McVetty, P. B. E., Duncan, R. W., Fernando, W. G. D., Li, G. and Zelmer, C. D. 2012. Red River 18... more McVetty, P. B. E., Duncan, R. W., Fernando, W. G. D., Li, G. and Zelmer, C. D. 2012. Red River 1861 Roundup Ready™ high erucic acid, low glucosinolate summer rape. Can. J. Plant Sci. 92: 1407–1409. Red River 1861 summer rape (Brassica napus L.) is the world's fourth Roundup Ready™ high erucic acid, low glucosinolate cultivar. On average, Red River 1861 yielded 19% more seed, 7 g kg−1 more seed oil and 7 g kg −1 more meal protein than MillenniUM 03 high erucic acid, low glucosinolate summer rape. Red River 1861 has an erucic acid content of 52.6% in isolated field trials of HEAR lines and is adapted to the southern B. napus growing regions of western Canada.
Background: Sequence related amplified polymorphism (SRAP) is commonly used to construct high den... more Background: Sequence related amplified polymorphism (SRAP) is commonly used to construct high density genetic maps, map genes and QTL of important agronomic traits in crops and perform genetic diversity analysis without knowing sequence information. To combine next generation sequencing technology with SRAP, Illumina’s Solexa sequencing was used to sequence tagged SRAP PCR products. Results: Three sets of SRAP primers and three sets of tagging primers were used in 77,568 SRAP PCR reactions and the same number of tagging PCR reactions respectively to produce a pooled sample for Illumina’s Solexa sequencing. After sequencing, 1.28 GB of sequence with over 13 million paired-end sequences was obtained and used to match Solexa sequences with their corresponding SRAP markers and to integrate Solexa sequences on an ultradense genetic map. The ultradense genetic bin map with 465 bins was constructed using a recombinant inbred (RI) line mapping population in B. rapa. For this ultradense gene...
The effect of changing the oxygen concentration from 21 to 2% on photosynthesis of wheat, sunflow... more The effect of changing the oxygen concentration from 21 to 2% on photosynthesis of wheat, sunflower, and soybean was investigated. At low CO2 concentrations and low light intensities, a stimulation of photosynthesis was observed in 2% oxygen compared with the rate in 21% O2. At high CO2 concentrations and high light intensities, a temporary inhibition of photosynthesis was observed when the oxygen concentration was changed from 21 to 2%. In wheat and sunflower, this inhibition was observed at progressively lower CO2 concentrations as temperatures were decreased. In soybean only, a slight inhibition of photosynthesis was observed at higher temperatures. In some cases, especially in plants grown under a low light intensity, a long-lasting (> 45 min) inhibition of photosynthesis was observed. In most cases, however, the inhibition lasted only for several minutes and final rates of photosynthesis in 2% O2 were equal to or greater than the rate of photosynthesis in 21% O2. The stimula...
Clubroot disease is devastating to Brassica crop production when susceptible cultivars are plante... more Clubroot disease is devastating to Brassica crop production when susceptible cultivars are planted in infected fields. European turnips are the most resistant sources and their resistance genes have been introduced into other crops such oilseed rape (Brassica napus L.), Chinese cabbage and other Brassica vegetables. The European clubroot differential (ECD) set contains four turnip accessions (ECD1-4). These ECD turnips exhibited high levels of resistance to clubroot when they were tested under controlled environmental conditions with Canadian field isolates. Gene mapping of the clubroot resistance genes in ECD1-4 were performed and three independent dominant resistance loci were identified. Two resistance loci were mapped on chromosome A03 and the third on chromosome A08. Each ECD turnip accession contained two of these three resistance loci. Some resistance loci were homozygous in ECD accessions while others showed heterozygosity based on the segregation of clubroot resistance in 20 BC 1 families derived from ECD1 to 4. Molecular markers were developed linked to each clubroot resistance loci for the resistance gene introgression in different germplasm.
Evolve is the world’s first ogu INRA CMS hybrid summer rape (Brassica napus L.) Roundup Ready® hi... more Evolve is the world’s first ogu INRA CMS hybrid summer rape (Brassica napus L.) Roundup Ready® high erucic acid, low glucosinolate cultivar. On average, Evolve yielded 12.4 % more seed, 6 g kg-1 more seed oil and 5 g kg -1 less meal protein than Red River 1861, an open-pollinated Roundup Ready® high erucic acid, low glucosinolate summer rape. Evolve has an erucic acid content of 52.3 % in isolated field trials of HEAR genotypes and is adapted to the southern B. napus growing regions of western Canada.
Journal of the American Society for Horticultural Science
There are various clubroot pathogen (Plasmodiophora brassicae) resistance genes within Brassica s... more There are various clubroot pathogen (Plasmodiophora brassicae) resistance genes within Brassica species with european turnip (B. rapa ssp. rapifera) being identified as potentially the best source of resistance for the development of clubroot-resistant cultivars in chinese cabbage (B. rapa ssp. pekinensis). To use clubroot resistance genes effectively, it is necessary to map these genes so that molecular markers inside or closely linked to these resistance genes can be developed. Using molecular marker-assisted selection, the clubroot resistance genes can be effectively transferred from cultivar to cultivar and from species to species. In this report, one clubroot resistance locus was mapped on linkage group A3 using five segregating populations developed from five chinese cabbage cultivars, suggesting that all the five cultivars shared the same clubroot resistance locus. Furthermore, one of these five chinese cabbage cultivars was used to develop a large segregating population to f...
HYHEAR 3 summer rape (Brassica napus L.) is a Roundup Ready®, high erucic acid, low glucosinolate... more HYHEAR 3 summer rape (Brassica napus L.) is a Roundup Ready®, high erucic acid, low glucosinolate cultivar. On average, HYHEAR 3 yielded 15.6% more seed, 4 g kg −1 less seed oil, and 22 g kg −1 less meal protein than Red River 1861 Roundup Ready®, high erucic acid, low glucosinolate summer rape. HYHEAR 3 has an erucic acid content of 50.9% in isolated field trials of high erucic acid rapeseed (HEAR) genotypes and is adapted to the southern B. napus growing regions of western Canada.
The growth of all grass-clump dwarfs is sensitive to temperature with low temperature giving rise... more The growth of all grass-clump dwarfs is sensitive to temperature with low temperature giving rise to the grass-clump phenotype and high temperature producing normal phenotype. A continuous temperature of 26°C is required for normal growth of Type 1 dwarfs, a continuous temperature of 21 °C is required for normal growth of Type 2 dwarfs and a continuous temperature of 16°C is required for normal growth of Type 3 dwarfs. Genetic studies show that the inheritance of the grass-clump characteristic is due to three complementary dominant genes. The grass-clump growth habit is produced as a result of the temperature sensitivity of the apical meristem. In grass-clump plants low temperature treatment results in the cessation of cell division, DNA synthesis and phospholipid synthesis in the apical meristem. The primary temperature lesion has not been identified. Prolonged low temperature treatment of grass-clump plants results in extensive cell necrosis in a region just below the apical meristem; this cell death results in the permanent inactivation of the apical meristem.
Technological Innovations in Major World Oil Crops, Volume 2, 2011
... Applications in Oilseed Rape and Linseed Peter BE McVetty and Rachael Scarth ... Since Libert... more ... Applications in Oilseed Rape and Linseed Peter BE McVetty and Rachael Scarth ... Since Liberty-Link™ HEAR lines have been supported for registration in Canada, commercial production of Liberty-Link™ HEAR cultivars could start in the near future. ...
A hybrid developed from a cross between two diverse Brassica napus cultivars ("Polo" and "Topas")... more A hybrid developed from a cross between two diverse Brassica napus cultivars ("Polo" and "Topas") was used to produce a microspore derived double haploid (DH) population and a single seed descent derived recombinant inbred (RI) population for genetic mapping. Each of the two populations consisting of 190 DH lines and 94 RI lines was characterized for various types (SSR, SRAP, ISSR, SCAR) of polymorphic molecular markers. The DH population was scored for 620 molecular markers while the RI population was scored for 349 molecular markers to construct two independent genetic maps. In both genetic maps, all of the molecular markers were found to cluster in 19 linkage groups (LGs) covered a total genome length of 2244.1 cM and 1649.1 cM for the DH and RI maps, respectively. The data from the two genetic maps was used to construct a consensus integrated genetic map covering a total genome length of 2464.9 cM. Previously published Brassica reference genetic maps were used to assign each of the nineteen LGs to corresponding Brassica napus chromosomes named N01 to N19. To our knowledge, this is the first integrated genetic map based on DH and RI populations developed from the same cross in Brassica napus.
Four genotypes of B. napus (cultivars Topas, Sentry and Polo, and the experimental line 04C204) v... more Four genotypes of B. napus (cultivars Topas, Sentry and Polo, and the experimental line 04C204) varying in seed oil content (SOC) potential (from 42.2% to 50.0% seed DW) were used to study C and N partitioning among shoots, roots and soil, as well as nitrogen use efficiency (NUE). Topas, with lowest SOC potential, had higher biomass accumulation in shoots and roots and higher NUE than 04C204 with the highest SOC potential. Although the absolute amounts of biomass and N accumulated in Topas were greater than in 04C204, the percentage of 13 C partitioned to shoots was lower, and the percentage of 15 N partitioned to roots was higher. These results imply that SOC may be more associated with the proportions of C and N partitioned between shoots and roots, than with absolute amounts accumulated in these organs. This study also indicates that while Topas fixed the most CO 2 , it also had the greatest nitrogen fertilizer demand, while 04C204, with the highest SOC potential, had the greatest proportion of whole plant C retained within shoots of all the genotypes. These results suggest that a breeding strategy to optimize B. napus as a biodiesel feedstock may well be selection for maximum SOC at a minimal investment in vegetative biomass (and hence a lower N fertilizer requirement).
McVetty, P. B. E., Duncan, R. W., Fernando, W. G. D., Li, G. and Zelmer, C. D. 2012. Red River 18... more McVetty, P. B. E., Duncan, R. W., Fernando, W. G. D., Li, G. and Zelmer, C. D. 2012. Red River 1861 Roundup Ready™ high erucic acid, low glucosinolate summer rape. Can. J. Plant Sci. 92: 1407–1409. Red River 1861 summer rape (Brassica napus L.) is the world's fourth Roundup Ready™ high erucic acid, low glucosinolate cultivar. On average, Red River 1861 yielded 19% more seed, 7 g kg−1 more seed oil and 7 g kg −1 more meal protein than MillenniUM 03 high erucic acid, low glucosinolate summer rape. Red River 1861 has an erucic acid content of 52.6% in isolated field trials of HEAR lines and is adapted to the southern B. napus growing regions of western Canada.
Background: Sequence related amplified polymorphism (SRAP) is commonly used to construct high den... more Background: Sequence related amplified polymorphism (SRAP) is commonly used to construct high density genetic maps, map genes and QTL of important agronomic traits in crops and perform genetic diversity analysis without knowing sequence information. To combine next generation sequencing technology with SRAP, Illumina’s Solexa sequencing was used to sequence tagged SRAP PCR products. Results: Three sets of SRAP primers and three sets of tagging primers were used in 77,568 SRAP PCR reactions and the same number of tagging PCR reactions respectively to produce a pooled sample for Illumina’s Solexa sequencing. After sequencing, 1.28 GB of sequence with over 13 million paired-end sequences was obtained and used to match Solexa sequences with their corresponding SRAP markers and to integrate Solexa sequences on an ultradense genetic map. The ultradense genetic bin map with 465 bins was constructed using a recombinant inbred (RI) line mapping population in B. rapa. For this ultradense gene...
The effect of changing the oxygen concentration from 21 to 2% on photosynthesis of wheat, sunflow... more The effect of changing the oxygen concentration from 21 to 2% on photosynthesis of wheat, sunflower, and soybean was investigated. At low CO2 concentrations and low light intensities, a stimulation of photosynthesis was observed in 2% oxygen compared with the rate in 21% O2. At high CO2 concentrations and high light intensities, a temporary inhibition of photosynthesis was observed when the oxygen concentration was changed from 21 to 2%. In wheat and sunflower, this inhibition was observed at progressively lower CO2 concentrations as temperatures were decreased. In soybean only, a slight inhibition of photosynthesis was observed at higher temperatures. In some cases, especially in plants grown under a low light intensity, a long-lasting (> 45 min) inhibition of photosynthesis was observed. In most cases, however, the inhibition lasted only for several minutes and final rates of photosynthesis in 2% O2 were equal to or greater than the rate of photosynthesis in 21% O2. The stimula...
Clubroot disease is devastating to Brassica crop production when susceptible cultivars are plante... more Clubroot disease is devastating to Brassica crop production when susceptible cultivars are planted in infected fields. European turnips are the most resistant sources and their resistance genes have been introduced into other crops such oilseed rape (Brassica napus L.), Chinese cabbage and other Brassica vegetables. The European clubroot differential (ECD) set contains four turnip accessions (ECD1-4). These ECD turnips exhibited high levels of resistance to clubroot when they were tested under controlled environmental conditions with Canadian field isolates. Gene mapping of the clubroot resistance genes in ECD1-4 were performed and three independent dominant resistance loci were identified. Two resistance loci were mapped on chromosome A03 and the third on chromosome A08. Each ECD turnip accession contained two of these three resistance loci. Some resistance loci were homozygous in ECD accessions while others showed heterozygosity based on the segregation of clubroot resistance in 20 BC 1 families derived from ECD1 to 4. Molecular markers were developed linked to each clubroot resistance loci for the resistance gene introgression in different germplasm.
Evolve is the world’s first ogu INRA CMS hybrid summer rape (Brassica napus L.) Roundup Ready® hi... more Evolve is the world’s first ogu INRA CMS hybrid summer rape (Brassica napus L.) Roundup Ready® high erucic acid, low glucosinolate cultivar. On average, Evolve yielded 12.4 % more seed, 6 g kg-1 more seed oil and 5 g kg -1 less meal protein than Red River 1861, an open-pollinated Roundup Ready® high erucic acid, low glucosinolate summer rape. Evolve has an erucic acid content of 52.3 % in isolated field trials of HEAR genotypes and is adapted to the southern B. napus growing regions of western Canada.
Journal of the American Society for Horticultural Science
There are various clubroot pathogen (Plasmodiophora brassicae) resistance genes within Brassica s... more There are various clubroot pathogen (Plasmodiophora brassicae) resistance genes within Brassica species with european turnip (B. rapa ssp. rapifera) being identified as potentially the best source of resistance for the development of clubroot-resistant cultivars in chinese cabbage (B. rapa ssp. pekinensis). To use clubroot resistance genes effectively, it is necessary to map these genes so that molecular markers inside or closely linked to these resistance genes can be developed. Using molecular marker-assisted selection, the clubroot resistance genes can be effectively transferred from cultivar to cultivar and from species to species. In this report, one clubroot resistance locus was mapped on linkage group A3 using five segregating populations developed from five chinese cabbage cultivars, suggesting that all the five cultivars shared the same clubroot resistance locus. Furthermore, one of these five chinese cabbage cultivars was used to develop a large segregating population to f...
HYHEAR 3 summer rape (Brassica napus L.) is a Roundup Ready®, high erucic acid, low glucosinolate... more HYHEAR 3 summer rape (Brassica napus L.) is a Roundup Ready®, high erucic acid, low glucosinolate cultivar. On average, HYHEAR 3 yielded 15.6% more seed, 4 g kg −1 less seed oil, and 22 g kg −1 less meal protein than Red River 1861 Roundup Ready®, high erucic acid, low glucosinolate summer rape. HYHEAR 3 has an erucic acid content of 50.9% in isolated field trials of high erucic acid rapeseed (HEAR) genotypes and is adapted to the southern B. napus growing regions of western Canada.
The growth of all grass-clump dwarfs is sensitive to temperature with low temperature giving rise... more The growth of all grass-clump dwarfs is sensitive to temperature with low temperature giving rise to the grass-clump phenotype and high temperature producing normal phenotype. A continuous temperature of 26°C is required for normal growth of Type 1 dwarfs, a continuous temperature of 21 °C is required for normal growth of Type 2 dwarfs and a continuous temperature of 16°C is required for normal growth of Type 3 dwarfs. Genetic studies show that the inheritance of the grass-clump characteristic is due to three complementary dominant genes. The grass-clump growth habit is produced as a result of the temperature sensitivity of the apical meristem. In grass-clump plants low temperature treatment results in the cessation of cell division, DNA synthesis and phospholipid synthesis in the apical meristem. The primary temperature lesion has not been identified. Prolonged low temperature treatment of grass-clump plants results in extensive cell necrosis in a region just below the apical meristem; this cell death results in the permanent inactivation of the apical meristem.
Technological Innovations in Major World Oil Crops, Volume 2, 2011
... Applications in Oilseed Rape and Linseed Peter BE McVetty and Rachael Scarth ... Since Libert... more ... Applications in Oilseed Rape and Linseed Peter BE McVetty and Rachael Scarth ... Since Liberty-Link™ HEAR lines have been supported for registration in Canada, commercial production of Liberty-Link™ HEAR cultivars could start in the near future. ...
A hybrid developed from a cross between two diverse Brassica napus cultivars ("Polo" and "Topas")... more A hybrid developed from a cross between two diverse Brassica napus cultivars ("Polo" and "Topas") was used to produce a microspore derived double haploid (DH) population and a single seed descent derived recombinant inbred (RI) population for genetic mapping. Each of the two populations consisting of 190 DH lines and 94 RI lines was characterized for various types (SSR, SRAP, ISSR, SCAR) of polymorphic molecular markers. The DH population was scored for 620 molecular markers while the RI population was scored for 349 molecular markers to construct two independent genetic maps. In both genetic maps, all of the molecular markers were found to cluster in 19 linkage groups (LGs) covered a total genome length of 2244.1 cM and 1649.1 cM for the DH and RI maps, respectively. The data from the two genetic maps was used to construct a consensus integrated genetic map covering a total genome length of 2464.9 cM. Previously published Brassica reference genetic maps were used to assign each of the nineteen LGs to corresponding Brassica napus chromosomes named N01 to N19. To our knowledge, this is the first integrated genetic map based on DH and RI populations developed from the same cross in Brassica napus.
Four genotypes of B. napus (cultivars Topas, Sentry and Polo, and the experimental line 04C204) v... more Four genotypes of B. napus (cultivars Topas, Sentry and Polo, and the experimental line 04C204) varying in seed oil content (SOC) potential (from 42.2% to 50.0% seed DW) were used to study C and N partitioning among shoots, roots and soil, as well as nitrogen use efficiency (NUE). Topas, with lowest SOC potential, had higher biomass accumulation in shoots and roots and higher NUE than 04C204 with the highest SOC potential. Although the absolute amounts of biomass and N accumulated in Topas were greater than in 04C204, the percentage of 13 C partitioned to shoots was lower, and the percentage of 15 N partitioned to roots was higher. These results imply that SOC may be more associated with the proportions of C and N partitioned between shoots and roots, than with absolute amounts accumulated in these organs. This study also indicates that while Topas fixed the most CO 2 , it also had the greatest nitrogen fertilizer demand, while 04C204, with the highest SOC potential, had the greatest proportion of whole plant C retained within shoots of all the genotypes. These results suggest that a breeding strategy to optimize B. napus as a biodiesel feedstock may well be selection for maximum SOC at a minimal investment in vegetative biomass (and hence a lower N fertilizer requirement).
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