also been published. More than one hundred species have been analyzed; the most studied groups being penguins, followed by New World swallows. Although, to date, molecular genetic studies of Chilean birds still require further analysis, the studies available on some species or groups have provided important data to improve our understanding of the origin and evolution of this avifauna.
The biodiversity of Chilean avifauna has been shaped by geological events, geographical factors a... more The biodiversity of Chilean avifauna has been shaped by geological events, geographical factors and extreme climatic regimens. Molecular genetic studies developed to date have begun to provide valuable information to elucidate the effect of these factors on the evolutionary history of Chilean birds. We conducted a search in two bibliographic databases (PubMed and Scopus) over the period between 1998 and 2018 to identify the number and class of articles published in this research field. The quantification of specific research categories was also performed to assess progress in different molecular genetic issues of Chilean birds. Representative studies of the more active research areas: population structure, phylogeography, systematics and taxonomy and genetic diversity, were briefly commented on. Studies on genetic divergence analysis of cryptic species and the underlying genetics at molecular level of adaptive phenotypes in populations, were very scarce. Several types of mitochondrial and nuclear markers have been used, with COI plus D-loop and microsatellite loci, respectively, being the most widely-used markers. However, high-throughput analyses based on complete mitochondrial genomes have also been published. More than one hundred species have been analyzed; the most studied groups being penguins, followed by New World swallows. Although, to date, molecular genetic studies of Chilean birds still require further analysis, the studies available on some species or groups have provided important data to improve our understanding of the origin and evolution of this avifauna.
Frontiers in Genetics, 2014
Appearance traits in fish, those external body characteristics that influence consumer acceptance... more Appearance traits in fish, those external body characteristics that influence consumer acceptance at point of sale, have come to the forefront of commercial fish farming, as culture profitability is closely linked to management of these traits. Appearance traits comprise mainly body shape and skin pigmentation. Analysis of the genetic basis of these traits in different fish reveals significant genetic variation within populations, indicating potential for their genetic improvement. Work into ascertaining the minor or major genes underlying appearance traits for commercial fish is emerging, with substantial progress in model fish in terms of identifying genes that control body shape and skin colors. In this review, we describe research progress to date, especially with regard to commercial fish, and discuss genomic findings in model fish in order to better address the genetic basis of the traits. Given that appearance traits are important in commercial fish, the genomic information related to this issue promises to accelerate the selection process in coming years.
Scientific World Journal, 2012
This study compares the gonadosomatic index (GSI), oocyte growth (OG), gonadal histology, and pla... more This study compares the gonadosomatic index (GSI), oocyte growth (OG), gonadal histology, and plasma level concentrations of sex hormones (estradiol-17β (E2) and vitellogenin (V)) of twice-spawning (T-SP) and once-spawning (O-SP) females of rainbow trout throughout the additional and the normal reproductive cycle, respectively. In T-SP, the GSI values rapidly increase from May to November, in contrast to O-SP, which showed low and constant GSI values (1.19 to 14.5 and 1.19 to 0.63, resp.). T-SP exhibited a marked increase of OG in the same period, reaching a maximum diameter of 4,900 ± 141.42 μm, in contrast to O-SP, which presented a slow OG. The gonadal histology of T-SP agreed with the general pattern of ovogenesis observed for O-SP (vitellogenesis, ovulation, and recrudescence); however, this process was nonsynchronous between the two breeder groups. Plasma steroid levels showed significant variation during oogenesis, which agreed with the GSI, OG, and gonadal histology patterns. The level of E2 increased to a maximum value of 26.2 ng/mL and 36.0 ng/mL in O-SP and T-SP, respectively, one or two months before the spawning event where vitellogenesis was fully active. The V concentrations followed a pattern similar to those of E2.
Genetics and Molecular Biology, 2010
The rainbow trout is a salmonid fish that occasionally exhibits broodstocks with biannual spawnin... more The rainbow trout is a salmonid fish that occasionally exhibits broodstocks with biannual spawning behavior, a phenomenon known as a double annual reproductive cycle (DARC). Spawning time quantitative trait loci (SPT-QTLs) affect the time of the year that female rainbow trout spawn and may influence expression of the DARC trait. In this study, microsatellite markers linked and unlinked to SPT-QTLs were genotyped to investigate the underlying genetics of this trait. SPT-QTLs influenced the DARC trait since in two case-control comparisons three linked markers (OmyFGT12TUF, One3ASC and One19ASC) had significant levels of allelic frequency differentiation and markercharacter association. Furthermore, alleles of One3ASC and One19ASC had significantly higher frequencies in populations that carried the DARC trait.
Biological Research, 2006
One of the fungal pathogens that causes more agriculture damage is Botrytis cinerea. Botrytis is ... more One of the fungal pathogens that causes more agriculture damage is Botrytis cinerea. Botrytis is a constant threat to crops because the fungus infects a wide range of host species, both native and cultivated. Furthermore, Botrytis persists on plant debris in and on the soil. Some of the most serious diseases caused by Botrytis include gray mold on vegetables and fruits, such as grapes and strawberries. Botrytis also causes secondary soft rot of fruits and vegetables during storage, transit and at the market. In many plant-pathogen interactions, resistance often is associated with the deposition of callose, accumulation of autofluorescent compounds, the synthesis and accumulation of salicylic acid as well as pathogenesis-related proteins. Arabidopsis thaliana has been used as a plant model to study plant-pathogen interaction. The genome of Arabidopsis has been completely sequenced and this plant serves as a good genetic and molecular model. In this study, we demonstrate that Chilean field isolates infect Arabidopsis thaliana and that Arabidopsis subsequently activates several defense response mechanisms associated with a hypersensitive response. Furthermore, we propose that Arabidopsis may be used as a model host species to analyze the diversity associated with infectivity among populations of Botrytis cinerea field isolates.
Physiological factors affecting triploid production in rainbow trout, Oncorhynchus mykiss
Aquaculture, 1993
The influence of three physiological factors related to triploid production was examined for rain... more The influence of three physiological factors related to triploid production was examined for rainbow trout. Individual spawnings of 46 females were used to perform triploid production experiments from 1989 to 1991, with various water temperatures (613.8°C) at stripping and egg ...
Aquaculture, 1992
Twenty protein-coding loci in nine populations of rainbow trout, from different localities in Sou... more Twenty protein-coding loci in nine populations of rainbow trout, from different localities in South Africa, were analyzed by starch-gel and polyacrylamide-gel electrophoresis to determine the degree of genetic diversity present Heterozygosity levels also were determined and ranged between 0.0922 and 0.1208. The relatively high heterozygosity levels are due to the historical mixing of strains and the differences in heterozygosity levels indicate that the populations originated from different natural populations, each with different genetic characteristics. Differences in average heterozygosities suggest that selection based on genotype may form the basis to establish various strains suitable for South African conditions. Selection for economically important traits also is of international importance since trout ova from South Africa are exported to other countries. Genotypic differences seemed to relate to differences in phenotypic characters, growth and survivaL
Aquaculture, 2001
consistent with the Robertsonian-type polymorphism described for rainbow trout. The presence of d... more consistent with the Robertsonian-type polymorphism described for rainbow trout. The presence of different chromosome numbers among strains, could reflect the chromosome differences existing within ancestral trout populations, or the presence of a certain degree of mixing with other populations of different geographical origin. The discovery of specimens with an unexpected sex chromosome is suggestive of an incomplete differentiation of the sex chromosome pair in the rainbow trout.
Neotropical Biology and Conservation
Pudu deer (Pudu puda) is endemic to the temperate rainforests of Chile. Genetic studies at differ... more Pudu deer (Pudu puda) is endemic to the temperate rainforests of Chile. Genetic studies at different geographic scales for this species are required to better determine the genetic divergence within and among populations and their demography across the distribution range. These data can provide unique insights into the species or population status for conservation plans and decision-makers. We analyzed the mtDNA control region (CR) and cytochrome b (Cyt b) sequences of pudu deer in five provinces of southern Chile located at different latitudinal locations (Cautín, Valdivia, Osorno, Llanquihue and Chiloé Island) and three geographic areas within the studied provinces, representative of different longitudinal sites (Andes range, Central Valley and Coastal Range), to understand their genetic divergence and demography. The haplotype (H) and nucleotide (Π) diversities of CR and Cyt b ranged from 0.64286 to 0.98333 and from 0.00575 to 0.01022, respectively. CR diversity was significantly...
Short Communication
www.sbg.org.br Analysis of the association between spawning time QTL markers and the biannual spa... more www.sbg.org.br Analysis of the association between spawning time QTL markers and the biannual spawning behavior in rainbow trout (Oncorhynchus mykiss)
Two rainbow trout female populations (Wt-01 y Wt-02) were studied, which a percentage of individu... more Two rainbow trout female populations (Wt-01 y Wt-02) were studied, which a percentage of individuals displayed a double annually reproductive cycle (DARC). This trait is characterized by an autumn spawning (normal reproductive cycle) and an additional one at spring (additional reproductive cycle). During seasons 2004 and 2005, the specimens that registered an additional spawning besides the normal, included 14.4% (Wt-01) and 36.2% (Wt-02) of the population. These females belong to double-cycler specimens, while those who spawn once a year are mono-cyclers. In both populations, the reproductive performance of normal reproductive cycle was comparatively evaluated in relation to the additional reproductive cycle, using seven biometricreproductive variables. The average weight of the spawning females (Wt-01: 1139 ± 116 g vs 1528 ± 105 g; Wt-02: 1195 ± 217 vs 1512 ± 176), the total fecundity (Wt-01: 2.310 ± 214 vs 3388 ± 299; Wt-02: 2.691 ± 600 vs 3.680 ± 509) and the relative fecundity ...
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Papers by Nelson Colihueque