So schwer war das gar nicht. Jedenfalls hatte ich Schlimmeres erwartet. Also tatsächlich war es e... more So schwer war das gar nicht. Jedenfalls hatte ich Schlimmeres erwartet. Also tatsächlich war es eigentlich ein Kinderspiel. Man musste sich nur ein paar Gedanken machen. Das reichte. Scheint so, dass die meisten Sicherheitsleute denken, wenn man Router und Firewalls und Intrusion Detection Systeme (IDS) einsetzt, dann haben sie ihr Netzwerk abgesichert. Aber das trifft nicht notwendigerweise zu. Es braucht nur eine klitzekleine Fehlkonfiguration irgendwo im Netzwerk oder auf einem Server, damit einer durch diese Lücke schlüpfen kann …
For the first time, each specificity determining residue (SDR) in the binding site of an antibody... more For the first time, each specificity determining residue (SDR) in the binding site of an antibody has been replaced with every other possible single amino acid substitution, and the resulting mutants analyzed for binding affinity and specificity. The studies were conducted on a variant of the 26-10 antidigoxin single chain Fv (scFv) using in vitro scanning saturation mutagenesis, a new process that allows the high throughput production and characterization of antibody mutants [Burks,E.A., Chen,G., Georgiou,G. and Iverson,B.L. (1997) Proc. Natl Acad. Sci. USA, 94, 412-417]. Single amino acid mutants of 26-10 scFv were identified that modulated specificity in dramatic fashion. The overall plasticity of the antibody binding site with respect to amino acid replacement was also evaluated, revealing that 86% of all mutants retained measurable binding activity. Finally, by analyzing the physical properties of amino acid substitutions with respect to their effect on hapten binding, conclusions were drawn regarding the functional role played by the wild-type residue at each SDR position. The reported results highlight the value of in vitro scanning saturation mutagenesis for engineering antibody binding specificity, for evaluating the plasticity of proteins, and for comprehensive structure-function studies and analysis. by guest on May 2, 2013 http://peds.oxfordjournals.org/ Downloaded from Gang Chen et al.
Firewalls provide a variety of services to networks in terms of security. They provide for networ... more Firewalls provide a variety of services to networks in terms of security. They provide for network address tr networks (VPN), and filtering of traffic that does not conform to the network's stated security policy. There simple packet filters to circuit-level gateways to proxy firewalls. Firewalls are being asked to fill a larger and security these days than several years ago. One of the more recent innovations in firewall technology is the inspection or DPI. Deep Packet Inspection can be seen as the integration of Intrusion Detection (IDS) and I capabilities with traditional stateful firewall technology. Traditional networks have a defined boundary dema
ABSTRACT The authors describe their experiences at a major space engineering university (the Univ... more ABSTRACT The authors describe their experiences at a major space engineering university (the University of Texas at Austin) in the use of graduate level design courses and projects to produce information and tools that are of use to undergraduate design classes, graduate students, and industry. The information produced to date includes a spacecraft subsystems information document, a mission design tool (a FORTRAN subroutine library), a series of space mission characterizations, and a set of spacecraft characterizations.
So schwer war das gar nicht. Jedenfalls hatte ich Schlimmeres erwartet. Also tatsächlich war es e... more So schwer war das gar nicht. Jedenfalls hatte ich Schlimmeres erwartet. Also tatsächlich war es eigentlich ein Kinderspiel. Man musste sich nur ein paar Gedanken machen. Das reichte. Scheint so, dass die meisten Sicherheitsleute denken, wenn man Router und Firewalls und Intrusion Detection Systeme (IDS) einsetzt, dann haben sie ihr Netzwerk abgesichert. Aber das trifft nicht notwendigerweise zu. Es braucht nur eine klitzekleine Fehlkonfiguration irgendwo im Netzwerk oder auf einem Server, damit einer durch diese Lücke schlüpfen kann …
For the first time, each specificity determining residue (SDR) in the binding site of an antibody... more For the first time, each specificity determining residue (SDR) in the binding site of an antibody has been replaced with every other possible single amino acid substitution, and the resulting mutants analyzed for binding affinity and specificity. The studies were conducted on a variant of the 26-10 antidigoxin single chain Fv (scFv) using in vitro scanning saturation mutagenesis, a new process that allows the high throughput production and characterization of antibody mutants [Burks,E.A., Chen,G., Georgiou,G. and Iverson,B.L. (1997) Proc. Natl Acad. Sci. USA, 94, 412-417]. Single amino acid mutants of 26-10 scFv were identified that modulated specificity in dramatic fashion. The overall plasticity of the antibody binding site with respect to amino acid replacement was also evaluated, revealing that 86% of all mutants retained measurable binding activity. Finally, by analyzing the physical properties of amino acid substitutions with respect to their effect on hapten binding, conclusions were drawn regarding the functional role played by the wild-type residue at each SDR position. The reported results highlight the value of in vitro scanning saturation mutagenesis for engineering antibody binding specificity, for evaluating the plasticity of proteins, and for comprehensive structure-function studies and analysis. by guest on May 2, 2013 http://peds.oxfordjournals.org/ Downloaded from Gang Chen et al.
Firewalls provide a variety of services to networks in terms of security. They provide for networ... more Firewalls provide a variety of services to networks in terms of security. They provide for network address tr networks (VPN), and filtering of traffic that does not conform to the network's stated security policy. There simple packet filters to circuit-level gateways to proxy firewalls. Firewalls are being asked to fill a larger and security these days than several years ago. One of the more recent innovations in firewall technology is the inspection or DPI. Deep Packet Inspection can be seen as the integration of Intrusion Detection (IDS) and I capabilities with traditional stateful firewall technology. Traditional networks have a defined boundary dema
ABSTRACT The authors describe their experiences at a major space engineering university (the Univ... more ABSTRACT The authors describe their experiences at a major space engineering university (the University of Texas at Austin) in the use of graduate level design courses and projects to produce information and tools that are of use to undergraduate design classes, graduate students, and industry. The information produced to date includes a spacecraft subsystems information document, a mission design tool (a FORTRAN subroutine library), a series of space mission characterizations, and a set of spacecraft characterizations.
Uploads
Papers by Ido Dubrawsky