Permanent vision loss resulting from relapsing ocular inflammation occurs frequently in patients ... more Permanent vision loss resulting from relapsing ocular inflammation occurs frequently in patients with Behçet's disease, despite intensive and chronic immunosuppressive therapy. The authors report the case of a 35-year-old man with chronic bilateral posterior uveitis refractory to corticosteroids and immunosuppressive agents (methotrexate and cyclosporine) with a beneficial effect from treatment with infliximab.
The objective of this work was to evaluate an analytical method for the simultaneous measurement ... more The objective of this work was to evaluate an analytical method for the simultaneous measurement of sulfathiazole, sulfamethazine, and sulfadimethoxine in milk, in the form of azoderivatives, through the high performance liquid chromatography (HPLC). The isolation and concentration of sulfas were carried out by solid phase extraction (C18) with methanol as eluent. After extraction, analytes were transformed in diazonium salts and coupled with resorcinol. Derivatives were quantified by HPLC (C8 column, λ = 430 nm, MeOH/KH2PO4 aq). Recoveries were obtainedbetween 65 and 78%.
Jornal Brasileiro De Patologia E Medicina Laboratorial, 2008
The aim of this study was to investigate the existence of correlation between the clinical and hi... more The aim of this study was to investigate the existence of correlation between the clinical and histopathological data of oral squamous cell carcinoma. A total of 90 cases of patients diagnosed with oral squamous cell carcinoma were selected from the slide archives of Instituto Maranhense de Oncologia Aldenora Bello (São Luís, MA, Brazil). Pearsons correlation test was applied and did not reveal a statistically significant correlation (r = 0.095; p = 0.375) between TNM clinical classification and histologic malignancy grading, which was defined by Wahi (1971) and published by the World Health Organization (WHO). There was a significant correlation between the histologic malignancy grading and the size of the primary tumor as a clinical parameter (r = 0.229; p = 0.03).
Nup98 and Nup96 are components of the nuclear transport machinery and are induced by interferons ... more Nup98 and Nup96 are components of the nuclear transport machinery and are induced by interferons (IFN). Nup98 is a constituent of an mRNA export pathway that is targeted by viruses and regulated by IFN. However, the role of Nup96 in IFN-related mechanisms has not been established. To investigate the function of Nup96 in vivo, we generated Nup96 +/2 mice that express low levels of Nup96, as Nup96 2/2 mice are lethal. The Nup96 +/2 mice presented selective alterations of the immune system, which resulted in downregulation and impaired IFN a-and g-mediated induction of MHC I and IFNg induction of MHC II, ICAM-1, and other proteins. Frequency of TCRab+ and CD4+ T cells, which depends on MHC function, is reduced in NUP96 +/2 mice. Upon immunization, Nup96 +/2 mice showed impaired antigen presentation and T cell proliferation. Nup96 +/2 cells and mice were highly susceptible to viral infection, demonstrating a role for Nup96 in innate and adaptive immunity.
A method for HPLC determination of sulfadimethoxyne in milk is presented. The analyte isolation a... more A method for HPLC determination of sulfadimethoxyne in milk is presented. The analyte isolation and concentration were performed by solid-phase extraction through a C-8 cartridge, pre-conditioned with hexane, methanol and water and eluted with MeOH. The recovery determination was done with a spiked solution of 20, 50 or 100 µg L-1. In this concentration range, the recovery was 83.2% with a RDS of 15.4%. For quantification, a Zorbax Eclipse XDB-C8 (4.6 mm x 150 mm, 5 µm), a mobile phase of MeCN: 0.01 mol L-1 KH2PO4 aq. (1:4), and a variable wavelength detector (275 nm) were used.
Background: mRNAs are highly versatile, non-toxic molecules that are easy to produce and store, w... more Background: mRNAs are highly versatile, non-toxic molecules that are easy to produce and store, which can allow transient protein expression in all cell types. The safety aspects of mRNA-based treatments in gene therapy make this molecule one of the most promising active components of therapeutic or prophylactic methods. The use of mRNA as strategy for the stimulation of the immune system has been used mainly in current strategies for the cancer treatment but until now no one tested this molecule as vaccine for infectious disease. Results: We produce messenger RNA of Hsp65 protein from Mycobacterium leprae and show that vaccination of mice with a single dose of 10 μg of naked mRNA-Hsp65 through intranasal route was able to induce protection against subsequent challenge with virulent strain of Mycobacterium tuberculosis. Moreover it was shown that this immunization was associated with specific production of IL-10 and TNF-alpha in spleen. In order to determine if antigen presenting cells (APCs) present in the lung are capable of capture the mRNA, labeled mRNA-Hsp65 was administered by intranasal route and lung APCs were analyzed by flow cytometry. These experiments showed that after 30 minutes until 8 hours the populations of CD11c + , CD11b + and CD19 + cells were able to capture the mRNA. We also demonstrated in vitro that mRNA-Hsp65 leads nitric oxide (NO) production through Toll-like receptor 7 (TLR7).
Polycystic ovary syndrome (PCOS) is one of the most common endocrine diseases in women of reprodu... more Polycystic ovary syndrome (PCOS) is one of the most common endocrine diseases in women of reproductive age. PCOS typically develops during adolescence and is a heterogeneous syndrome classically characterized by features of anovulation combined with signs of androgen excess (hirsutism, acne). Increasing obesity in adolescents probably exacerbates signs of PCOS, contributing to its earlier recognition. Recognizing the features of this syndrome can be very challenging in adolescence. Although adolescents' concerns are often cosmetic, if left untreated these girls are at risk for diabetes, metabolic syndrome, and infertility as they mature. Efforts should be made to diagnose and treat PCOS to minimize the development of symptoms and prevent the onset of cardiovascular and metabolic disturbances. (A. Oliveira). Endocrinol Nutr. 2010;57(7):328-336 diagnosticar y tratar el SOP a fin de minimizar el desarrollo de los síntomas y prevenir la aparición de problemas cardiovasculares y metabólicos. & 2009 SEEN. Publicado por Elsevier España, S.L. Todos los derechos reservados.
1.2 CVM in the context of eliciting consumers' preferences for differentiated ... 3.... more 1.2 CVM in the context of eliciting consumers' preferences for differentiated ... 3.1.1 The In market consumer choice questionnaire variant....................... 11 ... 3.1.2 Pure referendum style questionnaire variant ..................................... 11 ... 4.2 Beef consumption: quantity, type and local of ...
PURPOSE: to evaluate periodontal conditions and need for treatment supplied by Periodontal Screen... more PURPOSE: to evaluate periodontal conditions and need for treatment supplied by Periodontal Screening and Recording (PSR) in mothers, in order to clarify the relationship between periodontal disease and low birth weight premature newborns. METHODS: PSR was used in a sample of 40 mothers, divided into: test group, consisting of mothers of premature newborns with weight less than 2,500 g (n=20), and the control group, consisting of mothers of term newborns with a weight equal to or over 2,500 g (n=20). The collected data were analyzed by descriptive statistics, and the results of PSR were submitted to statistical analysis in order to verify differences in periodontal condition and need for treatment of the mothers, using the Kolmogorov-Smirnov test, with a significance level of 5%. RESULTS: the presence of periodontal pocket of 3.5 to 5.5 mm was the most common finding among the mothers of newborns with low weight (39.2% of the cases), while the presence of bleeding at probing and absence of a periodontal pocket were the most frequent findings among the mothers of newborns with normal weight (37.5% of the cases). There was a statistically significant difference between periodontal conditions of the mothers of the groups (p=0.0494), but in relation to the need for treatment, there was no significant difference between the studied groups (p>0.05). CONCLUSION: the mothers of preterm newborns with low weight presented worse periodontal conditions, suggesting that periodontal infection may be related to preterm low birth weight newborns.
Anjos são Mensageiro de Deus (1Rs 19.5-7). Os anjos são espíritos que servem a Deus e ajudam os s... more Anjos são Mensageiro de Deus (1Rs 19.5-7). Os anjos são espíritos que servem a Deus e ajudam os salvos (Hb 1.14). Foram criados santos, mas alguns se revoltaram contra Deus (Jd 6; 2Pe 2.4). Em algumas passagens bíblicas Deus e o Anjo do SENHOR (de Javé) são a mesma pessoa .
Permanent vision loss resulting from relapsing ocular inflammation occurs frequently in patients ... more Permanent vision loss resulting from relapsing ocular inflammation occurs frequently in patients with Behçet's disease, despite intensive and chronic immunosuppressive therapy. The authors report the case of a 35-year-old man with chronic bilateral posterior uveitis refractory to corticosteroids and immunosuppressive agents (methotrexate and cyclosporine) with a beneficial effect from treatment with infliximab.
The objective of this work was to evaluate an analytical method for the simultaneous measurement ... more The objective of this work was to evaluate an analytical method for the simultaneous measurement of sulfathiazole, sulfamethazine, and sulfadimethoxine in milk, in the form of azoderivatives, through the high performance liquid chromatography (HPLC). The isolation and concentration of sulfas were carried out by solid phase extraction (C18) with methanol as eluent. After extraction, analytes were transformed in diazonium salts and coupled with resorcinol. Derivatives were quantified by HPLC (C8 column, λ = 430 nm, MeOH/KH2PO4 aq). Recoveries were obtainedbetween 65 and 78%.
Jornal Brasileiro De Patologia E Medicina Laboratorial, 2008
The aim of this study was to investigate the existence of correlation between the clinical and hi... more The aim of this study was to investigate the existence of correlation between the clinical and histopathological data of oral squamous cell carcinoma. A total of 90 cases of patients diagnosed with oral squamous cell carcinoma were selected from the slide archives of Instituto Maranhense de Oncologia Aldenora Bello (São Luís, MA, Brazil). Pearsons correlation test was applied and did not reveal a statistically significant correlation (r = 0.095; p = 0.375) between TNM clinical classification and histologic malignancy grading, which was defined by Wahi (1971) and published by the World Health Organization (WHO). There was a significant correlation between the histologic malignancy grading and the size of the primary tumor as a clinical parameter (r = 0.229; p = 0.03).
Nup98 and Nup96 are components of the nuclear transport machinery and are induced by interferons ... more Nup98 and Nup96 are components of the nuclear transport machinery and are induced by interferons (IFN). Nup98 is a constituent of an mRNA export pathway that is targeted by viruses and regulated by IFN. However, the role of Nup96 in IFN-related mechanisms has not been established. To investigate the function of Nup96 in vivo, we generated Nup96 +/2 mice that express low levels of Nup96, as Nup96 2/2 mice are lethal. The Nup96 +/2 mice presented selective alterations of the immune system, which resulted in downregulation and impaired IFN a-and g-mediated induction of MHC I and IFNg induction of MHC II, ICAM-1, and other proteins. Frequency of TCRab+ and CD4+ T cells, which depends on MHC function, is reduced in NUP96 +/2 mice. Upon immunization, Nup96 +/2 mice showed impaired antigen presentation and T cell proliferation. Nup96 +/2 cells and mice were highly susceptible to viral infection, demonstrating a role for Nup96 in innate and adaptive immunity.
A method for HPLC determination of sulfadimethoxyne in milk is presented. The analyte isolation a... more A method for HPLC determination of sulfadimethoxyne in milk is presented. The analyte isolation and concentration were performed by solid-phase extraction through a C-8 cartridge, pre-conditioned with hexane, methanol and water and eluted with MeOH. The recovery determination was done with a spiked solution of 20, 50 or 100 µg L-1. In this concentration range, the recovery was 83.2% with a RDS of 15.4%. For quantification, a Zorbax Eclipse XDB-C8 (4.6 mm x 150 mm, 5 µm), a mobile phase of MeCN: 0.01 mol L-1 KH2PO4 aq. (1:4), and a variable wavelength detector (275 nm) were used.
Background: mRNAs are highly versatile, non-toxic molecules that are easy to produce and store, w... more Background: mRNAs are highly versatile, non-toxic molecules that are easy to produce and store, which can allow transient protein expression in all cell types. The safety aspects of mRNA-based treatments in gene therapy make this molecule one of the most promising active components of therapeutic or prophylactic methods. The use of mRNA as strategy for the stimulation of the immune system has been used mainly in current strategies for the cancer treatment but until now no one tested this molecule as vaccine for infectious disease. Results: We produce messenger RNA of Hsp65 protein from Mycobacterium leprae and show that vaccination of mice with a single dose of 10 μg of naked mRNA-Hsp65 through intranasal route was able to induce protection against subsequent challenge with virulent strain of Mycobacterium tuberculosis. Moreover it was shown that this immunization was associated with specific production of IL-10 and TNF-alpha in spleen. In order to determine if antigen presenting cells (APCs) present in the lung are capable of capture the mRNA, labeled mRNA-Hsp65 was administered by intranasal route and lung APCs were analyzed by flow cytometry. These experiments showed that after 30 minutes until 8 hours the populations of CD11c + , CD11b + and CD19 + cells were able to capture the mRNA. We also demonstrated in vitro that mRNA-Hsp65 leads nitric oxide (NO) production through Toll-like receptor 7 (TLR7).
Polycystic ovary syndrome (PCOS) is one of the most common endocrine diseases in women of reprodu... more Polycystic ovary syndrome (PCOS) is one of the most common endocrine diseases in women of reproductive age. PCOS typically develops during adolescence and is a heterogeneous syndrome classically characterized by features of anovulation combined with signs of androgen excess (hirsutism, acne). Increasing obesity in adolescents probably exacerbates signs of PCOS, contributing to its earlier recognition. Recognizing the features of this syndrome can be very challenging in adolescence. Although adolescents' concerns are often cosmetic, if left untreated these girls are at risk for diabetes, metabolic syndrome, and infertility as they mature. Efforts should be made to diagnose and treat PCOS to minimize the development of symptoms and prevent the onset of cardiovascular and metabolic disturbances. (A. Oliveira). Endocrinol Nutr. 2010;57(7):328-336 diagnosticar y tratar el SOP a fin de minimizar el desarrollo de los síntomas y prevenir la aparición de problemas cardiovasculares y metabólicos. & 2009 SEEN. Publicado por Elsevier España, S.L. Todos los derechos reservados.
1.2 CVM in the context of eliciting consumers' preferences for differentiated ... 3.... more 1.2 CVM in the context of eliciting consumers' preferences for differentiated ... 3.1.1 The In market consumer choice questionnaire variant....................... 11 ... 3.1.2 Pure referendum style questionnaire variant ..................................... 11 ... 4.2 Beef consumption: quantity, type and local of ...
PURPOSE: to evaluate periodontal conditions and need for treatment supplied by Periodontal Screen... more PURPOSE: to evaluate periodontal conditions and need for treatment supplied by Periodontal Screening and Recording (PSR) in mothers, in order to clarify the relationship between periodontal disease and low birth weight premature newborns. METHODS: PSR was used in a sample of 40 mothers, divided into: test group, consisting of mothers of premature newborns with weight less than 2,500 g (n=20), and the control group, consisting of mothers of term newborns with a weight equal to or over 2,500 g (n=20). The collected data were analyzed by descriptive statistics, and the results of PSR were submitted to statistical analysis in order to verify differences in periodontal condition and need for treatment of the mothers, using the Kolmogorov-Smirnov test, with a significance level of 5%. RESULTS: the presence of periodontal pocket of 3.5 to 5.5 mm was the most common finding among the mothers of newborns with low weight (39.2% of the cases), while the presence of bleeding at probing and absence of a periodontal pocket were the most frequent findings among the mothers of newborns with normal weight (37.5% of the cases). There was a statistically significant difference between periodontal conditions of the mothers of the groups (p=0.0494), but in relation to the need for treatment, there was no significant difference between the studied groups (p>0.05). CONCLUSION: the mothers of preterm newborns with low weight presented worse periodontal conditions, suggesting that periodontal infection may be related to preterm low birth weight newborns.
Anjos são Mensageiro de Deus (1Rs 19.5-7). Os anjos são espíritos que servem a Deus e ajudam os s... more Anjos são Mensageiro de Deus (1Rs 19.5-7). Os anjos são espíritos que servem a Deus e ajudam os salvos (Hb 1.14). Foram criados santos, mas alguns se revoltaram contra Deus (Jd 6; 2Pe 2.4). Em algumas passagens bíblicas Deus e o Anjo do SENHOR (de Javé) são a mesma pessoa .
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