Papers by faith oviasogie
Archives of Applied Science Research, 2010
A total of 18 samples made up of six samples from hospital and 12 samples from municipal solid wa... more A total of 18 samples made up of six samples from hospital and 12 samples from municipal solid waste dump soil in Benin City were collected for isolation of viable aerobic bacteria. The following aerobic bacteria in decreasing order of prevalence were isolated from municipal solid waste, aerobic spore bearers (83.33%), Escherichia Coli (50.00%), Staphyloccus aureus (14.67%), Klebsiella sp (8.33%). Samples from the hospital waste yielded Pseudomonas aeruginosa, Klebsiella sp, Bacillus substilis with a prevalence of (60.00%) each, Serratia sp,Staphylococcus aureus and Escherichia Coli (20.00%) each. Antibiotic susceptibility tests on isolates were performed using standard disc diffusion method. Overall, Ciprofloxacin was the most effective agent on the isolates followed by Augmentin and Gentamycin in hospital and municipal solid waste respectively.Almost all isolates were resistant to Cloxacillin and Amoxiallin.
Preventive Nutrition and Food Science, 2020
The seeds of Citrus sinensis (L.) Osbeck (sweet orange) are waste products usually discarded. The... more The seeds of Citrus sinensis (L.) Osbeck (sweet orange) are waste products usually discarded. They may however contain phytochemicals that have potent bioactivities. In this study, the phenolic content, and antioxidant and antimicrobial activities of oil and non-oil (solid) extracts of C. sinensis seeds were evaluated using standard protocols. The seed oil contained significantly (P>0.05) higher contents of total phenol and total flavonoid when compared to the solid extract. However, the non-oil extract contained significantly (P<0.05) higher tannin contents than the seed oil. Ferric reducing antioxidant potential was not significantly different between both extracts. The antimicrobial activities of both extracts revealed that the seed oil possesses better antibacterial activities compared to the non-oil extract. The antifungal test revealed that the seed oil significantly inhibited the growth of Candida albicans (20 mm zone of inhibition at a concentration of 200 g/mL), however, it did not inhibit the growth of Aspergillus niger and Penicillum sp. The minimum inhibitory concentration values against the bacterial and fungal strains were similar for both extracts in the range of 50∼100 g/mL. Minimum bactericidal concentration and minimum fungicidal concentration values ranged from 100∼200 g/mL for both extracts. The results in this study indicate that C. sinensis seed oil and non-oil extracts possess antioxidant, and antibacterial and antifungal properties that may be differentially exploited in the development of antimicrobial agents.
International Journal of Biological and Chemical Sciences, 2018
Cedecea species are recent members of the family Enterobacteriaceae and are rarely incriminated i... more Cedecea species are recent members of the family Enterobacteriaceae and are rarely incriminated in human infections. A patient with protracted urinary tract infection, post-prostatectomy, was referred to Lahor Research Laboratories, Benin City, Nigeria for routine culture and susceptibility testing. Three consecutive mid-stream urine samples from him yielded pure, heavy, growths of an apparently same Gram negative, nonspore forming, non-capsulated, motile, rod-shaped bacterium, which was lipase, citrate, methyl red, KCN, ONPG, arginine and ornithine decarboxylase positive. It was oxidase, urease, H 2 S, indole, gelatinase, deoxyribonuclease and phenylalanine deaminase negative. It fermented glucose, xylose, sucrose, salicin, maltose mannitol, and lactose but was negative arabinose, inositol, dulcitol, raffinose, rhamnose and melibiose. It exhibited multiple antibiotic resistances to augmentin, gentamicin, ofloxacin, cefixime, ciprofloxacin, ceftazidime and cefuroxime but was very sensitive to imipenem and moderately susceptible to nitrofurantoin. The isolate was identified phylogenetically as Cedecea davisae. It possessed plasmid at 48.5 kb and harboured SHV, TEM, and gyrA antibiotic-resistance genes, which were either plasmid-mediated, chromosomal-mediated or both. To the best of knowledge, this is the first report on the incrimination of this organism with human infection in Nigeria. It was concluded that the paucity of reports on Cedecea species as aetiologic agents of human infections could be as a result of the difficulty in recognizing and characterizing this recently described pathogen, particularly in low resource laboratories, coupled with the fact that Laboratorians and Physicians are yet to be fully aware of the emerging clinical significance of this "relatively new" organism.
International Journal of Environmental Research and Public Health, 2016
The present study was designed to characterize methicillin-resistant staphylococci from raw meat.... more The present study was designed to characterize methicillin-resistant staphylococci from raw meat. A total of 126 meat samples were obtained from open markets between February and April, 2015. Antimicrobial susceptibility testing was carried out using the disc diffusion method. Molecular profiling was conducted using 16S rRNA, mecA, nuc, and PVL gene signatures were detected by polymerase chain reaction assay. Fifty isolates of methicillin-resistant Staphylococcus spp. were detected in 26 (52%) pork, 14 (28%) beef and 10 (20%) chicken samples. The staphylococcal isolates were identified through partial 16S ribosomal ribonucleic acid (16S rRNA) nucleotide sequencing, and BLAST analysis of the gene sequence revealed 98%-100% staphylococcal similarity. All isolates from beef and chicken samples amplified the mecA gene, while 100% of the MRSA isolates amplified the PVL gene. The multidrug resistance profile (resistant to ≥1 antimicrobial agent in ≥3 classes of antimicrobial agents) of the staphylococcal isolates showed that 7 isolates were resistant to methicillin, penicillin, clindamycin, chloramphenicol, trimethoprim-sulfamethoxazole, kanamycin, amoxicillin, cloxacillin, erythromycin, vancomycin, and gentamycin. There was a significant regression effect from the multidrug-resistant profile on the number of isolates (p < 0.05) suggesting a consequence of the dissemination of resistant strains within bacterial populations. The findings of the present study indicate that raw meats in the Benin metropolis were possibly contaminated with pathogenic and multi-drug resistant staphylococci strains and therefore could constitute a risk to public health communities.
Food Science & Nutrition, 2015
Journal of Diabetes Mellitus, 2013
C reactive protein is sensitive physiological biomarkers of sub clinical inflammation associated ... more C reactive protein is sensitive physiological biomarkers of sub clinical inflammation associated with hyperglycemia. The aim of this study is to determine the fasting serum C reactive protein level in type 2 diabetes mellitus patients attending diabetic clinic in Benin City, Nigeria. The population sample consists of 142 subjects. 71 patients were known type 2 diabetes mellitus, while the other 71 were age matched control subjects. Fasting glucose and C reactive protein were estimated using glucose oxidase method and ELISA method respectively. The age group that has the highest number of type 2 diabetes mellitus was 41-50 (64% of males and 36% of the females). Our finding revealed that C reactive protein and serum glucose level of type 2 Diabetes mellitus in both females and males show a statistically significant increase as compared with age matched Control subjects, (P < 0.05). An elevation of serum C-reactive protein was demonstrated in both males and females type 2 diabetes mellitus in Benin City, Nigeria. These data support a possible role of inflammatory biomarkers in diabetogenesis.
Open Journal of Immunology, 2013
Children especially in rural areas of Okada have high rates of intestinal parasitosis with a prev... more Children especially in rural areas of Okada have high rates of intestinal parasitosis with a prevalence of 50%. Stool and blood sample were collected of 334 children which comprised of 152 females and 185 males. Intestinal parasitosis was confirmed by direct smear technique and formol-ether concentration method. Serum IgE level was estimated by ELISA method. It was revealed that children between the ages of 11-15 years had the highest incidence of intestinal parasite in both sex (73.3% of the males and 62.5% of the females). About 2-fold elevation in serum IgE level was demonstrated. Intervention program including early introduction of health education to children is advocated and environmental sanitation should be encouraged.
African Journal of Microbiology Research, 2015
Citrus sinensis also known as sweet orange is the most popular of the citrus fruits. It is widely... more Citrus sinensis also known as sweet orange is the most popular of the citrus fruits. It is widely cultivated in most regions of the world possessing a rich source of vitamin C, flavonoids, phenolic compounds and pectin. This research was conducted to investigate the assessments of fungal pathogens associated with orange fruit spoilage sold in five markets in Benin metropolis and the possible public health implications. Some pathogenic fungal species were isolated from all five markets used in this study. Aspergillus species had the highest frequency and distribution from all sampling points (80%). Alternaria and Saccharomyces cerevisiae had the least occurrence from all sampling points (40% apiece). Candida, Mucor, Penicillium and Rhizopus had 60% occurrences, respectively. Candida tropicalis and species of Rhizopus, Penicillium, Aspergillus, Alternaria, and Mucor produced same symptoms and signs as observed in the original spoilt orange fruits before isolation. All fungal isolates were able to re-infect the healthy orange fruits with the exception of Alternaria species and Saccharomyces cerevisiae which were not able to grow and produce spoilage condition on the inoculated healthy orange fruits after five days. Aspergillus spp. are known to produce several toxic metabolites, like aflatoxins and ochratoxins, which are very important toxins worldwide because of the hazard it poses to human and animal health.
Tropical Medicine and Health, 2009
The epidemiology survey of Pfcrt and Pfmdr 1 mutant genes in malaria infection in Edo State was c... more The epidemiology survey of Pfcrt and Pfmdr 1 mutant genes in malaria infection in Edo State was carried out between June 2005 and May 2006. Five hundred and sixty one (561) subjects with a history of fever, joint pains, and bitterness of the mouth were enlisted for this study. With regard to place of residence, 229 subjects were from Edo South, 177 from Edo Central and 155 from Edo North. Genotyping of resistance markers "Plasmodium falciparum Chloroquine Resistance Transporter" (Pfcrt K76T) and Plasmodium falciparum Multi-Drug resistance (Pfmdr1) was carried out by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). There was no statistical difference (P>0.05) in the prevalence of malaria infection among the three senatorial districts (90.8%, 94.2% and 96.1% respectively). The general prevalence of mutant Pfcrt K76T and Pfmdr 1 mutant genes in the state were 21.9% and 15.1% respectively. No statistical difference existed when the prevalence of these genes was compared within the senatorial districts (P>0.05). The treatment of malaria has reached a crisis point in the sub-Sahara regions. The present findings also revealed a relatively high prevalence of these resistance genes in Edo State, underlining the need for urgent policy intervention before the situation escalates beyond control.
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Papers by faith oviasogie