Anaplastic thyroid cancer (ATC) is the rarest (1-2%) form of thyroid cancer, but also the most ag... more Anaplastic thyroid cancer (ATC) is the rarest (1-2%) form of thyroid cancer, but also the most aggressive and associated with the worst prognosis. The survival median rate is 5-6 months, whereas only 20% of patients survive more than one year from the diagnosis, even though the usage of radiotherapy and surgical resection. The growing incidence rate of thyroid cancer and ATC determine the need for new prophylactic, diagnostic and therapeutic solutions. Metformin was first introduced as an oral antidiabetic drug. The beneficial effect of metformin on anaplastic thyroid cancer cells was confirmed, however, the mechanism of this interaction is still unclear. The usage of metformin in thyroid cancer prevention is still under discussion-nevertheless, studies conducted on larger groups support this beneficial impact, at least in patients with insulin resistance or metabolic syndrome. Both the synergistic effect of metformin in anaplastic thyroid cancer chemotherapy and its protective effect in radiotherapy are still concerns and need additional confirmation in randomized clinical trials. This review aims to sum up the recent knowledge on metformin usage in ATC.
Preeclampsia and hypertension complicate several pregnancies. Identifying women at risk of develo... more Preeclampsia and hypertension complicate several pregnancies. Identifying women at risk of developing these conditions is essential to establish potential treatment modalities. Biomarkers such as C19MC microRNA in pregnant patients wopuld assist in defining pregnancy surveillance and implementing interventions. This study sought to analyze circulating C19MC microRNA as an early marker of hypertension and preeclampsia in pregnant patients. A systematic review was undertaken using the following registers: disease registries, pregnancy registries, and pregnancy exposure registries, and the following databases: PubMed, CINAHL, Web of Science, Scopus, and EMBASE. The risk of bias was assessed using the Cochrane technique. From the 45 publications retrieved from the registers and databases, only 21 were included in the review after the removal of duplicates, screening, and eligibility evaluation. All 210 publications had a low risk of bias and illuminated the potential use of circulating ...
Pre-eclampsia is a placenta-related complication occurring in 2–10% of all pregnancies. miRNAs ar... more Pre-eclampsia is a placenta-related complication occurring in 2–10% of all pregnancies. miRNAs are a group of non-coding RNAs regulating gene expression. There is evidence that C19MC miRNAs are involved in the development of the placenta. Deregulation of chromosome 19 microRNA cluster (C19MC) miRNAs expression leads to impaired cell differentiation, abnormal trophoblast invasion and pathological angiogenesis, which can lead to the development of pre-eclampsia. Information was obtained through a review of articles available in PubMed Medline. Articles on the role of the C19MC miRNA in the development of pre-eclampsia published in 2009–2022 were analyzed. This review article summarizes the current data on the role of the C19MC miRNA in the development of pre-eclampsia. They indicate a significant increase in the expression of most C19MC miRNAs in placental tissue and a high level of circulating fractions in serum and plasma, both in the first and/or third trimester in women with PE. O...
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Aromatic L-amino acid decarboxylase deficiency is an autosomal recessive neurodevelopmental disor... more Aromatic L-amino acid decarboxylase deficiency is an autosomal recessive neurodevelopmental disorder caused by pathogenic variants of the DDC gene. The disease manifests already in newborns and infants. The presentation includes neurological symptoms, a significant delay in motor development and oculogyric crisis. Currently, gene therapy is successfully used in the treatment of aromatic L-amino acid decarboxylase deficiency. Until recently, no effective treatment for the disorder was known. The affected children died in the first decades of life. Gene therapy is a new and promising therapeutic strategy. The first genetic therapies for aromatic L-amino acid decarboxylase deficiency were implemented in the United States. The treated children recovered very quickly, began to sit up, stand, and even attempted to walk. For the first time in Europe, this method was used in 2019 in Poland, at the Interventional NeuroTherapy Centre at Bródno Hospital in Warsaw, with the involvement of a tea...
BackgroundThe most popular tool used for measuring personality traits is the Five-Factor Model (F... more BackgroundThe most popular tool used for measuring personality traits is the Five-Factor Model (FFM). It includes neuroticism, extraversion, openness, agreeableness and conscientiousness. Many studies indicated the association of genes encoding neurotransmitter receptors/transporters with personality traits. The relationship connecting polymorphic DNA sequences and FFM features has been described in the case of genes encoding receptors of cannabinoid and dopaminergic systems. Moreover, dopaminergic system receives inputs from other neurotransmitters, like GABAergic or serotoninergic systems.MethodsWe searched PubMed Central (PMC), Science Direct, Scopus, Cochrane Library, Web of Science and EBSCO databases from their inception to November 19, 2020, to identify original studies, as well as peer-reviewed studies examining the FFM and its association with gene polymorphisms affecting the neurotransmitter functions in central nervous system.ResultsSerotonin neurons modulate dopamine fun...
Introduction. Multiple myeloma (MM) is a hematological malignancy characterized by genetic variet... more Introduction. Multiple myeloma (MM) is a hematological malignancy characterized by genetic variety. The 3020insC variant of the NOD2/CARD15 gene results in the upregulation of proinflammatory cytokines. Chronic inflammation and abnormal function of the proteasome system may lead to MM development. The polymorphism (-8C>G) in the PSMA6 gene affects proteasome activity. The aim of our study was to analyze the possible relationship of NOD/CARD15 and PSMA6 genes with the risk of development and outcome of MM, as well as the sensitivity to bortezomib (proteasome inhibitor) in cell cultures derived from MM patients. Objects and Methods. Genomic DNA from 100 newly diagnosed MM patients and 100 healthy blood donors was analyzed by methods such as PCR-RFLP (for PSMA6 genotyping) and automated DNA sequencing (for NOD2/CARD15 genotyping). In a subgroup of 50 MM patients, nucleated bone marrow cells were treated with bortezomib in vitro. Results. Patients with PSMA6 CG+GG genotypes had highe...
Antioxidant compounds such as glutathione and its enzymes have become the focus of attention of m... more Antioxidant compounds such as glutathione and its enzymes have become the focus of attention of medical sciences. Glutathione, a specific tripeptide, is involved in many intercellular processes. The glutathione concentration is determined by the number of GAG repeats in gamma-glutamylcysteine synthetase. GAG polymorphisms are associated with an increased risk of schizophrenia, berylliosis, diabetes, lung cancer, and nasopharyngeal tumors. Cancer cells with high glutathione concentration are resistant to chemotherapy treatment. The oxidized form of glutathione is formed by glutathione peroxidases (GPXs). The changes in activity of GPX1, GPX2, and GPX3 isoforms may be associated with the development of cancers, for example, prostate cancer or even colon cancer. Detoxification of glutathione conjugates is possible due to activity of glutathione S-transferases (GSTs). Polymorphisms in GSTM1, GSTP1, and GSTO1 enzymes increase the risk of developing breast cancer and hepatocellular carcin...
Introduction. NOTCH pathway and TP53 protein are involved in the development of fibrosis and auto... more Introduction. NOTCH pathway and TP53 protein are involved in the development of fibrosis and autoimmune disorders, respectively. The aim of this study was to evaluate the role of single nucleotide polymorphisms (SNPs) of NOTCH3 and TP53 genes and serum anti-TP53 antibodies with the susceptibility, clinical subset of systemic sclerosis (SSc), and clinical profile of SSc patient, particularly with lung involvement and disease activity. Objects and Methods. 124 white Polish SSc patients (101 with limited cutaneous SSc–lcSSc, and 23 with diffuse cutaneous SSc–dcSSc) and 100 healthy individuals were included in the study. Patients were assessed for the presence of autoantibodies and interstitial lung disease. Two SNPs at position 6746 of NOTCH3 gene (C/T alleles) and 215 of the TP53 gene (P/R alleles) were genotyped by PCR-restriction fragment length polymorphism. Serum levels of anti-TP53 antibodies were analyzed by means of ELISA. Results. The genotypic frequencies of the NOTCH3 gene f...
Objectives: MicroRNAs have been observed to play a major role in various physiological processes,... more Objectives: MicroRNAs have been observed to play a major role in various physiological processes, for instance, programmed cell death, cell division, pregnancy development, and proliferation. With the help of profiling of microRNAs in the serum of pregnant women, it is possible to link alterations in their concentration to the emergence of gestational problems. The aim of the study was to evaluate the diagnostic potential of MicroRNAs Mi 517 and Mi 526 as biomarkers in the detection of hypertension and preeclampsia. Material and methods: The study considered 53 patients who are in their first trimester of a singleton pregnancy. Participants have been divided into two study groups, one group with normal pregnancy and another group having the risk of developing preeclampsia or who developed hypertension or preeclampsia during follow-up constitute the study group. In order to collect data associated with circulating miRNAs in serum, blood samples have been collected from the participants of the study. Results: Based on the univariate regression model, increased expression of Mi 517 and 526 and parity status (primapara/multipara) has been obtained. The multivariate logistic analysis shows that independent risk factors for hypertension or preeclampsia are the presence of an R527 and being a primipara. Conclusions: The study's findings have revealed that R517s and R526s act as major indicative biomarkers in the first trimester for the detection of hypertension and preeclampsia. The circulating C19MC MicroRNA was examined as a potential early indicator of preeclampsia and hypertension in pregnant individuals.
This study aimed to evaluate the safety and potential use of soy isoflavones in the treatment of ... more This study aimed to evaluate the safety and potential use of soy isoflavones in the treatment of skin problems, difficult-to-heal wounds and postoperative scars in women after the oncological treatment of breast cancer. The effects of different concentrations of genistein as a representative of soy isoflavonoids on MCF-7 tumor cells and BJ skin fibroblasts cultured in vitro were assessed. Genistein affects both healthy dermal BJ fibroblasts and cancerous MCF-7 cells. The effect of the tested isoflavonoid is closely related to its concentration. High concentrations of genistein destroy MCF-7 cancer cells, regardless of the exposure time, with a much greater effect on reducing cancer cell numbers at longer times (48 h). Lower concentrations of genistein (10 and 20 μM) increase the abundance of dermal fibroblasts. However, higher concentrations of genistein (50 μM and higher) are detrimental to fibroblasts at longer exposure times (48 h). Our studies indicate that although genistein sh...
alterations in microrna (mirna/mirs) expression are associated with the occurrence and course of ... more alterations in microrna (mirna/mirs) expression are associated with the occurrence and course of human diseases, including chronic lymphocytic leukemia (cll). expression of mirnas may vary among patients with cll in different cytogenetic risk groups. The present study assessed the expression levels of the following mirnas in 35 patients with cll: hsa-mir-15a,-16-1,-29a,-29c,-34a,-34b,-155,-181a,-181b,-221,-222 and-223. Fluorescent in situ hybridization (FiSH) analysis was performed for 13q14d, 17p13 and 11q22 deletions and chromosome 12 trisomy. Significantly higher expression levels of mir-181a,-221 and-223 were observed in the group at low risk of disease progression (stage 0) compared with the group with high risk of cll progression (P=0.036, P=0.019 and P=0.038, respectively). The present study revealed that the expression levels of mirna-181b and miRNA-223 were significantly higher in the group of patients without d13S319 deletion (P=0.039 and P=0.037, respectively). Moreover, the expression levels of mir-15a and mirna-29c were demonstrated to be significantly higher in the group of patients with cll who had a tumor protein p53 deletion, identified by FISH, compared with patients without this lesion (P=0.047, P=0.03 respectively). Based on receiver operating characteristic curve analysis, the present study revealed that mir-181a,-221 and-223 expression was able to distinguish low and high risk of cll progression in patients. among the tested mirnas, mirna-181a,-221 and-223 were indicated to have the greatest diagnostic potential in cll.
Despite significant medical advances, cancer treatment is still associated with a high risk of si... more Despite significant medical advances, cancer treatment is still associated with a high risk of side effects. The treatment is usually invasive and devastating and it affects the overall immunity of the whole organism, including the condition of the skin. In recent years there has been a growing interest in isoflavonoids, due to their wide range of biological and pharmacological activity, especially estrogen-like. It gives a broad perspective of their use as active ingredients of preparations, which eliminate skin lesions associated with oncological treatment. This article is an overview describing preclinical and clinical observations on the basis of available literature. It discusses the influence of genistein on skin health in women after breast cancer treatment. The overview focuses on studies conducted with genistein in vitro or in vivo to demonstrate its effect on skin, and anticancer properties. We selected articles from the last 20 years, available in the PubMed and Google Scholar databases.
Background: The aim of the current study is to analyze and summarize the latest research on impro... more Background: The aim of the current study is to analyze and summarize the latest research on improving therapy in ovarian cancer. Materials & methods: Data analysis was based on a review of publications from 2011 to 2021 in the PubMed database with use of the search terms including ‘EGFR ovarian cancer’, ‘folate receptor inhibitors ovarian cancer’, ‘VEGF ovarian cancer’, ‘PDGF ovarian cancer’ and ‘CTLA-4 ovarian cancer’. Results: 6643 articles were found; 238 clinical trials and randomized control trials were analyzed; 122 studies were rejected due to inconsistency with the topic of the work. Conclusion: Extensive research on the treatment of ovarian cancer increases the chance of developing the most effective therapy suited to the individual needs of the patient.
Oncology is one of the most rapidly developing fields in medicinal studies, however, it still can... more Oncology is one of the most rapidly developing fields in medicinal studies, however, it still cannot provide therapies that are efficient enough in neoplasms. As both morbidity and mortality of cancerous diseases increase annually, it is necessary to improve diagnostic and therapeutic procedures. One of the most promising areas is nanomedicine. The aim of the study is to review current therapeutic and diagnostic methods that involve nanotechnology. Methods Materials shared by the National Cancer Institute and research articles available in the PubMed database, that were searched by keywords "nanoparticle" and "cancer", were analyzed. Additionally, a search of clinical randomized trials was refined to a period over the last 5 years. Results: Over the last 5 years, studies on the use of nanoparticles in the treatment of breast cancer, thyroid cancer, pancreatic cancer, non-small cellular lung cancer, hepatocellular carcinoma, soft tissue sarcoma, cervical cancer, a...
Rak jajnika od lat jest przedmiotem szczególnego zainteresowania klinicystów i naukowców na całym... more Rak jajnika od lat jest przedmiotem szczególnego zainteresowania klinicystów i naukowców na całym świecie. Badania przeprowadzone w ostatnich latach w grupie pacjentek z rakiem jajnika wskazują na charakterystyczne podłoże konstytucyjne sprzyjające rozwojowi tego nowotworu. Na podstawie badań z zakresu genetyki wykazano, że zaburzenia w genach BRCA1, BRCA2 predysponują do rozwoju raka jajnika. Celem pracy była ocena mutacji genu BRCA1 u kobiety chorej na raka jajnika. W badaniu zastosowano metodę studium przypadku. Na podstawie oceny badania genetycznego stwierdzono, że obecność mutacji w genie BRCA1 była czynnikiem ryzyka wystąpienia nowotworu jajnika u badanej pacjentki. Edukacja kobiet w zakresie możliwości diagnostycznych i metod prewencji jest niezbędna dla uniknięcia raka jajnika lub zwiększenia szans na przeżycie w przypadku jego rozwoju.
Anaplastic thyroid cancer (ATC) is the rarest (1-2%) form of thyroid cancer, but also the most ag... more Anaplastic thyroid cancer (ATC) is the rarest (1-2%) form of thyroid cancer, but also the most aggressive and associated with the worst prognosis. The survival median rate is 5-6 months, whereas only 20% of patients survive more than one year from the diagnosis, even though the usage of radiotherapy and surgical resection. The growing incidence rate of thyroid cancer and ATC determine the need for new prophylactic, diagnostic and therapeutic solutions. Metformin was first introduced as an oral antidiabetic drug. The beneficial effect of metformin on anaplastic thyroid cancer cells was confirmed, however, the mechanism of this interaction is still unclear. The usage of metformin in thyroid cancer prevention is still under discussion-nevertheless, studies conducted on larger groups support this beneficial impact, at least in patients with insulin resistance or metabolic syndrome. Both the synergistic effect of metformin in anaplastic thyroid cancer chemotherapy and its protective effect in radiotherapy are still concerns and need additional confirmation in randomized clinical trials. This review aims to sum up the recent knowledge on metformin usage in ATC.
Preeclampsia and hypertension complicate several pregnancies. Identifying women at risk of develo... more Preeclampsia and hypertension complicate several pregnancies. Identifying women at risk of developing these conditions is essential to establish potential treatment modalities. Biomarkers such as C19MC microRNA in pregnant patients wopuld assist in defining pregnancy surveillance and implementing interventions. This study sought to analyze circulating C19MC microRNA as an early marker of hypertension and preeclampsia in pregnant patients. A systematic review was undertaken using the following registers: disease registries, pregnancy registries, and pregnancy exposure registries, and the following databases: PubMed, CINAHL, Web of Science, Scopus, and EMBASE. The risk of bias was assessed using the Cochrane technique. From the 45 publications retrieved from the registers and databases, only 21 were included in the review after the removal of duplicates, screening, and eligibility evaluation. All 210 publications had a low risk of bias and illuminated the potential use of circulating ...
Pre-eclampsia is a placenta-related complication occurring in 2–10% of all pregnancies. miRNAs ar... more Pre-eclampsia is a placenta-related complication occurring in 2–10% of all pregnancies. miRNAs are a group of non-coding RNAs regulating gene expression. There is evidence that C19MC miRNAs are involved in the development of the placenta. Deregulation of chromosome 19 microRNA cluster (C19MC) miRNAs expression leads to impaired cell differentiation, abnormal trophoblast invasion and pathological angiogenesis, which can lead to the development of pre-eclampsia. Information was obtained through a review of articles available in PubMed Medline. Articles on the role of the C19MC miRNA in the development of pre-eclampsia published in 2009–2022 were analyzed. This review article summarizes the current data on the role of the C19MC miRNA in the development of pre-eclampsia. They indicate a significant increase in the expression of most C19MC miRNAs in placental tissue and a high level of circulating fractions in serum and plasma, both in the first and/or third trimester in women with PE. O...
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Aromatic L-amino acid decarboxylase deficiency is an autosomal recessive neurodevelopmental disor... more Aromatic L-amino acid decarboxylase deficiency is an autosomal recessive neurodevelopmental disorder caused by pathogenic variants of the DDC gene. The disease manifests already in newborns and infants. The presentation includes neurological symptoms, a significant delay in motor development and oculogyric crisis. Currently, gene therapy is successfully used in the treatment of aromatic L-amino acid decarboxylase deficiency. Until recently, no effective treatment for the disorder was known. The affected children died in the first decades of life. Gene therapy is a new and promising therapeutic strategy. The first genetic therapies for aromatic L-amino acid decarboxylase deficiency were implemented in the United States. The treated children recovered very quickly, began to sit up, stand, and even attempted to walk. For the first time in Europe, this method was used in 2019 in Poland, at the Interventional NeuroTherapy Centre at Bródno Hospital in Warsaw, with the involvement of a tea...
BackgroundThe most popular tool used for measuring personality traits is the Five-Factor Model (F... more BackgroundThe most popular tool used for measuring personality traits is the Five-Factor Model (FFM). It includes neuroticism, extraversion, openness, agreeableness and conscientiousness. Many studies indicated the association of genes encoding neurotransmitter receptors/transporters with personality traits. The relationship connecting polymorphic DNA sequences and FFM features has been described in the case of genes encoding receptors of cannabinoid and dopaminergic systems. Moreover, dopaminergic system receives inputs from other neurotransmitters, like GABAergic or serotoninergic systems.MethodsWe searched PubMed Central (PMC), Science Direct, Scopus, Cochrane Library, Web of Science and EBSCO databases from their inception to November 19, 2020, to identify original studies, as well as peer-reviewed studies examining the FFM and its association with gene polymorphisms affecting the neurotransmitter functions in central nervous system.ResultsSerotonin neurons modulate dopamine fun...
Introduction. Multiple myeloma (MM) is a hematological malignancy characterized by genetic variet... more Introduction. Multiple myeloma (MM) is a hematological malignancy characterized by genetic variety. The 3020insC variant of the NOD2/CARD15 gene results in the upregulation of proinflammatory cytokines. Chronic inflammation and abnormal function of the proteasome system may lead to MM development. The polymorphism (-8C>G) in the PSMA6 gene affects proteasome activity. The aim of our study was to analyze the possible relationship of NOD/CARD15 and PSMA6 genes with the risk of development and outcome of MM, as well as the sensitivity to bortezomib (proteasome inhibitor) in cell cultures derived from MM patients. Objects and Methods. Genomic DNA from 100 newly diagnosed MM patients and 100 healthy blood donors was analyzed by methods such as PCR-RFLP (for PSMA6 genotyping) and automated DNA sequencing (for NOD2/CARD15 genotyping). In a subgroup of 50 MM patients, nucleated bone marrow cells were treated with bortezomib in vitro. Results. Patients with PSMA6 CG+GG genotypes had highe...
Antioxidant compounds such as glutathione and its enzymes have become the focus of attention of m... more Antioxidant compounds such as glutathione and its enzymes have become the focus of attention of medical sciences. Glutathione, a specific tripeptide, is involved in many intercellular processes. The glutathione concentration is determined by the number of GAG repeats in gamma-glutamylcysteine synthetase. GAG polymorphisms are associated with an increased risk of schizophrenia, berylliosis, diabetes, lung cancer, and nasopharyngeal tumors. Cancer cells with high glutathione concentration are resistant to chemotherapy treatment. The oxidized form of glutathione is formed by glutathione peroxidases (GPXs). The changes in activity of GPX1, GPX2, and GPX3 isoforms may be associated with the development of cancers, for example, prostate cancer or even colon cancer. Detoxification of glutathione conjugates is possible due to activity of glutathione S-transferases (GSTs). Polymorphisms in GSTM1, GSTP1, and GSTO1 enzymes increase the risk of developing breast cancer and hepatocellular carcin...
Introduction. NOTCH pathway and TP53 protein are involved in the development of fibrosis and auto... more Introduction. NOTCH pathway and TP53 protein are involved in the development of fibrosis and autoimmune disorders, respectively. The aim of this study was to evaluate the role of single nucleotide polymorphisms (SNPs) of NOTCH3 and TP53 genes and serum anti-TP53 antibodies with the susceptibility, clinical subset of systemic sclerosis (SSc), and clinical profile of SSc patient, particularly with lung involvement and disease activity. Objects and Methods. 124 white Polish SSc patients (101 with limited cutaneous SSc–lcSSc, and 23 with diffuse cutaneous SSc–dcSSc) and 100 healthy individuals were included in the study. Patients were assessed for the presence of autoantibodies and interstitial lung disease. Two SNPs at position 6746 of NOTCH3 gene (C/T alleles) and 215 of the TP53 gene (P/R alleles) were genotyped by PCR-restriction fragment length polymorphism. Serum levels of anti-TP53 antibodies were analyzed by means of ELISA. Results. The genotypic frequencies of the NOTCH3 gene f...
Objectives: MicroRNAs have been observed to play a major role in various physiological processes,... more Objectives: MicroRNAs have been observed to play a major role in various physiological processes, for instance, programmed cell death, cell division, pregnancy development, and proliferation. With the help of profiling of microRNAs in the serum of pregnant women, it is possible to link alterations in their concentration to the emergence of gestational problems. The aim of the study was to evaluate the diagnostic potential of MicroRNAs Mi 517 and Mi 526 as biomarkers in the detection of hypertension and preeclampsia. Material and methods: The study considered 53 patients who are in their first trimester of a singleton pregnancy. Participants have been divided into two study groups, one group with normal pregnancy and another group having the risk of developing preeclampsia or who developed hypertension or preeclampsia during follow-up constitute the study group. In order to collect data associated with circulating miRNAs in serum, blood samples have been collected from the participants of the study. Results: Based on the univariate regression model, increased expression of Mi 517 and 526 and parity status (primapara/multipara) has been obtained. The multivariate logistic analysis shows that independent risk factors for hypertension or preeclampsia are the presence of an R527 and being a primipara. Conclusions: The study's findings have revealed that R517s and R526s act as major indicative biomarkers in the first trimester for the detection of hypertension and preeclampsia. The circulating C19MC MicroRNA was examined as a potential early indicator of preeclampsia and hypertension in pregnant individuals.
This study aimed to evaluate the safety and potential use of soy isoflavones in the treatment of ... more This study aimed to evaluate the safety and potential use of soy isoflavones in the treatment of skin problems, difficult-to-heal wounds and postoperative scars in women after the oncological treatment of breast cancer. The effects of different concentrations of genistein as a representative of soy isoflavonoids on MCF-7 tumor cells and BJ skin fibroblasts cultured in vitro were assessed. Genistein affects both healthy dermal BJ fibroblasts and cancerous MCF-7 cells. The effect of the tested isoflavonoid is closely related to its concentration. High concentrations of genistein destroy MCF-7 cancer cells, regardless of the exposure time, with a much greater effect on reducing cancer cell numbers at longer times (48 h). Lower concentrations of genistein (10 and 20 μM) increase the abundance of dermal fibroblasts. However, higher concentrations of genistein (50 μM and higher) are detrimental to fibroblasts at longer exposure times (48 h). Our studies indicate that although genistein sh...
alterations in microrna (mirna/mirs) expression are associated with the occurrence and course of ... more alterations in microrna (mirna/mirs) expression are associated with the occurrence and course of human diseases, including chronic lymphocytic leukemia (cll). expression of mirnas may vary among patients with cll in different cytogenetic risk groups. The present study assessed the expression levels of the following mirnas in 35 patients with cll: hsa-mir-15a,-16-1,-29a,-29c,-34a,-34b,-155,-181a,-181b,-221,-222 and-223. Fluorescent in situ hybridization (FiSH) analysis was performed for 13q14d, 17p13 and 11q22 deletions and chromosome 12 trisomy. Significantly higher expression levels of mir-181a,-221 and-223 were observed in the group at low risk of disease progression (stage 0) compared with the group with high risk of cll progression (P=0.036, P=0.019 and P=0.038, respectively). The present study revealed that the expression levels of mirna-181b and miRNA-223 were significantly higher in the group of patients without d13S319 deletion (P=0.039 and P=0.037, respectively). Moreover, the expression levels of mir-15a and mirna-29c were demonstrated to be significantly higher in the group of patients with cll who had a tumor protein p53 deletion, identified by FISH, compared with patients without this lesion (P=0.047, P=0.03 respectively). Based on receiver operating characteristic curve analysis, the present study revealed that mir-181a,-221 and-223 expression was able to distinguish low and high risk of cll progression in patients. among the tested mirnas, mirna-181a,-221 and-223 were indicated to have the greatest diagnostic potential in cll.
Despite significant medical advances, cancer treatment is still associated with a high risk of si... more Despite significant medical advances, cancer treatment is still associated with a high risk of side effects. The treatment is usually invasive and devastating and it affects the overall immunity of the whole organism, including the condition of the skin. In recent years there has been a growing interest in isoflavonoids, due to their wide range of biological and pharmacological activity, especially estrogen-like. It gives a broad perspective of their use as active ingredients of preparations, which eliminate skin lesions associated with oncological treatment. This article is an overview describing preclinical and clinical observations on the basis of available literature. It discusses the influence of genistein on skin health in women after breast cancer treatment. The overview focuses on studies conducted with genistein in vitro or in vivo to demonstrate its effect on skin, and anticancer properties. We selected articles from the last 20 years, available in the PubMed and Google Scholar databases.
Background: The aim of the current study is to analyze and summarize the latest research on impro... more Background: The aim of the current study is to analyze and summarize the latest research on improving therapy in ovarian cancer. Materials & methods: Data analysis was based on a review of publications from 2011 to 2021 in the PubMed database with use of the search terms including ‘EGFR ovarian cancer’, ‘folate receptor inhibitors ovarian cancer’, ‘VEGF ovarian cancer’, ‘PDGF ovarian cancer’ and ‘CTLA-4 ovarian cancer’. Results: 6643 articles were found; 238 clinical trials and randomized control trials were analyzed; 122 studies were rejected due to inconsistency with the topic of the work. Conclusion: Extensive research on the treatment of ovarian cancer increases the chance of developing the most effective therapy suited to the individual needs of the patient.
Oncology is one of the most rapidly developing fields in medicinal studies, however, it still can... more Oncology is one of the most rapidly developing fields in medicinal studies, however, it still cannot provide therapies that are efficient enough in neoplasms. As both morbidity and mortality of cancerous diseases increase annually, it is necessary to improve diagnostic and therapeutic procedures. One of the most promising areas is nanomedicine. The aim of the study is to review current therapeutic and diagnostic methods that involve nanotechnology. Methods Materials shared by the National Cancer Institute and research articles available in the PubMed database, that were searched by keywords "nanoparticle" and "cancer", were analyzed. Additionally, a search of clinical randomized trials was refined to a period over the last 5 years. Results: Over the last 5 years, studies on the use of nanoparticles in the treatment of breast cancer, thyroid cancer, pancreatic cancer, non-small cellular lung cancer, hepatocellular carcinoma, soft tissue sarcoma, cervical cancer, a...
Rak jajnika od lat jest przedmiotem szczególnego zainteresowania klinicystów i naukowców na całym... more Rak jajnika od lat jest przedmiotem szczególnego zainteresowania klinicystów i naukowców na całym świecie. Badania przeprowadzone w ostatnich latach w grupie pacjentek z rakiem jajnika wskazują na charakterystyczne podłoże konstytucyjne sprzyjające rozwojowi tego nowotworu. Na podstawie badań z zakresu genetyki wykazano, że zaburzenia w genach BRCA1, BRCA2 predysponują do rozwoju raka jajnika. Celem pracy była ocena mutacji genu BRCA1 u kobiety chorej na raka jajnika. W badaniu zastosowano metodę studium przypadku. Na podstawie oceny badania genetycznego stwierdzono, że obecność mutacji w genie BRCA1 była czynnikiem ryzyka wystąpienia nowotworu jajnika u badanej pacjentki. Edukacja kobiet w zakresie możliwości diagnostycznych i metod prewencji jest niezbędna dla uniknięcia raka jajnika lub zwiększenia szans na przeżycie w przypadku jego rozwoju.
Uploads
Papers by Agata A Filip