Papers by SAVERIO ManninO
This study was conducted to determine the impact of the HACCP management system on the safety of ... more This study was conducted to determine the impact of the HACCP management system on the safety of final products in baked goods production. The object of the study is two critical control points of the production of bakery products, which in turn affect the indicator of microbiological quality and safety of finished products. Using a mathematical model, the critical limits of critical control points (CCP) were determined – in the baking and cooling processes of baked goods. For the two CCPs, measures for continuous monitoring have been defined and critical limits have been developed. For CCT 1, a theoretical calculation using a mathematical model is given: the critical limit of the baking process (95.2–99.1 °C in the center of the crumb), by controlling this process, the company controls the biohazard factor, for CCP 2 the critical limit of cooling the finished product to a temperature of 22.84 °C in the center of the crumb for safe packing. The presentation of the results of the dev...
Journal of Dairy Science, 2000
We describe a new and rapid method for the evaluation of reactive sulfhydryl groups in whey prote... more We describe a new and rapid method for the evaluation of reactive sulfhydryl groups in whey proteins obtained after precipitation of casein by acetic acid at pH 4.6. The procedure is based on the use of a wire tungsten electrode operating at -0.2 V versus saturated calomel electrode in flow injection analysis. The method was applied to raw milks and to commercial pasteurized and UHT milks. Results showed that the tungsten electrode constituted a robust amperometric sensor that could be used to differentiate milks that underwent different heat treatments. The decrease of thiol content in the whey proteins from samples was in agreement with the whey protein content found by HPLC. The procedure is suitable for on-line quality control of heat-treated milks.
Lebensmittel Wissenschaft Technologie, 1988
Apres ultracentrifugation les echantillons sont injectes directement dans la colonne. La methode ... more Apres ultracentrifugation les echantillons sont injectes directement dans la colonne. La methode ne necessite pas de traitement chimique prealable. La detection electrochimique permet de detecter jusqu'a 0,1 ppm. Cette technique est recommandee pour les analyses de routine a grande echelle
Electrospun nanofibers have been investigated as novel packaging materials due to their unique ph... more Electrospun nanofibers have been investigated as novel packaging materials due to their unique physicochemical properties. Functionality of nanofibers may be broadened by inclusion of lipophilic functional molecules (e.g. flavors, antimicrobial, bioactives) in nanofibers. The feasibility of electrospinning polymer solutions containing oil-in-water emulsions as potential carrier vehicles for lipophilic functional compounds was investigated. Corn oil (5 wt%) was mixed with 2-5 wt% of surfactant solution (SDS or Tween 20) and homogenized using a laboratory scale ultrasonicator to produce o/w emulsions. Emulsions were mixed with high molecular weight poly(vinyl alcohol) (HMW-PVA) at pH 4.5 to yield solutions containing 1-10wt% emulsion and 1-10wt% PVA. Particle diameter emulsion droplets was determined by light scattering. Solutions were then electrospun and deposited structures characterized by optical and electron scanning microscopy. Emulsion had mean droplet diameters of 150-200nm using SDS and 300-400nm using Tween 20. Emulsion droplets in PVA solutions aggregated within a narrow concentration ratio range of emulsion droplets to polymer with the critical concentration ratio being higher for emulsions suspended in LMW-PVA compared to HMW-PVA, characteristic for depletion-induced flocculation. Jet formation was induced from mixed emulsion-polymer solutions at operating voltages >16kV. With HMW-PVA, at cpolymer>5wt%, nanofibers were deposited on the grounded target plate while at cpolymer10\ub5m), possibly coalesced droplets. Results suggest that upon electrospinning emulsion droplets are ejected as part of the polymer jet expulsion producing droplet-containing fibers that could be of great interest as novel active packaging materials
The Analyst, 1989
An approach to the construction of amperometric biosensors based on the incorporation of an enzym... more An approach to the construction of amperometric biosensors based on the incorporation of an enzyme in silicone grease and using the grease to fill micropores on a graphite surface is described. The enzyme-grease electrode concept, illustrated with the enzyme tyrosinase, offers a very simple, rapid and inexpensive approach to the fabrication of enzyme electrodes. The tyrosinase electrode responds very rapidly to dynamic changes in the concentration of phenolic compounds. A response time (t95%) as low as 5 s has been determined. With flow injection, 120 samples per hour can be processed with a relative standard deviation of 2.4%. The electrode remains active for about 12 d. The detection limit for dopamine is 6 x 10(-6) M. This method of biosensor construction should be applicable to other enzyme-substrate systems.
Talanta, 2009
Electronic nose and SPME-GCMS were used to monitor the autoxidation in long chain polyunsaturated... more Electronic nose and SPME-GCMS were used to monitor the autoxidation in long chain polyunsaturated fatty acid (LCPUFA)-enriched lipid microparticles produced by spray congealing with ultrasonic nebulization, during storage at 20 • C up to 6 weeks with sufficient air supply and limited air supply. Conjugated dienes and peroxide value as well as secondary lipid oxidation products were analysed to follow the course of autoxidation. Principal Component Analysis evidenced that only MOS sensors but not MOS-FET sensors contributed to the discrimination of the samples and facilitated the ability of the electronic nose to distinguish the LCPUFA-enriched lipid microparticles into two groups according to the different oxidative status. The selected MOS sensor responses correlated well with quantitative dominating volatile compounds (propanal and hexanal) and with volatile compounds which have been associated with fishy and rancid off flavour (1-penten-3-one, 1-penten-3-ol, 2,4-heptadienal and 2,6-nonadienal). Bread mix supplemented with the LCPUFA-enriched microparticles was analysed as an example for a LCPUFA supplemented food. Data from the present study indicate that the electronic nose can be used as a sensitive tool to evaluate the lipid oxidative status of LCPUFA-enriched microparticles. In supplemented foods like bread mix, matrix-related changes, which occur in supplemented and non-supplemented samples, make a clear distinction more difficult.
Talanta, 2009
A novel optical assay for sugar determination based on the catalytic and biocatalytic growth of g... more A novel optical assay for sugar determination based on the catalytic and biocatalytic growth of gold nanoparticles (AuNPs) is presented. The reaction of carbohydrates with these Au salts in alkaline media generates AuNPs at room temperature (RT) without the need for Au seeds in the solution or fibrous mesh. The optical properties of the resulting AuNPs relates to the total reducing sugar content of the samples analyzed. The development of such inexpensive optical assay was evaluated qualitatively and quantitatively on food beverages and honey samples. Its application can be of help to control the glucose content of the diet or easily extended in a host of industrial, biomedical and clinical fields.
Journal of Agricultural and Food Chemistry, 2001
In this paper, a novel electrochemical method to evaluate the antioxidant power of lipophilic com... more In this paper, a novel electrochemical method to evaluate the antioxidant power of lipophilic compounds present in vegetables, such as carotenoids, chlorophylls, tocopherols, and capsaicin, is reported. The method is based on a flow injection system with an electrochemical detector equipped with a glassy carbon working electrode operating amperometrically at a potential of + 0.5 V (vs Ag/AgCl). The proposed method is selective for lipophilic compounds having antioxidant power. When applied to pure compounds, the order of antioxidant power resulted as follows: lycopene > β-carotene Results obtained on five vegetable and two fruit extracts were compared to those obtained by the 2,2′-azinobis(3-ethylbenz-thiazoline-6-sulfonic) acid (ABTS) radical cation decolorization assay, one of the most used methods to evaluate the total antioxidant capacity of foods. A good correlation between the two methods was found, except for spinach, because of the different antioxidant powers assigned by the two methods to chlorophylls. In conclusion, results suggest that the proposed electrochemical method can be successfully employed for the direct, rapid, and reliable monitoring of the antioxidant power of lipophilic food extracts.
Food Chemistry, 2006
Oxidation is one of the most important processes of food deterioration since it may affect food s... more Oxidation is one of the most important processes of food deterioration since it may affect food safety, colour, flavour and texture. Antioxidants may protect food quality by preventing oxidative deterioration. In recent years, the restriction in the use of synthetic antioxidants, such as ...
Electroanalysis, 2012
The paper describes a rapid and simple method for the evaluation of the antioxidant capacity in o... more The paper describes a rapid and simple method for the evaluation of the antioxidant capacity in oil in water nanoemulsion. This procedure does not require extensive sample treatment and, most important, does not use any organic solvent for dissolution of the fatty matrix. The nanoemulsions were directly injected in a flow injection (FI) system with an electrochemical detector equipped with a glassy carbon working electrode operating amperometrically at a potential of +0.8 V (vs. Ag/AgCl). Results obtained were compared with those obtained by the Oxygen Radical Absorbance Capacity (ORAC) assay.
Electroanalysis, 1992
This review article assesses the status of electroanalysis in the food industry. Modern trends in... more This review article assesses the status of electroanalysis in the food industry. Modern trends in electroanalysis, which offer unique possibilities for assays of foods and drinks, and for monitoring food production processes, are discussed. Particular attention is given to the utility of new biocatalytic sensors, stripping techniques, and electrochemical flow detectors in the food industry. More than 90 references are given and future prospects are discussed.
Electroanalysis, 1993
A chemically modified electrode (CME) prepared by mixing cobalt(I1, 111) oxide and carbon paste, ... more A chemically modified electrode (CME) prepared by mixing cobalt(I1, 111) oxide and carbon paste, greatly reduces the OVerVohdge for the oxidation o f various biologically important compounds, such as sugars, hydrogen peroxide, and cysteine. The new electrocatalytic surface facilitates the amperometric detection of these compounds in flowing streams. The modified electrode is very stable, can be easily renewed, and gives reproducible results. Application for the determination of hydrogen peroxide in milk is demonstrated.
Electroanalysis, 1991
ABSTRACT The characteristics and applicability of a Nafion-copper modified electrode for the dete... more ABSTRACT The characteristics and applicability of a Nafion-copper modified electrode for the determination of some sugars in food samples are described. Coating with a Nafion membrane and subsequent immersion in a copper solution provides a good barrier against many electroactive hydroxyacids commonly found in food samples. The Nafion modified electrode is stable, can be easily renewed, and shows reproducible results. The analytical utility of the polymer-coated electrode is demonstrated by application to wine and milk samples.
Electroanalysis, 2009
A robust electrochemical method to measure the total phenol content in food samples is presented.... more A robust electrochemical method to measure the total phenol content in food samples is presented. Under optimal condition, BDD electrode showed excellent performance to detect the oxidation of several phenols and does not present the drawback due to electrode fouling. The analytical method used to perform such measurement has been optimized and successfully applied in different food samples. The results obtained were compared with the standard Folin -Ciocalteau method.
Electroanalysis, 2006
A new multi‐flow‐through amperometric detectors design was employed as a sensors array system for... more A new multi‐flow‐through amperometric detectors design was employed as a sensors array system for the detection of flavor related phenols. Relevant parameters of the amperometric detection were examined and optimized. The multivariate analytical signal was processed with chemometric analysis for exploring and classifying the tea beverages. Multivariate regression was used to correlate the astringency value of several tea beverages, obtained by the UNI (Italian Organization for Standardization) sensory profile test, with the signals collected by this amperometric electronic tongue.
Electroanalysis, 1997
A new microsystem, integrating microdialysis sampling and glucose oxidase immobilized on a tungst... more A new microsystem, integrating microdialysis sampling and glucose oxidase immobilized on a tungsten wire inserted into the lumen of the microdialysis probe, has been developed. The microsystem, coupled with amperometric detection, has proved to be viable for glucose determination in food samples. The immobilization procedure, based on the formation of an insoluble net formed by the enzyme with a polyanhydride (in the presence of polyethyleneimine), yielded a very stable enzyme layer that maintained about 75% of its initial activity after one month of daily use. Complex sample matrices (milk, juices) were successfully analyzed with little or no sample pretreatment using the fully automated flow system.
Chemical Communications, 2007
An integrated nanobioelectronic system, exploiting the distinct properties of nanowires and carbo... more An integrated nanobioelectronic system, exploiting the distinct properties of nanowires and carbon-nanotubes, has been designed for triggering reversibly and on-demand bioelectrocatalytic transformations of alcohols.
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Papers by SAVERIO ManninO