Objective: To determine the existence of cross-reactions between Leishmania serology and mycobact... more Objective: To determine the existence of cross-reactions between Leishmania serology and mycobacteriosis in patients infected with HIV-1.Patients and methods: HIV-positive individuals, with culture-proven episodes of tuberculosis or disseminated Mycobacterium avium complex (MAC) infection, were identified from the data files of our microbiology laboratory. Patients were included if leishmania serology had been performed within a period of 2 months before and 1 month after the diagnosis of mycobacterial disease.Results: We identified 106 cases of tuberculosis and 38 of disseminated MAC infection with concomitant leishmania serology in HIV-infected individuals. Among them, only two cases (1.8%), both with tuberculosis and without visceral leishmaniasis, showed positive leishmania serology. The two cases are discussed.Conclusions: We conclude that, in spite of its low sensitivity, serology is a useful diagnostic tool in co-infected patients, mainly because of its high specificity and the low rate of cross-reactivity with two of the most frequent causes of fever of unknown origin in HIV-positive patients from our area.
Diagnostic Microbiology and Infectious Disease, 2000
This study aims to evaluate the performance of a new vial (Myco/F Lytic) for the detection of myc... more This study aims to evaluate the performance of a new vial (Myco/F Lytic) for the detection of mycobacteria from blood specimens. This vial is monitored in the BACTEC 9000 blood culture system. We compared it with the traditional method routinely used in our laboratory, which is a lysis-centrifugation based procedure. Of 275 samples tested in parallel by both methods, 23 from 20 patients grew mycobacteria (18 Mycobacterium avium complex, 4 M. tuberculosis and 1 M. simiae); 11 isolates were recovered using both systems, 12 were isolated with the Myco/F Lytic medium only, and none were isolated using the traditional method only (p Ͻ 0.05). Blood was the diagnostic sample for 12 patients with the Myco/F Lytic system and only 7 with the traditional system. The mean time to detection of mycobacteria with Myco/F Lytic medium was 17 days, whereas it was 44 days with the traditional method (p Ͻ 0.001). Identification by DNA probes was performed directly from the Myco/F Lytic bottle. Myco/F Lytic is a rapid, simple, safe and highly reliable diagnostic method for the detection of mycobacteria in blood.
Objective: To determine the existence of cross-reactions between Leishmania serology and mycobact... more Objective: To determine the existence of cross-reactions between Leishmania serology and mycobacteriosis in patients infected with HIV-1.Patients and methods: HIV-positive individuals, with culture-proven episodes of tuberculosis or disseminated Mycobacterium avium complex (MAC) infection, were identified from the data files of our microbiology laboratory. Patients were included if leishmania serology had been performed within a period of 2 months before and 1 month after the diagnosis of mycobacterial disease.Results: We identified 106 cases of tuberculosis and 38 of disseminated MAC infection with concomitant leishmania serology in HIV-infected individuals. Among them, only two cases (1.8%), both with tuberculosis and without visceral leishmaniasis, showed positive leishmania serology. The two cases are discussed.Conclusions: We conclude that, in spite of its low sensitivity, serology is a useful diagnostic tool in co-infected patients, mainly because of its high specificity and the low rate of cross-reactivity with two of the most frequent causes of fever of unknown origin in HIV-positive patients from our area.
Diagnostic Microbiology and Infectious Disease, 2000
This study aims to evaluate the performance of a new vial (Myco/F Lytic) for the detection of myc... more This study aims to evaluate the performance of a new vial (Myco/F Lytic) for the detection of mycobacteria from blood specimens. This vial is monitored in the BACTEC 9000 blood culture system. We compared it with the traditional method routinely used in our laboratory, which is a lysis-centrifugation based procedure. Of 275 samples tested in parallel by both methods, 23 from 20 patients grew mycobacteria (18 Mycobacterium avium complex, 4 M. tuberculosis and 1 M. simiae); 11 isolates were recovered using both systems, 12 were isolated with the Myco/F Lytic medium only, and none were isolated using the traditional method only (p Ͻ 0.05). Blood was the diagnostic sample for 12 patients with the Myco/F Lytic system and only 7 with the traditional system. The mean time to detection of mycobacteria with Myco/F Lytic medium was 17 days, whereas it was 44 days with the traditional method (p Ͻ 0.001). Identification by DNA probes was performed directly from the Myco/F Lytic bottle. Myco/F Lytic is a rapid, simple, safe and highly reliable diagnostic method for the detection of mycobacteria in blood.
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