Molecular Pathology Catalog 2014 - 2015

Molecular Pathology Catalog 2014 - 2015 (International) Dear Customer, We are pleased to present the BioGenex Molecular Pathology Catalog for 2014 - 2015. As a vertically integrated company we develop, manufacture and market highly innovative and fully automated systems for cancer diagnosis, prognosis and therapy selection. Xmatrx® systems redefine complete automation for the molecular pathology laboratory and standardize all the steps from baking through final cover-slipping in three simple steps - Load, Click and View. Compared to any other system on the market, Xmatrx® systems offer clean intense stain(s), automate more assay steps, and enable automation of technologies for the future molecular pathology laboratory. • Xmatrx® ELITE integrates All-in-One and All-at-Once staining of IHC, ISH, special stains and beyond • Xmatrx® Infinity is a high-performance staining platform for life sciences and translational research • Xmatrx® ULTRA is the next-generation system with new features such as touch screen and SMS intelligence • Xmatrx® NANO is a ten-slide automated system specifically designed for FISH • Xmatrx® MINI enables in situ PCR and nucleic acid hybridization with tools for building micro-chamber We also offer a series of i6000TM systems with very high throughput; 200 slides in an 8-hour shift. To maintain our tradition of offering superior solutions for the emerging needs of your laboratory, we offer a broad range of molecular pathology products for IHC, ISH, miRNA, multiplex and special staining of tissues including 300+ primary antibodies, molecular probes, detection systems, and ancillaries. These are offered for standardized, reliable and consistent results to support the needs of molecular pathology laboratories of today, tomorrow and beyond. BioGenex is committed to the core values of innovation, reliability, productivity, quality and superior after sales support and service for complete customer satisfaction. These values are represented by our company’s colors that stand for “energy and innovation” (orange) and “reliability” (blue). I invite you to learn more about our exciting products and future development through this catalog and our new website at www.biogenex.com. Should you have any suggestions for improving our products and services, I encourage you to write me directly at k.kalra@biogenex.com. Give us an opportunity and experience the difference. Warm Regards, Krishan Kalra, Ph.D. CEO internationalcs@biogenex.com i “ To become a global molecular medicine company providing affordable solutions “ for life science research and personalized medicine Dr. Krishan Kalra Innovation Quality Service Reliability Productivity ii www.biogenex.com Table of Contents Overview.....................................................................................iv Multi-Staining General Information..................................................................v Super Sensitive™ Double Stain Polymer Detection System.......144 Additional Information .............................................................v Super Sensitive™ Antibody Cocktails......................................145 Ordering Information.................................................................vi Technical Information...............................................................vi ISH Probes & ISH Detection Systems in situ Hybridization Probes.....................................................150 Automated Systems microRNA Probes......................................................................152 Xmatrx® ELITE...........................................................................02 Hybridization Detection System...............................................158 ® Xmatrx ULTRA.........................................................................03 Xmatrx® NANO..........................................................................04 eFISHiency Xmatrx® MINI............................................................................05 eFISHiency................................................................................160 Xmatrx® Infinity.........................................................................06 eFISHiency probes....................................................................161 ® Xmatrx FISH (eFISHiency)......................................................07 eFISH pretreatment kits...........................................................163 i6000TM Diagnostics................................................................08 eFISH Processing systems.......................................................164 i6000TM Infinity........................................................................09 i500 PlusTM...............................................................................10 ® Xmatrx Associated Reagents and Consumables.................11 Special Stains Special Stains...........................................................................168 i6000TM Associated Reagents and Consumables.................23 i500 PlusTM Associated Consumables....................................30 Ancillary Reagents Buffers and Diluents................................................................176 Tissue Pre-treatment & Antigen Retrieval Blocking Reagents....................................................................177 EZ-DeWaxTM Solution................................................................32 Substrates and Chromogens...................................................178 Antigen Retrieval Method........................................................33 Negative Control Sera/Immunoglobulins...............................179 Antigen Retrieval Accessory Kit..............................................34 Counterstains and Mounting Media.......................................180 TM EZ-AR Elegance Solutions....................................................34 Microscope Slides and Accessories........................................181 EZ-ARTM Solutions....................................................................35 Antigen Retrieval Solutions.....................................................36 Tissue Control Enzymes for Tissue Digestion.................................................38 Positive Control Slides and Barrier Slides..............................184 ® NordicWare Microwave Tender Cooker.................................39 Index Primary Antibodies Alphabetical Product Name Index .........................................194 Antibody Look-Up Table.......................................................... 43 Catalog Number Index.............................................................210 Antibody Details.......................................................................51 Antibody Clone Index...............................................................219 Listing by Categories...............................................................228 IHC Detection Systems Super SensitiveTM IHC Detection Systems...........................138 TM Super Sensitive General Terms and Conditions...............................................237 One-Step Polymer-HRP Detection- Systems.................................................................................139 Super SensitiveTM Link-Label IHC Detection Systems.........140 Links and Labels...................................................................142 For latest product offerings visit our website www.biogenex.com or contact our customer support: internationalcs@biogenex.com internationalcs@biogenex.com iii Overview BioGenex celebrated its 33rd anniversary serving the anatomic pathology market. We take great pride in providing premier service and support while bringing new and technologically advanced products to the market. Reliability BioGenex provides a “Total Solution” for slide-based cell and tissue analysis. Our products include a wide variety of antibodies, highly sensitive detection kits, and probes for ISH. Our automated systems streamline operations in molecular and cellular pathology laboratories, providing effective tools for the detection and diagnosis of cancer and other diseases. BioGenex continues to innovate as evidenced by the launch of the Xmatrx® Staining System which provides complete automation “From Microtome to Microscope”. Innovation We are committed to providing our customers and our distributors with flexible, innovative and cost-effective tools for clinical diagnostics, life science research and drug discovery. Service We value you and your business. We want our relationship to be one of total satisfaction. Our Technical Support Specialists provide fast troubleshooting advice and technical information and they are responsive to your individual needs. Just visit our website at www.biogenex.com, send an e-mail to support@biogenex.com or call toll free at 1-(800)-421-4149 from 7:00 AM to 4:00 PM (PST), Monday through Friday, with your request. BioGenex products give consistent, reproducible and reliable results. Our automated systems are highly reliable and dependable, giving our customer peace of mind. BioGenex has a rich history of innovation in the field of Immunohistochemistry (IHC) and In situ Hybridization (ISH). BioGenex has a strong intellectual portfolio, consisting of several US and foreign-issued patents, in the areas of • • • • • DNA labeling and amplification Antigen retrieval and deparaffinization Automation of tissue and cell sample preparation Automated IHC, and staining of nucleic Acids Antigen retrieval and nucleic acid retrieval for tissues Productivity BioGenex has automated cell and tissue analysis to accelerate clinical diagnostics and drug discovery development. We have developed the total walk-away, industrial scale automated systems to streamline and standardize an array of processes for cell and tissue testing in IHC, ISH/CISH, FISH, and image analysis applications. We offer a “Total Solution” automating every aspect of the histology slide preparation “From Microtome to Microscope”. These technologies significantly increase laboratory operation productivity for clinical diagnostics, drug discovery and life sciences research applications by providing high-quality staining and imaging solutions. Quality BioGenex is committed to excellence by providing high-quality products. We offer a broad range of products which are manufactured using state-of-the-art equipment in controlled environments. They are stringently tested to ensure that they meet or exceed functional, dimensional, and environmental requirements and are compliant with federal regulations. Our automated systems are designed for high-throughput at a low cost of ownership. They provide consistent quality results with ease of use and maximum flexibility for clinical diagnostics, life science research, and drug discovery markets. iv www.biogenex.com General Information Web Site Upg rade d Nationwide Training Workshops For the latest information on new product releases, special offers and for placing an online quote request, please visit our new website, www.biogenex.com Customer Support Our technical support and customer service specialists are ready to provide fast and detailed Information for your questions and needs. Please call our toll-free number to reach us. Technical Support Tel: Fax: E-mail: Website: 1-(800)-421-4149 (Option 2) 1-(510)-824-1490 support@biogenex.com www.biogenex.com Customer Service Tel: Fax: Fax: E-mail: Additional Information 1-(800)-421-4149 (Option 1) 1-(510)-824-1490 1-(888)-866-2500 (Orders Only) internationalcs@biogenex.com Corporate Office BioGenex Laboratories, Inc. 49026 Milmont Drive Fremont, CA 94538 Tel: 1-(510)-824-1400 Fax: 1-(510)-824-1490 Corporate Business For general business matters not related to product orders or inquiries, please call us at 1-(510) 824-1400 or fax your correspondence to our main corporate business fax: 1-(510) 824-1490. Trademarks The following are trademarks of BioGenex Laboratories, Inc. USA BioGenex® EZ-ARTM EZ-Retriever® MultiLink® Super SensitiveTM i6000TM EZ-DeWaxTM GenoMx® i500 PlusTM Xmatrx® Power BlockTM XMountTM OptiPlusTM XVizTM InSite® Super Mount® XWashTM XISHTM internationalcs@biogenex.com As a service to our customers, BioGenex has developed lectures and workshops on the full range of Immunohistochemistry and in situ Hybridization techniques. Please call our Technical Support Department or Regional Account Executive for more information on how you can participate in our educational workshops. Topics include the following: • Basic Immunohistochemistry • Cancer Panels • Microwave-Based Antigen Retrieval • ER/PR Immunostaining • Troubleshooting • Automation • in situ Hybridization • Double Staining • Multiplexing and Co-detection of Protein and Nucleic Acid Biomarkers Free Technical Literature In addition to the educational brochures produced by BioGenex, we offer other technically useful information to the histopathology specialists on our website, www.biogenex.com where you can download our data sheet, product catalog or relevant presentation that may accompany each product assay protocols, kit instruction manuals and conference posters. Please call our Technical support department to request specific items or to add your name to our mailing list. Technology Partnering Opportunities We are always interested in licensing innovative technology that will be useful to our customers. If you are a researcher and have new antibody clones or other new diagnostic technologies please think of BioGenex as a potential partner in marketing your inventions and discoveries. We have the scientific expertise and marketing experience necessary for the successful commercialization of your technical achievements. BioGenex has an active Research and Development program fully staffed with PhD and MD professionals who are experienced in immunopathology, protein chemistry, and molecular biology. For more information on technology transfer opportunities, please contact us at customer.service@biogenex.com v Ordering Information International Orders To place an order from outside the US, please contact your local BioGenex channel partner/distributor. Please visit our website www.biogenex.com, for more details. For countries where BioGenex does not have any channel partners/distributors, please e-mail us at internationalcs@biogenex.com Payment All payments must be made in U.S. dollars. The following methods of payment are accepted: • Bank transfer (see Proforma Invoice for instructions) • Check, drawn on a U.S. bank, made payable to: “BioGenex Laboratories, Inc.” Technical Information All BioGenex products have been listed in this catalog under easily identifiable product groups. The products have also been indexed at end of the catalog under the following headings: • • • • Alphabetical Product Name Index Catalog Number Index Antibody Clone Index Listing By Categories Symbol keys used in different sections have been defined on the same page for quick and easy reference. The BioGenex Molecular Pathology Catalog 2014 - 2015 is also available on our website, www.biogenex.com vi www.biogenex.com Automation Automated Systems Microtome to Microscope Fully Automated System from Microtome to Microscope... For the Molecular Pathology Laboratory of Today, Tomorrow and Beyond • All-in-One All-at-Once – IHC, ISH, SS, Multiplexing and Co-detection • FISH* • Standardized process from baking through inal glass cover slip in three simple steps: Load – Click – View slides • Automates technologies of today…IHC, ISH, SS, FISH*, Multiplexing and Co-detection of Nucleic Acid and Protein Biomarkers • Designed to automate the technologies of tomorrow and beyond…Gene Expression Proiling, in situ PCR, miRNA… and more • Meets global regulatory requirements and safety standards * optional software 2 www.biogenex.com Automated Systems Next Generation Fully Automated Staining System All-in-One All-at-Once - IHC, ISH, SS, Multiplex and Co-detection • Next generation fully-automated slide staining system • BioGenex’s proprietary coverslip mechanism • Easy waste disposal system • Intelligent SMS system • Auto-DAB enabled – On-board automated mixing of chromogen and buffer • High throughput - 100 slides per day, 60 slides in eight-hour shift, and 40 slides in delayed overnight run internationalcs@biogenex.com 3 Automated Systems eFISHiency System for FISH Automation All-in-One - FISH, in situ PCR and ISH • Add micro-reagents manually to save cost • Intelligent SMS information for alerts • Economical and affordable • Touch Panel PC as user interface • Flexible Open System Software - create, edit and save protocols for future use • Run 10 different protocols at the same time 4 www.biogenex.com Automated Systems eFISHiency Workstation All-in-One - FISH, in situ PCR and ISH • High performance in situ PCR and FISH • Hybridizer with eXACT™ temperatures • 10 independent thermal cyclers • Built-in touch screen display for easy operations • Facility of on-board wash with effective waste drainage system • Audio-visual alerts and on screen color-coded error alerts • User-friendly software with ability to add/delete cycles, store protocols for future use and perform up to 45 PCR cycles internationalcs@biogenex.com 5 Automated Systems Infinite Possibilities... ...For Translational and Clinical Research All-in-One - IHC, IF, ISH, CISH, FISH, in situ PCR and miRNA... • Intelligent and lexible system offering ininite possibilities – IHC, ISH, FISH, CISH, IF, Multiplexing and Co-detection • Simultaneous optimization of up to 40 parameters in single run • Reaction micro-chamber reduces micro-reagent consumption by up to 90% • 40 independent thermocyclable (PCR) workstations • Intuitive software designed for ease of use and lexibility • Reports for inventory management and regulatory compliance • Multiple slide processing options – Random, Continuous and STAT 6 www.biogenex.com Automated Systems Integrated Workflow Solutions for Optimizing Productivity Microtome to Microscope • The world’s irst and only fully automated front-end FISH processing system • Run up to 40 slides under multiple protocols • Reduce hands-on tech time from 7.5 hours to 30 minutes 33 Steps Reduced to 3 eFISHiency System for FISH Automation • On-board dewaxing, oil seal and inal coverslip after DAPI • Add micro-reagents manually to save cost • Run 10 different protocols at the same time • Intelligent SMS information for alerts 33 Steps Reduced to 6 eFISHiency Workstation • eFISHiency Workstation for manual FISH assay • Hybridizer with eXACTTM temperatures • 10 Independently programmable thermal cyclers • Built-in touch screen display • Manual coverslip application and removal Accessories Oil stamp Coverslip stand internationalcs@biogenex.com Suction pen 7 Automated Systems Walkaway IHC Staining System Multiplex IHC System • Clean, crisp and intense stains • High throughput – Up to 200 slides in eight-hour shift, 60 slides in 3 hours • Over 300+ optimized protocols with ready-to-use reagents in barcoded vials • Dispense reagents as low as 100µL/slide • Multiple slide processing options - Random, Continuous and STAT • Multi-format specimen processing - FFPE or frozen tissues, cell preparations, ine needle aspirates, smears and more… • Color-coded GUI with real-time assay parameter display for all slides • Customized or standard reports for inventory management and regulatory compliance and submission 8 www.biogenex.com Automated Systems Multifunctional Staining System for Research Multi-functional System - Multiplex IHC, ISH, IF and Special Stains • Fully open system to customize any manual protocol • Simultaneous optimization of up to 60 assay parameters • Disposable pipette tips – eliminates cross contamination • Audio and visual alerts at every step for manual intervention • Customized reporting system for detailed report generation • Multiple slide processing options – Random, Continuous and STAT internationalcs@biogenex.com 9 Automated Systems LIS Enabled Barcode Label Printer Integrated Digitized Data Tracking System • For printing chemical resistant barcode labels • Compatible with Xmatrx® and i6000TM • User-friendly software • Synchronization of protocol information • Efficient system • Eliminates human error • Helps reduce operating cost • Fast turn-around 10 www.biogenex.com Automated Systems Xmatrx Automated Staining System ® Product Name Cat. No. ® Xmatrx ELITE AS4040B Xmatrx® Infinity AS4000RX Xmatrx® NANO AS1000 ® Xmatrx MINI AS1010 Xmatrx® ULTRA AS4030B Reagents and Consumables Immunohistochemistry – Reagents Primary antibodies in RFID tagged vials for 200 slides Primary Antibodies Product Name Cat. No. Clone/Source Pre-treatment Aberrant Endothelial Cell AX382-YCD 4A11/Mouse HX031-YCDE ACTH AX487-YCD AH26/Mouse HX031-YCDE Actin Muscle-specific AX090-YCD HHF35/Mouse None Actin Smooth muscle AX128-YCD 1A4/Mouse None Adenovirus AX059-YCDE A62020069P/Mouse HX031-YCDE/HX032-YCDE Alpha 1 Antitrypsin AW015-YCD Polyclonal/Rabbit HX031-YCDE/HX032-YCDE Alpha Actinin AX097-YCD JLN20/Mouse HX031-YCDE/HX032-YCDE Alpha-1-Antichymotrypsin AX388-YCD α1A88/Mouse HX031-YCDE Alpha-Fetoprotein (AFP) AX008-YCD C3/Mouse None Androgen Receptor AX256-YCDE F39.4.1/Mouse HX032-YCDE Apolipoprotein E AX567-YCD EP1373Y/Rabbit HX031-YCDE/HX032-YCDE B Cell AX158-YCD MB2/Mouse HX031-YCDE B Lymphocyte antigen 36 (BLA-36) AX231-YCD A27-42/Mouse HX031-YCDE/HX032-YCDE Bax Protein AW347-YCD Polyclonal/Rabbit HX031-YCDE/HX032-YCDE Breast Cancer Antigen BCA-225 AX135-YCD CU18/Mouse HX031-YCDE Bcl 2 AX541-YCD E17/Rabbit HX031-YCDE Bcl 2 AY723-YCD EP36/Rabbit None Bcl-2 Oncoprotein AX287-YCD Bcl-2/100/Mouse HX031-YCDE Bcl-6 AY708-YCD LN22/Mouse None Bcl-x AX462-YCD E18/Rabbit HX031-YCDE Beta-Catenin AX510-YCD E247/Rabbit HX031-YCDE/HX032-YCDE bFGF (Basic growth Factor) AX359-YCD bFGF88/Mouse HX031-YCDE BOB-1 (New) AY731-YCDE SP92/Rabbit None BRCA1 Protein AW345-YCD Polyclonal/Rabbit HX031-YCDE/HX032-YCDE CA19-9 AX424-YCD C241:5:1:4/Mouse HX032-YCDE CA 125 (Ovarian Tumor Marker) AX429-YCD Ov185:1/Mouse HX031-YCDE/HX032-YCDE Caldesmon AX507-YCD E89/Rabbit HX031-YCDE Caldesmon, High MW, Smooth muscle AX332-YCD h-CD/Mouse HX031-YCDE Calponin AX333-YCD CALP/Mouse HX031-YCDE Calponin-1 (C-term) AX502-YCD EP798Y/Rabbit HX031-YCDE Calretinin AX583-YCD 2 E7/Mouse HX031-YCDE Calretinin AW413-YCD Polyclonal/Rabbit HX031-YCDE Carcinoembryonic Antigen (CEA) AX009-YCD B01-94-11M-P/Mouse None Carcinoembryonic Antigen (CEA) AW009-YCD Polyclonal/Rabbit HX031-YCDE Catenin (p120) AX523-YCD YE372/Rabbit HX031-YCDE/HX032-YCDE Catenin Delta 1 (p120) AW706-YCD Polyclonal/Rabbit HX031-YCDE Cathepsin D AX467-YCD C15/Mouse HX031-YCDE internationalcs@biogenex.com 11 Automated Systems Xmatrx® Reagents and Consumables Product Name Cat. No. Clone/Source Pre-treatment CD1a AX490-YCD O10/Mouse HX031-YCDE/HX032-YCDE CD2 AX438-YCD AB75/Mouse HX032-YCDE CD3 Epsilon AX477-YCD EP449E/Rabbit HX032-YCDE CD3 (T cell) AX322-YCD PS1/Mouse HX031-YCDE CD3 (T Cell) AX258-YCD UCHT1/Mouse HX031-YCDE CD3 + CD20 AC559-YCD EP449E/Rabbit/L-26/Mouse HX031-YCDE/HX032-YCDE CD4 AX421-YCD 4B12/Mouse HX031E-YCDE CD4 (New) AY722-YCD EP204/Rabbit HX032E-YCDE CD4 and CD8 Cocktail AC595-YCD 4B12+EP1150Y/Mouse+Rabbit HX031-YCDE/HX032-YCDE CD5 AX512-YCD EP2952/Rabbit HX031-YCDE/HX032-YCDE CD5 Monoclonal AX430-YCD 4C7/Mouse HX032-YCDE CD7 AY702-YCD LP15/Mouse HX031-YCDE CD8 AX422-YCD 1A5/Mouse HX032-YCDE CD8 AX513-YCD EP1150Y/Rabbit HX031-YCDE/HX032-YCDE CD8 (New) AY740-YCD SP16/Rabbit HX032-YCDE CD8 (T Cell, Suppressor/Cytotoxic) AX261-YCD T8/Mouse HX031-YCDE/HX032-YCDE CD10 AX451-YCD 56C6/Mouse HX031-YCDE/HX032-YCDE CD10 + Cyclin D1 AC561-YCD 56C6/Mouse/EPR2241/Rabbit HX031-YCDE/HX032-YCDE CD11b (C3bi Receptor) AX270-YCD M01/Mouse HX031-YCDE/HX032-YCDE CD11b/ITAM AX546-YCD EPR1344/Rabbit HX031-YCDE CD11c AX555-YCD EP1347Y/Rabbit HX031-YCDE/HX032-YCDE CD14 AX515-YCD EPR3653/Rabbit HX031-YCDE CD15 (Blood group antigen Lewis X) AX302-YCD BRA4F1/Mouse None CD16 AX437-YCD 2H7/Mouse HX032-YCDE CD19 (New) AY729-YCD EP169/Rabbit HX031-YCDE CD20 AX537-YCD CD20/C23/Mouse HX031-YCDE/HX032-YCDE CD20 (B cell) AX238-YCD L-26/Mouse None CD20 (B Cell) AX265-YCD B1/Mouse HX031-YCDE/HX032-YCDE CD21 AX497-YCD EP3093/Rabbit HX031-YCDE/HX032-YCDE CD21 (B Cell) AX266-YCD B2/Mouse HX031-YCDE/HX032-YCDE CD22 AX439-YCD FPC1/Mouse HX031-YCDE CD23 AW460-YCD Polyclonal/Rabbit HX031-YCDE CD25 AX453-YCD 4C9/Mouse HX031-YCDE/HX032-YCDE CD29 (Integrin beta-1 subunit) AX298-YCD JB1a/Mouse HX032-YCDE CD30 (Ki-1 antigen) AX327-YCD Ber-H2/Mouse HX031-YCDE CD30 (Ki-1 antigen) AX351-YCD HRS-4/Mouse HX031-YCDE CD31 (Endothelial cell) AX232-YCD JC/70A/Mouse HX032-YCDE CD31 (PECAM-1) AX241-YCD 9G11/Mouse HX031-YCDE/HX032-YCDE CD34 (Endothelial cell) AX236-YCD QBend/10/Mouse None CD35 AX431-YCD RLB25/Mouse HX031-YCDE/HX032-YCDE CD35 (New) AY741-YCD SP191/Rabbit None CD41/Integrin Alpha IIb (New) AY732-YCDE EP178/Rabbit None CD43 & CD45RA AX159-YCD MT1 & MB1/Mouse HX031-YCDE/HX032-YCDE CD43 (T cell Leukosialin) AX305-YCD DFT-1/Mouse HX031-YCDE CD44 (Phagocytic glycoprotein-1 HCAM) AX310-YCD DF1485/Mouse None CD45 AX111-YCD PD7/26/16&2B11/Mouse None CD45 AX338-YCD LJ27.9/Mouse HX031-YCDE/HX032-YCDE CD45 cocktail (Leukocyte common antigen LCA) AX371-YCD MEM55+LJ 24.9/Mouse None CD45RA (B cell) AX157-YCD MB1/Mouse None CD45RB AX320-YCD MEM55/Mouse HX031-YCDE/HX032-YCDE 12 www.biogenex.com Automated Systems Xmatrx® Reagents and Consumables Product Name Cat. No. Clone/Source Pre-treatment CD45RC (T cell) AX156-YCD MT2/Mouse None CD45RO (T cell) AX113-YCD UCHL-1/Mouse None CD48 (New) AY721-YCDE EP148/Rabbit None CD53 (New) AY734-YCD EP179/Rabbit None CD56 (Natural Killer cells, NCAM) AX268-YCD NKH-1/Mouse HX031-YCDE/HX032-YCDE CD57 (Natural killer cell) AX314-YCD NK-1/Mouse None CD61 AX482-YCD EP2417Y/Rabbit HX031-YCDE CD63 (New) AY720-YCDE EP211/Rabbit HK031-YCDE CD66 AX325-YCD BY114/Mouse None CD68 AX416-YCD KP1/Mouse HX031-YCDE CD68 AX549-YCD CD68/G2/Mouse HX031-YCDE CD71 (Transferrin Receptor) AX269-YCD T9/Mouse HX031-YCDE/HX032-YCDE CD71 (Transferrin Receptor) AX354-YCD H68.4/Mouse HX031-YCDE/HX032-YCDE CD74 (B Cell) AX153-YCD LN2/Mouse HX031-YCDE/HX032-YCDE CD79a AX414-YCD 11E3/Mouse HX032-YCDE CD79a (New) AY719-YCD EP82/Rabbit HK031-YCDE CD90 (New) AY733-YCD EP56/Rabbit None CD95 (New) AY742-YCD EP208/Rabbit None CD99 AX518-YCD EPR3097Y/Rabbit HX031-YCDE/HX032-YCDE CD99 (MIC2 antigen) AX355-YCD HO36.1.1/Mouse HX032-YCDE CD103 (New) AY739-YCDE EP206/Rabbit None CD105 AX441-YCD 4G11/Mouse HX031-YCDE/HX032-YCDE CD117 AX423-YCD T595/Mouse HX031-YCDE CD117 AX465-YCD YR145/Rabbit HX031-YCDE/HX032-YCDE CD146 (New) AY716-YCD EP54/Rabbit HX032-YCDE CD205 (New) AY737-YCDE EP176/Rabbit HX031-YCDE CD227 (Mucin 1) AX534-YCD YU-4H5/MOUSE HX031-YCDE CDw75 (B Cell) AX152-YCD LN1/Mouse HX031-YCDE/HX032-YCDE CDX-2 AX392-YCD CDX2-88/Mouse HX032-YCDE CDX-2, CK20 and CK7 Cocktail AC597-YCD CDX2-88+EPR1622Y+EPR1619Y/Mouse+Rabbit HX031-YCDE/HX032-YCDE CDX-2 and CK7 Cocktail AC596-YCD CDX2-88+EPR1619Y/Mouse+Rabbit HX031-YCDE/HX032-YCDE CEA AX365-YCD CEA88/Mouse None c-erbB-2 (HER-2/neu) AX471-YCDE EP1045Y HX031-YCDE c-erbB-2 (Her-2/neu) AX134-YCDE CB11/Mouse None c-erbB-2 (HER-2/neu) (New) AY726-YCDE EP3/Rabbit None c-erbB-3 (HER-3) AX319-YCD RTJ1/A2/Mouse HX031-YCDE/HX032-YCDE Chromogranin A AX126-YCD LK2H10/Mouse None Chromogranin A AX356-YCD PHE-5/Mouse HX031-YCDE Claudin 1 AW704-YCD Polyclonal/Rabbit HX031-YCDE Claudin-5 (New) AY718-YCD EP224/Rabbit HX032-YCDE c-myc Protein AX318-YCD 9E10/Mouse HX032-YCDE Collagen III AX167-YCD HWD1.1/Mouse None Collagen IV AX379-YCD COL-94/Mouse HX031-YCDE/HX032-YCDE Cyclin D1 AW447-YCD Polyclonal/Rabbit HX031-YCDE Cyclin D1 AX474-YCD EPR2241(IHC)-32/Rabbit HX032-YCDE Cyclin E2 AX519-YCD EP454Y/Rabbit HX032-YCDE Cytokeratin 4 AY705-YCD 6B10/Mouse None Cytokeratin 4 (New) AY717-YCD EP4/Rabbit HX031-YCDE Cytokeratin 5 AX484-YCD EPR1600Y/Rabbit HX031-YCDE Cytokeratin 5 & 6 AX588-YCD EPR1600Y & EPR1602Y/Rabbit HX031-YCDE Cytokeratin 5 & Cytokeratin 14 (New) AY730-YCDE EP24/EP61/Rabbit HX032-YCDE Cytokeratin 6 AX500-YCD EPR1602Y/Rabbit HX031-YCDE internationalcs@biogenex.com 13 Automated Systems Xmatrx® Reagents and Consumables Product Name Cat. No. Clone/Source Pre-treatment Cytokeratin 7 AX255-YCD OV-TL12/30/Mouse HX032-YCDE Cytokeratin 7 AX528-YCD EPR1619Y/Rabbit HX031-YCDE Cytokeratin 7 & 8 AX587-YCD OV-TL12/30 & C51/Mouse HX031-YCDE Cytokeratin 8 AX142-YCD C51/Mouse HX031-YCDE Cytokeratin 8 & 18 AX131-YCD 5D3/Mouse HX031-YCDE Cytokeratin 10 AX201-YCD DEK-10/Mouse HX031-YCDE Cytokeratin 13 AX132-YCD AE8/Mouse HX031-YCDE Cytokeratin 14 AX146-YCD LL002/Mouse HX031-YCDE Cytokeratin 15 AX485-YCD EPR1614Y/Rabbit HX031-YCDE Cytokeratin 16 AX486-YCD EP1615Y/Rabbit HX031-YCDE Cytokeratin 17 AX572-YCD E27/Mouse HX031-YCDE Cytokeratin 18 AX143-YCD DC-10/Mouse HX031-YCDE Cytokeratin 19 AX246-YCD RCK108/Mouse HX032-YCDE Cytokeratin 20 AX315-YCD IT-Ks20.8/Mouse HX031-YCDE Cytokeratin 20 AX557-YCD EPR1622Y/Rabbit HX031-YCDE Cytokeratin cocktail AX071-YCD AE1 and AE3/Mouse HX031-YCDE Cytokeratin cocktail, Broad spectrum AX273-YCD 34ßE12/C51/AE1/Mouse HX032-YCDE Cytokeratin cocktail, Broad spectrum AX372-YCD LL002+DEK-10+RCK108+OVTL12/30+C11/Mouse HX032-YCDE Cytokeratin High MW AX291-YCD 34ßE12/Mouse HX031-YCDE Cytokeratin High MW (Basic) AX133-YCD AE3/Mouse HX031-YCDE Cytokeratin Low MW AX075-YCD AE1/Mouse HX032-YCDE Cytokeratin Pan AX181-YCD Lu-5/Mouse HX031-YCDE Cytokeratin Pan AX357-YCD C11/Mouse HX031-YCDE Desmin AX072-YCD D33/Mouse None DOG 1 AX570-YCD 1.1/Mouse HX031-YCDE Dystrophin AX243-YCD Dy4/6D3/Mouse HX031-YCDE Dystrophin AX244-YCD Dy8/6C5/Mouse HX031-YCDE E-Cadherin AX390-YCD 36/Mouse HX031-YCDE E-Cadherin (New) AY725-YCD EP6/Rabbit HX032-YCDE EGFR AW335-YCDE Polyclonal/Rabbit HX031-YCDE/HX032-YCDE Ep-CAM AX489-YCD E144/Rabbit HX031-YCDE/HX032-YCDE Epithelial Membrane Antigen (EMA) AX057-YCD E29/Mouse HX031-YCDE/HX032-YCDE Epithelial Membrane Antigen (EMA) AX182-YCD Mc5/Mouse HX031-YCDE Epithelial-Specific Antigen AX316-YCD MOC-31/Mouse EK000-5K Estradiol AW038-YCD Polyclonal/Rabbit HX031-YCDE Estrogen Receptor AY710-YCDE EP-1/Rabbit None Estrogen Receptor AX368-YCDE ER88/Mouse HX031-YCDE Estrogen Receptor AX509-YCDE EPR703(2)/Rabbit HX032-YCDE Estrogen Receptor AX272-YCDE 1D5/Mouse HX032-YCDE Estrogen Receptor-beta AW385-YCD Polyclonal/Rabbit HX031-YCDE/HX032-YCDE Factor VIII-related antigen AX016-YCD F8 2.2.9/Mouse HX031-YCDE Factor XIII Subunit A AX516-YCD EP3372/Rabbit HX031-YCDE Factor XIII A AX337-YCD E980.1/Mouse HX031-YCDE Fascin AX488-YCD FCN01/Mouse HX031-YCDE Fibronectin AX569-YCD F14/Rabbit HX031-YCDE/HX032-YCDE Follicle Stimulating Hormone (FSH) AW026-YCD Polyclonal/Rabbit HX031-YCDE Gastrin AW019-YCD Polyclonal HX031-YCDE GCDFP 15 AX481-YCD EP1582Y/Rabbit HX031-YCDE Glial fibrillary acidic protein (GFAP) AX020-YCD GA-5/Mouse None Glial Fibrillary Acidic Protein (GFAP) AW020-YCD Polyclonal/Rabbit HX031-YCDE Glomerular epithelial Protein 1 (GLEPP-1) AX336-YCD 5C11/Mouse HX031-YCDE/HX032-YCDE Glucagon AW039-YCD Polyclonal/Rabbit HX031-YCDE 14 www.biogenex.com Automated Systems Xmatrx® Reagents and Consumables Product Name Cat. No. Clone/Source Pre-treatment Glut-1 AX505-YCD SPM498/Mouse HX031-YCDE/HX032-YCDE Glutathione S-transferase Pi (GST Pi) AW249-YCD Polyclonal/Rabbit HX031-YCDE/HX032-YCDE HSA AX550-YCD HSA/E8/Mouse HX031-YCDE Heat Shock Protein (HSP-70) AX289-YCD BRM-22/Mouse HX031-YCDE/HX032-YCDE Heat shock protein 27 (HSP 27) AX171-YCD G3.1/Mouse HX031-YCDE Hemoglobin A AW021-YCD Polyclonal/Rabbit HX031-YCDE HGH AW707-YCD Polyclonal/Rabbit HX031-YCDE HLA-DR AX154-YCDE LN3/Mouse HX031-YCDE/HX032-YCDE Human Chorionic Gonadotropin (HCG) Beta AX395-YCD M94138/Mouse HX031-YCDE IgA AW045-YCD Polyclonal/Rabbit HX031-YCDE/HX032-YCDE IgD AW440-YCD Polyclonal/Rabbit HX031-YCDE/HX032-YCDE IgG AW050-YCD Polyclonal/Rabbit HX031-YCDE IgG AX367-YCD IgG88/Mouse HX031-YCDE/HX032-YCDE IgM AW427-YCD Polyclonal/Rabbit HX031-YCDE/HX032-YCDE IgM AX366-YCD IgM88/Mouse HX031-YCDE/HX032-YCDE Inhibin Alpha AX446-YCD R1/Mouse HX031-YCDE Insulin AX029-YCD HB125/Mouse HX031-YCDE Insulin (New) AY735-YCD EP125/Rabbit None J chain AX374-YCD JC88/Mouse HX031-YCDE Kappa Light Chain AX369-YCD K88/Mouse HX031-YCDE Kappa light chain AX048-YCD L1C1/Mouse HX031-YCDE Ki-67 AX527-YCD EPR3611/Rabbit HX032-YCDE Ki-67 AX410-YCD K2/Mouse HX032-YCDE Ki-67 (New) AY727-YCD EP5/Rabbit None Ki-67 antigen AX370-YCD Ki88/Mouse HX032-YCDE Ki-67 antigen Proliferating cell AX297-YCD MIB-1/Mouse HX032-YCDE Ki-67 + Lambda Light Chain AC562-YCD K-2/Mouse+Polyclonal/Rabbit HX031-YCDE/HX032-YCDE Lambda Light chain AW049-YCD Polyclonal/Rabbit HX031-YCDE Lambda Light Chain (New) AY715-YCD EP172/Rabbit HX032-YCDE Laminin AW078-YCD Polyclonal/Rabbit HX031-YCDE/HX032-YCDE Lysozyme AW024-YCD Polyclonal/Rabbit HX031-YCDE Macrophage AX165-YCD LN5/Mouse HX031-YCDE Mast Cell Tryptase AX419-YCD AA1/Mouse HX031-YCDE Melan-A (MART-1) AX361-YCD A103/Mouse HX031-YCDE Melanoma AX001-YCD HMB45/Mouse HX031-YCDE Melanoma-Associated Antigen AX077-YCD NKI/C3 HX031-YCDE/HX032-YCDE Melanoma gp100 AX536-YCD gp100/D5/Mouse HX031-YCDE Mesothelin AX433-YCD 5B2/Mouse HX031-YCDE MiTF AX554-YCD MiTF/A13/Mouse HX032-YCDE MLH1 AY703-YCD ES05/Mouse HX031-YCDE MMP9 AX504-YCD EP1255Y/Rabbit HX031-YCDE MSH2 (New) AY743-YCD SP46/Rabbit None MSH6 AX454-YCD 2D4B5/Mouse HX032-YCDE MUC4 AX455-YCD 1G8/Mouse HX031-YCDE/HX032-YCDE MUC5AC AX456-YCD 45M1/Mouse HX031-YCDE Mucin 1 AX564-YCD EPR1023/Rabbit HX031-YCDE Mucin-2 (MUC-2) AX358-YCD CCP58/Mouse HX031-YCDE Multi-Drug Resistance Marker (P-Glycoprotein) AX391-YCD MDR88/Mouse HX031-YCDE/HX032-YCDE Muscle actins AX381-YCD Actin 88 Cocktail/Mouse HX031-YCDE internationalcs@biogenex.com 15 Automated Systems Xmatrx® Reagents and Consumables Product Name Cat. No. Clone/Source Pre-treatment Myelin Basic Protein AX380-YCD MBP88/Mouse None Myeloid Specific Antigen AX216-YCD BM-3/Mouse HX031-YCDE Myeloid Specific antigen (BM-1) AX164-YCD BM-1/Mouse HX032-YCDE Myeloperoxidase (MPO) AW496-YCD Polyclonal/Rabbit HX031-YCDE Myf4 AX432-YCD LO26/Mouse HX032-YCDE Myoglobin AX012-YCD MG-1 HX031-YCDE Myoglobin AW012-YCD Polyclonal/Rabbit HX031-YCDE Myosin, Skeletal Muscle AX109-YCD MY-32/Mouse HX031-YCDE/HX032-YCDE Myosin Heavy Chains, Smooth Muscle AX331-YCD SMMS.1/Mouse HX032-YCDE N-Cadherin AX565-YCD EPR1792Y/Rabbit HX031-YCDE/HX032-YCDE Napsin A AY701-YCD IP64/Mouse HX031-YCDE Neurofilament AX073-YCD NE-14/Mouse None Neuron specific enolase (NSE) AX055-YCD MIG-N3/Mouse None NGFR AX522-YCD EP1039Y/Rabbit HX031-YCDE NGFR (New) AY738-YCD EP31/Rabbit HK032-YCDE Oct 2 AX558-YCD EPR542/Rabbit HX032-YCDE Oct 4 (New) AY724-YCD EP143/Rabbit HK032-YCDE Osteonectin AX387-YCD OST1/Mouse HX031-YCDE p105 Proliferation-Associated Nuclear Antigen AX317-YCD 2B3/Mouse HX032-YCDE p21/WAF1 AX434-YCD 4D10/Mouse HX032-YCDE p27 (Kip1) AX492-YCD Y236/Rabbit HX032-YCDE p27 (Kip1) AX396-YCD DCS72/Mouse HX032-YCDE p34cdc2 (Cyclin Dependent Kinase) AX301-YCD POH-1/Mouse HX031-YCDE/HX032-YCDE P504S AX449-YCDE 13H4 HX031-YCDE P504S AX538-YCD RBT-AMACR/Rabbit HX031-YCDE p53 (New) AY728-YCD EP9/Rabbit None p53 Protein AX195-YCD BP53-12-1/Mouse HX032-YCDE p53 Protein AX239-YCD DO7/Mouse HX032-YCDE p53 Protein AX240-YCD 1801/Mouse HX032-YCDE p63 Protein AX418-YCD 4A4/Mouse HX032-YCDE p63+CK5 AC602-YCD 4A4/Mouse + EPR1600Y/Rabbit HX031-YCDE/HX032-YCDE PAP AX532-YCD A40010/Mouse HX031-YCDE Papillomavirus type 16 (HPV-16) AX362-YCDE Cam Vir-1/Mouse HX032-YCDE PAX5 AX457-YCD ZP007/Mouse HX032-YCDE Paxillin AX521-YCD Y113/Rabbit HX031-YCDE/HX032-YCDE PDCD4 AX524-YCD EPR3432/Rabbit HX031-YCDE/HX032-YCDE PDGF AW376-YCD PDGF88 None PDGFR Beta AX463-YCD Y92/Rabbit HX031-YCDE PGP9.5 (New) AY736-YCDE 3D9/Mouse HK031-YCDE PIN4 (p63 + CK HMW + P504S) AX448-YCDE Mouse4A4+Mouse34βE12+Rabbit13H4 HX032-YCDE Placental alkaline phosphatase (PLAP) AX228-YCD PL8-F6/Mouse None Placental Lactogen (hPL) AW040-YCD Polyclonal/Rabbit HX031-YCDE Platelet derived growth factor (PDGF) AX376-YCD PDGF88/Mouse HX031-YCDE PMS2 AX577-YCD EPR3947/Rabbit HX032-YCDE Prealbumin/Transthyretin AX501-YCD EP2929Y/Rabbit HX031-YCDE Progesterone receptor AX172-YCDE 1Ab/Mouse HX032-YCDE Progesterone receptor AX328-YCD PR88/Mouse HX032-YCDE Progesterone receptor AY711-YCDE EP2/Rabbit HX031-YCDE Prolactin AX031-YCD ME.121/Mouse None Proliferating cell nuclear antigen (PCNA) AX252-YCD PC10/Mouse HX032-YCDE Prostate specific acid phosphatase (PSAP) AX013-YCDE B01-94-21M-NA/Mouse None Prostate specific antigen (PSA) AX014-YCDE ErPr-8/Mouse None 16 www.biogenex.com Automated Systems Xmatrx® Reagents and Consumables Product Name Cat. No. Clone/Source Pre-treatment pS2 Estrogen inducible protein AX190-YCD PS2.1/Mouse HX031-YCDE PSMA (New) AY714-YCD EP192/Rabbit HK032-YCDE RCC AX543-YCD RCC-26/Mouse None S100 beta (New) AY713-YCD EP32/Rabbit HK032-YCDE S-100 Protein AX058-YCD 15E2E2/Mouse None S-100 Protein AW058-YCD Polyclonal/Rabbit HX031-YCDE/HX032-YCDE S100P (New) AY712-YCD EP186/Rabbit None Sarcomeric Actin AX511-YCD ZMSA-5/Mouse HX031-YCDE Secretin AW067-YCD Polyclonal/Rabbit HX031-YCDE Stat 5 Alpha AX480-YCD E289/Rabbit HX031-YCDE/HX032-YCDE Substance P AW069-YCD Polyclonal/Rabbit HX031-YCDE/HX032-YCDE Survivin AX503-YCD EP2880Y/Rabbit HX032-YCDE Synaptophysin AX363-YCD Snp88/Mouse HX031-YCDE TAU AX412-YCD Tau-2/Mouse HX031-YCDE Tau AX459-YCD TAU-5/Mouse HX031-YCDE Terminal deoxynucleotidyl transferase (TdT) AX373-YCD TdT88/Mouse HX031-YCDE Terminal deoxynucleotidyl Transferase (TdT) AW373-YCD Polyclonal/Rabbit HX032-YCDE TFPU.1 AX520-YCD EPR3159Y/Rabbit HX032-YCDE Thyroglobulin AX032-YCD 2H11/Mouse None Thyroid stimulating hormone (TSH) AX033-YCD 5404/Mouse None Thyroid stimulating hormone (TSH) AW033-YCD Polyclonal/Rabbit HX031-YCDE Thyroxine AX034-YCD D5/Mouse HX031-YCDE/HX032-YCDE TIA-1 AX529-YCD 2G9A10F5/Mouse HX031-YCDE Topo 2 Alpha AX576-YCD EP1102Y/Rabbit HX032-YCDE Transferrin AX025-YCD HT1/13.6.3/Mouse HX031-YCDE Transforming growth factor (TGF), alpha AX377-YCD TGF-α88/Mouse HX031-YCDE TTF-1 AX397-YCD 8G7G3/1/Mouse HX031-YCDE TTF-1 + CK20 + CK7 AC605-YCD 8G7G3/1+EPR1622 Y+EPR1619Y/Mouse+Rabbit HX031-YCDE/HX032-YCDE TTF-1 + GCDFP-15 AC604-YCD 8G7G3/1+EP1582Y/Mouse+Rabbit HX031-YCDE/HX032-YCDE TTF1+CK5/6 AC563-YCD 8G7G3/1/Mouse+EPR1600Y&EPR1602Y/Rabbit HX031-YCDE/HX032-YCDE Tumor-associated glycoprotein (TAG-72) AX054-YCD B72.3/Mouse None Tumor-Associated glycoprotein (TAG-90, BCA) Dx AX005-YCD B6.2/Mouse HX031-YCDE Tyrosinase AX535-YCD Ty/G5/Mouse HX031-YCDE VEGF AW483-YCD Polyclonal/Rabbit HX031-YCDE Villin AX525-YCD ID2C3/Mouse HX031-YCDE Vimentin AX074-YCD V9/Mouse None Vimentin (Non-hematopoietic) AX163-YCD LN6/Mouse None VIP AW530-YCD Polyclonal/Rabbit HX031-YCDE Wilm’s Tumor AX584-YCD CAN-R9(IHC)-56-2/Rabbit HX032-YCDE ZAP-70 AX544-YCD ZAP70-C3/Mouse HX031-YCDE ZAP-70 AX466-YCD YE291/Rabbit HX031-YCDE/HX032-YCDE For pretreatment information, please contact us. internationalcs@biogenex.com 17 Automated Systems Xmatrx® Reagents and Consumables Immunohistochemistry - Detection Kits The XVizTM Detection System All reagents are packed in RFID tagged vials especially designed for use on Xmatrx® Automated Staining Systems to ensure accurate identification, proper reagent inventory management and staining up to 200 slides. Product Name XViz TM ® Detection Kit for Xmatrx Diagnostics Pack size Cat. No. 200 slides QD550-YCDE 200 slides QD550-YCX EZ-ARTM Elegance solutions (1 X 16 ml each of solutions 1, 2, and 3) 3 X 16 ml Peroxide Block, 3 X 16 ml Power BlockTM, 1 X 16 ml Super Enhancer, 1 X 16 ml Polymer HRP, 4 X 11 ml DAB Buffer, 1 X 4 ml DAB chromogen, 3 X 16 ml Hematoxylin XVizTM Detection Kit for Xmatrx® Infinity TM EZ-AR Elegance Solution (1x16 ml solution), 4x16 ml Peroxide Block, 1x15 ml Super enhancer, 5x11 ml DAB buffer, 1x4 ml DAB Chromogen, 1x15 ml Polymer HRP, 4x16 ml Hematoxylin, 1x21 ml Power Block. Super SensitiveTM One-step Polymer-HRP Detection Kit This kit is designed with the proprietary technology which provides superior sensitivity, specificity and very short protocol. The innovative secondary antibody-polymer conjugate consists of multiple small HRP active sites, which enables clean and intense, nuclear, cytoplasmic, and membrane stains. Product Name Pack size TM Super Sensitive One-step Polymer-HRP Detection Kit/DAB 200 Slides Contents Cat. No. TM EZ-AR Elegance solutions (1 x 16ml each of solutions 1, 2, and 3), 3 x 16ml Peroxide Block, 3 x 16ml Power BlockTM, 1 x 16ml Polymer HRP, 4 x 11ml DAB Buffer, 1 x 4ml DAB chromogen, 3 x 16ml Hematoxylin QD610-YADE XVizTM Double Staining Polymer Detection Kits Product Name XViz™ Double Staining Polymer Detection Kit I/DAB&Fast Red XViz™ Double Staining Polymer Detection Kit II/DAB&Fast Red Pack Size Contents Cat. No. 100 Slides 2 X 10 ml Power Block, 2 X 10 ml Peroxide Block, 4 X 5 ml DAB Buffer 1 X 3 ml Liquid DAB Chromogen, 1 X 7 ml EZ-AR™ Elegance Solutions (1, 2 And 3), 1 X 7 ml Mouse Negative Control, 1 X 7 ml Rabbit Negative Control, 1 X 7 ml Anti Rabbit Poly-Hrp + Anti Mouse Poly-Hrp, 2 X 10 ml Hematoxylin, 2 X 14 ml Permanent Fast Red A, 2 X 14 ml Permanent Fast Red B QS200-YADE 100 Slides 2 X 10 ml Power Block, 2 X 10 ml Peroxide Block, 4 X 5 ml DAB Buffer 1 X 3 ml Liquid DAB Chromogen, 1 X 7 ml EZ-AR™ Elegance Solutions (1, 2 And 3), 1 X 7 ml Mouse Negative Control, 1 X 7 ml Rabbit Negative Control, 1 X 7 ml Anti Mouse Poly-Hrp + Anti Rabbit Poly-Hrp, 2 X 10 ml Hematoxylin, 2 X 14 ml Permanent Fast Red A, 2 X 14 ml Permanent Fast Red B QS400-YADE Auto DAB kit for Xmatrx Elite Product Name Pack size Contents Cat. No. Auto DAB kit for Xmatrx Elite 200 slides 1 vial of DAB chromogen XT150-YCDE 5 empty vials for DAB mixing 18 www.biogenex.com Automated Systems Xmatrx® Reagents and Consumables Antigen Retrieval Solutions The EZ-ARTM Elegance Solutions possess unique properties that enable optimal dewaxing, rehydration, and antigen retrieval in formalinfixed, paraffin-embedded tissue sections. These solutions facilitate the production of highly reproducible and superior quality stains in a considerably short period of time without compromising the morphology and antigenicity of the tissue. Product Name Pack size Product Description Cat. No. EZ-AR™ 1 Elegance 200 slides EZ-AR™ 1 Elegance is a Citra based solution. Works at 100°C HX031-YCDE EZ-AR™ 2 Elegance 200 slides EZ-AR™ 2 Elegance is an EDTA based solution. Works at 100°C HX032-YCDE EZ-AR™ 3 Elegance 200 slides EZ-AR™ 3 Elegance is a Citra based solution. Works at 100°C HX033-YCDE EZ-AR™ 4 Elegance 200 slides EZ-AR™ 4 Elegance is an Tris based solution. Works at 100°C HX034-YCDE Product Name Pack size Product Description Cat. No. EZ-AR™ 1 Elegance 200 slides EZ-AR™ 1 Elegance is a Citra based solution. Works at 100°C HX031-YCX EZ-AR™ 2 Elegance 200 slides EZ-AR™ 2 Elegance is an EDTA based solution. Works at 100°C HX032-YCX EZ-AR™ 3 Elegance 200 slides EZ-AR™ 3 Elegance is a Citra based solution. Works at 100°C HX033-YCX EZ-AR™ 4 Elegance 200 slides EZ-AR™ 4 Elegance is an Tris based solution. Works at 100°C HX034-YCX Enzymatic Pre-treatment Solutions Product Name Pack size Cat. No. Pepsin 4-Pack: 4 vials of Lyophilized Enzyme Powder, 4 x 6 mL Reconstitution Buffer 200 slides EK000-10X Trypsin 4-Pack: 4 vials of Lyophilized Enzyme Powder, 4 x 6 mL Reconstitution Buffer 200 slides EK001-10X Protease XXIV 4-Pack: 4 vials of Lyophilized Enzyme Powder, 4 x 6 mL Reconstitution Buffer 200 slides EK002-10X in situ Hybridization Kits and Probes The XISH Detection Kit is designed for using with fluorescein labeled probes. It enables accurate detection of specific DNA and mRNA sequences in routine paraffin sections/cell smears. ISH Probes Probes are packaged with RFID vials for staining up to 25 slides. Product Name Pack size Intended Use Cat. No. Alu II DNA 25 slides Positive control probe for detection of primate DNA sequence repeat PR026-YADE CerviPro HPV 14 25 slides Detection of high risk genotypes of human papillomavirus PR251-YADE CerviPro HPV Type 16/18 25 slides Detection of HPV types 16 and 18 PR250-YADE Cyclin D1 25 slides Detection of CyclinD1 mRNA PR216-YADE Epstein Barr Virus Early RNA (EBER) 25 slides Detection of latent EBV infection PR205-YADE Kappa 25 slides Detection of Kappa light chain mRNA PR214-YADE Lambda 25 slides Detection of Lambda light chain mRNA PR215-YADE Oligo dT 25 slides Assessment of mRNA preservation PR217-YADE Retinoblastoma 25 slides Detection of Retinoblastoma mRNA PR225-YADE Beta-Actin 25 slides Internal standard for ISH and Northern blot PR1055-YADE Note: See Page No. 151 for New ISH Probes (To be launched) One Step ISH Detection Kit Product Name TM XISH One Step Polymer-HRP ISH Detection System Pack size Probe Type Cat. No. 50 slides Fluorescein Labeled DF400-YADE 1 x10ml Power Block, 1 x 10 ml Peroxide Block, 4 x 5 ml DAB Buffer, 1 x 3ml Liquid DAB Chromogen, 1 x 3ml One step Poly-HRP Reagent; 1 x 10ml Hematoxylin; 1 x 3ml Proteinase K; 1 x 3ml Nucliec Acid Retrieval Solution; 2 x 10ml Wash Solution A; 2 x 10ml Wash Solution B; 2 x 10ml Wash Soution E; 2 x 10ml Wash Solution F; 1 x 3ml Anti-Flourescein Antibody internationalcs@biogenex.com 19 Automated Systems Xmatrx® Reagents and Consumables Empty Reagent Vials Product Name Pack size Cat. No. User defined Empty RFID tagged vials- Two step IHC Each XT077-AX0601 to XT077-AX0800 User defined Empty RFID tagged vials- One step IHC Each XT077-AX0801 to XT077-AX0999 User defined Empty RFID tagged vials- ISH Probes Each XT079-PR0050 to XT079-PR0099 Consumable Kit Product Name Pack size Cat. No. Xflex Consumable Kit 200 slides XT132-YCD 100 slides XT144-YAD 200 slides XT148-YCD 100 slides XT147-YAD 3 x 72 nos 25 X 40 mm Double Barrier Slides, 2 x 500 nos 25 x 40 mm Coverslips, 9 x 96 nos Small Pipette tips, 2 x 192 Large Pipette tips (1 ml) ISH Consumable Kit-Xmatrx® 2 x 72 nos 25 x 25 mm Double Barrier Slides, 1 x 900 Nos of 25 x 25 mm Coverslips, 2 x 192 Large Pipette Tips (1 ml), 1 x 960 Nos of Pipette Tips (200µL) Xflex Ultra Consumable Kit 3 x 72 Nos of 25 X 40 mm Double Barrier Slides, 2 x 500 Nos of 25 X 40 CoverSlips, 1 X 960 Nos of Pipette Tips (200µL), 2 x 192 Large Pipette Tips (1 ml) FISH Consumable Kit ® 2 x 72 nos 18 x 18 mm Barrier slides, 2 x 175 nos of 18 x 18 mm coverslips Xmatrx , 1 x 960 pipette tips (200µL), 2 x 192 Large pipette tips (1ml) Xmatrx® ELITE and Infinity Consumables Product Name Pack Size Cat. No. Barrier Slides, 18x18 mm, 2-zone, Xmatrx® ELITE & Infinity 1440 Slides/Case XT114-CL Barrier Slides, 18x18 mm, 2-zone, Xmatrx® ELITE & Infinity 72 Slides/Box XT114-SL ® 1440 Slides/Case XT128-CL ® 72 Slides/Box XT128-SL ® 1440 Slides/Case XT108-CL ® Barrier Slides, 25X25 mm, Xmatrx ELITE & Infinity 72 Slides/Box XT108-SL Barrier Slides, 25X40 mm, Xmatrx® ELITE ISH & Infinity 1440 Slides/Case XT134-CL Barrier Slides, 25X40 mm, Xmatrx® ELITE ISH & Infinity 72 Slides/Box XT134-SL Coverslips, 18x18 mm, Xmatrx® ELITE & Infinity 1750 Coverslips/Case XT121-XBK Coverslips, 18x18 mm, Xmatrx® ELITE & Infinity Barrier Slides, 18x18 mm, Xmatrx ELITE & Infinity Barrier Slides, 18x18 mm, Xmatrx ELITE & Infinity Barrier Slides, 25X25 mm, Xmatrx ELITE & Infinity 175 Coverslips/Box XT121-YBX ® 90 Coverslips/Box XT122-90X ® 900 Coverslips/Case XT122-YQK ® 50 Coverslips/Box XT118-50X 500 Coverslips/Box XT118-YRK 24/Pack XT126-24V Coverslips, 25x25 mm, Xmatrx Infinity & ELITE ISH Coverslips, 25x25 mm, Xmatrx Infinity & ELITE ISH Coverslips, 25x40 mm, Xmatrx ELITE & Infinity ® Coverslips, 25x40 mm, Xmatrx ELITE & Infinity ® Reagent Vials, Brown, 20ml, Xmatrx Reagent Vials, Translucent, 20ml 24/Pack XT026-V24 Pipette Tips, 1 ml, Xmatrx® ELITE & Infinity 960 Tips/Case XT104-05X Pipette Tips, 1 ml, Xmatrx® ELITE & Infinity 192 Tips/Box XT105-01X 20 www.biogenex.com Automated Systems Xmatrx® Reagents and Consumables Product Name Pack Size Cat. No. 960 Tips/Box XT146-01X Pipette Tips, 200ul, Xmatrx Infinity & ELITE 4800 Tips/Case XT145-05X Reagent Vial Insert, 2 ml 24/Pack XT149-V24 Empty Reagent Vials for Xmatrx - White 24/Pack XT077-V24 Empty Reagent Vials for Xmatrx - Brown 24/Pack XT078-V24 ® Pipette Tips, 200 ul, Xmatrx Infinity & ELITE ® Ancillary Reagents DeWax Solutions† BioGenex X-DeWaxTM Solution is a “one-step” product that simultaneously enables the removal of paraffin and allows rehydration of the tissue with a single reagent. In the past, formalin-fixed, paraffin-embedded tissue sections were traditionally deparaffinized with highly toxic, noxious chemicals (i.e. xylene, equivalents). BioGenex, a pioneer in the Immunohistochemistry technology, offers a xylene-free product that removes the paraffin from mounted tissue slides easily and rapidly. Product Name Pack size Cat. No. X-DeWax™ Solution (Concentrated) 1000 ml HX016-XAK† X-DeWax™ Solution (Concentrated) 1 Gallon HX016-XEK† XMount™ Product Name Pack size Cat. No. XMount™ for Xmatrx® 40 slides HX035-04X XMount™ for Xmatrx® 100 slides HX035-40D ® 100 slides HX035-10X XMount™ for Xmatrx Wash Buffers XWashTM Buffer provides optimal staining with minimal background. Product Name Pack size Cat. No. TM 500 ml HX020-YIK TM XWash , ISH Wash Buffer, 20X concentrated 500 ml HX017-YIK Super Sensitive™ SS Wash Buffer, 20X concentrated 500 ml HK583-5KE XWash , IHC Wash Buffer, 20X concentrated Xmatrx® FISH Application Product Name ® Xmatrx FISH Software † Cat. No. 4812-00089 U.S. Patent No. 6,632,598; U.S. Patent No. 7, 070, 951; Japanese Patent No. 3532571; European Patent No. 0698118B1. internationalcs@biogenex.com 21 Automated Systems Xmatrx® Reagents and Consumables Consumables for FISH Product Name Pack Size Cat. No. Barrier Slides, 18x18 mm, 2-zone, Xmatrx 1440 Slides/Case XT114-CL Barrier Slides, 18x18 mm, 2-zone, Xmatrx® 72 Slides/Box XT114-SL Barrier Slides, 18x18 mm, Xmatrx 1440 Slides/Case XT128-CL Barrier Slides, 18x18 mm, Xmatrx® 72 Slides/Box XT128-SL ® 72 Slides/Box XT108-SL ® 1440 Slides/Case XT108-CL ® 1440 Slides/Case XT134-CL ® 72 Slides/Box XT134-SL 1750 Coverslips/Case XT121-XBK ® ® Barrier Slides, 25x25 mm, Xmatrx Barrier Slides, 25x25 mm, Xmatrx Barrier Slides, 25x40 mm, Xmatrx Barrier Slides, 25x40 mm, Xmatrx ® Coverslips, 18x18 mm, Xmatrx ® Coverslips, 18x18 mm, Xmatrx 175 Coverslips/Box XT121-YBX Coverslips, 25x25 mm, Xmatrx® 900 Coverslips/Case XT122-YQK Coverslips, 25x25 mm, Xmatrx® 90 Coverslips/Box XT122-90X Coverslips, 25x40 mm, Xmatrx 500 Coverslips/Box XT118-YRK Coverslips, 25x40 mm, Xmatrx® 50 Coverslips/Box XT118-50X Reagent Vials, Translucent, 20 ml 24/Pack XT026-V24 Pipette Tips, 1 ml 960 Tips/Case XT104-05X Pipette Tips, 1 ml 192 Tips/Box XT105-01X Pipette Tips, 200 ul 960Tips/Box XT146-01X Pipette Tips, 200 ul 4800 Tips/Box XT145-05X Reagent Vial Insert, 2 ml 24/Pack XT149-V24 Reagent Vial, Brown, 20ml 24/Pack XT126-24V FISH Consumable Kit-Xmatrx® 100 slides XT147-YAD ® Note: Unless specified otherwise, all products listed in this section are for Laboratory Use Only. † U.S. Patent No. 6,632,598; U.S. Patent No. 7, 070, 951; Japanese Patent No. 3532571; European Patent No. 0698118B1. 22 www.biogenex.com Automated Systems i6000™ Automated Staining System Product Name Cat. No. i6000 TM Automated Staining System AS6030 (Diagnostics), AS6040 (Infinity) i6000 TM Software 4812-00093 (Diagnostics), 4812-00094 (Infinity) Reagents and Consumables Immunohistochemistry - Reagents Primary antibodies in 10 ml Barcoded vials Primary Antibodies Product Name Cat. No. Clone/Source Pre-treatment ACTH AM487-10M AH26/Mouse HK522-XAK Actin, Muscle-Specific AM090-10M HHF35/Mouse None Actin, Smooth Muscle AM128-10M 1A4/Mouse None Alpha-Fetoprotein (AFP) AM008-10M C3/Mouse None Androgen receptor AM256-10ME F39.4.1/Mouse HK087-5KE Apolipoprotein E AN567-10M EP1373Y/Rabbit HK081-5KE B cell AM158-10M MB2/Mouse None B Lymphocyte antigen 36 (BLA.36) AM231-10M A27-42/Mouse None BcI-2 Oncoprotein AM287-10M bcl-2/100/Mouse HK087-5KE Bcl 2 AN541-10M E17/Rabbit HK521-XAK Bcl 2 AM723-10M EP36/Rabbit HK058-5K Bcl-6 AM708-10M LN22/Mouse HK058-5K Bcl-x AN462-10M E18/Rabbit HK521-XAK Beta-Catenin AN510-10M E247/Rabbit HK521-XAK BOB-1 (New) AN731-10ME SP92/Rabbit HK522-XAK Breast Cancer Antigen BCA 225 AM135-10M CU18/Mouse None CA19-9 AM424-10M C241:5:1:4/Mouse HK081-5KE CA 125 (Ovarian Tumor Marker) AM429-10M Ov185:1/Mouse HK058-5K Caldesmon AN507-10M E89/Rabbit HK521-XAK Calponin-1 AN502-10M EP798Y/Rabbit HK081-5KE Calretinin AR413-10R Polyclonal/Rabbit HK081-5KE Calretinin AM583-10M 2 E7/Mouse HK081-5KE, HK080-5K Carcinoembryonic antigen (CEA) AM009-10M B01-94-11M-P/Mouse None Carcinoembryonic antigen (CEA) AM365-10M CEA-88/Mouse None Catenin (p120) AN523-10M YE372/Mouse HK521-XAK Catenin Delta 1 (p120) AR706-10M Polyclonal/Rabbit HK080-5K CD1a AM490-10M O10/Mouse HK521-XAK CD3 Epsilon AN477-10M EP449E/Rabbit HK521-XAK CD3 (T cell) AM322-10M PS1/Mouse HK058-5K CD3 + CD20 AC559-10M EP449E+L26/Mouse & Rabbit HK521-XAK CD4 AM421-10M 4B12/Mouse HK058-5K CD4 (New) AN722-10M EP204/Rabbit HK057-5KE CD4 + CD8 AC595-10M 4B12+EP1150Y/Mouse+Rabbit HK058-5K CD5 AM430-10M 4C7/Mouse HK058-5K CD5 AN512-10M EP2952/Rabbit HK522-XAK CD7 AM702-10M LP15/Mouse HK080-5K CD8 AM422-10M 1A5/Mouse HK058-5KE CD8 AN513-10M EP1150Y/Rabbit HK081-5KE CD8 (New) AN740-10M SP16/Rabbit HK081-5K CD10 AM451-10M 56C6/Mouse HK521-XAK internationalcs@biogenex.com 23 Automated Systems i6000™ Reagents and Consumables Product Name Cat. No. Clone/Source Pre-treatment CD10 + Cyclin D1 AC561-10M 56C6 + EPR2241/Mouse & Rabbit HK521-XAK CD11b/ITAM AN546-10M EPR1344/Rabbit HK521-XAK CD11c AN555-10M EP1347Y/Rabbit HK081-5KE CD14 AN515-10M EPR3653/Rabbit HK521-XAK CD15 (Blood group antigen Lewis X) AM302-10M BRA4F1/Mouse None CD19 (New) AN729-10M EP169/Rabbit HK081-5KE CD20 AM537-10M CD20/C23/Mouse HK081-5KE CD20 (B cell) AM238-10M L-26/Mouse None CD21 AN497-10M EP3093/Rabbit HK522-XAK CD23 AR460-10R Polyclonal/Rabbit HK521-XAK CD25 AM453-10M 4C9/Mouse HK521-XAK CD30 (Ki-1 Antigen) AM327-10M Ber-H2/Mouse HK058-5K CD30 (Ki-1 Antigen) AM351-10M HRS-4/Mouse HK081-5KE CD31 AM232-10M JC/70A/Mouse EK000-10K CD34 (Endothelial cell) AM236-10M QBend/10/Mouse None CD35 AM431-10M RLB25/Mouse HK058-5K CD35 (New) AN741-10M SP191/Rabbit HK522-XAK CD41/Integrin Alpha IIb (New) AN732-10ME EP178/Rabbit HK057-5KE CD45 (Leukocyte Common Antigen, LCA) AM111-10M PD7/26/16&2B11/Mouse None CD45 cocktail (Leukocyte Common Antigen, LCA) AM371-10M MEM55-LJ 27.9/Mouse None CD45RA (B cell) AM157-10M MB1/Mouse None CD45RO (T cell) AM113-10M UCHL-1/Mouse None CD48 (New) AN721-10ME EP148/Rabbit HK522-XAK CD53 (New) AN734-10M EP179/Rabbit HK081-5KE CD61 AN482-10M EP2417Y/Rabbit HK522-XAK CD63 (New) AN720-10ME EP211/Rabbit HK522-XAK CD68 AM549-10M CD68/G2/Mouse HK522-XAK CD68 AM416-10M KP1/Mouse HK081-5KE CD79a AM414-10M 11E3/Mouse HK081-5KE CD79a (New) AN719-10M EP82/Rabbit HK081-5KE CD90 (New) AN733-10M EP56/Rabbit HK058-5KE CD95 (New) AN742-10M EP208/Rabbit HK522-XAK CD99 AN518-10M EPR3097Y/Rabbit HK521-XAK CD99 AM355-10M HO36.1.1/Mouse HK058-5K CD103 (New) AN739-10ME EP206/Rabbit HK522-XAK CD117 AM423-10M T595/Mouse HK081-5KE CD117 AM465-10M YR145/Rabbit HK081-5KE CD146 (New) AN716-10M EP54/Rabbit HK081-5KE CD205 (New) AN737-10ME EP176/Rabbit HK081-5KE CD227 (MUCIN 1) AM534-10M VU-4H5/Mouse HK521-XAK CDX2 AM392-10M CDX2-88/Mouse HK081-5KE CDX2 + CK7 AC596-10M CDX-2-88+EPR1619Y/Mouse+Rabbit HK081-5KE CDX2 + CK20 + CK7 AC597-10M CDX-2-88+EPR1622Y+EPR1619Y/M+R HK081-5KE c-erbB-2 (HER-2/neu) AN471-10ME EP1045Y/Rabbit None c-erbB-2 (HER-2/neu) AM134-10ME CB11/Mouse None c-erbB-2 (HER-2/neu) (New) AN726-10ME EP3/Rabbit HK081-5KE Chromogranin A AM126-10M LK2H10/Mouse None Chromogranin A AM356-10M PHE-5/Mouse HK058-5K Claudin 1 AR704-10M Polyclonal/Rabbit HK080-5K Claudin-5 (New) AN718-10M EP224/Rabbit HK081-5KE Collagen IV AM379-10M COL-94/Mouse EK000-5K Cyclin D1 AN474-10M EPR2241(IHC)-32/Rabbit HK521-XAK 24 www.biogenex.com Automated Systems i6000™ Reagents and Consumables Product Name Cat. No. Clone/Source Pre-treatment Cyclin D1 AR447-10R Polyclonal/Rabbit HK081-5KE Cyclin E2 AN519-10M EP454Y/Rabbit HK521-XAK Cytokeratin 4 AM705-10M 6B10/Mouse HK087-5KE HK081-5KE Cytokeratin 4 (New) AN717-10M EP4/Rabbit Cytokeratin 5 AN484-10M EPR1600Y/Rabbit HK081-5KE Cytokeratin 5 & 6 AN588-10M EPR1600Y & EPR1602Y/Rabbit HK081-5KE Cytokeratin 5 & Cytokeratin 14 (New) AN730-10ME EP24/EP61/Rabbit HK081-5KE Cytokeratin 6 AN500-10M EPR1602Y/Rabbit HK081-5KE Cytokeratin 7 AM255-10M OV-TL12/30/Mouse EK000-10K Cytokeratin 7 AN528-10M EPR1619Y/Rabbit HK522-XAK Cytokeratin 7 & 8 AM587-10M OV-TL12/30 & C51/Mouse HK081-5KE Cytokeratin 8 AM142-10M C51/Mouse HK167-5K Cytokeratin 8 & 18 AM131-10M 5D3/Mouse EK000-10K Cytokeratin 14 AM146-10M LL002/Mouse HK167-5K Cytokeratin 15 AN485-10M EPR1614Y/Rabbit HK521-XAK Cytokeratin 16 AN486-10M EP1615Y/Rabbit HK521-XAK Cytokeratin 17 AM572-10M E27/Mouse HK522-XAK Cytokeratin 19 AM246-10M RCK108/Mouse EK000-10K Cytokeratin 20 AM315-10M IT-Ks20.8/Mouse EK002-10K Cytokeratin 20 AN557-10M EPR1622Y/Rabbit HK081-5KE Cytokeratin Cocktail AM071-10M AE1 and AE3/Mouse EK001-10K Cytokeratin Cocktail, Broad spectrum AM372-10M LL002+DEK-10+RCK108+OVTL12/30+C11/Mouse EK000-10K Cytokeratin Low MW AM075-10M AE1/Mouse EK000-10K Cytokeratin, High MW AM291-10M 34ßE12/Mouse EK000-10K Desmin AM072-10M D33/Mouse None DOG 1 AM570-10M 1.1/Mouse HK081-5KE E-Cadherin AM390-10M 36/Mouse HK058-5K E-Cadherin (New) AN725-10M EP6/Rabbit HK081-5KE EGFR (Pan) AN473-10ME EP38Y/Rabbit None Ep-CAM AN489-10M E144/Rabbit HK087-5KE Epithelial Membrane Antigen (EMA) AM057-10M E29/Mouse None Epithelial Membrane Antigen (EMA) AM182-10M Mc5/Mouse None Epithelial-Specific Antigen AM316-10M MOC-31/Mouse HK167-5K Estrogen Receptor (ER Alpha) AN509-10ME EPR703(2)/Rabbit None Estrogen Receptor AM272-10ME 1D5/Mouse HK081-5KE Estrogen Receptor AR385-10R Polyclonal/Rabbit HK080-5K Estrogen Receptor AN710-10ME EP1/Rabbit HK081-5KE Estrogen Receptor (InSite® ER) AM368-10ME ER88/Mouse HK080-5K Factor VIII-related antigen AM016-10M F8 2.2.9/Mouse EK000-10K HK521-XAK Factor XIIIa AN516-10M EP3372/Rabbit Factor XIII Subunit A AM337-10M E980.1/Mouse HK081-5KE Fascin AM488-10M FCN01/Mouse HK087-5KE Fibronectin AN569-10M F14/Rabbit HK081-5KE Follicle Stimulating Hormone (FSH) AM026-10M 83/12/2A8 2C7/Mouse None GCDFP-15 AN481-10M EP1582Y/Rabbit HK521-XAK Glial Fibrillary Acidic Protein (GFAP) AM020-10M GA-5/Mouse None Glut-1 AM505-10M SPM498/Mouse HK522-XAK Glypican-3 AM539-10M GPC3-88/Mouse HK081-5KE HSA AM550-10M HSA/E8/Mouse HK521-XAK internationalcs@biogenex.com 25 Automated Systems i6000™ Reagents and Consumables Product Name Cat. No. Clone/Source Pre-treatment Heat Shock Protein 27 (HSP 27) AM171-10M G3.1/Mouse HK081-5KE Helicobacter pylori AR442-10RE Polyclonal/Rabbit None HGH (Growth Hormone) AR707-10R Polyclonal/Rabbit HK521-XAK IgM AR427-10R Polyclonal/Rabbit HK051-5KE Inhibin Alpha AM446-10M R1/Mouse HK081-5KE Insulin (New) AN735-10M EP125/Rabbit None Kappa light chain AM048-10M L1C1/Mouse None Kappa light chain AM369-10M K88/Mouse None Ki-67 AM410-10M K2/Mouse HK081-5KE Ki-67 AN527-10M EPR3611/Rabbit HK522-XAK Ki-67 (New) AN727-10M EP5/Rabbit HK522-XAK Ki-67 antigen, Proliferating cell AM297-10M MIB-1/Mouse HK087-5KE Ki-67 antigen, Proliferating cell AM370-10M Ki88/Mouse HK081-5KE Ki67+Lambda Light Chain AC562-10M K-2/Mouse + Polyclonal/Rabbit HK081-5KE Lambda Light Chain (New) AN715-10M EP172/Rabbit HK058-5KE Laminin AR078-10R Polyclonal/Rabbit EK000-5K Lysozyme AR024-10R Polyclonal/Rabbit HK521-XAK Mast Cell Tryptase AM419-10M AA1/Mouse None Melan-A (MART-1) AM361-10M A103/Mouse HK087-5KE HK081-5KE Melanoma AM001-10M HMB45/Mouse Melanoma-Associated Antigen AM077-10M NKI/C3/Mouse None Melanoma gp100 AM536-10M gp100/D5/Mouse HK087-5KE Mesothelin AM433-10M 5B2/Mouse HK058-5K MiTF AM554-10M MiTF/A13/Mouse HK522-XAK MLH1 AM703-10M ES05/Mouse HK081-5KE MMP9 AN504-10M EP1255Y/Rabbit HK522-XAK MSH2 (New) AN743-10M SP46/Rabbit HK521-XAK MSH6 AM454-10M 2D4B5/Mouse HK521-XAK MUC4 AM455-10M 1G8/Mouse HK521-XAK MUC5AC AM456-10M 45M1/Mouse HK521-XAK Mucin 1 (MUC1) AN564-10M EPR1023/Rabbit HK081-5KE Myelin Basic Protein AM380-10M MBP88/Mouse None Myeloperoxidase (MPO) AR496-10R Polyclonal/Rabbit HK522-XAK HK522-XAK Myf4 AM432-10M LO26/Mouse Myosin Heavy Chains, Smooth Muscle AM331-10M SMMS.1/Mouse HK058-5K N-Cadherin AN565-10M EPR1792Y/Rabbit HK081-5KE Napsin A AM701-10M IP64/Mouse HK521-XAK Neurofilament AM073-10M NE-14/Mouse None Neuron Specific Enolase (NSE) AM055-10M MIG-N3/Mouse None NGFR AN522-10M EP1039Y/Rabbit HK522-XAK NGFR (New) AN738-10M EP31/Rabbit HK081-5KE Oct 2 AN558-10M EPR542/Rabbit HK081-5KE Oct 4 (New) AN724-10M EP143/Rabbit HK081-5KE p16 [INK4a] AM540-10M G175-405/Mouse HK081-5KE p16 + KI67 AC601-10M G175-405+EPR3611/Mouse+Rabbit HK081-5KE p27 (Kip1) AN492-10M Y236/Rabbit HK521-XAK P504S (AMACR) AN538-10M RBT-AMACR/Rabbit HK521-XAK P504S (AMACR) AN449-10ME 13H4/Rabbit HK081-5KE p53 (New) AN728-10M EP9/Rabbit HK522-XAK p53 Protein AM195-10M BP53-12-1/Mouse HK087-5KE 26 www.biogenex.com Automated Systems i6000™ Reagents and Consumables Product Name Cat. No. Clone/Source Pre-treatment p53 Protein AM239-10M DO7/Mouse HK087-5KE p53 Protein AM240-10M 1801/Mouse HK087-5KE p63 AM418-10M 4A4/Mouse HK081-5KE p63+CK5 AC602-10M 4A4/Mouse + EPR1600Y/Rabbit HK081-5KE PAP AM532-10M A40010/Mouse HK521-XAK PAX5 AM457-10M ZP007/Mouse HK521-XAK Paxillin (N-Term) AN521-10M Y113/Rabbit HK521-XAK PDCD4 AN524-10M EPR3432/Rabbit HK521-XAK PDGF Receptor-β AN463-10M Y92/Rabbit HK521-XAK PGP9.5 (New) AM736-10ME 3D9/Mouse HK081-5K HK081-5KE PIN4 (p63 + CK HMW + P504S) AM448-10ME 4A4/Mouse+34BE12/Mouse+13H4/Rabbit Placental Alkaline Phosphatase (PLAP) AM228-10M PL8-F6/Mouse None Placental Lactogen (hPL) AR040-10R Polyclonal/Rabbit None PMS2 AN577-10M EPR3947/Rabbit HK081-5KE Progesterone receptor AM172-10ME 1A6/Mouse None Progesterone receptor AN711-10ME EP2/Rabbit HK087-5KE Progesterone receptor (InSite® PR) AM328-10ME PR88/Mouse HK087-5KE Prolactin AM031-10M ME-121/Mouse None Proliferating Cell Nuclear Antigen (PCNA) AM252-10M PC10/Mouse HK087-5KE Prostate specific acid phosphatase (PSAP) AM013-10ME B01-94-21M-NA/Mouse None Prostate specific antigen (PSA) AM014-10ME ErPr-8/Mouse None pS2 Estrogen Inducible Protein AM190-10M PS2.1/Mouse EK002-5K PSMA (New) AN714-10M EP192/Rabbit HK081-5KE RCC (Renal Cell Carcinoma) AM543-10M RCC-26/Mouse HK081-5KE S100 beta (New) AN713-10M EP32/Rabbit HK087-5KE S-100 Protein AR058-10R Polyclonal/Rabbit None S-100 Protein AM058-10M 15E2E2/Mouse None S100P (New) AN712-10M EP186/Rabbit HK087-5KE Sarcomeric Actin AM511-10M ZMSA-5/Mouse HK521-XAK Stat 5-Alpha (C-term) AN480-10M E289/Rabbit HK521-XAK Survivin AN503-10M EP2880Y/Rabbit HK521-XAK Synaptophysin AM363-10M Snp88/Mouse HK081-5KE Tau AM412-10M Tau-2/Mouse HK081-5KE Tau AM459-10M Tau-5/Mouse HK521-XAK Terminal Deoxynucleotidyl Transferase (TdT) AM373-10M TdT/Mouse HK087-5KE TFPU.1 AN520-10M EPR3159Y/Rabbit HK521-XAK Thyroglobulin AM032-10M 2H11/Mouse None Thyroid Transcription Factor (TTF-1) AM397-10M 8G7G3/1/Mouse HK081-5KE TIA-1 AM529-10M 2G9A10F5/Mouse HK521-XAK Topo 2 Alpha AN576-10M EP1102Y/Rabbit HK081-5KE Thyroid Stimulating Hormone (TSH) AM033-10M 5404/Mouse None TTF1+CK5/6 AC563-10M 8G7G3/1/Mouse+EPR1600Y&EPR1602Y/Rabbit HK080-5K TTF1+CK20+CK7 AC605-10M 8G7G3/1+EP1582Y+EPR1619Y/Mouse+Rabbit HK081-5KE TTF1 + GCDFP-15 AC604-10M 8G7G3/1+EP1582Y/Mouse+Rabbit HK087-5KE Tumor-Associated Glycoprotein (TAG-72) AM054-10M B72.3/Mouse None Tyrosinase AM535-10M Ty/G5/Mouse HK521-XAK VEGF AR483-10R Polyclonal/Rabbit HK521-XAK Villin AM525-10M ID2C3/Mouse HK522-XAK Vimentin AM074-10M V9/Mouse None VIP AR530-10R Polyclonal/Rabbit HK522-XAK Wilm’s Tumor AN584-10M CAN-R9(IHC)-56-2/Rabbit HK081-5KE ZAP-70 AM544-10M ZAP70-C3/Mouse HK521-XAK ZAP-70 AN466-10M YE291/Rabbit HK521-XAK internationalcs@biogenex.com 27 Automated Systems i6000™ Reagents and Consumables Detection Systems Our all-inclusive, Super SensitiveTM Detection Systems contain all the reagents required for easy, fast, and exceptional staining. Each kit contains enough reagents to stain approximately 200 slides at 100 µl per slide. The following kit configurations are available to fit the laboratory’s needs for any staining requirement. Reagents are offered in barcoded vials designed for use on the i6000TM and the OptiMax Staining Systems. Product Name Pack size Cat. No. Super Sensitive™ One-step Polymer-HRP Detection Kit/DAB 200 slides QD610-YAXE Super Sensitive™ Polymer HRP Detection System/DAB 200 slides QD410-YAXE Avidin/Biotin Blocking Kit RTU 200 slides HK102-20X Avidin/Biotin Blocking Kit RTU 100 slides HK102-10X Multilink detection kitHRP/DAB (2 components) 200 slides QD470-YCX Note: Please enquire for Double Stain Detection Kits OptiMiser Reagent Vials and Accessories (User Defined) The OptiMiser reagent vials (U.S. & Foreign Equivalent Patents Pending) are available as a 20 ml disposable barcoded pack for use on the i6000TM and the OptiMax staining systems. Product Name Pack size Cat. No. OptiMiser Reagent Vials, Labeled (20 ml) (Empty Vials supplied with 100 corresponding slide barcode labels) 1 each XT026-601 to XT026-799 XT026-601P to XT026-660P OptiMiser Reagent Vials, Unlabeled (20 ml) White Pack of 24 XT026-V24 OptiMiser Reagent Vials, Unlabeled (20 ml) Brown Pack of 24 XT101-24X OptiMiser Universal Vial Holders Pack of 24 XT027-H24 OptiMiser Vial Caps Pack of 24 XT022-CP Empty Reagent Vials for ISH Probes 1 each XT026-PR601 to XT026-PR615 Note: Unlabeled Vials - for open system only 28 www.biogenex.com Automated Systems i6000™ Reagents and Consumables Barrier Slides, PAP Pen, and Barcode Labels OptiPlusTM Positively-charged Barrier Slides (U.S. & Foreign Equivalent Patents Pending) contain hydrophobic barriers that allow the quantity of reagent per slide to be tailored to the size of the specimen. These slides come in three configurations to accommodate different tissue sizes or multiple tissues per slide; A single full-size test area of 25 mm x 40 mm, a single 2/3-size test area of 25 mm x 30 mm, and three 1/3-size test areas per slide, each measuring 25 mm x 15 mm. The permanent hydrophobic barriers are compatible with dewaxing solutions and other reagents. The slides are suitable for use with frozen tissue sections, formalin-fixed paraffin sections, and cytology preparations. Product Name Pack size Cat. No. OptiPlus™ Positively-charged Barrier Slides (full test area) 1 box (72 slides) XT134-SL 1 case (20 boxes) XT134-CL 1 box (72 slides) XT013-SL 1 case (20 boxes) XT013-CL 1 box (72 slides) XT014-SL 1 case (20 boxes) XT014-CL PAP Pen (for 500 - 1000 slides) 1 each XT001-PP Slide Barcode Labels 100/sheet AM6010 to AM7990 AR6010 to AR6600 Product Name Pack size Cat. No. Pipette Tips for i6000™ (1.0 ml) 1 box (192 tips) XT105-01X Pipette Tips for i6000™ (1.0 ml) 5 boxes (960 tips) XT104-05X OptiPlus™ Positively-charged Barrier Slides (2/3 test area) OptiPlus™ Positively-charged Barrier Slides (3 x 1/3 test area) Pipette Tips Each pipette tip is carefully inspected to ensure optimal and accurate performance. Ancillary Reagents EZ-DeWax™ Solutions1 Tissue specimens are usually fixed and embedded in paraffin, sectioned on a microtome, and then attached to slides. Before immunostaining, the sections are traditionally deparaffinized with highly toxic, noxious chemicals (xylene and alcohols or equivalents). BioGenex offers a revolutionary product that simply, easily and rapidly removes the paraffin from mounted tissue slides. Use of nonxylene based BioGenex EZ-DeWaxTM Solution permits a two-step application of a single reagent that completely removes the paraffin, rendering the tissue’s antigenic sites accessible to the antibodies, chromogens and other aqueous solutions. The deparaffinization time is reduced from 45 minutes of manual processing to less than 15 minutes of automated dewaxing on the BioGenex i6000TM Automated Staining System using the EZ-DeWaxTM Solution. The solution simultaneously removes paraffin and rehydrates the tissue. 1 Product Name Pack size Cat. No. EZ-DeWax™ Solution (Concentrated)1 (Requires 500 ml of histologic grade alcohol for reconstitution) 500 ml HK584-5KE US Patent No. 6,632,598; Japanese Patent No. 3532571; European Patent No. 0698118B1. internationalcs@biogenex.com 29 Automated Systems i6000™ Reagents and Consumables Enzymes for Pre-treatment Some tissues require the use of enzymatic pre-treatment before staining to achieve standardized results depending on the antibodies and their different incubation and pre-treatment requirements. Product Name Pack size Cat. No. Pepsin 4-Pack 4 vials of Lyophilized Enzyme Powder, Reconstitution Buffer 4 x 5 ml 200 slides EK000-10K Trypsin 4-Pack 4 vials of Lyophilized Enzyme Powder, Reconstitution Buffer 4 x 5 ml 200 slides EK001-10K Protease XXIV 4-Pack 4 vials of Lyophilized Enzyme Powder, Reconstitution Buffer 4 x 5 ml 200 slides EK002-10K Diastase (Alpha-Amylase Kit) 4 vials of alpha-amylase, 4 vials of alpha-amylase diluent 200 slides EK004-5K Wash Buffers Super SensitiveTM Wash Buffers are used to ensure optimal staining with even spreading of antibodies and other reagents to avoid inconsistent results. Product Name Pack size Cat. No. Super Sensitive™ Wash Buffer, 20X concentrated 500 ml HK583-5KE EZ-AR™ Solutions Product Name Pack size Product Description Cat. No. 1L EZ-AR™ 1 is a Citra based solution. Works at 107°C HK521-XAK EZ-AR™ 2 RTU1 1L EZ-AR™ 2 is a EDTA based solution. Works at 107°C HK522-XAK EZ-AR™ 3 Conc.1 (10X) 500 ml EZ-AR™ 3 is a Citra based solution. Works at 107°C HK543-YOK EZ-AR™ 4 Conc.1 (10X) 500 ml EZ-AR™ 4 is a Tris based solution. Works at 107°C HK544-YOK 1L DeWax solution. Use in combination with other EZ-AR™ solutions HK545-XOK EZ-AR™ 1 RTU 1 1 EZ-AR™ Common, Conc. (5X) Product Name Cat. No. i500 Plus™ LIS Enabled Barcode Label Printer BLS500 Instrument Accessories 1 Product Name Pack size Cat. No. Resin Ribbon 1 Roll XT034-XEX Labels Roll 1 Roll XT035-XBX U.S. Patent Numbers 6,451,551 and 5,578,452 (as well as foreign equivalents) 30 www.biogenex.com Tissue Pre-treatment & Antigen Retrieval Tissue Pre-treatment & Antigen Retrieval EZ-DeWax™ Solutions One-Step DeWaxing and Rehydration Reagent BioGenex EZ-DeWaxTM Solution is a “one-step” product that simultaneously enables the removal of paraffin and allows rehydration of the tissue with a single reagent. In the past, formalin-fixed, paraffin-embedded tissue sections were traditionally deparaffinized with highly toxic, noxious chemicals (i.e. xylene, equivalents). BioGenex, a pioneer in the Immunohistochemistry technology, offers a xylene-free product that removes the paraffin from mounted tissue slides easily and rapidly. Advantages of EZ-DeWaxTM Solutions • • • • • • • • • † Effectively removes paraffin and other wax residues from tissue sections Avoids the use of xylene or xylene substitutes Reduces the use of alcohol in preparing tissue sections for IHC, ISH, H & E, FISH and Special Stains Has no adverse effect on the quality of tissue sections Reduces deparaffinization time from 45 minutes to 10 minutes Produces minimal or no odors Reduces the quantity of toxic solvents used in the lab Minimizes hazardous waste and easy to dispose Optimized for all BioGenex Automated Staining Systems and manual methods Product Name Pack size Cat. No. EZ-DeWax™ Solution (Concentrated) 500 ml HK584-5KE† U.S. Patent No. 6,632,598; U.S. Patent No. 7, 070, 951; Japanese Patent No. 3532571; European Patent No. 0698118B1 . 32 www.biogenex.com Tissue Pre-treatment & Antigen Retrieval Antigen Retrieval Method BioGenex is the inventor of Antigen Retrievala enabling technology. Antigen Retrieval is an effective way of unmasking antigenic epitopes on the surface of formalin-fixed, parraffin-embedded tissue sections using microwave heating. Covered by exclusive patents1 issued to BioGenex, this method to recover antigenicity in formalin-fixed, paraffin-embedded tissue sections has been routinely practiced in laboratories throughout the world. The Antigen Retrieval technique breaks the formalin induced cross-linking bonds between epitopes and unrelated proteins, thereby allowing better penetration of antibody and accessibility of epitopes. Advantages of the Antigen Retrieval Technique • • • • • Enhanced exposure of antigenic epitopes on the surface of the tissue section Reduces time for primary antibodies incubation Consistent and reliable staining quality Eliminates false negative staining results in FFPE tissue sections Ease-of-use Different antibodies require different conditions for Antigen Retrieval. BioGenex offers several types of Antigen Retrieval solutions: 1. 2. 3. 4. 5. 6. EZ-ARTM Solutions Citra Citra Plus AR-10† H&E Retrieval DeCal Tonsil tissue stained with anti-Ki-67 antibody using AEC chromogen without antigen retrieval Tonsil tissue stained with anti-Ki-67 antibody using AEC chromogen with antigen retrieval a Shi S-R, Key ME, Kalra KL. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 39:741-8, 1991. 1 U. S. Patent Numbers 5,244,787; 5,578,452 and 6,451,551; Japanese Patent No. 3108099; European Patent No. 0607422. internationalcs@biogenex.com 33 Tissue Pre-treatment & Antigen Retrieval Antigen Retrieval Accessory Kit The Antigen Retrieval Accessory Kit consists of slide holders and slide baths that make it convenient and compatible with any of the several Antigen Retrieval solutions. To accommodate microwave heating, the slide baths and slide holders are made of heat-stable thermoplastic polyolefin and hydrocarbon polymers of acetal resins. These accessories may be used in a microwave or a pressure cooker. Antigen Retrieval Accessory Kits Product Name* Pack size Cat. No. 3 Slide Baths with Lids 3 Slide Holders (72-slide capacity) 1 Kit MW001-HB 1 Slide Bath with Lid 1 Slide Holder (24-slide capacity) 1 Kit MW001-SU EZ-AR™ Elegance Solutions The EZ-AR™ Elegance Solutions are designed to enable optimal dewaxing, rehydration and antigen retrieval on FFPE sections. The solutions can reach temperature of 107°C without boiling thus preventing reagent evaporation. When used with EZ-Retriever® System or Xmatrx® Automated Staining System, these solutions ensure superior reproducible results with uncompromised staining quality. Features and Benefits Works at 107°C without boiling • Minimizes reagent evaporation • Enhances exposure of antigenic epitopes Eliminates enzymatic pre-treatment • Reduces background • Preserves tissue morphology Reusable up to three times • Reduces material cost Non-hazardous, non-flammable and odorless • Safe and eco-friendly Product Name Pack Size Product Description Cat. No. EZ-AR™ 1 Elegance RTU 1L EZ-AR™ 1 Elegance is Citra based solution. Works at 107°C HK546-XAK EZ-AR™ 2 Elegance RTU 1L EZ-AR™ 2 Elegance is EDTA based solution. Works at 107°C HK547-XAK For EZ-AR™ Elegance Solutions compatible with Xmatrx® Automated Staining System, refer to Automation Section 34 www.biogenex.com Tissue Pre-treatment & Antigen Retrieval EZ-AR™ Solutions Enable Highly Reproducible and Superior Quality Stains The EZ-ARTM Solutions possess unique properties that enable optimal dewaxing, rehydration and antigen retrieval in formalin-fixed, paraffin-embedded tissue sections. Some solutions can reach temperatures as high as 95oC. These solutions when used with EZ-Retriever® System facilitate the production of highly reproducible and superior quality stains in a considerably short time without compromising the morphology and antigenicity of the tissue. Features and Benefits Unique superheating properties • Increases the availability of antigenic epitopes in tissues Eliminates enzymatic pre-treatment of tissue sections • Reduces background staining • Preserves tissue morphology Streamlined set of solutions • Offers a simple solution to antigen retrieval pre-treatment needs Short, simple, and standardized protocols for all BioGenex antibodies • Reduces tissue pre-treatment time • Eliminates guesswork in optimizing protocol Fast uniform heating and cooling of solutions • Reduces tissue pre-treatment time Non-hazardous, non-flammable and odorless • Safe and Eco-friendly Product Name Pack Size Product Description Cat. No. EZ-AR™ 1 RTU 1L EZ-AR™ 1 is a Citra based solution. Works at 95°C HK521-XAK EZ-AR™ 2 RTU 1L EZ-AR™ 2 is a EDTA based solution. Works at 95°C HK522-XAK EZ-AR™ Common, Conc. (5X) 1L DeWax solution. Use in combination with other EZ-AR™ solutions HK545-XOK EZ-AR™ 3 Conc. (10X) 500 ml EZ-AR™ 3 is a Citra based solution. Works at 95°C HK543-YOK EZ-AR™ 4 Conc. (10X) 500 ml EZ-AR™ 4 is a Tris based solution. Works at 95°C HK544-YOK For Laboratory Use Only internationalcs@biogenex.com 35 Tissue Pre-treatment & Antigen Retrieval Antigen Retrieval Solutions Citra† Solutions This citrate-based solution provides excellent staining of routine paraffin sections with most BioGenex antibodies, including Progesterone Receptor (PR), p53 protein, and Ki-67 Antigen (clone Ki88) when used in a microwave. • Retrieves most antigens in formalin-fixed, paraffin-embedded tissue sections • Excellent results with difficult antigens like PR, p53 and Ki-67 Antigen Product Name Pack size Cat. No. Antigen Retrieval Citra Solution (10X Concentrated) 100 ml HK086-5K Antigen Retrieval Citra Solution (10X Concentrated) 500 ml HK086-9K Antigen Retrieval Citra Solution (Ready-to-use) 250 ml HK087-5KE Antigen Retrieval Citra Solution (Ready-to-use) 1000 ml HK087-20KE Please Contact Technical Support for a complete list of recommended antibodies to be used with Antigen Retrieval Citra Solution. Citra Plus† Solutions This product is an enhanced version of our standard Antigen Retrieval Citra Solution for use in microwave and is designed for immunostaining with Estrogen Receptor (ER), HSP27, and certain other antibodies as recommended by BioGenex. • Retrieves most antigens in formalin-fixed, paraffin-embedded tissues • Excellent results with ER Antigen Product Name Pack size Cat. No. Antigen Retrieval Citra Plus Solution (10X Concentrated) 100 ml HK080-5K Antigen Retrieval Citra Plus Solution (10X Concentrated) 500 ml HK080-9K Antigen Retrieval Citra Plus Solution (Ready-to-use) 250 ml HK081-5KE Antigen Retrieval Citra Plus Solution (Ready-to-use) 1000 ml HK081-20KE Please Contact Technical Support for a complete list of recommended antibodies to be used with Antigen Retrieval Citra Plus† Solution. Breast Carcinoma tissue stained with Anti-Progesterone Receptor [PR88] MAb, using AEC chromogen without Antigen Retrieval. † Breast Carcinoma tissue stained with Anti-Progesterone Receptor [PR88] MAb, using AEC chromogen with Antigen Retrieval using Citra. Breast Carcinoma tissue stained with Anti-Estrogen Receptor [ER88] MAb, using DAB chromogen without Antigen Retrieval. Breast Carcinoma tissue stained with Anti-Estrogen Receptor [ER88] MAb, using DAB chromogen with Antigen Retrieval using Citra Plus. U.S. Patent No. 5,578,452; Japanese Patent No. 3108099; European Patent No. 0607422. 36 www.biogenex.com Tissue Pre-treatment & Antigen Retrieval † AR-10 Solutions Antigen Retrieval AR-10† is a Tris-based solution that produces excellent immunostaining with BioGenex antibodies like Caldesmon, CD3 (Clone PS1), c-myc Protein, PECAM (Clone 9G11), GLEPP1, and other antigens in formalin-fixed, paraffin embedded tissue sections when used in a microwave. Product Name Pack size Cat. No. Antigen Retrieval AR-10 Solution (10X Concentrated) 100 ml HK057-5KE Antigen Retrieval AR-10 Solution (Ready-to-use) 250 ml HK058-5K Antigen Retrieval AR-10 Solution (Ready-to-use) 1000 ml HK058-20K Please Contact Technical Support for a complete list of recommended antibodies to be used with Antigen Retrieval AR-10† Solution. Tonsil tissue stained with anti-CD3 MAb using DAB chromogen without Antigen Retrieval using AR-10†. † Tonsil tissue stained with anti-CD3 MAb using DAB chromogen with Antigen Retrieval using AR-10†. U.S. Patent No. 5,578,452; Japanese Patent No. 3108099; European Patent No. 0607422. H&E Retrieval Solutions The H&E Retrieval Solution will help recover nuclear staining in burnt or overfixed formalin-fixed, paraffin-embedded tissue sectionsa . • • • Proven to restore nuclear staining detail for improved hematoxylin staining in burnt tissues and fixed or overfixed formalin-fixed, paraffin-embedded tissue sections Highly recommended pre-treatment step for specimens dried out before fixing in formalin, over-DeCal specimens (e.g. bone marrow biopsies) and over-processed specimens (i.e. fragile specimens such as needle biopsies) H&E Retrieval solution can be used at room temperature for some frozen tissue sections & specimens that may contain freezing artifacts Product Name Pack size Cat. No. H&E Retrieval Solution (10X Concentrated) 100 ml HK169-5K Burnt kidney tissue stained after standard pre-treatment. internationalcs@biogenex.com Nuclear data restored by microwave heating in H&E Solution. 37 Tissue Pre-treatment & Antigen Retrieval Enzymes for Tissue Digestion Some tissues require the use of enzymatic pre-treatment before staining to achieve standardized results depending on the antibodies and their different incubation and pre-treatment requirements. Each kit contains three vials of lyophilized enzyme powder and 15 ml of reconstitution buffer, enabling you to make fresh enzyme solutions as needed. The Trypsin 3-Pack and Pepsin 3-Pack contain well-established enzymes suitable for routine pre-treatment at 37°C. The Protease XXIV 3-Pack contains a universal digestive agent that allows for fast and effective pre-treatment at room temperature. Enzymes for Tissue Digestion Product Name Pack size Cat. No. Pepsin 3-Pack 3 vials of lyophilized enzyme powder, 3 x 5 ml reconstitution buffer 150 slides EK000-5K Trypsin 3-Pack 3 vials of lyophilized enzyme powder, 3 x 5 ml reconstitution buffer 150 slides EK001-5K Protease XXIV 3-Pack 3 vials of lyophilized enzyme powder, 3 x 5 ml reconstitution buffer 150 slides EK002-5K Diastase (Alpha-Amylase Kit) 4 vials of alpha-amylase, 4 vials of alpha-amylase diluent 200 slides EK004-5K Leiomyoma tissue stained with Factor VIII MAb using AEC chromogen following Pepsin Pre-treatment. Colonic adeno carcinoma stained with Anti-Cytokeratin 20 MAb following Protease Pre-treatment. Squamous Carcinoma tissue stained with AntiCytokeratin (High Molecular Weight) MAb using AEC chromogen following Trypsin Pre-treatment. With the new paradigm in Antigen Retrieval using the EZ-Retriever® System, enzymatic pre-treatment can be eliminated from the protocol. For more information on the EZ-Retriever® System please refer to the Automation section. 38 www.biogenex.com Tissue Pre-treatment & Antigen Retrieval NordicWare Microwave Tender Cooker ® Placing the NordicWare® Microwave Tender Cookera within a microwave is an effective method for enhancing staining with the Antigen Retrieval technique. The heat produced under enhanced pressure can reduce the build up of gas bubbles on the surface of tissues. This improves the intensity of staining, accompanied by preservation of tissue and cell morphology. This pressure cooker is also optimized for use with various BioGenex Antigen Retrieval solutions. Product Name Pack size Cat. No. NordicWare® Microwave Tender Cooker 1 Unit NW001-PC For Laboratory Use only a NordicWare® is a registered trademark of NordicWare Corp. internationalcs@biogenex.com 39 Tissue Pre-treatment & Antigen Retrieval 40 www.biogenex.com Primary Antibodies Primary Antibodies Primary Antibodies BioGenex antibodies are optimized to provide maximum signal with minimum background when used for immunohistochemical staining. Format Ready-to-use antibodies are fully optimized for use with BioGenex detection systems without the need for further dilution or titration. Readyto-use, Super SensitiveTM antibodies are fully quality controlled. These antibodies are recommended for use with all Super SensitiveTM Detection Systems to provide optimum staining. The ready-to-use antibody formats are indicated by catalog numbers prefixed with AC (Antibody Cocktails), AM (Mouse Monoclonal Antibodies), AN (Rabbit Monoclonal Antibodies), AY&AX (Monoclonal Antibodies for Xmatrx®), AW (Polyclonal Antibodies for Xmatrx® ) and AR (Polyclonal Antibodies). Concentrated antibodies are provided with recommended dilutions for optimal use with BioGenex detection systems, allowing rapid titration and testing. These provide a more economical alternative for laboratories doing high volume immunostaining. The concentrated antibody formats are indicated by catalog numbers prefixed with MU (Mouse Monoclonal Antibodies), NU (Rabbit Monoclonal Antibodies) and PU (Polyclonal Antibodies). All BioGenex concentrated antibodies are thoroughly tested for immunostaining applications and come with recommended dilutions for use with BioGenex detection systems. For specific information on individual antibody titers, please call BioGenex Technical Support at 1(800)421-4149 or write to: support@biogenex.com. Pack Size Unless otherwise specified, the following table lists the pack size for the available formats of antibodies: Description Pack size Order information in Cat. No. Ready-to-use (Manual) 3 ml 6 ml 2M and 2ME -5M, 5ME, 5R and 5RE Ready-to-use (i6000TM) 10 ml 10M, 10ME, 10R and 10RE Ready-to-use (Xmatrx ) 16 ml -YCD and YCDE Concentrated 1 ml UC, UCE, UP and UPE ® Tissue Type Unless otherwise noted, all primary antibodies are optimized for use on routine formalin-fixed paraffin-embedded tissue. Optimization All BioGenex primary antibodies are quality controlled and tested to provide optimum immunohistochemical staining when used with the appropriate BioGenex detection system. The correct optimization of antibody and detection system minimizes the potential for false negative or false positive staining. Recommended Pre-treatment The recommended pre-treatment for each antibody is provided under each description of the antibody. BioGenex offers EZ-Retriever® System for Dewaxing, Rehydration and Antigen Retrieval, that streamlines and simplifies tissue pretreatment. For more details on the system please refer to Automated Systems section. We recommend that you refer to the datasheet (i.e. package insert) provided with the antibodies for up-to-date information on the pretreatment conditions or please contact BioGenex Technical Support at 1(800)421-4149 or write to: support@biogenex.com. Positive Tissue Control Slides And Barrier Slides BioGenex provides positive tissue control for use with the antibodies. The appropriate catalog number for the positive control slides with and without barrier are provided. For further details, refer to the Tissue Control section. Antibody Look-Up Table The table titled as “Antibody Look-Up Table” in the beginning of this section provides comprehensive information on all BioGenex primary antibodies along with positive controls. IVD Products Unless specified otherwise, all Primary Antibodies listed in this Section are for In Vitro Diagnostic Use. 42 www.biogenex.com Primary Antibodies Antibody Look-up Table Antibody Clone (Species) ‡ Localization Positive Control Regulatory Classification Page Aberrant Endothelial Cell 4A11(M) Cyt Tonsil / Lymph Node IVD 51 ACTH AH26(M) Cyt Pituitary IVD 51 Actin, Muscle-Specific HHF35(M) Cyt Heart / Skeleton Muscle IVD 51 Actin, Smooth Muscle 1A4(M) Cyt Colon IVD 51 Adenovirus A62020069P(M) Nuc and Cyt (Frozen) Adenovirus infected cells IVD 52 Alpha-1-Antichymotrypsin α1A88(M) Cyt Liver carcinoma IVD 52 Alpha-1-Antitrypsin Polyclonal(R) Cyt Hepatocellular carcinoma IVD 52 Alpha-Actinin JLN20(M) Cyt Muscle IVD 52 IVD 53 Alpha-Fetoprotein (AFP) C3(M) Cyt Hepatocellular carcinoma Alpha-Tubulin DM-1A(M) Cyt Skin/Lung IVD 53 Androgen Receptor F39.4.1(M) Nuc Prostate/Prostate Ca. IVD 53 Apolipoprotein E EP1373Y(R) Mem and Cyt Liver IVD 53 B Cell MB2(M) Cyt Tonsil IVD 54 B Lymphocyte Antigen 36 (BLA.36) A27-42(M) Mem B Lyphocytes/Hodgkin IVD 54 Bax Protein Polyclonal(R) Cyt and Mem Breast Carcinoma IVD 54 Bcl-2 E17(R) Cyt Follicular and lymphoma IVD 54 Bcl-2 EP36(R) Cyt Tonsil IVD 55 Bcl-2 Oncoprotein Bcl-2/100(M) Cyt Tonsil IVD 55 Bcl-6 LN22(M) Nuc Tonsil IVD 55 Bcl-x E18(R) Mem Breast Carcinoma IVD 55 Beta-Catenin E247(R) Mem/Cyt/Nuc Colon Carcinoma IVD 56 Beta-Tubulin DM-1B(M) Cyt Skin/Lung IVD 56 Beta-Tubulin II JDR3B8(M) Cyt Colon IVD 56 Beta-Tubulin III SDL3D10(M) Cyt Heart IVD 56 Beta-Tubulin IV ONS1A6(M) Cyt Lung IVD 57 bFGF (Basic Fibroblast Growth Factor) bFGF88(M) Cyt Adenocarcinoma IVD 57 Blood Group Antigen Lewis A 7LE(M) Cyt and Mem Stomach IVD 57 Blood Group Antigen Lewis B 2-25LE(M) Cyt and Mem Stomach IVD 57 BOB-1 SP92(R) Nuc Tonsil IVD 58 BRCA1 Protein Polyclonal(R) Nuc and Cyt Breast Carcinoma IVD 58 Breast Cancer Antigen BCA 225 CU18(M) Cyt Breast Carcinoma IVD 58 CA 19-9 C241:5:1:4(M) Cyt Colon IVD 58 CA 125 (Ovarian Tumor Marker) Ov185:1(M) Mem/Cyt Ovary Carcinoma IVD 59 Caldesmon E89(R) Cyt Prostate IVD 59 Caldesmon,High MW,Smooth muscle h-CD(M) Cyt Breast Carcinoma IVD 59 Calponin CALP(M) Cyt Breast Carcinoma IVD 59 Calponin-1 (C-term) EP798Y(R) Cyt Pleomorphic Adenoma IVD 60 Calretinin 2 E7(M) Cyt Brain Tissue IVD 60 Calretinin Polyclonal(R) Cyt Brain IVD 60 Carcinoembryonic Antigen (CEA) B01-94-11M-P(M) Cyt Normal Colon/ Colon Carcinoma IVD 60 Carcinoembryonic Antigen (CEA) CEA88(M) Cyt Bowel Carcinoma IVD 61 Carcinoembryonic Antigen (CEA) Polyclonal(R) Cyt Bowel/Colon Carcinoma IVD 61 Please consult the data sheet for pre-treatment and protocol information. Unless specified otherwise, all primary antibodies listed in this table are for FFPE tissue specimens ‡ M: Mouse; R:Rabbit internationalcs@biogenex.com 43 Primary Antibodies Antibody Clone (Species) ‡ Localization Positive Control Regulatory Classification Page Catenin (p120) YE372(R) Cyt/Mem Breast Carcinoma IVD 61 Catenin Delta 1 (p120) Polyclonal(R) Cyt and Mem Breast Carcinoma IVD 61 Cathepsin D C15(M) Cyt Breast Carcinoma IVD 62 CD1a O10(M) Mem Lymph Node IVD 62 CD2 AB75(M) Mem Lymphoma IVD 62 CD3 Epsilon EP449E(R) Mem Spleen IVD 62 CD3 (T Cell) UCHT1(M) Mem(Frozen) Tonsil IVD 63 CD3 (T Cell) PS1(M) Mem Tonsil IVD 63 CD4 4B12(M) Mem Tonsil IVD 63 CD4 EP204(R) Mem Tonsil/T-cell lymphoma IVD 63 CD5 4C7(M) Mem Tonsil IVD 64 CD5 EP2952(R) Mem Lymph Node IVD 64 CD7 LP15(M) Mem Tonsil IVD 64 CD8 1A5(M) Mem Tonsil IVD 64 CD8 SP16(R) Mem Tonsil IVD 65 CD8 (C-Term) EP1150Y(R) Membrane Tonsil IVD 65 CD8 (T Cell,Suppressor/Cytotoxic) T8(M) Mem(Frozen) Tonsil IVD 65 CD10 56C6(M) Mem Lymphoma IVD 65 CD11b (C3bi Receptor) M01(M) Mem(Frozen) Tonsil IVD 66 CD11b/ITAM EPR1344(R) Mem Spleen IVD 66 CD11C EP1347Y(R) Mem Tonsil Tissue IVD 66 CD14 EPR3653(R) Mem Placenta IVD 66 CD15 (Blood group antigen Lewis X) BRA4F1(M) Mem/Cyt/ perinuclear structures Bone Marrow/Hodgkins IVD 67 CD16 2H7(M) Mem and Cyt Lymph Node/Breast IVD 67 CD19 EP169(R) Mem Tonsil IVD 67 CD20 CD20/C23(M) Membrane Spleen/Tonsil IVD 67 CD20 (B Cell) L-26(M) Mem Tonsil IVD 68 CD20 (B Cell) B1(M) Mem(Frozen) Tonsil IVD 68 CD21 EP3093(R) Mem Tonsil IVD 68 CD21 (B Cell) B2(M) Mem(Frozen) Tonsil/Lymph Node IVD 68 CD22 FPC1(M) Mem Tonsil IVD 69 CD23 Polyclonal(R) Mem Tonsil IVD 69 CD25 4C9(M) Mem Tonsil IVD 69 CD29 JB1a(M) Mem Breast IVD 69 CD30 (Ki-1 Antigen) Ber-H2(M) Mem and Cyt Hodgkin’s Lymphoma IVD 70 CD30 (Ki-1 Antigen) HRS-4(M) Mem(mostly perinuclear dot positivity) and cyt Hodgkin’s Lymphoma IVD 70 CD31 (Endothelial Cell) JC/70A(M) Mem and Cyt Colon Carcinoma IVD 70 CD31 (PECAM-1) 9G11(M) Mem and Cyt Lymph node IVD 70 CD34 (Endothelial Cell) QBend/10(M) Mem Adenocarcinoma lung IVD 71 CD35 RLB25(M) Mem Tonsil IVD 71 CD35 SP191(R) Mem Tonsil IVD 71 CD41/Integrin Alpha IIb EP178(R) Mem/Cyt Spleen IVD 71 CD43 & CD45RA Cocktail MT1 &MB1(M) Mem Tonsil IVD 72 CD43 (T Cell,Leukosialin) DFT-1(M) Mem Tonsil IVD 72 Please consult the data sheet for pre-treatment and protocol information. Unless specified otherwise, all primary antibodies listed in this table are for FFPE tissue specimens ‡ M: Mouse; R:Rabbit 44 www.biogenex.com Primary Antibodies Antibody Clone (Species) ‡ Localization Positive Control Regulatory Classification Page CD44 (Phagocytic Glycoprotein-1,HCAM) DF1485(M) Mem Tonsil IVD 72 CD45 (Leukocyte common Antigen,LCA) PD7/26/16&2B11(M) Mem Tonsil IVD 72 CD45 (Leukocyte common Antigen,LCA) LJ27.9(M) Mem Tonsil IVD 73 CD45 Cocktail (Leukocyte Antigen, LCA) MEM55+L J 27.9(M) Mem Tonsil IVD 73 CD45RA (B Cell) MB1(M) Mem Tonsil IVD 73 CD45RB MEM55(M) Mem Tonsil IVD 73 CD45RC (T Cell) MT2(M) Mem Tonsil IVD 74 CD45RO (T Cell) UCHL-1(M) Mem Tonsil IVD 74 CD48 EP148(R) Mem Tonsil IVD 74 CD53 EP179(R) Mem Tonsil IVD 74 CD56 (Natural Killer Cell,NCAM) NKH-1(M) Mem(Frozen) Tonsil IVD 75 CD57 (Natural Killer Cell) NK-1(M) Mem and Cyt Tonsil IVD 75 CD61 EP2417Y(R) Mem Kidney IVD 75 CD63 EP211(R) Cyt/Mem Melanoma/Prostate Ca. IVD 75 CD66 BY114(M) Mem Tonsil IVD 76 CD68 KP1(M) Cyt Stomach/Lymph node IVD 76 CD68 CD68/G2(M) Cyt Histiocytoma IVD 76 CD71 (Transferrin Receptor) T9(M) Mem(Frozen) Tonsil IVD 76 CD71 (Transferrin Receptor) H68.4(M) Mem and Cyt Bone Marrow/Fetal Liver IVD 77 CD74 (B Cell) LN2(M) Mem and Cyt Tonsil IVD 77 CD79a 11E 3(M) Mem Lymph Node/Tonsil IVD 77 CD79a EP82(R) Mem/Cyt Tonsil/B-cell lymphoma IVD 77 CD90 EP56(R) Mem/Cyt Thymus IVD 78 CD95 EP208(R) Mem/Cyt Tonsil IVD 78 CD99 EPR3097Y(R) Mem Ewings Sarcoma IVD 78 CD99 (MIC2 Antigen) HO36.1.1(M) Mem Thymus/Tonsil IVD 78 CD103 EP206(R) Mem Colon IVD 79 CD105 4G11(M) Mem Uterus IVD 79 CD117 T595(M) Mem and Cyt Gastrointestinal stromal tumor/Stomach IVD 79 CD117 YR145(R) Mem and Cyt Gastrointestinal stromal tumor/Stomach IVD 79 CD146 EP54(R) Cyt/Mem Placenta IVD 80 CD205 EP176(R) Mem/Cyt Tonsil IVD 80 CD227 (MUCIN 1) VU-4H5(M) Cyt Colon/Mucinous Adenocarcinoma IVD 80 CDw75 (B Cell) LN1(M) Mem and Cyt Tonsil IVD 80 CDX-2 CDX2-88(M) Nuc Colon IVD 81 c-erbB-2 (HER-2/neu) CB11(M) Mem Breast Carcinoma IVD 81 c-erbB-2 (HER-2/neu) EP3(R) Mem Breast Carcinoma RUO 81 c-erbB-3 (HER-3) RTJ1/A2(M) Mem Breast Carcinoma IVD 81 Chromogranin A LK2H10(M) Cyt Pancreas IVD 82 Chromogranin A PHE-5(M) Cyt Pancreas IVD 82 IVD 82 Claudin 1 Polyclonal(R) Mem/Cell junction Breast Carcinoma or Spleen Claudin-5 EP224(R) Cell junction/Mem Vascular/Lung Squamous Ca. IVD 82 c-myc Protein 9E 10(M) Nuc Adenocarcinoma IVD 83 Please consult the data sheet for pre-treatment and protocol information. Unless specified otherwise, all primary antibodies listed in this table are for FFPE tissue specimens ‡ M: Mouse; R:Rabbit internationalcs@biogenex.com 45 Primary Antibodies Regulatory Classification Page Extracellular matrix Skin IVD 83 Basal laminae/Cyt Skin IVD 83 IVD 83 Antibody Clone (Species) ‡ Localization Collagen III HWD1.1(M) Collagen IV COL-94(M) Positive Control Cyclin D1 Polyclonal(R) Nuc Thyroid Carcinoma/ Breast Carcinoma) Cyclin D1 EPR2241(IHC)-32(R) Nuc Thyroid Carcinoma IVD 84 Cyclin E2 EP454Y(R) Nuc Glioblastoma IVD 84 Cytokeratin 4 EP4(R) Cyt Esophagus IVD 84 Cytokeratin 4 6B10(M) Cyt Tonsil RUO 84 IVD 85 Cytokeratin 5 EPR1600Y(R) Cyt Squamous Cell Carcinoma Cytokeratin 5 & 6 EPR1600Y & EPR1602Y(R) Cyt Squamous Cell Carcinoma IVD 85 Cytokeratin 5 & Cytokeratin 14 EP24/EP61(R) Cyt Prostate IVD 85 IVD 85 Cytokeratin 6 EPR1602Y(R) Cyt Squamous Cell Carcinoma Cytokeratin 7 OV-TL12/30 Cyt Adenocarcinoma IVD 86 Cytokeratin 7 EPR1619Y(R) Cyt Breast Cancer IVD 86 Cytokeratin 7 & 8 OV-TL12/30 & C51(M) Cyt Breast Carcinoma IVD 86 Cytokeratin 8 C-51(M) Cyt Breast Carcinoma IVD 86 Cytokeratin 8 & 18 5D3(M) Cyt Colon Carcinoma IVD 87 Cytokeratin 10 DEK-10(M) Cyt Skin IVD 87 IVD 87 Cytokeratin 13 AE8(M) Cyt Tongue/Esophagus/ Tonsil Cytokeratin 14 LL002(M) Cyt Squamous Cell Carcinoma IVD 87 Cytokeratin 15 EPR1614Y(R) Cyt Squamous Cell Carcinoma IVD 88 Cytokeratin 16 EP1615Y(R) Cyt Squamous Cell Carcinoma IVD 88 Cytokeratin 17 E27(M) Cyt Basal Cell carcinoma and squamous cell carcinoma tissue IVD 88 Cytokeratin 18 DC-10(M) Cyt Breast Carcinoma IVD 88 Cytokeratin 19 RCK108(M) Cyt Colon Carcinoma IVD 89 Cytokeratin 20 IT-Ks20.8(M) Cyt Colon Carcinoma IVD 89 Cytokeratin 20 EPR1622Y(R) Cyt Colon Carcinoma IVD 89 Cytokeratin Cocktail AE1 and AE3(M) Cyt Skin IVD 89 Cytokeratin Cocktail, Broad Spectrum 34ßE12/C51/AE1(M) Cyt Skin IVD 90 Cytokeratin Cocktail, Broad Spectrum LL002+DEK10+RCK108+ OV-TL12/30+C11(M) Cyt Breast Carcinoma IVD 90 Cytokeratin, High MW 34ßE12(M) Cyt Prostate IVD 90 IVD 90 Cytokeratin, High MW (Basic) AE3(M) Cyt Squamous Cell Carcinoma Cytokeratin, Low MW AE1(M) Cyt Adenocarcinoma IVD 91 Cytokeratin, Pan Lu-5(M) Cyt Colon Carcinoma IVD 91 Cytokeratin, Pan C11(M) Cyt Breast Carcinoma IVD 91 Cytomegalovirus (CMV) BM204(M) Nuc Lung RUO 91 Desmin D33(M) Cyt Leiomyoma IVD 92 DOG1 1.1(M) Cyt/Mem GIST Tissues IVD 92 Dystrophin Dys1(Dy4/6D3)(M) Mem Muscle IVD 92 Please consult the data sheet for pre-treatment and protocol information. Unless specified otherwise, all primary antibodies listed in this table are for FFPE tissue specimens ‡ M: Mouse; R:Rabbit 46 www.biogenex.com Primary Antibodies Antibody Clone (Species) ‡ Localization Positive Control Regulatory Classification Page Dystrophin Dys2(Dy8/6C5)(M) Mem Muscle IVD 92 E-Cadherin 36(M) Mem Breast Carcinoma/Colon Carcinoma IVD 93 E-Cadherin EP6(R) Mem Colon IVD 93 EGFR Polyclonal(R) Mem Squamous Carcinoma IVD 93 EGFR(PAN) EP38Y(R) Mem Squamous Carcinoma IVD 93 Ep-CAM E144(R) Mem Adenoma IVD 94 Epithelial Membrane Antigen (EMA) E29(M) Mem & Cyt Lung IVD 94 IVD 94 Epithelial Membrane Antigen (EMA) Mc5(M) Mem & Cyt Sebaceous Carcinoma/ Breast cancer Epithelial-Specific Antigen MOC-31(M) Mem Colon Carcinoma IVD 94 IVD 95 Epstein-Barr Virus Early Antigen 1108-1(M) Nuc/Cyt EBV Infected culture cells erbB-2 (HER-2/neu) EP1045Y(R) Mem Breast Carcinoma IVD 95 Estradiol Polyclonal(R) Nuc Breast Carcinoma IVD 95 Estrogen Receptor 1D5(M) Nuc Breast Carcinoma IVD 95 Estrogen Receptor EP1(R) Nuc Breast Carcinoma IVD 96 Estrogen Receptor (ER Alpha) EPR703(2)(R) Nuc Breast Carcinoma IVD 96 Estrogen Receptor (InSite® ER) ER88(M) Nuc Breast Carcinoma IVD 96 Estrogen Receptor-Beta (ER-β) Polyclonal(R) Nuc Breast Carcinoma IVD 96 Factor VIII-Related antigen F8 2.2.9(M) Cyt Leiomyoma IVD 97 Factor XIII Subunit A E980.1(M) Cyt Placenta IVD 97 Factor XIII A EP3372(R) Cyt Bone Marrow IVD 97 Fascin FCN01(M) Cyt Lymph Node IVD 97 Fibronectin F14(R) Cyt and extracellular matrix Placental tissue IVD 98 Follicle Stimulating Hormone (FSH) Polyclonal(R) Cyt Pituitary IVD 98 Gastrin Polyclonal(R) Cyt Stomach IVD 98 GCDFP-15 EP1582Y(R) Cyt Breast Carcinoma IVD 98 Glial Fibrillary Acidic Protein (GFAP) GA-5(M) Cyt Cerebellum IVD 99 Glial Fibrillary Acidic Protein (GFAP) Polyclonal(R) Cyt Cerebellum IVD 99 Glomerular Epithelial Protein 1 (GLEPP-1) 5C11(M) Mem Kidney IVD 99 Glucagon Polyclonal(R) Cyt Pancreas IVD 99 IVD 100 Glut-1 SPM498(M) Mem/Cyt Esophagus Carcinoma/ Squamous Carcinoma Glutathione S-Transferase Pi (GST Pi) Polyclonal(R) Nuc & Cyt Breast IVD 100 RUO 100 Glypican-3 (GPC3) GPC3-88(M) Cyt/Mem Hepatocellular carcinoma Granulocyte BM-2(M) Cyt Hodgkin IVD 100 Growth Hormone (Hgh) Polyclonal(R) Cyt Pituitary IVD 101 Heat Shock Protein (HSP-70) BRM-22(M) Cyt Breast Carcinoma IVD 101 Heat Shock Protein 27 (HSP 27) G3.1(M) Cyt Breast Carcinoma IVD 101 Helicobacter pylori Polyclonal(R) Bacterium Stomach IVD 101 Hemoglobin A Polyclonal(R) Cyt Placenta IVD 102 Hepatitis B Virus Core Antigen (HBcAg) Polyclonal(R) Nuc Liver RUO 102 Herpes Simplex Virus Type I (HSV I) Polyclonal(R) Nuc/Cyt Lung IVD 102 Herpes Simplex Virus Type II (HSV II) Polyclonal(R) Nuc/Cyt Lung IVD 102 HLA-DR LN3(M) Mem Tonsil RUO 103 Please consult the data sheet for pre-treatment and protocol information. Unless specified otherwise, all primary antibodies listed in this table are for FFPE tissue specimens ‡ M: Mouse; R:Rabbit internationalcs@biogenex.com 47 Primary Antibodies Antibody Clone (Species) ‡ Localization Positive Control Regulatory Classification Page HSA HSA/E8(M) Cyt Liver Tissues IVD 103 Human Chorionic Gonadotropin (hCG) Beta M94138(M) Cyt Placenta IVD 103 IgA Polyclonal(R) Mem & Cyt Tonsil IVD 103 IgD Polyclonal(R) Mem & Cyt Tonsil IVD 104 IgG IgG88(R) Mem & Cyt Tonsil IVD 104 IgG Polyclonal(R) Mem & Cyt Tonsil IVD 104 IgM IgM88(M) Mem & Cyt Tonsil IVD 104 IgM Polyclonal(R) Mem & Cyt Tonsil IVD 105 Inhibin-Alpha R1(M) Cyt Ovary IVD 105 Insulin HB125(M) Cyt Pancreas IVD 105 Insulin EP125(R) Cyt Pancreas/Insulinoma IVD 105 J Chain JC88(M) Cyt Tonsil IVD 106 Kappa Light Chain L1C1(M) Cyt Tonsil IVD 106 Kappa Light Chain K88(M) Cyt Tonsil IVD 106 Ki-67 K-2(M) Nuc Tonsil IVD 106 Ki-67 EPR3611(R) Nuc Breast Carcinoma/Tonsil IVD 107 Ki-67 EP5(R) Nuc Tonsil IVD 107 Ki-67 Antigen, Proliferating Cell MIB-1(M) Nuc Lymphoma IVD 107 Ki-67 Antigen, Proliferating Cell Ki88(M) Nuc Tonsil IVD 107 Lambda Light Chain Polyclonal(R) Cyt Tonsil IVD 108 Lambda Light Chain EP172(R) Mem/Cyt Tonsil/B-cell lymphoma IVD 108 Laminin Polyclonal(R) Basement Mem Bronchus IVD 108 Lysozyme Polyclonal(R) Cyt Tonsil,Lymph Node IVD 108 Macrophage LN5(M) Cyt Liver Tissues IVD 109 Mast Cell Tryptase AA1(M) Cyt Skin IVD 109 Melan-A (MART-1) A103(M) Cyt Melanoma IVD 109 Melanoma HMB45(M) Cyt Melanoma IVD 109 Melanoma gp100 gp100/D5(M) Cyt Melanoma tissue IVD 110 Melanoma-Associated Antigen NKI/C3(M) Cyt/Mem Melanoma IVD 110 IVD 110 Mesothelin 5B2(M) Mem Ovary Cyst Adenocarcinoma MiTF MiTF/A13(M) Nuc Melanoma Tissue IVD 110 Mitochondrial Antigen 113-1(M) Cyt Breast Carcinoma IVD 111 MLH1 ES05(M) Nuc Colon Tissue IVD 111 MMP-9 EP1255Y(R) Cyt Bone Marrow IVD 111 MSH2 SP46(R) Nuc Colon IVD 111 MSH6 2D4B5(M) Nuc Colon Carcinoma IVD 112 MUC4 1G8(M) Cyt Colon Carcinoma IVD 112 IVD 112 MUC5AC 45M1(M) Cyt Stomach Mucosa/ Gastrointestinal tissue Mucin 1 (MUC1) EPR1023(R) Cyt Breast Carcinoma IVD 112 Mucin 2 (MUC2) CCP58(M) Cyt Colon Carcinoma IVD 113 Multi-Drug Resistance Marker (P-Glycoprotein) MDR88(M) Mem & Cyt Adrenal Gland IVD 113 Muscle Actins Actin 88 Cocktail(M) Cyt Muscle IVD 113 Myelin Basic Protein MBP88(M) Cyt Cerebellum IVD 113 Myeloid specific Antigen BM-3(M) Cyt Bone Marrow IVD 114 Cyt & Nuc Lymph node,Tonsil,Bone Marrow IVD 114 Myeloid specific Antigen BM-1(M) Please consult the data sheet for pre-treatment and protocol information. Unless specified otherwise, all primary antibodies listed in this table are for FFPE tissue specimens ‡ M: Mouse; R:Rabbit 48 www.biogenex.com Primary Antibodies Antibody Clone (Species) ‡ Localization Positive Control Regulatory Classification Page Myeloperoxidase (MPO) Polyclonal(R) Cyt Spleen IVD 114 Myf4 LO26(M) Nuc Rhabdomyosarcoma IVD 114 Myoglobin MG-1(M) Cyt Muscle IVD 115 Myoglobin Polyclonal(R) Cyt Muscle IVD 115 Myosin Heavy Chains,Smooth Muscle SMMS.1(M) Cyt Breast Carcinoma IVD 115 Myosin,Skeletal Muscle MY-32(M) Cyt Muscle IVD 115 Napsin A IP64(M) Cyt Lung Adenocarcinoma IVD 116 N-Cadherin EPR1792Y(R) Mem Colon Carcinoma Tissue IVD 116 Neurofilament NE-14(M) Cyt Nerve IVD 116 Neuron Specific Enolase (NSE) MIG-N3(M) Cyt Pancreas/Nerve IVD 116 NGF Receptor EP1039Y(R) Mem Neuroblastoma IVD 117 NGF Receptor EP31(R) Cyt Pancreas/Melanoma IVD 117 Nuclear Ribonucleoprotein 58-15(M) Nuc Spleen IVD 117 Oct 2 EPR542(R) Nuc Tonsil tissues IVD 117 Oct 4 EP143(R) Nuc Seminoma in Testis IVD 118 Osteonectin OST1(M) Cyt Osteosarcoma IVD 118 p105 Proliferation-Associated Nuclear Antigen 2B3(M) Nuc Tonsil IVD 118 p16 (INK4a) G175-405(M) Nuc & Cyt Sqamous Cell Carcinoma RUO 118 p21/WAF1 4D10(M) Nuc Melanoma IVD 119 p27 (Kip1) DCS72(M) Nuc Breast Carcinoma IVD 119 p27 (Kip1) Y236(R) Nuc Breast Carcinoma IVD 119 p34cdc2 (Cyclin Dependent Kinase) POH-1(M) Nuc & Cyt Tonsil IVD 119 P504S (AMACR) 13H4(R) Cyt Prostate carcinoma IVD 120 P504S (AMACR) RBT-AMACR(R) Cyt Prostate carcinoma IVD 120 p53 EP9(R) Nuc Breast Ca. IVD 120 p53 Protein BP53-12-1(M) Nuc Breast Carcinoma IVD 120 p53 Protein DO7(M) Nuc Breast Carcinoma IVD 121 p53 Protein 1801(M) Nuc Breast Carcinoma IVD 121 p63 Protein 484(M) Nuc Prostate IVD 121 IVD 121 PAP A40010(M) Cyt Benign Prostate hyperplasia(BPH) and carcinoma prostate Papillomavirus Type 16 (HPV-16) CamVir-1(M) Nuc Cervix IVD 121 PAX-5 ZP007(M) Nuc Lymph Node IVD 122 Paxillin (N-Term) Y113(R) Cyt Breast Carcinoma IVD 122 PDCD4 EPR3432(R) Cyt/Nuc Breast Carcinoma IVD 122 PDGF Receptor-ß Y92(R) Mem GIST IVD 122 PGP9.5 3D9(M) Cyt Brain/Small Intestine IVD 123 Placental Alkaline Phosphatase (PLAP) PL8-F6(M) Cyt Placenta IVD 123 Placental Lactogen (hPL) Polyclonal(R) Cyt Placenta IVD 123 Platelet-Derived Growth Factor (PDGF) PDGF88(M) Cyt Squamous IVD 123 Platelet-Derived Growth Factor (PDGF) Polyclonal(R) Cyt Squamous IVD 124 PMS2 EPR3947(R) Nuc Colon Carcinoma IVD 124 Progesterone Receptor EP2(R) Nuc Breast IVD 124 Progesterone Receptor 1A6(M) Nuc Breast IVD 124 Progesterone Receptor (InSite®PR) PR88(M) Nuc Breast Carcinoma IVD 125 Prolactin ME.121(M) Cyt/Mem Pituitary IVD 125 Please consult the data sheet for pre-treatment and protocol information. Unless specified otherwise, all primary antibodies listed in this table are for FFPE tissue specimens ‡ M: Mouse; R:Rabbit internationalcs@biogenex.com 49 Primary Antibodies Antibody Clone (Species) ‡ Localization Positive Control Regulatory Classification Page Proliferating Cell Nuclear Antigen (PCNA) PC10(M) Nuc Colon Carcinoma IVD 125 Prostate Specific Acid Phosphatase (PSAP) B01-94-21M-NA(M) Cyt Prostate Hyperplasia RUO 125 RUO 126 Prostate Specific Antigen (PSA) ErPr-8(M) Cyt Prostate/Prostate Carcinoma pS2 Estrogen Inducible Protein PS2.1(M) Cyt Breast Carcinoma IVD 126 PSMA EP192(R) Cyt/Mem Prostate Ca. IVD 126 RCC (Renal Cell Carcinoma) RCC-26(M) Cyt/Mem Kidney,Renal Cell Carcinoma IVD 126 S100 beta EP32(R) Cyt Prostate Ca. IVD 127 S100 Protein Polyclonal(R) Nuc and Cyt Melanoma IVD 127 S-100 Protein 15E2E2(M) Nuc & Cyt Melanoma IVD 127 IVD 127 S100P EP186(R) Cyt/Nuc Placenta/Pancreatic Adeno Ca. Sarcomeric Actin ZMSA-5(M) Cyt Muscle IVD 128 Secretin Polyclonal(R) Cyt Stomach IVD 128 Stat 5-Alpha (C-term) E289(R) Cyt & Nuc Breast Carcinoma IVD 128 Substance P Polyclonal(R) Cyt Hypothalamus IVD 128 Survivin EP2880Y(R) Nuc/Cyt TCC Bladder tissue/ Bladder carcinoma IVD 129 Synaptophysin Snp88(M) Cyt Pancreas IVD 129 Tau Tau-2(M) Cyt Cerebellum IVD 129 Tau Tau-5(M) Cyt Brain IVD 129 Terminal Deoxynucleotidyl Transferase (TdT) TdT88(M) Nuc Thymus IVD 130 Terminal Deoxynucleotidyl Transferase (TdT) Polyclonal(R) Nuc Thymus IVD 130 TFPU.1 EPR3159Y(R) Nuc Lymphoma IVD 130 Thyroglobulin 2H11(M) Cyt Follicular adenoma IVD 130 Thyroid Stimulating Hormone (TSH) 5404(M) Cyt Follicular Adenoma IVD 131 Thyroid Stimulating Hormone (TSH) Polyclonal(R) Cyt Follicular Adenoma IVD 131 Thyroid Transcription Factor (TTF-1) 8G7G3/1(M) Nuc Lung IVD 131 Thyroxine D5(M) Cyt Thyroid IVD 131 TIA-1 2G9A10F5(M) Cyt T-cell lymphoma tissue IVD 132 IVD 132 Topo 2 Alpha EP1102Y(R) Nuc/Cyt Thyroid Carcinoma/ Breast Carcinoma) Toxoplasma gondii Polyclonal(R) T.gondii Brain RUO 132 Transferrin HT1/13.6.3(M) Cyt Liver/Muscle IVD 132 Transforming Growth Factor (TGF),Alpha TGF88(M) Cyt Breast Carcinoma IVD 133 Tumor-Associated Glycoprotein (TAG-72) B72.3(M) Cyt Breast Carcinoma IVD 133 Tumor-Associated Glycoprotein (TAG-90 BCA) B6.2(M) Cyt Breast Carcinoma IVD 133 Tyrosinase Ty/G5(M) Cyt Melanoma IVD 133 VEGF Polyclonal(R) Cyt Angiosarcoma IVD 134 Villin ID2C3(M) Cyt and brush border Small Intestine IVD 134 Vimentin V9(M) Cyt Skin/Leiomyoma IVD 134 Vimentin,Non-Hematopoietic LN6(M) Cyt Leiomyoma IVD 134 VIP Polyclonal(R) Cyt Small Intestine IVD 135 Wilm’s Tumor CAN-R9(IHC)-56-2(R) Nuc Wilm’s Tumor IVD 135 ZAP-70 ZAP70-C3(M) Cyt/Mem Tonsil and Reactive lymph node IVD 135 ZAP-70 YE291(R) Mem/Cyt Tonsil IVD 135 Please consult the data sheet for pre-treatment and protocol information. Unless specified otherwise, all primary antibodies listed in this table are for FFPE tissue specimens ‡ M: Mouse; R:Rabbit 50 www.biogenex.com Primary Antibodies Aberrant Endothelial Cell Actin, Muscle-Specific P P Tonsil stained with Anti-Aberrant Endothelial cell using AEC chromogen Clone: 4A11 Clone: HHF35 Isotype: IgM Isotype: IgG1 Source: Mouse Source: Mouse Immunogen: Human rheumatoid cells Immunogen: Homogenized human myocardium Specificity: Vascular endothelial cell Antigen Specificity: Muscle-specific Actin Localization: Cytoplasm Localization: Cytoplasm Pre-treatment: None Pre-treatment: EZ-AR1 Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® AX382-YCD Concentrated: MU382-UC Heart muscle stained with AntiMuscle Specific Actin using DAB chromogen AM382-5M In an in vivo model of allergic contact dermatitis, 4A11 antigen was differentially upregulated from other endothelial markers such as E-selectin, vascular cell adhesion molecule-1, and intercellular adhesion molecule-1. Monoclonal antibody 4A11 reacts with the H-5-2 and Lewis Y-6 blood group glycolipids. This antibody reacts with vascular endothelial cells in lymphoid tissues and endothelial cells in diseased tissue such as rheumatoid and osteoarthritic synovium, psoriatic skin, adrenal tumors and cutaneous Kaposi’s sarcomas. It does not react with several myeloid or lymphoid cell lines, peripheral blood cells and platelets. It does not detect endothelium of mediumsized vessels and that of normal tissues such as liver and spleen. ACTH P Pituitary tissue stained with AntiACTH using DAB chromogen Clone: AH26 Isotype: IgG Source: Mouse Immunogen: A synthetic peptide corresponding to amino acids1-24 from the N-terminal of human ACTH Specificity: ACTH Localization: Cytoplasm Pre-treatment: EZ-AR™ 2/ EZ-AR1 Manual/i6000: HK522-XAK Xmatrx: HX031-YCDE Ready-to-use (Manual): AM487-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM487-10M AX487-YCD Recommended Positive Control: FG-487N AM090-10M AX090-YCD Concentrated: MU090-UC Recommended Positive Control: FG-090M Recommended Barrier Control: FB-090M Actin, a major component of the cytoskeleton, is a globular protein about 5 nm in diameter and is composed of one polypeptide chain with a mass of approximately 47kD. This antibody recognizes alpha actin of skeletal, cardiac and smooth muscle cells and gamma actin from smooth muscle cells. It is non-reactive with other mesenchymal cells and all epithelial cells except for myoepithelium. It can be used to stain leiomyomas, leiomyosarcomas, rhabdomyomas and rhabdomyosarcomas. This antibody labels cytoplasm in skeletal, cardiac and smooth muscle cells. Actin, Smooth Muscle P Stomach tissue stained with AntiSmooth Muscle Actin using DAB chromogen MU487-UC Adrenocorticotropic hormone (ACTH or Corticotropin) is a polypeptide tropic hormone produced and secreted by the anterior pituitary gland. It is an important component of the hypothalamic-pituitary-adrenal axis and is often produced in response to biological stress (along with corticotrophin-releasing hormone from the hypothalamus). Its principal effects are increased production of androgens and as its name suggests, cortisol from the adrenal cortex. It labels corticotrophs in the adenohypophysis and is useful in the classification of pituitary adenomas. internationalcs@biogenex.com AM090-5M Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Clone: 1A4 Isotype: IgG2a Source: Mouse Immunogen: Synthetic NH2 terminal decapeptide of alpha smooth muscle actin coupled to keyhole limpet hemocyanin (KLH) Specificity: Alpha Smooth Muscle Actin Localization: Cytoplasm Pre-treatment: None Ready-to-use (Manual): AM128-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM128-10M AX128-YCD Recommended Positive Control: FG-128M Recommended Barrier Control: FB-128M MU128-UC Actin is one of the two major cytoskeletal proteins. The antibody can be used to identify smooth muscle tumors. It stains leiomyomas and leiomyosarcomas but does not stain carcinomas, melanomas, lymphomas or non-smooth muscle sarcomas. It stains the muscularis and muscularis mucosa of the gastrointestinal tract, the uterine myometrium, medial layer of blood vessels, the mesenchymal components of the prostate, and myoepithelial cells of salivary glands and other organs. The antibody does not stain striated muscle such as skeletal and cardiac muscle, endothelium, connective tissue, epithelium or nerve. This antibody stains positive in cytoplasm of smooth muscle cells. P - Tissue Type FFPE F - Tissue Type Frozen 51 Primary Antibodies Adenovirus Alpha-1-Antitrypsin F A62020069P Clone: Polyclonal Isotype: IgG1 Kappa Isotype: N/A Source: Mouse Source: Rabbit Immunogen: Adeno 3 strain Immunogen: Human plasma Specificity: Adenovirus antigen/ immunogen in frozen tissue sections or infected cell culture. Specificity: Alpha-1-Antitrypsin Localization: Adenovirus cell culture stained using AEC chromogen P Clone: Nucleus & Cytoplasm Pre-treatment: EZ-AR 1/EZ-AR 2 Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Concentrated: Recommended Positive Control: AM059-5ME AX059-YCDE MU059-UCE FG-059M Adenoviruses are simple DNA-containing viruses that multiply in the cell nucleus. They induce latent infections in tonsils, adenoids and other lymphoid tissue of man, causing either inapparent or limited illnesses that are followed by complete recovery and persistent typespecific immunity. Most individuals are infected with one or more adenovirus before the age of 15. Approximately 50-80% of tonsils and adenoids removed surgically show adenovirus infection. This antibody is designed for the specific immunohistochemical localization of adenovirus. Monoclonal antibodies directed against viruses allow for the rapid identification of viral infections in tissues. The advantages of identifying viruses immunohistologically are twofold. First, this method decreases the time required for microbial identification by viral culture, and second, this method may demonstrate virus in tissues in which characteristic cytopathic effects have not yet developed and are therefore not detectable by morphological studies alone. Localization: Cytoplasm Pre-treatment: EZ-AR 1/EZ-AR 2 Xmatrx: HX031-YCDE/HX032-YCDE Liver tissue stained with Anti-alpha1-Antitrypsin using AEC chromogen Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® AR015-5R Concentrated: AW015-YCD PU015-UP Recommended Positive Control: FG-015P Alpha-1-Antitrypsin (AAT) is a 54 kD glycoprotein. Most of the antiproteolytic enzyme activity of serum resides in this fraction. It is also found in lymph, mucus, saliva, synovial fluid, gastrointestinal tract secretions, semen, amniotic fluid and colostrum. It is a useful marker for benign and malignant hepatic neoplasms, endodermal sinus tumors, and for histiocytic differentiation in benign and malignant fibrous histiocytomas. This antibody has been absorbed with fractionated human plasma to remove contaminating antibodies. When tested by crossed immunoelectrophoresis against human plasma, a single precipitin line was observed. Alpha-Actinin P Alpha-1-Antichymotrypsin Clone: JLN20 Isotype: IgM Source: Mouse Immunogen: Alpha-actinin isolated from chicken gizzard Alpha-Actinin P Liver stained with Anti-ACT using AEC chromogen Ready-to-use (Manual): Clone: α1A88 Specificity: Isotype: IgG1, Kappa Localization: Cytoplasm Source: Mouse Pre-treatment: EZ-AR 1/EZ-AR 2 Immunogen: Biochemically purified alpha-1-antichymotrypsin protein was used to sensitize Balb/c (H-2d) mice Xmatrx: HX031-YCDE/HX032-YCDE Specificity: Alpha-1-Antichymotrypsin protein Localization: Cytoplasm Pre-treatment: EZ-AR 1 Xmatrx HX031-YCDE AM388-5M Ready-to-use (Automated): Xmatrx® AX388-YCD Concentrated: MU388-UC Muscle stained with Anti-alpha actinin using AEC chromogen Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® AM097-5M AX097-YCD Concentrated: MU097-UC Recommended Positive Control: FG-097M Actinins are actin-binding proteins of 100 kD. Alpha-Actinin is an F-actin cross-linking protein thought to anchor actin to a variety of intracellular structures. Alpha-Actinin is found in stress fibers and adhesion plaques in non-muscle cells and in Z-discs and their homologues in muscle cells. Alpha-1-Antichymotrypsin (ACT) is a serine protease inhibitor. It forms a complex with serine protease, a prostate-specific antigen in human serum. ACT can be found in most cells of myeloid lineage and is, therefore, useful in the identification of neoplastic myeloid cells within extramedullary tissues such as acute myeloid leukemia. This enzyme is also localized in the spindle cells and round cells of true histiocytic lymphomas as well as in most thyroid papillary carcinomas. ACT is expressed in various normal and neoplastic cells. The mouse monoclonal antibody stains ACT protein in the cytoplasm of many different cells. 52 P - Tissue Type FFPE F - Tissue Type Frozen www.biogenex.com Primary Antibodies Alpha-Fetoprotein (AFP) Androgen Receptor P P Clone: C3 Clone: F39.4.1 Isotype: IgG 2a Isotype: IgG1 Kappa Source: Mouse Source: Mouse Immunogen: Affinity-purified human Alpha-Fetoprotein Immunogen: Synthetic peptide sequence comprising amino acids 301-320 of human androgen receptor (SP61). Specificity: Androgen Receptor antigen Specificity: Alpha-Fetoprotein Localization: Cytoplasm Pre-treatment: None Hepatocellular carcinoma stained with Anti-AFP using AEC chromogen Prostate Hyperplasia showing nuclear Androgen Receptor positivity using DAB chromogen AM008-5M Ready-to-use (Manual): Localization: Nuclear Pre-treatment: AR Citra/EZ AR-2 Manual/i6000: HK087-5KE Xmatrx: Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM008-10M AX008-YCD Recommended Positive Control: MU008A-UC FG-008M Recommended Barrier Control: FB-008M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Recommended Positive Control: Alpha-Fetoprotein (AFP) is a 64 kD tumor-associated embryonal antigen produced by fetal liver, hepatoma, yolk sac, and several germ cell tumors of testicular and ovarian origin. Of the germ cell tumors, only embryonal carcinoma and endodermal sinus tumors stain positive for AFP and not teratomas. The positive results are useful in distinguishing embryonal carcinoma from seminoma. AFP is present in the mononuclear embryonal carcinoma cell and in the intracellular or extracellular hyaline droplets. This antibody stains positive for alpha fetoprotein in the cytoplasm of positive cells. Recommended Barrier Control: AM256-10ME AX256-YCDE MU256-UCE FG-256M FB-256M This monoclonal antibody is specifically designed to recognize a unique immunogenic N-terminal transactivation domain of the androgen receptor that has a low degree of homology with other steroid receptors. This antibody binds to synthetic peptide SP61 of human androgen receptor. This antibody does not cross-react with human estrogen, progesterone or glucocorticoid receptor. Apolipoprotein E Alpha-Tubulin HX032-YCDE AM256-2ME Ready-to-use (Manual 3mL): P Clone: EP1373Y Clone: DM-1A Isotype: IgG Isotype: IgG1 Source: Rabbit Source: Mouse Immunogen: Immunogen: Alpha-Tubulin isolated from chick brain microtubules A synthetic peptide corresponding to residues near the N-term of human apolipoprotein E Specificity: Apolipoprotein E Localization: Membrane and Cytoplasm Pre-treatment: AR Citra Plus /EZ-AR 1/ EZ-AR 2 Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE/HX032-YCDE P Specificity: Alpha-Tubulin Localization: Cytoplasm Pre-treatment: None Hepatocellular carcinoma stained with Anti-Apolipoprotein E using DAB as chromogen Lung tissue stained with Anti-AlphaTubulin using AEC chromogen Concentrated: MU121-UC Recommended Positive Control: FG-121M Microtubules, along with microfilaments and intermediate filaments, form the major part of the extensive cytoplasmic network known as the cytoskeleton. The thickest of these filaments are the 20-25 nm microtubules composed of tubulin and several additional microtubuleassociated proteins (MAP). Tubulin is a heterodimer composed of α-tubulin and β-tubulin. Each subunit is a 55 kD acidic protein. Tubulin assembles into the microtubule system during interphase, then reassembles into the mitotic spindle during cell division. This antibody reacts specifically with the alpha subunit of tubulin in cultured chicken fibroblasts, human, bovine, murine, and amphibian cells, and also in yeast and fungi. internationalcs@biogenex.com Ready-to-use (Manual): AN567-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN567-10M AX567-YCD Recommended Positive Control: FG-567N NU567-UC Apolipoprotein E (ApoE) is essential for the normal catabolism of triglyceride rich lipoprotein constituents. Defects in the ApoE gene will result in familial dysbetalipoproteinemia or type III hyperlipoproteinemia and will increase cholesterol and triglycerides levels in the blood. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes. There are three isoforms of the ApoE lipoprotein, known as ApoE2, E3 and E4. ApoE4 has been associated with an increased probability of developing Alzheimer disease. P - Tissue Type FFPE F - Tissue Type Frozen 53 Primary Antibodies B Cell Bax Protein P Tonsil tissue stained with Anti-B cell using Fast Red Chromogen P Clone: MB2 Clone: Polyclonal Isotype: IgG1 Source: Rabbit Source: Mouse Immunogen: Immunogen: Hodgkin’s lymphoma cell line DEV A synthetic peptide encompassing a unique epitope at the amino terminus of human Bax protein coupled to Keyhole Limpet Hemocyanin (KLH) Specificity: Bax protein Specificity: MB2 Localization: Cytoplasm Pre-treatment: EZ-AR 1 Manual/i6000: None Xmatrx: HX031-YCDE Ready-to-use (Manual): AM158-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM158-10M AX158-YCD Recommended Positive Control: FG-158M MU158-UC MB2 reacts with a cytoplasmic antigen present in all B cells with the exception of plasma cells. It also reacts with endothelial cells and various types of epithelial cells. MB2 shows no reaction with T lymphocytes or thymocytes. A faint staining may occur when using frozen sections containing T cells. MB2 is not suitable for immunolabeling of living or unfixed cells. Breast carcinoma stained with AntiBax Protein using DAB chromogen Hodgkin stained with Anti-BLA.36 using DAB chromogen A27-42 IgG3 EZ-AR 1/EZ-AR 2 Xmatrx: HX031-YCDE/HX032-YCDE AR347-5R AW347-YCD PU347-UP Bax protein is identified as a promoter of apoptosis. The override of apoptotic control is suspected to cause or contribute to some forms of carcinogenesis. This antibody will detect the α, ß , and d isoforms of Bax protein. Bcl-2 P P Isotype: Membrane Pre-treatment: Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Concentrated: B Lymphocyte Antigen 36 (BLA.36) Clone: Localization: Clone: E17 Isotype: IgG Source: Rabbit Immunogen: A synthetic peptide corresponding to residues between BH3 and BH4 of human Bcl-2 Bcl-2 protein Source: Mouse Specificity: Immunogen: Hodgkin's cell line HDLM-3 Localization: Cytoplasm Pre-treatment: EZ-AR™ 1 Manual/i6000: HK521-XAK Specificity: BLA.36 antigen Localization: Membrane Pre-treatment: EZ-AR 1/EZ-AR 2 Manual/i6000: None Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): AM231-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM231-10M AX231-YCD Recommended Positive Control: FG-231M Recommended Barrier Control: FB-231M MU231-UC Follicular cell lymphoma stained with Rabbit Anti-Bcl-2 using DAB as a chromogen Xmatrx: Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Recommended Positive Control: HX031-YCDE AN541-5M AN541-10M AX541-YCD NU541-UC FG-541N Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis. It has been documented that Bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore activated in B cell malignancies. BLA.36 is a developmentally regulated 36 kD antigen expressed on the plasma membrane of B lymphocytes, Reed-Sternberg, and mononuclear Hodgkin’s cells. The anti-BLA.36 antibody recognizes all four subtypes of Hodgkin's disease. It also gives strong staining of B cell lymphomas including follicular center cell lymphomas (large and small cell types), mantle zone lymphomas, and immunoblastic lymphomas. No reactivity of anti-BLA.36 is found in normal epithelial cells, including adrenal gland, breast, colon, lung, salivary gland, skin, stomach and their malignant counterparts. Anti-BLA.36 can be used to distinguish Reed-Sternberg cells and some B-cell lymphomas from other malignant cells. 54 P - Tissue Type FFPE F - Tissue Type Frozen www.biogenex.com Primary Antibodies Bcl-2 Bcl-6 P Tonsil stained with Anti-Bcl 2 using DAB chromogen EP36 Clone: LN22 Isotype: IgG1 Isotype : IgG Source: Rabbit Source: Mouse Immunogen: BCl-2 Immunogen: Bcl-6 Specificity: BCl-2 Specificity: Bcl-6 Localization: Cytoplasm Localization: Nuclear Pre-treatment: AR-10 Pre-treatment: AR-10 Manual/i6000: HK058-5K Manual/i6000: HK058-5K Xmatrx: None Xmatrx: None Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Recommended Positive Control: Recommended Barrier Control: Tonsil stained with Anti-Bcl-6 using DAB chromogen AN723-5M AN723-10M AY723-YCD FG-723N FB-723N Bcl-2 (B-cell lymphoma 2), encoded in humans by the Bcl-2 gene, is the founding member of the Bcl-2 family of regulator proteins that regulate cell death, by either inducing (pro-apoptotic) it or inhibiting it (anti-apoptotic). Bcl-2 is specifically considered as an important anti-apoptotic protein and is thus classified as an oncogene. Over expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis. It has been documented that Bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore activated in B cell malignancies. Bcl-2 is useful in differentiation of follicular lymphoma from reactive follicular proliferation (Bcl-2 negative). In addition, Bcl2 has been shown to be correlated with disease prognosis in breast cancer, prostate and ovarian cancer. BcI-2 Oncoprotein P Clone: IgG1 kappa Source: Mouse Synthetic peptide comprising residues 41-54 of Bcl-2 oncoprotein-3 Specificity: Bcl-2 protein Localization: Cytoplasm Pre-treatment: AR Citra/EZ-AR 1 Manual/i6000: HK087-5KE Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Recommended Positive Control: Recommended Barrier Control: AM708-5M AM708-10M AY708-YCD Recommended Positive Control: FG-708M Recommended Barrier Control: FB-708M Anti-BCl-6 is a transcriptional regulator gene which codes for a 706 amino acid nuclear zinc finger protein. This antibody reacts with Bcl-6 gene product in follicular lymphomas, diffuse large B-cell lymphomas. Burkitt’s lymphomas and in nodular lymphocyte predominant Hodgkin’s disease. The antibody gives a strong nuclear labeling of BCL6 protein in follicular lymphomas, diffuse large B-cell lymphomas, Burkitt’s lymphomas and nodular, lymphocyte=predominance Hodgkin’s disease. BCl-6 is not expressed in B-CLL, hairy cell leukemia, mantle and marginal-Zone derived lymphomas. Bcl-x P Breast carcinoma showing cytoplasmic expression of Bcl-x stained using DAB chromogen AM287-5M AM287-10M AX287-YCD FG-287M FB-287M The Bcl-2 is an integral inner mitochondrial membrane protein and is frequently overexpressed in many lymphoid malignancies. Immunohistologic studies have demonstrated that staining for Bcl-2 protein can be used to distinguish neoplastic germinal centers from reactive ones. internationalcs@biogenex.com Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® bcl-2/100 Isotype: Immunogen: Tonsil tissue stained with Anti-Bcl-2 using DAB chromogen P Clone: Clone: E18 Isotype: IgG Source: Rabbit Immunogen: Human Bcl-x Specificity: Bcl-x Localization: Membrane Pre-treatment: EZ-ARTM 1 Manual/i6000: HK521-XAK Xmatrx: HX031-YCDE Ready-to-use (Manual): AN462-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN462-10M AX462-YCD NU462-UC Recommended Positive Control: FG-462N Bcl-x, a member of the Bcl-2 protein family, inhibits cell death, or apoptosis. Bcl-x is expressed as two isomeric forms, Bcl-xL and Bcl-xS, and is typically present in the cytosol in association with the mitochondrial membrane. Bcl-xL forms heterodimers with various proteins, including Bax, Bak and Bcl-2. It has been found that heterodimerization with Bax does not seem to be required for antiapoptotic activity. Since Bcl-xL can form an ion channel in synthetic lipid membranes, there is a strong possibility that this property plays a role in heterodimerization-independent cell survival. The Bcl-xS isoform promotes apoptosis. P - Tissue Type FFPE F - Tissue Type Frozen 55 Primary Antibodies Beta-Catenin Beta-Tubulin II P Colon stained with Antiß-Catenin using DAB chromogen P Clone: E247 Clone: JDR3B8 Isotype: IgG Isotype: IgG2b Source: Rabbit Source: Mouse Immunogen: A synthetic peptide corresponding to residues near N- terminus of human β - catenin. Immunogen: Cys-Glu-Gly-Glu-GluAsp-Glu-Ala-OH synthetic peptide conjugated with BSA. Specificity: ß-Tubulin II Specificity: ß-Catenin Localization: Membrane/Cytoplasm/ Nucleus Pre-treatment: EZ-AR™ 1/EZ-AR 2 Manual/i6000: HK521-XAK Xmatrx: HX031-YCDE Ready-to-use (Manual): AN510-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN510-10M AX510-YCD Recommended Positive Control: FG-510N NU510-UC ß-catenin is a key regulatory protein involved in cell adhesion and signal transduction through the Wnt pathway, and plays important roles in development, cellular proliferation, and differentiation. ß-catenin is phosphorylated at four serine and threonine residues at the N-terminus. Ser 45 is phosphorylated by CK1, and is thought to be a priming site for the phosphorylation by glycogen synthase kinase-3 (GSK-3) of the remaining three residues, Thr 41, Ser 37, and Ser 33. Wnt signaling antagonizes the action of GSK-3, which in subsequent phosphorylation steps, leads to its degradation. Mutations in the catenin gene, CTNNB1, have been implicated in its accumulation and various forms of carcinomas. Colon stained with Anti-Beta Tubulin II using AEC chromogen Clone: DM-1B Isotype: IgG1 kappa Source: Mouse Immunogen: Beta-tubulin isolated from chick brain microtubules Specificity: Beta-Tubulin Localization: Cytoplasm Pre-treatment: None Lung tissue stained with Anti-Beta Tubulin using AEC chromogen Concentrated: MU122-UC Recommended Positive Control: FG-122M Microtubules, along with microfilaments and intermediate filaments, form the major part of the extensive cytoplasmic network known as the cytoskeleton. The thickest of these filaments are the 20-25 nm microtubules composed of tubulin and several additional microtubuleassociated proteins (MAP). Tubulin is a heterodimer composed of α-tubulin and ß-tubulin. Each subunit is a 55 kD acidic protein. Tubulin assembles into the microtubule system during interphase, then reassembles into the mitotic spindle during cell division. Immunoblot analysis shows that this antibody binds to the beta subunit of tubulin from cultured fibroblasts and chick brain tubulin. This antibody labels the cytoplasmic network of microtubules and mitotic spindles of cultured cells. 56 P - Tissue Type FFPE F - Tissue Type Frozen Cytoplasm Pre-treatment: None Concentrated: MU176-UC Recommended Positive Control: FG-176M Microtubules play critical roles in a variety of cellular processes, such as mitosis, intracellular transport, ciliary and flagellar motility, and maintenance of cell shape. The structural subunit of microtubules, the 100 kD protein tubulin, is a heterodimer of two 50 kD subunits designated alpha and beta. Both alpha and beta occur as numerous isotypes which differ from each other in their amino acid sequences and tissue distribution. The majority of the differences among the isotypes cluster in the C-terminal, a region where the microtubuleassociated proteins (MAPs) bind to tubulin. This antibody stains ß-tubulin in cytoplasm of neuroepithelial cells and other positive cells. Beta-Tubulin III P Beta-Tubulin P Localization: Heart muscle stained with Anti-Beta Tubulin III using DAB chromogen Clone: SDL3D10 Isotype: IgG2b Source: Mouse Immunogen: Cys-Glu-Ser-Glu-SerGlu-Gln-Gly-Pro-LysOH synthetic peptide conjugated with BSA. Specificity: ß-Tubulin III Localization: Cytoplasm Pre-treatment: None Concentrated: MU177-UC Recommended Positive Control: FG-177M Microtubules play critical roles in a variety of cellular processes, such as mitosis, intracellular transport, ciliary and flagellar motility, and maintenance of cell shape. The structural subunit of microtubules, the 100 kD protein tubulin, is a heterodimer of two 50 kD subunits designated alpha and beta. Both alpha and beta occur as numerous isotypes which differ from each other in their amino acid sequences and tissue distribution. The majority of the differences among the isotypes cluster in the C-terminal, a region where the microtubuleassociated proteins (MAPs) bind to tubulin. This antibody stains beta tubulin in cytoplasm of positive cells. www.biogenex.com Primary Antibodies Beta-Tubulin IV Blood Group Antigen Lewis A P Lung stained with Anti-Beta Tubulin IV using AEC chromogen P Clone: ONS1A6 Clone: 7LE Isotype: IgG1 Isotype: IgG1 Source: Mouse Source: Mouse Immunogen: Cys-Glu-Ala-Glu-GluGlu-Val-Ala-OH synthetic peptide conjugated with BSA 1 Immunogen: Mucin isolated from ovarian cyst fluid Specificity: Blood Group Antigen Lewis A Specificity: ß-Tubulin IV Localization: Localization: Cytoplasm Cytoplasm and Membrane Pre-treatment: None Pre-treatment: None Concentrated: MU178-UC Recommended Positive Control: FG-178M Stomach tissue stained with AntiBlood group antigen Lewis A using AEC chromogen Microtubules play critical roles in a variety of cellular processes, such as mitosis, intracellular transport, ciliary and flagellar motility, and maintenance of cell shape. In the structural subunit of microtubules, the 100 kD protein tubulin, is a heterodimer of two 50 kD subunits designated alpha and beta. Both alpha and beta occur as numerous isotypes which differ from each other in their amino acid sequences and tissue distribution. The majority of the differences among the isotypes cluster in the C-terminal, a region where the microtubuleassociated proteins (MAPs) bind to tubulin. This antibody stains ß-tubulin in cytoplasm of positive cells. Concentrated: MU303-UC Recommended Positive Control: FG-303M Lewis blood group antigens are carbohydrate moieties structurally integrated in mucous secretions. Lewis antigen system alterations have been described in gastric carcinoma and associated lesions. Enhanced expression of Lewis A antigen has been found in malignant transformation of gastric tissues. A panel of antibodies to Lewis antigens, including Lewis A, Lewis B and sialylated Lewis A, is useful in the immunopathological analysis of gastric cancers. Clone 7LE detects Lewis A antigen in cultured cells and tissue sections by immunohistochemistry and reacts with the immunogen in ELISA assays. This antibody stains blood group antigen Lewis A. bFGF (Basic Fibroblast Growth Factor) P Adenocarcinoma stained with AntibFGF using AEC chromogen Blood Group Antigen Lewis B Clone: bFGF88 Isotype: IgG 2b Source: Mouse Immunogen: A unique synthetic peptide of bFGF coupled to keyhole limpet hemocyanin Specificity: bFGF Localization: Cytoplasm Pre-treatment: EZ-AR 1 Xmatrx: HX031-YCDE Ready-to-use (Manual): AM359-5M Ready-to-use (Automated): Xmatrx® AX359-YCD P 2-25LE Isotype: IgG1 Source: Mouse Immunogen: Mucin isolated from ovarian cyst fluid Specificity: Blood Group Antigen Lewis B Localization: Cytoplasm and Membrane Pre-treatment: None Intestine stained with Anti-Blood group Lewis B antibody using AEC chromogen bFGF is a pro-angiogenic cytokine which is present in diverse tissues. It is known to function as an autocrine mediator of mitogenesis of endothelial cells in vivo, resulting in angiogenesis. It also increases fibroblast production of plasminogen activator and collagenase. bFGF is a heparin binding cytokine that is found inside cells and in extracellular stores bound to heparin or heparin sulfate proteoglycans. bFGF may be released to mediate tissue repair since expression is known to be high in mast cells responding to injury. The monoclonal antibody to bFGF can be used for the study of myometrial smooth muscle cells, uterine leiomyomas, cardiac myocytes, arterial endothelium, gastric carcinoma, and invasive or metastatic melanoma. This antibody stains bFGF in cytoplasm of many diverse cell types. internationalcs@biogenex.com Clone: Concentrated: MU304-UC Recommended Positive Control: FG-304M Lewis blood group antigens are carbohydrate moieties structurally integrated in mucous secretions. Lewis antigen system alterations have been described in gastric carcinoma and associated lesions. Anomalous expression of Lewis B antigen has been found in some nonsecretory gastric carcinomas and colorectal cancers. This antibody will stain Lewis B antigen in formalin-fixed, paraffin-embedded tissues. A panel of antibodies to Lewis antigens, including Lewis A, Lewis B and sialylated Lewis A, is useful in the immunopathological analysis of gastric cancers. Monoclonal antibody 2-25LE detects Lewis B antigen in cultured cells and tissue sections using immunohistochemistry and will react with the immunogen in ELISA assays. This antibody stains blood group antigen Lewis B. P - Tissue Type FFPE F - Tissue Type Frozen 57 Primary Antibodies BOB-1 Breast Cancer Antigen BCA-225 P Tonsil stained with anti-BOB-1 using DAB chromogen P Clone: SP92 Clone: CU18 Isotype: IgG Isotype: IgG1 Source: Rabbit Source: Mouse Immunogen: Synthetic peptide corresponding to C-terminus of human BOB-1 protein Immunogen: RNA virus-like particles from T47D breast carcinoma cell line (VR). Specificity: Human BOB-1 protein Specificity: Localization: Nucleus Breast carcinoma Associated Antigen (BCA-225) Pre-treatment: EZ-AR 2 Manual/i6000 HK522-XAK Xmatrx None Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN731-5ME Recommended Positive Control: Recommended Barrier Control: AN731-10ME AY731-YCDE NU731-UCE FG-731N FB-731N B cell Oct binding protein 1 (BOB-1), is a transcription factor that is required for germinal center formation and immunoglobulin production. Various lymphomas are positive for this marker including the following: B-Chronic lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, plasmacytoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, T-cell rich B-cell lymphoma, nodular lymphocyte predominant Hodgkin lymphoma, whereas classic Hodgkin lymphoma is usually negative. BRCA1 Protein Localization: Cytoplasm Pre-treatment: EZ-AR 1 Manual/i6000 None Xmatrx: HX031-YCDE Ready-to-use (Manual): AM135-5M Ready-to-use (Automated): i6000TM Xmatrx® Recommended Positive Control: AM135-10M AX135-YCD FG-135M This antibody recognizes a 225-250 kD glycoprotein found in most human breast carcinomas and a few other tissues. CU18 does not stain lactating mammary gland. CA19-9 P P Anti-BRCA1 positivity in recurrent tumor using DAB chromogen Clone: C241:5:1:4 Isotype: IgG1 Source: Mouse Immunogen: Human colorectal adeno carcinoma cell line COLO205 Specificity: CA19-9 protein Clone: Polyclonal Localization: Cytoplasm Source: Rabbit Pre-treatment: AR Citra Plus/ EZ-AR 2 Immunogen: A synthetic peptide encompassing a unique epitope within the carboxyl terminal domain of human BRCA1 coupled to Keyhole Limpet Hemocyanin. Manual/i6000 HK081-5KE Xmatrx: HX032-YCDE Specificity: BRCA1 Localization: Nucleus and Cytoplasm Pre-treatment: AR Citra/ /EZ-AR 1/ EZ-AR 2 Manual: HK087-5KE Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® AW345-YCD Concentrated: PU345-UP AR345-5R The BRCA1 gene was discovered as a region on chromosome 17q21 that has a high frequency of mutation in families predisposed to breast cancer. Specific mutations and variability in expression have been identified and characterized, including the founder mutation 185delAG in Askenazi Jewish families. BRCA1 functions as a tumor suppressor by mechanisms not yet understood. It has recently been suggested that BRCA1 might induce apoptosis similar to the gatekeeper function of the p53 tumor suppressor. This antibody reacts with an epitope mapping near the carboxyl terminus of the normal (non-mutant) BRCA1 gene product. 58 Breast carcinoma stained with AntiBCA-225 using AEC chromogen P - Tissue Type FFPE F - Tissue Type Frozen Colon stained with Anti-CA19-9 using DAB chromogen Ready-to-use (Manual): AM424-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM424-10M AX424-YCD MU424-UC Recommended Positive Control: FG-424M Recommended Barrier Control: FB-424M Carcinoma Antigen 19-9 (CA19-9) is a carbohydrate antigen that reacts specifically with Sialyl Lewis-containing glycolipids and has been isolated and characterized as the oligosaccharide sialylazed lacto-N-fucopentose II antigen. This monoclonal antibody is directed against the CA19-9 antigen, which is expressed in pancreatic carcinomas, hepatobillary carcinomas, the tumor cells of colorectal and gastric cancers.. It can also be found in chronic pancreatitis and in healthy colonic mucosa of patients with colorectal cancer. www.biogenex.com Primary Antibodies CA 125 (Ovarian Tumor Marker) Caldesmon, High MW, Smooth Muscle P P Clone: Ov185:1 Isotype: IgG1 Source: Mouse Immunogen: Specificity: Ovarian carcinoma stained with Anti-Ovarian Tumor Marker (CA125) using DAB chromogen A partially purified mucin fraction from a pool of cancer tissues from patients with epithelial ovarian cancer. Repetitive protein determinant expressed in the protein core of CA125 human ovarian cancer antigen. IgG1 Source: Mouse Immunogen: Crude human uterus caldesmon Specificity: Caldesmon, high molecular weight Localization: Cytoplasm Pre-treatment: AR-10/EZ-AR 1 Manual: HK058-5K Xmatrx: HX031-YCDE Membrane/Cytoplasm Ready-to-use (Manual): AM332-5M Pre-treatment: AR-10/EZ-AR 1/EZ-AR 2 Manual/i6000: HK058-5K AX332-YCD Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Automated): Xmatrx® Concentrated: AM429-5M Ready-to-use (Automated): i6000TM Xmatrx® AM429-10M AX429-YCD Concentrated: MU429-UC Recommended Positive Control: FG-429M Recommended Barrier Control: FB-429M Monoclonal antibody Ov185:1 reacts with repetitive protein determinant expressed in the protein core of the CA125 human ovarian cancer antigen. This marker is usually associated with ovarian epithelial malignancies. Immunohistochemistry with CA125 antibody in conjunction with other markers was found to be useful in tracing the origin of adeno carcinoma of unknown origin. This antibody stains membrane in ovarian cancer cells. Clone: E89 Isotype: IgG Source: Rabbit Immunogen: A synthetic phosphorpeptide corresponding to residues surrounding ser789 in the C-terminus of human caldesmon Specificity: Caldesmon Localization: Cytoplasm Pre-treatment: EZ-AR™ 1 Manual/i6000: HK521-XAK Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN507-5M Recommended Positive Control: FG-507N AN507-10M AX507-YCD NU507-UC Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin. Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosinbinding domain at the N-terminus. Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calciumcalmodulin and is promoted by tropomyosin. internationalcs@biogenex.com MU332-UC Caldesmon is considered to be the marker for smooth muscle cell phenotype. Monoclonal antibody to caldesmon, high molecular weight (120-150kD), in combination with monoclonal antibodies to calponin and smooth muscle myosin heavy chains could be used to distinguish benign and in-situ lesions from invasive carcinomas. Anti-caldesmon antibody may be used to characterize the differentiation process of mammary myoepithelial cells in the developing mammary gland, investigate the nature of myoepithelial cells and study the development of human smooth muscle cells. This antibody stains caldesmon in cytoplasm of vascular, visceral smooth muscle and myoepithelial cells in normal and benign human mammary gland. Calponin P P Uterus (Endomyo) stained with AntiCaldesmon using AEC chromogen h-CD Isotype: Localization: Ready-to-use (Manual): Caldesmon Smooth muscle stained with AntiCaldesmon using DAB Clone: Myoepithelial cells in normal breast highlighted by Calponin stained using DAB chromogen Clone: CALP Isotype: IgG1 Source: Mouse Immunogen: Crude human uterus extract Specificity: Phosphorylated tyrosine Localization: Cytoplasm Pre-treatment: AR-10/EZ-AR 1 Manual: HK058-5K Xmatrx: HX031-YCDE Ready-to-use (Manual): AM333-5M Ready-to-use (Automated): Xmatrx® Concentrated: AX333-YCD Recommended Positive Control: FG-333M MU333-UC Calponin is a 33 kD thin filament-associated protein that plays a role in regulation of smooth muscle contractility by anchoring myosin to actin. Monoclonal antibody to Calponin in combination with clones SMMS1(anti-myosin heavy chain antibody) and h-CD (anti-Caldesmon antibody) could be used to distinguish benign and in-situ lesions from invasive carcinomas. This antibody stains Calponin in cytoplasm of vascular and visceral smooth muscle cells, myoepithelial cells in normal and benign human mammary gland, and certain stromal myofibroblasts. P - Tissue Type FFPE F - Tissue Type Frozen 59 Primary Antibodies Calponin-1(C-term) Calretinin P Salivary gland tumor (Pleomorphic adenoma) tissue stained with Anti-Calponin-1 (C-term) using AEC chromogen P Clone: EP798Y Clone: Polyclonal Isotype: IgG Isotype: N/A Source: Rabbit Source: Rabbit Immunogen: A synthetic peptide corresponding to residues in the C-terminus of human calponin Immunogen: Recombinant human calretinin Specificity: Calretinin antigen Localization: Cytoplasm Specificity: Calponin Pre-treatment: AR Citra Plus/EZ-AR 1 Localization: Cytoplasm Manual/i6000: HK081-5KE Pre-treatment: AR Citra Plus/EZ-AR 1 Xmatrx: HX031-YCDE Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE Calretinin positivity in brain neurons using AEC chromogen Ready-to-use (Manual): AR413-5R AR413-10R AW413-YCD Ready-to-use (Manual): AN502-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Ready-to-use (Automated): i6000TM Xmatrx® AN502-10M AX502-YCD NU502-UC Concentrated: PU413-UP Recommended Positive Control: FG-413P Recommended Positive Control: FG-502N Calponin is a smooth muscle specific, actin-, tropomysin- and calmodulin-binding protein thought to be involved in regulation of actomyosin as well as the regulation or modulation of contraction. Immuno reactivity for Calponin, along with alpha-smooth muscle actin and smooth muscle myosin heavy chains, confirms the known neoplastic myoepithelial component of adenoid cystic carcinomas and epithelial-myoepithelial carcinomas. The consistently positive staining pattern in adenoid cystic carcinomas may be diagnostically useful in discriminating histologically similar but consistently negative polymorphous low-grade adeno carcinomas. Calretinin is approved as a highly sensitive and specific marker for mesothelial cells and one of the best positive markers for differentiating epithelial malignant mesotheliomas. This polyclonal antibody specifically recognizes calretinin in tissue originating from human, monkey, rat and mouse. It does not cross-react with other known calcium-binding proteins as determined by Western Blot analysis and by its distribution in the brain as determined by immunohistochemistry. This antibody stains calretinin antigen in cytoplasm of various neurons in normal brain and mesothelial cells. Carcinoembryonic Antigen (CEA) P Calretinin P Appendix stained with Anti-calretinin using DAB chromogen B01-94-11M-P Isotype: IgG 2b Source: Mouse Immunogen: Human carcinoembryonic antigen Carcinoembryonic antigen (CEA) Clone: 2 E7 Isotype: IgG Source: Mouse Immunogen: Recombinant human calretinin Specificity: Specificity: Anti-human calretinin Localization: Cytoplasm Localization: Cytoplasm Pre-treatment: None Pre-treatment: AR Citra Plus/EZ-AR 1 Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Recommended Positive Control: AM583-5M AM583-10M AX583-YCD MU583-UC FG-583M Calretinin, also know as calbindin 2, is a calcium binding protein that belongs to the calbindin family. It is mainly expressed in the central and peripheral nervous systems and in many normal and pathological tissues. Calretinin can be found in different subsets of neurons and is considered as a valuable marker of neuronal subpopulations for anatomical and developmental studies. It has been implicated as a calcium sensor, and regulator of apoptosis. Calretinin is approved as a highly sensitive and specific marker for mesothelial cells and one of the best positive makers for differentiating epithelial malignant mesotheliomas. 60 Clone: P - Tissue Type FFPE F - Tissue Type Frozen Colon carcinoma stained with AntiCEA using AEC chromogen Ready-to-use (Manual): AM009-5M Ready-to-use (Automated): i6000TM Xmatrx® AM009-10M AX009-YCD Concentrated: MU009-UC Recommended Positive Control: FG-009M CEA consists of a heterogeneous family of related oncofetal 200 kD glycoproteins that is secreted into the glycocalyx surface of gastrointestinal cells. Usually CEA is demonstrated as a linear labeling of the apical poles of cells lining the glandular lumen and, occasionally, as weak staining near the apex of colonic epithelial cells. Pancreatic carcinomas, testicular tumor, gall bladder neoplasms and granular cell myoblastomas stain positive, whereas malignant tumors of brain, prostate, skin, lymphoreticular tissues, hepatocellular carcinomas, esophageal squamous cell carcinomas, and mesothelioma fail to stain for CEA. This antibody stains carcinoembryonic antigen in the cytoplasm of positive cells. www.biogenex.com Primary Antibodies Carcinoembryonic Antigen (CEA) Catenin (p120) P P Clone: CEA88 Clone: YE372 Isotype: IgG1 Isotype: IgG Source: Mouse Source: Rabbit Immunogen: Partially purified human CEA Immunogen: Specificity: Carcinoembryonic antigen (CEA) Localization: Cytoplasm Pre-treatment: None A synthetic peptide corresponding to residues near Nterminusof of human Catenin (p120) was used as an immunogen. Specificity: Catenin (p120) Breast carcinoma stained with Anti-p120 Catenin using DAB chromogen Colon showing CEA positivity stained using DAB chromogen Ready-to-use (Manual): AM365-5M Ready-to-use (Automated): i6000TM Xmatrx® AM365-10M AX365-YCD Concentrated: MU365-UC Recommended Positive Control: FG-365M Recommended Barrier Control: FB-365M CEA is demonstrated as a linear labeling of the apical poles of cells lining the glandular lumen and, occasionally, as weak staining near the apex of colonic epithelial cells. CEA, however, should not be used as a marker of differentiation because many colon and lung tumors actually show increased staining with differentiation. Pancreatic carcinomas, testicular tumor, gall bladder neoplasms and granular cell myoblastomas stain positive, whereas malignant tumors of brain, prostate, skin, lymphoreticular tissues, hepatocellular carcinomas, oesophageal squamous cell carcinomas, and mesothelioma fail to stain for CEA. Cytoplasm/Membrane Pre-treatment: EZ-AR 1/EZ-AR 2 Manual/i6000: HK521-XAK Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): AN523-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN523-10M AX523-YCD NU523-UC Recommended Positive Control: FG-523N Catenins are proteins that are linked to the cytoplasmic domain of transmembrane cadherins. Catenin (p120) is one member of this Armadillo gene family of junctional plaque proteins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be important for cadherins cell-adhesion properties. Recently it became clear that some catenins also mediate nuclear signaling. Catenin (p120) is implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Catenin Delta 1 (p120) Carcinoembryonic Antigen (CEA) P Clone: Polyclonal Clone: Polyclonal Isotype: IgG Isotype: N/A Source: Rabbit Source: Rabbit Immunogen: Catenin delta CEA isolated from secondary colon carcinoma by salt precipitation, ion and gel chromatography Specificity: Catenin delta Localization: Membrane and cytoplasm Pre-treatment: Citra Plus/EZ-AR 1 Carcinoembryonic antigen (CEA) Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE P Immunogen: Specificity: CEA expression in hepatocellular carcinoma stained using DAB chromogen Localization: Localization: Cytoplasm Pre-treatment: EZ-AR 1 Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Recommended Positive Control: Breast Ca. stained with anti-Catenin delta 1 (p120) antibody using DAB chromogen AR009-5R FG-009M AW009-YCD FG-009P CEA consists of a heterogeneous family of related oncofetal 200 kD glycoproteins that is secreted into the glycocalyx surface of gastrointestinal cells. Usually CEA is demonstrated as a linear labeling of the apical poles of cells lining the glandular lumen and, occasionally, as weak staining near the apex of colonic epithelial cells. Pancreatic carcinomas, testicular tumor, gall bladder neoplasms and granular cell myoblastomas stain positive, whereas malignant tumors of brain, prostate, skin, lymphoreticular tissues, hepatocellular carcinomas, esophageal squamous cell carcinomas, and mesothelioma fail to stain for CEA. This antibody stains carcinoembryonic antigen in the cytoplasm of the positive cells. internationalcs@biogenex.com Ready-to-use (Manual): AR706-5R Ready-to-use (Automated): i6000TM Xmatrx® AR706-10R AW706-YCD Concentrated: PU706-UP Recommended Positive Control: FG-706P Recommended Barrier Control: FB-706P Catenins are proteins that are linked to the cytoplasmic domain of transmembrane cadherins. Catenin (p120) is a memberof this Aemadillo gene family of junctional plaque proteins. Cytoplasmic accumulation of Catenin (p120) has been observed in lung cancer, pancreatic cancer, gastric cancer and colon cancers and is associated with poor prognosis in colon cancer patients. In breast lobular neoplasia, anti p120 Catenin shows a diffuse cytoplasmic immunostaining pattern, while breast ductal neoplasma retains the membrane immunostaining pattern. Catenin (p120) antibody is useful in differentiation of lobular carcinoma from ductal carcinoma of the breast and in identifying early lesions of lobular neoplasia. P - Tissue Type FFPE F - Tissue Type Frozen 61 Primary Antibodies Cathepsin D CD2 P Breast carcinoma stained with AntiCathepsin D using AEC chromogen P Clone: C15 Clone: AB75 Isotype: IgG2b Isotype: IgG1 kappa Source: Mouse Source: Mouse Immunogen: Cathepsin D protein purified from human spleen Immunogen: Recombinant fusion protein corresponding to the external domain of the CD2 molecule. CD2 antigen (LFA-2) Specificity: Cathepsin D Localization: Cytoplasm Specificity: Pre-treatment: EZ-AR 1 Localization: Membrane Xmatrx: HX031-YCDE Pre-treatment: AR-10/EZ-AR2 Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Concentrated: AM467-5M AX467-YCD MU467-UC Cathepsin D production and secretion appears to be induced by estrogen in estrogen-responsive tumor cells but is constitutively produced in estrogen-unresponsive tumor cells. Immunohistochemical localization of Cathepsin D in normal human tissues has shown a granular cytoplasmic staining pattern corresponding to intracellular lysosomes. Among normal tissues studied, highest concentrations of Cathepsin D were found in sweat glands and liver with some staining of sebaceous glands. CD1a P Lymph node stained with Anti-CD1a using DAB as Chromogen Clone: O10 Isotype: IgG Source: Mouse Immunogen: Human CD1a Specificity: CD1a Localization: Membrane Pre-treatment: EZ-AR™ 1/EZ-AR 2 Manual/i6000: HK521-XAK Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): Recommended Positive Control: FG-490M CD1 is expressed on cortical thymocytes, Langerhans cells, and dendritic cells. It is absent on mature peripheral blood T cells but intracytoplasmic expression is detected on activated T lymphocytes.At least five CD1 genes (CD1a, b, c, d, and e) are identified. CD1 proteins have been demonstrated to restrict T-cell response to non-peptide lipid and lycolipid antigens and play a role in non-classical antigen presentation. Ab-5 detects cortical thymocytes, Langerhans cells in epidermis, dendritic cells of dermis and Langherhans cells of mucosa of tonsil. It may also detect small focal groups of lymphocytes outside the germinal centers of tonsil indicating a crossreaction with CD1b. This antibody is useful in the characterization of leukemias and lymphomas. P - Tissue Type FFPE F - Tissue Type Frozen HK058-5K Xmatrx: HX032-YCDE AM438-5M Ready-to-use (Automated): Xmatrx® AX438-YCD Concentrated: MU438-UC Recommended Positive Control: FG-438M The CD2 antigen, also known as lymphocyte function antigen2 (LFA2), is a single chain type I transmembrane molecule of about 50 kD and consists of 351 amino acids. It plays a critical role in activation of T cells. It binds to CD58 on antigen presenting cells and induces tyrosine phosphorylation of other molecules involved in T cell activation. It also plays a regulatory role in T-cell or NK-cell mediated cytolysis. CD2 antigen is expressed on a majority of T cells in peripheral lymphoid tissue, NK cells, cortical thymocytes and most malignant cells of T cell origin. This antibody stains membrane of positive T cells. CD3 Epsilon P Spleen tissue stained with Anti-CD3 epsilon using DAB chromogen AM490-10M AX490-YCD MU490-UC Manual: Ready-to-use (Manual): AM490-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: 62 Tonsil stained with Anti-CD2 using DAB chromogen Clone: EP449E Isotype: IgG Source: Rabbit Immunogen: A synthetic peptide corresponding to residues in cytoplasmic domain of human CD3 epsilon. Specificity: CD3 epsilon Localization: Membrane Pre-treatment: EZ-AR™ 1/EZ-AR 2 Manual/i6000: HK521-XAK Xmatrx: HX032-YCDE Ready-to-use (Manual): AN477-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN477-10M AX477-YCD Recommended Positive Control: FG-477N NU477-UC CD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C. CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labelled gamma, delta, epsilon, zeta and eta. The gamma, epsilon and delta chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). Phosphorylated ITAMS act as docking sites for protein kinases such as ZAP-70 and Syk. www.biogenex.com Primary Antibodies CD3 (T Cell) CD4 F Frozen tonsil stained with Anti-CD3 using DAB chromogen UCHT1 Clone: 4B12 Isotype: IgG1 Kappa Isotype: IgG1 Source: Mouse Source: Mouse Immunogen: Human infant thymocytes and peripheral lymphocytes from a patient with Sezary cell leukemia Immunogen: Prokaryotic recombinant protein corresponding to the external domain of the CD4 molecule Specificity: CD4 protein Specificity: CD3 Localization: Membrane Localization: Membrane Pre-treatment: AR-10/EZ-AR 1 Pre-treatment: EZ-AR 1 Manual/i6000: HK058-5K Xmatrx: HX031-YCDE Xmatrx: HX031-YCDE Ready-to-use (Manual): AM258-5M Ready-to-use (Automated): Xmatrx® Concentrated: AX258-YCD MU258-UC Tonsil stained with Anti-CD4 using DAB chromogen CD3 is a lineage-specific “pan T-cell” surface antigen composed of five different polypeptide chains with molecular masses ranging from 16 to 28 kD. Antibody UCHT1 reacts with the 20 kD epsilon chain of the CD3 molecule. CD3 is normally present on mature thymocytes, resting and activated peripheral T lymphocytes (both inducer and suppressor/ cytotoxic), and on some natural killer cells. It is absent in peripheral B lymphocytes, monocytes, granulocytes, and platelets. This antibody stains CD3 antigen in the cytoplasm of immature and common thymocytes and on the surface of mature thymocytes in frozen tissue sections. CD3 (T Cell) P Tonsil stained with Anti-CD3 using DAB chromogen P Clone: Ready-to-use (Manual): AM421-5M Ready-to-use (Automated): i6000TM Xmatrx® AM421-10M AX421-YCD Concentrated: MU421-UC Recommended Positive Control: FG-421M Recommended Barrier Control: FB-421M CD4 is a 55-60 kD cell-surface glycoprotein, which participates in the molecular complexes involved in both T cell development and its antigen recognizing activity, by binding to the nonpolymorphic region of class II MHC. CD4 is considered as a stage marker of T cell development in the thymus, for it is expressed on the cell surface in a stage specific manner, during T cell development. This antibody reacts on a low level with human monocytes and macrophages but does not react with B-cells, granulocytes and thrombocytes. This antibody stains CD4 antigen on the membrane of positive T lymphocytes. Clone: PS1 Isotype: IgG 2a Source: Mouse Immunogen: Fusion protein to the epsilon chain of CD3 Specificity: CD3 antigen Clone: EP204 Localization: Membrane Isotype: IgG Pre-treatment: AR-10/EZ-AR 1 Source: Rabbit Manual/i6000: HK058-5K Immunogen: Xmatrx: HX031-YCDE A synthetic peptide corresponding to residues of human CD4 protein Specificity: CD4 protein Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Recommended Positive Control: Recommended Barrier Control: CD4 AM322-5M AM322-10M AX322-YCD MU322-UC Tonsil stained with anti-CD4 using DAB chromogen FG-322M FB-322M Human CD3 complex is associated with the T cell receptor (TcR) at the cell surface. Expression of CD3 antigen is generally restricted to the T-cell lineage, but weak expression might also occur in Purkinje cells in the brain, in macrophages, and in Reed-Sternberg cells in Hodgkin's lymphoma. The CD3 antigen is expressed early in the maturation of T cells. Monoclonal antibody PS1 reacts with the nonglycosylated epsilon chain of CD3. The antibody stains CD3 antigen in the membrane of the positive cells. internationalcs@biogenex.com P Localization: Membrane Pre-treatment: AR-10/EZ-AR 2 Manual/i6000 HK058-5K Xmatrx HX032-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN722-5M Recommended Positive Control: AN722-10M AY722-YCD NU722-UC FG-722N Recommended Barrier Control: FB-722N CD4 (cluster of differentiation 4) is a glycoprotein found on the surface of immune cells such as T helper cells, monocytes, macrophages and dendritic cells. CD4 is expressed in the majority of T-cell lymphomas, including mycosis fungoides. Lymphomas are CD4 positive with the exception of aggressive NK-cell leukemia and extranodal NK/Tcell lymphoma. CD4 plays an important role in the classification of lymphocytes in inflammatory lesions and malignant lymphomas. P - Tissue Type FFPE F - Tissue Type Frozen 63 Primary Antibodies CD5 CD7 P Tonsil tissue stained with Anti-CD5 using DAB chromogen 4C7 Clone: LP15 Isotype: IgG1 Kappa Isotype: IgG2b Source: Mouse Source: Mouse Immunogen: Recombinant protein corresponding to the external domain of the CD5 molecule. Immunogen: CD7 Specificity: CD7 Localization: Membrane Specificity: Human CD5 antigen, 67 kD antigen Pre-treatment: Citra Plus/EZ-AR 1 Manual/i6000: HK081-5KE Localization: Membrane Pre-treatment: AR-10/EZ-AR 2 Xmatrx: HX031-YCDE Manual/i6000 HK058-5K Xmatrx: HX032-YCDE Ready-to-use (Manual): AM430-5M Ready-to-use (Automated): i6000TM Xmatrx® AM430-10M AX430-YCD Concentrated: MU430-UC Recommended Positive Control: FG-430M The CD5 antigen, also known as T1, is a 67 kD single chain glycoprotein expressed on normal and malignant T cells and on chronic lymphocytic leukemia cells. It is found in high density on medullary thymocytes and in low density on cortical thymocytes. CD5 P Lymph node stained with Anti-CD5 using AEC chromogen Clone: EP2952 Isotype: IgG Source: Rabbit Immunogen: A synthetic peptide corresponding to residues in human CD5 Tonsil stained with Anti-CD7 using DAB Chromogen Ready-to-use (Manual): AM702-5M Ready-to-use (Automated): i6000TM Xmatrx® AM702-10M AY702-YCD Concentrated: MU702-UC Recommended Positive Control: Recommended Barrier Control: FG-702M FB-702M The CD7 molecule is membrane-bound glycoprotein of 40kD and is the earliest T Cell specific antigen to be expressed in lymphocyetes. CD7 antigen is also the only early marker to persist throughout differentiation. The function and role of the CD7 molecule has not yet been fully identified although the activation of T cells with gamma/ delta receptors has been proposed based on mAb- activation. CD7 antigen is reported to be found on a majority of peripheral blood T cells, most natural killer cells and thymocytes. CD8 P Clone: 1A5 Isotype: IgG1 Specificity: CD5 Source: Mouse Localization: Membrane Immunogen: Pre-treatment: EZ-AR™ 2/EZ-AR 1 Manual/i6000: HK522-XAK Prokaryotic recombinant protein corresponding to the external domain of the CD8 molecule. Xmatrx: HX031-YCDE/HX032-YCDE Specificity: CD8 protein Ready-to-use (Manual): AN512-5M Ready-to-use (Automated): i6000TM Xmatrx® AN512-10M AX512-YCD Concentrated: NU512-UC Recommended Positive Control: FG-512N CD5 (Lymphocyte antigen T1/Leu-1) is a transmembrane glycoprotein which has been implicated as a receptor in the regulation of T-cell proliferation. CD5 is part of the scavenger receptor cysteine-rich (SRCR) family group B and it is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by T cell leukemia, lymphomas, and activated T cells. Occasionally, CD5 antigen is also expressed on a subset of B cells. Mantle cell lymphomas (same as diffuse centrocytic lymphomas) are CD5+ while the follicle center cell lymphomas are CD5. A ligand for CD72/LYB-2, CD5 has been linked to negative regulation of T cell and B cell receptors signaling. 64 P Clone: P - Tissue Type FFPE F - Tissue Type Frozen Tonsil tissue stained with Anti-CD8 using DAB chromogen Localization: Membrane Pre-treatment: AR-10/EZ-AR 2 Manual/i6000 HK058-5K Xmatrx: HX032-YCDE Ready-to-use (Manual): AM422-5M Ready-to-use (Automated): i6000TM Xmatrx® AM422-10M AX422-YCD Concentrated: MU422-UC Recommended Positive Control: FG-422M Recommended Barrier Control: FB-422M The CD8 antigen is composed of two chains and has a molecular weight of 32kD. This antibody identified cytotoxic/suppressor T cells that interact with binding to the nonpolymorphic region of class I MHC. CD8 is a stage marker of T cell development in the thymus, and is expressed on mature peripheral T cells, most cytotoxic T cells, CD4/ CD8+ thymocytes, NK cells and cortical thymocytes. This monoclonal antibody is directed against the CD8 antigen, which is expressed on human T lymphocytes. It does not react with B-cells, granulocytes and thrombocytes. This antibody stains CD8 antigen on the membrane of positive T lymphocytes. www.biogenex.com Primary Antibodies CD8 CD8 (T cell, Suppressor/Cytotoxic) P Tonsil stained with anti-CD8 using DAB F Clone: SP16 Clone: T8 Isotype: IgG Isotype: IgG1 Kappa Source: Rabbit Source: Mouse Immunogen: Synthetic peptide corresponding to C-terminus of alpha chain of the human CD8 molecule Specificity: CD8 Localization: Membrane Pre-treatment: AR Citra Plus/EZ-AR 2 Manual/i6000: HK081-5KE Xmatrx: HX032-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Immunogen: Human thymocytes Specificity: CD8 antigen Localization: Membrane Pre-treatment: EZ-AR 1/EZ-AR 2 Xmatrx: HX031-YCDE/HX032-YCDE Frozen tonsil stained with Anti-CD8 using AEC chromogen AN740-5M AN740-10M AY740-YCD NU740-UC The CD8 molecule consists of two chains, α and β, which are expressed as a disulphide-linked α/β heterodimer or as an α/α homodimer on a T cell subset (25-35% of mature peripheral T-cells), thymocytes (7080%), and NK cells (30%, which are also CD3 negative). The majority of CD8+ T cells express CD8 as α/β heterodimer. CD8 functions as a coreceptor in concert with TCR for binding the MHC class I/peptide complex. The HIV-2 envelope glycoprotein binds CD8 α chain (but not β chain). Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® AM261-5M AX261-YCD Concentrated: MU261-UC The CD8 antigen, also known as T8 or Leu2 or Lyt2 or T cell coreceptor, is a dimer with a molecular mass of 32 kD. The T8 antigen is expressed by the suppressor/cytotoxic subset of T lymphocytes which comprise most of the cortical thymocytes and approximately 30% of peripheral blood T cells. Studies have demonstrated that increased levels of T8+ cells are associated with viral infections such as hepatitis B, Epstein-Barr, and cytomegalovirus. This antibody may be used in the study of cell-mediated cytotoxicity and that of immunoregulation and T-lymphocyte-mediated suppression. This antibody stains CD8 (T8) antigen suppressor/cytotoxic T lymphocytes and majority of thymocytes (approximately 80%) in frozen tissue sections. CD10 P CD8 (C-term) P Lymph node stained with Anti-CD8 using AEC chromogen Clone: EP1150Y Isotype: IgG Source: Rabbit Immunogen: A synthetic peptide corresponding to residues near the Cterminus of human CD8. Specificity: CD8 ( C-term) Localization: Membrane Pre-treatment: AR Citra Plus /EZ-AR 1/ EZ-AR 2 Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): AN513-5M Ready-to-use (Automated): i6000TM Xmatrx® AN513-10M AX513-YCD Concentrated: NU513-UC Recommended Positive Control: FG-513N CD10 expression in kidney stained using DAB chromogen The T cell coreceptor CD8 is a cell-surface glycoprotein expressed either as a disulfide-linked homodimer of two CD8 alpha monomers, or a heterodimer of CD8 alpha and CD8 beta. These receptors interact with ligands, such as major histocompatibility complex (MHC) class I, on the outside of the cell, with proteins inside the cell, such as the tyrosine kinase p56lck, and possibly with proteins on the same cellsurface. CD8 plays an important role in T cell development in the thymus and T cell activation in the periphery. internationalcs@biogenex.com Clone: 56C6 Isotype: IgG1 Source: Mouse Immunogen: Human CD10 Specificity: CD10 Localization: Membrane Pre-treatment: EZ-ARTM 1//EZ-AR 2 Manual/i6000: HK521-XAK Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): AM451-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM451-10M AX451-YCD MU451-UC Recommended Positive Control: FG-451M CD10, a 100KD glycoprotein, also known as Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt’s and follicular germinal center lymphomas, and chronic myelogenous leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, with especially high expression on the brush border of kidney and gut epithelial cells. P - Tissue Type FFPE F - Tissue Type Frozen 65 Primary Antibodies CD11b (C3bi receptor) F CD11C Clone: EP1347Y Isotype: IgM kappa Isotype: IgG Source: Mouse Source: Rabbit Immunogen: A synthetic peptide corresponding to residues onthe C-terminus of human CD11c/ITGAX CD11c Immunogen: Frozen tonsil mucosa stained with Anti-CD11b using DAB chromogen Human adherent mononuclear cells Specificity: Myeloid antigen M01 Localization: Membrane Pre-treatment: EZ-AR 1/EZ-AR 2 Specificity: HX031-YCDE/HX032-YCDE Localization: Membrane Pre-treatment: AR Citra Plus/ /EZ-AR 1/ EZ-AR 2 Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE/HX032-YCDE Xmatrx: Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® AX270-YCD P Spleen tissue stained with Anti-CD11bITAM using DAB as chromogen Tonsil tissue stained with AntiCD11c using DAB as chromogen AM270-5M CD11b also known as M01, a human myeloid antigen, is a noncovalently associated, two-subunit glycoprotein of 94 and 155 kD. This antibody can be used as a neutrophil marker. It identifies the C3bi receptor and is expressed by peripheral blood monocytes, certain macrophages, granulocytic cells (from myelocytes to mature neutrophils) and a subset of null cells in frozen tissue sections. Histiocytic cells in the spleen and lymph nodes also express this antigen. CD11b/ITAM P M01 Clone: Clone: EPR1344 Isotype: IgG Source: Rabbit Immunogen: A synthetic peptide corresponding to residues in human CD11b . Ready-to-use (Manual): AN555-5M Ready-to-use (Automated): i6000TM Xmatrx® AN555-10M AX555-YCD Concentrated: Recommended Positive Control: NU555-UC FG-555N CD11c (integrin alpha chain protein) combines with the beta 2 chain to form a leukocyte specific integrin. The integrin CD11c/CD18 functions as a cell surface receptor for numerous soluble factors and proteins (LPS, fibrinogen, iC3b), mediates leukocyte interactions with other cell types and is a signal transducing receptor. CD11c/ CD18 is found primarily on myeloid cells, where its expression is regulated both during differentiation and during monocyte maturation into tissue macrophages. The CD11c antigen is a useful marker for hairy cell leukemia and in its differentiation from other leukemias and lymphomas. Specificity: CD11b/ITAM Localization: Membrane Pre-treatment: EZ-AR™ 1 Clone: EPR3653 Manual/i6000: HK521-XAK Isotype: IgG Xmatrx: HX031-YCDE Source: Rabbit Immunogen: A synthetic peptide corresponding to residues in human CD14 Specificity: CD14 Ready-to-use (Manual): AN546-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN546-10M AX546-YCD NU546-UC Recommended Positive Control: FG-546N Integrin alpha-M (ITAM, ITGAM, CD11b, MAC-1 alpha subunit, CR3 alpha chain) belongs to the integrin alpha chain family and it is predominately presented in human myeloid cells, NK1 cells, monocytes, and granulocytes. The alpha subunit of ITAM/beta-2 complex (CD11b/CD18, Mac-1), is a receptor for fibrinogen (binding to P1 and P1 peptides of gamma chain), factor X, and ICAM1. ITAM/beta2 is implicated in adhesive interactions of monocytes, macrophages, and granulocytes. It is also linked to mediation of complement-coated particle uptake. CD14 P Placental tissue stained with AntiCD14 using AEC chromogen Localization: Cytoplasm/Membrane Pre-treatment: EZ-AR™ 1 Manual/i6000: HK521-XAK Xmatrx: HX031-YCDE Ready-to-use (Manual): AN515-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN515-10M AX515-YCD NU515-UC Recommended Positive Control: FG-515N CD14 is a glycophosphatidyl inositol-anchored protein expressed on the surfaces of monocytes and polymorphonuclear leukocytes. It acts as a co-receptor for the detection of bacterial lipopolysaccharide (LPS). CD14 cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial LPS. It acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. CD14 is expressed strongly on peripheral blood monocytes and macrophages, and weakly on the surface of granulocytes. 66 P - Tissue Type FFPE F - Tissue Type Frozen www.biogenex.com Primary Antibodies CD15 (Blood Group Antigen Lewis X) CD19 P BRA4F1 Clone: EP169 Isotype: IgM Isotype: IgG Source: Mouse Source: Rabbit Immunogen: Myelomonocytic leukemia cells Immunogen: A recombinant fragment corresponding to residues in human CD19 protein Specificity: Human CD19 protein Specificity: CD15 Localization: Membrane/Cytoplasm/ perinuclear structures Pre-treatment: None Tonsil stained with anti-CD19 using DAB chromogen Bone marrow stained with AntiCD15 using DAB chromogen Ready-to-use (Manual): AM302-5M Ready-to-use (Automated): i6000TM Xmatrx® AM302-10M AX302-YCD Concentrated: MU302-UC Recommended Positive Control: FG-302M Recommended Barrier Control: FB-302M CD16 Lymph node stained with Anti-CD16 using AEC chromogen Localization: Membrane Pre-treatment: AR Citra Plus/EZ-AR 1 Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: CD15 (BRA4F1) reacts with human CD15 antigen present on myeloid cells, mainly granulocytes but not on B cells, T cells, monocytes, erythrocytes or platelets. It also reacts with Hodgkin's and ReedSternberg cells in individuals with Hodgkin's disease. This antibody stains CD15 antigen in positive cells. P P Clone: AN729-5M Recommended Positive Control: AN729-10M AY729-YCD NU729-UC FG-729N Recommended Barrier Control: FB-729N CD19 is expressed on follicular dendritic cells and B cells. In fact, it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. CD19 has been observed in lymphomas and leukemias but often weak/negative in follicular lymphoma or diffuse large B-cell lymphoma. CD19 may provide useful diagnostic information for the study of B-lymphoproliferative disorders. Clone: 2H7 Isotype: IgG2a Source: Mouse Clone: CD20/C23 Immunogen: Recombinant fusion protein corresponding to the external domain of the CD16 molecule common to both the transmembrane form and the GPI-linked form Isotype: IgG1 kappa Source: Mouse Localization: Membrane Specificity: CD16 antigen Pre-treatment: Localization: Membrane & Cytoplasm AR Citra Plus/ /EZ-AR 1/ EZ-AR 2 Pre-treatment: AR-10/EZ-AR 2 Manual/i6000: HK081-5KE Manual: HK058-5K Xmatrx HX031-YCDE/HX032-YCDE Xmatrx: CD20 Tonsil stained with Anti-CD20 using DAB chromogen HX032-YCDE Immunogen: Human CD20 Specificity: CD20 Ready-to-use (Manual): AM537-5M AX437-YCD MU437-UC Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM537-10M AX537-YCD FG-437M Recommended Positive Control: Ready-to-use (Manual): AM437-5M Ready-to-use (Automated): Xmatrx® Concentrated: Recommended Positive Control: CD16 antigen is also known as Fc gamma receptor III and has a molecular weight of 50 to 70kD. It is a low affinity Fc receptor for complexed IgG-Fc gamma RIII, expressed on natural killer (NK) cells, granulocytes, activated macrophages and a subset of T cells expressing alpha-beta or gamma-delta T cell antigen receptors. Antibody-dependent cytotoxicity of NK cells is triggered by the engagement of CD16 with the Fc portion of IgG immunoglobulins bound to target cell-associated antigens. This antibody may be useful in the study of NK cell activity in autoimmune, neoplastic and infectious diseases. This antibody stains membrane and in the cytoplasm of positive cells. internationalcs@biogenex.com P MU537-UC FG-537M CD20 is a transmembrane, non-glycosylated protein expressed on B-cell precursors and mature B cells, but is lost following differentiation into plasma cells. This antibody does not cross-react with nonhematopoietic neoplasms. CD20 (B-cell Pan) reacts with a membrane antigen present in B-cells. This antibody strongly recognizes ReedSternberg cells predominant in Hodgkin`s disease. Since no staining of histiocytes or plasma cells has been observed and CD20 has not been detected in T-cell malignancies, it is a very strong marker of B-cell lymphomas. B-cell panmarker recognizes a formalin resistant intracytoplasmic antigen. P - Tissue Type FFPE F - Tissue Type Frozen 67 Primary Antibodies CD20 (B Cell) CD21 P P L-26 Clone: EP3093 Isotype: IgG2a Kappa Isotype: IgG Source: Mouse Source: Rabbit Immunogen: A synthetic peptide corresponding to residues on the C terminus of human CD21. Specificity: CD21 Clone: Immunogen: Human tonsil B cells Specificity: CD20 Localization: Membrane Pre-treatment: None Tonsil tissue stained with Anti-CD21 using AEC chromogen Tonsil tissue stained with Anti-CD-20 using DAB chromogen Localization: Membrane Pre-treatment: EZ-AR™ 2 Manual/i6000: HK522-XAK Xmatrx: HX031-YCDE/HX032-YCDE AM238-5M Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® AM238-10M AX238-YCD Concentrated: MU238A-UC Recommended Positive Control: FG-238M Recommended Barrier Control: FB-238M This antibody reacts with a formalin-resistant intracytoplasmic epitope found in the majority of B cells which is now considered to be the CD20 antigen, a pan-B cell marker. The antibody primarily recognizes a 33 kD polypeptide B cell component and also a minor 30 kD cellular antigen.The staining pattern is consistent with pan-B reactivity, producing staining for B cells in lymphoid and peripheral blood tissue. This antibody intensely stains germinal centers and B immunoblasts in lymphoid tissue. L26 may prove to be a useful marker for L&H variants of Reed-Sternberg cells of Hodgkin's lymphomas where reactive pattern is distinct from other Reed-Sternberg variants. This antibody stains positive for membrane and some cytoplasm for B cells. Ready-to-use (Manual): AN497-5M Ready-to-use (Automated): i6000TM Xmatrx® AN497-10M AX497-YCD Concentrated: Recommended Positive Control: NU497-UC FG-497N CD21 is a transmembrane protein that serves as the complement receptor C3d and the Epstein-Barr virus, also known as CR2. CD21 is found on human B cells. CD21 interacts with the p53 anti-oncoprotein, the p68 calcium binding protein and the nuclear p120 ribonucleoprotein to regulate their functions. Phosphorylation of CD21 inhibits its interaction with the nuclear p120 ribonucleoprotein. The soluble CD21 (sCD21) was shown to efficiently trigger CD23 signaling pathways in human monocytes. By inducing release of proinflammatory cytokines and upregulating expression of molecules involved in antigen presentation, sCD21 modulates critical monocyte functions that may be relevant to allergic and inflammatory disorders. CD21 (B Cell) F CD20 (B Cell) F IgM Kappa B1 Source: Mouse Isotype: IgG2 Kappa Immunogen: Source: Mouse Immunogen: Human B cell ascites Burkitt's lymphoma cells Human diffuse poorly differentiated lymphoma cells from a patient with B cell lymphoma B2+ B lymphocytes Specificity: B1+ B cells Specificity: Localization: Membrane Localization: Membrane Pre-treatment: EZ-AR 1/EZ-AR 2 Pre-treatment: EZ-AR 1/EZ-AR 2 Xmatrx: HX031-YCDE/HX032-YCDE Xmatrx: HX031-YCDE/HX032-YCDE AM265-5M AX265-YCD MU265-UC The CD20 antigen, also known as B1, is a 33-35 kD glycoprotein found on all B cells in peripheral blood, lymph node, spleen, tonsil, and bone marrow. It is expressed in 50% of CALLA-positive acute lymphoblastic leukemias (ALL), but not on normal T lymphocytes, monocytes, granulocytes, or tumors derived from these cell lineages. Aside from B cells, other normal cells do not express the CD20 antigen. This antibody may be used in the study of normal and neoplastic cells of B origin, in the study of B cell development and activation, and in the classification of acute leukemias. This antibody stains CD20 (B1) antigen on membranes of all B cells in peripheral blood, lymph node, spleen, tonsil, and bone marrow in frozen tissue sections. 68 B2 Isotype: Clone: Frozen tonsil stained with Anti-CD20 using AEC chromogen Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Concentrated: Clone: P - Tissue Type FFPE F - Tissue Type Frozen Frozen tonsil stained with Anti-CD21 using AEC chromogen Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® AM266-5M AX266-YCD The B2 antigen, also known as CD21 and CR2, is a 140 kD glycosylated Type 1 integral membrane protein. The CD21 molecule is expressed on mature B lymphocytes, follicular dendritic reticulum cells (FDC), pharyngeal epithelial cells, and possibly on a subset of normal thymocytes. This antigen is also expressed by B lymphocytes in patients with B cell lymphomas, most B cell chronic lymphocytic leukemia (CLL), and a small portion of non-T cell acute lymphoblastic leukemias (ALL). This antibody stains B2 (CD21) antigen in membranes of mature B lymphocytes, follicular dendritic reticulum cells (FDC), pharyngeal epithelial cells, and possibly on a subset of normal thymocytes in frozen tissue sections. www.biogenex.com Primary Antibodies CD22 CD25 P Tonsil stained with anti-CD22 using DAB chromogen FPC1 Clone: 4C9 Isotype: IgG1 Isotype: IgG1 Source: Mouse Source: Mouse Immunogen: Recombinant fusion protein corresponding to the external domain of the CD22 molecule Immunogen: Human CD25 Specificity: CD25 Localization: Membrane Specificity: Human CD22 antigen (BL-CAM) Pre-treatment: EZ-ARTM 1/EZ-AR 2 Manual/i6000: HK521-XAK Localization: Membrane Xmatrx: HX031-YCDE/HX032-YCDE Pre-treatment: AR-10/EZ-AR 1 Manual: HK058-5K Xmatrx: HX031-YCDE Ready-to-use (Manual): AM439-5M Ready-to-use (Automated): Xmatrx® AX439-YCD Concentrated: MU439-UC Recommended Positive Control: FG-439M Tonsil stained with Anti-CD25 using DAB chromogen The CD22 antigen also known as BL-CAM is a single chain type I transmembrane molecule which contains seven Ig-like domains and molecular weight of 130 to 140kD. In B-cell malignancies, CD22 expression ranges from 60% to 80% depending on the histological type and on the assays used. CD22 antigen is weakly expressed in myeloid leukemias and non-T cell acute lymphoblastic leukemias and is strongly expressed in hairy cell leukemias. It is absent on peripheral blood T cells, T cell leukemias, granulocytes, and monocytes. This antibody stains both membrane and cytoplasm of B lymphocytes. CD23 P Clone: Ready-to-use (Manual): AM453-5M Ready-to-use (Automated): i6000TM Xmatrx® AM453-10M AX453-YCD Concentrated: MU453-UC Recommended Positive Control: FG-453M CD25 antigen is an alpha subunit of the interleukin-2 receptor. It is expressed on a subpopulation of T cells subsequent to their activation with an antigen or mitogen in the presence of interleukin-1. CD25 is also found on HTLV-1 transformed T and B cells, EBV transformed B cells, myeloid precursors and oligodendrocytes. This antibody may be useful in studies of inflammation, lymphoid neoplasia, autoimmune diseases or allograft rejection to help characterize CD25 expression. CD29 (Integrin Beta-1 Subunit) P P CD23 positivity in Lymph node stained using DAB chromogen Clone: Polyclonal Isotype: IgG Clone: JB1a Isotype: IgG Source: Mouse Immunogen: Purified ß 1 integrin from Jurkat cells CD29 Source: Rabbit Specificity: Immunogen: CD23 antigen Localization: Membrane Specificity: CD23 Pre-treatment: AR Citra/EZ-AR 2 Localization: Membrane Pre-treatment: EZ-ARTM 1 Manual/i6000: HK521-XAK Xmatrx: HX031-YCDE Ready-to-use (Manual): AR460-5R Ready-to-use (Automated): i6000TM Xmatrx® AR460-10R AW460-YCD Concentrated: Recommended Positive Control: Breast carcinoma expressing CD 29 stained using DAB chromogen PU460-UP FG-460P CD23 is a 45 kD type II integral membrane glycoprotein that belongs to the C-type lectin family of adhesion molecules.The CD23 molecule is identical to the low affinity IgE receptor found on B-cells. CD23 has been proposed to be an important regulator of IgE synthesis. AntiCD23 antibody treatment of rats inhibited antigen-specific IgE immune response by 90%. CD23 is a common B cell/monocyte surface antigen. CD23 is expressed on IgM+/IgD+ B cells, as well as on a variety of other cells, including monocytes, eosinophils, dendritic cells, platelets, and macrophages. Expression of CD23 has been detected in neoplastic cells such as chronic lymphocytic leukemia, some cases of lymphoma and is strongly expressed on EBV transformed B lymphoblasts. internationalcs@biogenex.com Manual HK087-5KE Xmatrx: HX032-YCDE Ready-to-use (Manual): AM298-5M Ready-to-use (Automated): Xmatrx® Concentrated: AX298-YCD MU298-UC Recommended Positive Control: FG-298M Integrins play an important role in cell adhesion and migration, and their normal function is critical in the induction and maintenance of cell differentiation. This antibody reacts with CD29, the 130 kD integrin ß1 subunit. CD29 is ubiquitous, with broad tissue distribution, but is not expressed on erythrocytes and is expressed only weakly on granulocytes. Loss or down-regulation of CD29 has been proposed to be one of the general pathways through which carcinoma cells may acquire a more invasive and differentiated phenotype. This antibody stains CD29 antigen in cell membrane of most cells including all leukocytes, although very weak on granulocytes. P - Tissue Type FFPE F - Tissue Type Frozen 69 Primary Antibodies CD30 (Ki-1 Antigen) CD31 (Endothelial Cell) P Hodgkins stained with Anti-CD30 using AEC chromogen Clone: Ber-H2 Isotype: IgG1 Kappa Spleen membrane from a patient with hairy cell leukemia CD31 antigen CD30 (Ki-1) antigen Localization: Membrane & Cytoplasm Specificity: Pre-treatment: AR-10/EZ-AR 1 Localization: Membrane & Cytoplasm Manual/i6000: HK058-5K Pre-treatment: Xmatrx: HX031-YCDE Pepsin, 370C for 5 min./ EZ-AR 2 Manual: EK000-5K Xmatrx: HX031-YCDE FG-327M CD30 (Ki-1 antigen), a 120 kD single chain glycoprotein, is expressed in only a small population of normal lymphoid tissue. By contrast, it is expressed in approximately 50% of all malignant lymphomas including all cases of Hodgkin's disease and a vast majority of Ki-1 positive anaplastic large cell lymphomas. Ki-1 antigen can be detected in sera from lymphoma patients, but not in sera from normal individuals with systemic infection. This antibody stains CD30 (Ki-1) antigen in the membrane of positive cells. CD30 (Ki-1 Antigen) Clone: HRS-4 Isotype: IgG1 Source: Mouse Immunogen: Cell line L540, derived from Hodgkin's disease Specificity: CD30 (Ki-1 antigen) Localization: Membrane (mostly perinuclear dot positivity) & Cytoplasm Pre-treatment: AR Citra Plus/EZ-AR 1 Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE AM351-5M AM351-10M AX351-YCD MU351-UC CD30 (Ki-1 antigen), a 120 kD single chain glycoprotein, is expressed in only a small population of normal lymphoid tissue. By contrast, it is expressed in approximately 50% of all malignant lymphomas including all cases of Hodgkin's disease and a vast majority of Ki-1 positive anaplastic large cell lymphomas. Ki-1 antigen can be detected in sera from lymphoma patients, but not in sera from normal individuals with systemic infection. This antibody stains CD30 antigen in membrane and sometimes cytoplasm of the positive cells. 70 Mouse Immunogen: Specificity: Recommended Positive Control: Concentrated: Source: Mouse AM327-10M AX327-YCD Ready-to-use (Manual): IgG1 Kappa Hodgkin's lymphoma cell line L428 Ready-to-use (Automated): i6000TM Xmatrx® Ready-to-use (Automated): i6000TM Xmatrx® JC/70A Isotype: Immunogen: AM327-5M Reed Sternberg cell showing perinuclear dot positivity of CD30 antibody stained using DAB chromogen Clone: Source: Ready-to-use (Manual): P P P - Tissue Type FFPE F - Tissue Type Frozen Endothelial cells stained with AntiCD31using DAB chromogen Ready-to-use (Manual): AM232-5M Ready-to-use (Automated): i6000TM Xmatrx® AM232-10M AX232-YCD Recommended Positive Control: FG-232M Anti-CD31 monoclonal antibody JC/70A reacts with a membrane glycoprotein with an apparent size of 100 kD in endothelial cells and 130 kD in platelets. It strongly stains endothelium in normal tissue as well as benign and malignant tumor tissue. The antibody labels megakaryocytes, platelets, and occasionally plasma cells, and weakly stains mantle zone B cells, peripheral T cells and neutrophils. This antibody stains CD31 antigen in membrane and sometimes cytoplasm of endothelial and other positive cells in normal and abnormal tissues. CD31 (PECAM-1) P Lymph node stained with AntiPECAM-1 using AEC chromogen Clone: 9G11 Isotype: IgG1 Source: Mouse Immunogen: Activated human umbilical vein endothelial cells Specificity: CD31 Localization: Membrane & Cytoplasm Pre-treatment: AR-10/EZ-AR 1/EZ-AR 2 Manual: HK058-5K Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): AM241-5M Ready-to-use (Automated): Xmatrx® Concentrated: AX241-YCD Recommended Positive Control: FG-241M MU241-UC CD31 (Platelet Endothelial Cell Adhesion Molecule) is a 145 kD cell surface glycoprotein that was originally defined by a monoclonal antibody which is bound to endothelial cells and also to platelets. This protein may be a component involved in the interaction of endothelial cells with coagulation factors, platelets, and the subendothelial matrix. The antibody has been shown to be specific for CD31 and reacts mainly with platelets, monocytes, macrophages, granulocytes, and B cells. The other reactive cells are endothelial cells, histiocytes, and glomeruli. This antibody stains CD31 antigen in membrane of endothelial cells and other positive cells. www.biogenex.com Primary Antibodies CD34 (Endothelial Cell) P CD35 P Clone: QBEnd/10 Clone: SP191 Isotype: IgG1 Isotype: IgG Source: Mouse Source: Rabbit Immunogen: CD34 isolated from human placental endothelial cells Immunogen: Synthetic peptide near from the C-terminus of human CD35 protein Specificity: CD34 Specificity: CD35 Localization: Membrane Localization: Membrane Pre-treatment: None Pre-treatment: EZ-AR™ 2 Tonsil stained with anti-CD35 using DAB Endothelial cells stained with AntiCD34 using DAB chromogen Ready-to-use (Manual): AM236-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM236-10M AX236-YCD MU236-UC Recommended Positive Control: FG-236M Recommended Barrier Control: FB-236M Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: This is an antibody to the CD34 antigen in human endothelial and hematopoietic cells. It stains positive in a variety of vascular and lymphatic tumors. QBEnd/10 may now prove to be a more specific method of evaluating vascularization than Factor VIII antibody and is an important tool for tumor evaluation. This antibody stains endothelial cell cytoplasm and cross-reacts with basement membrane collagen. AN741-5M AN741-10M AY741-YCD NU741-UC CD35, also named as erythrocyte complement receptor 1 (CR1), is a member of the complement activation (RCA) family and is located in the ‘cluster RCA’ region of chromosome 1. CD35 expressed by glomerular podocytes, erythrocytes, and leukocytes (B cells, subset of T cells, monocytes, macrophages, neutrophils, and eosinophils). CD35 also can be detected on follicular dendritic cells. It is a marker for the diagnosis of follicular dendritic cell sarcoma. This antibody labels dendritic cells in tonsil and spleen and glomerular podocytes in kidney. CD41/Integrin Alpha IIb CD35 P Clone: EP178 Clone: RLB25 Isotype: IgG Isotype: IgG2b Source: Rabbit Source: Mouse Immunogen: Immunogen: Prokaryotic recombinant fusion protein corresponding to the first four complement control protein domains of the CD35 molecule A synthetic peptide corresponding to residues of human CD41/ Integrin alpha IIb protein Specificity: CD41/Integrin alpha IIb P Tonsil stained with Anti-CD35 using DAB chromogen Specificity: CD35 Localization: Membrane Pre-treatment: HK058-5K Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): AM431-5M Ready-to-use (Automated): i6000TM Xmatrx® AM431-10M AX431-YCD Concentrated: MU431-UC Recommended Positive Control: FG-431M The CD35 antigen is a transmembrane monomeric glycoprotein of 60-250kD. It is also known as complement receptor 1 (CR1) or C3b/ C4b receptor as it binds the complement components C3b and C4b and thereby helps clear foreign particles. By facilitating C3b and C4b cleavage by factor I and accelerating the decay of the C3 and C5 convertases, CD35 limits complement activation and produces ligands for other complement receptors. CD35 antigen has been used in characterization of tumors of histiocytes and accessory dendritic cells by immunohistochemistry. This antibody stains the membrane of follicular dendritic cells, a subset of T- cells. Membrane/Cytoplasm Pre-treatment: AR-10 Manual/i6000: HK058-5K Xmatrx AR-10/EZ-AR 1/EZ-AR 2 Manual/i6000: internationalcs@biogenex.com Spleen stained with anti-CD41 using DAB chromogen Localization: Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: None AN732-5ME Recommended Positive Control: AN732-10ME AY732-YCDE NU732-UCE FG-732N Recommended Barrier Control: FB-732N Integrin alpha chain 2b, also known as CD41, is a heterodimeric integral membrane protein. CD41 is expressed on platelets and megakaryocytes, but also on early embryonic hematopoietic stem cells. The integrin alpha chain associates with a beta 3 chain, CD61. The resulting CD41/CD61 complex is a receptor for fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin, and has a crucial role in coagulation. Mutations that impair its role in coagulation result in thrombasthenia. P - Tissue Type FFPE F - Tissue Type Frozen 71 Primary Antibodies CD43 & CD45RA Cocktail CD44 (Phagocytic Glycoprotein-1, HCAM) P P Clone: MT1 & MB1 Clone: DF1485 Isotype: IgG1 Isotype: IgG2b Source: Mouse Source: Mouse Immunogen: Hodgkin’s lymphoma Immunogen: Specificity: Leukocyte Cell surface glycoprotein CD44 Localization: Membrane Pre-treatment: EZ-AR 1/EZ-AR 2 Xmatrx: HX031-YCDE/HX032-YCDE Breast Tissue stained with AntiCD44 using DAB chromogen Tonsil stained with Anti-CD43 & Anti-CD45RA cocktail using AEC chromogen Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Recommended Positive Control: Localization: Membrane Pre-treatment: AR Citra Manual: HK087-5KE Xmatrx: None Ready-to-use (Manual): AM310-5M AX159-YCD Ready-to-use (Automated): Xmatrx® AX310-YCD FG-159M Concentrated: MU310-UC Recommended Positive Control: FG-310M AM159-5M MB1 recognizes a membrane-bound antigen which occurs on all B cells with the exception of plasma cells, and on some mature T cells. The MB1 antigen is not present on immature T cells. MT1 reacts with a membrane-bound antigen which occurs on all T cells. MT1 also reacts with thymocytes, monocytes, macrophages, epidermal Langerhans cells and the Kupffer cells of the liver, as well as with myeloid cells and erythrocyte precursors. The MT1 antigens are not found on mature or activated B cells. This mixture of monoclonal antibodies MT1 and MB1 stains membrane of all leukocytes, and recognizes all T and B cells, as well as NK cells, myeloid cells, monocytes, histiocytes and erythrocyte precursors. CD43 (T Cell, Leukosialin) CD44 (phagocytic glycoprotein-1, homing cell adhesion molecule, HCAM, CD44s) is a cell surface 80-90 kD glycoprotein important in lymphocyte homing, T-cell activation and adhesion to hyaluronate and matrix proteins. It is expressed on the surface of a wide variety of cells, among which are T-cells, B-cells, monocytes, fibroblasts, keratinocytes, vascular endothelial cells, columnar epithelium of the GI tract, and transitional epithelium of the urinary tract. This antibody stains CD44 antigen in cell membranes of various cells such as T cells, B cells, monocytes, granulocytes and even on most erythrocytes, epithelial cells, central nervous white matter, fibroblasts, skeletal muscle and on a wide variety of tumors. P Clone: DFT-1 Isotype: IgG1 Kappa Source: Mouse Immunogen: Immature pluripotential human leukemia cell line K562 Specificity: Tonsil stained with Anti-CD43 using DAB chromogen P CD43 and lymphoma or leukemia subtyping Localization: Membrane Pre-treatment: AR Citra/EZ-AR 1 Manual: HK087-5KE Xmatrx: HX031-YCDE Ready-to-use (Manual): AM305-5M Ready-to-use (Automated): Xmatrx® AX305-YCD Concentrated: MU305-UC Recommended Positive Control: FG-305M The CD43 antigen, also known as leukosialin or sialophorin is a 95110 kD protein. Monoclonal DFT-1 reacts with this protein on T cells and thymocytes and a 115-135 kD molecule on neutrophils and platelets. In addition, the CD43 epitope is present on many cells such as granulocytes, monocytes, macrophages, NK cells, platelets, activated B cells, plasma cells, epidermal Langerhans cells and also on bone marrow hematopoietic stem cells. This antibody stains CD43, a membrane-bound antigen found on all T cells, macrophages, monocytes, and epidermal Langerhans cells. 72 CD45 (Leukocyte Common Antigen, LCA) P - Tissue Type FFPE F - Tissue Type Frozen Clone: PD7/26/16 & 2B11 Isotype: IgG1 Kappa Source: Mouse Immunogen: Human lymphocytes Specificity: CD45 Localization: Membrane Pre-treatment: None Tonsil expressing strong LCA positivity using DAB chromogen Ready-to-use (Manual): AM111-5M Ready-to-use (Automated): i6000TM Xmatrx® AM111-10M AX111-YCD Recommended Positive Control: FG-111M This antibody against CD45 (Leukocyte Common Antigen) recognizes the 200 kD antigen found on lymphoid cells, macrophages, histiocytes, and neutrophils. CD45 is helpful in determining the leukocytic nature of anaplastic tumors. Combined with other antibodies such as those to cytokeratins and S-100 protein, this monoclonal antibody to leukocyte common antigen can be used in the characterization of undifferentiated large cell neoplasms. Most neoplastic B cells and T cells stain positive in leukemia and in non-Hodgkins lymphomas, whereas most neoplastic myeloid and erythroid cells are negative. This antibody labels lymphoid cells and to a lesser extent macrophages, histiocytes, and granulocytes. www.biogenex.com Primary Antibodies CD45 (Leukocyte Common Antigen, LCA) CD45RA (B Cell) P Spleen Tissue stained with AntiCD45 using DAB chromogen P Clone: LJ 27.9 Clone: MB1 Isotype: IgG1 Isotype: IgG1 Source: Mouse Source: Mouse Immunogen: Human lymphocytes Immunogen: Specificity: Leukocyte Common Antigen Hodgkin’s lymphoma cell line DEV Specificity: CD45RA Localization: Membrane Localization: Membrane Pre-treatment: EZ-AR 1/EZ-AR 2 Pre-treatment: None Manual: None Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): Tonsil stained with Anti-CD45R using DAB chromogen AM338-5M Ready-to-use (Automated): Xmatrx® Concentrated: AX338-YCD MU338-UC The Leukocyte Common Antigen consists of a family of heavily glycosylated glycoproteins of apparent MW 180-240kD. CD45 may function in the regulation of L-selectin (CD62L), in regulation of B-lymphocyte negative and positive selection and in T-cell activation. It stains lymphocytes, monocytes, eosinophils, and also neoplastic cells of lymphoid origin. Neoplastic B cells and T cells in leukemia and in non-Hodgkin's lymphomas stain positive. This antibody stains CD45 antigen in membrane and cytoplasm of the majority of human leukocytes. CD45 Cocktail (Leukocyte Common Antigen, LCA) Ready-to-use (Manual): AM157-5M Ready-to-use (Automated): i6000TM Xmatrx® AM157-10M AX157-YCD Recommended Positive Control: FG-157M Recommended Barrier Control: FB-157M CD45RA is a restricted isoform of LCA of about 220 kD. MB1 recognizes a membrane-bound antigen which occurs on all B cells with the exception of plasma cells, and on some mature T cells. The antigen which is identified by this monoclonal antibody is not present on immature T cells. This antibody stains CD45RA antigen on the membrane of all B cells with the exception of plasma cells and some mature T cells. P Clone: MEM55+LJ 27.9 Isotype: IgG1 Source: Mouse Clone: MEM55 Immunogen: Human lymphocytes Isotype: IgG1 Specificity: CD45 Source: Mouse Localization: Membrane Immunogen: Human lymphocytes Pre-treatment: None Specificity: CD45RB antigen CD45RB P LCA positivity on Anaplastic Large Cell Lymphoma stained using DAB chromogen Tonsil stained with Anti-CD45RB using DAB chromogen Localization: Membrane Pre-treatment: EZ-AR 1/EZ-AR 2 Manual None Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): AM371-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM371-10M AX371-YCD Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® AX320-YCD MU371-UC Concentrated: MU320-UC Recommended Positive Control: Recommended Barrier Control: FG-371M FB-371M Recommended Positive Control: FG-320M CD45 (LCA) is a transmembrane protein-tyrosine-phosphatase. The LCA family includes a group of proteins present on all mature B and T lymphocytes, thymocytes, macrophages, spleen, lymph node, chronic lymphatic leukemia cells, bone marrow, thymus, and granulocytes. It is absent in brain, kidney, liver, heart, erythrocytes, platelets, and normal serum. This antibody may be useful in the evaluation of malignant lymphoma and nonlymphoid tumors. Neoplastic B and T cells in leukemia and in non-Hodgkin's lymphoma stain positive and hence can be distinguished from sarcomas and carcinomas. This antibody stains CD45 antigen on the membrane of most leukocytes. internationalcs@biogenex.com AM320-5M CD45RB, which includes three glycoproteins with molecular mass of 190, 205 and 220 kD, has been found to belong to the CD45 family. Monoclonal antibody MEM55 can be used to stain CD45RB antigen on most T cells, B cells, monocytes, and macrophages. The cellular distribution of CD45RB is very similar to that of other conventional CD45 antibodies, except that some cells, such as Langerhan's cells and a small subset of T cells, are negative. This antibody stains the CD45RB antigen, the isoform of the leukocyte common antigen encoded by exon B. P - Tissue Type FFPE F - Tissue Type Frozen 73 Primary Antibodies CD45RC (T Cell) CD48 P P Clone: MT2 Clone: EP148 Isotype: IgG1 Isotype: IgG Source: Mouse Source: Rabbit Immunogen: Lymph node involving chronic lymphatic leukemia Immunogen: A synthetic peptide corresponding to residues of human CD48 protein CD48 protein Specificity: CD45RC Localization: Membrane Specificity: Pre-treatment: None Localization: Membrane Pre-treatment: EZ-AR™ 2 Manual/i6000: HK522-XAK Xmatrx: None Tonsil stained with anti-CD48 using DAB chromogen T Cells in Tonsil stained with AntiCD45RC using DAB chromogen Ready-to-use (Manual): AM156-5M Recommended Positive Control: MU156-UC FG-156M Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Recommended Barrier Control: FB-156M Recommended Positive Control: AN721-10ME AY721-YCDE NU721-UCE FG-721N Recommended Barrier Control: FB-721N Ready-to-use (Automated): Xmatrx® AX156-YCD Concentrated: Clone MT2 has previously been described as CD45RA but due to its reactivity with transfectants and its identical staining pattern with ORTH75E4 it is now recognized as CD45RC.FB-156M Clone MT2 reacts with membrane-bound antigen which is present on mature, non-activated T and B cells. It reacts with medullary thymocytes, with mantle zone lymphocytes in follicles of lymph nodes and spleen, with lymphocytes of the paracortex, with peripheral blood B cells, with T suppressor/ cytotoxic cells and NK cells. This clone is used for differentiation of non-Hodgkin lymphomas. CD45RO (T Cell) CD48 (BLAST1) is an activation-associated, glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein expressed primarily in mitogen-stimulated human lymphocytes. CD48 is expressed on T cells, B cells, thymocytes and splenocytes. Both normal and malignant white blood cells express CD48 on their membrane surface, but greater than 95% of CD34+ hematopoietic stem cells do not express CD48. CD48 is expressed at higher levels on human Burkitt’s lymphoma cell lines, Raji and most acute myeloid leukemia cells with phenotype CD34-/ CD13+/CD33+. P Clone: UCHL-1 Isotype: IgG 2a Kappa Source: Mouse Immunogen: IL-2 dependent T cell line CA1 Specificity: T cells Localization: Membrane Pre-treatment: None Tonsil stained with Anti-CD45R0 using DAB chromogen CD53 P Tonsil stained with anti-CD53 using DAB chromogen Ready-to-use (Manual): AM113-5M Ready-to-use (Automated): i6000TM Xmatrx® AM113-10M AX113-YCD Concentrated: MU113-UC Recommended Positive Control: FG-113M Recommended Barrier Control: FB-113M This antibody recognizes a 185 kD molecule (CD45RO) which occurs on mature activated T cells, most thymocytes, and a sub-population of resting T cells within both the CD4 and CD8 subsets. UCHL-1 shows no reactivity with normal B or NK cells, but will react with granulocytes and monocytes. This antibody can be used as a marker of T cell lymphomas and other T cell neoplasms. The antigen has been shown to be immunologically unrelated to the lymphocyte-function-associated antigen (LFA-1), which has a similar molecular weight. This antibody stains the membrane and sometimes the cytoplasm of CD45RO positive cells. 74 AN721-5ME P - Tissue Type FFPE F - Tissue Type Frozen Clone: EP179 Isotype: IgG Source: Rabbit Immunogen: A synthetic peptide corresponding to residues of human CD53 Specificity: CD53 Localization: Membrane Pre-treatment: AR Citra Plus Manual/i6000: HK081-5KE Xmatrx: None Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN734-5M Recommended Positive Control: AN734-10M AY734-YCD NU734-UC FG-734N Recommended Barrier Control: FB-734N Leukocyte surface antigen CD53 is a protein that in humans is encoded by the CD53 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. The function of this family in a number of different cell types may be involved in transmembrane signal transduction and regulation of cell proliferation and differentiation, or both. CD53 is broadly expressed on leukocytes, including B cells, T cells, monocytes and granulocytes. It has been demonstrated to be a specific and reliable marker for leukocytes. This antibody strongly labels normal and neoplastic cells with hematopoietic origin. www.biogenex.com Primary Antibodies CD56 (Natural Killer Cell, NCAM) CD61 F Clone: EP2417Y Isotype: IgG1 Kappa Isotype: IgG Source: Mouse Source: Rabbit Immunogen: A synthetic peptide corresponding to residues near the C-terminus (the cytoplasmic region) of human Integrin ß-3A. Specificity: CD 61 Immunogen: Frozen Tonsil stained with Anti-CD56 using AEC chromogen P NKH-1 Clone: Human chronic myeloid leukemia cells Specificity: CD56 (NKH-1) Localization: Membrane Pre-treatment: EZ-AR 1/EZ-AR 2 Manual: None Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): Kidney tissue stained with AntiIntegrin ß-3A (CD61) using AEC chromogen AM268-5M Localization: Membrane Pre-treatment: EZ-AR™ 2/EZ-AR 1 Manual/i6000: HK522-XAK Xmatrx: HX031-YCDE Ready-to-use (Automated): Xmatrx® AX268-YCD Ready-to-use (Manual): AN482-5M Concentrated: MU268-UC Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN482-10M AX482-YCD Recommended Positive Control: FG-482N The NKH-1 antigen is present on a subpopulation of 10 to 15% of human peripheral blood lymphocytes (PBL) and is expressed on all cells which mediate non-MHC restricted cytotoxicity. Therefore, it is a pan natural killer (NK) cell antigen. This antibody reacts with one of the three distinct epitopes that have been identified: the NKH1b epitope. It can also be of value in the immunophenotyping of tumors derived from neuroectodermal tissue. This antibody stains CD56 (NKH-1) on peripheral blood large granular lymphocytes in frozen tissue sections. CD57 (Natural Killer Cell) P NU482-UC Platelet membrane glycoprotein Integrin ß-3 (GP IIIa) forms a Ca2+dependent heterodimer complex with GP Ilb. The GP Ilb-IIIa complex constitutes the fibrinogen and fibronectin receptor on stimulated platelets. A biochemically and immunologically similar member glycoprotein complex is present on endothelial cells. Data suggest that tyrosine phosphorylation of an integrin beta subunit may be important in initiating outside-in signaling cascades by inducing association of signaling components directly with the integrin. Clone: NK-1 Isotype: IgM Source: Mouse Specificity: CD57 (natural killer cell, also called HNK1) Clone: EP211 Localization: Membrane & Cytoplasm Isotype: IgG Pre-treatment: None Source: Rabbit Immunogen: A synthetic peptide corresponding to residues of human CD63 Specificity: CD63 CD63 P Tonsil stained with Anti-CD57 using DAB chromogen Ready-to-use (Manual): AM314-5M Ready-to-use (Automated): Xmatrx® AX314-YCD Concentrated: MU314-UC Recommended Positive Control: FG-314M Prostate tissue stained with antiCD63 using DAB chromogen Monoclonal antibody NK-1recognizes CD57, also called HNK-1 or Leu 7 antigen. It is a 110 kD myeloid, cell-associated surface glycoprotein. The antigen is common to leukocytes and neuroectodermal cells. It is present in most cancers with neuronal as well as glial characteristics. Tumors and normal cells derived from the neuroectoderm or the APUD (diffuse neuroendocrine system) tumors also express this antigen. Anti-natural killer cell antibodies used in combination with anti-S-100 antibodies aid in the differentiation of Schwann cell neoplasms from histologically similar fibrosarcomas. This antibody stains CD57 on the membrane of natural killer cells in both normal and abnormal tissues. internationalcs@biogenex.com Localization: Membrane/Cytoplasm Pre-treatment: EZ-AR™ 2/ EZ-AR 1 Manual/i6000: HK522-XAK Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN720-5ME Recommended Positive Control: AN720-10ME AY720-YCDE NU720-UCE FG-720N Recommended Barrier Control: FB-720N CD63 is a 53 kD lysosomal membrane glycoprotein is expressed on activated platelets, monocytes and macrophages, also weakly expressed on granulocytes, T cell and B cells. It is strongly expressed in early melanoma, breast carcinoma, merkel cell carcinoma, astrocytoma and lung adenocarcinoma. Recent reports also indicate that CD63 is a good prognostic biomarker for human astrocytomas and earlier stages of lung carcinoma. P - Tissue Type FFPE F - Tissue Type Frozen 75 Primary Antibodies CD66 CD68 P BY114 Clone: CD68/G2 Isotype: IgG Isotype: IgG1 Source: Mouse Source: Mouse Immunogen: Human B cell lymphoma Immunogen: Human CD68 Specificity: CD66 antigen Specificity: CD68 Localization: Membrane Localization: Cytoplasm Pre-treatment: None Pre-treatment: EZ-AR™ 2/EZ-AR 1 Manual/i6000: HK522-XAK Xmatrx: HX031-YCDE Histocytoma stained with Anti-CD68 using DAB chromogen Reactive node stained with AntiCD66 using DAB chromogen Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Recommended Positive Control: AM325-5M Ready-to-use (Manual): AM549-5M AX325-YCD FG-325M Ready-to-use (Automated): i6000TM Xmatrx® AM549-10M AX549-YCD Concentrated: MU549-UC Recommended Positive Control: FG-549M Clone BY114, also known as NCA90 (Non-cross reacting antigen 90), is a unique monoclonal antibody that recognizes CD66CE which is a 90 kD antigen found principally on neutrophils. In contrast to many antibodies which recognize granulocyte-associated antigens present on other leukocytes, this antibody recognizes only granulocytes. The anti-CD66 monoclonal antibody, therefore, is very useful for differentiation of normal and neoplastic cells of granulocyte origin. Monoclonal antibody BY114 can be used to stain neutrophils in tonsil, spleen, liver, kidney, pancreas, and lung. This antibody stains phosphatidylinositol (PI) linked protein on granulocyte and squamous epithelium. CD68 P Lymph node stained with Anti-CD68 using DAB chromogen The CD68 antigen is a heavily glycosylated transmembrane protein of 87-115 kD which is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. This antibody is capable of staining monocytes, Kupffer cells, osteoclasts, granulocytes and their precursors; Lymphomas are negative or show a few granules. This antibody may be useful for the identification of myelomonocytic and histiocytic tumors. CD68 may help to distinguish malignant fibrous histiocytoma from other pleomorphic sarcomas. However, since CD68 detects a formalin-resistant epitope that may be associated with lysosomal granules, other lysosome-rich cells may also produce positive results. Clone: KP1 Isotype: IgG1 Kappa Source: Mouse Immunogen: Lysosomal granules from human lung macrophage Clone: T9 Isotype: IgM Kappa Specificity: Macrophages Source: Mouse Localization: Cytoplasm Immunogen: Pre-treatment: AR Citra Plus/EZ-AR 1 Manual/i6000 HK081-5KE Human T cell chronic lymphocytic leukemia cells Xmatrx: HX031-YCDE Specificity: CD71 (Transferrin Receptor T9) Ready-to-use (Manual): AM416-5M Ready-to-use (Automated): i6000TM Xmatrx® AM416-10M AX416-YCD Concentrated: MU416-UC Recommended Positive Control: FG-416M CD68 antigen is a 110-kD type 1 membrane glycoprotein, appears in endosomes or lysosomes (long variant) and to a lesser extent on the cell surface (short variant). It is highly expressed by blood monocytes and tissue macrophages. It is also reported to be expressed in immature myeloid cells, lymphoma, many tumor cell lines, and some epithelial tumors, although the labeling is usually less intense than in macrophages. Clone KP1 reacts strongly with a fixative-resistant epitope of CD68 protein that is expressed by virtually all macrophages of the human body. The CD68 antibody can be used as part of a panel in the evaluation of poorly differentiated neoplasms in cytological materials. 76 P Clone: P - Tissue Type FFPE F - Tissue Type Frozen CD71 (Transferrin Receptor) F Frozen Liver stained with Anti-CD71 using DAB chromogen Localization: Membrane Pre-treatment: EZ-AR 1/EZ-AR 2 Manual: None Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): AM269-5M Ready-to-use (Automated): Xmatrx® AX269-YCD This antibody reacts with CD71 antigen (also known as T9 or Transferrin Receptor), a homodimeric type II membrane protein consisting of two identical subunits of approximately 95 kD covalently linked by two intermolecular disulfide bonds. This antigen has also been identified on the endothelium of brain capillaries, on carcinomas and sarcomas of various origins as well as on both high- and low-grade malignant lymphomas. This antibody stains the T9 antigen activated lymphocytes, myelocytes, and nucleated erythrocyte precursors in frozen tissue sections. www.biogenex.com Primary Antibodies CD79a CD71 (Transferrin Receptor) P Clone: Clone: 11E3 IgG2a IgG1 Isotype: Source: Mouse Source: Mouse Immunogen: Baculovirus-expressed, recombinant human Transferrin Receptor Immunogen: Recombinant CD79a protein internal domain Specificity: CD79a antigen Specificity: CD71 (Transferrin Receptor) Localization: Membrane Pre-treatment: AR Citra Plus/EZ-AR 2 Manual/i6000: HK081-5KE Xmatrx: HX032-YCDE Isotype: Tonsil stained with Anti-CD71 using DAB chromogen Localization: Membrane & Cytoplasm Pre-treatment: AR Citra/EZ-AR 1/EZ-AR 2 Manual: HK087-5KE Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Concentrated: AM354-5M AX354-YCD MU354-UC This antibody reacts with CD71 antigen (also known as T9 or Transferrin Receptor), a homodimeric type II membrane protein consisting of two identical subunits of approximately 95 kD covalently linked by two intermolecular disulfide bonds. Transferrin Receptor is present on 10% of thymocytes, activated lymphocytes, myelocytes, and nucleated erythrocyte precursors. Broad distribution of Transferrin Receptor (TR) has been observed on carcinomas and sarcomas of various origins and malignant lymphomas. Clone H68.4 is specifically directed against the human Transferrin Receptor cytoplasmic tail. This antibody stains the cytoplasm and membrane of activated lymphocytes and erythroid precursors. CD74 (B Cell) P H68.4 Tonsil tissue stained with AntiCD79a using DAB chromogen Ready-to-use (Manual): AM414-5M Ready-to-use (Automated): i6000TM Xmatrx® AM414-10M AY414-YCD Concentrated: MU414-UC Recommended Positive Control: FG-414M CD79a, also known as Ig-alpha, mb-1 membrane glycoprotein, is a type I membrane glycoprotein with a total of 226 amino acids and a molecular weight of 47 kD. CD79a forms a heterodimer with CD79b through disulfide-bonds and further forms a complex in a noncovalent fashion with membrane immunoglobulins. Both CD79a and CD79b are expressed almost exclusively on B cells and B-cell neoplasms. In addition, CD79a and CD79b antibodies are useful markers in the evaluation of precursor B-acute lymphoblastic leukemia (pre-B-ALL) because many of these tumors are negative for other B-cell markers, such as CD20 and CD45RA. P Clone: LN2 Isotype: IgG1 Source: Mouse Immunogen: Nuclei from diffuse histiocytic lymphoma cells (SU-DHL-4) Specificity: Tonsil stained with Anti-CD74 using DAB chromogen CD79a P Clone: EP82 Isotype: IgG Source: Rabbit Immunogen: A synthetic peptide derived from the extracellular region of human CD79a protein CD79a CD74 Localization: Membrane & Cytoplasm Pre-treatment: EZ-AR 1/EZ-AR 2 Specificity: Xmatrx: HX031-YCDE/HX032-YCDE Localization: Membrane/Cytoplasm Manual: None Pre-treatment: AR Citra Plus/ EZ-AR 1 Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE Ready-to-use (Manual): Lymph node stained with antiCD79a using DAB chromogen AM153-5M Ready-to-use (Automated): Xmatrx® Concentrated: MU153-UC Recommended Positive Control: FG-153M AX153-YCD LN2 recognizes the 35 kD Class II invariant chain expressed in the nuclear membrane and cytoplasm of B lymphocytes and is suitable for differentiating between B-cell and T-cell lymphomas. It reacts with a nuclear membrane antigen expressed by B cells of mantle zones and germinal centers, and with the nuclear membrane of interdigitating cells in lymph nodes. It also reacts with Reed-Sternberg cells and their variants in Hodgkin’s disease, and sporadically with antigens expressed by tumor cells of epithelial origin. This antibody stains nucleus, membrane and cytoplasm of B-cells. internationalcs@biogenex.com Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN719-5M Recommended Positive Control: AN719-10M AY719-YCD NU719-UC FG-719N Recommended Barrier Control: FB-719N CD79 consist of two proteins, CD79a (mb-1) and CD79b (B29). CD79a recognizes the Ig-alpha protein, and CD79b recognizes the Ig-beta protein of the B-cell antigen component of the B-lymphocyte antigen receptor. CD79a is an excellent marker for identification of normal and neoplastic B lymphocytes. It has been found to be co-expressed with CD3 in 10% of cases of T-lymphoblastic leukemia/lymphoma. Antibodies to CD79α may also be useful in the differential diagnosis of Hodgkin’s disease. P - Tissue Type FFPE F - Tissue Type Frozen 77 Primary Antibodies CD90 CD99 P Thymus stained with anti-CD90 EP56 Clone: EPR3097Y Isotype: IgG Isotype: IgG Source: Rabbit Source: Rabbit Immunogen: A synthetic peptide corresponding to residues near the N-terminus of human CD90 protein Immunogen: A synthetic peptide corresponding to residues on the Cterminus of human CD99 Specificity: Human CD90 protein Specificity: CD99 Localization: Membrane/Cytoplasm Pre-treatment: AR-10 Manual/i6000: HK058-5K Xmatrx: None Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN733-5M Recommended Positive Control: AN733-10M AY733-YCD NU733-UC FG-733N Recommended Barrier Control: FB-733N CD90 is expressed on thymocytes, neurons, glial cells, endothelial cells, fibroblasts, fetal liver cells and hematopoietic stem cells in normal bone marrow and cord blood. Thy-1 has been used as a marker for a variety of stem cells and for the axonal processes of mature neurons. CD90 is associated with unfavorable clinical and biological features in acute myeloid leukemia. In prostate cancer, CD90 has been reported to be overexpressed in cancer associated fibroblasts and serves as a marker for prostate cancer-associated stroma. CD95 P Tonsil stained with anti-CD95 using DAB chromogen Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Ewing’s Sarcoma stained with AntiCD99 using DAB chromogen Localization: Membrane Pre-treatment: EZ-AR™ 1/EZ-AR 2 Manual/i6000: HK521-XAK Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): AN518-5M Ready-to-use (Automated): i6000TM Xmatrx® AN518-10M AX518-YCD Concentrated: NU518-UC Recommended Positive Control: FG-518N CD99, or Protein MIC2, is a T-cell surface glycoprotein involved in the T-cell adhesion process and spontaneous rosette formation with erythrocytes. The cell surface antigen defined by the monoclonal antibody CD99 is expressed by both the human X and Y chromosomes; the gene loci on the X and Y chromosomes are referred to as MIC2X and MIC2Y, respectively. It was demonstrated that engagement of distinct epitopes on CD99 rapidly induces T cell death by a novel caspase-independent pathway. Thus, it is suggested that CD99 is linked to a novel death pathway that may have biologic relevance in control of early T cells. CD99 (MIC2 Antigen) Clone: EP208 Isotype: IgG Clone: HO36.1.1 Source: Rabbit Isotype: IgM Immunogen: A synthetic peptide corresponding to residues of human CD95 protein Source: Mouse Immunogen: Purified E-rosette forming cells from human peripheral blood lymphocytes CD99 (MIC2 antigen) P Specificity: CD95 Localization: Membrane/Cytoplasm Specificity: Pre-treatment: EZ-AR™ 2 Localization: Membrane Manual/i6000: HK522-XAK Pre-treatment: AR-10/EZ-AR 2 Xmatrx: None Ewings sarcoma stained with Anti-CD99 stained using DAB chromogen Manual: HK058-5KE Xmatrx: HX032-YCDE AN742-5M AN742-10M AY742-YCD NU742-UC The CD95 (Fas) protein is a cell surface receptor belonging to the tumor necrosis factor (TNF) family that transduces death signaling on engagement by multimeric Fas ligand (CD95L). CD95 plays a major role in determining morphological and functional maturity in a variety of systems, including the formation of the neural network and clonal deletion of autoreactive T cells. The expression of CD95 serves as a prognostic marker in predicting the outcome of disease progression and treatment in many types of tumors. 78 P Clone: P - Tissue Type FFPE F - Tissue Type Frozen Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM355-5M AM355-10M AX355-YCD MU355-UC CD99 is a 32 kD membrane glycoprotein expressed by human thymocytes, most T-ALL cells, some red blood cells, and the small cell round tumors of Ewing’s sarcoma and peripheral neuroectodermal tumors. The CD99 protein is known to be involved in T-cell-adhesion events. CD99 has been found to be expressed in lymphoblastic lymphomas, large cell lymphomas, and many cases of pediatric acute lymphocytic leukemia. This antibody stains CD99 antigen in human thymocytes and some T-ALL isolates and other positive cells. www.biogenex.com Primary Antibodies CD103 CD117 P Colon carcinoma stained with antiCD103 using DAB chromogen P Clone: EP206 Clone: T595 Isotype: IgG Isotype: IgG1 Kappa Source: Rabbit Source: Mouse Immunogen: A synthetic peptide corresponding to residues of human CD103 protein Immunogen: Recombinant protein corresponding to the three N-terminal C2-like extracellular domains. Specificity: c-Kit protein (CD117) Specificity: CD103 Localization: Membrane Pre-treatment: EZ-AR™ 2 Manual/i6000: HK522-XAK Xmatrx: None Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Appendix tissue stained with AntiCD117 using DAB chromogen Recommended Positive Control: Recommended Barrier Control: FB-739N CD103, also known as integrin alpha E (ITGAE), is an integrin protein that in humans is encoded by the ITGAE gene. CD103 is expressed on intraepithelial lymphocytes in mucosal areas, including lung and GI tract. In malignancies, CD103 is expressed on more than 95% of intraepithelial CD8+ cells and on 40% of mucosa-associated T cells, whereas less than 2% of resting blood lymphocytes are CD103-positive. In several malignant conditions, such as T-cell lymphomas and hairy cell leukemia, the cells express CD103. AR Citra Plus/EZ-AR 1 Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE Ready-to-use (Manual): AM423-5M Ready-to-use (Automated): i6000TM Xmatrx® AM423-10M AX423-YCD Concentrated: MU423-UC Recommended Positive Control: FG-423M c-Kit (CD117) is a transmembrane, tyrosine kinase receptor and protooncogene product which is expressed on numerous diverse fetal and adult cells including hematopoietic cells, mast cells, melanocytes, germ cells, and the interstitial cells of Cajal. Its expression in tumors is also diverse. CD117 P CD105 Clone: 4G11 Clone: YR145 Isotype: IgG Source: Rabbit IgG2a Immunogen: CD117 antigen Source: Mouse Specificity: CD117 Immunogen: Prokaryotic recombinant protein corresponding to a region of the external domain of the CD105 glycoprotein. Localization: Membrane and Cytoplasm Pre-treatment: EZ-ARTM 1/EZ-AR 2 Manual/i6000: HK521-XAK Xmatrx: HX031-YCDE/HX032-YCDE Isotype: Uterine blood vessels stained with Anti-CD105 using DAB chromogen Membrane & Cytoplasm Pre-treatment: AN739-5ME AN739-10ME AY739-YCDE NU739-UCE FG-739N P Localization: Specificity: CD105 Localization: Membrane Pre-treatment: AR-10/EZ-AR 1/EZ-AR 2 Manual: HK058-5KE Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): AM441-5M Ready-to-use (Automated): Xmatrx® AX441-YCD Concentrated: MU441-UC Recommended Positive Control: FG-441M CD105 (endoglin) is a proliferation-associated and hypoxia-inducible protein abundantly expressed in angiogenic endothelial cells. It is a receptor for Transforming Growth Factor (TGF) -beta1 and -beta3 and modulates TGF-beta signaling by interacting with TGF-beta receptors I and/or II and hence driving tumor growth and metastasis. Endoglin (CD105) is a better marker to identify proliferating endothelium involved in tumor angiogenesis than pan-endothelial markers such as CD31, CD34 and Factor VIII etc. It can be used as a marker for microvessel density measurement and also in tumor imaging. This antibody stains membrane and cytoplasm of activated endothelial cells. internationalcs@biogenex.com GIST stained with Anti-CD117 using DAB chromogen Ready-to-use (Manual): AN465-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN465-10M AX465-YCD NU465-UC Recommended Positive Control: FG-465N CD117 / c-Kit is a transmembrane tyrosine kinase encoded by the c-Kit proto oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Mutations in c-Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer. Phosphorylation at tyrosine 721 of c-Kit allows binding and activation of PI3 kinase. P - Tissue Type FFPE F - Tissue Type Frozen 79 Primary Antibodies CD146 CD227 (Mucin 1) P Placenta stained with anti-CD146 using DAB chromogen EP54 Clone: VU-4H5 Isotype: IgG Isotype: IgG1 Source: Rabbit Source: Mouse Immunogen: A synthetic peptide corresponding to residues in human CD146 protein Immunogen: 60mer tandem repeat of VTSAPDTRPAPGSTA -PPAHG,conjugated to BSA Specificity: CD146 protein Specificity: CD227 (MUCIN 1) Localization: Membrane/Cytoplasm Localization: Cytoplasm Pre-treatment: AR Citra Plus/EZ-AR 2 Pre-treatment: EZ-AR™ 2 /EZ-AR 1 Manual/i6000: HK081-5KE Manual/i6000 HK522-XAK Xmatrx: HX032-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN716-5M Recommended Positive Control: AN716-10M AY716-YCD NU716-UC FG-716N Recommended Barrier Control: FB-716N CD146 (cluster of differentiation 146) labels endothelial cells, smooth muscle cells, intermediate trophoblast, subpopulation of T cells, and peripheral neuronal cells. In tumor, CD146 is expressed on tumor cells derived from peripheral nerves system, melanoma and clear cell sarcoma. CD146 has been used as a marker for intermediate trophoblast. It has been reported that CD146 is useful in differentiation of mesothelioma (CD146 positive) and reactive mesothelium (CD146 negative). CD146 is associated with tumor progression and the development of metastasis in human malignant melanoma. CD205 Mucinous adenocarcinoma stained with Anti-CD227 using DAB chromogen Xmatrx: Clone: EP176 Isotype: IgG Source: Rabbit Immunogen: A synthetic peptide corresponding to residues of human CD205 protein Specificity: CD205 Localization: Membrane/Cytoplasm Pre-treatment: AR Citra Plus/EZ-AR 1 Manual/i6000: HK81-5KE Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Recommended Positive Control: Recommended Barrier Control: AN737-5ME AN737-10ME AY737-YCDE NU737-UCE FG-737N FB-737N CD205 is predominantly expressed by the thymic cortical epithelium and by dendritic cells (DC), but can also be detected at low levels in T and B lymphocytes and several other epithelial cell types. CD205 is a novel thymic epithelial marker that is important for the positive selection process of thymocytes. It is a sensitive and specific marker for thymoma, while the sensitivity to thymic carcinoma is lower than CD5 and CD117. P - Tissue Type FFPE F - Tissue Type Frozen HX031-YCDE Ready-to-use (Manual): AM534-5M Ready-to-use (Automated): i6000TM Xmatrx® AM534-10M AX534-YCD Concentrated: MU534-UC Recommended Positive Control: FG-534M Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227, also known as mucin 1 is a breast cancer associated mucin encoded by the Muc-1 gene. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is over expressed abundantly in >90% breast carcinomas and metastases. CDw75 (B Cell) P P Tonsil stained with anti-CD205 using DAB chromogen 80 P Clone: Tonsil stained with Anti-CDw75 using AEC chromogen Clone: LN1 Isotype: IgM Source: Mouse Immunogen: Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes Specificity: CDw75 antigen Localization: Membrane & Cytoplasm Pre-treatment: EZ-AR 1/EZ-AR 2 Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Concentrated: AM152-5M AX152-YCD Recommended Positive Control: FG-152M MU152-UC CDw75 is proposed as the ligand for CD22 mediating B-cell to B-cell interaction. Clone LN1 recognizes a sialoantigen on cell membranes. On tissue sections, it causes a marked reaction with the B lymphocytes in germinal centers, but only a faint reaction with B lymphocytes of the mantle zone in lymphatic tissues. LN1 also reacts with various types of epithelial cells, including cells of the distal renal tubules, breast, bronchus, prostate, and erythrocytes. This antibody stains CDw75 antigen on cell membranes of B lymphocytes in the germinal centers, certain epithelial cells, including cells of the distal renal tubules, breast, bronchus and prostate. www.biogenex.com Primary Antibodies CDX-2 c-erbB-2 (HER-2/neu) P Intestine tissue stained with AntiCDX2 using DAB chromogen P Clone: CDX2-88 Clone: EP3 Isotype: IgG 1 Kappa Isotype: IgG Source: Mouse Source: Rabbit Immunogen: A Balb/c mouse was immunized with a full-length CDX2 recombinant protein. Stable hybridomas were produced by fusion of spleen cells with P2/0 myeloma cell. Immunogen: A synthetic peptide corresponding to residues surrounding tyrosine 877 of human HER2 Specificity: Her2 Breast stained with anti-Her2 using DAB chromogen Membrane Pre-treatment: AR Citra Plus Specificity: CDX2 protein Localization: Nucleus Manual/i6000: HK081-5KE Pre-treatment: AR Citra Plus/EZ-AR 2 Xmatrx: None Manual/i6000: HK081-5KE Xmatrx: HX032-YCDE Ready-to-use (Manual): AM392-5M Ready-to-use (Automated): i6000TM Xmatrx® AM392-10M AX392-YCD Concentrated: MU392A-UC Recommended Positive Control: FG-392M Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Recommended Positive Control: Recommended Barrier Control: CDX2, a member of the caudal-related homeobox family, is an intestine-specific transcription factor that regulates both proliferation and differentiation in intestinal epithelial cells. It plays an important role in triggering cells towards the phenotype of differentiated villus enterocytes as well as in the maintenance of the phenotype. Clone CDX2-88 reacts with a conserved epitope of the 40kD CDX2 protein localized in the nucleus. It exclusively marks nuclei of colonic epithelial cells and colorectal cancers on formalin-fixed, paraffin-embedded tissue sections. c-erbB-3 (HER-3) CB11 Isotype: IgG1 Source: Mouse Immunogen: Synthetic peptide corresponding to a site on the internal domain of the c-erbB-2 Protein (HER-2/neu) Specificity: Her 2 Localization: Membrane Pre-treatment: None Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Recommended Positive Control: Recommended Barrier Control: Breast carcinoma stained with Antic-erbB-3 using DAB chromogen AM134-5ME AM134-10ME AX134-YCDE MU134-UCE FG-134M FB-134M Her-2/neu (c-erb-B2) gene product is a 185 kD transmembrane glycoprotein associated with tyrosine kinase activity. The antibody CB11 is directed against the internal domain of this oncoprotein. Approximately 20-30% cases of breast cancer show an amplification and/or overexpression of Her-2 in tumor cells. Since there is an availability of the target therapy to Her-2 Neu positive tumors, the lab testing of Her-2/neu in breast carcinoma becomes very important in patient care. internationalcs@biogenex.com AN726-10ME AY726-YCDE NU726-UCE FG-726N FB-726N RU O P P Clone: AN726-5ME HER2 (human epidermal growth factor receptor 2), also known as Neu, ErbB-2, CD340 (Cluster of differentiation 340) or p185, is a protein that is encoded by the ERBB2 gene. HER2 is a member of the epidermal growth factor receptor (EGFR/ErbB) family. The kinase activity of the ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins. Overexpression of ErbB2 is detected in almost 40% of human breast cancers. For research use only. Not for use in diagnostic procedures. c-erbB-2 (HER-2/neu) Breast carcinoma stained with AntiHer2 using DAB Chromogen Localization: Clone: RTJ1/A2 Isotype: IgM Source: Mouse Immunogen: Synthetic peptide from the cytoplasmic domain of the human c-erbB-3 protein Specificity: c-erbB-3 protein Localization: Membrane Pre-treatment: EZ-AR 1/EZ-AR 2 Manual: HK167-5K Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): AM319-5M Ready-to-use (Automated): Xmatrx® Concentrated: AX319-YCD Recommended Positive Control: FG-319M MU319-UC The c-erbB-3 gene product is a 180 kD transmembrane glycoprotein showing tyrosine kinase activity. It belongs to a family of growth receptors that show structural similarity to Epidermal Growth Factor Receptor (EGFR) and the c-erbB-2 proteins. The c-erbB-3 protein is widely expressed in digestive, urinary and respiratory tracts, the circulatory systems, female and male reproductive system but not in hematopoetic system. C-erbB-3 protein has also been seen to be overexpressed in some tumors including those of the breast, stomach, pancreas, colon, and ovary. This antibody stains c-erbB-3 protein in membrane of positive cells. P - Tissue Type FFPE F - Tissue Type Frozen 81 Primary Antibodies Chromogranin A Claudin 1 P LK2H10 Clone: Polyclonal Isotype: IgG1 Kappa Isotype: IgG Source: Mouse Source: Rabbit Immunogen: Tissue from human pheochromocytoma Immunogen: Claudin 1 Specificity: Claudin 1 Specificity: Pancreas tissue stained with Anti-Chromogranin using DAB chromogen P Clone: Secretory storage granules in endocrine cells Localization: Cytoplasm Pre-treatment: None Breast Carcinoma stained with anti-claudin 1 antibody using DAB chromogen Localization: Membrane/Cell junction Pre-treatment: Citra Plus/EZ-AR 1 Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE Ready-to-use (Manual): AM126-5M Ready-to-use (Manual): AR704-5R Ready-to-use (Automated): i6000TM Xmatrx® AM126-10M AX126-YCD Ready-to-use (Automated): i6000TM Xmatrx® AR704-10R AW704-YCD Concentrated: MU126-UC Concentrated: PU704-UP FG-126M Recommended Positive Control: FG-704P Recommended Barrier Control: FB-704P Recommended Positive Control: This antibody recognizes Chromogranin A (68 kD) and other related chromogranin polypeptides from human, monkey, and pig. Chromogranin is widely distributed and through immunohistochemistry, chromogranin has been demonstrated in several elements of the diffuse neuroendocrine system, including anterior pituitary, thyroid parafollicular C cells, parathyroid chief cells, pancreatic islet cells, intestinal enteroendocrine cells, and tumors derived from these cells. The measurement of Chromogranin A has become a valuable tool in the investigation of neuroendocrine neoplasia. This antibody recognizes Chromogranin A (68 kD) and other chromogranin polypeptides in cytoplasm of positive cells. Claudin proteins are a family of proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act a molecular gasket for preventing the leakage of ions, water, etc., between cells. They are abundant in luminial epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different claudin composition. Anti Claudin 1 plays a major role in tight junction specific obliteration of the intercellular space, though calcium independent cell adhesion activity (by similarity), and acts as a coreceptor for HCV entry into hepatic cells. Chromogranin A Claudin-5 P PHE-5 Clone: EP224 Isotype: IgG Isotype: IgG Source: Mouse Source: Rabbit Immunogen: A synthetic peptide corresponding to residues of human Claudin-5 protein Specificity: Claudin-5 protein Specificity: Chromogranin A Localization: Cytoplasm Pre-treatment: AR-10/EZ-AR 1 Manual/i6000: HK058-5K Xmatrx: HX031-YCDE Lung squamous carcinoma stained with anti-Claudin 5 using DAB chromogen Pancreatic islet stained with Anti-Chromogranin A using DAB chromogen Localization: Cell junction/Membrane Pre-treatment: AR Citra Plus/EZ-AR 2 Manual/i6000: HK081-5KE Xmatrx: Ready-to-use (Manual): AM356-5M Ready-to-use (Automated): i6000TM Xmatrx® AM356-10M AX356-YCD Concentrated: Recommended Positive Control: MU356-UC FG-356M Recommended Barrier Control: FB-356M This antibody recognizes Chromogranin A (68 kD) and other related chromogranin polypeptides from human, monkey, and pig. Through immunohistochemistry, chromogranin has been demonstrated in several elements of the diffuse neuroendocrine system, including anterior pituitary, thyroid parafollicular C cells, parathyroid chief cells, pancreatic islet cells, intestinal enteroendocrine cells, and tumors derived from these cells. Chromogranin immunoreactivity was also seen in thymus, spleen, lymph nodes, fetal liver, neurons, the inner segment of rods and cones, the submandibular gland, and the central nervous system. 82 P Clone: P - Tissue Type FFPE F - Tissue Type Frozen Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: HX032-YCDE AN718-5M Recommended Positive Control: AN718-10M AY718-YCD NU718-UC FG-718N Recommended Barrier Control: FB-718N Claudin-5 is a member of the claudin family. Mutations in Claudin-5 have been found in patients with velocardiofacial syndrome. Claudin-5 labels endothelial cells. It has been used as a marker for endothelial lesions. Claudin-5 is also found in bronchial and lung epithelial cells. In tumors, Claudin-5 expression has been found in lung adenocarcinoma and squamous carcinoma. In serous ovarian adenocarcinoma, increased Claudin-5 expression is associated with aggressive behavior. www.biogenex.com Primary Antibodies c-myc Protein Collagen IV P Clone: Clone: COL-94 IgG1 Mouse IgG Isotype: Source: Mouse Source: Immunogen: Peptide AEEQKLISEEDL Immunogen: Human Collagen IV Specificity: Type IV collagen Specificity: c-myc Protein Antigen Localization: Basal laminae/Cyt Localization: Nucleus Pre-treatment: Pre-treatment: AR-10/EZ-AR 2 Pepsin, RT, 30 min./EZ-AR 1/EZ-AR 2 Manual/i6000: EK000-5K Xmatrx: HX031-YCDE/HX032-YCDE Isotype: Breast carcinoma stained with AntiBCA-225 using AEC chromogen Manual: HK058-5K Xmatrx: HX032-YCDE Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Concentrated: AX318-YCD Recommended Positive Control: FG-318M Skin stained with Anti-Collagen IV using AEC chromogen AM318-5M MU318-UC Monoclonal antibody 9E10 reacts with the AEEQKLISEEDL epitope of c-myc protein. The c-myc gene product has been shown, through molecular studies, to be an essential protein for replication of cellular DNA and for the enhancement of mRNA transcription. The activated expression of one of the proto-oncogenes, c-myc, seems to accompany abnormalities in the progression of various malignancies such as lung, breast and colon carcinomas as well as melanomas. The antibody stains c-myc protein in nucleus of positive cells. Collagen III P Skin tissue stained with Anticollagen III using AEC chromogen P 9E10 Ready-to-use (Manual): AM379-5M Ready-to-use (Automated): i6000TM Xmatrx® AM379-10M AX379-YCD Concentrated: MU379-UC This antibody reacts with Collagen IV and does not cross-react with other collagen types. It does not cross-react with human vitronectin, fibronectin or chondroitin sulfate A, B, or C. The positive or negative demonstration of basal lamina using immunostaining helps to distinguish some types of benign lesions from malignant tumors such as tubular carcinoma of the breast. Schwannomas and leiomyomas and their well differentiated malignant counterparts usually immunoreact in a characteristic fashion to the monoclonal antibody for type IV Collagen. The vascular nature of neoplasms such as hemangiopericytoma and epithelioid hemangio-endothelioma can be revealed by type IV collagen with more reliability than other nonspecific stains. This monoclonal antibody stains human Collagen IV in basal laminae. Clone: HWD1.1 Isotype: IgG Source: Mouse Immunogen: Human collagen purified by High Performance Liquid Chromatography Specificity: Collagen type III Localization: ECM Clone: Polyclonal Pre-treatment: Pepsin Source: Rabbit Manual: EK000-5K Immunogen: Xmatrx: None Synthetic peptide from C-terminus of Cyclin D1 Specificity: Cyclin D1 Ready-to-use (Manual): P AM167-5M Ready-to-use (Automated): Xmatrx® AX167-YCD Concentrated: MU167-UC Recommended Positive Control: FG-167M Localization: Nucleus Pre-treatment: AR Citra Plus/EZ-AR 1 Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE Breast cancer tissue stained with Anti-Cyclin D1 using AEC chromogen Collagen type III is a marker for interstitial connective tissue of the extracellular matrix. Collagen type III is diffusely present throughout the interstitial connective tissues making it a better marker than cellular fibronectin, which is more closely associated with basement membrane, and presence in extracellular matrix is minimal. In highly specialized vascular beds of spleen and glomeruli where basement membrane is prominent, little collagen type III is detected, whereas fibronectin is abundant. This antibody does not react with collagens type I, II, IV, V, VI, or VII. This antibody stains positive for Collagen type III in interstitial connective tissue but not on basement membranes. internationalcs@biogenex.com Cyclin D1 Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AR447-5R Recommended Positive Control: FG-447P A447-10R AW447-YCD PU447-UP Cyclins are a family of key regulatory proteins of the cell cycle. Cyclin D1 controls the transition from G1-phase to S-phase of the cell cycle. In addition to breast carcinoma, overexpression is also seen in mantle cell lymphoma, laryngeal epithelial lesions, bladder urothelial tumors, and gastric carcinoma. P - Tissue Type FFPE F - Tissue Type Frozen 83 Primary Antibodies Cyclin D1 Cytokeratin 4 P Clone: Isotype: IgG Source: Rabbit Immunogen: Breast Carcinoma stained with antiCyclin D1 using DAB chromogen P EPR2241(IHC)-32 A synthetic peptide corresponding to residues near C-terminus of human Cyclin D1 Specificity: Cyclin D1 Localization: Nucleus Pre-treatment: EZ-AR™ 1/EZ-AR 2 Manual/i6000: HK521-XAK Xmatrx: HX032-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® AN474-5M AN474-10M AX474-YCD Concentrated: NU474-UC Recommended Positive Control: FG-474N Cyclin D1 belongs to the Cyclin D family. Cyclin D1 is required for the cell cycle G1/S transition. Amplification of overexpression of cyclin D1 plays a pivotal role in the development of various human tumors including parathyroid adenoma, tumor, colon tumor, lymphoma, melanoma and prostate tumor. Cyclin D1 also associates with and regulates the activity of transcription factors, coactivators and corepressors that govern histone acetylation and chromatin remodeling proteins. Esophagus stained with anti-CK4 using DAB chromogen EP454Y Source: Immunogen: Specificity: Localization: Glioblastoma stained with AntiCyclin E2 using AEC chromogen Rabbit Immunogen: A synthetic peptide corresponding to residues on the C-terminus of human CK4 protein Specificity: CK4 Localization: Cytoplasm Pre-treatment: AR Citra Plus/EZ-AR 1 Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE AN717-5M Recommended Barrier Control: FB-717N Cytokeratin 4 (CK4) is a 59 kDa intermediate filament protein associated with cytokeratin 13. It is expressed in suprabasal cells of non-keratinized stratified squamous epithelium. A mutation in the CK4 gene causes white sponge nevus. A decreased expression of CK4 is associated with head and neck squamous carcinoma. It is helpful in differentiation of squamous cell carcinoma of esophagus origin from thyroid origin. P Rabbit A synthetic peptide corresponding to a residue of human Cyclin E2 Cyclin E2 Nuclear Source: Recommended Positive Control: P IgG IgG AN717-10M AY717-YCD NU717-UC FG-717N Cyclin E2 Isotype: EP4 Isotype: Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Cytokeratin 4 Clone: Clone: Cytokeratin 4 positivity in Tonsil stained using DAB Chromogen Clone: 6B10 Isotype: IgG1 Source: Mouse Immunogen: Cytokeratin 4 Specificity: Cytokeratin 4 Localization: Cytoplasm Pre-treatment: Citra plus Manual/i6000: HK087-5KE Xmatrx: None Pre-treatment: EZ-AR™ 1/EZ-AR 2 Manual/i6000: HK521-XAK Ready-to-use (Manual): AM705-5M Xmatrx: HX032-YCDE Ready-to-use (Automated): i6000TM Xmatrx® AM705-10M AY705-YCD Ready-to-use (Manual): AN519-5M Ready-to-use (Automated): i6000TM Xmatrx® AN519-10M AX519-YCD Concentrated: MU705-UC Recommended Positive Control: FG-705M Concentrated: NU519-UC Recommended Barrier Control: FB-705M Recommended Positive Control: FG-519N Cyclin E2 is a member of the cyclin E family that can associate with and activate Cdk2. Expression of cyclin E2 is essential for the control of the cell cycle at the late G1 and early S phase, and this protein may regulate distinct rate-limiting pathways during this phase. Cyclin E2 specifically interacts with Cdk inhibitors of the CIP/KIP family and activates both Cdk2 and Cdk3. Expression of the viral E6 oncoprotein in normal human fibroblasts increases levels of cyclin E2, but not cyclin E1, while expression of the E7 oncoprotein upregulates both. Abnormally high levels of cyclin E expression have frequently been observed in human cancers. 84 P - Tissue Type FFPE F - Tissue Type Frozen RU O Cytokeratin 4 is a 59 kD cytokeratin intermediate fillament protein. It is found in non-complying squamous epithelium such as that of the superficial and intermediate epithelial cells of the esophagus, ectocervix, tongue, vagina, larynx, pharynx, epiglotis and anus, as well as the superficial cells of the cornea. Cytokeratin 4 is also expressed in the superbasal cells of urinary bladder, transitional epithelium in single cells and cell groups of sweat glands, prostatic ducts and in cylindrical, cilliated bronchial epithelial cells. For research use only. Not for use in diagnostic procedures. www.biogenex.com Primary Antibodies Cytokeratin 5 Cytokeratin 5 + Cytokeratin 14 P P Clone: Isotype: Squamous Cell carcinoma stained with Anti-Cytokeratin 5 using DAB chromogen EPR1600Y Clone: EP24 + EP61 IgG Isotype: Source: IgG Rabbit Immunogen: CK5: A synthetic peptide corresponding to residues near the C-terminus of human CK-5 protein CK14: A synthetic peptide corresponding to residues near the C-terminus of human Cytokeratin 14 protein Cytokeratin 5 & 14 Source: Rabbit Immunogen: A synthetic peptide corresponding to residues in the head region of human cytokeratin 5 Specificity: Cytokeratin 5 Localization: Cytoplasm Pre-treatment: AR Citra Plus/EZ-AR 1 Manual: HK081-5KE Xmatrx: HX031-YCDE Ready-to-use (Manual): Prostate stained with anti-CK5&14 using DAB chromogen Specificity: AN484-5M Ready-to-use (Automated): i6000TM Xmatrx® AN484-10M AX484-YCD Concentrated: NU484-UC P Skin stained with Anti-Cytokeratin 5 & 6 using DAB chromogen Cytoplasm Pre-treatment: AR Citra Plus/EZ-AR 2 Manual/i6000: HK081-5KE Xmatrx: Cytokeratin 5 is a high molecular weight cytokeratin found on most basal stratified squamous epithelia. Recent studies show expression for cytokeratin 5 in basal-like breast carcinomas, salivary gland carcinomas, mesotheliomas and ameloblast during enamel growth. Cytokeratin 5 & 6 Localization: Clone: EPR1600Y & EPR1602Y Isotype: IgG Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: HX032-YCDE AN730-5ME Recommended Positive Control: AN730-10ME AY730-YCDE NU730-UCE FG-730N Recommended Barrier Control: FB-730N CK5 labels myoepithelial cells of breast and prostate basal cells. CK5 and calretinin have been useful in mesothelioma and differentiation of adenocarcinomas, especially when facing metastatic tumors of unknown origin. Cytokeratin 14 (CK14) is a 50-kDa keratin expressed in abundance in stratified epithelial, epidermal, basal, mesothelial, and myoepithelial cells in various tissues including breast and prostate. Cytokeratin 5/14-positive breast cancers are true basal phenotype confined to BRCA1 tumors. Along with p63 and CK5, the CK14 antibody has been a useful marker for cells with basal, squamous and myoepithelial differentiation. Source: Rabbit Immunogen: Synthetic peptides corresponding to residues on the N-terminus of human Cytokeratin 5 and cytokeratin 6 respectively Specificity: Cytokeratin 5 & 6 Localization: Cytoplasm Clone: EPR1602Y Pre-treatment: AR Citra Plus/EZ-AR 1 Isotype: IgG Manual: HK08-5KE Source: Rabbit Xmatrx: HX031-YCDE Immunogen: A synthetic peptide corresponding to residues on the N-terminus of human Cytokeratin 6. Specificity: CytoKeratin 6 Ready-to-use (Manual): AN588-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN588-10M AX588-YCD NU588-UC Recommended Positive Control: FG-588N P Skin tissue stained with AntiCytokeratin 6 using DAB chromogen Cytokeratin 5 (58 kD) is expressed in basal, intermediate and superficial cell layers of stratified epithelia as well as transitional epithelia, and in mesothelial cells and mesothelioma. Cytokeratin 6 (56 kD) is also a high molecular weight cytokeratin expressed by proliferating squamous epithelium. Types 5 and 6 of cytokeratin (CK5/6) are in high frequency in mesothelial cells, as opposed to cells of adenocarcinoma. CK5/6 has been shown to be a reliable marker for mesothelioma and squamous cell carcinoma of the lung and Bowen’s disease. It does not react with pulmonary adenocarcinoma. CK5/6 is also used as a confirmatory immunostain to recognize lymphoepitheliomas. internationalcs@biogenex.com Cytokeratin 6 Localization: Cytoplasm Pre-treatment: AR Citra Plus/EZ-AR 1 Manual: HK081-5KE Xmatrx: HX031-YCDE Ready-to-use (Manual): AN500-5M Ready-to-use (Automated): i6000TM Xmatrx® AN500-10M AX500-YCD Concentrated: NU500-UC Recommended Positive Control: FG-500N The cytokeratin 6 (CK6) is expressed in a heterogeneous array of epithelial tissues under normal conditions, but is better known for its strong induction in stratified epithelia that feature an enhanced cell proliferation rate or abnormal differentiation. CK6 shows a complex regulation with constitutive and inducible components in several stratified epithelia, including the oral mucosa and skin. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. P - Tissue Type FFPE F - Tissue Type Frozen 85 Primary Antibodies Cytokeratin 7 Cytokeratin 7 & 8 P P Clone: OV-TL12/30 Clone: OV-TL12/30 & C51 Isotype: IgG1Kappa Isotype: IgG1 Source: Mouse Source: Mouse Immunogen: Ovarian carcinoma cells Immunogen: Specificity: Cytokeratin 7 Ovarian carcinoma cells & MCF-7 cells Localization: Cytoplasm Specificity: Cytokeratin 7 & 8 Pepsin/EZ-AR 2 Localization: Cytoplasm Pre-treatment: AR Citra Plus/EZ-AR 1 Pre-treatment: Breast Carcinoma stained with AntiCytokeratin7 using DAB chromogen Manual/i6000: EK000-5K Xmatrx: HX032-YCDE Ready-to-use (Manual): AM255-5M Ready-to-use (Automated): i6000TM Xmatrx® AM255-10M AX255-YCD Concentrated: MU255-UC Recommended Positive Control: FG-255M Recommended Barrier Control: Breast carcinoma stained with Anti-Cytokeratin 7&8 using DAB chromogen Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE Ready-to-use (Manual): AM587-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM587-10M AX587-YCD Recommended Positive Control: FG-587M MU587-UC FB-255M Cytokeratin 7 is a 54 kD marker of simple epithelium. Antibody to Cytokeratin 7 strongly stains all cell layers of the urinary bladder transitional epithelium. However, Cytokeratin 7 is absent from gastrointestinal epithelium, hepatocytes, proximal and distal tubules of the kidney, and myoepithelium, and also cannot be detected in the stratified epithelia of the skin, tongue, esophagus, or cervix. Cytokeratin 7 recognizes specific subtypes of adenocarcinomas and can be used to differentiate between Cytokeratin 7-positive tissues such as ovarian carcinomas and transitional cell carcinomas and Cytokeratin 7-negative tissues such as carcinomas of the gastrointestinal tract and prostate cancers. Cytokeratins 7 and 8 are two closely related type II cytokeratins characteristic of simple epithelia. Cytokeratin 7 is less widespread than cytokeratin 8 and is expressed in sebaceous and sweat glands and some cells of the inner hair root sheath. Cytokeratin 8 is primarily found in the non squamous epithelia. Cytokeratin 7 is usually present in adenocarcinomas of lung, breast, endometrioid tumors, transitional cell carcinoma of the bladder. The combination of cytokeratin 7 and 8 is a useful marker for differentiating adenocarcinomas and ductal carcinomas from squamous cell carcinomas. Cytokeratin 8 P Cytokeratin 7 P Breast Carcinoma stained with AntiCytokeratin7 using DAB chromogen Clone: EPR1619Y Isotype: IgG C51 Isotype: IgG1 Source: Mouse Immunogen: A cytoskeletal preparation of MCF-7 cells Cytokeratin 8 Source: Rabbit Specificity: Immunogen: A synthetic peptide corresponding to residues near N-tertm of human CK-7 Localization: Cytoplasm Pre-treatment: AR Glyca/EZ-AR 1 Specificity: Cytokeratin 7 Localization: Cytoplasm Pre-treatment: EZ-AR™ 2/ EZ-AR 1 Manual/i6000: HK522-XAK Xmatrx: HX031-YCDE Ready-to-use (Manual): AN528-5M Ready-to-use (Automated): i6000TM Xmatrx® AN528-10M Concentrated: NU528-UC Recommended Positive Control: FG-528N AX528-YCD Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. Keratin 7 (CK7) is expressed in a wide range of epithelial structures in humans. Protein expression levels of CK7 appear significantly higher in chromophobe RCC compared to oncocytoma. CK7 and parvalbumin immunostains may be useful in differentiating oncocytoma from chromophobe RCC in problematic cases. Also, the pattern of immunohistochemical expression of CK7 along with CK 20 is commonly used to assess possible primary sites of metastatic carcinomas. 86 Clone: P - Tissue Type FFPE F - Tissue Type Frozen Breast Carcinoma stained with AntiCytokeratin8 using DAB chromogen Manual/i6000: HK167-5K Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM142-5M AM142-10M AX142-YCD Recommended Positive Control: FG-142M MU142-UC Cytokeratin 8 (52 kD) and 18 (45 kD) comprise a Cytokeratin pair as markers for simple epithelia. In most situations, Cytokeratin 8 exists in tissues together with Cytokeratin 18, but there are exceptions among some normal and abnormal epithelial cells. Therefore, it is useful to use both Cytokeratin 8 and Cytokeratin 18 in combination with other anti Cytokeratin antibody monoclonals when studying cytokeratin expression patterns. Clone C-51 is designed for the specific localization of Cytokeratin 8 and does not cross-react with human cytokeratin numbers 7, 17, 18, or 19. This antibody stains Cytokeratin 8 in cytoplasm of positive epithelial cells. www.biogenex.com Primary Antibodies Cytokeratins 8 & 18 Cytokeratin 13 P 5D3 Clone: AE8 Isotype: IgG1 Isotype: IgG Source: Mouse Source: Mouse Cytokeratins from human breast carcinoma cell line MCF-7 Immunogen: Human epidermal keratin Specificity: Cytokeratin 13 Localization: Cytoplasm Cytokeratins 8 and 18 Pre-treatment: EZ-AR 1 Immunogen: Specificity: Colon carcinoma stained with AntiCytokeratin 8 &18 using DAB chromogen Localization: Cytoplasm Pre-treatment: Pepsin, 37°C, 5 min / EZ-AR 1 Manual/i6000: EK000-5K Xmatrx: HX031-YCDE Ready-to-use (Manual): AM131-10M AX131-YCD Concentrated: MU131-UC Recommended Positive Control: Recommended Barrier Control: FG-131M FB-131M Skin stained with Anti-Cytokeratin 10 using DAB chromogen Isotype: IgG1 None AM132-5M Ready-to-use (Automated): Xmatrx® AX132-YCD Recommended Positive Control: FG-132M Cytokeratins 4/13 are markers for stratified squamous epithelia in internal organs including esophagus and tongue. This antibody is a reliable marker for squamous metaplasia found in respiratory tissue and prostate gland. Squamous metaplasia in the respiratory tract and in some other human organs may be associated with a precancerous condition. This 51 kD Cytokeratin 13, which is expressed in internal non-keratinized stratified squamous epithelia, and its frequently coexpressed partner, the basic 59 kD Cytokeratin 4, may be regarded as markers for esophageal-type differentiation. This antibody stains most cytoplasm in stratified squamous epithelium (except skin epidermis). Cytokeratin 14 P P DEK-10 HX031-YCDE Manual/i6000: Ready-to-use (Manual): Carcinomas may be classified precisely by the analysis of their keratin patterns. Clone 5D3 recognizes an epitope restricted to a few members of the cytokeratin subclasses, specifically Cytokeratins 8 and 18. This antibody reacts with all simple epithelia including glandular epithelium and ciliated pseudostratified columnar epithelium localized in thyroid, female breast, gastrointestinal and respiratory tract. 5D3 may be a useful marker for demonstrating columnar cell differentiation when studying biphasic differentiation of basal cells of respiratory or intermediate epithelium. Clone: Xmatrx: Suprabasal cells in esophagus stained with Anti-Cytokeratin 13 using DAB chromogen AM131-5M Ready-to-use (Automated): i6000TM Xmatrx® Cytokeratin 10 P Clone: Source: Mouse Immunogen: Human epidermal keratin isolated by high salt extraction Specificity: Cytokeratin 10 Squamous cell carcinoma tissue stained with Anti-Cytokeratin 14 using AEC chromogen Clone: LL002 Isotype: IgG1 Kappa Source: Mouse Immunogen: Thyroglobulin conjugated synthetic peptide representing the C-terminal (last 15 residues) of human cytokeratin 14 Specificity: Cytokeratin 14 Localization: Cytoplasm Pre-treatment: AR Citra Plus/EZ-AR 1 Localization: Cytoplasm Pre-treatment: EZ-AR 1 Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE Xmatrx: HX031-YCDE Manual/i6000: None Ready-to-use (Manual): AM201-5M Ready-to-use (Automated): Xmatrx® Concentrated: AX201-YCD MU201-UC Recommended Positive Control: FG-201M Cytokeratins 1 and 10 are expressed only in suprabasal layers, and their expression increases with epidermal maturation. In terminally differentiated keratinocytes of the stratum corneum, Cytokeratins 1 and 10 are regarded as markers for orthokeratinization. Keratinizing areas expressing Cytokeratin 10 have been demonstrated in various well differentiated squamous cell carcinomas derived from epidermis as well as from various internal sites of stratified epithelia. This antibody stains cytoplasm in epithelial cells of the stratum corneum. internationalcs@biogenex.com Ready-to-use (Manual): AM146-5M Ready-to-use (Automated): i6000TM Xmatrx® AM146-10M AX146-YCD Concentrated: MU146-UC Recommended Posiitve Control: FG-146M Cytokeratin 14 (molecular weight 50 kD), an acidic (Type I) cytokeratin protein, is one of the cytokeratin pairs (50/ 58 kD) that distinguishes stratified epithelial cell types from simple epithelial types. Cytokeratin 14 is homogeneously expressed in all cells of the keratinizing squamous epithelium and is confined to the basal and parabasal cells in the nonkeratinizing squamous epithelium of the normal adult urinary tract. The monoclonal antibody to Cytokeratin 14 may be helpful in distinguishing the cell types of the human mammary gland, thus it may also be used to study histogenesis of breast carcinoma. This antibody stains Cytokeratin 14 in cytoplasm of epithelial cells. P - Tissue Type FFPE F - Tissue Type Frozen 87 Primary Antibodies Cytokeratin 15 Cytokeratin 17 P Squamous cell carcinoma stained with Anti-cytokeratin 15 using AEC chromogen EPR1614Y Clone: E27 Isotype: IgG Isotype: IgG Source: Rabbit Source: Mouse Immunogen: A synthetic peptide corresponding to residues on the C-terminus of human CK-15 Immunogen: Recombinant human cytokeratin 17 Specificity: Cytokeratin 17 Localization: Cytoplasm Specificity: Cytokeratin 15 Pre-treatment: EZ-ARTM 2/EZ-AR 1 Localization: Cytoplasm Manual/i6000 HK522-XAK Pre-treatment: EZ-AR™ 1 Xmatrx: HX031-YCDE Manual/i6000 HK521-XAK Xmatrx: HX031-YCDE Ready-to-use (Manual): AN485-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN485-10M AX485-YCD NU485-UC Cytokeratin 15 is subdivided into acidic type I intermediate filament proteins. Cytokeratin 15 has been reported in basal keratinocytes of the epidermis and has also been proposed as marker of stem cells in the hair follicle bulge. Cytokeratin 15 can be used to differentiate limbal phenotype from the conjunctiva. Cytokeratin 16 Squamous Cell carcinoma stained with Anti-CK17 using DAB chromogen Ready-to-use (Manual): AM572-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Recommended Positive Control: AM572-10M AX572-YCD MU572-UC FG-572M Cytokeratin 17 is 46 kD intermediate filament found in simple epithelia sometimes in association with Cytokeratin 7. This antibody has been used to distinguish cervical immature squamous metaplasia from high grade cervical intraepithelial neoplasia (CIN III). Anti-CK17 also labels myoepithelial cells in the benign breast tissue. CK17 labelling of breast carcinoma cells (so-called basal phenotype) has been associated with a poor prognosis. P Squamous cell carcinoma stained with Anti-cytokeratin 16 using AEC chromogen Clone: EP1615Y Isotype: IgG Source: Rabbit Immunogen: A synthetic peptide corresponding to residues on the N-terminus of human CK-16 Cytokeratin 18 P Clone: DC-10 Isotype: IgG1 Source: Mouse Immunogen: A cytoskeletal preparation of HeLa cells Cytokeratin 18 Specificity: Cytokeratin 16 Localization: Cytoplasm Specificity: Pre-treatment: EZ-AR™ 1 Localization: Cytoplasm Manual/i6000: HK521-XAK Pre-treatment: AR Glyca/EZ-AR 1 Xmatrx: HX031-YCDE Ready-to-use (Manual): AN486-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN486-10M AX486-YCD NU486-UC Cytokeratin 16 is a type I keratin (42 KD), observed in psoriatic epidermis, also in normal heel epidermis, trunk epidermis induced hyperproliferate by trauma. It is associated with proliferation. 88 P Clone: P - Tissue Type FFPE F - Tissue Type Frozen Adenocarcinoma stained with AntiCK18 using AEC chromogen Manual: HK167-5K Xmatrx: HX031-YCDE Ready-to-use(Manual): AM143-5M Ready-to-use (Automated): Xmatrx® AX143-YCD Concentrated: MU143-UC Recommended Positive Control: FG-143M Cytokeratins 8 (52 kD) and 18 (45 kD) comprise a cytokeratin pair as markers for simple epithelia. The monoclonal antibodies specific for cytokeratin 18 stain all carcinomas derived from simple epithelia but do not stain well-differentiated squamous cell carcinoma. It is useful to use monoclonal antibodies to Cytokeratins 8 and 18 in combination with other anti-cytokeratin monoclonal antibodies when studying cytokeratin expression patterns. This antibody stains Cytokeratin 18 in cytoplasm of epithelial cells. www.biogenex.com Primary Antibodies Cytokeratin 19 Cytokeratin 20 P Colon carcinoma stained with Anti-Cytokeratin 19 using DAB chromogen P Clone: RCK108 Clone: EPR1622Y Isotype: IgG1 Kappa Isotype: IgG Source: Mouse Source: Rabbit Immunogen: Total cell extract from human bladder cancer cell line Immunogen: A synthetic peptide corresponding to residues on the C-terminus of human Cytokeratin 20 Specificity: Cytokeratin 20 Specificity: Cytokeratin 19 Localization: Cytoplasm Pre-treatment: Pepsin, 37°C, 5 min./ EZ-AR 2 Manual: EK000-5K Xmatrx: HX032-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM246-5M Recommended Positive Control: FG-246M Colon carcinoma stained with Anti-Cytokeratin20 using DAB chromogen P Cytokeratin 20 positivity in colon carcinoma stained using DAB chromogen Cytoplasm Pre-treatment: AR Citra Plus/EZ-AR 1 Manual: HK081-5KE Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM246-10M AX246-YCD MU246-UC Cytokeratin 19 (molecular mass 40 kD) is a marker of simple epithelia. Cytokeratin 19 has been found in mesothelial and mesothelioma cells, ovarian cysts, cystadenomas, and ovarian carcinomas, in adenocarcinomas of the lung and in tumor cells of pulmonary metastases, in the ductal cells of normal pancreas and in pancreatic cancers. It has been shown to be present in the basal layer of non-keratinizing stratified squamous epithelia such as the oral cavity and the ectocervix. Cytokeratin 20 Localization: AN557-5M Recommended Positive Control: AN557-10M AX557-YCD NU557-UC FG-557N Keratins are intermediate filaments expressed by epithelial cells that are involved in cell movement, differentiation and mitosis. Cytokeratin 20 (CK20) is primarily found in the goblet cells and enterocytes of gastric and intestinal mucosa. CK20 is expressed in colorectal, pancreatic, bile duct adenocarcinomas, and transitional cell carcinoma of the bladder. When used in a panel with CK7, CK20 can be particularly useful in identifying colorectal adenocarcinomas and differentiating it from other metastatic carcinomas and also identifying metastatic colorectal adenocarcinomas Cytokeratin Cocktail Clone: IT-Ks20.8 Isotype: IgG 2a Clone: AE1 and AE3 Source: Mouse Isotype: IgG1 Immunogen: Elecrophoretically purified cytokeratin 20 from human intestinal mucosa Source: Mouse Specificity: Cytokeratin 20 Localization: Cytoplasm Pre-treatment: Protease/EZ-AR 1 Manual/i6000: EK002-5K Xmatrx: HX031-YCDE Ready-to-use (Manual): AM315-5M Ready-to-use (Automated): i6000TM Xmatrx® AM315-10M AX315-YCD Concentrated: Recommended Positive Control: MU315-UC FG-315M P Human epidermal keratin Specificity: Cytokeratin Localization: Cytoplasm Pre-treatment: Trypsin, 37°C, 20 min/ EZ-AR 1 Manual/i6000TM: EK001-5K Skin tissue stained with Cytokeratin cocktail AE1 & AE3 using AEC chromogen Cytokeratin 20 (46kD) is relatively less acidic than other type I keratins. This antibody reacts with certain types of carcinomas such as adeno carcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. It does not stain breast, lung and endometrial adenocarcinomas. The differential staining pattern of this antibody makes it very useful for tumor evaluation when used in conjunction with cytokeratin 7 staining. internationalcs@biogenex.com Immunogen: Xmatrx®: HX031-YCDE Ready-to-use (Manual): AM071-5M Ready-to-use (Automated): i6000TM Xmatrx® AM071-10M AX071-YCD Concentrated: MU071-UC Recommended Positive Control: FG-071M Recommended Barrier Control: FB-071M Human cytokeratins (40 kD to 68 kD) are a family of water-insoluble proteins that form a major part of the cytoskeleton of epithelial cells. Immunohistochemical analysis of a large variety of neoplasms has established keratin protein immunohistochemistry as an important aid for classification of epithelial neoplasms. Monoclonal antibodies AE1 and AE3 recognize the acidic and basic subfamilies of cytokeratin, respectively. Thus, the combination of these two antibodies can be used to detect almost all human epithelia. These antibodies show no cross-reactivities with other cytoskeletal proteins. This monoclonal antibody cocktail can be used to detect almost all human epithelia. Membrane and cytoplasmic staining is seen in epithelial cells. P - Tissue Type FFPE F - Tissue Type Frozen 89 Primary Antibodies Cytokeratin Cocktail, Broad Spectrum P P Clone: 34βE12/C51/AE1 Clone: 34βE12 Isotype: IgG1 Isotype: IgG1 Kappa Source: Mouse Source: Mouse Immunogen: Human epidermal keratin and cytoskeletal preparation of MCF-7 cells Immunogen: Human stratum corneum Specificity: High molecular weight cytokeratin Specificity: Normal stomach mucosa showing cytoplasmic positivity for Cytokeratin cocktail using DAB chromogen Cytokeratin, High MW Cytokeratin Localization: Cytoplasm Pre-treatment: Pepsin, 37°C, 5 min./ EZ-AR 2 Manual: EK000-5K Xmatrx®: HX032-YCDE Ready-to-use (Manual): AM273-5M Ready-to-use (Automated): Xmatrx® Recommended Positive Control: AX273-YCD FG-273M Human cytokeratins, which form a major part of the cytoskeleton of epithelial cells, belong to a family of water soluble proteins ranging in size from 40 to 68 kD. Various subsets of cytokeratin proteins occur in any given epithelium, depending on the epithelium cell type, stage of differentiation and embryonic development, cellular growth environment, and type of malignancy. Immunohistochemical analysis of a large variety of neoplasms has established that cytokeratin protein immunohistochemistry is an important aid for epithelial tumor classification. This monoclonal antibody stains keratin in the cytoplasm of positive epithelial cells. Prostatic basal cells stained with Anti-Cytokeratin (HMW) using DAB chromogen Breast Carcinoma stained with AntiCK88 using DAB chromogen Source: Mouse Immunogen: Human epidermal keratin Specificity: Cytokeratin Localization: Cytoplasm Pre-treatment: Pepsin, RT, 10 min. Manual/i6000: EK000-5K/EZ-AR 2 Xmatrx: HX032-YCDE Ready-to-use (Manual): AM372-5M Ready-to-use (Automated): i6000TM Xmatrx® AM372-10M AX372-YCD Concentrated: Manual/i6000: EK000-5K Xmatrx: HX031-YCDE AM291-5M AM291-10M AX291-YCD Recommended Positive Control: FG-291M Recommended Barrier Control: FB-291M Monoclonal antibody 34βE12 is specific for "high molecular weight" cytokeratins 1, 5, 10, 14, corresponding to molecular weights of 68, 58, 56.5, and 50 kD, respectively, which are characteristically found in complex epithelium. The antibody reacts with all squamous and ductal epithelium and stains carcinomas. It reacts with benign small-acinar lesions of the prostate. This antibody stains positive in cytoplasm of epithelial cells. Cytokeratin, High MW (Basic) P LL002+DEK10+RCK108+OVTL12/30+C11 IgG Cocktail Pepsin, 37°C, 20 min/ EZ-AR 1 Ready-to-use (Automated): i6000TM Xmatrx® P Isotype: Cytoplasm Pre-treatment: Ready-to-use (Manual): Cytokeratin Cocktail, Broad Spectrum Clone: Localization: Squamous Cell carcinoma stained with Anti-Cytokeratin using DAB chromogen Clone: AE3 Isotype: IgG Source: Mouse Immunogen: Total keratin was isolated from human epidermal callus. After heating to 65° C for 10 minutes, the denatured keratins were used as the antigen Specificity: Cytokeratin high MW (basic) Localization: Cytoplasm Pre-treatment: Trypsin, 370C, 20 min./ EZ-AR 1 Manual: EK001-5K Xmatrx HX031-YCDE Ready-to-use (Manual): AM133-5M AX133-YCD MU372-UC Ready-to-use (Automated): Xmatrx® Concentrated: Recommended Positive Control: FG-372M Recommended Positive Control: FG-133M Recommended Barrier Control: FB-372M Human cytokeratins, a family of water-insoluble polypeptides, form the major part of the cytoskeleton in all normal and neoplastic epithelial cells. CK88 is a broad spectrum antibody cocktail that reacts with a variety of normal and neoplastic epithelia. It recognizes most epithelium including simple, basal, suprabasal layers, cornea, cornifying stratified epithelium of skin, transitional epithelium of urinary tract, and squamous epithelium. Analysis of intracellular keratin by immunoperoxidase technique is helpful in establishing the epithelial nature of primary or metastatic poorly differentiated neoplasms. This antibody stains cytokeratin in cytoplasm of normal and neoplastic epithelial cells. 90 P - Tissue Type FFPE F - Tissue Type Frozen MU133-UC The cytokeratins are a family of water insoluble proteins (40-70 kD) found in almost all epithelial cell types. Monoclonal cytokeratin antibody AE3 recognizes all basic (Type II) keratins. Since each epithelium contains at least one acidic and one basic keratin, AE3 may be used as a broadly reactive antibody which stains positive for most epithelia and their neoplasms. AE3 has shown great sensitivity and broad specificity for keratins under various conditions of fixation and staining. This antibody stains positive for cytoplasm of most epithelia and their neoplasms. www.biogenex.com Primary Antibodies Cytokeratin, Low MW Cytokeratin, Pan P P Breast Carcinoma stained with AntiCytokeratin using DAB chromogen Clone: AE1 Clone: C11 Isotype: IgG1 Isotype: IgG1 Source: Mouse Source: Mouse Immunogen: Human epidermal keratin Immunogen: Specificity: 40, 48, 50 and 56.5 kD keratins Cytoskeletal proteins from A431 cells Specificity: Cytokeratins Localization: Cytoplasm Localization: Cytoplasm Pre-treatment: Pepsin, RT, 10 min./ EZ-AR 2 Pre-treatment: Protease, RT, 10 min./ EZ-AR 1 Manual/i6000: EK000-5K Xmatrx: HX032-YCDE Ready-to-use (Manual): Cytokeratin Pan on skin tissue stained using AEC chromogen AM075-10M AX075-YCD Concentrated: MU075-UC Recommended Positive Control: Recommended Barrier Control: FG-075M FB-075M The cytokeratins are a family of water insoluble proteins (40-70 kD) found in almost all epithelial cell types. Low molecular weight cytokeratin antibody AE1 has proven to be a widespread histological marker for the restricted staining of the epidermal basal layer of skin and almost all epithelially derived tumors. It can be used as a marker for cells of epithelial origin. This antibody recognizes most type I keratins and shows broad species specificity reacting with keratins of many species including human, rabbit, mouse, bovine, and chick. Staining is usually stronger in alcohol-fixed tissues than in formalin-fixed tissues. Adenocarcinoma stained with Anti-Cytokeratin Pan using DAB chromogen Clone: Lu-5 Isotype: IgG1 Kappa Source: Mouse Immunogen: Cells from a lung cancer cell line Specificity: Cytokeratins Localization: Cytoplasm Pre-treatment: Pepsin, 37°C, 5 min./ EZ-AR 1 HX031-YCDE AM357-5M Ready-to-use (Automated): Xmatrx® AX357-YCD Concentrated: MU357-UC Human keratins are a family of water-insoluble proteins with molecular weights ranging from 40-68kD. This monoclonal cytokeratin antibody can be used to detect cytokeratins 4, 5, 6, 8, 10, 13, and 18 in simple or stratified epithelium in most vertebrates including humans. It can be used as a marker for carcinomas as well as some special types of tumors which have an epithelial component or differentiation. This antibody stains cytokeratin in cytoplasm of normal and malignant epithelial cells in formalin-fixed, paraffin-embedded tissue sections, frozen sections or methanol-acetone-fixed culture cells. Cytomegalovirus (CMV) P Cytokeratin, Pan P EK002-5K Xmatrx: Ready-to-use (Manual): AM075-5M Ready-to-use (Automated): i6000TM Xmatrx® Manual: Clone: BM204 Isotype: IgG1 Source: Mouse Specificity: Cytomegalovirus Localization: Nucleus Pre-treatment: None Colon tissue stained with anti-CMV using DAB chromogen Manual: EK000-5K Ready-to-use (Manual): AM254-5ME Xmatrx: HX031-YCDE Concentrated: MU254-UCE Ready-to-use (Manual): AM181-5M Ready-to-use (Automated): Xmatrx® Concentrated: AX181-YCD MU181-UC Recommended Positive Control: FG-181M RU O Cytomegalovirus (CMV) is a member of the family Herpesviridae. It is found in several body fluids including saliva, urine, breast milk, cervical secretions, blood, and semen. This antibody reacts with an early nonstructural antigen of 68 kD found in the nucleus of infected cells. This antibody stains CMV particles in infected tissues and does not cross-react with the Herpes viruses. For research use only. Not for use in diagnostic procedures. The Lu-5 antibody recognizes an epitope on the surface of cytokeratin filaments which is present in a wide range of cytokeratins, except in intermediate-size filament proteins. This epitope may be found in all human epithelia and carcinomas and is resistant to formalin-fixation. The Lu-5 antibody was determined is a useful pan cytokeratin marker for the detection of both normal and malignant epithelial and mesothelial cells. The Lu-5 antibody stains surface of cytokeratin filaments in a wide variety of normal and tumor tissues. internationalcs@biogenex.com P - Tissue Type FFPE F - Tissue Type Frozen 91 Primary Antibodies Desmin Dystrophin P P Clone: D33 Clone: Dys1 (Dy4/6D3) Isotype: IgG1 Kappa Isotype: IgG2a Source: Mouse Source: Mouse Immunogen: Purified desmin from human leiomyoma Immunogen: Bacterial fusion protein containing mid-rod domain of human dystrophin Specificity: Dystrophin Specificity: Desmin Localization: Cytoplasm Pre-treatment: None Skeletal muscle fibre stained with Anti-Desmin using DAB chromogen Skeletal muscle stained with AntiDystrophin using DAB chromogen Ready-to-use (Manual): AM072-5M Ready-to-use (Automated): i6000TM Xmatrx® AM072-10M AX072-YCD Concentrated: MU072-UC Recommended Positive Control: FG-072M Recommended Barrier Control: FB-072M Desmin is a 56 kD intermediate filament expressed by cells of smooth, skeletal, and cardiac muscle. In myofibrils, desmin is localized in skeletal and cardiac muscle Z lines, in regions of cell-cell juncture, at the site of apposition of the Z line with the plasma membrane, and in cardiac intercalated disks. The specificity of desmin to muscle cells makes it a useful marker in identifying sarcomas derived from smooth and striated muscle cells such as leiomyosarcomas and rhabdomyosarcomas. This antibody does not cross-react detectably with GFAP, keratin, vimentin, or neurofilament. This antibody stains positive in muscle cells. DOG1 Membrane Pre-treatment: AR-10/EZ-AR 1 Manual: HK058-5K Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Recommended Positive Control: Clone: 1.1 Isotype: IgG Source: Mouse Immunogen: A synthetic peptide corresponding to residues in human MUCDOG1 . AM243-5M AX243-YCD FG-243M Dystrophin is the protein product of the Duchenne and Becker muscular dystrophy (DMD/BMD) gene with a relative molecular mass of 400 kD. This monoclonal antibody reacts with an epitope spanning the mid-rod domain between amino acids 1181 and 1388 of human dystrophin. It stains skeletal, cardiac, and smooth muscle dystrophin from normal human membrane in tissue and some animals. Dystrophin P Clone: Dys2 (Dy8/6C5) Isotype: IgG1 Source: Mouse Immunogen: Synthetic polypeptide consisting of the last 17 amino acids at the carboxy terminus of the human dystrophin sequence Specificity: Dystrophin P GIST stained with Anti-DOG1 using DAB chromogen Skeletal muscle stained with AntiDystrophin using AEC chromogen Localization: Membrane Pre-treatment: AR-10/EZ-AR 1 Manual: HK058-5K Xmatrx: HX031-YCDE Specificity: DOG1 Ready-to-use (Manual): AM244-5M Localization: Cytoplasm/Membrane Pre-treatment: AR Citra Plus/EZ-AR 1 Ready-to-use (Automated): Xmatrx® AX244-YCD Manual/i6000: HK081-5KE Recommended Barrier Control: FG-244M Xmatrx: HX031-YCDE Ready-to-use (Manual): AM570-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM570-10M AX570-YCD Recommended Positive Control: FG-570M MU570-UC DOG1 is a cell surface protein selectively expressed in gastrointestinal stromal tumors (GIST). The DOG1 protein shows no homology at the DNA or amino acid level with KIT. DOG1 antibody labels the epithelium of the following organs: breast, prostate, salivary gland, liver, stomach, testis, pancreas, and gallbladder. DOG1 is a useful marker for GISTs, including PDGFRA mutants that fail to express KIT antigen 92 Localization: P - Tissue Type FFPE F - Tissue Type Frozen Dystrophin is the protein product of the Duchenne and Becker muscular dystrophy (DMD/BMD) gene with a relative molecular mass of 400 kD. Antibodies to dystrophin show that DMD individuals lack dystrophin in their muscle cells or that dystrophin is present at very low levels, whereas BMD individuals produce a protein with reduced abundance or abnormal size. This monoclonal antibody reacts with an epitope spanning the mid-rod domain between amino acids 1181 and 1388 of human dystrophin. This antibody stains membrane in skeletal, cardiac, and smooth muscle dystrophin from normal human tissue and some animals. www.biogenex.com Primary Antibodies E-Cadherin EGFR P P Clone: 36 Clone: Polyclonal Isotype: IgG1 Source: Rabbit Source: Mouse Immunogen: Immunogen: C-terminal peptide of human E-cadherin Synthetic peptide encompassing amino 1195 through 1210 of human Epidermal Growth Factor Receptor Specificity: Epidermal Growth Factor Receptor Specificity: E-Cadherin Localization: Membrane Pre-treatment: AR-10/EZ-AR 1 Manual/i6000TM: HK058-5K Xmatrx: Membranous positivity of E-Cadherin on Colon carcinoma stained using DAB chromogen Squamous Cell carcinoma stained with Anti-EGFR using DAB chromogen HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® AM390-10M AX390-YCD Concentrated: MU390-UC Breast carcinoma stained with antiE-cadherin using DAB chromogen EP6 Isotype: IgG Source: Rabbit Immunogen: A synthetic peptide corresponding to residues in the 5th cadherin domain of human E-Cadherin protein. Specificity: E-Cadherin Localization: Membrane Pre-treatment: AR Citra Plus/EZ-AR 2 Manual/i6000: HK081-5KE Xmatrx: HX032-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Recommended Positive Control: Recommended Barrier Control: FB-725N E-Cadherin is a transmembrane glycoprotein that plays an important role in epithelial cell adhesion. In prostate cancers, the expression of E-cadherin is reported to be reduced or absent in comparison with its expression in normal prostate which is uniformly strong. A decreased expression of E-Cadherin is associated with metastatic potential and poor prognosis in breast cancer and esophagus cancer. In combination with p120 Catenin or Cytokeratin, it is useful for the differentiation between ductal (E-Cadherin positive) and lobular (E-Cadherin negative) breast carcinomas. It may also help in diagnosis of mesothelioma. internationalcs@biogenex.com Xmatrx: HX031-YCDE/HX032-YCDE AR335-5RE AW335-YCDE PU335-UPE EGFR (pan) P Squamous Cell carcinoma stained with Anti-EGF Receptor using DAB chromogen AN725-5M AN725-10M AY725-YCD NU725-UC FG-725N EZ-AR1/EZ-AR2 The rabbit antibody to EGFR (LRVAP) reacts with the 170 kD EGFR transmembrane glycoprotein. It binds specifically to the intracellular portion, regardless of phosphorylation state. The extracellular domain binds epidermal growth factor (EGF) as a proliferation signal. The EGFR antibody is made against a sequence which is unique from related tyrosine kinase receptors and hence shows no cross-reactivity. P Clone: Membrane Pre-treatment: Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Concentrated: AM390-5M E-Cadherin (123-kD) is a cell surface glycoprotein responsible for Ca2+dependent intercellular adhesion between epithelial cells. Alterations in the cell-cell adhesion mechanism mediated by E-Cadherin which is lightly associated with alpha catenin may have implications in the metastatic potential of prostate cancer. E-Cadherin may also play a role in adhesion of dendritic epidermal T cells to keratinocytes. Clone 36 may be used to investigate the process of tumor invasion. E-Cadherin Localization: Clone: EP38Y Isotype: IgG1 Source: Rabbit Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Tyr1068 of human EGF receptor. Specificity: Human EGFR Localization: Membrane Pre-treatment: EZ-AR1/EZ-AR2 Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): AN473-5ME Ready-to-use (Automated): i6000TM Xmatrx® AN473-10ME AX473-YCDE Concentrated: NU473-UCE Epidermal growth factor receptor (EGFR) is a 170-kD transmembrane glycoprotein receptor found on a variety of cell types. After a ligand binds to a single-chain EGFR, the receptor forms a dimer that signals within the cell by activating receptor autophosphorylation through tyrosine kinase activity. Autophosphorylation triggers a series of intracellular pathways that may result in ion transport, morphological changes and proliferative activity. P - Tissue Type FFPE F - Tissue Type Frozen 93 Primary Antibodies Ep-CAM Epithelial Membrane Antigen (EMA) P P Clone: E144 Clone: Mc5 Isotype: IgG Isotype: IgG1 Source: Rabbit Source: Mouse Immunogen: A synthetic peptide corresponding to residues in C-terminus (cytoplasmic domain) of human Ep-CAM. Immunogen: Delipidated human milk fat globules Specificity: Epithelial membrane antigen Specificity: Adenoma tissue stained with AntiEpcam using DAB chromogen Epcam(C-term) Localization: Membrane Pretreatment: AR-Citra /EZ-AR 1/ EZ-AR 2 Manual/i6000: HK087-5KE Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): AN489-5M Ready-to-use (Automated): i6000TM Xmatrx® AN489-10M AX489-YCD Concentrated: NU489-UC Recommended Positive Control: FG-489N Epithelial cell adhesion molecule (Ep-CAM) is a monomeric membrane glycoprotein that is expressed on virtually all epithelial cell membranes and on a variety of epithelial carcinomas. Ep-CAM is overexpressed in a variety of human carcinomas and is, therefore, a potential therapeutic target for human solid tumors. Ep-CAM contains an extracellular domain with two epidermal growth factor-like repeats, followed by a cysteine-poor region, which are necessary for the adhesion properties of the molecule. Breast Carcinoma stained with AntiEMA using DAB chromogen Xmatrx: HX031-YCDE Manual/i6000: None Ready-to-use (Automated): i6000TM Xmatrx® AM182-10M AX182-YCD Concentrated: MU182-UC Recommended Positive Control: FG-182M The mucin antigen recognized by Mc5 is a glycosylated molecule with a molecular mass of 400 kD. The sequence to which this antibody binds is Thr-Arg-Pro-Ala-Pro. Although EMA is primarily located in mammary gland epithelium, other normal epithelia (e.g., lung) will also react against EMA antibody. Staining, however, is the strongest in mammary epithelia. The combination of positive staining for keratin with negative EMA can be used to phenotype the above-mentioned epithelial tumors. Epithelial Specific Antigen Clone: MOC-31 Isotype: IgG1 Clone: E29 Source: Mouse Isotype: IgG2a Kappa Immunogen: Source: Mouse Immunogen: Delipidated extract of human cream Cell line from small cell lung carcinoma, CD2 epithelial antigen Specificity: 40 kD epithelial-specific cluster 2 antigen Specificity: EMA Localization: Membrane & Cytoplasm Pre-treatment: EZ-AR 1/EZ-AR 2 Xmatrx: HX031-YCD/HX032-YCD Manual/i6000: None Ready-to-use (Manual): AM057-5M Ready-to-use (Automated): i6000TM Xmatrx® AM057-10M AX057-YCD Concentrated: MU057-UC Recommended Barrier Control: FB-057M Epithelial Membrane Antigen (EMA), also known as milk fat globule membrane protein, is present on the luminal surface of mammary gland epithelium. Although EMA is primarily located in mammary gland epithelium, other normal epithelia (e.g., lung) will also react against EMA antibody. Cells obtained from solid metastases and pleural effusions accompanying a breast cancer will react with EMA antibody. It may also be useful for identification of meningioma. Human colon carcinoma, osteosarcoma, kidney carcinoma, hepatocellular carcinomas, adrenal carcinoma, embryonal carcinoma, liposarcoma, lung carcinoma, and mixed parotid tumor do not stain with EMA antibody. 94 EZ-AR 1 AM182-5M Epithelial Membrane Antigen (EMA) Pancreatic tissue showing positivity for EMA stained using DAB chromogen Membrane & Cytoplasm Pre-treatment: Ready-to-use (Manual): P P Localization: P - Tissue Type FFPE F - Tissue Type Frozen Adenocarcinoma stained with AntiESA using AEC chromogen Localization: Membrane Pre-treatment: AR Glyca/Pepsin Manual/i6000: HK167-5K Xmatrx EK000-10K Ready-to-use (Manual): AM316-5M Ready-to-use (Automated): i6000TM Xmatrx® AM316-10M AX316-YCD Concentrated: MU316-UC Recommended Positive Control: FG-316M Monoclonal antibody MOC-31 recognizes the cluster 2 antigen which is a 40 kD transmembrane glycoprotein present on the membrane of epithelial cells. Since MOC-31 reacts with virtually all normal epithelia and adenocarcinomas but not with mesothelial cells, this antibody can serve as a reliable marker for determining the origin of pleural and peritoneal tumors. This antibody stains a membrane glycoprotein on epithelial cells, but not mesothelial cells. www.biogenex.com Primary Antibodies Epstein-Barr Virus (EBV) Early Antigen Estradiol P F 1108-1 Clone: Polyclonal Isotype: IgG1 Source: Rabbit Source: Mouse Immunogen: Immunogen: Immunoprecipitated EBV early antigens 17-beta-estradiol conjugated to bovine serum albumin. Specificity: Estradiol Specificity: Immunoprecipitated EBV early antigens Clone: Localization: Nucleus/Cytoplasm Pre-treatment: None Breast carcinoma stained with AntiEstradiol using AEC chromogen Cell Culture Slide stained with AntiEBV using AEC chromogen Ready-to-use (Manual): Concentrated: Recommended Positive Control: AM222-5ME MU222-UCE FG-222M Localization: Nucleus Pre-treatment: EZ-AR 1 Manual/i6000: None Xmatrx: HX031-YCDE Ready-to-use (Manual): AR038-5R Ready-to-use (Automated): Xmatrx® Recommended Positive Control: FG-038P AW038-YCD This Epstein-Barr Virus is the causative agent of infectious mononucleosis and is associated with two human neoplasms, Burkitt’s lymphoma and nasopharyngeal carcinoma. Monoclonal antibody 11081 detects the diffuse early antigen of EBV. It specifically recognizes a 55;50 kD protein complex associated with the early antigen of EpsteinBarr Virus (EBV). It is recommended that this antibody be used with alkaline phosphatase label only. Estradiol plays an important role in the genesis and development of human breast cancer and endometrial carcinoma. It is synthesized primarily in the ovary, but also in the placenta, testis, and possibly the adrenal cortex. Estradiol is also produced by testicular Leydig tumors, as well as by Sertoli tumors of the testis and ovary. It is also produced in mammary gland carcinoma, and carcinoma of the adrenal cortex. erbB2 (HER-2/neu) Estrogen Receptor P P Clone: Isotype: IgG Source: Rabbit Immunogen: Breast carcinoma stained with AntiHER-2/neu using DAB chromogen EP1045Y A synthetic peptide corresponding to residues near the C-terminus of human HER-2 Specificity: HER-2 Localization: Membrane Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Recommended Positive Control: Breast carcinoma stained with AntiER using DAB chromogen 1D5 Isotype: IgG1 Source: Mouse Immunogen: Recombinant estrogen receptor protein Specificity: Estrogen receptor protein Localization: Nucleus Pre-treatment: AR Citra Plus/EZ-AR 2 Manual/i6000: HK081-5KE Xmatrx: HX032-YCDE Ready-to-use (Manual, 3 mL): Ready-to-use (Automated): i6000TM Xmatrx® AN471-5ME AN471-10ME AX471-YCDE FG-471N ErbB-2/Her-2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases. The kinase activity of ErbB2 can be activated without ligand if it is over expressed, and by association with other ErbB proteins. Over expression of ErbB2 is detected in almost 40% of human breast cancers. Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation. ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas. internationalcs@biogenex.com Clone: Concentrated: Recommended Positive Control: Recommended Barrier Control: AM272-2ME AM272-10ME AX272-YCDE MU272-UCE FG-272M FB-272M The determination of steroid hormone receptors has become widely used in the management of hormone-dependent cancers. The Estrogen Receptor content of breast carcinoma is a tool for both prognosis and response to endocrine therapy. Individuals whose tumors contain estrogen receptor are more likely to respond to endocrine manipulation than those whose tumors are estrogen receptor poor. Estrogen Receptor (ER) modulates cellular events by binding estrogen response elements (ERE) when bound to estrogen in dimers. This results in the transcription of a number of proteins, including ER. Protein synthesis occurs in the cytoplasm, after which ER associates with chaperone proteins and is translocated to the nuclear membrane. The ER protein is transported into the nucleus through utilization of ATP. P - Tissue Type FFPE F - Tissue Type Frozen 95 Primary Antibodies Estrogen Receptor (InSite® ER) Estrogen Receptor P Breast carcinoma stained with AntiER-Alpha using DAB chromogen P Clone: EP1 Clone: ER88 Isotype: IgG Isotype: IgG1 Source: Rabbit Source: Mouse Immunogen: Recombinant Estrogen Receptor protein Immunogen: Recombinant Estrogen Receptor protein Specificity: Estrogen receptor protein Specificity: Estrogen receptor protein Localization: Nucleus Localization: Nucleus Pre-treatment: AR Citra Plus Pre-treatment: AR Citra Plus/EZ-AR 1 Manual/i6000: HK081-5KE Xmatrx: None Breast carcinoma stained with AntiER using DAB chromogen Manual/i6000: HK081-5KE Xmatrx HX031-YCDE Ready-to-use (Manual): AN710-5ME Ready-to-use (Manual): AM368-5ME Ready-to-use (Automated): i6000TM Xmatrx® AN710-10ME AY710-YCDE Concentrated: Recommended Positive Control: NU710-UCE FG-710N Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM368-10ME AX368-YCDE MU368-UCE Recommended Positive Control: FG-368M Recommended Barrier Control: FB-710N Recommended Barrier Control: FB-368M Estrogen Receptor-Alpha (ER Alpha) is a nuclear protein and member of the steroid hormone receptor family. ER Alpha possess both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes. Ligand-dependent dimerization and phosphorylation both function to regulate the transcriptional activation of ER alpha. This antibody stains nucleus of neoplastic cells in the breast ductal carcinoma tissues by immunohistochemical techniques. Estrogen receptor (ER) content of breast cancer tissue is an important parameter in the prediction of prognosis and response to endocrine therapy. Highly specific monoclonal antibodies to ER have allowed the determination of receptor status of breast tumors to be carried out. This antibody stains the nucleus of receptor positive cells. Estrogen Receptor-Beta (ER-β) P ER Alpha P Breast carcinoma stained with AntiER-Alpha using AEC chromogen Clone: EPR703(2) Isotype: IgG Source: Rabbit Immunogen: A synthetic peptide corresponding to residues on the Cterminus of human ER alpha Specificity: ER Alpha Localization: Nucleus Pre-treatment: EZ-AR 2 Manual/i6000: None Xmatrx: HX032-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® AN509-5ME Concentrated: NU509-UCE Recommended Positive Control: FG-509M AN509-10ME AX509-YCDE Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes. Ligand-dependent dimerization and phosphorylation both function to regulate the transcriptional activation of ER alpha . The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to antiestrogen (e.g. tamoxifen) therapy. 96 P - Tissue Type FFPE F - Tissue Type Frozen Breast carcinoma stained with AntiER-beta using DAB chromogen Clone: Polyclonal Source: Rabbit Immunogen: A 17-mer sequence close to carboxy-terminus of ER-β protein was chosen to be the template for synthesis of a 4-branch Multiple Antigenic Peptide (MAP) Specificity: Estrogen Receptor-β protein Localization: Nucleus Pre-treatment: AR Citra Plus /EZ-AR 1/ EZ-AR 2 Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® AR385-5R AR385-10R AW385-YCD Concentrated: PU385-UP Human Estrogen Receptor beta (ERb) is highly homologous to human ERa and displays 96% and 58% homology in the DNA and hormone binding domains, respectively. Human ERb mRNA is expressed in testis, prostate, thyroid, ovary, and smooth muscle. ERb is expressed in various normal and neoplastic cells. The rabbit polyclonal antibody ERb88 is directed against human ERb protein and marks nuclei of many different cells on formalin-fixed, paraffin-embedded tissue sections. www.biogenex.com Primary Antibodies Factor VIII-Related Antigen Factor XIII A P P Clone: F8 2.2.9 Clone: EP3372 Isotype: IgG1 Kappa Isotype: IgG Source: Mouse Source: Rabbit Immunogen: Purified Human Factor VIII Immunogen: Specificity: Factor VIII-related antigen Localization: Cytoplasm A synthetic peptide corresponding to residues in human Factor XIII A. Specificity: Factor-XIII A Pre-treatment: Pepsin/EZ-AR 1 Manual/i6000: EK000-5K Xmatrx: HX031-YCDE Bone Marrow stained with AntiFactor XIII A using DAB chromogen Leiomyoma stained with Anti-FVIII using AEC chromogen Ready-to-use (Manual): Localization: Cytoplasm Pre-treatment: EZ-AR™ 1 Manual/i6000: HK521-XAK Xmatrx: HX031-YCDE AM016-5M Ready-to-use (Manual): AN516-5M Ready-to-use (Automated): i6000TM Xmatrx® AM016-10M AX016-YCD Ready-to-use (Automated): i6000TM Xmatrx® AN516-10M AX516-YCD Concentrated: MU016-UC Recommended Positive Control: Concentrated: FG-016M NU516-UC Recommended Barrier Control: FB-016M Recommended Positive Control: FG-516N This antigen has proven to be one of the best available immunohistochemical markers for the identification of endothelial cells. Demonstration of Factor VIII-related antigen by immunohistochemical staining has been suggested to identify vascular invasion by neoplasms. Factor XIII is activated by thrombin and calcium ions to transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine crosslinks between fibrin chains, thus stabilizing the fibrin clot. Also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Fascin Factor XIII Subunit A P Factor XIII A positivity in placenta stained using AEC chromogen Clone: P E980.1 Clone: FCN01 Isotype: IgG Isotype: IgG1 Source: Mouse Source: Mouse Immunogen: Immunogen: Prokaryotic recombinant protein corresponding to a portion of the C-terminus of factor XIIIa molecule Fascin purified from HeLa cells Specificity: Fascin Specificity: Coagulation Factor XIIIa Localization: Cytoplasm Pre-treatment: AR Citra Plus/EZ-AR 1 Manual/i6000: HK081-5KE Xmatrx HX031-YCDE Ready-to-use (Manual): AM337-5M Ready-to-use (Automated): i6000TM Xmatrx® AM337-10M AX337-YCD Concentrated: MU337-UC Recommended Positive Control: FG-337M Cytoplasm Pre-treatment: AR-Citra/EZ-AR 1 Manual/i6000: HK087-5KE Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® AM488-5M Concentrated: MU488-UC AM488-10M AX488-YCD Recommended Positive Control: The enzyme Factor XIII is a protransglutaminase involved in the final part of the coagulation pathway, stabilizing clot formation by crosslinking fibronectin to collagen. Factor XIII is found within a variety of dendritic cells in connective tissues. It plays a general role in various processes such as cell proliferation and tissue remodeling, including embryonic and fetal embryogenesis, wound healing, atherosclerosis, and tumor growth. This antibody stains the cytoplasm of positive cells. internationalcs@biogenex.com Lymph Node stained with AntiFascin using DAB chromogen Localization: FG-488M Human fascin is a highly conserved actin-bundling protein. Fascin, encoded by the human homolog for the sn (hsn) gene, has been localized to microspikes and stress fibers of cultured cells where it is thought to be involved in the formation of microfilament bundles. It is expressed predominantly in dendritic cells. Lymphoid cells, myeloid cells and plasma cells are negative. However. Reed Sternberg cells in Hodgkin’s lymphoma are positive for fascin staining. Epstein-Barr virus may induce expression of fascin in B cells. P - Tissue Type FFPE F - Tissue Type Frozen 97 Primary Antibodies Fibronectin Gastrin P P Clone: Clone: Polyclonal Rabbit IgG Source: Source: Rabbit Immunogen: Immunogen: A recombinant protein Synthetic human Gastrin-I bound to keyhole limpet hemocyanin (KLH) with carbodiimide Gastrin Isotype: Placenta tissue stained with AntiFibronectin using DAB chromogen F14 Specificity: Fibronectin Localization: Cytoplasmic and extracellular matrix staining Specificity: Localization: Cytoplasm Pre-treatment: AR Citra Plus /EZ-AR 1/ EZ-AR 2 Pre-treatment: EZ-AR 1 Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): Stomach tissue stained with AntiGastrin using DAB chromogen AN569-5M Ready-to-use (Manual): AR019-5R Ready-to-use (Automated): i6000TM Xmatrx® AN569-10M AX569-YCD Ready-to-use (Automated): Xmatrx® Concentrated: AW019-YCD PU019-UP Concentrated: NU569-UC Recommended Positive Control: FG-019P Recommended Positive Control: FG-569N Fibronectin is a large heterodimeric glycoprotein composed of two 250kD subunits. It can be found soluble in plasma or insoluble as an extracellular matrix protein. Fibronectin is involved in various cell processes such as cell adhesion, migration, wound healing, and in embryogenesis. The abnormal expression of fibronectin has been implicated with the progression of several cancers. Cellular fibronectin is widely distributed in the stroma of malignant tumours. In non-small cell lung carcinomas (NSCLC), fibronectin expression is upregulated leading to increased tumor growth and apoptosis resistance. The major source of Gastrin in the body is the antropyloric mucosa of the stomach. Significant increases in the antropyloric G-cell (gastrin producers) population occur in a wide variety of clinical conditions such as atrophic gastritis, pernicious anemia, gastric carcinoma, gastric outlet obstruction, Zollinger-Ellison syndrome, and duodenal ulcer disease. Neoplastic proliferations of the gastrin producing cells are frequently associated with the Zollinger-Ellison syndrome. GCDFP-15 P Follicle Stimulating Hormone (FSH) P Clone: Source: Rabbit Immunogen: Human pituitary FSH peptide Specificity: Follicle stimulating hormone (FSH) IgG Source: Rabbit Immunogen: A synthetic peptide corresponding to residues near the N-terminus of human GCDFP-15 Specificity: GCDFP-15 Breast Carcinoma stained with AntiGCDFP-15 using DAB chromogen Localization: Cytoplasm Pre-treatment: EZ-AR™ 1 HK521-XAK Localization: Cytoplasm Pre-treatment: EZ-AR 1 Manual/i6000: Xmatrx: HX031-YCDE Xmatrx: AR026-5R AW026-YCD Malignant pituitary adenomas or pituitary carcinomas arise from and consist of adenohypophysial cells. They can produce various hormones such as ACTH, growth hormone, TSH, FSH, LH, and prolactin. Tumors that do not consist of adenohypophysial cells neither produce nor contain pituitary hormone, and thus immunoperoxidase techniques are helpful in distinguishing them from those pituitary tumors that store various hormones in the cell cytoplasm. FSH, a glycoprotein hormone, stimulates the graafian follicles of the ovary and assists subsequently in follicular maturation and the secretion of estradiol. In males, it stimulates the epithelium of the seminiferous tubules and is partially responsible for inducing spermatogenesis. This antibody stains of FSH in gonadotrophs. 98 EP1582Y Isotype: Polyclonal Pituitary tissue stained with Anti-FSH using AEC chromogen Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Clone: P - Tissue Type FFPE F - Tissue Type Frozen HX031-YCDE Ready-to-use (Manual): AN481-5M Ready-to-use (Automated): i6000TM Xmatrx® AN481-10M AX481-YCD Concentrated: NU481-UC Recommended Positive Control: FG-481N The androgen and prolactin responsive prolactin-inducible protein (PIP)/gross cystic disease fluid protein (GCDFP-15) is expressed in human breast tumors and in such normal exocrine organs as sweat, salivary and lacrimal glands. The hormonally responsive prolactininducible protein (PIP) gene is expressed in breast tumor tissues and in such normal exocrine organs as sweat, salivary and lacrimal glands. www.biogenex.com Primary Antibodies Glomerular Epithelial Protein 1 (GLEPP-1) Glial Fibrillary Acidic Protein (GFAP) P P Clone: GA-5 Clone: 5C11 Isotype: IgG1 Isotype: IgG2b Source: Mouse Source: Mouse Immunogen: GFAP isolated from porcine spinal cord Immunogen: GLEPP-1 fusion protein Glial fibrillary acidic protein (GFAP) Specificity: GLEPP1 Localization: Membrane AR-10/EZ-AR 1/EZ-AR 2 Specificity: Localization: Cytoplasm Pre-treatment: Pre-treatment: None Manual: HK058-5KE Xmatrx: HX031-YCDE/HX032-YCDE Astrocytes and other acidic fibers in cerebrum stained with Anti-GFAP using DAB chromogen Kidney tissue stained with AntiGLEPP-1 using AEC chromogen Ready-to-use (Manual): AM020-5M Ready-to-use (Automated): i6000TM Xmatrx® AM020-10M AX020-YCD Concentrated: MU020-UC Recommended Positive Control: FG-020M Recommended Barrier Control: FB-020M AM336-5M Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Concentrated: Glial Fibrillary Acidic Protein (GFAP) is the subunit of the glial specific “intermediate” filament that includes desmin filaments in smooth muscle, vimentin filaments in cultured fibroblasts, keratin filaments in epithelium and neurofilaments in neural cells. This antibody stains human GFAP in positive astrocytes and other positive cells. AX336-YCD MU336-UC Glomerular epithelial protein 1 (GLEPP1) is a transmembrane protein tyrosine phosphatase found only in the epithelial cells of the renal glomerulus.The monoclonal antibody 5C11 has been raised against a fusion protein made from part of its extracellular domain. In the normal glomerulus, GLEPP1 is present only in visceral glomerular cells (podocytes). The presence of GLEPP1 may be used as a marker of podocyte integrity in various forms of glomerular injury. This antibody stains human GLEPP1 protein in the epithelial cells of the renal glomerulus. Glucagon P Glial Fibrillary Acidic Protein (GFAP) Clone: Polyclonal Polyclonal Source: Rabbit Source: Rabbit Immunogen: Immunogen: GFAP isolated from bovine spinal cord. Synthetic human glucagon Specificity: Glucagon P Clone: Specificity: Cerebellum tissue stained with AntiGFAP using DAB chromogen Glial fibrillary acidic protein (GFAP) Localization: Cytoplasm Pre-treatment: EZ-AR 1 Manual/i6000: None Xmatrx: HX031-YCDE Ready-to-use (Manual): AR020-10R AW020-YCD Concentrated: PU020-UP Recommended Positive Control: FG-020P Glial Fibrillary Acidic Protein (GFAP) is the subunit of the glial specific “intermediate” filament that includes desmin filaments in smooth muscle, vimentin filaments in cultured fibroblasts, keratin filaments in epithelium and neurofilaments in neural cells. This antibody stains GFAP from many species including human, mouse, and rat in cytoplasm of astrocytes and Bergmann glia. internationalcs@biogenex.com Cytoplasm Pre-treatment: EZ-AR 1 Xmatrx: HX031-YCDE Pancreas tissue stained with AntiGlucagon using AEC chromogen AR020-5R Ready-to-use (Automated): i6000TM Xmatrx® Localization: Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Concentrated: AR039-5R AW039-YCD PU039-UP Recommended Positive Control: FG-039P Glucagon is a polypeptide of 29 amino acids produced by the pancreatic alpha cells. In addition to its well known effect of elevating blood glucose concentration, glucagon functions to inhibit gastric and pancreatic secretions. Tumors producing large amounts of glucagon are referred to as glucagonomas. This antibody stains the cytoplasm in A cells of the endocrine pancreas and reacts with glucagon in a number of mammalian species. P - Tissue Type FFPE F - Tissue Type Frozen 99 Primary Antibodies Glut-1 Glypican-3 (GPC3) P Kidney tissue stained with AntiGlut-1 using DAB chromogen SPM498 Clone: GPC3-88 Isotype: IgG Isotype: IgG Source: Mouse Source: Mouse Immunogen: Glut-1 purified from Primary cultures of myoblasts. Immunogen: Glypican-3 is a mouse monoclonal antibody derived from cell culture supernatant Glypican Specificity: GLUT-1 Localization: Membrane/Cytoplasm Specificity: Pre-treatment: EZ-AR™ 2/EZ-AR 1 Localization: Cytoplasm/Membrane Pre-treatment: AR Citra Plus Manual/i6000: HK081-5KE Manual/i6000 : Xmatrx: HK522-XAK HX031-YCDE/HX032-YCDE Ready-to-use (Manual): AM505-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM505-10M AX505-YCD MU505-UC Recommended Positive Control: FG-505M Glucose is fundamental to the metabolism in mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF-1 induced signaling. GLUT-1 is detectable in many human tissues including those of the colon, lung, stomach, esophagus, and breast. GLUT-1 immunoreactivity in some cancers, including trans carcinoma of the urinary bladder, has been associated with aggressive behavior. Glutathione S-Transferase Pi (GST Pi) P GST Pi positivity in breast carcinoma stained using DAB chromogen P Clone: Hepatocellular carcinoma stained with Anti-Glypican-3 using DAB chromogen Ready-to-use (Manual): Ready-to-use (Automated): i6000TM AM539-5M Concentrated: MU539-UC Recommended Positive Control: FG-539M AM539-10M Granulocyte Clone: Polyclonal Source: Rabbit Clone: BM-2 Immunogen: Purified proteins from the cytosol of a human chronic lymphoblastic spleen Isotype: IgG1 Source: Mouse Immunogen: Nuclei from pokeweed mitogen-stimulated human peripheral blood lymphocytes Specificity: Granulocytes Localization: Cytoplasm Specificity: Glutathione S-transferase pi Localization: Nucleus & Cytoplasm Pre-treatment: EZ-AR 1/EZ-AR 2 Xmatrx: HX031-YCDE/HX032-YCDE AR249-5R P Bone marrow trephine stained with Anti-Granulocyte using AEC chromogen AW249-YCD PU249-UP Concentrated: MU210-UC Recommended Positive Control: FG-249P Recommended Positive Control: FG-210M Glutathione S-Transferases (GSTs) are a multigene family of enzymes centrally involved with drug metabolism and detoxification. All eukaryotic species possess multiple cytosolic and membranebound GST isoenzymes, each of which displays distinct catalytic as well as noncatalytic binding properties. P - Tissue Type FFPE F - Tissue Type Frozen O Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. GPC3 belongs to the glypican family of heparan sulfate proteoglycans. This protein may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. Glypican-3 is thought to regulate tissue and organ growth through interactions with growth factors such as insulin-like growth factor II or fibroblast growth factor 2. For research use only. Not for use in diagnostic procedures. Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Concentrated: 100 RU The BM-2 antibody can provide important differentiation information and may be used along with antibodies BM-1 and BM-3 to stain early precursor and mature forms of human myeloid cells. This group of monoclonal antibodies reacts with antigenic determinants present in normal myeloid cells and leukemias of similar derivation. BM-2 recognizes an antigen present in the cytoplasm of mature granulocytes. This antibody stains the cytoplasm of human granulocytes (polymorphonuclear leukocytes) residing in lymphoid and non-lymphoid tissue. www.biogenex.com Primary Antibodies Growth Hormone (hGH) Heat Shock Protein 27 (HSP 27) P P Polyclonal Clone: G3.1 Isotype: IgG Isotype: IgG1 Source: Rabbit Source: Mouse HGH Immunogen: Balb/c mice were immunized with "24K" protein isolated from the cytosol of MCF-7 cells. Spleen cells from immunized mice were fused with NS-1 myeloma cells Specificity: hsp27 Clone: Immunogen: Placenta stained with anti-HGH antibody using DAB chromogen Specificity: HGH Localization: Cytoplasm Pre-treatment: EZ-AR 1 Manual/i6000: HK521-XAK Xmatrx: HX031-YCDE Ready-to-use (Manual): AR707-5R Ready-to-use (Automated): i6000TM Xmatrx® : AR707-10R AW707-YCD Concentrated: PU707-UP Recommended Positive Control: FG-707P Recommended Barrier Control: FB-707P Breast carcinoma stained with AntiHSP27 using AEC chromogen Growth Hormone (GH, somatotropin) is the primary hormone responsible for regulating overall body growth and is also important in organic metabolism. It is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human GH has a molecular weight of 22 kD. GH stimulates growth indirectly by promoting the liver’s production of somatomedins, which act directly on bone and soft tissue to cause growth. GH exerts direct metabolic effects on the liver, adipose tissue and muscle. In general, growth hormone enhances protein synthesis, conserves carbohydrates and uses up fat stores. Localization: Cytoplasm Pre-treatment: AR Citra Plus/EZ-AR 1 Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE Ready-to-use (Manual): AM171-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM171-10M AX171-YCD MU171-UC Recommended Positive Control: FG-171M Recommended Barrier Control: FB-171M HSP27 also known as the 24K estrogen-regulated protein or HSP28, is a small heat shock protein that has been shown to correlate with the expression of estrogen-receptors. Increased levels of HSP27 have been shown to correlate with the presence of ER and PR in human breast tumor biopsy samples. This antibody stains estrogen regulated heat shock protein (HSP27) in cytoplasm of cells in female reproductive tract. Heat Shock Protein (HSP-70) P Helicobacter pylori Clone: BRM-22 Isotype: IgG1 Clone: Polyclonal Source: Mouse Source: Rabbit Immunogen: Bovine brain HSP70 Immunogen: Heat killed bacteria Specificity: Heat Shock Protein70 (HSP-70) Specificity: Helicobacter pylori Localization: Cytoplasm P Pre-treatment: EZ-AR 1/EZ-AR 2 Xmatrx: HX031-YCDE/HX032-YCDE Breast carcinoma stained with AntiHSP 70 using DAB chromogen Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Concentrated: AM289-5M Recommended Positive Control: FG-289M Bacterium Pre-treatment: AR 10/EZ-AR 1/EZ-AR 2 Manual: HK058-5K Xmatrx: HX031-YCDE/HX032-YCDE Infected mucosa stained with Anti-H. pylori using AEC chromogen AX289-YCD MU289-UC HSP-70 is a member of a multigene family encoding several closely related 70-73 kD stress proteins (the HSP-70 family). These genes differ in their intracellular location and regulation and are thought to be involved in protein-protein interactions such as those of the protein products of the p53 tumor suppressor gene and the human c-myc oncogene. This antibody stains HSP-70 localized in the cytoplasm and/ or nuclei in tissue from breast carcinoma, brain tumors, Alzheimer's disease and alcoholic liver disease. internationalcs@biogenex.com Localization: Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® AR442-5RE AR422-10RE AW442-YCDE Concentrated: PU442-UPE Recommended Positive Control: FG-442P H. pylori is associated with B-type gastritis and some duodenal ulcers. Studies conducted by various researchers have shown that immunostaining for H. pylori with monoclonal antibodies is more reliable and easier to read than traditional H&E staining and significantly more sensitive than the Warthin-Starry silver stain, which is very complicated to perform. This antibody stains the bacilli in lumen of infected stomach in formalin-fixed, paraffin-embedded tissue sections. P - Tissue Type FFPE F - Tissue Type Frozen 101 Primary Antibodies Hemoglobin A Herpes Simplex Virus Type I (HSV I) P P Clone: Polyclonal Clone: Polyclonal Source: Rabbit Source: Rabbit Immunogen: Purified hemoglobin Immunogen: Specificity: Hemoglobin A Localization: Cytoplasm Pre-treatment: EZ-AR 1 Rabbit cornea cells infected with the MacIntyre strain of HSV type I and solubilized in detergent Xmatrx: HX031-YCDE Specificity: Herpes Simplex Virus (HSV) type I Infected lung tissue stained with Anti-HSV I using AEC chromogen Placenta tissue stained with Anti-Hb using AEC chromogen Ready-to-use (Manual): AR021-5R Ready-to-use (Automated): Xmatrx® Recommended Positive Control: Localization: Nucleus/Cytoplasm Pre-treatment: EZ-AR 1/EZ-AR 2 Xmatrx: HX031-YCDE/HX032-YCDE AW021-YCD Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® AW084-YCDE FG-021P Concentrated: PU084-UPE Immunohistochemical localization of hemoglobin is an excellent marker for the detection of immature, dysplastic, and megaloblastic erythroid cells particularly in myeloproliferative disorders such as erythroleukemia. Myeloid cells, lymphoid cells, plasma cells, histiocytes and megakaryocytes do not give positive staining for hemoglobin. Megaloblastic erythroid cells give strong staining for hemoglobin. This antibody stains human hemoglobin A predominantly in cytoplasm of erythroid cells. Hepatitis B Virus Core Antigen (HBcAg) AR084-5RE Human herpes simplex virus type I (HSV-I) is part of the Herpesvirus family which also includes HSV-II, Epstein-Barr virus (mononucleosis), herpes zoster (chicken pox) and cytomegalovirus. They grow in the cell nuclei, bud through the nuclear membrane and cause latent infections. HSV are among the most common infectious agents of man, usually transmitted through close personal contact. HSV-I is responsible for causing gingivostomatitis or “fever blisters.” There is a significant degree of cross-reactivity between HSV-I and HSV-II. No cross-reactivity is seen with the Epstein-Barr virus, cytomegalovirus or herpes zooster virus. P Clone: Polyclonal Source: Rabbit Immunogen: HBcAg purified from lysates of E. coli clones containing the viral core DNA Herpes Simplex Virus Type II (HSV II) Specificity: Hepatitis B core antigen Localization: Nucleus P Liver tissue stained with Anti-HBcAg using AEC chromogen Ready-to-use (Manual): AR082-5RE Concentrated: PU082-UPE Recommended Positive Control: Recommended Barrier Control: FG-082P FB-082P Cultured cells infected with HSV II, stained with Anti HSV II using DAB chromogen RU O HBcAg is the designation given to the antigenic activity of the 28 nm nucleocapsid core of Hepatitis B Virus. Immunocytochemical staining for HBcAg can provide conclusive evidence for a viral etiology in hepatitis. Furthermore, viral antigenic expression in liver cells can be correlated with the histopathologic changes, thus facilitating investigation into the mechanism of virus-induced injury. HBcAg is not detected in the serum of either acutely or chronically infected individuals. As the patient becomes symptomatic, HBc antibody becomes detectable. This antibody stains Hepatitis B Virus Core Antigen in nuclei of infected cells in tissue sections stained by immunohistochemical techniques. For research use only. Not for use in diagnostic procedures. 102 P - Tissue Type FFPE F - Tissue Type Frozen Clone: Polyclonal Source: Rabbit Immunogen: HSV type II (strain MS) infected whole rabbit cornea cells solubilized in detergent Specificity: Herpes simplex Virus (HSV) type II Localization: Nucleus/Cytoplasm Pre-treatment: EZ-AR 1 Xmatrx: HX032-YCDE Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® AR085-5RE AW085-YCDE Concentrated: PU085-UPE HSV II is the virus known for causing Herpes genitalis. The antibody reacts with all the major glycoproteins present in the viral envelope and at least one core protein as determined by crossed immunoelectrophoresis. It does not cross react with cytomegalovirus and Epstein-Barr virus. This antibody detects HSV in human cytological samples obtained from superficial lesions or biopsies and infected tissue cultures. www.biogenex.com Primary Antibodies HLA-DR Human Chorionic Gonadotropin (hCG) Beta P Thyroid tissue stained with AntiHLADR using DAB chromogen P Clone: LN3 Clone: M94138 Isotype: IgG2a Isotype: IgG Source: Mouse Source: Mouse Immunogen: Activated human peripheral blood mononuclear cells Immunogen: Purified hCG Beta-subunit Specificity: Beta-hCG Specificity: LN3 Localization: Membrane Pre-treatment: EZ-AR 1/EZ-AR 2 Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): Placenta tissue stained with AntihCG beta using DAB chromogen Localization: Cytoplasm Pre-treatment: AR Citra Plus/EZ-AR 1 Manual HK081-5KE Xmatrx HX031-YCDE AM154-5ME Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® AX154-YCDE Ready-to-use (Automated): Xmatrx® AX395-YCD Concentrated: MU154-UCE Concentrated: MU395-UC Recommended Positive Control: FG-154M RU O HLA-DR is a transmembrane glycoprotein composed of an alpha chain (36 kD) and a beta chain (27 kD). LN3 is reactive with a nonpolymorphic antigen of the HLA-DR (Ia) region, expressed primarily by antigen presenting cells, B-cells of the germinal centers and mantle zones, and additionally by monocytes, macrophages and interdigitating histiocytes. LN3 will produce medium intensity staining on B lymphocytes of germinal centers and mantle zones, and high intensity staining of interdigitating histiocytes in T-cell zones. This antibody stains the HLA-DR antigen in membrane of positive cells. For research use only. Not for use in diagnostic procedures. HSA AM395-5M Human Chorionic Gonadotropin (hCG) is a 40 kD glycoprotein secreted in large quantities by the placenta and normally circulates at readily detectable levels only during gestation. Immunohistochemical studies reveal localization of hCG in syncytiotrophoblasts. Isolated clusters of giant cells may be found in association with certain components of germ cell tumors but are most frequently associated with embryonic carcinoma, endodermal sinus tumor, and germinoma. This antibody stains the cytoplasm of positive cells. IgA P P Clone: HSA/E8 Isotype: IgG1/K Source: Mouse Immunogen: Human HSA Specificity: HSA Localization: Cytoplasm Pre-treatment: EZ-AR™ 1 Manual/i6000: HK521-XAK AM550-5M Concentrated: MU550-UC Recommended Positive Control: FG-550M Polyclonal Source: Rabbit Immunogen: IgA isolated from human serum Specificity: IgA Localization: Membrane & Cytoplasm Pre-treatment: EZ-AR 1/EZ-AR 2 Xmatrx: HX031-YCDE/HX032-YCDE Tonsil tissue stained with anti-IgA using AEC chromogen Liver tissue stained with Anti-HSA using DAB as a chromogen Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Clone: Ready-to-use (Manual): AR045-5R Ready-to-use (Automated): Xmatrx® AW045-YCD Concentrated: PU045-UP IgA is the predominant antibody isotype in mucosal areas. This antibody reacts with IgA but not with other isotypes. It is useful in the evaluation of leukemias, plasmacytomas, certain non-Hodgkin’s lymphomas, and glomerulonephritis. AM550-10M AX550-YCD Hepatocyte Specific Antigen (HSA) has been demonstrated consistently in the vast majority of hepatocellular carcinomas. HSA recognizes both benign and malignant liver derived tissues including such tumors as hepatoblastoma, Hepatocellular carcinoma, and hepatic adenoma. It recognizes both normal adult and fetal liver tissue. This antibody is useful in differentiating hepatocellular carcinomas with adenoid features from adenocarcinomas, either primary in the liver or metastatic lesions to the liver. In recognizing hepatoblastoma, it is useful in differentiating this entity from other small round cell tumors. internationalcs@biogenex.com P - Tissue Type FFPE F - Tissue Type Frozen 103 Primary Antibodies IgD IgG P Polyclonal Clone: Polyclonal Source: Rabbit Source: Rabbit Immunogen: IgD isolated from a pool of normal human sera Immunogen: IgG isolated from human serum Specificity: Human IgD Specificity: IgG Localization: Membrane & Cytoplasm Localization: Membrane & Cytoplasm Pre-treatment: AR-10/EZ-AR 1/EZ-AR 2 Pre-treatment: EZ-AR 1/EZ-AR 2 Manual: HK058-5K Xmatrx: HX031-YCDE/HX032-YCDE Xmatrx: HX031-YCDE/HX032-YCDE Tonsil tissue stained with Anti-IgG using AEC chromogen Tonsil stained with Anti-IgD using AEC chromogen Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Concentrated: PU440-UP Recommended Positive Control: FG-440P AR440-5R Ready-to-use (Manual): AR050-5R AW440-YCD Ready-to-use (Automated): Xmatrx® AW050-YCD Concentrated: PU050-UP Recommended Positive Control: FG-050P IgD is expressed on mature B cells and may be used to classify B cell neoplasms. Mantle zone B-cells in primary follicles and those outlining the germinal centers of secondary follicles are seen to be positive for IgD expression. Thus, this antibody could be used to detect changes in nodal architecture. It also may be used to detect the expanded follicular structures of progressive transformation of germinal center (PTGC), which are composed largely of IgD+ mantle zone B-cells. It is used along with IgM as a marker to identify marginal zone lymphomas. IgG The human B-lymphocyte is characterized by the presence of readily detectable surface immunoglobulins. Up to 10 percent of peripheral blood lymphocytes and 68-70 percent of the lymphocytes in lymph nodes are of the B-cell type. The patterns of reactivity to IgG, IgA, IgM, C3, kappa, and lambda light chains can be used for the characterization of certain kinds of kidney and skin diseases. This antibody stains human IgG in cytoplasm and membrane of B-cells and is negative for light chains and other heavy chains. IgM P P Clone: IgG88 Clone: IgM88 Isotype: IgG1 Kappa Isotype: IgG 2b Kappa Source: Mouse Source: Mouse Immunogen: Purified human immunoglobulin Immunogen: Purified human IgM Specificity: IgM Specificity: IgG Localization: Membrane & Cytoplasm Localization: Membrane & Cytoplasm Pre-treatment: Pepsin Pre-treatment: EZ-AR 1/EZ-AR 2 Xmatrx: HX031-YCDE/HX032-YCDE Tonsil stained with Anti-IgG using AEC chromogen Ready-to-use (Manual): AM367-5M Ready-to-use (Automated): Xmatrx® Concentrated: AX367-YCD Recommended Positive Control: FG-367M MU367-UC The molecular weight of IgG is 150 kD consisting of two gamma heavy chains and two kappa or lambda light chains. Immunohistochemical techniques to identify immunoglobulins have been used in the classification of leukemias, plasmacytomas and certain non-Hodgkin’s lymphomas. In addition, immunoglobulin immunohistochemistry has been widely used in nephropathology and dermatopathology for studying a variety of immune diseases. 104 P Clone: P - Tissue Type FFPE F - Tissue Type Frozen Manual/Xmatrx: EK000-5K Tonsil stained with Anti-IgM using DAB chromogen Ready-to-use (Manual): AM366-5M Ready-to-use (Automated): Xmatrx® AX366-YCD Concentrated: MU366-UC Recommended Positive Control: FG-366M This monoclonal antibody reacts with human IgM heavy (mu) chain Fc region of 900kD pentameric IgM. It does not react with IgA, IgG or with light chains. This antibody stains plasma cells containing IgM, but does not usually stain immune complexes and surface IgM. It is useful for the evaluation of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas, the majority of which derive from B-cell lineage. The common underlying feature of these malignancies is the restricted expression of heavy and light chains to a single heavy and light chain type. www.biogenex.com Primary Antibodies IgM Insulin P IgM expression in tonsil stained using DAB chromogen Clone: HB125 Source: Rabbit Isotype: IgG 1, Kappa Immunogen: IgM isolated from human plasma Source: Mouse Purified human insulin Insulin IgM antigen Specificity: Localization: Membrane & Cytoplasm Localization: Cytoplasm Pre-treatment: AR Citra Plus /EZ-AR 1/ EZ-AR 2 Pre-treatment: EZ-AR 1 Xmatrx: HX031-YCDE Manual/i6000 HK081-5KE Xmatrx: HX031-YCDE/HX032-YCDE AR427-5R Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AR427-10R AW427-YCD PU427-UP Recommended Positive Control: FG-427P Recommended Barrier Control: FB-427P This polyclonal antibody reacts with mu-chains of human IgM. All B-cells have IgD and IgM expressed predominantly on the surface and presumably act as antigen receptors. Surface IgM is present on mantle zone and marginal zone B-cells. Immature B-cells in bone marrow express IgM and mature B-cells migrating to periphery secrete IgD and IgM. The demonstration of both IgM and IgD can be useful in determining if a B-cell lymphoma is derived from mantle or marginal zone. Pancreas tissue stained with AntiInsulin using AEC chromogen Ready-to-use (Manual): AM029-5M Ready-to-use (Automated): Xmatrx® Concentrated: AX029-YCD Recommended Positive Control: FG-029M Clone: R1 Isotype: IgG2a Source: Mouse Immunogen: Synthetic peptide from 1-32 peptide of the alpha subunit of human Inhibin alpha Specificity: Inhibin Alpha Localization: Cytoplasm Pre-treatment: AR Citra Plus/EZ-AR 1 Manual: HK081-5KE Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® AM446-10M AX446-YCD Concentrated: MU446-UC Recommended Positive Control: FG-446M Insulin P Insulin Pancreas stained with antiInsulin using DAB chromogen Clone: EP125 Isotype: IgG Source: Rabbit Immunogen: A synthetic peptide corresponding to residues in human Insulin protein Specificity: Human Insulin protein Localization: Cytoplasm Pre-treatment: None Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM446-5M Inhibins are dimeric gonadal protein hormones that negatively regulate pituitary FSH synthesis and secretion. Inhibin contains an alpha and beta subunit linked by disulfide bonds. Two forms of inhibin differ in their beta subunits (A or B), while their alpha subunits are identical. Inhibin B is comprised of the Inhibin alpha subunit disulfide linked to the Inhibin beta subunit. Initial studies indicated that Inhibin is a critical negative regulator of gonadal stromal cell proliferation and was the first secreted protein identified to have tumor-suppressor activity. Inhibin alpha-subunit immunoreactivity has been detected in Sertoli cells, spermatocytes, and in some Leydig cells. internationalcs@biogenex.com MU029-UC Lack of this hormone gives rise to diabetes mellitus. The development of specific antibodies to various polypeptide hormones have made IHC localization of these hormones such as Insulin (which is produced in the pancreas by beta cells of Islet of Langerhans) the most sensitive and reliable means available for an accurate characterization of the function of islet cell tumors. This antibody recognizes the A chain loop of human Insulin. Cross-reactivity with bovine, rat and mouse Insulin has been observed. This antibody stains insulin in cytoplasm of beta cells in pancreas. P Ovary tissue stained with Anti-Inhibin Alpha using DAB chromogen Immunogen: Specificity: Ready-to-use (Manual): Inhibin Alpha P Polyclonal Clone: AN735-5M Recommended Positive Control: AN735-10M AY735-YCD NU735-UC FG-735N Recommended Barrier Control: FB-735N Insulin is a hormone that regulates glucose homeostasis. It is synthesized in the pancreas within the β-cells of the islets of Langerhans. One million to three million islets of Langerhans (pancreatic islets) form the endocrine part of the pancreas, which is primarily an exocrine gland. The endocrine portion accounts for only 2% of the total mass of the pancreas. Within the islets of Langerhans, beta cells constitute 65–80% of all the cells. The antibody labels both normal and neoplastic insulin-producing cells. It is useful in identifying insulinoma. P - Tissue Type FFPE F - Tissue Type Frozen 105 Primary Antibodies J Chain Kappa Light Chain P JC88 Clone: K88 Isotype: IgG 1 Kappa Isotype: IgG1, Kappa Source: Mouse Source: Mouse Immunogen: Human J chain Immunogen: Human kappa protein Specificity: J chain Specificity: Kappa light chain Localization: Cytoplasm Localization: Cytoplasm Pre-treatment: EZ-AR 1 Pre-treatment: EZ-AR 1 Xmatrx HX031-YCDE Manual/i6000: None Xmatrx: HX031-YCDE Plasma cell in the tonsil showing Kappa light chain positivity stained using DAB chromogen Tonsil stained with Anti-J-chain using Fast Red chromogen Ready-to-use (Manual): AM374-5M Ready-to-use (Automated): Xmatrx® AX374-YCD Concentrated: MU374-UC J chain is a small, glycopeptide of 15 kD. It is structurally unrelated to heavy or light chains, but is synthesized by all plasma cells that secrete polymeric immunoglobulins. J chains are present in a large proportion of the immunoglobulin-positive cells in the germinal centers of the tonsils and lymph nodes. B cells secrete J chain at an early stage of differentiation with the expression persisting in those cells destined to produce IgA or IgM. Kappa Light Chain P Tonsil stained with Anti-Kappa Light Chain using AEC chromogen Clone: L1C1 Isotype: IgG1 Ready-to-use (Manual): AM369-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Recommended Positive Control: Recommended Barrier Control: AM369-10M AX369-YCD MU369-UC FG-369M FB-369M This antibody reacts specifically with the kappa light chain of human immunoglobulin and not lambda light chain and is reactive with intact IgG (kappa), IgM (kappa), free kappa light chains, and Bence-Jones kappa light chains. The most important use of this antibody would be in distinguishing atypical reactive follicular lymphoid hyperplasia from follicular lymphoma, undifferentiated carcinoma from large cell lymphoma, pseudolymphoma from lymphoma, and reactive plasmacytosis from well differentiated plasmacytoma. Ki-67 Source: Mouse Immunogen: B-lymphoma cells Clone: K-2 Specificity: Kappa light chain Isotype: IgG1 Kappa Localization: Cytoplasm Source: Mouse Pre-treatment: EZ-AR 1 Immunogen: Manual/i6000: None Recombinant Ki-67 protein fragment close to C-terminus Xmatrx: HX031-YCDE Specificity: Ki-67 antigen Ready-to-use (Manual): AM048-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM048-10M AX048-YCD Recommended Positive Control: FG-048M MU048-UC The light chains of immunoglobulin molecules have two antigenic types: kappa and lambda. A given immunoglobulin molecule contains two light chains, either both kappa or both lambda. As a result the clonal nature of any immunoglobulin-producing cell population can be determined by its light chain structure. The most important use of this technique would be in distinguishing atypical reactive follicular lymphoid hyperplasia from follicular lymphoma, undifferentiated carcinoma from large cell lymphoma, pseudolymphoma from lymphoma, and reactive plasmacytosis from well differentiated plasmacytoma. 106 P Clone: P - Tissue Type FFPE F - Tissue Type Frozen P Medulloblastoma stained with AntiKi-67 using DAB chromogen Localization: Nucleus Pre-treatment: AR Citra Plus/EZ-AR 2 Manual/i6000: HK081-5KE Xmatrx: HX032-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Recommended Positive Control: Recommended Barrier Control: AM410-5M AM410-10M AX410-YCD MU410-UC FG-410M FB-410M Ki-67 reacts with a human nuclear antigen that is expressed in proliferating cells but not in resting cells. Ki-67 antigen is a potent tool for rapidly evaluating the growth fraction of any given human cell subset. It is particularly useful in studying malignant tumors and other pathogenic states as a measure of the proportion of proliferating cells. Immunostaining of Ki-67 antigen in normal tissue shows nuclear reactivity in cells of germinal centers of cortical follicles, cortical thymocytes, neck cells of gastrointestinal mucosa, and undifferentiated spermatogonia. www.biogenex.com Primary Antibodies Ki-67 Ki-67 Antigen, Proliferating Cell P EPR3611 Clone: MIB-1 Isotype: IgG Isotype: IgG1 Source: Rabbit Source: Mouse Immunogen: A synthetic peptide corresponding to residues in human Ki-67. Immunogen: Peptide fragment of Ki-67 antigen Specificity: Ki-67 antigen Specificity: Ki-67 Localization: Nucleus Nucleus Pre-treatment: AR Citra/EZ-AR 2 Manual/i6000 HK087-5KE Xmatrx: HX032-YCDE Localization: Tonsil stained with Anti-KI67 using DAB chromogen P Clone: Pre-treatment: EZ-ARTM2 Manual/i6000: HK522-XAK Xmatrx: HX032-YCDE Ready-to-use (Manual): AN527-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Recommended Positive Control: Tonsil stained with Anti-KI67 using AEC chromogen Ready-to-use (Manual): AM297-5M AN527-10M AX527-YCD NU527-UC Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM297-10M AX297-YCD FG-527N Recommended Positive Control: FG-297M Ki-67 antigen is a nuclear antigen specifically associated with cell proliferation. Ki-67 is expressed in all proliferating cells which are in the active phases of the cell cycle (late G1, S, G2, and mitosis), but absent in resting cells. In G1, it is predominately localized in the perinucleolar region and also found in the nuclear matrix in the later phases. Ki67 is thought to be involved in the maintaining of cell proliferation; it is considered an important marker in human tumor diagnostics and prognostics. Ki-67 Ki-67 is one of the most widely studied proliferating cell antigens. The expression of Ki-67 antigen is limited to cells in phase G1, S and G2 with the highest levels present in the M phase. Ki-67 is more likely to be expressed in aneuploid tumors compared to diploid tumors, and it is associated with a high mitotic count and high histology grade.This monoclonal antibody enables detection of Ki-67 in proliferating cell populations in routine paraffin sections. The antibody stains positive in the nucleus of proliferation cells. Ki-67 Antigen, Proliferating Cell P Tonsil stained with anti-ki67 antibody using DAB chromogen MU297-UC P Clone: EP5 Clone: Ki88 Isotype: IgG Isotype: IgG1, Kappa Source: Rabbit Source: Mouse Immunogen: A synthetic peptide corresponding to residues in human Ki-67 protein Immunogen: Recombinant human Ki-67 protein Specificity: Ki-67 antigen Specificity: Ki-67 Localization: Nucleus Pre-treatment: EZ-AR™ 2 Manual/i6000: HK527-XAK Xmatrx None Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Lymph node germinal cells stained with Anti-Ki-67 using DAB chromogen AN727-5M Recommended Positive Control: AN727-10M AY727-YCD NU727-UC FG-727N Recommended Barrier Control: FB-727N Ki-67 antigen is a nuclear antigen specifically associated with cell proliferation. Ki-67 is expressed in all proliferating cells which are in the active phases of the cell cycle (late G1, S, G2, and mitosis), but is absent from resting cells (G0). It is strictly associated with cell proliferation. Ki-67 labeling index has been shown to be elevated in early stage and further increased in advanced stage of various types of cancer including breast cancer, colon cancer, prostate cancer and brain cancer. internationalcs@biogenex.com Localization: Nucleus Pre-treatment: AR Citra Plus/EZ-AR 2 Manual/i6000 HK081-5KE Xmatrx: HX032-YCDE Ready-to-use (Manual): AM370-5M Ready-to-use (Automated): i6000TM Xmatrx® AM370-10M AX370-YCD Concentrated: MU370-UC Recommended Positive Control: FG-370M Recommended Barrier Control: FB-370M The monoclonal antibody Ki88 reacts with a human nuclear antigen expressed in proliferating cells but absent in resting cells. Immunostaining of the Ki-67 the antigen in normal tissue shows nuclear reactivity in cells of germinal centers of cortical follicles, cortical thymocytes, neck cells of gastrointestinal mucosa, and undifferentiated spermatogonia. Resting cells such as lymphocytes, monocytes, parietal cells and Paneth’s cells of gastrointestinal mucosa, hepatocytes, renal cells, and mature sperm cells do not stain. This antibody stains a human nuclear antigen expressed in all proliferating cells. P - Tissue Type FFPE F - Tissue Type Frozen 107 Primary Antibodies Lambda Light Chain Laminin P P Clone: Polyclonal Clone: Polyclonal Source: Rabbit Source: Rabbit Immunogen: Pool of human lambda Bence Jones proteins Immunogen: Laminin isolated from EHS-mouse sarcoma Specificity: Lambda light chains Specificity: Laminin Localization: Cytoplasm Localization: Basement Membrane Pre-treatment: EZ-AR 1 Pre-treatment: Xmatrx: HX031-YCDE Pepsin, RT, 10 min. /EZAR 1/EZ-AR 2 Manual/i6000: EK000-5K Xmatrx: HX031-YCDE/HX032-YCDE Lung stained with Anti-laminin using DAB chromogen Tonsil stained with Anti-lambda light chain using DAB chromogen AR049-5R Ready-to-use (Manual): AR078-5R Ready-to-use (Automated): Xmatrx® AW049-YCD Concentrated: Recommended Positive Control: PU049-UP FG-049P Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AR078-10R AW078-YCD PU078-UP Recommended Positive Control: FG-078P Recommended Barrier Control: FB-078P Ready-to-use (Manual): The light chains of immunoglobulin molecules may be either Kappa or Lambda. Antibodies to kappa and lambda light chains are used for the evaluation of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas, the majority of which are derived from B-cell lineage. The most important uses of this technique would be in distinguishing atypical reactive follicular lymphoid hyperplasia from follicular lymphoma, undifferentiated carcinoma from large cell lymphoma, pseudolymphoma from lymphoma, and reactive plasmacytosis from well differentiated plasmacytoma. Lambda Light Chain P Clone: EP172 Isotype: IgG Source: Rabbit Clone: Polyclonal A recombinant protein fragment corresponding to human IgA protein Source: Rabbit Immunogen: Lysozyme isolated from the urine of monocytic leukemia patients Lysozyme Immunogen: Tonsil stained with anti-Lambda using DAB chromogen Lysozyme P Specificity: Human IgA protein Localization: Membrane/Cytoplasm Specificity: Pre-treatment: AR-10/EZ-AR 2 Localization: Cytoplasm Manual/i6000: HK058-5K Pre-treatment: EZ-AR 1 Xmatrx HX032-YCDE Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN715-5M Recommended Positive Control: AN715-10M AY715-YCD NU715-UC FG-715N Recommended Barrier Control: FB-715N The basic structure of an immunoglobulin molecule consists of two identical heavy chains, either γ, µ, α, δ or ε and two identical light chains, either kappa or lambda. The gene rearrangement process that generates the immunoglobulin molecule results in either a productive kappa or lambda gene. The ratio of kappa and lambda light chains varies between Ig classes and subclasses. The lambda light chain antibody labels the lambda light chain that expresses normal and neoplastic B lymphocytes and plasma cells. Other cells may also express lambda light chain due to nonspecific uptake of immunoglobulin. The occurrence of a mixture of kappa and lambda chain expressing cells suggests a polyclonal population and a reactive or non-neoplastic proliferation of B cells. 108 The antibody to laminin selectively and specifically recognizes basement membrane components. Laminin consists of a 220 kD subunit, which is disulfide-linked into larger complexes or even into the structural scaffolding of the basement membrane itself. In surgical pathology, laminin can be used as a marker to demonstrate morphologic change of basement membrane, which is helpful for interpreting the invasion of malignant tumors. Laminin could also be used to study histogenesis and pathogenesis of certain unknown lesions such as extracellular and intracellular hyaline bodies occurring in various diseases. This antibody stains Laminin in basement membranes. P - Tissue Type FFPE F - Tissue Type Frozen Lymph Node stained with antilysozyme using AEC chromogen Ready-to-use (Manual): AR024-5R Ready-to-use (Automated): i6000TM Xmatrx® AR024-10R AW024-YCD Concentrated: PU024-UP Recommended Positive Control: FG-024P Recommended Barrier Control: FB-024P Lysozyme (also called muramidase) is an enzyme which acts on bacterial cell walls by cleaving N-acetyl-glucosaminyl-N-acetylmuramic acid linkages. Lysozyme is present in human milk, tears, saliva, and serum. It is also found in myeloid cells, monocytes and histiocytes, making it useful for the demonstration of the myeloid or monocytic nature of acute leukemia. This antibody stains the cytoplasm of granulocytes and monocytes/macrophages. www.biogenex.com Primary Antibodies Macrophage Melan-A (MART-1) P Kupffer cells stained with antiMacrophage using DAB chromogen P Clone: LN5 Isotype: IgM Source: Mouse Immunogen: Human peripheral blood cells Specificity: Macrophages Localization: Cytoplasm Pre-treatment: EZ-AR 1 Xmatrx: HX031-YCDE Melanoma stained with Anti-Melan-A using DAB chromogen Clone: A103 Isotype: IgG Source: Mouse Immunogen: Recombinant Melan-A protein Specificity: Melan-A or MART-1 Localization: Cytoplasm Pre-treatment: AR Citra/EZ-AR 1 Manual/i6000: HK087-5KE Xmatrx: HX031-YCDE Ready-to-use (Manual): AM165-5M Ready-to-use (Manual): AM361-5M Ready-to-use (Automated): Xmatrx® Concentrated: AX165-YCD MU165-UC Recommended Positive Control: FG-165M Ready-to-use (Automated): i6000TM Xmatrx® AM361-10M AX361-YCD Concentrated: MU361-UC Recommended Barrier Control: FB-361M Recommended Positive Control: FG-361M LN5 stains an unidentified antigen in cytoplasm of macrophages and histiocytes in hematopoietic organs. It stains mantle zone B lymphocytes of the lymph node and spleen, spermatogonia, chief cells of the stomach, ductal epithelium of breast and tubular epithelium of kidney. It is strongly reactive with cases of true histiocytic lymphoma but is negative, except for macrophages, in Hodgkins disease and non-Hodgkins lymphomas. It can be an important tool for the study of malignant and benign histiocytic lesions. This antibody stains the cytoplasm of a specific population of human macrophage and histiocytes. Mast Cell Tryptase P Mast cell in the dermis highlighted by Mast Cell Tryptase antibody using AEC chromogen Clone: AA1 Isotype: IgG1 Source: Mouse Immunogen: Human Mast Cell Tryptase purified from human lung tissue Melan-A, a product of the MART-1 gene, is a differentiation antigen which is expressed in 100% of melanocytes, most melanomas, and 50-60% of melanoma cell lines. It is one of the melanoma antigens recognized by autologous cytotoxic T cells, and as an antigenic target for tumor infiltrating lymphocytes. This antibody also stains Melan-A in normal melanocytes and in the retina. It does not stain normal or tumor tissues from non-melanocyte lineages. This antibody stains positive in cytoplasm of melanocytes and other positive cells. Melanoma P Clone: HMB45 Isotype: IgG1 Source: Mouse Immunogen: Metastatic malignant melanoma cells Malignant melanoma Specificity: Mast Cell Tryptase antigen Specificity: Localization: Cytoplasm Localization: Cytoplasm Pre-treatment: EZ-AR 1 Pre-treatment: AR Citra Plus/EZ-AR 1 Manual/i6000: None Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Melanoma tumor cells positive for melanoma antigen stained using AEC chromogen AM419-5M Ready-to-use (Manual): AM001-5M AX419-YCD Ready-to-use (Automated): i6000TM Xmatrx® AM001-10M AX001-YCD AM419-10M MU419-UC Concentrated: Recommended Positive Control: MU001A-UC FG-419M Recommended Positive Control: Recommended Barrier Control: FG-001M FB-419M Recommended Barrier Control: FB-001M The monoclonal antibody produced by clone AA1 reacts with human Mast Cell Tryptase in different tissues. Relatively high levels of the enzyme are found in mast cells of skin and lung. Tryptase, a structurally unique trypsin like serine protease, is a biochemical marker that has proven useful for disorders that involve systemic mast cell activation. It is shown to be implicated as a potential mediator in the pathology of several mast cell related allergic and inflammatory conditions, including rhinitis, conjunctivitis, and most notably asthma. This antibody stains Mast Cell Tryptase antigen in cytoplasm of mast cells in skin, lung and tonsil tissues. internationalcs@biogenex.com Metastatic melanoma is often confused with a variety of poorly differentiated carcinomas, sarcomas, and large cell lymphomas. Clone HMB45 reacts with fetal and neonatal melanocytes but not with normal adult melanocytes and junctional nevus cells but not with intradermal nevi, hence showing specificity for detection of melanocytic tumors. The panel of tumor markers, most commonly used in conjunction with HMB45, for evaluation of melanoma includes S-100 protein LCA, CEA, and EMA, as well as vimentin, an intermediate filament found in both melanomas and sarcomas. P - Tissue Type FFPE F - Tissue Type Frozen 109 Primary Antibodies Melanoma gp100 Mesothelin P Melanoma tissue stained with Anti-Melanoma gp100 using AEC chromogen Clone: gp100/D5 Isotype: IgG1/K Source: Mouse Immunogen: Human melanoma gp100 Specificity: Melanoma gp100 Localization: Cytoplasm Pre-treatment: AR Citra Solution/EZ-AR 1 Manual/i6000: HK087-5KE Xmatrx: HX031-YCDE Ready-to-use (Manual): Ovary adenoma stained with antiMesothelin using DAB chromogen AM536-5M Ready-to-use (Automated): i6000TM Xmatrx® AM536-10M AX536-YCD Concentrated: MU536-UC Recommended Positive Control: FG-536M Melanoma gp100/D5 is a mouse monoclonal antibody that reacts against an antigen present in melanocytic tumors such as melanomas. It reacted positively against melanocytic tumors but not other tumors, thus demonstrating specificity and sensitivity. This antibody is very useful to identify malignant melanoma. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, sarcomas, spindle cell carcinomas and various types of mesenchymal neoplasms. Melanoma-Associated Antigen P Melanoma stained with AntiMelanoma associated antigen using DAB chromogen Clone: NKI/C3 Isotype: IgG1 Source: Mouse Immunogen: Purified membranes of human melanoma cells Clone: 5B2 Isotype: IgG1 Source: Mouse Immunogen: Prokaryotic recombinant fusion protein corresponding to approximately 100 amino acids from membrane bound form of mesothelin. Specificity: Mesothelin Localization: Membrane Pre-treatment: AR-10/EZ-AR 1 Manual/i6000: HK058-5K Xmatrx: HX031-YCDE Ready-to-use (Manual): AM433-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM433-10M AX433-YCD Recommended Positive Control: FG-433M MU433-UC Mesothelin, a 40kD glycosyl-phosphatidylinositol–linked cell surface glycoprotein, is present on the surface of the mesothelial cells and may be involved in cell adhesion. It is also seen on mesotheliomas, epithelial ovarian cancers, and some squamous cell carcinomas. Clone 5B2 reactivity has been seen in epitheloid mesotheliomas and adenocarcinomas of lung, ovary, peritoneum, endometrium, pancreas, stomach and colon to a varying degree. Mesothelin is abundant in normal mesothelial cells from which malignant mesotheliomas and ovarian cystadeno carcinomas are derived. This antibody can be used in conjunction with an antibody to calretinin for evaluation of mesotheliomas. MiTF P Clone: MiTF/A13 Isotype: IgG1/k Mouse Specificity: NKI/C3 antigen Source: Localization: Membrane & Cytoplasm Immunogen: Human MiTF Pre-treatment: EZ-AR 1/EZ-AR 2 Specificity: MiTF Manual/i6000: None Localization: Nuclear Pre-treatment: EZ-ARTM 2 Manual/i6000: HK522-XAK Xmatrx: HX032-YCDE Xmatrx: HX031-YCD/EHX032-YCDE Ready-to-use (Manual): AM077-5M Ready-to-use (Automated): i6000TM Xmatrx® AM077-10M AX077-YCD Concentrated: MU077-UC Recommended Positive Control: Recommended Barrier Control: FG-077M FB-077M The melanoma associated antigen is a formalin resistant glycoprotein with a disulphide dependent configuration that is essential for recognition by the NKI/C3 monoclonal antibody. This antibody recognizes a heterogeneous 25-110 kD glycoprotein that is located mainly in the inner side of membranes of cytoplasmic vesicles in melanoma cells. This antibody reacts with melanoma, nevocellular nevi, carcinoids and medullary carcinomas of the thyroid. It does not react with basal cell carcinoma, brain tissue or brain tumors. 110 P P - Tissue Type FFPE F - Tissue Type Frozen Melanoma tissue stained with AntiMiTF using AEC chromogen Ready-to-use (Manual): AM554-5M Ready-to-use (Automated): i6000TM Xmatrx® AM554-10M AX554-YCD Concentrated: MU554-UC Recommended Positive Control: FG-554M Micropthalmia-associated Transcription Factor (MiTF) is a basic helixloop-helix leucine zipper transcription factor involved in melanocyte and osteoclast development. Mutations in MiTF cause auditory pigmentary syndromes, such as Waardenburg Syndrome Type II, Type IIa and Tietz Syndrome in humans. MiTF plays a critical role in the differentiation of various cell types such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. This antibody recognizes serine phosphorylated and non-phosphorylated melanocytic isoforms of micropthalmia. It is useful in identifying malignant melanoma, and distinguishing mast cell lesions of myeloid derivation. A relatively rare class of tumors known as PEComas (tumors showing perivascular epitheloid cell differentiation) express MiTF in a high percentage of cases ~90%). www.biogenex.com Primary Antibodies Mitochondrial Antigen P MMP-9 P Clone: 113-1 Clone: EP1255Y Isotype: IgG1 Isotype: IgG Source: Mouse Source: Rabbit Immunogen: Raji Burkitt's lymphoma cells Immunogen: A synthetic peptide corresponding to residues in the C-terminus of Human MMP-9. MMP-9 Specificity: Mitochondria Localization: Cytoplasm Pre-treatment: AR Citra Specificity: Manual: HK087-5KE Localization: Cytoplasm Pre-treatment: EZ-AR™ 2/EZ-AR 1 Manual/i6000: HK522-XAK Lymphoma tissue stained with AntiMMP-9 using DAB chromogen Fetal Liver tissue stained with Anti-Mitochondrial Ag using AEC chromogen Concentrated: Xmatrx: MU213-UC Monoclonal antibody 113-1 recognizes a 60 kD antigen of human mitochondria. This marker may be useful in identification of mitochondria in cells, tissues, and biochemical preparations. It produces a “spaghetti-like” staining pattern in the cytoplasm of human cells and may be used as a marker of biliary cirrhosis. The antibody stains mitochondria in cytoplasm of positive cells. P Human colon stained with AntiMLH1 using DAB chroogen ES05 Isotype: IgG1 Source: Mouse Immunogen: MLH1 Specificity: MLH1 Localization: Nuclei Pre-treatment: AR Citra Plus/EZ-AR 1 Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN504-10M AX504-YCD NU504-UC Recommended Positive Control: FG-504N MSH2 P AM703-5M Clone: SP46 Isotype: IgG Source: Rabbit Immunogen: Synthetic peptide corresponding to internal region of human MSH2 FG-703M Specificity: Human MSH2 FB-703M Localization: Nucleus Pre-treatment: EZ-AR™ 1 Manual/i6000: HK521-XAK Ready-to-use (Automated): i6000TM Xmatrx® AM703-10M AY703-YCD Concentrated: MU703-UC Recommended Positive Control: Recommended Barrier Control: MLH1 is a mismatch repair protein involved in maintaining the integrity of genetic information alongside MSH2. MSH6 and PMS2. During DNA replication, strand misalignment can occur resulting in alterations to microsatellite repeats, often referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been linked to human carcinogenesis. Mutations in the MLH1 gene have been reported to be found in tumors with MSI, such as some forms of colon cancer e.g., Hereditary nonpolyposis colon cancer (HNPCC), a subset of sporadic carcinomas and breast cancer. internationalcs@biogenex.com AN504-5M Type IV collagenase (MMP-9), a 92-kD member of matrix metalloproteinase, is believed to play a critical role in the physiological tissue-remodeling processes and also in many pathological conditions such as tumor invasion. The induction of proteolytic enzymes is an important mechanism in the migration of monocytes into tissues and body fluids. MMP-9 is secreted from neutrophils, macrophages, and a number of transformed cells in zymogen form. MMP-9/gelatinase B is upregulated in angiogenic dysplasias and invasive cancers of the epidermis in a mouse model of multi-stage tumorigenesis elicited by HPV16 oncogenes. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. Mismatch Protein Repair (MLH1) Clone: HX031-YCDE Ready-to-use (Manual): Colon Ca. stained with anti-MSH2 using DAB chromogen Xmatrx: Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: None AN743-5M AN743-10M AY743-YCD NU743-UC MutS homologue 2 (MSH2) is a DNA mismatch repair protein that belongs to the MutS family. MSH2 forms two different heterodimers: MutS alpha (MSH2-MSH6) and MutS beta (MSH2-MSH3), which bind to DNA mismatches thereby initiating DNA repair. MSH2 is involved in DNA repair as a mismatch repair protein, and mutations of MSH2 are found in approximately 50% of inherited nonpolyposis colorectal carcinoma (HNPCC) (Lynch syndrome) cases. P - Tissue Type FFPE F - Tissue Type Frozen 111 Primary Antibodies MSH6 MUC5AC P Colon carcinoma stained with AntiMSH6 using DAB chromogen 2D4B5 Clone: 45M1 Isotype: IgG3 Isotype: IgG1 Source: Mouse Source: Mouse Immunogen: Human MSH6 Immunogen: Human MUC5AC Specificity: MSH 6 Specificity: MUC5AC Localization: Nucleus Localization: Cytoplasm Pre-treatment: EZ-ARTM 2/EZ-AR 1 Pre-treatment: EZ-ARTM 1 Manual/i6000: HK521-XAK Xmatrx: HX032-YCDE AM454-5M Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM454-10M AX454-YCD MU454-UC Recommended Positive Control: FG-454M Mouse anti-MSH6 is a monoclonal antibody specific for MSH6. Inherited (germline) mutations in DNA mismatch repair genes such as MLH1, MSH2, MSH3, and MSH6 are the major causes of hereditary nonpolyposis colorectal cancer (HNPCC) syndrome. A characteristic of HNPCC tumors is microsatellite instability (MSI). Detection of microsatellite instability in a tumor sample will increase the probability of detecting a germline mutation in a DNA mismatch repair gene from the patient sample. Thus, MSI analysis is usually performed prior to proceeding with full mutation analysis of mismatch repair genes. Gastro-intestinal tissue stained with Anti-MUC5AC using DAB chromogen Colonic mucosa stained with MUC4 antibody showing diffuse cytoplasmic positivity. (DAB chromogen used) 1G8 Isotype: IgG1 Source: Mouse Immunogen: Human MUC4 Specificity: MUC4 HX031-YCDE Ready-to-use (Automated): i6000TM Xmatrx® AM456-10M AX456-YCD Concentrated: MU456-UC Recommended Positive Control: FG-456M Mucins are high molecular weight glycoproteins with 80% carbohydrates and 20% core protein. Gastric Mucin 5AC antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden Mucin 5AC antigen. P Clone: EPR1023 Isotype: IgG Source: Rabbit Immunogen: A synthetic peptide corresponding to residues on the C-terminus in human MUC1 Mucin 1 Localization: Cytoplasm Specificity: Pre-treatment: EZ-ARTM 1/EZ-AR 2 Localization: Cytoplasm Manual/i6000: HK521-XAK Pre-treatment: AR Citra Plus/EZ-AR 1 Xmatrx: HX031-YCDE/HX032-YCDE Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE Ready-to-use (Manual): AM455-5M Ready-to-use (Automated): i6000TM Xmatrx® AM455-10M AX455-YCD Concentrated: MU455-UC Recommended Positive Control: FG-455M MUC4 is a membrane-associated protein of the mucin (MUC) gene family, encoded by a gene on chromosome 3q29 and produced by epithelial cells as a heterodimer. The MUC4 protein is thought to play a protective role for vulnerable epithelia, particularly in the airway, eye, female reproductive tract, and mammary gland. Alterations in MUC4 expression have been observed in association with a variety of inflammatory and neoplastic states; reduction or loss has been reported in non-small cell lung carcinoma, hyperplastic polyps of the colon, and serrated colon adenomas, while overexpression of the MUC4/Sialomucin complex (SMC) has been identified in malignant progression of mammary tumors in humans. 112 HK521-XAK Xmatrx: AM456-5M P Clone: Manual/i6000: Ready-to-use (Manual): Mucin 1 (MUC1) MUC4 P Clone: P - Tissue Type FFPE F - Tissue Type Frozen Breast carcinoma stained with AntiMucin-1 using DAB chromogen Ready-to-use (Manual): AN564-5M Ready-to-use (Automated): i6000TM Xmatrx® AN564-10M AX564-YCD Concentrated: NU564-UC Recommended Positive Control: FG-564N Mucin 1 (MUC1) is a high molecular weight and heavily glycosylated transmembrane protein. MUC1 is normally expressed in secretory epithelial cells and functions to provide a protective layer and mediate cell-cell interactions. MUC1 is a prominent oncogene and is present in many carcinomas with abnormal expression levels, modified glycosylation, and aberrant intracellular localization. Such carcinomas include breast, colorectal, prostate, and pancreatic cancer. www.biogenex.com Primary Antibodies Mucin 2 (MUC2) Muscle Actins P Colon stained with Anti-Mucin 2 using DAB chromogen P Clone: CCP58 Clone: Actin 88 Cocktail Isotype: IgG1 Isotype: IgG Source: Mouse Source: Mouse Immunogen: Synthetic human MUC2 (MI-29) peptide (VNTR region) Immunogen: Synthetic peptides of actin Specificity: MUC2 Specificity: Muscle actins Localization: Cytoplasm Pre-treatment: AR-10/EZ-AR 1 Manual HK058-5K Xmatrx: HX031-YCDE Ready-to-use (Manual): AM358-5M Ready-to-use (Automated): Xmatrx® AX358-YCD Concentrated: MU358-UC Muscle tissue stained with AntiActin, Muscle Specific, using DAB chromogen Localization: Cytoplasm Pre-treatment: EZ-AR1 Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Concentrated: Mucins are a group of high molecular weight, highly glycosylated proteins expressed in normal and carcinogenic colon. MUC2 is a 520kD glycoprotein of the gastrointestinal tract. The core of the glycoprotein consists of a variable number of tandem repeats of a 23 amino acid sequence. Mucin 2 is found in normal epithelial cells of the colon or in colon carcinoma. MUC2 glycoprotein is expressed in mucinous tumors but not in serous tumors. This antibody stains positive for colon gastric cancer cells, normal intestine, colon and salivary glands, and some human colon carcinoma cell lines (LS174T). This antibody localizes Mucin 2 (MUC2) protein in cytoplasm. AM381-5M AX381-YCD MU381-UC Actin is a major component of the cytoskeleton and is present in every cell type. It is a globular protein, about 5 nm in diameter, composed of one polypeptide chain with a mass of approximately 47 kD. Four muscle actins have been identified: skeletal alpha, cardiac alpha, vascular smooth muscle alpha, and enteric smooth muscle gamma actin. These actins are very similar in their primary structure. Monoclonal Actin 88 is for the specific localization of actins in muscle tissue. Staining with this antibody distinguishes smooth muscle cells from fibroblasts in mixed cultures. This antibody stains skeletal, cardiac and smooth muscle cells. Myelin Basic Protein P Multi-Drug Resistance Marker (P-Glycoprotein) P Adrenal gland tissue stained with Anti-multi-drug resistance marker using AEC chromogen Clone: MDR88 Isotype: IgG1 Kappa Source: Mouse Immunogen: Recombinant P-glycoprotein containing four tandem repeats of the amino acid sequence 1096 through 1252, once of the cytoplasmic domains near the C-terminus Specificity: Multi-Drug Resistance Marker Localization: Membrane & Cytoplasm Pre-treatment: AR Citra/EZ-AR 1/EZ-AR 2 Manual: HK087-5KE Xmatrx: HX031-YCD/EHX032-YCDE Ready-to-use (Manual): AM391-5M Ready-to-use (Automated): Xmatrx® AX391-YCD Concentrated: MU391-UC Multi-Drug Resistance Marker (P-Glycoprotein) is a 170 kD cell membrane protein of the multi-drug resistance gene, MDR-1. Studies have linked the presence of P-Glycoprotein with resistance to a wide variety of chemotherapeutic agents. P-Glycoprotein is associated with an afflux pump that actively removes drug from the cell, thereby conferring resistance to a variety of drugs. P-Glycoprotein is also found in various concentrations in most normal tissues, suggesting that the primary role for this protein is in normal secretion of physiological metabolites. This antibody stains P-Glycoprotein in membrane and certain degree of cytoplasm of positive cells. internationalcs@biogenex.com Cerebellum tissue stained with Anti-Myelin basic protein using AEC chromogen Clone: MBP88 Isotype: IgG1 Source: Mouse Immunogen: This antibody is the fusion product of SP/2 myeloma cells and the splenocytes from an A/J mouse immunized with peptide of Myelin Basic Protein Specificity: Myelin Basic Protein Localization: Cytoplasm Pre-treatment: EZ-AR 1 Manual/i6000: None Xmatrx: Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® HX031-YCDE AM380-5M AM380-10M AX380-YCD Myelin Basic Protein (MBP), a single-chain, flexible polypeptide of about 18.5 kD is localized in both the compact myelin sheath and myelin ovoids. MBP has not been demonstrated in rough endoplasmic reticulum, lysosomes, or any other cytoplasmic organelles. MBP can be used as a marker for oligodendrocytes, Schwann cells and malignant Schwannomas. This antibody is useful in defining some of the elements in the catabolism of myelin in multiple sclerosis, experimental encephalomyelitis, and other diseases of the central nervous system. This antibody stains Myelin Basic Protein. P - Tissue Type FFPE F - Tissue Type Frozen 113 Primary Antibodies Myeloperoxidase (MPO) Myeloid Specific Antigen P P Bone marrow stained with AntiMyeloid Specific Antigen using Fast Red chromogen Clone: BM-3 Clone: Polyclonal Isotype: IgG1 Isotype: IgG Source: Mouse Source: Rabbit Immunogen: Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes Immunogen: Purified human granulocytic MPO Specificity: Myloperoxidase Specificity: Myeloid Specific Antigen Localization: Cytoplasm Pre-treatment: AR-10/EZ-AR 1 Manual: HK058-5K Xmatrx: HX031-YCDE Ready-to-use (Manual): AM216-5M Ready-to-use (Automated): Xmatrx® AX216-YCD BM-3 is an early marker of myeloid differentiation. BM-3 recognizes a 13 kD myeloid specific antigen. The BM-3 antibody along with BM-1 and BM-2, provides the capacity to stain early precursor and mature forms of human myeloid cells. It is expressed during the early phases of myeloid differentiation. This antigen is present in human granulocytes, monocytes, and myeloid precursor cells. It has no reactivity with any other cell type in human tissues. This antibody stains cytoplasm in human granulocytes (98%) and monocytes (80%) residing in lymphoid and non-lymphoid tissues in formalin-fixed, paraffin-embedded tissue sections, bone marrow smears or blood smears. Myeloid Specific Antigen Spleen tissue stained with AntiMyeloperoxidase using DAB chromogen Localization: Cytoplasm Pre-treatment: EZ-AR™ 1/EZ-AR™ 2 Manual/i6000: HK522-XAK Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AR496-5R Recommended Positive Control: FG-496P AR496-10R AW496-YCD PU496-UP Myeloperoxidase is an important enzyme used by granulocytes during phagocytic lysis of foreign particles engulfed. In normal tissues and in a variety of myeloproliferative disorders, myeloid cells of both neutrophilic and eosinophilic types at all stages of maturation, exhibit strong cytoplasmic reactivity for MPO. Erythroid precursors, megakaryocytes, lymphoid cells, mast cells, and plasma cells are nonreactive. MPO is not observed in the neoplastic cells of a wide variety of epithelial tumors and sarcomas. MPO is useful in differentiating between myeloid and lymphoid leukemias. P Lymph node stained with AntiMyeloid Specific Antigen using AEC chromogen Clone: BM-1 Isotype: IgG1 Source: Mouse Immunogen: Nuclei from human peripheral blood mononuclear cells Specificity: Myeloid Specific Antigen Localization: Cytoplasm Pre-treatment: EZ-AR 2 Xmatrx: HX032-YCDE P Rhabdomyosarcoma stained with Anti-Myf4 using DAB chromogen Ready-to-use (Manual): AM164-5M Ready-to-use (Automated): Xmatrx® AX164-YCD Concentrated: MU164-UC Recommended Positive Control: FG-164M This 183 kD myeloid specific antigen is a DNA binding protein expressed in early precursor myeloid cells. Monoclonal antibodies BM-1 and BM-2 are useful in the identification of early precursor and mature forms of human myeloid cells, respectively. The antigens are also expressed in granulocytic sarcomas and myeloid leukemias, myeloid precursor cells of bone marrow, scattered cells in the peripheral cortex of the thymus, granulocytes, granulocytic sarcomas, acute myelogenous leukemia (AML), chronic myelogenous leukemias and myelomonocytic leukemias. This antibody stains myeloid specific antigen in the nucleus of myeloid precursor cells of bone marrow, scattered cells in the peripheral cortex of the thymus, granulocytes, and granulocytic sarcomas. 114 Myf4 P - Tissue Type FFPE F - Tissue Type Frozen Clone: LO26 Isotype: IgG1 Source: Mouse Immunogen: Recombinant fusion protein corresponding to the Myf4 gene Specificity: Myf4 Localization: Nucleus Pre-treatment: EZ-AR 2 Manual: HK522-XAK Xmatrx: HX032-YCDE Ready-to-use (Manual): AM432-5M Ready-to-use (Automated): i6000TM Xmatrx® AM432-10M Concentrated: MU432-UC Recommended Positive Control: FG-432M AX432-YCD Myf4 belongs to the family of the muscle regulatory proteins Myf3, Myf4, Myf5, and Myf6 that share a highly conserved DNA binding and dimerization domain consisting of a cluster of basic amino acids and a potential helix-loop-helix structure. Myogenin (Myf4) expressed early in skeletal muscle differentiation is a sensitive and specific marker for rhabdomyosarcoma and is more specific than desmin and musclespecific actin and more sensitive than myoglobin. www.biogenex.com Primary Antibodies Myoglobin Myosin Heavy Chains, Smooth Muscle P Skeletal Muscle stained with AntiMyoglobin using DAB chromogen Clone: MG-1 Isotype: IgG1 P SMMS.1 Isotype: IgG1 Source: Mouse Source: Mouse Immunogen: Purified human skeletal muscle myoglobin Immunogen: Crude human uterus caldesmon Specificity: Myoglobin Specificity: Localization: Cytoplasm Smooth muscle myosin heavy chains Pre-treatment: EZ-AR 1 Localization: Cytoplasm Xmatrx: HX031-YCDE Pre-treatment: AR-10/EZ-AR 2 Myoepithelial cells stained with Anti-Myosin heavy chains using AEC chromogen Ready-to-use (Manual): AM012-5M Ready-to-use (Automated): Xmatrx® Concentrated: AX012-YCD Recommended Positive Control: FG-012M MU012-UC Myoglobin functions as a cellular oxygen storage mechanism with some contribution to oxygen transport into the cell. The molecular mass of human myoglobin is 17.8 kD. Myoglobin is present exclusively in striated muscle, with the single exception of chicken gizzard smooth muscle. It is a valuable tool used in distinguishing rhabdomyosarcomas from other soft tissue tumors. After muscle tissue damage such as crush injuries, burns, myocardial infarction and muscle diseases, increased levels of myoglobin are found in the blood and urine. This antibody stains positive in the cytoplasm of muscle cells. Myoglobin Clone: P Clone: Polyclonal Source: Rabbit Immunogen: Highly purified human myoglobin Manual/i6000: HK058-5K Xmatrx: HX032-YCDE Ready-to-use (Manual): AM331-5M Ready-to-use (Automated): i6000TM Xmatrx® AM331-10M AX331-YCD Concentrated: MU331-UC SMMS.1 is approximately 204 kD and is considered to be the marker for smooth muscle cell phenotypes. It has been designed for specific localization of both vascular and visceral smooth muscle. Monoclonal antibody to smooth muscle myosin heavy chains in combination with monoclonal antibodies to calponin and heavy caldesmon may be used to study the differences between benign, in-situ lesions and invasive carcinomas. Monoclonal antibody stains smooth muscle myosin heavy chains in vascular and visceral smooth muscle, myoepithelial cells in normal and benign human mammary gland and certain stromal myofibroblasts. Myosin, Skeletal Muscle P Clone: MY-32 IgG1 Mouse Specificity: Myoglobin Isotype: Localization: Cytoplasm Source: Pre-treatment: EZ-AR 1 Immunogen: Rabbit muscle myosin Xmatrx: HX031-YCDE Specificity: Skeletal-muscle myosin Skeletal muscle stained with AntiMyoglobin using AEC chromogen Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Concentrated: Recommended Positive Control: AR012-5R Cytoplasm Pre-treatment: EZ-AR 1/EZ-AR 2 Xmatrx: HX031-YCDE/HX032-YCDE Skeletal muscle stained with AntiMyosin using DAB chromogen AW012-YCD PU012-UP FG-012P Myoglobin functions as a cellular oxygen storage mechanism with some contribution to oxygen transport into the cell. The molecular mass of human myoglobin is 17.8 kD. Myoglobin is present exclusively in striated muscle, with the single exception of chicken gizzard smooth muscle. Since myoglobin is the only striated muscle-specific antigen, it is a valuable tool used in distinguishing rhabdomyosarcomas from other soft tissue tumors. After muscle tissue damage such as crush injuries, burns, myocardial infarction and muscle diseases, increased levels of myoglobin are found in the blood and urine. This antibody reacts with human myoglobin. internationalcs@biogenex.com Localization: Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Concentrated: AM109-5M AX109-YCD MU109-UC Recommended Positive Control: FG-109M Myosin along with actin forms the fundamental contractile unit of muscle, the myofibril. It has a molecular mass of 500 kD and is comprised of two identical heavy chains (200 kD each) and four light chains (15-20 kD). Monoclonal antibody MY-32 to fast-twitch skeletal myosin may be used for detecting cross-striated muscle differentiation in tumors. This antibody does not stain human or animal cardiac or smooth-muscle myosin. Staining of fast-twitch (type II) isomyosin molecules has been demonstrated on human skeletal muscle. The antibody stains human, rabbit, rat, mouse, bovine, chicken, and guinea pig skeletal myosin. P - Tissue Type FFPE F - Tissue Type Frozen 115 Primary Antibodies Napsin A Neurofilament P Lung adenocarcinoma stained with anti-Napsin A using DAB chromogen Clone: IP64 Isotype: IgG2b Source: Mouse Immunogen: Napsin IgG1 Source: Mouse Immunogen: Neurofilament purified from human brain Neurofilaments Napsin A Specificity: Cytoplasm Localization: Cytoplasm Pre-treatment: EZ-AR™ 1 Pre-treatment: Npne Manual/i6000: HK521-XAK Xmatrx: HX031-YCDE Ready-to-use (Automated): i6000TM Xmatrx® AM701-10M AY701-YCD Concentrated: MU701-UC Recommended Positive Control: FG-701M Recommended Barrier Control: FB-701M Napsin A has specific funtion in normal alveolar epithelim and is proposed to play a role in the protelytic processing of surfactant precursors. Napsin A is reported to be predominantly expressed in lamellar bodies of type II pneumocutes, secondary lysosymes of alveolar macrophages, respiratory epithelium of terminal and respiratory bronchioles, plasma cells within a subset of lymphocytes in normal lung, as well as in epithellial cells of renal tubiles in normal kidney and is weakly expressed in normal spleen. Nerve stained with AntiNeurofilament using AEC chromogen Ready-to-use (Manual): AM073-5M Ready-to-use (Automated): i6000TM Xmatrx® AM073-10M AX073-YCD Concentrated: MU073-UC Recommended Positive Control: FG-073M Recommended Barrier Control: FB-073M Neurofilaments (10 nm diameter) and microtubules (25 nm diameter) comprise the main structural elements of neuronal axons, dendrites, and perikerya. Neurofilaments are composed of three major polypeptides referred to as the neurofilament triplet with approximate molecular weights of 200 kD, 160 kD and 68 kD. This antibody can be used for positive identification of neurons in the central and peripheral nervous systems. In general, co-expression of keratin and neurofilament should be interpreted as indicating neuroendocrine differentiation of a given tissue or neoplasm. The antibody stains Neurofilament in sections of brain and other tissues. Neuron Specific Enolase (NSE) P Clone: EPR1792Y Isotype: IgG Source: Rabbit Immunogen: A synthetic peptide corresponding to residues on the C-terminus of human Cadherin-2. Specificity: N-Cadherin Localization: Membrane Pre-treatment: AR Citra Plus /EZ-AR 1/ EZ-AR 2 Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): AN565-5M Ready-to-use (Automated): i6000TM Xmatrx® AN565-10M AX565-YCD Concentrated: NU565-UC Recommended Positive Control: FG-565N N-Cadherin (Cadherin 2) is a calcium-dependent transmembrane protein involved in cell-cell adhesion. N-cadherin functions during gastrulation to regulate mesenchymal morphogenesis and angiogenesis. N-Cadherin has been noted for its role in cancer progression, particularly tumor metastasis. In the progression of a tumor, metastasis is often facilitated by the upregulation of N-Cadherin. The aberrant expression of N-Cadherin allows transendothelial migration which is needed for metastasis to occur while the lack of N-Cadherin will inhibit it. 116 NE-14 Isotype: Localization: AM701-5M Colon carcinoma stained with Anti-N-Cadherin using DAB as chromogen Clone: Specificity: Ready-to-use (Manual): N-Cadherin P P - Tissue Type FFPE F - Tissue Type Frozen P Pancreatic islets stained with antiNSE using DAB chromogen Clone: MIG-N3 Isotype: IgG1 Kappa Source: Mouse Immunogen: Purified human gamma enolase Specificity: Neuron specific enolase (NSE) Localization: Cytoplasm Pre-treatment: None Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM055-5M AM055-10M AX055-YCD MU055-UC Recommended Positive Control: FG-055M Recommended Barrier Control: FB-055M NSE is a gene which encodes for a protein found in matured neurons and is used in panels along with chromogranin, synaptophysin and neurofilament. Elevated NSE concentrations are observed in patients with neuroblastoma, pancreatic islet cell carcinoma, medullary thyroid carcinoma, pheochromocytoma, and other neuroendocrine tumors as well as certain benign conditions. NSE is specific for such proteins, and aids in detection of neural and neuroendocrine lineage. www.biogenex.com Primary Antibodies NGF Receptor Nuclear Ribonucleoprotein P Neuroblastoma stained with Anti-NGF Receptor using DAB chromogen Clone: EP1039Y Isotype: IgG Source: Rabbit Immunogen: A synthetic peptide corresponding to residues of human NGFR P 58-15 Isotype: IgG Source: Mouse Immunogen: Isolated nuclei Specificity: Nuclear Ribonucleo protein particles Specificity: NGF Receptor Localization: Nucleus Localization: Membrane Pre-treatment: Pepsin Pre-treatment: EZ-ARTM 2/EZ-AR 1 Manual: EK000-5K Manual/i6000: HK522-XAK Xmatrx: HX031-YCDE Ready-to-use (Manual): Spleen tissue stained with AntiNuclear ribonucleoprotein using DAB chromogen AN522-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN522-10M AX522-YCD NU522-UC Neurotrophins signal cell survival, differentiation, growth cessation, and apoptosis through two classes of cell surface receptors, the Trks and NGF Receptor (p75NTR). p75NTR, when activated in the absence of a strong Trk signal, induces apoptosis of neurons, while in the presence of Trk it enhances responses to neurotrophin. p75NTR, when it is not bound by NGF, may play a role in neuronal selection during embryonic development and suggest that neuroblastomas may arise from immature neuroblasts that escape programmed cell death. Therefore, the loss of p75NTR expression in developing neural crest cells might be a primary event in the genesis of neuroblastoma. NGF Receptor Clone: Concentrated: MU230-UC Recommended Positive Control: FG-230M Monoclonal antibody 58-15 is one such antibody that recognizes a 36 kD nuclear antigen that is organized into discrete 20-80 nm electrondense nuclear granules. Immunohistochemical analyses in a variety of different tumors indicate that antinuclear monoclonal antibodies may be useful for probing the cell cycle dependent modulation of nuclear antigens. This antibody stains a 20-80 nm electron dense nuclear structure with highest labeling densities found in nuclear ribonucleoprotein particles, although heterochromatin, euchromatin, and nucleoli may also be stained. Oct-2 P Clone: EPR542 Isotype: IgG Source: Rabbit Immunogen: A synthetic peptide corresponding to residues in human Oct.-2 Specificity: Oct-2 P Brain stained with anti-NGFR using DAB chromogen Clone: EP31 Isotype: IgG Source: Rabbit Immunogen: A synthetic peptide corresponding to residues of human NGFR protein Specificity: NGFR Localization: Cytoplasm Pre-treatment: AR Citra Plus/EZ-AR 1 Manual/i6000: HK081-5KE Xmatrx HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN738-5M Recommended Positive Control: AN738-10M AY738-YCD NU738-UC FG-738N Recommended Barrier Control: FB-738N Tonsil stained with Anti-OCT-2 using DAB chromogen Localization: Nuclear Pre-treatment: AR Citra Plus/EZ-AR 2 Manual/i6000: HK081-5K Xmatrx: HX032-YCDE Ready-to-use (Manual): AN558-5M Ready-to-use (Automated): i6000TM Xmatrx® AN558-10M AX558-YCD Concentrated: NU558-UC Recommended Positive Control: FG-558N Oct-2 is a transcription factor belonging to the POU homeo-domain family and is a major regulator found in B lymphocytes. By binding to the octamer motif, Oct-2 promotes immunoglobulin transcription and regulates various lymphoid-specific genes. All tumor cell lines from the B-cell lineage, and a few from the T-cell lineage, express Oct-2. Oct-2 expression is significantly greater in diffuse large B-cell lymphoma and lymphocyte predominant Hodgkin disease; therefore Oct-2 is a useful differential marker for these diseases. NGFR, also known as p75NTR, is a receptor of neurotrophins and involved in survival, differentiation and apoptosis of neurons. It is expressed in neuronal cells in various tissues and tumors with neuronal origin. NGFR is also expressed in melanocytes, myoepithelial cells, basal-like cells, perivascular cells and lymphoid dendritic cells. NGFR is helpful in identification of perineural invasion of malignant skin tumors with a panel of antibodies. It is also a complementary marker to S-100 for identification of desmoplastic melanomas. internationalcs@biogenex.com P - Tissue Type FFPE F - Tissue Type Frozen 117 Primary Antibodies Oct-4 p105 Proliferation-Associated Nuclear Antigen P Testis stained with anti-Oct-4 using DAB chromogen Clone: EP143 Isotype: IgG Source: Rabbit Immunogen: A synthetic peptide corresponding to residues of human Oct-4 protein Specificity: Oct-4 Localization: Nucleus Pre-treatment: AR Citra Plus/EZ-AR 2 Manual/i6000: HK081-5KE Xmatrx: HX032-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Recommended Positive Control: Recommended Barrier Control: FB-724N Oct-4 transcription factor is initially active as a maternal factor in the oocyte but remains active in embryos throughout the preimplantation period. Oct-4 expression is associated with an undifferentiated phenotype and tumors. Oct-4 is a sensitive and specific marker for germ cell tumors. It is consistently detected in carcinoma in situ/ gonadoblastoma, seminomas, germinoma, dysgerminoma, and embryonal carcinoma but not in the differentiated components of nonseminomas, i.e., teratomas, yolk sac tumors, and choriocarcinomas. It is useful in the identification of primary as well as metastatic germ cell tumors. Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Concentrated: Clone: OST1 Isotype: IgG1 2B3 Isotype: IgM Source: Mouse Immunogen: Pokeweed mitogenstimulated human peripheral blood lymphocytes Specificity: p105 proliferationassociated nuclear antigen Localization: Nucleus Pre-treatment: Pepsin/EZ-AR 2 Manual: EK000-5K Xmatrx: HX032-YCDE AM317-5M Ready-to-use (Automated): Xmatrx® AX317-YCD Concentrated: MU317-UC Antibody to p105 is directed against two polypeptides with molecular mass of 105 and 41 kD. Anti-p105 is the only immunological reagent known to preferentially stain interchromatin granules, a domain within the nuclear matrix responsible for RNA synthesis. The p105 antigen is thought to play an important role in RNA metabolism, RNA transport, or cell-cycle regulation. Like Ki-67, p105 is a proliferation-associated nuclear antigen that shows increased expression in proliferating cells. In normal cells p105 staining is absent during the early phases of the cell cycle. During G2 and mitosis, p105 levels increase dramatically. Antibody to p105 may prove useful for identifying malignancies and in studying chromatin structure and malignant transformations. P Clone: G175-405 Isotype: IgG Source: Mouse Immunogen: Human p16 (INK4a) fusion protein Source: Mouse Immunogen: Human osteonectin Specificity: Osteonectin protein Localization: Cytoplasm Specificity: P16 Pre-treatment: AR Citra/EZ-AR 1 Localization: Manual/i6000: HK087-5KE Nucleus and/or Cytoplasm Xmatrx HX031-YCDE Pre-treatment: AR Citra Plus Manual/i6000: HK081-5KE Cervical carcinoma stained with Anti-p16 using DAB as chromogen AM387-5M AX387-YCD MU387-UC Bone matrix consists of collagen and non-collagenous proteins. Osteonectin, a 32-kD calcium-binding glycoprotein, is found in a variety of cell types, which include osteoblastic epithelial cells and fibroblasts. Osteonectin is a useful biochemical marker for bone-related tumors. Thus, osteonectin antibody can be used to demonstrate the presence of osteonectin in active osteoblasts and osteoprogenitor cells as well as in young osteocytes. 118 Clone: Ready-to-use (Manual): p16 (INK4a) P Osteosarcoma stained with AntiOsteonectin using DAB chromogen Oral mucosa stained with Anti-p105 using AEC chromogen AN724-5M AN724-10M AY724-YCD NU724-UC FG-724N Osteonectin P P - Tissue Type FFPE F - Tissue Type Frozen Ready-to-use (Manual): AM540-5M Ready-to-use (Automated): i6000TM Concentrated: AM540-10M Recommended Positive Control: FG-540M MU540-UC RU O p16(INK4a) is a tumor-suppressor protein and that genetic and epigenetic abnormalities in genes controlling the G1 checkpoint can lead to both escape from senescence and cancer formation. The interaction of p16(INK4a) family members can be a binary complex with CDK4/6 or ternary complex with cyclin D-bound CDK4/6 and ultimately results in the inhibition of cell cycle progression. As such, expression of p16(INK4a) is commonly associated with cellular senescence, and disruption of the p16(INK4a) gene is frequently observed in human tumors. The p16(INK4a) locus is deleted in a wide spectrum of tumors including melanoma, pancreatic adenocarcinoma, glioblastoma, certain leukemias and non-small cell lung cancer. For research use only. Not for use in diagnostic procedures. www.biogenex.com Primary Antibodies p21/WAF1 P Skin stained with Anti-WAF-1using AEC chromogen p27 (Kip1) P Clone: 4D10 Clone: Y236 Isotype: IgG1 Isotype: IgG Source: Mouse Source: Rabbit Immunogen: Recombinant fusion protein corresponding to full length WAF1 molecule Immunogen: A synthetic peptide corresponding to residues in the C-term of human p27(Kip1). p27 Specificity: p21/WAF1 antigen Localization: Nucleus Specificity: Pre-treatment: AR Citra Plus/EZ-AR 2 Localization: Nucleus Manual: HK081-5KE Pre-treatment: EZ-ARTM 1/EZ-AR 2 Xmatrx: HX032-YCDE Manual/i6000: HK521-XAK Xmatrx: HX032-YCDE Ready-to-use (Manual): AM434-5M Ready-to-use (Automated): Xmatrx® Concentrated: AX434-YCD Recommended Positive Control: FG-434M Breast carcinoma stained with Anti-p27 Using DAB chromogen MU434-UC The p21/WAF1 protein is a p53 regulated gene product that has been shown to mediate cell cycle arrest. The growth arrest is due to several properties of this protein, namely cyclin dependent kinase inhibition, and maintenance of cell cycle arrest at G2 by blocking the interaction of Cdc25C with PCNA and inhibition of stress activated protein kinases. In breast cancer the p21/WAF1 expression is generally seen to be negative. This antibody stains the nucleus in cells that are arrested in G1 phase. p27 (Kip1) AN492-5M Ready-to-use (Automated): i6000TM Xmatrx® AN492-10M AX492-YCD Concentrated: Recommended Positive Control: NU492-UC FG-492N p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-CDK2 complex, cyclinA-CDK2 and cyclinD1-CDK4. p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm. p34cdc2 (Cyclin Dependent Kinase) P Breast tissue stained with Anti-p27/ Kip1 using AEC chromogen Ready-to-use (Manual): Clone: DCS72 Isotype: IgG1 Clone: POH-1 Source: Mouse Isotype: IgG2a Immunogen: Recombinant rodent p27/ Kip1 antigen Source: Mouse Immunogen: Recombinant human p34cdc2 fusion protein Specificity: p34cdc2 cyclin dependent kinase P Specificity: p27 Kip1 antigen Localization: Nucleus Pre-treatment: AR Citra Plus/EZ-AR 2 Manual: HK081-5KE Localization: Nucleus & Cytoplasm HX032-YCDE Pre-treatment: AR Citra /EZ-AR 1/ EZ-AR 2 Xmatrx: Tonsil tissue stained with Antip34cdc2 using DAB chromogen Manual: HK087-5KE Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): AM396-5M Ready-to-use (Automated): Xmatrx® AX396-YCD Ready-to-use (Manual): AM301-5M MU396-UC Ready-to-use (Automated): Xmatrx® Concentrated: AX301-YCD Recommended Positive Control: FG-301M Concentrated: The p27 Kip1 protein, also known as cyclin-dependent kinase inhibitor 1b (CDKN1B) or Kip1, is a putative tumor suppressor gene, regulator of drug resistance in solid tumors, and promoter of apoptosis. It acts as a safeguard against inflammatory injury and it has a role in cell differentiation. The p27 Kip1 protein is expressed in all normal tissues. The level of its expression has been observed to decrease during tumor development and progression in many tumors, including oral squamous cell carcinoma and in thyroid, colon, breast, prostate, and superficial bladder carcinomas. Overexpression of p27 Kip1 has been observed in a subset of aggressive B cell lymphomas. internationalcs@biogenex.com MU301-UC p34cdc2 is a phosphoprotein with protein kinase activity that functions in the G2/M phase transition of the cell cycle. It is the catalytic subunit of the maturation-promoting factor (MPF) and forms a complex with both cyclin A and B in mammalian cells. Activated p34cdc2 kinase phosphorylates a variety of substrates leading to some specific events of mitosis including nuclear envelope break-down and chromosome condensation. It has also been implicated in lymphoid proliferation. This antibody stains p34cdc2 cyclin dependent kinase in nucleus and cytoplasm of proliferating cells and tumor cells and cross-reacts with skeletal muscle cells. P - Tissue Type FFPE F - Tissue Type Frozen 119 Primary Antibodies P504S (AMACR) p53 P Prostate carcinoma stained with Anti-P504S antibody using DAB chromogen 13H4 Clone: EP9 Isotype: IgG Isotype: IgG Source: Rabbit Source: Rabbit Immunogen: Human AMACR polypeptide Immunogen: A synthetic peptide corresponding to N-terminal residues of human p53 protein Human p53 protein Specificity: P504S Localization: Cytoplasm Specificity: Pre-treatment: AR Citra Plus/EZ-AR 1 Localization: Nucleus Manual/i6000: HK081-5KE Pre-treatment: EZ-AR™ 2 Xmatrx: HX031-YCDE Manual/i6000: HK522-XAK Xmatrx: None Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN449-5ME Recommended Positive Control: FG-449N AN449-10ME AX449-YCDE NU449-UCE AMACR (P504S) is an acronym for the protein alpha-methylacyl CoA racemase that helps to metabolize certain fatty acids within the body. AMACR has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. High expression of AMACR (P504S) protein is usually found in prostatic adenocarcinoma but not in benign prostatic tissue by immunohistochemical staining in paraffin-embedded tissues. It stains premalignant lesions of prostate: high grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. Several studies have suggested that AMACR can be used as a prostate cancer biomarker. Using AMACR (P504S) as a positive marker along with basal cell staining (34 beta E12 or p63) as a negative marker could help to confirm the diagnosis of small focus of prostate carcinoma on needle biopsies. P504S (AMACR) Breast Ca. stained with anti-P53 Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Clone: RBT-AMACR Isotype: IgG Source: Rabbit Immunogen: Human P504S Specificity: P504S/AMACR Localization: Cytoplasm Pre-treatment: EZ-AR™ 1 Manual/i6000: HK521-XAK Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Recommended Positive Control: AN538-5M AN538-10M AX538-YCD NU538-UC FG-538N AMACR has been recently described as a prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. High expression of AMACR (P504S) protein is usually found in prostatic adenocarcinoma but not in benign prostatic tissue by immunohistochemical staining in paraffin-embedded tissues. It stains premalignant lesions of prostate: high grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. Using AMACR (P504S) as a positive marker along with basal cell staining (34 beta E12 or p63) as a negative marker could help to confirm the diagnosis of small focus of prostate carcinoma on needle biopsies. P - Tissue Type FFPE F - Tissue Type Frozen AN728-5M Recommended Positive Control: AN728-10M AY728-YCD NU728-UC FG-728N Recommended Barrier Control: FB-728N Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycles, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, and it functions as a tumor suppressor within a variety of tumors by either stimulating apoptosis or growth arrest in deference to cell type and physiological factors. p53 is overexpressed in over 50% of human cancers. Positive staining of p53 detected by immunohistochemistry has been observed in colon cancer, breast cancer, lung cancer, prostate cancer and ovary cancer. p53 Protein P P Prostate carcinoma stained with Rabbit Anti- P504S / AMACR using DAB as chromogen 120 P Clone: Breast carcinoma stained with Anti-p53 using DAB chromogen Clone: BP53-12-1 Isotype: IgG2a Source: Mouse Immunogen: Recombinant human wild-type p53 protein Specificity: p53 protein Localization: Nucleus Pre-treatment: AR Citra/EZ-AR 2 Manual/i6000: HK087-5KE Xmatrx: HX032-YCDE Ready-to-use (Manual): AM195-5M Ready-to-use (Automated): i6000TM Xmatrx® AM195-10M AX195-YCD Concentrated: MU195-UC Recommended Positive Control: FG-195M Recommended Barrier Control: FB-195M p53 is a tumor suppressor gene product identified in a wide variety of tumors. p53 protein is present in low concentration in normal cells, but elevated levels of mutant p53 have been found in many common cancers. Accumulation of mutant p53 detected by immunohistochemical staining has been reported in breast, lung, colon, stomach, bladder, and testis carcinomas, soft-tissue sarcomas, and melanomas. This antibody stains positive in nucleus of a variety of tumor cells. www.biogenex.com Primary Antibodies p53 Protein p63 P Breast carcinoma stained with Anti-p53 using DAB chromogen Clone: DO7 Isotype: IgG2b Source: Mouse Immunogen: Recombinant wild-type p53 protein Specificity: p53 protein Localization: Nucleus Pre-treatment: AR Citra/EZ-AR 2 Manual/i6000: HK087-5KE Xmatrx: HX032-YCDE Ready-to-use (Manual): AM239-5M Ready-to-use (Automated): i6000TM Xmatrx® AM239-10M AX239-YCD Concentrated: MU239-UC Recommended Positive Control: FG-239M Recommended Barrier Control: FB-239M Prostate tissue stained with Anti-p63 using AEC chromogen Breast carcinoma stained with Anti-p53 using DAB chromogen 1801 Isotype: IgG1 Mouse Immunogen: Fusion proteins of human p53 with β-galactosidase p53 protein Localization: Nucleus Pre-treatment: AR Citra/EZ-AR 2 Manual/i6000: HK087-5KE Xmatrx: HX032-YCDE IgG2a Source: Mouse Immunogen: Amino terminal fragment of the delta Np63 isoform Specificity: p63 Localization: Nucleus Pre-treatment: AR Citra Plus/ EZ-AR 2 Manual/i6000TM: HK081-5K Xmatrx: HX032-YCDE AM418-10M AX418-YCD MU418-UC Recommended Positive Control: FG-418M PAP AM418-5M P Prostate Carcinoma stained with Anti-PAP using DAB chromogen Source: Specificity: 4A4 Isotype: Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: P Clone: Clone: Recommended Barrier Control: FB-418M This antibody will detect all isoforms of p63 since the epitope is within the DNA binding domain. The p63 protein is a member of the p53 family, which also includes p73. p63 protein is detected in proliferating cells of epithelium, cervix, urothelium and prostate. p53 is a tumor suppressor gene product identified in a wide variety of tumors. p53 protein is present in low concentration in normal cells, but elevated levels of mutant p53 have been found in many common cancers. Accumulation of mutant p53 detected by immunohistochemical staining has been reported in breast, lung, colon, stomach, bladder, and testis carcinomas, soft-tissue sarcomas, and melanomas. This antibody stains positive in nucleus of a variety of tumor cells. p53 Protein P Clone: A40010 Isotype: IgG1 Source: Mouse Immunogen: PAP purified from seminal fluid Specificity: PAP Localization: Cytoplasm Pre-treatment: EZ-AR™ 1 Manual/i6000 HK521-XAK Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM532-5M Recommended Positive Control: FG-532M AM532-10M AX532-YCD MU532-UC Prostate acid phosphatase (PAP) is a 100 kD glycoprotein present in high concentration in the prostate gland and its secretions. PAP is measured clinically because its level often rises in the serum in cases of prostatic carcinoma. Ready-to-use (Manual): AM240-5M Ready-to-use (Automated): i6000TM Xmatrx® AM240-10M AX240-YCD Concentrated: Clone: Cam Vir-1 MU240-UC Isotype: IgG 2a Recommended Positive Control: FG-240M Source: Mouse Immunogen: Recombinant HPV-16 protein Specificity: HPV16 Papillomavirus Type 16 (HPV-16) P p53 is a tumor suppressor gene product identified in a wide variety of tumors. p53 protein is present in low concentrations in normal cells, but elevated levels of mutant p53 have been found in many common cancers. Accumulation of mutant p53 detected by immunohistochemical staining has been reported in breast, lung, colon, stomach, bladder, and testis carcinomas, soft-tissue sarcomas, and melanomas. This antibody stains both wild-type and mutant human p53 protein primarily in nucleus of positive cells. HPV infected tissue stained with Anti-HPV 16 using DAB chromogen Localization: Nucleus Pre-treatment: AR Citra/EZ-AR 2 Manual/i6000: HK087-5KE Xmatrx: HX032-YCDE Ready-to-use (Manual): AM362-5ME Ready-to-use (Automated): Xmatrx® Concentrated: AX362-YCDE MU362-UCE Infection with certain types of HPV, the so-called “oncogenic HPVs” including 16, 18, 31, 33, and 35, has been associated as a major risk factor in the subsequent development of cervical cancer. internationalcs@biogenex.com P - Tissue Type FFPE F - Tissue Type Frozen 121 Primary Antibodies PDCD4 PAX-5 P B cell showing PAX-5 positivity in a reactive lymph node stained using DAB chromogen ZP007 Clone: EPR3432 Isotype: IgG1 Isotype: IgG Source: Mouse Source: Rabbit Immunogen: Human PAX-5 Immunogen: Specificity: PAX-5 antigen Localization: Nucleus Pre-treatment: EZ-ARTM 1/EZ-AR 2 A synthetic peptide corresponding to residues near the Cterminus of human PDCD4 . Manual/i6000: HK521-XAK Specificity: PDCD4 Xmatrx: HX032-YCDE Ready-to-use (Manual): AM457-5M Ready-to-use (Automated): i6000TM Xmatrx® AM457-10M AX457-YCD Concentrated: MU457-UC Recommended Positive Control: FG-457M The PAX-5 gene is a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. The PAX-5 gene encodes the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX-5 gene product may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis. Breast carcinoma stained with AntiPDCD4 using DAB chromogen Localization: Cytoplasm/Nucleus Pre-treatment: EZ-ARTM 1/EZ-AR 2 Manual/i6000: HK521-XAK Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): AN524-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN524-10M AX524-YCD NU524-UC Recommended Positive Control: FG-524N Programmed cell death protein 4 (PDCD4) is a tumor suppressor that inhibits tumor promoter-induced neoplastic transformation. It downregulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUNdependent transcription. PDCD4 inhibits the translation initiation factor eIF4A, an RNA helicase that catalyzes the unwinding of secondary structure at the 5’ untranslated region (5’UTR) of messenger RNAs (mRNAs). It is also believed that regulated degradation of PDCD4 in response to mitogens allows efficient protein synthesis and consequently cell growth. PDGF Receptor-β Paxillin (N-Term) P P Clone: Breast carcinoma stained with AntiPaxillin using DAB chromogen Y113 Isotype: IgG Source: Rabbit Clone: Y92 Isotype: IgG Source: Rabbit A synthetic peptide corresponding to residues near the N-terminus of human Paxillin Immunogen: Human- PDGFR- Beta Specificity: PDGFR- Beta Specificity: Paxillin Manual/i6000: HK521-XAK Localization: Cytoplasm Xmatrx: HX031-YCDE Pre-treatment: EZ-ARTM 1/EZ-AR 2 Manual/i6000: HK521-XAK Immunogen: Xmatrx: Ready-to-use (Manual): HX031-YCDE/HX032-YCDE AN521-5M Ready-to-use (Automated): i6000TM Xmatrx® AN521-10M AX521-YCD Concentrated: NU521-UC Recommended Positive Control: FG-521N Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Paxillin plays a significant role in integrin signaling and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization. Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118. It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration. 122 P Clone: P - Tissue Type FFPE F - Tissue Type Frozen GIST showing cytoplasmic PDGF expression stained using DAB chromogen Localization: Membrane Pre-treatment: EZ-ARTM 1 Ready-to-use (Manual): AN463-5M Ready-to-use (Automated): i6000TM Xmatrx® AN463-10M AX463-YCD Concentrated: NU463-UC Recommended Positive Control: FG-463N Human platelet-derived growth factor (PDGF) receptor, is a receptor tyrosine kinase whose activity induces various signalling cascades leading to cell growth and proliferation. Two major isoforms exist, PDGF receptor-α and PDGF receptor-β. PDGF receptor-β can only bind two specific PDGF isoforms, PDGF-β and PDGF-Δ. Upon ligand binding, the receptor undergoes autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as PI kinase, Grb2, and Src, among others. www.biogenex.com Primary Antibodies PGP9.5 Placental Lactogen (hPL) P Small intestine stained with antiPGP9.5 Clone: 3D9 Isotype: IgG2a kappa P Source: Mouse Immunogen: PGP9.5 antibody was raised in mouse using recombinant human PGP9.5 (1-223aa) purified from E. coli as the immunogen Specificity: Human PGP9.5 Localization: Cytoplasm Pre-treatment: AR Citra Plus//EZ-AR 1 Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM736-5ME Recommended Positive Control: FG-736M Recommended Barrier Control: FB-736M AM736-10ME AY736-YCDE MU736-UCE PGP9.5/UCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. PGP9.5 is a component of the ubiquitin system, which has value as a marker for neurons and may be of particular use in the study of ubiquitinated cellular inclusions characteristic of several chronic human neurodegenerative diseases. A fundamental role in regulating various biological activities, the PGP9.5 gene encodes two opposing enzymatic activities that affect alphasynuclein degradation and Parkinson’s disease susceptibility. Placenta tissue stained with Anti-Hpl using DAB chromogen Clone: Polyclonal Source: Rabbit Immunogen: Human placental lactogen purified from human urine Specificity: Human Placental Lactogen (hPL) Localization: Cytoplasm Pre-treatment: EZ-AR 1 Manual/i6000: None Xmatrx: AR040-5R AR040-10R AW040-YCD Concentrated: PU040-UP Recommended Positive Control: FG-040P Human Placental Lactogen (hPL) can be demonstrated in human placental tissue and in the serum of pregnant women. Human placental lactogen has been identified in some breast carcinomas and in trophoblastic and nontrophoblastic tumors of the placenta, and has been used as a serum or tissue marker for trophoblastic and nontrophoblastic neoplasms. This antibody stains hPL in cytoplasm of trophoblast and other positive cells. Platelet-Derived Growth Factor (PDGF) P Clone: PDGF88 Isotype: IgM Source: Mouse Immunogen: Synthetic peptide of PDGF-B conjugated to keyhole limpet hemocyanin PDGF-B Placental Alkaline Phosphatase (PLAP) P HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Clone: PL8-F6 Specificity: Isotype: IgG Localization: Cytoplasm Source: Mouse Pre-treatment: AR Citra/EZ-AR 1 Immunogen: Purified human placental alkaline phosphatase Specificity: Placental alkaline phosphatase Localization: Cytoplasm Pre-treatment: None Placenta tissue stained with antiPLAP using DAB chromogen Ready-to-use (Manual): AM228-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM228-10M AX228-YCD MU228-UC Recommended Positive Control: FG-228M Recommended Barrier Control: FB-228M Squamous cell carcinoma stained with Anti-PDGF using AEC chromogen Manual: HK087-5KE Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® AM376-5M Concentrated: MU376-UC AX376-YCD PDGF is one of the major factors activated in wound healing and revascularization and may play an important role as an endogenous promoter in epithelial tumor foundation. PDGF can lead to excessive production of extracellular matrix components including various collagens, proteoglycans, and laminin. The development of specific subsets of smooth muscle cells depends on PDGF. PDGF is one of the most potent activators of stromal cells. PDGFR is a prime candidate to mediate proliferation and migration responses of mesangial injury in glomerular disease. This monoclonal antibody stains PDGF in cytoplasm of positive cells. Human Placental Alkaline Phosphatase (PLAP), a 60-70 kD oncofetal antigen, is a member of a family of membrane bound alkaline phosphatase enzymes and isoenzymes. PLAP and/or PLAP-like isoenzymes have been found to be expressed by malignant tumors of germ cell and non-germ cell origin. The antibody reacts with PLAP in syncytiotrophoblasts in placenta and also reacts with human germ cell tumors. This antibody stains positive in cytoplasmic membrane and cytoplasm of positive cells. internationalcs@biogenex.com P - Tissue Type FFPE F - Tissue Type Frozen 123 Primary Antibodies Platelet-Derived Growth Factor (PDGF) P P Polyclonal Clone: EP2 Source: Rabbit Isotype: IgG Immunogen: Synthetic peptide based on PDGF-B sequence that shares high homology with PDGF-A forms Source: Rabbit Immunogen: Purified human progesterone receptor protein PDGF Specificity: Progesterone Receptor Cytoplasm Localization: Nucleus Pre-treatment: EZ-AR 1 Pre-treatment: AR Citra/EZ-AR 1 Xmatrx: HX031-YCDE Manual/i6000: HK087-5KE Xmatrx: HX031-YCDE Specificity: Localization: Squamous cell carcinoma stained with Anti-PDGF using AEC chromogen Progesterone Receptor Clone: Ready-to-use (Manual): AR376-5R Ready-to-use (Automated): Xmatrx® AW376-YCD Concentrated: PU376-UP PDGF is one of the major factors activated in wound healing and may play an important role as an endogenous promoter in epithelial tumor foundation. PDGF can lead to excessive production of extracellular matrix components including various collagens, proteoglycans, and laminin. PDGF is one of the most potent activators of stromal cells. Proliferation and migration are important responses of mesangial cell injury. PDGFR is a prime candidate to mediate these responses in glomerular disease. PDGF and PDGFR are upregulated in the mesangium during glomerular injury. The monoclonal antibody to PDGF-B has been studied for its potential clinical utility in wound healing and revascularization. This epitope-specific antibody stains PDGF in cytoplasm of positive cells. Breast carcinoma stained with AntiPR using DAB chromogen Ready-to-use (Manual): AN711-5ME Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Recommended Positive Control: AN711-10ME AY711-YCDE NU711-UCE FG-711N Recommended Barrier Control: FB-711N The human progesterone receptor (PR), is a ligand-activated transcription factor and is a member of the steroid receptor family. PR exists in human as two isoforms; PR-A (94 kD) which lacks the first 164 amino acids of PR-B and PR-B(114 kD).This anti-PR recognizes both PR-A and B. It labels epithelial cells of breast, ovary and endometrium. This antibody stains human progesterone receptor in tissue sections by immunohistochemical techniques Progesterone Receptor P PMS2 P Colon carcinoma stained with AntiPMS2 using DAB as chromogen Clone: EPR3947 Isotype: IgG Source: Rabbit Immunogen: A synthetic peptide corresponding to residues in human PMS2 . Specificity: PMS2 Localization: Nucleus Pre-treatment: AR Citra Plus /EZ-AR 2 Manual/i6000: HK081-5KE Xmatrx: HX032-YCDE Ready-to-use (Manual): AN577-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN577-10M AX577-YCD NU577-UC Recommended Positive Control: FG-577N PMS2 (post meiotic segregation increased 2) is a DNA mismatch repair protein that forms a heterodimer with MLH1. Mutations in these genes are associated with hereditary nonpolyposis colon cancer (HNPCC). It is demonstrated that some of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability, this may suggest that DNA mismatch repair is not only restricted to HNPCC tumors but is a common feature in tumor initiation or progression. 124 P - Tissue Type FFPE F - Tissue Type Frozen Breast carcinoma stained with AntiPR using DAB chromogen Clone: 1A6 Isotype: IgG1 Source: Mouse Immunogen: Synthetic peptide of progesterone receptor Specificity: Progesterone Receptor Localization: Nucleus Pre-treatment: AR Citra/EZ-AR 2 Manual/i6000: HK087-5KE Xmatrx: HX032-YCDE AM172-2ME Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® AN172-10ME AX172-YCDE Concentrated: Recommended Positive Control: MU172-UCE FG-172M The presence of progesterone receptor in human breast cancer has been proposed as a mechanism whereby tumor cells respond to estrogen, and its presence may therefore serve as a marker for enhanced hormone responsiveness. Historically, estrogen receptorpositive/progesterone receptor-positive breast carcinoma patients have demonstrated a better response to endocrine therapy than estrogen receptor-positive/ progesterone receptor-negative patients. The use of monoclonal antibodies to determine progesterone receptor status increases the predictive value of immunohistochemical analysis with respect to the response of human tumors to hormonal modulation. This antibody stains human nuclear progesterone receptor in tissue sections by immunohistochemical techniques. www.biogenex.com Primary Antibodies Progesterone Receptor (InSite® PR) Proliferating Cell Nuclear Antigen (PCNA) P Progesterone Receptor on breast carcinoma showing strong nuclear positivity using AEC chromogen P Clone: PR88 Clone: PC10 Isotype: IgG1 Kappa Isotype: IgG2a Source: Mouse Source: Mouse Immunogen: Purified human progesterone receptor protein Immunogen: Rat PCNA synthesized with the protein A expression vector pR1T2T Specificity: Progesterone Receptor Specificity: PCNA Localization: Nucleus Localization: Nucleus Pre-treatment: AR Citra/EZ-AR 2 Pre-treatment: AR Citra/EZ-AR 2 Manual/i6000: HK087-5KE Manual/i6000 HK087-5KE Xmatrx: HX032-YCDE Xmatrx: HX032-YCDE Ready-to-use (Manual): AM328-5ME Ready-to-use (Automated): i6000TM Xmatrx® AM328-10ME AX328-YCDE Concentrated: MU328-UCE Recommended Positive Control: FG-328M Recommended Barrier Control: FB-328M PCNA positivity in Breast carcinoma stained using DAB chromogen The use of monoclonal antibodies to determine Progesterone Receptor status increases the predictive value of immunohistochemical analysis with respect to the response of human tumors to hormonal modulation. Historically, estrogen receptorpositive/progesterone receptor-positive breast carcinoma patients have demonstrated a better response to endocrine therapy than estrogen receptor-positive/ progesterone receptor-negative patients. This antibody stains positive in nucleus of the receptor positive cells. Prolactin Ready-to-use (Manual): AM252-5M Ready-to-use (Automated): i6000TM Xmatrx® AM252-10M AX252-YCD Concentrated: MU252-UC Recommended Positive Control: Recommended Barrier Control: FG-252M FB-252M PCNA, also known as cyclin, is a 36 kD nonhistone nuclear protein that plays a fundamental role in the initiation of cell proliferation. PCNA is a cell cycle-regulated protein that preferentially occurs in dividing cells and is undetectable or present in small amounts in resting cells. Immunoperoxidase staining for PCNA in benign tissues has revealed positive nuclear staining in normal colonic crypt epithelium, gastric glandular cells, germinal center cells of lymph node, basal cells of skin, and renal tubular epithelial cells. The monoclonal antibody to PCNA might be an acceptable alternative to Ki-67 labeling in routinely processed tissues. This antibody stains PCNA in the nucleus of proliferating cells. P Prostate Specific Acid Phosphatase (PSAP) Clone: ME.121 Isotype: IgG1 Source: Mouse Clone: B01-94-21M-NA Immunogen: Human Prolactin Isotype: IgG1 Kappa Specificity: Prolactin Source: Mouse Localization: Cytoplasm/Membrane Immunogen: Pre-treatment: None Partially purified prostate acid phosphatase from human seminal plasma Specificity: Prostate Specific Acid Phosphatase (PSAP) P Pituitary gland stained with AntiProlactin using DAB chromogen Ready-to-use (Manual): AM031-5M Ready-to-use (Automated): i6000TM Xmatrx® AM031-10M AX031-YCD Concentrated: MU031-UC Prostate tissue stained with AntiPSAP using DAB chromogen Malignant pituitary adenomas or pituitary carcinomas arise from and consist of adenohypophysial cells. They can produce various hormones such as ACTH, Growth hormone, TSH, FSH, LH and Prolactin. Tumors that do not consist of adenohypophysial cells neither produce nor contain pituitary hormone, and thus immuno-peroxidase techniques are helpful in distinguishing from those pituitary tumors that store various hormones in the cell cytoplasm. internationalcs@biogenex.com Localization: Cytoplasm Pre-treatment: None Ready-to-use (Manual): AM013-5ME Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Recommended Positive Control: Recommended Barrier Control: AM013-10ME AX013-YCDE MU013-UCE FG-013M FB-013M RU O Prostate specific acid phosphatase (PSAP) is a 100 kD glycoprotein present in high concentration in the prostate gland and its secretions. PSAP is measured clinically because its level often rises in the serum in cases of prostatic carcinoma. By immunohistochemical analysis PSAP has been found concentrated within the large secretory vacuoles of the supranuclear portion of the prostatic columnar epithelial cell. In hyperplastic prostates and in benign prostatic tissue adjacent to the prostatic carcinoma, PSAP activity is limited to the acinar or ductal columnar epithelial cells and adjacent luminal content. For research use only. Not for use in diagnostic procedures. P - Tissue Type FFPE F - Tissue Type Frozen 125 Primary Antibodies Prostate Specific Antigen (PSA) PSMA P Clone: EP192 Isotype: IgG1 Isotype: IgG Source: Mouse Source: Rabbit Immunogen: A synthetic peptide corresponding to residues of human PSMA protein Specificity: PSMA protein Immunogen: Affinity purified prostate specific antigen Specificity: Prostate specific antigen (PSA) Localization: Cytoplasm Prostate stained with anti-PSMA Prostate tissue stained with Anti-PSA using DAB chromogen Ready-to-use (Manual): AM014-5ME Ready-to-use (Automated): i6000TM Xmatrx® AM014-10ME AX014-YCDE Concentrated: MU014-UCE Recommended Barrier Control: FB-014M RU O PSA is a glycoprotein with a molecular mass of 33-34 kD. Clone ErPr8 is directed against a 35 kD protein identical to PSA. PSA is biochemically and immunologically distinct from prostatic acid phosphatase. It is restricted to the cytoplasm of acinar and ductal epithelia of normal, benign or malignant prostate tissue. This antibody is useful for determining if an isolated metastasis is of prostatic origin. Since PSA is released by prostatic tumors, it is also a valuable serum marker of neoplasia. For research use only. Not for use in diagnostic procedures. pS2 Estrogen Inducible Protein P Clone: Membrane/Cytoplasm Pre-treatment: AR Citra Plus/EZ-AR 2 Manual/i6000: HK081-5KE Xmatrx: HX032-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN714-5M Recommended Positive Control: AN714-10M AY714-YCD NU714-UC FG-714N Recommended Barrier Control: FB-714N Prostate-specific membrane antigen (PSMA), also known as folate hydrolase 1 (FOLH1), is a type II transmembrane glycoprotein belonging to the M28 peptidase family. PSMA has two enzymatic activities, one as a prostate-specific integral membrane folate hydrolase and the other as a carboxypeptidase. An antibody to PSMA lables normal prostate epithelial cells and prostate tumor cells. Although the expression of PSMA in neovasculature of a variety of solid tumors has been reported, it is a useful marker for prostate tumors. In prostate cancer, overexpression of PSMA is correlated with high tumor grade, non-diploid tumors and advanced tumor state. It can be used as an effective predictor for tumor progression in prostate cancer. PS2.1 IgG1 Source: Mouse Immunogen: Synthetic peptide of 31 amino acid residues from the C-terminus of human pS2 protein Clone: RCC-26 Isotype: IgG1/K Source: Mouse pS2 protein Immunogen: Human RCC Localization: Cytoplasm Specificity: Renal Glycoprotein Pre-treatment: Protease/EZ-AR 1 Localization: Cytoplasm Pre-treatment: AR Citra Plus Manual/i6000: HK081-5KE Xmatrx: None Manual/i6000 EK002-5K Xmatrx: HX031-YCDE or EK002-5K Ready-to-use (Manual): AM190-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM190-10M AX190-YCD MU190-UC Recommended Positive Control: FG-190M Recommended Barrier Control: FB-190M This antibody specifically recognizes 6.5 kD human pS2 estrogenregulated protein (6.5 kD). pS2 is specifically expressed and secreted by ER-mucosa cells of the normal stomach (antrum and body) of both female and male individuals. Primary breast tumors have been shown to express pS2 in ER+primary breast tumors. This antibody shows a predominantly cytoplasmic localization of pS2 protein. 126 Localization: Isotype: Specificity: Breast carcinoma stained with Anti-pS2 estrogen inducible protein using Fast Red chromogen P ErPr-8 Clone: P - Tissue Type FFPE F - Tissue Type Frozen RCC (Renal Cell Carcinoma) P Renal Cell carcinoma stained with Anti-RCC using DAB chromogen Ready-to-use (Manual): AM543-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM543-10M AX543-YCD MU543-UC Recommended Positive Control: FG-543M Renal cell carcinoma, also known by a gurnistical tumor, is the most common form of kidney cancer arising from the renal tubule. RCC antibody recognizes a 200 kD glycoprotein localized in the brush border of the proximal renal tubule. It immunoreacts with approximately 90% of primary renal cell carcinomas and approximately 85% of metastatic renal cellcarcinomas. Other tumors that may react with this antibody are parathyroid adenoma, an occasional breast carcinoma. Nephroblastoma, oncocytoma, mesoblastic nephroma, transitional cell carcinoma, and angiomyolipoma are not labeled with this antibody www.biogenex.com Primary Antibodies S100-β S100 Protein P Melanoma stained with anti-S100 beta antibody using DAB EP32 Clone: 15E2E2 Isotype: IgG Isotype: IgG 2a Kappa Source: Rabbit Source: Mouse Immunogen: A synthetic peptide corresponding to residues on the C-terminus of human S100 Beta protein Immunogen: Purified bovine S-100 protein Specificity: S100 protein Localization: Cytoplasm & Nucleus Specificity: S100 Beta protein Pre-treatment: None Localization: Cytoplasm Pre-treatment: AR Citra/EZ-AR 2 Manual/i6000: HK087-5KE Xmatrx: HX032-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Melanoma stained with Anti-S100 using DAB chromogen AN713-5M Recommended Positive Control: AN713-10M AY713-YCD NU713-UC FG-713N Recommended Barrier Control: FB-713N S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100 Beta is abundant in glial cells of the central and peripheral nervous system, in melanocytes, chondrocytes, and adipocytes. It also labels Langerhans cells, histiocytes, epithelial, myoepithelial cells and integrating reticular cells of lymphoid tissue, and tumors originated from these cells. S100 Beta is a useful marker for diagnosis of melanoma, tumors of nerves system. S100 Protein P Melanoma stained with anti- S100 using AEC chromogen P Clone: Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® AM058-5M AM058-10M AX058-YCD Concentrated: Recommended Positive Control: MU058-UC FG-058M Recommended Barrier Control: FB-058M S100 protein is a low molecular weight soluble protein first isolated from the brain and initially believed to be exclusively a glial marker. Two subunits of S100 protein have been identified. The beta subunit is present in all S100 positive cells and tumors. In contrast, the alpha subunit is detectable only in neurons and lymph node macrophages. The presence of S100 protein is readily demonstrated in routinely processed malignant melanomas. S100 protein has also been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, in addition to glial cells. Neoplasms derived from these cells also express S100 protein to varying degrees. A large proportion of well-differentiated tumors of salivary gland, adipose, cartilaginous tissue, and Schwann cell-derived tumors express S100 protein. Clone: Polyclonal Source: Rabbit Immunogen: S-100 protein isolated polyclonal from bovine brain using affinity chromatography Clone: EP186 Isotype: IgG Source: Rabbit Specificity: S100 protein Immunogen: Localization: Cytoplasm & Nucleus Tissue Type: FFPE A synthetic peptide corresponding to residues of human S100P protein Pre-treatment: EZ-AR 1/EZ-AR 2 Specificity: S100P protein Manual/i6000: None Localization: Cytoplasm/Nucleus Xmatrx: HX031-YCDE/HX032-YCDE Pre-treatment: AR Citra Manual/i6000: HK087-5KE Xmatrx: None Ready-to-Use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Recommended Positive Control: S100P Lung stained with anti-S100p AR058-5R AR058-10R AW058-YCD PU058-UP FG-058P S100 protein is a low molecular weight soluble protein first isolated from the brain and initially believed to be exclusively a glial marker. Two subunits of S100 protein have been identified. and they are differently expressed by various cells. the beta subunit is present in all S100 positive cells and tumors. In contrast, the alpha subunit is detectable only in neurons and lymph node macrophages. The presence of S100 protein is readily demonstrated in routinely processed malignant melanomas. S100 protein also has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, in addition to glial cells. Neoplasms derived from these cells also express S100 protein to varying degrees. A large proportion of well-differentiated tumors of salivary gland, adipose, cartilaginous tissue, and Schwann cell derived tumors express S100 protein. internationalcs@biogenex.com P Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN712-5M Recommended Positive Control: AN712-10M AY712-YCD MU712-UC FG-712N Recommended Barrier Control: FB-712N S100P is a member of the S100 family of proteins. S100P is expressed in various normal tissues including placenta, bladder, spleen, gastric and intestinal mucosa. Overexpression of S100P has been detected in several cancers such as colon, prostate, pancreatic and lung carcinomas. It has been functionally implicated in carcinogenic processes. S100P is an early developmental marker of pancreatic carcinogenesis and can be used as a marker for pancreatic ductal adenocarcinoma. It may also serve as a predictor of distant metastasis and poor survival in non-small cell lung carcinomas. P - Tissue Type FFPE F - Tissue Type Frozen 127 Primary Antibodies Sarcomeric Actin Stat 5-Alpha (C-term) P Muscle tissue stained with AntiSarcomeric Actin using DAB as chromogen ZMSA-5 Clone: E289 Isotype: IgG Isotype: IgG Source: Mouse Source: Rabbit Immunogen: Mouse anti-sarcomeric actin antibody is purified from mouse ascites. Immunogen: A synthetic peptide corresponding to residues in C-terminus of human Stat-alpha Stat 5-Alpha (C-term) Specificity: Sarcomeric Actin Localization: Cytoplasm Specificity: Pre-treatment: EZ-ARTM 1 Localization: Cytoplasm & Nucleus Manual/i6000: HK521-XAK Pre-treatment: EZ-AR™ 1/EZ-AR 2 Xmatrx: HX031-YCDE Manual/i6000: HK521-XAK Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): AM511-5M Ready-to-use (Automated): i6000TM Xmatrx® AM511-10M AX511-YCD Concentrated: Recommended Positive Control: MU511-UC FG-511M Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as an antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin. This antibody is specific for alpha isoform of skeletal and cardiac muscle actin. This antibody shows wide cross reactivity to other tissues from human, sheep, rabbit, guinea pig, rat, frog and snake. However, it does not react with smooth muscle tissue. Secretin P Stomach tissue stained with Anti-Secretin stained with AEC chromogen Breast carcinoma stained with AntiStat 5-alpha using DAB chromogen Ready-to-use (Manual): AN480-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN480-10M AX480-YCD NU480-UC Recommended Positive Control: FG-480N Stat 5 is part of a 7 protein family known as signal transducer and activator of transcription (STAT) which contributes to signal transduction by cytokine, hormone and growth factor. Signal transducer and activator of transcription 5 (Stat 5) functions in signal translocated to the nucleus in response to phosphorylation. Stat 5-alpha tyrosine phosphorylation is activated predominantly by IL-2 but also by IL-3, IL-5, IL-7, IL-9, IL-15, G-CSF and GM-CSF. Tyrosine phosphorylation is required for maximal transcriptional activity. NCoA-1/SRC-1 acts as coactivator for both the alpha and beta isoforms of Stat 5. Clone: Polyclonal Source: Rabbit Immunogen: Synthetic porcine secretin coupled to keyhole limpet hemocyanin with carbodiimide; conjugate emulsified in Freund’s complete adjuvant prior to injection Clone: Polyclonal Source: Rabbit Immunogen: Synthetic Substance P bound to keyhole limpet hemocyanin (KLH) Specificity: Substance P Specificity: Secretin Localization: Cytoplasm Localization: Cytoplasm Pre-treatment: EZ-AR 1/EZ-AR 2 Pre-treatment: EZ-AR 1 Xmatrx: HX031-YCDE/HX032-YCDE Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Recommended Positive Control: AR067-5R AW067-YCD FG-067P This hormone, a polypeptide of 27 amino acids, which acts to stimulate pancreatic bicarbonate, is localized primarily in the gastrointestinal tract. It is released from secretin cells (S-cells) which have been localized within the antropyloric, duodenal, jejunal and ileal mucosa of human tissue. Hypersecretinemia has been observed in duodenal ulcers, Zollinger-Ellison syndrome, and chronic renal failure. This antibody stains Secretin in cellular elements in the epithelium of the gastrointestinal tract. 128 P Clone: P - Tissue Type FFPE F - Tissue Type Frozen Substance P P Brain tissue stained with AntiSubstance P using AEC chromogen Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Concentrated: AR069-5R AW069-YCD PU069-UP Recommended Positive Control: FG-069P Substance P is one of several neuroendocrine polypeptides localized in both the nervous system and gastrointestinal tract. Substance P is grouped into a family with bombesin and neurotensin because all three peptides are located in both brain and gut and terminate with a common dipeptide sequence (-Leu-Met-NH 2 ) at the amino terminal end. Substance P is found in most mid-gut and about half of foregut and hind-gut intestinal carcinoids. This antibody cross-reacts with other species including chicken and opossum. This antibody stains Substance P in nerve fibers. www.biogenex.com Primary Antibodies Survivin Tau P Bladder carcinoma stained with anti-Survivin using DAB chromogen P Clone: EP2880Y Clone: Tau-2 Isotype: IgG Isotype: IgG1 Source: Rabbit Source: Mouse Immunogen: A synthetic peptide corresponding to residues in the N-terminus of Human Survivin Immunogen: Purified bovine Microtubule Associated Protein Tau (MAPT) Specificity: Tau protein Specificity: Survivin Localization: Cytoplasm Localization: Nucleus/Cytoplasm Pre-treatment: AR Citra Plus/EZ-AR 1 Pre-treatment: EZ-AR™1/EZ-AR 2 Manual/i6000: HK081-5KE Manual/i6000: HK521-XAK Xmatrx: HX031-YCDE Xmatrx: HX032-YCDE Ready-to-use (Manual): Cerebellum stained with Anti-Tau using DAB chromogen AN503-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AN503-10M AX503-YCD NU503-UC Recommended Positive Control: FG-503N Survivin is a unique member of the inhibitor of apoptosis (IAP) protein family that interferes with post-mitochondrial events including activation of caspases. Survivin regulates cell cycle and is expressed in most tumors, but it is barely detectable in the terminally differentiated normal cells and tissues. The differential expression of Survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Synaptophysin P Cerebellum stained with Anti-Synaptophysin using AEC chromogen Clone: Snp88 Isotype: IgG3 Kappa Source: Mouse Immunogen: Recombinant human synaptophysin Specificity: Ready-to-use (Manual): AM412-5M Ready-to-use (Automated): i6000TM Xmatrx® AM412-10M AX412-YCD Concentrated: MU412-UC Recommended Positive Control: FG-412M Recommended Barrier Control: FB-412M Tau’s major role is to regulate neuronal microtubule assembly and stability thus playing a major role in movement disorders. Neurofibrillary tangles (NFTs), one of the histopathological signs of Alzheimer’s Disease, contain a lot of incorporated Tau protein. Anti-Tau antibody shows strong positive staining in NFT areas and may also stain pick bodies in Pick’s Disease. In normal tissue, the antibody may stain neurons and axons in the brain and spinal cord. This antibody stains the cytoplasm of neurons and its connected axon. Tau P Clone: Tau-5 Isotype: IgG1 Synaptophysin protein Source: Mouse Localization: Cytoplasm Immunogen: Human Tau Pre-treatment: AR Citra Plus/EZ-AR 1 Specificity: Tau Manual/i6000: HK081-5KE Localization: Cytoplasm Xmatrx: HX031-YCDE Pre-treatment: EZ-ARTM 1 Manual/i6000: HK521-XAK Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Tau expression in normal brain tissue stained using DAB chromogen AM363-5M Concentrated: AM363-10M AX363-YCD MU363-UC Recommended Barrier Control: FB-363M Recommended Positive Control: FG-363M Synaptophysin, a 38 kD glycoprotein, is the major integral membrane protein of synaptic vesicles. It is a sensitive quantitative molecular marker of synaptic density and also a useful marker in the identification and characterization of neuronal and neuroendocrine neoplasms of the adrenal medullary, pituitary, thyroid and islet cell tumors, gastrointestinal, bronchial, thymic and pancreatic carcinoid tumors. Immunohistochemistry of synaptophysin has been used in the evaluation of functional bowel disorders, cortical epileptogenesis, schizophrenia and amyotropic lateral sclerosis. internationalcs@biogenex.com Ready-to-use (Manual): AM459-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM459-10M AX459-YCD Recommended Positive Control: Recommended Barrier Control: MU459-UC FG-459M FB-459M This antibody recognizes proteins of 45-68 kD, identified as tau proteins. The Tau monoclonal antibody reacts with the non-phosphorylated as well as the phosphorylated forms of tau. Tau proteins are members of the microtubule associated proteins (MAPs) that stabilize neuronal microtubules in cell processes, establishment of cell polarity and intracellular transport. Six isoforms, ranging from 352 to 441 amino acids, are generated from a single Tau gene by alternative splicing in the human central nervous system. In Alzheimer’s disease, abnormally phosphorylated, tau proteins aggregate into paired helical filaments and loose their ability to maintain the microtubule tracks. Missense Tau mutations in individuals with a type of frontotemporal dementia, FTDP 17, have been discovered. P - Tissue Type FFPE F - Tissue Type Frozen 129 Primary Antibodies Terminal Deoxynucleotidyl Transferase (TdT) TFPU.1 P Thymoma stained with Anti-Terminal deoxynucleotidyl Transferase (TdT) using AEC chromogen P Clone: EPR3159Y Clone: TdT88 Isotype: IgG Isotype: IgG Source: Rabbit Source: Mouse Immunogen: Immunogen: Peptide containing specific sequence for N-terminal of human TdT protein A synthetic peptide corresponding to residues on the Cterminus of transcription factor PU.1 Specificity: TFPU Specificity: TdT Localization: Nucleus Pre-treatment: AR Citra/EZ-AR 1 Manual/i6000: Xmatrx: Ready-to-use (Manual): Localization: Nuclear Pre-treatment: EZ-AR™ 1/EZ-AR 2 HK087-5KE Manual/i6000: HK521-XAK HX031-YCDE Xmatrx: HX032-YCDE AM373-5M Ready-to-use (Automated): i6000TM Xmatrx® AM373-10M AX373-YCD Concentrated: MU373-UC This antibody identifies a 58 kD peptide normally found in cortical thymocytes and immature bone marrow lymphocytes. TdT expression has been reported to occur in a majority of cases of acute lymphocytic leukemia (ALL) cases. TdT staining is found in all subtypes of ALL with the exception of pre-B-cell ALL. TdT positivity has also been observed in approximately one third of all cases of chronic myeloid leukemia. TdT positive staining is found in ALL, acute myeloid leukemia and chronic myeloid leukemia. This antibody stains predominantly nuclear TdT in normal and neoplastic cells. Terminal Deoxynucleotidyl Transferase (TdT) P Clone: Polyclonal Source: Mouse Immunogen: Synthetic peptide Specificity: TdT antigen Localization: Nucleus Pre-treatment: AR Citra/EZ-AR 2 Manual/i6000: HK087-5KE Xmatrx: Lymphoma stained with Anti-TFPU using DAB chromogen Ready-to-use (Manual): AN520-5M Ready-to-use (Automated): i6000TM Xmatrx® AN520-10M AX520-YCD Concentrated: NU520-UC Recommended Positive Control: FG-520N Transcription factor PU.1 is a member of the Ets family of transcription factors and is required for the development of multiple hematopoietic lineages. It plays a pivotal role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development. The function of PU.1 is downregulated by AML1-ETO in myeloid leukemia, whereas overexpression of PU.1 restores normal differentiation. The lack of transcription factor PU.1 protein expression in classical Hodgkin’s disease (cHD), a lymphoproliferative disease of predominantly B-cell origin, likely contributes to the lack of immunoglobulin expression and incomplete B-cell phenotype characteristic of the Reed-Sternberg cells in cHD. Thyroglobulin P HX032-YCDE Thymoma stained with Anti-Terminal deoxynucleotidyl Transferase (TdT) using AEC chromogen Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Recommended Positive Control: AR373-5R AR373-10R AW373-YCD FG-373P Terminal deoxynucleotidyl transferase (TdT) is a nuclear protein widely used as a marker for evaluation and classification of acute leukemia. In the study of small round cell tumors, TdT is often used as a marker for lymphoblastic lymphomas and leukemias. TdT is widely used to stain apoptotic cells. Clone: 2H11 Isotype: IgG1 Source: Mouse Immunogen: Purified human thyroglobulin Specificity: Thyroglobulin Localization: Cytoplasm Pre-treatment: None Follicular adenoma stained with anti-Thyroglobulin using AEC chromogen Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® AM032-10M AX032-YCD Concentrated: MU032-UC Recommended Positive Control: FG-032M AM032-5M Thyroglobulin is a 19S glycoprotein with a molecular mass of approximately 650 kD. It constitutes 85-100% of the total of all thyroid iodoproteins. Immunohistochemical studies of thyroid carcinomas have revealed that a high portion of differentiated thyroid carcinomas synthesize thyroglobulin. Positive thyroglobulin staining indicates thyroidal origin of the tumor. Immunohistochemical and electron microscopic findings have disclosed a wide range of cellular differentiation in thyroid adenomas. 130 P - Tissue Type FFPE F - Tissue Type Frozen www.biogenex.com Primary Antibodies Thyroid Stimulating Hormone (TSH) Thyroid Transcription Factor-1 (TTF-1) P P Clone: 5404 Clone: 8G7G3/1 Isotype: IgG1 Kappa Isotype: IgG1 Kappa Source: Mouse Source: Mouse Immunogen: TSH Immunogen: Specificity: Thyroid Stimulating Hormone (TSH) Recombinant TTF-1 protein Specificity: TTF-1 antigen Localization: Cytoplasm Localization: Nucleus Pre-treatment: None Pre-treatment: AR Citra Plus/EZ-AR 1 Pituitary cell showing cytoplasmic positivity for TSH stained using DAB chromogen Thyroid carcinoma cells expressing nuclear positivity for TTF1 stained using DAB chromogen Manual/i6000: HK081-5KE Xmatrx: HX031-YCDE Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® AM033-5M Ready-to-use (Manual): AM397-5M AM033-10M AX033-YCD Ready-to-use (Automated): i6000TM Xmatrx® AM397-10M AX397-YCD Concentrated: MU033-UC Concentrated: MU397-UC Recommended Positive Control: FG-397M Thyrotrophs produce Thyroid Stimulating Hormone (TSH). TSH is a 28 kD glycoprotein that contains 201 amino acid residues and is composed of alpha and beta subunits. The alpha subunit (MW 13kD) is immunologically similar to the alpha subunit of the other anterior pituitary hormones. The beta subunit is unique to TSH and is responsible for the specific biological activity of TSH. To identify thyrotrophs without cross-reactivity with gonadotrophs, antibodies directed to the TSH beta subunit must be used. This antibody stains TSH and b-TSH in cytoplasm of postive cells. Thyroid Stimulating Hormone (TSH) Thyroid Transcription Factor-1 (TTF-1) is a 40 kD protein that is a member of NKx2 family of homeodomain transcription factors that regulates the expression of thyroid- and lung-specific genes. It is a very selective marker for adeno carcinomas of lung and thyroid origin. Nuclear localization of this protein is seen in the epithelial cells of thyroid gland and lung. The anti-TTF-1 antibody is a useful tool for differentiating pulmonary adeno carcinoma from metastatic breast carcinoma and mesothelioma. This antibody stains TTF-1 antigen located in the nucleus of thyroid and lung normal and carcinoma cells and other positive cells. P Clone: Polyclonal Source: Rabbit Immunogen: Purified TSH from human pituitary gland Specificity: Thyroid Stimulating Hormone (TSH) Localization: Cytoplasm Pre-treatment: EZ-AR 1 Xmatrx: HX031-YCDE Thyroxine P Pituitary cell showing cytoplasmic positivity for TSH stained using AEC chromogen Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® AR033-5R Thryroid tissue stained with AntiThyroxine using AEC chromogen AW033-YCD Thyrotrophs produce Thyroid Stimulating Hormone (TSH). TSH is a 28 kD glycoprotein that contains 201 amino acid residues and is composed of alpha and beta subunits. The alpha subunit (MW 13kD) is immunologically similar to the alpha subunit of the other anterior pituitary hormones. The beta subunit is unique to TSH and is responsible for the specific biological activity of TSH. To identify thyrotrophs without cross-reactivity with gonadotrophs, antibodies directed to the TSH beta subunit must be used. This antibody stains positive for TSH in cytoplasm of thyrotrophs. internationalcs@biogenex.com Clone: D5 Isotype: IgG1 Source: Mouse Immunogen: Me-Thyroxine conjugated to bovine serum albumin Specificity: Thyroxine (T4) Localization: Cytoplasm Pre-treatment: EZ-AR 1/EZ-AR 2 Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Concentrated: AM034A-5M AX034-YCD MU034A-UC Recommended Positive Control: FG-034M The main hormones produced by the thyroid are Thyroxine (T4 or tetraiodothyronine) and, on a much smaller scale, triiodothyronine (T3). T4 and T3 have been demonstrated in normal and neoplastic thyroid follicular cells. In thyroid cancer, however, the iodine content may be 1/100 that of normal thyroid tissue, whereas thyroglobulin is much more abundant, occurring at 1/2 to 1/3 that of a normal thyroid. This antibody stains colloid in thyroid follicle and cytoplasm of thyroid follicular cells. P - Tissue Type FFPE F - Tissue Type Frozen 131 Primary Antibodies TIA-1 Toxoplasma gondii P Anaplastic large T Cell Lymphoma stained with Anti-TIA using DAB chromogen Clone: 2G9A10F5 Isotype: IgG Source: Mouse Immunogen: Human bone marrow malignant cells from a non-B, non-T acute leukemia Polyclonal Source: Rabbit Immunogen: This antibody was produced by immunization of rabbits with live organisms of Toxoplasma gondii strain C56. TIA-1 Localization: Cytoplasm Specificity: Toxoplasma gondii Pre-treatment: EZ-AR™ 1 Localization: T. gondii Manual/i6000: HK521-XAK Xmatrx: HX031-YCDE AM529-5M Ready-to-use (Automated): i6000TM Xmatrx® AM529-10M AX529-YCD Concentrated: MU529-UC The T cell intracellular antigen 1 (TIA-1) is a 17-kD cytoplasmic granule associated protein also designated as GMP-17, for granule membrane protein of 17 kD. The GMP-17/TIA-1 molecule is expressed in cells possessing cytolytic potential and could be involved in the signaling cascade of Fas (CD95)-mediated apoptosis. Within hematopoietic cell lines, the 2G9 monoclonal antibody (mAb) reacts with about 90% of CD16+, 50 – 60% of CD8+, and less than 10% of CD4+ normal peripheral blood lymphocytes. It reacts with almost all monocytes and granulocytes. This antibody also reacts with CD4+ activated T-cell clones, activated NK cell clones, and Con A activated thymocytes, but not with B lymphocytes or B-cell lines. Infected cells stained with AntiToxoplasma using DAB chromogen Ready-to-use (Manual): AR125-5RE Concentrated: PU125-UPE Recommended Positive Control: FG-125P RU O Toxoplasma gondii is a common protozoan parasite of humans and vertebrate animals. In human hosts with a competent immune system, T. gondii infection generally develops into an asymptomatic chronic infection, with the organism sequestered in dormant “tissue cysts” often for the lifetime of the host. However, in immunocompromised hosts, such as AIDS patients or organ transplant recipients receiving immunosuppressive therapy, infection can lead to toxoplasmosis. The disease is characterized by the proliferation of T. gondii tachyzoites, which can damage the central nervous system, and can be fatal if untreated. During acute infection, T. gondii tachyzoites may be found in the bloodstream (but not within RBCs), in tissue, or within various cells, especially reticuloendothelial cells, leukocytes, and epithelial cells. This antibody stains Toxoplasma gondii in the cytoplasm of infected cells or tissues stained by immunohistochemical techniques. For research use only. Not for use in diagnostic procedures. P Breast tissue stained with Anti-Top 2 Alpha using DAB as chromogen Clone: EP1102Y Isotype: IgG Source: Rabbit Immunogen: A synthetic peptide corresponding to C-terminal residues of human Topo 2 Alpha Specificity: Topo 2 Alpha Localization: Nucleus/Cytoplasm Pre-treatment: AR Citra Plus/EZ-AR 2 Manual/i6000: HK081-5KE Xmatrx: HX032-YCDE Ready-to-use (Manual): AN576-5M Ready-to-use (Automated): i6000TM Xmatrx® AN576-10M AX576-YCD Concentrated: NU576-UC Recommended Positive Control: FG-576N Topoisomerase IIa (Topo 2α) is a ubiquitously expressed enzyme that regulates DNA topology in a cell. Because of its ability to unwind the DNA helicase, Topo 2α is an enzyme essential for DNA replication and protein synthesis. It has been linked to cell proliferation and mitotic processes. Topo 2α overexpression has been linked to a number of human malignancies. Topo 2α has been further identified as a useful marker in evaluating breast and thyroid malignant tumors. 132 Clone: Specificity: Ready-to-use (Manual): Topo 2 Alpha P P - Tissue Type FFPE F - Tissue Type Frozen Transferrin P Clone: HT1/13.6.3 Isotype: IgG1 Source: Mouse Immunogen: Transferrin Specificity: Transferrin Localization: Cytoplasm Pre-treatment: EZ-AR 1 Xmatrx: HX031-YCDE Liver tissue stained with AntiTransferrin using AEC chromogen Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Recommended Positive Control: AM025-5M AX025-YCD FG-025M Human transferrin, an iron-binding protein, is produced mainly in the liver, and can be demonstrated within hepatocytes. Transferrin has also been demonstrated by immunohistology in a wide variety of other tissues including stomach, duodenum, gall bladder, thyroid, kidney, male and female reproductive tracts, skin, and in histiocytes. Such widespread occurrence of transferrin suggests evidence for the diverse roles that it may play such as iron transport across intestinal mucosa, intracellular iron transport, and providing non-specific immunity against micro-organisms by chelating free iron. www.biogenex.com Primary Antibodies Transforming Growth Factor (TGF), Alpha Tumor-Associated Glycoprotein (TAG-90, BCA) P Breast carcinoma showing TGF positivity stained using AEC chromogen P Clone: TGF88 Clone: B6.2 Isotype: IgG1 Isotype: IgG1 Source: Mouse Source: Mouse Immunogen: Synthetic peptide representing a unique epitope to pro-TGF-α covalently bound to keyhole limpet hemocyanin Immunogen: Membrane-enriched fraction of breast tumor metastatic to the liver Specificity: 90 kD tumor-associated glycoprotein Specificity: Transforming growth factor, alpha (TGF-α ) Localization: Cytoplasm Pre-treatment: EZ-AR 1 Xmatrx: HX031-YCDE Ready-to-use (Manual): AM377-5M Ready-to-use (Automated): Xmatrx® AX377-YCD Concentrated: MU377-UC Breast carcinoma stained with Anti-TAG-90 (BCA) using DAB chromogen Transforming Growth Factor, alpha (TGF-α) is a 50 amino acid peptide that is involved in the regulation of normal and malignant cell growth. The mature peptide is released following proteolytic cleavage from a 160 amino acid transmembrane precursor molecule. It is one of the various ligands for EGFR and seem to be involved in the growth regulation of intestinal mucosa and might be related to the development and progression of gastrointestinal tumors. Macrophages secrete TGF-α to trigger proliferation of cancer cells. TGF-α is synthesized by several cells, like epidermal keratinocytes, fibroblasts, and cells of hematopoetic origin like eosinophils and simulated macrophages. Manual: EK000-5K Xmatrx: HX031-YCDE or EK000-5K Concentrated: MU005-UC Recommended Positive Control: FG-005M AX005-YCD Clone B6.2 recognizes a 90 kD glycoprotein in mammary carcinomas, metastatic lymph nodes, lung carcinomas, and adenocarcinomas. This antibody reacts intensely with tumor cells, yet is unreactive with cells in normal tissue. This antibody reacts equally with breast cancer, breast fibroadenoma, lobular carcinoma of the breast, duct carcinoma of the breast, and lung carcinoma. It also reacts with gastric and papillary adenocarcinomas, and adenocarcinoma of the colon, ovary, pancreas, lung and prostate. This antibody stains positive in the cytoplasm of tumor cells. Tyrosinase P Clone: B72.3 Isotype: IgG1 Source: Mouse Immunogen: Membrane-enriched fraction of a breast carcinomaderived from a liver metastasis Specificity: Tumor-Associated Glycoprotein (TAG-72) Localization: Cytoplasm Pre-treatment: None AM054-5M AM054-10M AX054-YCD MU054-UC FG-054M FB-054M Tumor-Associated Glycoprotein 72 (TAG-72) is an oncofetal mucin antigen expressed by normal secretory endometrium and most human adenocarcinomas, including colorectal, gastric, pancreatic, mammary, and ovarian. This antigen is expressed by invasive ductal breast carcinomas, colon, pancreatic, gastric, esophageal, lung, ovarian and endometrial adenocarcinomas. It is not expressed by leukemias, lymphomas, sarcomas, mesotheliomas, melanomas, or benign tumors. This antigen is also expressed on normal secretory endometrium, but not on other normal tissues. This antibody stains positive in the cytoplasm of specific carcinoma cells. internationalcs@biogenex.com Pepsin/EZ-AR 1 AM005-5M P Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Recommended Positive Control: Recommended Barrier Control: Cytoplasm Pre-treatment: Ready-to-use (Manual): Ready-to-use (Automated): Xmatrx® Tumor-Associated Glycoprotein (TAG-72) Breast carcinoma stained with Anti-TAG-72 (BCA) using DAB chromogen Localization: Melanoma stained with AntiTyrosinase using DAB chromogen Clone: Ty/G5 Isotype: IgG2a Source: Mouse Immunogen: Human Tyrosinase Specificity: Tyrosinase Localization: Cytoplasm Pre-treatment: EZ-AR™ 1 Manual/i6000: HK521-XAK Xmatrx: HX031-YCDE Ready-to-use (Manual): AM535-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM535-10M AX535-YCD MU535-UC Recommended Positive Control: FG-535M Tyrosinase is a copper-containing enzyme present in plant and animal tissues that catalyzes the production of melanin and other pigments from tyrosine by oxidation. The gene for tyrosinase is regulated by the microphthalmia-associated transcription factor. A mutation in the tyrosinase gene resulting in impaired tyrosinase production results in type I oculocutaneous albinism, a hereditary disease that one in every 17,000 person has in the US. Anti-tyrosinase has been found to be quite specific for melanotic lesions such as malignant melanoma, and melanotic neurofibroma. Essentially no carcinomas express this marker. P - Tissue Type FFPE F - Tissue Type Frozen 133 Primary Antibodies VEGF Vimentin P Angiosarcoma stained with AntiVEGF using DAB chromogen P Clone: Polyclonal Clone: V9 Source: Rabbit Isotype: IgG1 Immunogen: Human recombinant VEGF165 Source: Mouse Immunogen: Vimentin purified from porcine eye lens Vimentin Specificity: VEGF Localization: Cytoplasm Specificity: Pre-treatment: EZ-AR™ 1 Localization: Cytoplasm Manual/i6000: HK521-XAK Pre-treatment: None Xmatrx: HX031-YCDE Ready-to-use (Manual): AR483-5R Skin stained with Anti-Vimentin Antibody using DAB chromogen Ready-to-use (Manual): AM074-5M AR483-10R AW483-YCD Ready-to-use (Automated): i6000TM Xmatrx® AM074-10M AX074-YCD Concentrated: PU483-UP Concentrated: MU074-UC Recommended Positive Control: FG-483P Recommended Positive Control: FG-074M Recommended Barrier Control: FB-074M Ready-to-use (Automated): i6000TM Xmatrx® Vascular endothelial factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGF receptors stimulate the proliferation of endothelial cells, induce angiogenesis, and increase vascular permeability in both large and small vessels. The mitogenic activity of VEGFs appears to be mediated by specific VEGF receptors. Villin P Intestine stained with Anti-Villin using DAB as chromogen Clone: ID2C3 Isotype: IgG Source: Mouse Immunogen: Recognizes porcine, chicken and human villin from cellular extract of the intestinal Cell line HT29 and from human renal epithelial cells Vimentin, Non-Hematopoietic P Clone: LN6 Isotype: IgM Source: Mouse Specificity: Villin Localization: Cytoplasm/Brush border Pre-treatment: EZ-ARTM 2/EZ-AR 1 Manual/i6000: HK522-XAK Localization: Cytoplasm Xmatrx: HX031-YCDE Pre-treatment: None Ready-to-use (Manual): AM525-5M Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AM525-10M AX525-YCD MU525-UC Recommended Positive Control: FG-525M Villin is a cytoskeletal protein of microvilli of epithelial cell brush borders found in absorptive cells of the intestine and proximal renal tubes. Villin is a very specific marker for gastrointestinal tumors and adenocarcinomas of the pancreas. Other subsets of tumors stained with Villin are Merkel cell, lung (with rootlets), ovarian and kidney. It does not stain breast cancer. Used in a panel with CK7, CK20/CDX2 and TTF-1, Villin can be very useful in differentiating colon adenocarcinoma from breast carcinoma and from lung adenocarcinoma. 134 Vimentin is the major intermediate filament in a variety of mesenchymal or mesenchymally derived non-muscle cell types. Vimentin is found in all types of sarcomas and lymphomas. Positive staining for vimentin is seen in most cells of fibrosarcomas, liposarcomas, malignant fibrous histocytomas, angiosarcomas, chondrosarcomas and lymphomas. When the vimentin antibody is used in combination with other antibodies as a panel, it can aid in the histological classification of normal and malignant tissues. This antibody immunohistochemically labels a variety of mesenchymal cells. P - Tissue Type FFPE F - Tissue Type Frozen Immunogen: Human Thymic Nuclei Specificity: Non-hematopoietic vimentin Leiomyoma stained with antiVimentin using DAB chromogen Ready-to-use (Manual): AM163-5M Ready-to-use (Automated): Xmatrx® AX163-YCD Concentrated: MU163-UC Recommended Positive Control: FG-163M LN6 recognizes a unique epitope of vimentin, a 60 kD protein, not expressed in cells of hematopoietic derivation. LN6 can be useful in the immunohistological study of soft tissue disorders. It reacts strongly with sarcomas, melanomas and meningomas. LN6 does not, however, stain leukocyte common antigen-positive tissues such as lymphomas and leukemias. In normal tissue, LN6 stains endothelium, muscle, fibroblasts, melanocytes, peripheral nerve, Sertoli cells, kidney mesangial cells and tubules, osteoblasts and periosteum. This antibody stains non-hematopoietic form of Vimentin in human sarcomas and normal cells of mesenchymal derivation but is nonreactive with cells of hematopoietic derivation. www.biogenex.com Primary Antibodies VIP ZAP-70 P Intestine tissue stained with Anti-VIP using DAB as chromogen Clone: Polyclonal Isotype: IgG Source: Rabbit Immunogen: Synthetic peptide corresponding to full length mature vasoactive intestinal peptide conjugated to Keyhole Limpet Haemocyanin Specificity: VIP Localization: Cytoplasm Pre-treatment: EZ-ARTM 2/EZ-AR 1 Manual/i6000: HK522-XAK Xmatrx: HX031-YCDE Ready-to-use (Manual): AR530-5R Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: AR530-10R AW530-YCD PU530-UP Recommended Positive Control: FG-530P Tonsil tissue stained with AntiZAP-70 using DAB chromogen Wilm’s tumor stained with AntiWilm’s tumor using DAB chromogen CAN-R9(IHC)-56-2 Isotype: IgG Source: Rabbit Immunogen: A recombinant protein corresponding to N-terminus of human WT Localization: Nucleus Pre-treatment: AR Citra Plus/EZ-AR 2 Manual/i6000: HK081-5KE Xmatrx: HX032-YCDE Ready-to-use (Manual): AN584-5M Ready-to-use (Automated): i6000TM Xmatrx® AN584-10M AX584-YCD Concentrated: NU584-UC Recommended Positive Control: FG-584N ZAP-70 Source: Mouse Immunogen: Human ZAP-70 Specificity: ZAP-70 Localization: Cytoplasm/Membrane Pre-treatment: EZ-AR™ 1 Manual/i6000: HK521-XAK Xmatrx: HX031-YCDE AM544-5M AM544-10M AX544-YCD MU544-UC FG-544M P T Cells showing ZAP-70 expression in tonsillar tissue using AEC as chromogen Wilm’s tumor protein is a transcription factor that contains four zinc-finger motifs, it is essential for correct mammalian urogenital development. The WT1 gene, located on chromosome 11p13, is inactivated in 5 to 10% of sporadic Wilms’ tumors and nearly 100% of Denys-Drash patients. The protein encoded by WT1 regulates transcription of other genes and can function both as a transcriptional activator and repressor. The WT1 gene is normally expressed in fetal kidney and mesothelioma, and its expression has been suggested as a marker for Wilm’s tumor and mesothelioma. internationalcs@biogenex.com IgG2a Recommended Positive Control: Clone: Wilm’s tumor protein ZAP70-C3 Isotype: ZAP-70 is an abbrevation for Zeta-chain-associated protein kinase 70 (70 is the molecular weight in kD). The protein is a member in the proteintyrosine kinase family. ZAP-70 protein is expressed in leukemic cells of approximately 25% of Chronic Lymphocytic Leukemia (CLL) cases. ZAP-70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (ZAP-70 negative) and Ig-unmutated (ZAP-70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The ZAP-70 positive Ig-unmutated CLL cases have a poorer prognosis. P Specificity: Clone: Ready-to-use (Manual): Ready-to-use (Automated): i6000TM Xmatrx® Concentrated: Vasoactive intestinal peptide (VIP) is a 28 amino acid neuropeptide that has been isolated from various organs like intestine, the brain, upper respiratory and nasal mucosa, salivary glands, and the male and female genital tracts. It is also identifiable in human eosinophils, polymorphonuclear and mononuclear leucocytes. VIP is also known as a potent stimulant of mucous secretion, vasodilatation, and smooth muscle relaxation in bronchus and many other organs. According to various studies, VIP also has effects on the immune regulation. VIP is known to have inhibited the proliferative response of T lymphocytes to mercuric chloride, and inhibited natural killer (NK) cell function. Wilm’s Tumor P Clone: YE291 Isotype: IgG Source: Rabbit Immunogen: ZAP-70 antigen Specificity: ZAP-70 Localization: Cytoplasm/Membrane Pre-treatment: EZ-ARTM 1/EZ-AR 2 Manual/i6000: HK521-XAK Xmatrx: HX031-YCDE/HX032-YCDE Ready-to-use (Manual): AN466-5M Ready-to-use (Automated): i6000TM Xmatrx® AN466-10M AX466-YCD Concentrated: NU466-UC Recommended Positive Control: FG-466N ZAP-70 (70-kDa zeta-associated protein) is an intracellular tyrosine kinase discovered initially because of its role in T-cell signaling. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined. P - Tissue Type FFPE F - Tissue Type Frozen 135 Primary Antibodies 136 P - Tissue Type FFPE F - Tissue Type Frozen www.biogenex.com IHC Detection Systems IHC Detection Systems Super Sensitive™ IHC Detection Systems Immunohistochemistry is a highly sensitive method that allows the localization of antigen within a cell or a tissue with high resolution. The method is based on the use of a primary antibody that specifically binds to its complementary antigen. The bound antibody may then be visualized by a variety of methods such as colorimetric end points. BioGenex offers three basic types of IHC Detection Systems: I. Super SensitiveTM Polymer-HRP IHC Detection System II. Super SensitiveTM One-step Polymer-HRP Detection system III.Super SensitiveTM Link-Label IHC Detection System I. Super SensitiveTM Polymer-HRP IHC Detection System This is a novel detection system using a non-biotin polymeric technology that makes use of two major components: Super Enhancer and a Poly-HRP reagent. As the system is not based on the biotin-avidin system, problems associated with endogenous biotin are completely eliminated. The technology allows excellent cell penetration ability for intense nuclear, cytoplasmic and membrane antigen staining, compared with other polymer-HRPs. Benefits • Clean stain without endogenous biotin background • High signal to noise ratio for intense stain • Excellent sensitivity for weakly expressed antigens • Universal system for rabbit and mouse antibodies • Higher dilution of antibodies 138 www.biogenex.com IHC Detection Systems IA. Manual Use Product Name Pack size Contents Cat. No. 60 Slides Super Enhancer Reagent (6.0 ml), Polymer-HRP Reagent (6.0 ml), QD400-60KE Ready-to-use Detection System Super Sensitive™ Polymer-HRP IHC Detection System/DAB Stable DAB Substrate Buffer (10 ml), Liquid DAB chromogen (2.0ml), Negative Control for Mouse (3.0 ml), Negative Control Rabbit (3.0ml), Peroxide Block (6.0 ml), Power Block™ (6.0 ml) Ready-to-use Detection System (Large Volume) Super Sensitive™ Polymer-HRP IHC Detection System/DAB Large Volume 500 Slides Super Enhancer Reagent (50 ml), QD420-YIKE Polymer-HRP Reagent (50 ml), Stable DAB Substrate Buffer (50 ml), Liquid DAB Chromogen (5.0ml) Ready-to-use Detection System (Mega Volume) Super Sensitive™ Polymer-HRP IHC Detection System/DAB Mega Volume 1000 Slides Super Sensitive™ Polymer-HRP IHC Detection System Mega Volume 1000 Slides Super Enhancer Reagent (100 ml), Polymer-HRP Reagent (100 ml), QD430-XAKE Stable DAB Substrate Buffer (100 ml), Liquid DAB chromogen (10ml) Super Enhancer Reagent (100 ml), Polymer-HRP Reagent (100 ml) QD440-XAKE II. Super Sensitive™ One-step Polymer-HRP Detection Systems † The detection system is designed with proprietary technology, provides superior sensitivity, specificity and very short protocol. The innovative secondary antibody-polymer conjugate consisting of multiple small HRP active-site, enabling clean and intense, nuclear, cytoplasmic and membrane stain. This technology completely eliminates background staining caused by endogenous biotin in tissues especially kidney, liver, spleen, colon, stomach and brain. Benefits • One system does it all - mouse and rabbit • Easy and fast staining in 15 minutes • Clean and intense stain without endogenous biotin background • Higher dilution of antibodies IIA. Manual Use Product Name Pack size Contents Cat. No. Polymer-HRP Reagent (50 ml), QD620-YIKE Ready-to-use Detection System (Large Volume) Super SensitiveTM One-step Polymer-HRP Detection Kit/DAB, Large Volume 500 Slides Stable DAB Substrate Buffer (50 ml), Liquid DAB chromogen (5.0 ml) Ready-to-use Detection System (Mega Volume) Super SensitiveTM One-step Polymer-HRP Detection Kit/DAB, Mega Volume internationalcs@biogenex.com 1000 Slides Polymer-HRP Reagent (100 ml), Stable DAB Substrate Buffer (100 ml), Liquid DAB chromogen (10 ml) QD630-XAKE 139 IHC Detection Systems III. Super Sensitive Link-Label IHC Detection Systems TM A classic system based on the highly specific and sensitive streptavidin-biotin interaction to detect a bound antibody. The immunohistochemical reaction takes place in following steps: 1. Cells or tissues are prepared and then incubated with an unlabeled primary antibody that will bind to the antigen. 2. The bound antibody is detected with a species-specific secondary antibody that will recognize a group of primary antibodies (e.g. primary antibodies made in rabbits). The secondary antibody is labeled with multiple biotin residues. 3. The bound secondary antibody is then allowed to react with Streptavidin conjugated with an enzyme. Streptavidin binds extremely strongly and irreversibly to the biotin residues on the secondary antibody resulting in the addition of multiple enzyme to the primary antibody complex. 4. A substrate is then added and acted upon by the enzyme producing a highly visible precipitate. Benefits • Improved staining of weak antigens • User-friendly and extensive choice of kit formats designed for use with human, animal or rodent tissue • Wide choice of chromogens offered Super SensitiveTM Link-Label IHC Detection System is available in manual and automated formats. 140 www.biogenex.com IHC Detection Systems IIIA. Manual Use Product Type Ready to-Use Link MultiLink ® Label Chromogen Pack size (Slides) Cat. No. HRP AEC 60 QP000-5L 60 QD470-60K Contents: Link (6 mL Biotinylated Anti-Immunoglobulins), Chromogen (AEC Liquid,) Negative Control (3 mL serum) Label (6mL Enzyme-Conjugated Streptavidin), Substrate, Peroxide Block (6 mL) Ready to use MultiLink® HRP DAB/2 component Contents: Link (6 mL Biotinylated Anti-immunoglobulins), Chromogen 2 mL (DAB) Negative Control (3 mL x 2 serum) Label (6 mL Enzyme-Conjugated Streptavidin), Peroxide block (6 mL), Hematoxylin (6 mL) Large Volume MultiLink® Alkaline Phosphatase 500 QA900-9L MultiLink® HRP 500 QP900-9L HRP 1000 QP300-XAK MultiLink® Alkaline Phosphatase 1000+ LA000-ULE MultiLink® HRP 1000+ LP000-ULE Contents: Link (50 mL Biotinylated Anti-immunoglobulins) Label (50 ml Enzyme-Conjugated Streptavidin) Mega Volume MultiLink® Contents: Link (100 mL Biotinylated Anti-Immunoglobulins) Label (100 mL Enzyme-Conjugated Streptavidin) Concentrated Contents: Link (5 mL Concentrated Biotinylated Anti-Immunoglobulins), Label (5 mL Concentrated Enzyme-Conjugated Streptavidin), Dilution Vials (2 each) internationalcs@biogenex.com 141 IHC Detection Systems Links and Labels Ready-to-use Links Links are available as either species-specific or MultiLink® secondary antibodies. MultiLink® permits detection of mouse IgG+IgM and rabbit Ig primary antibodies, providing a universal link reagent for use with most monoclonal and polyclonal antibodies. Links - Super SensitiveTM Systems Product Name Pack size Cat. No. SS MultiLink® (Biotinylated Goat Anti-Immunoglobulins, for use with Mouse and Rabbit Primary Antibodies) 6 ml 50 ml HK340-5K* HK340-9K* SS Mouse Link (Biotinylated Goat Anti-mouse Immunoglobulins) 6 ml 50 ml HK335-5M HK335-9M SS Rabbit Link (Biotinylated Goat Anti-rabbit Immunoglobulins) 6 ml 50 ml HK336-5R HK336-9R SS Goat Link (Biotinylated Rabbit Anti-goat Immunoglobulins) 6 ml HK337-5G SS Rat Link (Biotinylated Rabbit Anti-rat Immunoglobulins) 6 ml HK338-5T Ready-to-use Labels RTU labels for biotin-streptavidin detection systems are compatible with the Super SensitiveTM Links. Labels include horseradish peroxidase and alkaline phosphatase conjugates. Labels - Super SensitiveTM Systems Product Name Pack size Cat. No. SS HRP Label (Peroxidase-Conjugated Streptavidin) 6 ml 50 ml HK330-5K HK330-9K SS AP Label (Alkaline Phosphatase-Conjugated Streptavidin) 6 ml 50 ml HK331-5K HK331-9K Concentrated Link The concentrated links are available in MultiLink® formats, and are supplied with recommended dilutions for use with BioGenex Antibodies. MultiLink® provides a universal link reagent for use with most monoclonal and polyclonal antibodies. Link Diluent (Cat. No. HK165-5K) should be used to dilute all concentrated links. MultiLink® Product Name Pack size Cat. No. Concentrated MultiLink® (Biotinylated Anti-immunoglobulins, for use with Mouse, Guinea Pig, Rat and Rabbit Primary Antibodies) 5 ml HK268-UK Concentrated Mouse Link (Biotinylated Anti-mouse Immunoglobulins) 5 ml HK325-UM Concentrated Rabbit Link (Biotinylated Anti-rabbit Immunoglobulins) 5 ml HK326-UR Concentrated SS Goat Link (Biotinylated Rabbit Anti-goat Immunoglobulins) 5 ml HK327-UG Concentrated Label Concentrated Labels are supplied with recommended dilutions for use with BioGenex antibodies. Concentrated peroxidase labels are recommended to be diluted in Streptavidin Peroxidase Diluent (Cat. No. HK157-5K). HRP Label Product Name Pack size Cat. No. Concentrated HRP Label (Peroxidase-Conjugated Streptavidin) 5 ml HK320-UK Concentrated AP Label (Alkaline Phosphatase-Conjugated Streptavidin) 5 ml HK321-UK 142 www.biogenex.com Multi-Staining Multi-Staining Double Staining BioGenex Double Staining IHC products include pre-optimized antibody cocktails and Super Sensitive multiple detection systems, enabling simultaneous testing of multiple antigens on single slide with a fast and easy protocol, assisting rapid and accurate diagnosis. Super Sensitive™ Double Staining Polymer Detection System This double staining system is designed with novel polymer technology for fast and easy IHC staining of multiple antigens on a single slide. This system is pre-optimized for human tissues with superior sensitivity and specificity to produce precise and reliable results that allow easy interpretation and accurate diagnosis. • Pre-mixed and pre-optimized polymer cocktails • More patient data per slide – testing multiple protein biomarkers simultaneously • Easy and fast – staining with a 4-step protocol • Saving costs by maximizing resources • Clean and intense stain without endogenous biotin background Product Name Pack Size Contents Cat. No. 60 Slides 6ml Anti-Rabbit Polymer-HRP + Anti-Mouse Polymer-AP Cocktail, 12ml Peroxide Block, 12ml Power BlockTM, 6ml Mouse Negative control, 6ml Rabbit Negative Control, 4ml Liquid DAB chromogen, 10ml Stable DAB Substrate Buffer, 20 ml Permanent Fast Red A, 20ml Permanent Fast Red B QS200-60K 60 Slides 6ml Anti-Rabbit Polymer-AP + Anti-Mouse Polymer-HRP Cocktail, 12ml Peroxide Block, 12ml Power BlockTM, 6ml Mouse Negative Control, 6ml Rabbit Negative Control, 4ml Liquid DAB chromogen, 10ml Stable DAB Substrate Buffer, 20ml Permanent Fast Red A, 20ml Permanent Fast Red B QS400-60K Ready-to-use Detection System Super Sensitive™ Double Staining Polymer Detection Kit I / DAB & Permanent Fast Red Super Sensitive™ Double Staining Polymer Detection Kit II / DAB & Permanent Fast Red Ready-to-use Detection System (Large Volume) Super Sensitive™ Double Staining Polymer Detection Kit I Large Volume 500 Slides 50ml Anti-Rabbit Polymer-HRP + Anti-Mouse Polymer-AP Cocktail QS210-YIK Super Sensitive™ Double Staining Polymer Detection Kit II Large Volume 500 Slides 50ml Anti-Rabbit Polymer-AP + Anti-Mouse Polymer-HRP Cocktail QS410-YIK 144 www.biogenex.com Multi-Staining Double Staining Super Sensitive™ Antibody Cocktails • • • • • Pre-mixed and pre-optimized antibody cocktails More patient data per slide – testing multiple protein biomarkers simultaneously Easy and fast – staining with a 4-step protocol Saving costs by maximizing resources Excellent sensitivity and high antibody efficiency CD10 + Cyclin D1 CD4 + CD8 Source & Clone: Mouse 56C6 + Rabbit EPR2241(IHC)-32 Isotype: IgG1 + IgG Localization: Membrane, Nucleus Pre-treatment: EZ-ARTM 1, EZ-AR 2 Manual: HK521-XAK Xmatrx: HX031-YCD/HX032-YCD Source & Clone: Mouse 4B12 + Rabbit EP1150Y Isotype: IgG1 + IgG Localization: Membrane Pre-treatment: AR-10/ EZ-AR 1, EZ-AR 2 Manual/i6000TM: HK058-5K Xmatrx: HX031-YCD/HX032-YCD Tonsil stained with anti-CD4 + CD8 Breast carcinoma stained with antiCD10+Cyclin D1 Ready-to-use (Manual): AC561-5M Ready-to-use (Automated) i6000™: Xmatrx®: AC561-10M AC561-YCD Recommended Positive Control: Breast carcinoma Ready-to-use (Manual): AC595-5M Ready-to-use (Automated) i6000™: Xmatrx®: AC595-10M AC595-YCD CD10, also known as common acute lymphoblastic leukemia antigen (CALLA), is a cell surface enzyme which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt’s, and follicular germinal center lymphomas and in chronic myelogenous leukemia (CML). Cyclins are a family of protein that act as key regulators of the cell cycle. Cyclin D1 (CCND1) is involved in the G1/S transition of the cell cycle by activating Cdk4 and Cdk6 and inhibiting the retinoblastoma protein. Overexpression of Cyclin D1 has been associated with the development of many cancers including breast cancer, colon cancer, prostate cancer, and mantle cell lymphoma. Cyclin D1 and CD10 are useful in the differential diagnosis and confirmation of mantle cell lymphoma. Tonsil adenocarcinoma CD4 is a single chain transmembrane glycoprotein expressed on the surface of T helper cells and regulatory T-cells. CD4 is a co-receptor that assists the T-cell receptor (TCR) with an antigen-presenting cell. CD4 interacts directly with MHC class II molecules on the surface of the antigen-presenting cell. CD8 is a transmembrane glycoprotein that serves as a co-receptor for the T-cell receptor (TCR). The CD8 coreceptor is predominantly expressed on the surface of cytotoxic T-cells, but can also be found on natural killer cells and dendritic cells. CD8 binds to a major histocompatibility complex (MHC) molecule, but is specific for the class I MHC protein. CD4-CD8 double staining reveals the distribution of T-lymphocyte subsets, for example in HIV infection, infiltrating cells in graft rejection and lymphoma. CD3 + CD20 CDX-2 + CK7 Recommended Positive Control: Source & Clone: Rabbit EP449E + Mouse L-26 Source & Clone: Mouse CDX-2-88 + Rabbit EPR1619Y Isotype: IgG + IgG2a Kappa Isotype: Membrane & Cytoplasm, Membrane Localization: Nucleus, Cytoplasm Pre-treatment: AR Citra Plus/EZ-AR 1, EZ-AR 2 Localization: IgG1 Kappa + IgG Pre-treatment: EZ-AR TM 1, EZ-AR 2 Manual: HK521-XAK Manual/i6000TM: HK081-5K HX031-YCD/HX032-YCD Xmatrx: Xmatrx: CDX2 and CK7 stained in colon cancer metastasized into lung tissue Tonsil stained with Anti-CD3 and Anti-CD20 Ready-to-use (Manual): AC559-5M Ready-to-use (Automated) i6000™: Xmatrx®: AC559-10M AC559-YCD Recommended Positive Control: Tonsil or spleen CD3 is a lineage-specific “pan T-Cell” surface antigen compose of five different polypeptide chains. The CD3 antibody recognizes the human CD3 antigen which is present on mature human T-cells, thymocytes, and a subset of NK cells. CD20 is a non-glycosylated phosphoprotein expressed on the surface of all mature B-cells. The CD20 antibody primarily recognizes a 33 kD polypepitide B-cell component and a mono 30 kD cellular antigen. The CD3-CD20 cocktail reveals lymphoid architecture in neoplastic situations. CD20 and CD3 antibodies are provided at optimal concentrations in an easy-to-use format for visualizing T- and B-lymphocytes. This antibody cocktails can be used for the typing of malignant lymphomas and other lymphoproliferative disorders. internationalcs@biogenex.com HX031-YCD/HX032-YCD Ready-to-use (Manual): AC596-5M Ready-to-use (Automated) i6000™: Xmatrx®: Recommended Positive Control: AC596-10M AC596-YCD Colon or breast carcinoma CDX-2, a member of the caudal-related homeobox family, is an intestine-specific transcription factor that regulates both proliferation and differentiation in intestinal epithelial cells. It plays an important role in triggering cells toward the phenotype of differentiated villus enterocytes as well as in the maintenance of the phenotype. CDX2 is used as a marker for gastrointestinal differentiation, especially colorectal. Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. CDX-2 and CK7 combination will help to distinguish the tumor origin from gastrointestinal, especially colorectal to adenocarcinomas of lung, breast, endometrioid tumors, and transitional cell carcinoma of the bladder. 145 Multi-Staining Double Staining Ki67 + Lambda TTF-1 + CK5&6 Source & Clone: Mouse 8G7G3/1+ Rabbit EPR1600Y+ Rabbit EPR1602Y Source & Clone: Mouse K-2 + Rabbit polyclonal Isotype: IgG + Polyclonal Localization: Nucleus, Cytoplasm Pre-treatment: AR Citra Plus/ EZ-AR 1, EZ-AR 2 Manual/i6000TM: HK081-5K Xmatrx: Isotype: TTF-1 IgG1 Kappa + CK5&6 IgG Localization: TTF-1 Nucleus, CK5&6 Cytoplasm Pre-treatment: AR Citra Plus/ EZ-AR 1, EZ-AR 2 HX031-YCD/HX032-YCD Tonsil stained with anti-Ki67 + Lambda Lung squamous cell carcinoma stained with anti-TTF1+CK5&6 Ready-to-use (Manual): AC562-5M Ready-to-use (Automated) i6000™: Xmatrx®: AC562-10M AC562-YCD Recommended Positive Control: Tonsil Ki67 is a nuclear protein present in cells at all phase of the cell cycle except G0. As such, Ki67 is a useful marker to identify the proliferation activity of cell populations. The staining of this activity, designated as the Ki67 labeling index, has shown to be clinically significant as a prognosis marker for breast, colorectal, skin cancer, and various lymphomas. The light chain is a polypeptide subunit of immunoglobulin expressed by B-cells. These B-cells are restricted to one of two subtypes of light chain, lambda or kappa. As a result, the light chain is a useful marker for lymphomas characterized as a monoclonal proliferation of B-cells. The Ki67 and lambda light chain cocktail is useful in evaluating cell proliferation of lambda light chain positive tumors. p16 + Ki67 Manual/i6000TM: HK081-5K Xmatrx: HX031-YCD/HX032-YCD Ready-to-use (Manual): AC563-5M Ready-to-use (Automated) i6000™: Xmatrx®: AC563-10M AC563-YCD Recommended Positive Control: Lung squamous carcinoma Thyroid transcription factor 1 (TTF-1) is a homeodomain-containing transcription factor that regulates the expression of thyroid and lung-specific genes. TTF-1 is detected in small cell carcinomas, neuroendocrine carcinomas, and thyroid carcinomas. As such, TTF1 can help identify primary pulmonary carcinomas and differentiate it from metastatic carcinomas. Keratins are intermediate filaments found in the cytoskeleton of epithelial cells. Cytokeratin (CK) 5 and 6 are both type II keratins and are associated with the differentiation of simple and stratified epithelial tissue. CK 5/6 has been documented as a useful positive marker for diagnosing malignant mesothelioma. Together, TTF1 and CK 5 /6 antibodies form valuable markers to help differentiate between mesotheliomas and pulmonary adenocarcinomas. TTF-1 + GCDFP-15 Source & Clone: Mouse G175-405 + Rabbit EPR3611 Isotype: IgG + IgG Source & Clone: Mouse 8G7G3/1+Rabbit EP1582Y Localization: Nucleus and/or Cytoplasm, Nucleus Isotype: Pre-treatment: AR Citra Plus Manual/i6000TM: HK081-5K IgG1 Kappa + IgG Localization: Nucleus, Cytoplasm Pre-treatment: AR Citra/ EZ-AR 1, EZ-AR 2 Manual/i6000TM: HK087-5K Xmatrx: HX031-YCD/HX032-YCD Cervical carcinoma stained with Anti-p16+Ki67 Ready-to-use (Manual): Lung squamous cell carcinoma stained with Anti-TTF1 +GCDFP-15 AC601-5M Ready-to-use (Automated) i6000™: AC601-10M Recommended Positive Control: Cervical adenocarcinoma RU O p16/INK4A is a tumor-suppressor protein. The related genetic and epigenetic abnormalities in genes controlling the G1 checkpoint can lead to both escape from senescence and cancer formation. Ki-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. p16/Ki-67 immunostains are helpful to assess cervical biopsies for HPV-associated lesions. For research use only, not for use in diagnostic procedures. 146 Ready-to-use (Manual): AC604-5M Ready-to-use (Automated) i6000™: Xmatrx®: AC604-10M AC604-YCD Recommended Positive Control: Lung squamous carcinoma Thyroid transcription factor-1 (TTF-1) is a sensitive marker for diagnosis of primary pulmonary adenocarcinoma, and differentiation between poorly differentiated squamous cell carcinoma and small cell carcinoma and adenocarcinoma. Gross cystic disease fluid protein (GCDFP-15) is currently used as an immunohistochemical marker of breast cancer. TTF-1/GCDFP-15 immunohistochemical profile in lung tumors is highly suggestive of metastatic carcinoma of the breast. In distinguishing metastatic breast carcinoma and adenocarcinoma of the lung, the cytoplasmic staining would indicate breast carcinoma and nuclear staining would indicate lung or thyroid carcinoma. www.biogenex.com Multi-Staining Double Staining CDX-2 + CK20 + CK7 PIN4 (p63 + CK HMW + p504S) Source & Clone: Mouse CDX-2-88 + Rabbit EPR1622Y + Rabbit EPR1619Y Isotype: IgG1 Kappa + IgG + IgG Localization: CDX-2 Nucleus, CK20 Cytoplasm, CK7 Cytoplasm Manual/i6000TM: HK081-5K Xmatrx: HX031-YCD/HX032-YCD Ready-to-use (Manual): Ready-to-use (Automated) i6000™: Xmatrx®: AC597-5M AC597-10M AC597-YCD Recommended Positive Control: Colon or breast carcinoma CDX-2, a member of the caudal-related homeobox family, is an intestine-specific transcription factor that regulates both proliferation and differentiation in intestinal epithelial cells. CDX-2 is used as a marker for gastrointestinal differentiation, especially colorectal. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. CK7 expression is significantly more frequent in adenocarcinomas of pulmonary and breast origin than gastrointestinal (GI) origin, In contrast, CK20 expression is significantly more prevalent in gastrointestinal adenocarcinoma than that of pulmonary or breast origin. CDX-2/CK20/CK7 combination will help to distinguish the tumor origin from gastrointestinal, especially colorectal to adenocarcinomas of lung, breast, endometrioid tumors. Isotype: IgG Localization: p63 Nucleus, CK HMW and p504S Cytoplasm Pre-treatment: AR Citra Plus/EZ-AR 2 Manual/i6000TM: HK081-5K Xmatrx: AR Citra Plus/ EZ-AR 1, EZ-AR 2 Pre-treatment: Colon carcinoma stained with AntiCDX-2+CK20+CK7 Source & Clone: Mouse 4A4 + Mouse 34ßE12 + Rabbit 13H4 HX032-YCDE Prostate carcinoma. stained with anti-PIN4 Ready-to-use (Manual): AM448-5ME Ready-to-use (Automated) i6000™: Xmatrx®: AM448-10ME AX448-YCDE Recommended Positive Control: Prostate adenocarcinoma Concentrated: MU448-UCE RU O This antibody cocktail recognizes Prostate Intraepithelial Neoplasia (PIN) in the tissues stained by immunohistochemical techniques. A cocktail of these three antibodies might allow simultaneous demonstration of P504S, HMW CK and p63 using a single immunostain. The combination of P504S + HMW CK + p63 (PIN4 Cocktail) may be extremely useful for studying prostatic intraepithelial neoplasia, especially in difficult cases and in cases with limited tissue. For Research Use only, not for use in diagnostic procedures. For research use only. Not for use in diagnostic procedures. TTF-1 + CK20 + CK7 p63 + CK5 Source & Clone: Mouse 8G7G3/1+ Rabbit EPR1622Y + Rabbit EPR1619Y Source & Clone: Mouse 4A4 + Rabbit EPR1600Y Isotype: IgG Localization: Nucleus, Cytoplasm Pre-treatment: AR Citra Plus/ EZ-AR 1, EZ-AR 2 Isotype: IgG1 Kappa + IgG + IgG Localization: TTF-1 Nucleus, CK20 Cytoplasm, CK7 Cytoplasm Pre-treatment: AR Citra Plus/ EZ-AR 1, EZ-AR 2 Manual/i6000TM: HK081-5K Xmatrx: HX031-YCDE HX032-YCDE Prostate tissue stained with Antip63+CK5 Ready-to-use (Manual): AC602-5M Ready-to-use (Automated) i6000™: Xmatrx®: AC602-10M AC602-YCD Recommended Positive Control: Prostate adenocarcinoma Tumor protein p63 is a member of the p53 family of transcription factors. Cytokeratin 5 (58 kD) is expressed in basal, intermediate and superficial cell layers of stratified epithelia as well as transitional epithelia, and in mesothelial cells and mesothelioma. Cytokeratin 6 (56 kD) is also a high molecular weight cytokeratin expressed by proliferating squamous epithelium. p63 and cytokeratin (CK 5/6) are markers of basal and squamous differentiation in several normal epithelia and human tumors. p63 and cytokeratin (CK 5/6) are also helpful in distinguishing poorly differentiated squamous cell carcinoma from small cell carcinoma or adenocarcinoma. Simultaneous detection of p63, CK5/6 can help increase the diagnostic accuracy of breast diseases. internationalcs@biogenex.com Lung/Breast carcinoma stained with Anti-TTF-1+CK20+CK7 Manual/i6000TM: HK081-5K Xmatrx: Ready-to-use (Manual): Ready-to-use (Automated) i6000™: Xmatrx®: Recommended Positive Control: HX031-YCD/HX032-YCD AC605-5M AC605-10M AC605-YCD Lung carcinoma Thyroid transcription factor-1 (TTF-1) is a sensitive marker for the diagnosis of primary pulmonary adenocarcinoma, and differentiation between poorly differentiated squamous cell carcinoma and small cell carcinoma and adenocarcinoma. CK7 expression is significantly more frequent in adenocarcinomas of pulmonary and breast origin than gastrointestinal (GI) origin, In contrast, CK20 expression is significantly more prevalent in gastrointestinal adenocarcinoma than that of pulmonary or breast origin. TTF-1/CK20/CK7 is a useful marker for diagnosis of lung tumors and for differential diagnosis of primary pulmonary adenocarcinomas from extrapulmonary adenocarcinomas metastatic to the lung. Application of this panel of antibodies might increase the accuracy of diagnosis. 147 Multi-Staining 148 www.biogenex.com ISH Probes & Detection Systems ISH Probes & Detection Systems In situ Hybridization Probes BioGenex offers fluorescein-labeled oligonucleotide probes for the detection of RNA or DNA by in situ Hybridization (ISH). These probes allow the localization of specific nucleic acid sequences within cells from formalin-fixed, paraffin-embedded tissue sections. When used with the BioGenex ISH Detection systems, these probes offer reliable, highly sensitive and easy-to-perform DNA and RNA assays. CerviPro HPV Type 16/18 DNA Probe Alu II Probe Ready-to-use (Manual): PR026-100E(ASR) Ready-to-use (Manual): PR250-100E (ASR) Specificity: Alu II DNA Specificity: HPV viral DNA sequences Ready-to-use (Automated): Ready-to-use (Automated): Xmatrx: PR026-YADE (RUO) Xmatrx: PR250-YADE (RUO) Alu sequence detected in FFPE tissue stained with DAB HPV16/18 in Ca Cervix stained with DAB Alu, an important group of widely distributed sequences repeated in the human genome, has been widely used in in situ hybridization technique. Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only. The CerviPro HPV Type 16/18 DNA probe has been designed to recognize regions of the E1, E6, L1, and L2 open reading frames (ORFs) of human papillomavirus (HPV) genotypes in paraffin embedded human tissues or cytopathology specimens/cervical scraps. Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only. Beta-Actin* Ready-to-use (Manual): PR1055-100E (ASR) Specificity: Beta-Actin RNA Cyclin D1 Probe* Ready-to-use Automated): Xmatrx: PR1055-YADE (RUO) Ready-to-use (Manual): PR216-100E (ASR) Specificity: Cyclin D1 RNA Ready-to-use (Automated): Xmatrx: PR216-YADE (RUO) Beta actin mRNA staining of breast cancer tissue Actins are highly conserved proteins that participate in cell motility as well as cell structure and integrity. In normal cells, beta-actin mRNA is localized in cell protrusions where actin is actively polymerized. Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only. CerviPro HPV 14 DNA Probe Ready-to-use (Manual): PR251-100E (ASR) Specificity: HPV 14 genotypes Cyclin D1 mRNA in human Lymphoma tissue stained with DAB Cyclin D1, also known as PARD-1 or bcl1, is a G1 phase cyclin, which regulates G1 phase progression and G1/S transition of the cell cycle. Cyclin D1 mRNA is overexpressed in many cancer types including colon cancer, gastric cancer and breast cancer. Its overexpression is correlated with poor prognosis. Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only. EBV-Encoded RNA (EBER) Probe Ready-to-use (Automated): Xmatrx: PR251-YADE (RUO) Ready-to-use (Manual): PR205-100E (ASR) Specificity: EBV-encoded RNA Ready-to-use Automated): Xmatrx: PR205-YADE (RUO) HPV 14 HR genotype in Ca Cervix tissue stained with DAB The HPV 14 probe has been designed to specifically recognize regions of the L1 and E6/E7 open reading frames (ORFs) of human papillomavirus (HPV) 14 genotypes (HPV 16,18,31,33,35,39,45,51,52,56,58,59,66,68) in paraffin embedded human tissues or cytopathology specimens/ cervical scraps. Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only. * Epstein-Barr early RNA (EBER) stained with DAB Epstein-Barr virus-encoded RNA, EBER, is present in cells latently infected with Epstein-Barr virus (EBV). Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only. To be released soon 150 www.biogenex.com ISH Probes & Detection Systems Kappa Probe Oligo d (T) Probe* Ready-to-use (Manual): PR214-100E (ASR) Ready-to-use (Manual): PR217-100E (ASR) Specificity: Kappa light chain mRNA Specificity: mRNA Ready-to-use (Automated): Ready-to-use (Automated): Xmatrx: PR217-YADE (RUO) Xmatrx: PR214-YADE (RUO) Human immunoglobulin kappa light chain mRNA in tonsil stained with DAB Preservation of oligo d (T) mRNA in FFPE tissue stained with DAB The light chains of immunoglobulin molecules have two antigenic types: kappa and lambda. A given immunoglobulin molecule contains two identical light chains, either kappa or lambda. Therefore, the clonal nature of any immunoglobulin producing cell population can be determined by the light chain structure of the immunoglobulin that the cell produces. Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only. Lambda Probe Ready-to-use (Manual): PR215-100E (ASR) Specificity: Lambda light chain mRNA In all living cells, the expression of genetic information involves transcription of RNA molecules. The initial transcripts named heterogeneous nuclear RNA (hnRNA) are processed into mature messenger RNA (mRNA) by removing non-coding intron sequences and adding the 5’-methyl cap and a 3’-tail of approximately 200 adenylyl residues (poly (A)). In general, mRNA are conserved in routine formalin-fixed, paraffin-embedded tissues which have been fixed promptly. However, mRNA is not stable and may be destroyed during tissue processing of a routine formalin-fixed, paraffin-embedded tissue specimen. in situ hybridization with an oligo-d (T) probe is commonly used to assess the preservation of mRNA in a formalin-fixed, paraffin embedded tissue specimen. Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only. Ready-to-use (Automated): Xmatrx: PR215-YADE (RUO) Retinoblastoma (RB) Probe* Human immunoglobulin lambda light chain mRNA in tonsil stained with DAB Ready-to-use (Manual): PR225-100E (ASR) Specificity: retinoblastoma tumor suppressor gene Ready-to-use (Automated): Xmatrx: PR225-YADE (RUO) The light chains of immunoglobulin molecules have two antigenic types: kappa and lambda. A given immunoglobulin molecule contains two identical light chains, either kappa or lambda. Therefore, the clonal nature of any immunoglobulin producing cell population can be determined by the light chain structure of the immunoglobulin that the cell produces. Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only. ISH Probes Retinoblastoma mRNA in Adenocarcinoma tissue stained with DAB The retinoblastoma tumor suppressor gene, RB, encodes a protein of 110 KD that plays an important role in cell growth regulation. Alterations in Retinoblastoma (RB1) mRNA expression have been reported in many human tumor types including lung cancer, osteosarcomas, leukemias, prostate cancer and bladder cancer. Increased expression of RB1 mRNA has been reported for many human colon tumor tissues and human colorectal cancer cell lines and Breast cancer. Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only. Probes are packaged with RFID vials for staining up to 100 slides. * Product Name Pack size Intended Use Cat. No. (Manual) Cat. No. (Automated) Alu II Probe 25 slides Alu II sequences PR026-100E PR026-YADE Beta Actin* 25 slides Initial standard PR1055-100E PR1055-YADE CerviPro HPV 14 25 slides L1 and E6/E7 ORFs of HPV14 PR251-100E PR251-YADE CerviPro HPV Type 16/18 25 slides E1, E6, L1, and L2 open reading frames (ORFs) of HPV PR250-100E PR250-YADE Cyclin D1* 25 slides Cyclin D1, a G1 phase cyclin PR216-100E PR216-YADE EBER Probe 25 slides EBV-encoded RNA PR205-100E PR205-YADE Kappa Probe 25 slides Kappa light chain mRNA PR214-100E PR214-YADE Lamda Probe 25 slides Lambda light chain mRNA PR215-100E PR215-YADE Oligo d (T) Probe* 25 slides mRNA PR217-100E PR217-YADE Retinoblastoma (RB) Probe* 25 slides Retinoblastoma tumor suppressor gene PR225-100E PR255-YADE To be released soon internationalcs@biogenex.com 151 ISH Probes & Detection Systems MicroRNA Probes MicroRNAs (miRNAs) are endogenous, non-coding RNAs known to regulate gene expression by translational repression or RNA cleavage. Since miRNA has been observed to deregulate during progression of different cancer stages from normal to malignant and metastasis, the expression proile as a result of this deregulation can be exploited as a potential biomarker for cancer characterization. BioGenex MicroRNA Probes Automated Protocols • Optimized for automated ISH staining by Xmatrx ELITE • Ready-to-use reagents for FFPE tissues Highly Speciic and Sensitive Probes • Proprietary technology for clean intense stains • in situ context of tissue morphology Manual Protocols • Optimized for standardized manual ISH staining • Ready-to-use reagents for FFPE tissues Hsa-miR-1 Hsa-miR-17 Ready-to-use (Manual): HM001-100E Ready-to-use (Manual): HM017-100E Specificity: miR-1 Specificity: miR-17 Hsa-miR-1 detected in FFPE tissue stained with DAB Hsa-miR-17 detected in FFPE tissue stained with DAB The Hsa-miR-1probe has been designed from mature human miR-1 sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. The Hsa-miR-17 probe has been designed from mature human miR-17 sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. Hsa-miR-10b Hsa-miR-200a Ready-to-use (Manual): HM010B-100E Ready-to-use (Manual): HM200a-100E Specificity: miR-10b Specificity: miR-200a Hsa-miR-10b detected in FFPE tissue stained with DAB Hsa-miR-200a detected in FFPE tissue stained with DAB The Hsa-miR-10b probe has been designed from mature human miR-10b sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. The Hsa-miR-200a probe has been designed from mature human miR-200a sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. 152 www.biogenex.com ISH Probes & Detection Systems Hsa-miR-21 Hsa-miR-126 Ready-to-use (Manual): HM021-100E Ready-to-use (Manual): HM126-100E Specificity: miR-21 Specificity: miR126 Hsa-miR 21 detected in FFPE tissue stained with DAB Hsa-miR-126 detected in FFPE tissue stained with DAB The Hsa-miR-21 probe has been designed from mature human miR-21 sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. The Hsa-miR-126 probe has been designed from mature human miR-126 sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. Hsa-miR-106a Hsa-miR-141 Ready-to-use (Manual): HM106A-100E Ready-to-use (Manual): HM141-100E Specificity: miR-106a Specificity: miR141 Hsa-miR-106a detected in FFPE tissue stained with DAB Hsa-miR-141 detected in FFPE tissue stained with DAB The Hsa-miR-106a probe has been designed from mature human miR-106a sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. The Hsa-miR-141 probe has been designed from mature human miR-141 sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. Hsa-miR-125b Hsa-miR-144 Ready-to-use (Manual): HM125B-100E Ready-to-use (Manual): HM144-100E Specificity: miR-125b Specificity: miR-144 Hsa-miR-125b detected in FFPE tissue stained with DAB Hsa-miR-144 detected in FFPE tissue stained with DAB The Hsa-miR-125bprobe has been designed from mature human miR-125b sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. The Hsa-miR-144 probe has been designed from mature human miR-144 sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. internationalcs@biogenex.com 153 ISH Probes & Detection Systems Hsa-miR-146a Hsa-miR-151a-3p Ready-to-use (Manual): HM146A-100E Ready-to-use (Manual): HM151-100E Specificity: miR-146a Specificity: miR151a-3p Hsa-miR 146 detected in FFPE tissue stained with DAB Hsa-miR-151a-3p detected in FFPE tissue stained with DAB The Hsa-miR-146a probe has been designed from mature human miR-146a sequence. This fluorescenated probe isprovided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. Hsa-miR-147b Hsa-miR-152 Ready-to-use (Manual): HM147B-100E Specificity: miR147b Hsa-miR147b detected in FFPE tissue stained with DAB Ready-to-use (Manual): HM152-100E Specificity: miR152 Hsa-miR-152 detected in FFPE tissue stained with DAB The Hsa-miR-147b probe has been designed from mature human miR-147b sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. Hsa-miR-150 The Hsa-miR-152 probe has been designed from mature human miR-152 sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. Hsa-miR-155 Ready-to-use (Manual): HM150-100E Specificity: miR-150 Hsa-miR 150 detected in FFPE tissue stained with DAB The Hsa-miR-150 probe has been designed from mature human miR-150 sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. 154 The Hsa-miR-151a-3p probe has been designed from mature human miR-10b sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. Ready-to-use (Manual): HM155-100E Specificity: miR-155 Has-miR 155 detected in FFPE tissue stained with DAB The Hsa-miR-155 probe has been designed from mature human miR-155 sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. www.biogenex.com ISH Probes & Detection Systems Hsa-miR-205 Hsa-miR-196a Ready-to-use (Manual): HM196A-100E Ready-to-use (Manual): HM205-100E Specificity: miR-196a Specificity: miR-205 Hsa-miR-196a detected in FFPE tissue stained with DAB Hsa-miR-205 detected in FFPE tissue stained with DAB The Hsa-miR-196a probe has been designed from mature human miR-196a sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. The Hsa-miR-205 probe has been designed from mature human miR-205 sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. Hsa-miR-200b Hsa-miR-216a Ready-to-use (Manual): HM200B-100E Ready-to-use (Manual): HM216A-100E Specificity: miR-200b Specificity: mIr-216a Hsa-miR-200b detected in FFPE tissue stained with DAB Hsa-miR-216a detected in FFPE tissue stained with DAB The Hsa-miR-200b probe has been designed from mature human miR-200b sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. The Hsa-miR-216a probe has been designed from mature human miR-216a sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. Hsa-miR-204 Hsa-miR-222 Ready-to-use (Manual): HM204-100E Ready-to-use (Manual): HM222-100E Specificity: miR-204 Specificity: miR-222 Hsa-miR-204 detected in FFPE tissue stained with DAB Hsa-miR 222 detected in FFPE tissue stained with DAB The Hsa-miR-204 probe has been designed from mature human miR-204 sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. The Hsa-miR-222 probe has been designed from mature human miR-222 sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. internationalcs@biogenex.com 155 ISH Probes & Detection Systems Hsa-miR-328 Hsa-miR-let-7a Ready-to-use (Manual): HM328-100E Ready-to-use (Manual): HM007A-100E Specificity: miR-328 Specificity: LET-7a Hsa-miR 328 detected in FFPE tissue stained with DAB Hsa-miR-let-7a detected in FFPE tissue stained with DAB The Hsa-miR-328 probe has been designed from mature human miR-328 sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. The Hsa-let-7a probe has been designed from mature human let-7a sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. Hsa-miR-375 Scramble probe Ready-to-use (Manual): HM375-100E Specificity: miR375 Hsa-miR-375 detected in FFPE tissue stained with DAB Ready-to-use (Manual): PR032-100E Specificity: Negative control for ISH assays Negative staining of scrambled probe in FFPE tissue The Hsa-miR-375 probe has been designed from mature human miR-375 sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. The scramble probe sequence does not share homology with miRNA sequences available in the miRBase database. Scramble probe is used as a negative control during miRNA ISH. Analyte Specific Reagent. Analytical and performance characteristics are not established. U6 probe Hsa-miR-1285 Ready-to-use (Manual): HM1285-100E Ready-to-use (Manual) PR031-100E Specificity: miR1285 Specificity human U6 small nuclear RNA Hsa-miR1285 detected in FFPE tissue stained with DAB U6 detected in FFPE tissue stained with DAB The Hsa-miR-1285 probe has been designed from mature human miR-1285 sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established. U6 snRNA is the non-coding small nuclear RNA (snRNA) component of U6 snRNP (small nuclearribonucleoprotein). The U6 snRNA sequence is highly conserved and the function of the U6 snRNA has remainedcrucial and unchanged through evolution. The U6 cellular transcript is available in abundance with intranuclear distribution in cell/tissue. The U6 probe is designed to bind to human U6 small nuclear RNA. Analyte Specific Reagent. Analytical and performance characteristics are not established. 156 www.biogenex.com ISH Probes & Detection Systems miRNA Probes Product Name Cat. No. Hsa-miR-1 probe HM001-100E Hsa-miR-10b probe HM010B-100E Hsa-miR-17 probe HM017-100E Hsa-miR-21 probe HM021-100E Hsa-miR-106a probe HM106A-100E Hsa-miR-125b probe HM125B-100E Hsa-miR-126 probe HM126-100E Has-miR-141 probe HM141-100E Hsa-miR-144 probe HM144-100E Hsa-miR-146a probe HM146A-100E Hsa-miR-147b probe HM147B-100E Hsa-miR-150 probe HM150-100E Hsa-miR-151-3p probe HM151-100E Hsa-miR-152 probe HM152-100E Hsa-miR-155 probe HM155-100E Hsa-miR-196a probe HM196A-100E Hsa-miR-200a probe HM200A-100E Hsa-miR-200b probe HM200B-100E Hsa-miR-204 probe HM204-100E Hsa-miR-205 probe HM205-100E Hsa-miR-216a probe HM216A-100E Hsa-miR-222 probe HM222-100E Hsa-miR-328 probe HM328-100E Hsa-miR-375 probe HM375-100E Hsa-miR-1285 probe HM1285-100E Hsa-miR-let7a probe HM007A-100E Scramble probe PR032 -100E U6 probe PR031-100E internationalcs@biogenex.com 157 ISH Probes & Detection Systems Hybridization Detection System in situ Hybridization (ISH) is a powerful technique for detecting and localizing specific nucleic acid sequences within cells or tissues. This is achieved by the hybridization of a labeled probe to the specific RNA/DNA sequence within the cell and subsequent detection of the bound probe. Features • Enable the semi-quantification of mRNA expression and helps to determine the temporal and spatial patterns of gene expression in cells, tissue and whole animals • Used for detecting intracellular pathogens with a very high degree of sensitivity Super Sensitive Polymer HRP ISH Detection System TM This is a novel detection system using a non-biotin polymeric technology that makes use of two major components : Super Enhancer and a Poly-HRP reagent. As the system is not based on the Biotin-Avidin System, problems associated with endogenous biotin are completely eliminated. The technology allows excellent cell penetration ability for intense staining, compared with other polymer HRPs. Benefits • Clean Stain without endogenous biotin background • High signal to noise ratio for intense stain • Universal system for all fluorescein labeled probes Product Name Pack Size Probe Type Cat. No. Super Sensitive™ One Step Polymer-HRP ISH Detection System 50 Slides Fluorescein Labeled DF400-50KE 50 slides Fluorescein Labeled DF400-YADE 1 x 5 ml Power BlockTM, 1 x 5 ml Peroxide Block, 1 x 10 ml DAB Buffer, 1 x 2.5 ml Liquid DAB Chromogen, 1 x 3 ml One step Poly-HRP Reagent; 1 x 10 ml Hematoxylin; 1 x 5 ml Proteinase K; 1 x 3 ml Nucleic Acid Retrieval 1 Solution; 1 x 20 ml Wash Solution A;1 x 20ml Wash Solution B; 1 x 20 ml Wash Solution E; 1 x 0 ml Wash Solution F; 1 x 3 ml Anti-Flourescein Antibody XISH One Step Polymer-HRP ISH Detection System 1 x 10 ml Power Block, 1 x 10 ml Peroxide Block, 4 x 5 ml DAB Buffer, 1 x 3 ml Liquid DAB Chromogen, 1 x 3 ml One-step Poly-HHP Reagent; 1 x 10 ml Hematoxyline; 1 x 3 ml Proteinase K; 1 x 3 ml Nucleic Acid Retrieval Solution; 2 x 10 ml Wash Solution E; 2 x 10 ml Wash Solution E; 2 x 10 ml Wash Solution F; 1 x 3 ml Anti-Flourescein Antibody 158 www.biogenex.com eFISHiency eFISHiency eFISHiency Fluorescence in situ hybridization (FISH) is a robust technique of cytogenetic used for the detection of chromosomal aberrations viz., deletions, amplification and translocation in tissue sections or within individual cells in native context. In this technique florescent probes bind to the target sequence of DNA chromosome. High specificity and sensitivity coupled with rapid and accurate result has proven the role of FISH in both research and diagnosis of solid tumor and hematological malignancies. FISH is also used in genetic counseling, medicine and species identification. FISH can also be used to detect and localize specific RNA targets in cells, circulating tumor cells and tissue samples. In an FISH procedure, fixed tissue sections/cytology specimens are pretreated to expose target DNA or mRNA sequences. An appropriately labeled probe is hybridized to the exposed target in the cells, followed by stringency washing steps to remove non-specifically bound probe. Subsequently slides are mounted using DAPI/antifade and can be visualized under fluorescence microscope using appropriate filter set. eFISHiency: Comprehensive high-throughput automated FISH processing systems BioGenex offers eFISHiency system, a complete solution for cytogenetic FISH laboratory requirements under one umbrella, consisting of eFISH probes, pretreatment kits and high-throughput automated/semi-automated platforms. Sr # 1 2 eFISHiency Components eFISH probes More than 50 FISH probes covering Probes for detection and diagnosis of genetic aberrations major genetic aberrations eFISH kits Description eFISH Histo eFISH kit for histology FFPE tissue samples eFISH Cyto eFISH kit for cytology specimens eFISH U eFISH kit for manual usage - both histology and cytology specimens. World’s only high-throughput front end FISH processing system that process FISH slides from microtome to microscope including final coverslipping. Xmatrx® ELITE 40 different protocols in combination of histology and cytology specimens/ probes can be processed at a time. 3 eFISH processing systems Xmatrx® NANO 10 slides semi-automated work station for small size FISH laboratory requirement with provision of manual pipetting of FISH probes, DAPI and costly reagents. 10 different protocols in combination of histology and cytology specimens/ probes can be processed at a time Xmatrx® MINI 160 10 slides manual FISH processing platform with provision of on board pretreatment, dewaxing and washing. 10 different protocols in combination of histology and cytology specimens/probes can be processed at a time www.biogenex.com eFISHiency 1. eFISHiency probes We offer a wide range of probes for cytology specimens and histology tissues. Detection and Diagnosis of Genetic Aberrations Locus-Specific Probes • Bind to specific small sequences on chromosomes • Detect structural abnormalities (e.g. gene amplifications, deletions and duplications) Alphoid or Centromeric Repeat Probes • Detect repetitive sequences found in middle of each chromosome • Serve as enumeration probe to determine gene copy and chromosome numbers Whole Chromosome Probes • Apply single or multi-color probes to label each chromosome in its own unique color • Include gene fusion and break-apart probes to assess translocations and rearrangements internationalcs@biogenex.com 161 eFISHiency Sr # eFISH Probes Cat. No. 1 eFISH MDM2/CEN 12 Dual Color Probe FP038-10XE/20XE 2 eFISH CEN 17/HER2 Dual Color Probe FP039-10XE/20XE 3 eFISH EGFR/CEN 7 Dual Color Probe FP040-10XE/20XE 4 eFISH p16/CEN 9 Dual Color Probe FP041-10XE/20XE 5 eFISH FGFR1/CEN 8 Dual Color Probe FP042-10XE/20XE 6 eFISH NMYC/2q11 Dual Color Probe FP043-10XE/20XE 7 eFISH 1p36/1q25 Dual Color Probe FP044-10XE/20XE 8 eFISH 19q13/19p13 Dual Color Probe FP045-10XE/20XE 9 eFISH VHL/CEN 3 Dual Color Probe FP046-10XE/20XE 10 eFISH MET/CEN 7 Dual Color Probe FP047-10XE/20XE 11 eFISH EWSR1 Dual Color Break Apart Probe FP048-10XE/20XE 12 eFISH SYT Dual Color Break Apart Probe FP049-10XE/20XE 13 eFISH CHOP Dual Color Break Apart Probe FP050-10XE/20XE 14 eFISH TFE3 Dual Color Break Apart Probe FP051-10XE/20XE 15 eFISH COL1A1/PDGFB Dual Color Dual Fusion Probe FP052-10XE/20XE 16 eFISH PDGFB Dual Color Break Apart Probe FP053-10XE/20XE 17 eFISH COL1A1 Dual Color Break Apart Probe FP054-10XE/20XE 18 eFISH FGFR2/CEN 10 Dual Color Probe FP055-10XE/20XE 19 eFISH ALK Dual Color Break Apart Probe FP056-10XE/20XE 20 eFISH CCND1/IGH Dual Color Dual Fusion Probe FP057-10XE/20XE 21 eFISH FUS Dual Color Break Apart Probe FP058-10XE/20XE 22 eFISH PIK3CA/CEN 3 Dual Color Probe FP059-10XE/20XE 23 eFISH ROS1 Dual Color Break Apart Probe FP060-10XE/20XE 24 eFISH RET Dual Color Break Apart Probe FP061-10XE/20XE 25 eFISH TP53/CEN 17 Dual Color Probe FP062-10XE/20XE 26 eFISH CCND1/CEN 11 Dual Color Probe FP063-10XE/20XE 27 eFISH CMYC Dual Color Break Apart Probe FP064-10XE/20XE 28 eFISH CMYC/CEN 8 Dual Color Probe FP065-10XE/20XE 29 eFISH TERT/5q31 Dual Color Probe FP066-10XE/20XE 30 eFISH CMYC/IGH Dual Color Dual Fusion Probe FP067-10XE/20XE 31 eFISH EGR1/5p15 Dual Color Probe FP068-10XE/20XE 32 eFISH CCND1 Dual Color Break Apart Probe FP069-10XE/20XE 33 eFISH IGH Dual Color Break Apart Probe FP070-10XE/20XE 34 eFISH BCR/ABL Dual Color Dual Fusion Probe FP071-10XE/20XE 35 eFISH AML1/ETO Dual Color Dual Fusion Probe FP072-10XE/20XE 36 eFISH PML/RARA Dual Color Dual Fusion Probe FP073-10XE/20XE 37 eFISH BCL2/IGH Dual Color Dual Fusion Probe FP074-10XE/20XE 38 eFISH BIRC3/MALT1 Dual Color Dual Fusion Probe FP075-10XE/20XE 39 eFISH ETV6/RUNX1 Dual Color Dual Fusion Probe FP076-10XE/20XE 162 www.biogenex.com eFISHiency 2. eFISH pretreatment kits Sr # eFISH Kit Description Automation Platform 1 eFISH Histo eFISH Kit for Histology tissue (FFPE) Xmatrx, NANO and MINI 2 eFISH Cyto eFISH Kit for Cytology Specimen Xmatrx, NANO and MINI 3 eFISH U eFISH Kit Universal for both histology and cytology specimen Manual I. eFISH Histo - Cat # DF500-20XE eFISH Histo is designed to meet the FISH processing requirement of FFPE tissues. It contains buffers and reagents for pretreatment and post hybridization stringency washes. This kit is compatible on automation platforms, Xmatrx ELITE, Xmatrx NANO and Xmatrx MINI. Sr # Reagents Volume/amount Part No. 1 eFISH pretreatment Solution 20 ml HK983-20XE 2 eFISHProtease 1 Buffer 5X20 ml HK981-20XE 3 eFISH reagents A 20 ml HK972-20XE 4 Pepsin powder 1 5X10 mg HK602-10XE 5 eFISH Wash buffer 1 (20X) 200 ml HK604-20XE II. eFISH Cyto - Cat # DF510-20XE eFISH Cyto is designed to meet the FISH processing requirement of cytology and hematology specimens. It contains buffers and reagents for pretreatment and post hybridization stringency washes. This kit is compatible on automation platforms, Xmatrx ELITE, Xmatrx NANO and Xmatrx MINI Sr # Reagents Volume/amount Part No. 1 eFISH reagents A 20 ml HK972-20XE 2 eFISH reagents B 20 ml HK973-20XE 3 eFISH Protease 2 Buffer 5X20 ml HK982-20XE 4 eFISH Formaline Fixative 20 ml HK603-20XE 5 Pepsin powder 2 5X2 mg HK602-02XE 6 eFISH Wash buffer 1 (10X) 200 ml HK604-20XE 7 eFISH Wash buffer 2 (10X) 200 ml HK605-20XE III. eFISH U - Cat # DF-530-40KE eFISH U, is a bulk reagents universal kit, designed to meet the FISH processing requirement of both histology FFPE tissue and cytology/ hematology specimen. It contains buffers and reagents for pretreatment and post hybridization stringency washes. Sr # Reagents Volume/amount Part No. 1 eFISH pretreatment Solution 500 ml HK983-YRXE 2 eFISH reagents A 500 ml HK972-YRXE 3 eFISH reagents B 500 ml HK973-YRXE 4 eFISH Protease 1 Buffer 500 ml HK981-YRXE 5 eFISH Protease 2 Buffer 500 ml HK982-YRXE 6 eFISH Wash buffer 1 (10X) 50 ml HK604-YRXE 7 eFISH Wash buffer 2 (10X) 50 ml HK605-YRXE 8 eFISH Formaline Fixative 500 ml HK603-YRXE 9 Pepsin powder 1U 25 mg X 10 HK602-25KE 10 Pepsin powder 2 U 5 mg X 10 HK602-05KE internationalcs@biogenex.com 163 eFISHiency 3. eFISH Processing systems True eFISHiency Now FISH can be the nexus of a more efficient and more productive laboratory. With a family of Xmatrx® systems, you have the freedom to attend to more demanding tasks while delivering high-quality and standardized results every time. Microtome to Microscope COMPLETE AUTOMATION 33 Steps Reduced to 3 Steps 7.5 Hours Reduced to 30 Minutes eFISHiency System for FISH Automation FISH - AUTOMATION 33 Steps Reduced to 6 Steps 7.5 Hours Reduced to 1 Hour eFISHiency Workstation MANUAL 33 Steps 7.5 Hours 164 eFISHiency WORKSTATION 33 Steps 7.5 Hours Reduced to 5.5 Hours www.biogenex.com eFISHiency Rethink the way FISH fits into your workflow Microtome to Microscope • The world’s irst and only fully automated front-end FISH processing system • Run up to 40 slides under multiple protocols • Reduce hands-on tech time from 7.5 hours to 30 minutes 33 Steps Reduced to 3 eFISHiency System for FISH Automation • On-board dewaxing, oil seal and inal coverslip after DAPI • Add micro-reagents manually to save cost • Run 10 different protocols at the same time • Intelligent SMS information for alerts 33 Steps Reduced to 6 eFISHiency Workstation • eFISHiency Workstation for manual FISH assay • Hybridizer with eXACTTM temperatures • 10 Independently programmable thermal cyclers • Built-in touch screen display • Manual coverslip application and removal Accessories Oil stamp Coverslip stand internationalcs@biogenex.com Suction pen 165 eFISHiency Family of Xmatrx® Systems to Provide Optimum Workflow Solutions for Your Laboratory Needs With superior staining quality and enhanced laboratory productivity in mind, we have developed a family of Xmatrx® Systems to produce standardized results and provide optimum workflow solutions for your laboratory needs by: •Streamlining lab workflow •Increasing throughput •Improving reproducibility • Freeing up critical resources Placement of slides on eXACTTM temperature controlled blocks Automated application of oil for sealing reaction chamber (micro-chamber) Automated dispensing of micro-reagents (proteinase, probe and DAPI) Automated application and removal of coverslips Automated wash and airblow to dry slides Automated mounting and final coverslip after DAPI Placement of slides on eXACTTM temperature controlled blocks Automated application of oil for sealing reaction chamber (micro-chamber) Manual dispensing of micro-reagents (proteinase, probe and DAPI) Automated application and removal of coverslips Automated wash and airblow to dry slides Automated mounting and final coverslip after DAPI Placement of slides on eXACTTM temperature controlled blocks Manual application of oil with anoil stamp for sealing reaction chamber [micro-chamber] Manual dispensing of micro-reagents (proteinase, probe and DAPI) Manual application and removal of coverslips with a suction pen Manual wash and dry with aid of heat Manual mounting and coverslip after DAPI 166 www.biogenex.com Special Stains Special Stains Acid Fast Bacteria (AFB) Blue Stain Alcian Blue pH 2.5 Stain Cat. No. (Xmatrx): SS025-50X Cat. No. (Xmatrx): SS027-50X Specificity: Mycobacterium tuberculosis Cat. No. (i6000): SS027-YAK Specificity: Expected Results: Red (Acid fast bacilli) and Blue (non-acid fast bacteria) Acid mucins and mucopolysaccharides Expected Results: Acid mucopolysaccharides stain blue; nuclei stain pink to red; cytoplasm stains pale pink Lung tissue infected with Mycobacterium tuberculosis Alcian blue staining of colon tissue This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System. The Acid Fast Stain is for demonstration of mycobacteria, especially Mycobacterium tuberculosis, in tissue sections and smears. Mycobacteria are difficult to demonstrate by other staining techniques due to the fatty acid capsule surrounding the organism. This fatty acid covering influences the degree to which stains may penetrate and subsequently be removed. Acid Fast staining may be used for the demonstration of mycobacteria including M. tuberculosis, M. kansasii, M. avium, and M. leprae. Tuberculosis (TB) remains a major health threat, especially in developing countries. This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Alcian Blue offers comprehensive staining for acid mucins and stains very intensely over a varying pH range. Strongly sulphated mucins stain below pH 1.0, weakly sulfated mucins stain at pH 2.5 and above, while sialomucins (depending on their type) stain between pH 1.5 and 3.2. Alcian Blue is a water soluble, high molecular weight, copper phthalocyanin basic dye. This Alcian Blue kit stains mucins at pH 2.5. The staining results are insoluble in water or alcohol and do not fade appreciably over time. Acid Fast Bacteria (AFB) Green Stain Alizarin Red Stain Cat. No. (Xmatrx): SS059-50X Cat. No. (Manual): SS052-50K specificity: Mycobacterium spp Specificity: Calcium in tissue section Expected Results: Acid fast bacilli stains red while the background stains green Expected Results: Calcium deposits stain orange-red Lung tissue infected with Mycobacterium tuberculosis Calcium deposits in bone tissue Mycobacteria are difficult to demonstrate by other staining techniques due to the fatty acid capsule surrounding the organism. This fatty acid covering influences the degree to which stains may penetrate and subsequently be removed. Acid Fast staining may be used for the demonstration of mycobacteria including M. tuberculosis, M. kansasii, M. avium, and M. leprae. Tuberculosis (TB) remains a major health threat, especially in developing countries. A major cause of death in AIDS patients in Africa is TB. M. kansasii and M. avium are also frequently responsible for opportunistic infections in these AIDS patients. Acid Fast remains one of the most common stains used. This product is intended for in vitro diagnostic use and is used to identify calcium in tissue sections. Calcium forms an Alizarin Red S-calcium complex in a chelation process and the reaction is birefringent. The reaction is not strictly specific for calcium, since magnesium, manganese, barium, strontium, and iron may interfere, but these elements usually do not occur in sufficient concentration to interfere with the staining. Amyloid (Congo Red) Stain Alcian Blue/PAS Stain Alcian Blue staining of colon tissue SS028-50X SS028-YAK Cat. No. (Xmatrx): SS026-50X Cat. No. (i6000): Cat. No. (i6000): SS026-YAK Specificity: Specificity: Neutral and acidic mucosubstances Amyloid in tissue sections Expected Results: Expected Results: Acid mucins stain blue, neutral mucins stain magenta, mixtures of mucins stain blue/purple; nuclei stain deep blue Amyloid stains red to deep pink; nuclei stain blue; elastic fibers stain light pink This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Alcian Blue PAS is a combined method utilizing the properties of both the PAS and Alcian Blue methods to demonstrate the full complement of tissue proteoglycans. Alcian Blue offers comprehensive staining for acid mucins at pH 2.5. The staining results are insoluble in water or alcohol and do not fade appreciably over time. Neutral mucins which are solely PAS positive will subsequently be demonstrated in a contrasting manner. Where mixtures occur, the resultant colour will depend upon the dominant moiety. 168 Cat. No. (Xmatrx): Amyloid staining of liver tissue This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Amyloids are insoluble fibrous protein aggregates that erroneously interact with one another or other cell components forming insoluble fibrils. Abnormal accumulation of amyloid fibrils in organs may lead to amyloidosis and play a role in various neurodegenerative disorders. Amyloid deposits are extracellular and may become sufficiently large to cause damage to surrounding tissues. Congo Red is the gold standard method for diagnosis of amyloidosis. When stained with Congo Red and viewed through polarizing lenses amyloid will birefringe an apple green color under the microscope. www.biogenex.com Special Stains Azure A Stain Elastic Stain Cat. No. (Xmatrx): SS038-50X Cat. No. (Xmatrx): SS029-50X Cat. No. (i6000): SS038-YAK Specificity: Specificity: Mast cells in skin tissue (neurofibroma) Staining of elastin in tissue sections Expected Results: Elastic fibers (shades of dark blue); Collagen fibers (shades of red); Nuclei (shades of blue to black); Other tissue elements (shades of yellow) Expected Results: Azure A staining of mast cells in skin tissue Mast cell granules stain purple; Nuclei stain blue; Background stains pale blue This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Mast cells are normally present in small numbers in the connective tissue of all organs, but particularly in the dermal layer of skin and are identified by their cytoplasmic granules. Increased numbers of mast cells are found in many pathological conditions. Mast cell hyperplasia in the skin (mastocytosis) manifests with skin lesions and may present with symptoms of urticaria and flushing due to the chemical mediators released during mast cell degranulation. Elastic fibers in aorta tissue This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System. The elastic staining procedure can demonstrate the characteristics of emphysema (i.e., atrophy of elastic tissue), arteriosclerosis (i.e., thinning and loss of elastic fibers) and various other vascular diseases. Giemsa Stain Bielschowsky’s Silver Stain Cat. No. (Xmatrx): SS055-50X Cat. No. (i6000): SS055-YAK Specificity: Nerve fibers Expected Results: Axons, neurofibrillary tangles and senile plaques (black); Background (yellow to brown) Bielschowsky’s staining of brain tissue Bielschowsky’s stain is a silver stain technique used to demonstrate neurofibrillary tangles, nerve fibers and senile plaques in Alzheimer’s disease. Colloidal Iron Stain Cat. No. (Xmatrx): SS054-50X Cat. No. (i6000): SS054-YAK Specificity: Carboxylated and sulfated mucopolysaccharides and glycoproteins Expected Results: Collodal Iron staining of adeno carcinoma of intestine Acid mucopolysaccharide and sialomucins appear deep blue, nuclei appear pink-red and cytoplasm appears pink This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated System and also for i6000 staining. The Colloidal Iron Stain Kit is used for the demonstration of carboxylated and sulfated mucopoly-saccharides and glycoproteins and is suitable for any well-fixed paraffin embedded tissue cut at 5 microns. Colloidal ferric ions are, at low pH, absorbed principally by carboxylated and sulfated mucosubstances. internationalcs@biogenex.com Cat. No. (Xmatrx): SS049-50X Cat. No. (i6000): SS049-YAK Specificity: Helicobacter pylori Expected Results: Helicobacter pylori and nuclei stain dark blue, and cytoplasm stains pink Giemsa stain for H.pylori in tissue sections This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. It is recognized that Helicobacter pylori is the cause of most stomach and duodenal ulcers. Approximately 95% of persons with gastric ulcers and 100% of persons with chronic gastritis have this bacterium within the stomach. The organism has not been found in healthy persons. Those without stomach ulcers or gastritis. H. pylori are known to bind to the O blood-group structure (a particular series of sugars) present in gastric epithelial cells. Gomori’s Trichrome Blue Stain Cat. No. (Xmatrx): SS033-50X Cat. No. (i6000): SS033-YAK Specificity: Muscle and collagen fibers Expected Results: Muscle fibers stain red; Collagen stains blue; Nuclei stain blue-black Gomoris Trichrome staining of Fallopian tube This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Trichrome stains have historically been used to distinguish collagen from muscle tissue. The term “trichrome” refers to a mix of three stains. These dyes stain nucleus, collagen and cytoplasm structures and are often in mordents such as phosphotungstic or phosphomolybdic acid. Use of trichrome stain may be useful in the study of diseases of connective tissue and muscle characterized by fibrotic and dystrophic changes and to differentiate between collagen and smooth muscle in tumors. 169 Special Stains Gomori’s Trichrome Green Stain Iron Stain Cat. No. (Xmatrx): SS034-50X Cat. No. (Xmatrx): SS030-50X Cat. No. (i6000): SS034-YAK Cat. No. (i6000): SS030-YAK Specificity: Muscle and collagen fibers Specificity: Expected Results: Nuclei (blue), Collagen (green), Muscle Fiber (red) Detection of ferric iron in tissue sections, and blood or bone marrow films Expected Results: Iron (bright blue); Nuclei (red); Cytoplasm (pink) Gomori’s Trichrome staining of Fallopian tube Spleen tissue with iron deposits This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Trichrome stains have historically been used to distinguish collagen from muscle tissue. The term “trichrome” refers to a mix of three stains. These dyes stain nucleus, collagen and cytoplasm structures and are often in mordants such as phosphotungstic or phosphomolybdic acid. Use of trichrome stain may be useful in the study of diseases of connective tissue and muscle characterized by fibrotic and dystrophic changes and to differentiate between collagen and smooth muscle in tumors. This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Small amounts of ferric iron may be found in bone marrow and spleen. Very large deposits may be seen in conditions such as hemachromatosis and hemosiderosis. Jones’ Basement Membrane Stain Gram Stain Cat. No. (Xmatrx): SS037-50X Cat. No. (i6000): SS037-YAK Specificity: Gram positive and Gram negative microorganisms Expected Results: Gram positive(blue), Gramnegative (pink to red) Gram staining of gram-negative bacilli This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. The Gram Stain procedure continues to be one of the initial screening tests for detecting bacteria in wounds, sputum, pus, tissues, etc. Although the exact chemical nature of the method is yet to be established, it is known that Gram positive organisms, staining a deep blue color, have a cell wall containing teichoic acid, while Gram negative organisms, staining a red-pink color, have cell walls containing lipopolysaccharides. SS042-50X Cat. No. (i6000): SS042-YAK Specificity: Fungi in tissue sections Expected Results: Fungi stain gray to black with a light green background GMS staining for fungi in tissue sections This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Human bodies are regularly exposed to fungi spores from many species. Patients with a diminished or compromised immune system show greater susceptibility and incidence of fungal infections. Some fungi may elicit a range of host reactions from oxidative, necrotising to granulomatous. Other fungi produce little cellular response to indicate their presence. Most fungi are relatively large and their cell walls are rich in polysaccharide. These polysaccharides can be oxidized to dialdehydes and detected with Schiff’s reagent or silver solution. 170 SS058-50X Cat. No. (i6000): SS058-YAK Specificity: Basement membrane Expected Results: Basement membrane (black); Nuclei (red); Background (pink) Jones’ basement membrane staining of kidney with glomeri This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated System and also for i6000 staining. Hematoxylin and Eosin stained sections of most tissue do not distinguish between basement membranes well. In disorders such as membranous nephropathy or diabetes the basement membranes in the glomerulus become more conspicuous. Jones’ Basement Membrane stain kit is used to identify basement membranes, specifically glomerular and tubular membranes in renal tissue. Masson’s Trichrome Stain Grocott’s Methenamine Silver (GMS) for Fungi Stain Cat. No. (Xmatrx): Cat. No. (Xmatrx): Cat. No. (Xmatrx): SS035-50X Cat. No. (i6000): SS035-YAK Specificity: Muscle and collagen fibers Expected Results: Collagen (blue); Nuclei (black); Muscle, cytoplasm, keratin (red) Masson’s Trichrome staining of stomach tissue This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Trichrome stains have historically been used to distinguish collagen from muscle tissue. The term “trichrome” refers to a mix of three stains. These dyes often stain nucleus, collagen and cytoplasm structures in mordants such as phosphotungstic or phosphomolybdic acid. Use of Masson’s trichrome stain may be useful in the study of diseases of connective tissue and muscle characterized by fibrotic and dystrophic changes and to differentiate between collagen and smooth muscle in tumors. www.biogenex.com Special Stains Periodic Acid-Schiff (PAS) for Fungi Stain Mucicarmine Stain Cat. No. (Xmatrx): SS036-50X Cat. No. (Xmatrx): SS053-50X Cat. No. (i6000): SS036-YAK Cat. No. (i6000): SS053-YAK Specificity: Mucopolysaccharides Specificity: Expected Results: Mucins (deep rose); Nuclei (black); Other tissue elements (yellow) PAS for Fungi is specific for fungal organisms in tissue sections Expected Results: Fungi stain rose and background stains green to blue PAS staining of fungi in lung tissue Mucicarmine staining of small intestine This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Mucicarmine preferentially stains mucin with strong staining of epithelial mucins and poor staining of mucins of fibroblastic origin. Staining results may be used to identify primary tumor sites, distinguishing mucin-negative undifferentiated squamous cell lesions from mucinspositive adenocarcinomas and staining capsule of Cryptococcus. This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. PAS for fungi is a staining method used to identify fungi in formalin-fixed and paraffin-embedded tissues or autopsy tissues. Polysaccharides present in fungal cell walls are oxidized by the periodic acid to aldehydes. The aldehydes react with the Schiff reagent to yield rosecolored staining of fungi. Periodic Acid-Schiff (PAS) Stain (without Diastase)* Oil Red O Stain Cat. No. (Xmatrx): SS043-50X Cat. No. (i6000): SS043-YAK Specificity: Fat staining on frozen sections Expected Results: Fat (red); Nuclei (blue) Cat. No. (Xmatrx): SS032-50X Cat. No. (i6000): SS032-YAK Specificity: Glycogen glycoproteins; Mucopolysaccharides; Basement membrane and mucin Expected Results: Glycogen (red-purple); Background (blue) PAS staining of liver tissue Oil Red O staining of fat containing tissue This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Oil Red O stain is an oil soluble dye used to evaluate normal or abnormal fatty tissue. Abnormal deposits of fatty emboli may develop after a bone fracture or an injury that crushes fatty tissue. Periodic Acid-Schiff (PAS) Diastase Stain Cat. No. (Xmatrx): SS039-50X Cat. No. (i6000): SS039-YAK Specificity: PAS stains specifically glycogen, glycoproteins, mucopolysaccharides, basement membrane and mucin. PAS Diastase staining of liver tissue Expected Results: Nuclei stain blue; glycogen and other carbohydrates stain red to pink; No stained glycogen seen in diastasedigested tissue This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. PAS is a stain that typically gives a magenta color in the presence of glycogen. Diastase is an enzyme that breaks down glycogen into smaller sugar units, maltose and glucose that are washed out of the section. Differences in the intensities of the two stains (PAS and PAS-D) can be attributed to different glycogen concentrations and can be used to semi-quantify glycogen in samples. * This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. The PAS reaction in tissue sections is useful for outlining tissue structures such as basement membranes, capsules and blood vessels. This staining procedure may also be used for the demonstration of fungal organisms in tissue sections. PAS staining is mainly used for staining structures containing a high proportion of carbohydrate macromolecules (glycogen, glycoprotein, proteoglycans) typically found in connective tissues, mucus, the glycocalyx, and basal laminae. Renal Masson’s Trichrome Stain Renal Masson’s Trichrome staining of kidney Cat. No. (Xmatrx): SS050-50X Cat. No. (i6000): SS050-YAK Specificity: Collagen Expected Results: Fibrinoid and immune deposits appear red; Basement membrane & collagen stain green and nuclei stain blue Trichrome stains have historically been used to distinguish collagen from muscle tissue. The term “trichrome” refers to a mix of three stains. These dyes often stain nucleus, collagen and cytoplasm structures in mordants such as phosphotungstic or phosphomolybdic acid. To be released soon internationalcs@biogenex.com 171 Special Stains Sudan Black B Stain* Reticulin/No Counterstain Cat. No. (Xmatrx): SS046-50X Cat. No. (Xmatrx): SS041-50X Cat. No. (i6000): SS046-YAK Cat. No. (i6000): SS041-YAK Specificity: Reticular fibers Specificity: Expected Results: Reticulin stains black without any counter stain Sudan Black B staining procedure is used to demonstrate neutral lipids and phospholipids in frozen tissue sections Expected Results: Fat stains blueblack and nuclei stain red Fat in frozen sections stained by Sudan black B Reticulin staining of liver tissue This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Ammonical silver stains are common methods for the demonstration of reticular fibers. These procedures may be used for the differential diagnosis of tumors such as carcinomas, sarcomas and lymphosarcomas. Reticulin/Nuclear Fast Red Stain Cat. No. (Xmatrx): SS047-50X Cat. No. (i6000): SS047-YAK Specificity: Reticular fibers Expected Results: Reticulin stains black with a pink to rose background This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Sudan Black B is a classical dye used for studying lipids in tissue sections. Sudan black B stain is used to differentiate fat cell tumors (liposarcomas) from other types of tumors. Abnormal deposits of fatty emboli may develop after a bone fracture or an injury that crushes fatty tissue. The fat stain may verify that an emboli caused death. When cell membranes or myelin degenerate, fatty substances may be formed and can be detected with this stain. Toluidine Blue Stain Cat. No. (Xmatrx): SS057-50X Cat. No. (i6000): SS057-YAK Specificity: Mast cell Expected Results: Mast cell granules and carboxylated mucins (purple); Nuclei (blue); Background (pale blue) Reticulin staining of kidney tissue Ammonical silver stains are common methods for demonstration of reticular fibers. These procedures may be used for the differential diagnosis of tumors such as carcinomas, sarcomas and lymphosarcomas. Safranin O Stain Safranin O staining of cartilage Cat. No. (Xmatrx): SS040-50X Cat. No. (i6000): SS040-YAK Specificity: Cartilage, mucin, and mast cell granules Expected Results: Nuclei stain black, cytoplasm stains gray-green green, cartilage, mucin & mast cell granules stain orange to red This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Safranin O staining is used for the detection of cartilage, mucin, and mast cell granules on formalin-fixed paraffin-embedded and frozen tissue sections. In this staining, cartilage and mucin stain orange to red, nuclei stain black and the background is stained gray green. Safranin O is used to demonstrate any changes that occur in articular diseases. Toluidine blue staining of mast cells in skin tissue This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System. Mast cells normally present in the connective tissue of all organs are increased in many pathological conditions. Mast cell granules which are refractile and not readily identified in H&E stained sections are well demonstrated by the Toluidine blue staining method. Van Gieson Stain Van Gieson staining of stomach tissue Cat. No. (Xmatrx): SS044-50X Cat. No. (i6000): SS044-YAK Specificity: Collagen and smooth muscle in tumors Expected Results: Nuclei (blue/ black); Collagen (red); Cytoplasm, muscle fibrin and red blood cells (yellow) This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Van Gieson Stain is used to differentiate between collagen and smooth muscle in tumors and to demonstrate the increase of collagen in diseases. This method combines two or more anionic dyes and relies on differential binding by tissue components. The differentiation is determined by a combination of differences in the relative size of the dye molecules, differences in the physical structure of the tissue, and differences in the amino acid composition of tissue elements. * To be released soon 172 www.biogenex.com Special Stains Von Kossa Stain Cat. No. (Manual): SS045-50K Specificity: Calcium salts in tissue sections Expected Results: Calcium salts (black or brown black); Nuclei (red) & cytoplasm (pink) Von Kossa staining of calcified node This product is intended for in vitro diagnostic use to demonstrate deposits of calcium in tissues. Abnormal deposits of calcium may be found in any area of the body. With the H&E stain, calcium appear deep blue-purple. Von Kossa staining is specific for demonstrating deposits of calcium or calcium salt and is not specific for the calcium ion itself. When tissue sections are treated with a silver nitrate solution, the silver is deposited by replacing the calcium, reduced by the strong light, and thereby visualized as metallic silver. Additional methods are sometimes employed along with this technique to confirm the presence of calcium such as Alizarin Red, which detects calcium deposits. Special Stains (Xmatrx®) Product Name Pack size Cat. No. Acid-Fast Bacteria (AFB) Blue Stain 50 Tests SS025-50X Acid-Fast Bacteria (AFB) Green Stain 50 Tests SS059-50X Alcian Blue/PAS Stain 50 Tests SS026-50X Alcian Blue pH 2.5 Stain 50 Tests SS027-50X Amyloid (Congo Red) Stain 50 Tests SS028-50X 50 Tests SS038-50X Bielschowsky’s Silver Stain 50 Tests SS055-50X Colloidal Iron Stain 50 Tests SS054-50X Elastic Stain 50 Tests SS029-50X Giemsa Stain 50 Tests SS049-50X Gomori's Trichrome Blue Stain 50 Tests SS033-50X Gomori's Trichrome Green Stain 50 Tests SS034-50X Gram Stain 50 Tests SS037-50X Grocott's Methenamine Silver (GMS) Stain 50 Tests SS042-50X Iron Stain 50 Tests SS030-50X Jones’ Basement Membrane Stain* 50 Tests SS058-50X Masson's Trichrome Stain 50 Tests SS035-50X Mucicarmine Stain 50 Tests SS036-50X Oil Red O Stain* 50 Tests SS043-50X Periodic Acid-Schiff (PAS) Diastase Stain 50 Tests SS039-50X Periodic Acid-Schiff (PAS) for Fungi Stain 50 Tests SS053-50X Periodic Acid-Schiff (PAS) Stain (without Diastase)* 50 Tests SS032-50X Renal Masson’s Trichome Stain 50 Tests SS050-50X Reticulin/No Counterstain 50 Tests SS046-50X Reticulin/Nuclear Fast Red Stain 50 Tests SS047-50X Safranin O Stain 50 Tests SS040-50X Azure A Stain * * * Sudan Black B Stain 50 Tests SS041-50X Toluidine Blue Stain 50 Tests SS057-50X Van Gieson Stain 50 Tests SS044-50X To be released soon internationalcs@biogenex.com 173 Special Stains Special Stains (i6000TM) Product Name Pack size Cat. No. Alcian Blue/PAS Stain 50 Tests SS026-YAK Alcian Blue pH 2.5 Stain 50 Tests SS027-YAK Amyloid (Congo Red) Stain 50 Tests SS028-YAK Azure A Stain 50 Tests SS038-YAK Bielschowsky’s Silver Stain* 50 Tests SS055-YAK Colloidal Iron Stain 50 Tests SS054-YAK Giemsa Stain 50 Tests SS049-YAK Gomori's Trichrome Blue Stain 50 Tests SS033-YAK Gomori's Trichrome Green Stain 50 Tests SS034-YAK Gram Stain 50 Tests SS037-YAK Grocott's Methenamine Silver (GMS) for Fungi Stain 50 Tests SS042-YAK Iron Stain 50 Tests SS030-YAK Jones’ Basement Membrane Stain 50 Tests SS058-YAK Masson's Trichrome Stain 50 Tests SS035-YAK Mucicarmine Stain 50 Tests SS036-YAK Oil Red O Stain* 50 Tests SS043-YAK Periodic Acid-Schiff (PAS) Diastase Stain 50 Tests SS039-YAK Periodic Acid-Schiff (PAS) for Fungi Stain 50 Tests SS053-YAK Periodic Acid-Schiff (PAS) Stain (without Diastase)* 50 Tests SS032 -YAK Renal Masson's Trichrome Stain 50 Tests SS050-YAK Reticulin/No Counterstain 50 Tests SS046-YAK Reticulin/Nuclear Fast Red Stain 50 Tests SS047-YAK Safranin O Stain 50 Tests SS040-YAK * * Sudan Black B Stain 50 Tests SS041-YAK Toluidine Blue Stain 50 Tests SS057-YAK Van Gieson Stain 50 Tests SS044-YAK Product Name Pack size Cat. No. Alizarin Red Stain 50 Tests SS052-50K Von Kossa Stain 50 Tests SS045-50K Special Stains (Manual) * To be released soon 174 www.biogenex.com Ancillary Reagents Ancillary Reagents internationalcs@biogenex.com 175 Ancillary Reagents Buffers and Diluents Buffers and diluents are available for most applications in Immunohistochemistry and in situ Hybridization. General buffers, such as PBS and TBS can be used for washing/rinsing of slides. BioGenex offers several diluents for specific antibody or reagent products. The Common Antibody Diluent and Enhanced Antibody Diluent have been developed for use with all antibodies in immunohistochemistry and have been specifically optimized for use with BioGenex antibodies and reagent products. These diluents enable enhancement of signal-to-noise ratio of staining when used optimally and also help in maintaining the antibody specificity and stability. The Enhanced Antibody Diluent, in addition to all the above features, contains chemical compounds to enhance antigen-antibody interaction and affinity. In order to achieve desired staining pattern and intensity, the titers of antibodies or concentrations of reagents may need to be optimized. Buffers - General Product Name Pack size Cat. No. Phosphate Buffered Saline, 25X Concentrate (pH 7.6 upon dilution; makes 3L total) 120 ml HK091-5KE Tris Buffer, dried powder (wash buffer) (pH 7.6, 0.1M upon reconstitution; makes 3L total) 3 packets HK098-5K Pack size Cat. No. 500 ml HK583-5KE Product Name Pack size Cat. No. Link Diluent (not for use with concentrated Goat Link, Cat. No. HK327-UG) 100 ml HK165-5KE Streptavidin Peroxidase Diluent (use with concentrated peroxidase labels) 100 ml HK157-5KE Common Antibody Diluent (Contains Sodium Azide) 100 ml HK156-5KE Enhanced Antibody Diluent (RTU) 100 ml HK941-YAKE Buffers - Wash Product Name TM Super Sensitive Wash Buffer (20X concentrated) Diluent 176 www.biogenex.com Ancillary Reagents Blocking Reagents Avidin/Biotin Blocking Kit: Certain tissues especially liver, kidney, and GI tract are rich in biotin. Use of the Avidin/Biotin Blocking kits ensures the blocking of all endogenous biotin, biotin receptors, or avidin binding sites present in the tissue. Pre-treatment of tissues with avidin blocking should always be followed with biotin blocking. Peroxide Block: Endogenous peroxidase is most commonly encountered in red blood cells, kidney, and liver tissue. Peroxide Block should be used prior to application of primary antibody when Horseradish Peroxidase (HRP) is used as the labeling enzyme, and if it is necessary to block endogenous peroxidase activity in the tissue being stained. Power BlockTM: This is a blocking reagent for reducing nonspecific background in immunoassays. A truly universal block, it is suitable for use in immunohistochemistry, immunocytochemistry, ELISA methods, and immunogold techniques. The Power BlockTM reagent contains buffer, casein and preservative and also works well as an antibody diluent and washing medium. Protein Block: This can be used to reduce background staining due to non-specific binding of the primary or secondary antibodies to the tissue. Protein Block should be applied immediately prior to the primary antibody. Levamisole: This is used to reduce background staining of alkaline phosphatase-based detection systems by inhibiting endogenous alkaline phosphatase in most tissues. It is intended for use with detection systems that employ intestinal alkaline phosphatase as the enzyme label. Levamisole is added to an alkaline phosphatase chromogen (Fast Red or BCIP/NBT). Product Name Pack size Cat. No. Avidin/Biotin Blocking Kit (RTU) 60 slides HK102-5K Peroxide Block 6 ml HK111-5K Peroxide Block 50 ml HK111-50K Power BlockTM Universal Blocking Reagent (10X concentrated) 100 ml HK085-5KE 25 ml BS-1310-25 Protein Block / Normal Goat Serum (for use with Mouse & Rabbit Link and Detection Systems), including MultiLink® 6 ml HK112-5K Protein Block / Normal Goat Serum (for use with Mouse & Rabbit Link and Detection Systems), including MultiLink® 50 ml HK112-9KE Protein Block/Normal Rabbit Serum (for use with Goat and Rat link and Detection System) 6 ml HK114-5K TM Power Block Universal Blocking Reagent (Ready-to-use) internationalcs@biogenex.com 177 Ancillary Reagents Substrates and Chromogens BioGenex offers complete Substrate Packs for immunohistochemical staining with alkaline phosphatase and peroxidase labels. The kits are designed to reduce substrate preparation time and minimize exposure to chemical hazards. In the subsequent section that covers counterstains and mounting media, a chart has been provided that summarizes the substrates offered, indicating enzyme and standard mounting media compatibility. BioGenex also offers "One-Step" AEC Solutions for staining convenience. The solution is ready-to-use and eliminates the need for any reagent mixing. This product provides crisp and clear staining patterns and allows excellent visualization of cellular components. Color development is rapid, i.e., 5 minutes or less in most cases. Features • • • • High Resolution AEC and Liquid DAB Rapid Development Time Ready-to-use Solutions Long-Term Stability Substrate Packs for immunohistochemical staining with peroxidase Product Name Pack size Cat. No. AEC One Step Solution (Recommended for Manual Use) 6 ml HK139-06K AEC One Step Solution (Recommended for use in Automation) 50 ml HK139-50KE AEC Substrate Pack (AEC chromogen 3 ml, H2O2 Substrate/Buffer 11 x 2.5 ml) 250 slides HK092-5K AEC Substrate Pack, Concentrated (AEC chromogen 2 x 6 ml, Concentrated H2O2 Substrate Buffer 3 x 17.5 ml) 2500 slides HK129-5KE Super Sensitive™ DAB (RTU) (Liquid DAB 5 ml, Stable Substrate Buffer 100 ml) 1000 slides HK542-XAKc Liquid DAB chromogen 4 ml HK124-7K a AEC (For Aqueous Mounting) DABb (For Permanent Mounting) a b c AEC, 3-amino-9-ethylcarbazole DAB, 3,3'-diaminobenzidine tetrahydrochloride Ideal for use with product QD440-XAK (please refer to page 139) 178 www.biogenex.com Ancillary Reagents Substrates and Chromogens Substrate Packs for Immunohistochemical Staining with Alkaline Phosphatase Elegance Red (For Permanent Mounting) Product Name Pack size Cat. No. Elegance Red Substrate Pack (Naphthol Phosphate Substrate, Permanent Red activator, Permanent Red Chromogen, Tris Buffer) 500 slides HK144-5KE Product Name Pack size Cat. No. Fast Red Substrate Pack 11 Fast Red tablets, 11 x 5 ml Naphthol Phosphate Buffer 500 slides HK182-5KE Naphthol Phosphate Substrate (for use with Fast Red Tablets) 15 ml HK180-7K Product Name Pack size Cat. No. New Fuchsin Substrate Pack (2.5 ml. New Fuchsin Chromogen, 2.5 ml New Fuchsin Activator, 5 ml of New Fuchsin Substrate, 8 x 5 ml New Fuchsin Tris Buffer) 400 slides HK183-5KE Fast Red (For Aqueous Mounting) New Fuchsin (For Permanent Mounting) Negative Control Sera/Immunoglobulins Each staining run should include a negative control slide to confirm reagent specificity. BioGenex, for this purpose, offers negative control sera/immunoglobulins which have been optimized for use as negative controls for our Super SensitiveTM, Ready-to-use antibodies. Negative controls are available for Mouse, Rabbit, Goat and Rat antibodies. Product Name Pack size Cat. No. Mouse, Super SensitiveTM Negative Control - gammaglobulin 17 ml HK119-7M Goat, Super SensitiveTM Negative Control 3 ml HK406-5G 3 ml HK407-5T Rabbit, Super SensitiveTM Negative Control 3 ml HK408-5R Rabbit, Super SensitiveTM Negative Control 17 ml HK408-7R TM Rat, Super Sensitive Negative Control internationalcs@biogenex.com 179 Ancillary Reagents Counterstains and Mounting Media BioGenex offers a selection of counterstains and mounting media for use in Immunohistochemistry and in situ Hybridization. In IHC and ISH procedures, a histological or cytological specimen affixed to a microscope slide is stained with a chromogen, counterstained, and mounted. The mounting medium may be used to attach a coverslip or may itself serve as a coverslip substitute. The choice of mounting medium depends on whether long-term ("permanent" mounting) or short-term preservation ("aqueous" mounting) is desired, and whether the mounting procedure is chemically compatible with the chromogen and the counterstain. BioGenex offers a unique SuperMount® Permanent Aqueous Mounting Medium. This is a polymer based mounting media that does not require the use of a coverslip. This innovative, patented mounting medium (BioGenex's U.S. Patent No. 5,492,837) is designed to preserve biological specimens for long-term storage. SuperMount® medium is compatible with most aqueous and organic-soluble dyes and chromogens including AEC, DAB, Elegance Red, Fast Red, New Fuchsin, BCIP/NBT, Rhodamine, Fluorescein, Texas Red, Phycoerythrin, Phycocyanin, and Fat Stain (Oil Red O). The refractive index of SuperMount® yields greater transparency and clarity of specimens to be examined under the microscope. When applied in a thin coat and allowed to harden, SuperMount® medium forms a highly transparent coating that permanently preserves stained tissue sections. SuperMount® can be used for the mounting of all biological specimens, including stained tissue sections, Cytospin preparations, and blood smears. BioGenex also offers Aqueous Mounting Medium, which on the other hand, is glycerol-based and does require the use of a coverslip. The compatibility chart on the next page summarizes the recommended mounting medium for different combinations of chromogen and counterstain. Chromogens Stain Color Solubility AEC Brick Red Alcohol/Xylene Soluble BCIP/NBT Blue-Purple Alcohol/Xylene Insoluble DAB Brown Alcohol/Xylene Insoluble Elegance Red Red Alcohol/Xylene Insoluble Fast Red Red Alcohol/Xylene Soluble New Fuchsin Red Alcohol/Xylene Soluble Counterstains Stain Color Solubility Pack size Cat. No. Hematoxylin, Mayer's (Non-alcohol-based) Blue Alcohol/Xylene Insoluble 6 ml HK100-5K* Hematoxylin, Mayer's (Non-alcohol-based) Blue Alcohol/Xylene Insoluble 250 ml HK100-9K* Mounting Media Solubility Pack size Cat. No. Aqueous Mounting Medium Uses aqueous solution 15 ml HK099-5K SuperMount® Permanent Mounting Medium Uses aqueous solution 15 ml HK079-5K SuperMount® Permanent Mounting Medium Uses aqueous solution 50 ml HK079-7K * For in vitro Diagnostic Use. 180 www.biogenex.com Ancillary Reagents Compatible Mounting Media for Chromogens and Counterstains a Counterstains AEC BCIP/NBT DAB Elegance Red Fast Red New Fuchsin Hematoxylin Aqueous/ SuperMount® Aqueous /Not recommended Aqueous/ Permanent/ SuperMount® Aqueous/ Permanent/ SuperMount® SuperMount® / Aqueous SuperMount® Methyl Green Not recommendeda Permanent Permanent Permanent Permanent Permanent Nuclear Fast Red Not recommendeda Aqueous/ Permanent/ SuperMount® Aqueous/ Permanent/ SuperMount® Not recommendeda Not recommendeda Not recommendeda Not Recommended (may have poor color based contrast) * For in vitro Diagnostic Use. Microscope Slides and Accessories OptiPlusTM Positive-Charged Microscope Slides provide a strong adhesive surface for tissues and cells to prevent tissue displacement during harsh pre-treatments such as enzymatic digestion and the microwave Antigen Retrieval method. These slides are ideal for automated systems. Additionally, each slide has a frosted end for easy labeling. The OptiPlusTM Positive-Charged Barrier Slides have all the advantages of our regular OptiPlusTM slides, but also contain hydrophobic barriers that allow the quantity of reagents per slide to be tailored to the size of the specimen. These slides eliminate reagent waste without the need to use a PAP pen, thereby reducing set-up time in manual assays as well as in automated systems. The slides come in three different configurations to accommodate different tissue sizes or multiple tissues per slide: 1. A single, full-size test area of 25 x 40 mm 2. A single 2/3-size test area of 25 x 30 mm, and 3. Three 1/3-size test areas per slide, each compartment measuring 25 x 15 mm Product Name Pack size Cat. No. PAP Pen 1 each XT001-PP OptiPlusTM Positive-Charged Microscope Slides 72 slides/box XT002-SL OptiPlusTM Positive-Charged Microscope Slides 20 boxes/case XT002-CL 72 slides/box XT134-SL OptiPlusTM Positive-Charged Barrier Slides (full test area) 20 boxes/case XT134-CL OptiPlusTM Positive-Charged Barrier Slides (2/3 test area) 72 slides/box XT013-SL OptiPlusTM Positive-Charged Barrier Slides (2/3 test area) 20 boxes/case XT013-CL OptiPlusTM Positive-Charged Barrier Slides (3 x 1/3 test areas) 72 slides/box XT014-SL 20 boxes/case XT014-CL TM OptiPlus TM OptiPlus Positive-Charged Barrier Slides (full test area) Positive-Charged Barrier Slides (3 x 1/3 test areas) For accessories to automated instruments please refer to Automation System section of the Catalog. internationalcs@biogenex.com 181 Ancillary Reagents 182 www.biogenex.com PR01 4 Kapp a 1234 56 Dr. Ja mes John Smith Black 12/2/ 2009 BioG enex PR Kap 014 1234 pa Dr. 56 Ja John mes 12/2 Black Smith /200 9 Bio Ge nex Tissue Control Tissue Control Positive Control Slides and Barrier Slides Positive control slides are made with tissue which has undergone processing identical to that of the test tissue. BioGenex provides positive control slides that enable one to confirm antibody reactivity. Barrier slides are positive control tissue slides with barriers to prevent loss of reagent. Pack size: Positive Control slides (5 slides per pack) Barrier slides (5 slides per pack) Antibody Clone Recommended Positive Control Control Slides Cat. No Barrier Slides Cat. No Aberrant Endothelial Cell 4A11 Tonsil / Lymph Node NA NA ACTH AH26 Pituitary FG-487N NA Actin, Muscle-Speciic HHF35 Heart / Skeleton Muscle FG-090M FB-090M Actin, Smooth Muscle 1A4 Colon FG-128M FB-128M Adenovirus A62020069P Adenovirus Infected Cells/Tissues FG-059M NA Alpha-1-Antichymotrypsin α1A88 Liver Carcinoma NA NA Alpha-1-Antitrypsin Polyclonal Hepatocellular Carcinoma FG-015P NA Alpha-Actinin JLN20 Muscle FG-097M NA Alpha-Fetoprotein (AFP) C3 Hepatocellular Carcinoma FG-008M FB-008M Alpha-Tubulin DM-1A Skin/Lung FG-121M NA Androgen Receptor F39.4.1 Prostate/Prostate Carcinoma FG-256M FB-256M Apolipoprotein E EP1373Y Liver FG-567N NA B Cell MB2 Tonsil FG-158M NA B Lyphocyte Antigen 36 (BLA.36) A27-42 B Lyphocytes/Hodgkin FG-231M FB-231M Bax Protein Polyclonal Breast Carcinoma NA NA Bcl-2 E17 Follicular Lymphoma and Reactive Lymph node Tissues FG-541N NA Bcl-2 EP36 Tonsil FG-723N FB-723N Bcl-2 Oncoprotein Bcl-2/100 Tonsil FG-287M FB-287M Bcl-6 LN22 Tonsil FG-708M FB-708M Bcl-x E18 Breast Carcinoma FG-462N NA Beta-Catenin E247 Colon Carcinoma FG-510N NA Beta-Tubulin DM-1B Skin/Lung FG-122M NA Beta-Tubulin II JDR3B8 Small Intestine/ Colon FG-176M NA Beta-Tubulin III SDL3D10 Heart FG-177M NA Beta-Tubulin IV ONS1A6 Lung FG-178M NA bFGF (Basic Fibroblast Growth Factor) bFGF88 Adenocarcinoma NA NA Blood Group Antigen Lewis A 7LE Stomach FG-303M NA Blood Group Antigen Lewis B 2-25LE Stomach FG-304M NA BOB-1 SP92 Tonsil FG731-N FB-731N BRCA1 Protein Polyclonal Hepatocellular Carcinoma NA NA Breast Cancer Antigen BCA 225 CU18 Breast Carcinoma FG-135M NA CA19-9 C241:5:1:4 Colon FG-424M FB-424M CA 125 (Ovarian Tumor Marker) Ov185:1 Ovary Carcinoma FG-429M FB-429M Caldesmon E89 Prostate FG-507N NA Caldesmon,High MW,Smooth muscle h-CD Breast Carcinoma NA NA Calponin CALP Breast Carcinoma FG-333M NA Calponin-1 (C-term) EP798Y Pleomorphic Adenoma FG-502N NA Calretinin 2E7 Brain Tissue/Appendix FG-583M NA Calretinin Polyclonal Brain FG-413P NA 184 www.biogenex.com Tissue Control Antibody Clone Recommended Positive Control Control Slides Cat. No Barrier Slides Cat. No Carcinoembryonic Antigen (CEA) B01-94-11M-P Normal Colon/ Colon Carcinoma FG-009M NA Carcinoembryonic Antigen (CEA) CEA88 Bowel Carcinoma FG-365M FB-365M Carcinoembryonic Antigen (CEA) Polyclonal Bowel/Colon Carcinoma FG-009P NA Catenin (p120) YE372 Breast Carcinoma FG-523N NA Catenin delta 1(p120) Polyclonal Breast Carcinoma FG-706P FB-706P Cathepsin D C15 Breast Carcinoma NA NA CD1a O10 Lymph Node FG-490M NA CD2 AB75 Lymph Node/Tonsil FG-438M NA CD3 Epsilon EP449E Spleen FG-477N NA CD3 (T Cell) UCHT1 Tonsil NA NA CD3 (T Cell) PS1 Tonsil FG-322M FB-322M CD3 and CD20 cocktail EP449E and L-26 Spleen/Tonsil FG-559C NA CD4 4B12 Tonsil FG-421M FB-421M CD4 EP204 Tonsil/T-cell Lymphoma FG-722N FB-722N CD4 + C8 Cocktail 4B12+EP1150Y Tonsil NA NA CD5 4C7 Tonsil FG-430M NA CD5 EP2952 Lymph Node FG-512N NA CD7 LP15 Tonsil FG-702M FB-702M CD8 1A5 Tonsil FG-422M FB-422M CD8 SP16 Tonsil NA NA CD8 (C-Term) EP1150Y Lymph Node FG-513N NA NA CD8 (T Cell,Suppressor/Cytotoxic) T8 Tonsil NA CD10 56C6 Lymphoma FG-451M NA CD10 and Cyclin D1 cocktail 56C6 and EPR2241 Breast Carcinoma/Lymphoma FG-561C NA CD11b (C3bi Receptor) M01 Tonsil NA NA CD11b/ITAM EPR1344 Spleen FG-546N NA CD11C EP1347Y Tonsil Tissue FG-555N NA CD14 EPR3653 Placenta FG-515N NA CD15 (Blood group antigen Lewis X) BRA4F1 Bone Marrow/Hodgkin FG-302M FB-302M CD16 2H7 Lymph Node/Breast FG-437M NA CD19 EP169 Tonsil FG-729N FB-729N CD20 CD20/C23 Spleen/Tonsil FG-537M NA CD20 (B Cell) L-26 Tonsil FG-238M FB-238M CD20 (B Cell) B1 Tonsil NA NA CD21 EP3093 Tonsil FG-497N NA CD21 (B Cell) B2 Tonsil/Lymph Node NA NA CD22 FPC1 Tonsil FG-439M NA CD23 Polyclonal Tonsil FG-460P NA CD25 4C9 Tonsil FG-453M NA CD29 JB1a Breast FG-298M NA CD30 (Ki-1 Antigen) Ber-H2 Hodgkin’s Lymphoma FG-327M NA CD30 (Ki-1 Antigen) HRS-4 Hodgkin’s Lymphoma NA NA CD31 (Endothelial Cell) JC/70A Colon Carcinoma FG-232M NA CD31 (PECAM-1) 9G11 Lymph Node FG-241M NA CD34 (Endothelial Cell) QBend/10 Adenocarcinoma lung FG-236M FB-236M CD35 RLB25 Tonsil FG-431M NA CD35 SP191 Tonsil NA NA CD41 Integrin/Alpha IIb EP178 Spleen FG-732N FB-732N internationalcs@biogenex.com 185 Tissue Control Antibody Clone Recommended Positive Control Control Slides Cat. No Barrier Slides Cat. No CD43 & CD45RA Cocktail MT1 &MB1 Tonsil FG-159M NA CD43 (T Cell,Leukosialin) DFT-1 Tonsil FG-305M NA CD44 (Phagocytic Glycoprotein-1,HCAM) DF1485 Tonsil FG-310M NA CD45 Cocktail (Leukocyte Antigen, LCA) MEM55 + LJ27.9 Tonsil FG-371M FB-371M CD45 (Leukocyte common Antigen,LCA) PD7/26/16&2B11 Tonsil FG-111M NA CD45 (Leukocyte common Antigen,LCA) LJ27.9 Tonsil NA NA CD45RA (B Cell) MB1 Tonsil FG-157M FB-157M CD45RB MEM55 Tonsil FG-320M NA CD45RC (T Cell) MT2 Tonsil FG-156M FB-156M CD45RO (T Cell) UCHL-1 Tonsil FG-113M FB-113M CD48 EP148 Tonsil FG-721N FB-721N CD53 EP179 Tonsil FG-734N FB-734N CD56 (Natural Killer Cell,NCAM) NKH-1 Tonsil NA NA CD57 (Natural Killer Cell) NK-1 Tonsil FG-314M NA CD61 EP2417Y Kidney FG-482N NA CD63 EP211 Melanoma/Prostate Ca. FG-720N FB-720N NA CD66 BY114 Tonsil FG-325M CD68 KP1 Stomach/Lymph Node FG-416M NA CD68 CD68/G2 Histiocytoma FG-549M NA CD71 (Transferrin Receptor) T9 Tonsil NA NA CD71 (Transferrin Receptor) H68.4 Bone Marrow/Fetal Liver NA NA CD74 (B Cell) LN2 Tonsil FG-153M NA CD79a 11E 3 Lymph Node/Tonsil FG-414M NA CD79a EP82 Tonsil/B-cell Lymphoma FG-719N FB-719N CD90 EP56 Thymus FG-733N FB-733N CD95 EP208 Tonsil NA NA CD99 EPR3097Y Ewings Sarcoma FG-518N NA CD99 (MIC2 Antigen) HO36.1.1 Thymus/Tonsil NA NA CD103 EP206 Colon FG-739N FB-739N CD105 4G11 Uterus FG-441M NA CD117 T595 Gastrointestinal Stromal Tumor/ Stomach FG-423M NA CD117 YR145 Gastrointestinal Stromal Tumor/ Stomach FG-465N NA CD146 EP54 Placenta FG-716N FB-716N CD205 EP176 Tonsil FG-737N FB-737N CD227 (MUCIN 1) VU-4H5 Colon/Mucinous Adenocarcinoma FG-534M NA CDw75 (B Cell) LN1 Tonsil FG-152M NA CDX-2 CDX2-88 Colon FG-392M NA CDX-2+CK20+CK7 cocktail CDX2-88+ EPR1622Y+ EPR1619Y Colon/Breast Carcinoma NA NA CDX-2+CK7 cocktail CDX2-88+EPR1619Y Colon/Breast Carcinoma FG-596C FB-596C c-erbB-2 (HER-2/neu) EP1045Y Breast Carcinoma FG-471N NA c-erbB-2 (HER-2/neu) CB11 Breast Carcinoma FG-134M FB-134M c-erbB-2 (HER-2/neu) EP3 Breast Ca. FG-726N FB-726N c-erbB-3 (HER-3) RTJ1/A2 Breast Carcinoma FG-319M NA Chromogranin A LK2H10 Pancreas FG-126M NA 186 www.biogenex.com Tissue Control Antibody Clone Recommended Positive Control Control Slides Cat. No Barrier Slides Cat. No Chromogranin A PHE-5 Pancreas FG-356M FB-356M Claudin 1 Polyclonal Breast Carcinoma/Spleen FG-704P FB-704P Claudin-5 EP224 Vascular/Lung Squamous Ca. FG-718N FB-718N c-myc Protein 9E 10 Adenocarcinoma FG-318M NA Collagen III HWD1.1 Skin FG-167M NA Collagen IV COL-94 Skin NA NA Cyclin D1 Polyclonal Breast Carcinoma FG-447P NA Cyclin D1 EPR2241(IHC)-32 Thyroid Carcinoma FG-474N NA Cyclin E2 EP454Y Glioblastoma FG-519N NA Cytokeratin 4 EP4 Esophagus FG-717N FB-717N Cytokeratin 4 6B10 Tonsil FG-705M FB-705M Cytokeratin 5 EPR1600Y Squamous Cell Carcinoma NA NA Cytokeratin 5 & 6 EPR1600Y & EPR1602Y Squamous Cell Carcinoma FG-588N NA Cytokeratin 5 & Cytokeratin 14 EP24/EP61 Prostate FG-730N FB-730N Cytokeratin 6 EPR1602Y Squamous Cell Carcinoma FG-500N NA Cytokeratin 7 OV-TL12/30 Adenocarcinoma FG-255M FB-255M Cytokeratin 7 EPR1619Y Breast Cancer FG-528N NA Cytokeratin 7 & 8 OV-TL12/30 & C51 Breast Carcinoma FG-587M NA Cytokeratin 8 C-51 Breast Carcinoma FG-142M NA Cytokeratin 8 & 18 5D3 Colon Carcinoma FG-131M FB-131M Cytokeratin 10 DEK-10 Skin FG-201M NA Cytokeratin 13 AE8 Tongue/Esophagus/Tonsil FG-132M NA Cytokeratin 14 LL002 Squamous Cell Carcinoma FG-146M NA Cytokeratin 15 EPR1614Y Squamous Cell Carcinoma NA NA Cytokeratin 16 EP1615Y Squamous Cell Carcinoma NA NA Cytokeratin 17 E27 Basal Cell Carcinoma and Squamous Cell Carcinoma Tissue FG-572M NA Cytokeratin 18 DC-10 Breast Carcinoma FG-143M NA Cytokeratin 19 RCK108 Colon Carcinoma FG-246M NA Cytokeratin 20 IT-Ks20.8 Colon Carcinoma FG-315M NA Cytokeratin 20 EPR1622Y Colon Carcinoma FG-557N NA Cytokeratin Cocktail AE1 and AE3 Skin FG-071M FB-071M Cytokeratin cocktail, Broad Spectrum 34ßE12/C51/AE1 Skin FG-273M NA Cytokeratin cocktail, Broad Spectrum LL002+DEK10+RCK108+OVTL12/30+C11 Breast Carcinoma FG-372M FB-372M Cytokeratin, High MW 34ßE12 Prostate FG-291M FB-291M Cytokeratin, High MW (Basic) AE3 Squamous Cell Carcinoma FG-133M NA Cytokeratin, Low MW AE1 Adenocarcinoma FG-075M FB-075M Cytokeratin, Pan Lu-5 Colon Carcinoma FG-181M NA Cytokeratin, Pan C11 Breast Carcinoma NA NA Cytomegalovirus (CMV) BM204 CMV Infected Cells/Lungs NA NA Desmin D33 Leiomyoma FG-072M FB-072M DOG1 1.1 GIST Tissues FG-570M NA Dystrophin Dys1(Dy4/6D3) Muscle FG-243M NA Dystrophin Dys2(Dy8/6C5) Muscle FG-244M NA E-Cadherin 36 Breast Carcinoma/Colon Carcinoma NA NA E-Cadherin EP6 Colon FG-725N FB-725N internationalcs@biogenex.com 187 Tissue Control Antibody Clone Recommended Positive Control Control Slides Cat. No Barrier Slides Cat. No EGFR Polyclonal Squamous Cell Carcinoma NA NA EGFR(PAN) EP38Y Squamous Cell Carcinoma NA NA Ep-CAM E144 Adenoma FG-489N NA Epithelial Mem Antigen (EMA) E29 Lung FG-057M FB-057M Epithelial Mem Antigen (EMA) Mc5 Breast Cancer FG-182M NA Epithelial-Speciic Antigen MOC-31 Colon Carcinoma FG-316M NA Epstein-Barr Virus Early Antigen 1108-1 EBV Infected Culture Cells FG-222M NA ErbB2/HG2 EP1045Y(R) NA NA NA Estradiol Polyclonal Breast Carcinoma FG-038P NA Estrogen Receptor (ER Alpha) EPR703(2) Breast Carcinoma FG-509M NA Estrogen Receptor 1D5 Breast Carcinoma FG-272M FB-272M Estrogen Receptor EP1(R) NA FG-710N FB-710N Estrogen Receptor (InSite® ER) ER88 Breast Carcinoma FG-368M FB-368M Estrogen Receptor-Beta (ER-β) Polyclonal Breast Carcinoma NA NA Factor VIII-Related antigen F8 2.2.9 Leiomyoma FG-016M FB-016M Factor XIII Subunit A E980.1 Placenta FG-337M NA Factor XIII A EP3372 Bone Marrow FG-516N NA Fascin FCN01 Lymph Node FG-488M NA Fibronectin F14 Placental Tissue FG-569N NA Follicle Stimulating Hormone (FSH) Polyclonal Pituitary NA NA Gastrin Polyclonal Gastrin FG-019P NA GCDFP-15 EP1582Y Breast Carcinoma FG-481N NA Glial Fibrillary Acidic Protein (GFAP) GA-5 Cerebellum FG-020M FB-020M Glial Fibrillary Acidic Protein (GFAP) Polyclonal Cerebellum FG-020P NA Glomerular Epithelial Protein 1 (GLEPP-1) 5C11 Kidney NA NA Glucagon Polyclonal Pancreas FG-039P NA Glut-1 SPM498 Esophagus Carcinoma/ Squamous Carcinoma FG-505M NA Glutathione S-Transferase pi (GST Pi) Polyclonal Breast FG-249P NA Glypican-3 (GPC3) GPC3-88 Hepatocellular Carcinoma FG-539M NA Granulocyte BM-2 Hodgkin FG-210M NA Growth Hormone (Hgh) Polyclonal Pituitary NA NA Heat Shock Protein (HSP-70) BRM-22 Breast Carcinoma FG-289M NA Heat Shock Protein 27 (HSP 27) G3.1 Breast Carcinoma FG-171M FB-171M Helicobacter Pylori Polyclonal H. Pylori Infected Stomach Tissue FG-442P NA Hemoglobin A Polyclonal Placenta FG-021P NA Hepatitis B Virus Core Antigen (HBcAg) Polyclonal Infected Liver FG-082P NA Herpes Simplex Virus Type I (HSV I) Polyclonal Infected Lung NA NA Herpes Simplex Virus Type II (HSV II) Polyclonal Infected Lung NA NA HGH Polyclonal Placenta/Pituitary Gland FG-707P FB-707P HLA-DR LN3 Tonsil FG-154M NA HSA HSA/E8 Liver Tissues FG-550M NA Human Chorionic Gonadotropin (hCG) Beta M94138 Placenta NA NA IgA Polyclonal Tonsil NA NA IgD Polyclonal Tonsil FG-440P NA IgG IgG88 Tonsil FG-367M NA IgG Polyclonal Tonsil FG-050P NA IgM IgM88 Tonsil FG-366M NA IgM Polyclonal Tonsil FG-427P FB-427P 188 www.biogenex.com Tissue Control Antibody Clone Recommended Positive Control Control Slides Cat. No Barrier Slides Cat. No Inhibin-Alpha R1 Ovary FG-446M NA Insulin HB125 Pancreas FG-029M NA Insulin EP125 Pancreas/Insulinoma FG-735N FB-735N J Chain JC88 Tonsil NA NA Kappa Light Chain L1C1 Tonsil FG-048M NA Kappa Light Chain K88 Tonsil FG-369M FB-369M Ki-67 K-2 Tonsil FG-410M FB-410M Ki-67 EPR3611 Breast Carcinoma/Tonsil FG-527N NA Ki-67 EP5 Tonsil FG-727N FB-727N Ki-67 and Lambda light chain cocktail K-2 and Polyclonal Tonsil Tissues FG-562C NA Ki-67 Antigen,Proliferating Cell MIB-1 Lymphoma FG-297M NA Ki-67 Antigen,Proliferating Cell Ki88 Tonsil FG-370M FB-370M Lambda Light Chain Polyclonal Tonsil FG-049P NA Lambda Light Chain EP172 Tonsil/B-cell Lymphoma FG-715N FB-715N Laminin Polyclonal Bronchus FG-078P FB-078P Luteinizing Hormone (LH) Polyclonal Pituitary NA NA Lysozyme Polyclonal Tonsil/Lymph Node FG-024P FB-024P Macrophage LN5 Liver Tissues FG-165M NA Mast Cell Tryptase AA1 Skin FG-419M FB-419M Melan-A (MART-1) A103 Melanoma FG-361M FB-361M Melanoma HMB45 Melanoma FG-001M FB-001M Melanoma gp100 gp100/D5 Melanoma Tissue FG-536M NA Melanoma-Associated Antigen NKI/C3 Melanoma FG-077M FB-077M Mesothelin 5B2 Ovary Cyst Adenocarcinoma FG-433M NA MiTF MiTF/A13 Melanoma Tissue FG-554M NA Mitochondrial Antigen 113-1 Breast Carcinoma NA NA MLH1 ES05 Colon Tissue FG-703M FB-703 MMP-9 EP1255Y Bone Marrow FG-504N NA MSH2 SP46 Colon NA NA MSH6 2D4B5 Colon Carcinoma FG-454M NA MUC4 1G8 Colon Carcinoma FG-455M NA MUC5AC 45M1 Stomach Mucosa/Gastrointestinal Tissue FG-456M NA Mucin 1 (MUC1) EPR1023 Breast Carcinoma FG-564N NA Mucin 2 (MUC2) CCP58 Colon Carcinoma NA NA Muscle Actins Actin 88 Cocktail Muscle NA NA Myelin Basic Protein MBP88 Cerebellum NA NA Myeloid speciic Antigen BM-3 Bone Marrow NA NA Myeloid speciic Antigen BM-1 Lymph node/Tonsil/Bone Marrow FG-164M NA Myeloperoxidase (MPO) Polyclonal Spleen FG-496P NA Myf4 LO26 Rhabdomyosarcoma FG-432M NA Myoglobin MG-1 Muscle FG-012M NA Myoglobin Polyclonal Muscle FG-012P NA Myosin Heavy Chains,Smooth Muscle SMMS.1 Breast Carcinoma NA NA Myosin,Skeletal Muscle MY-32 Muscle FG-109M NA Napsin A IP64 Lung Adenocarcinoma FG-701M FB-701M N-Cadherin EPR1792Y Colon Carcinoma Tissue FG-565N NA internationalcs@biogenex.com 189 Tissue Control Antibody Clone Recommended Positive Control Control Slides Cat. No Barrier Slides Cat. No Neuroilament NE-14 Nerve FG-073M FB-073M Neuron Speciic Enolase (NSE) MIG-N3 Pancreas/Nerve FG-055M FB-055M NGF Receptor EP1039Y(R) Neuroblastoma NA NA NGFR EP31 Pancreas/Melanoma FG-738N FB-738N Nuclear Ribonucleoprotein 58-15 Spleen FG-230M NA Oct 2 EPR542 Tonsil tissues FG-558N NA Oct 4 EP143 Seminoma in Testis FG-724N FB-724N Osteonectin OST1 Osteosarcoma NA NA p105 Proliferation-Associated Nuclear Antigen 2B3 Tonsil NA NA p16 (INK4a) G175-405 Sqamous Cell Carcinoma FG-540M NA p16+Ki67 cocktail G175-405 +EPR3611 Cervical Carcinoma FG-601C FB-601C p21/WAF1 4D10 Melanoma FG-434M NA p27 (Kip1) DCS72 Breast Carcinoma NA NA p27 (Kip1) Y236 Breast Carcinoma FG-492N NA p34cdc2 (Cyclin Dependent Kinase) POH-1 Tonsil FG-301M NA P504S (AMACR) RBT-AMACR Prostate Carcinoma FG-538N NA P504S (AMACR) 13H4 Prostate Carcinoma FG-449N NA p53 EP9 Breast Ca. FG-728N FB-728N p53 Protein BP53-12-1 Breast Carcinoma FG-195M FB-195M p53 Protein DO7 Breast Carcinoma FG-239M FB-239M p53 Protein 1801 Breast Carcinoma FG-240M NA p63 Protein 4A4(M) Prostate Carcinoma FG-418M FB-418M A40010 Benign Prostate Hyperplasia(BPH) and Carcinoma Prostate FG-532M NA PAP Papillomavirus Type 16 (HPV-16) CamVir-1 HPV-16 Infected Cells/Tissue NA NA PAX-5 ZP007 Lymph Node FG-457M NA Paxillin (N-Term) Y113 Breast Carcinoma FG-521N NA PDCD4 EPR3432 Breast Carcinoma FG-524N NA NA PDGF Receptor-ß Y92 GIST FG-463N PGP9.5 3D9 Brain/Small Intestine FG-736M FB-736M PIN4 4A4+34ßE12+13H4 Prostate Carcinoma FG-448M NA Placental Alkaline Phosphatase (PLAP) PL8-F6 Placenta FG-228M FB-228M Placental Lactogen (hPL) Polyclonal Placenta FG-040P NA Platelet-Derived Growth Factor (PDGF) PDGF88 Squamous NA NA Platelet-Derived Growth Factor (PDGF) Polyclonal Squamous NA NA PMS2 EPR3947 Colon Carcinoma FG-577N NA Progesterone Receptor EP2(R) NA FG-711N FB-711N Progesterone Receptor 1A6 Breast Carcinoma FG-172M NA Progesterone Receptor (InSite PR) PR88 Breast Carcinoma FG-328M FB-328M Prolactin ME.121 Pituitary NA NA Proliferating Cell Nuclear Antigen (PCNA) PC10 Colon Carcinoma FG-252M FB-252M Prostate Speciic Acid Phosphatase (PSAP) B01-94-21M-NA Prostate Hyperplasia FG-013M FB-013M Prostate Speciic Antigen (PSA) ErPr-8 Prostate Carcinoma NA FB-014M pS2 Estrogen Inducible Protein PS2.1 Breast Carcinoma FG-190M FB-190M PSMA EP192 Prostate Ca. FG-714N FB-714N RCC (Renal Cell Carcinoma) RCC-26 Kidney,Renal Cell Carcinoma FG-543M NA S100 beta EP32 Prostate Ca. FG-713N FB-713N S-100 Protein 15E2E2 Melanoma FG-058M FB-058M S-100 Protein Polyclonal Melanoma FG-058P NA ® 190 www.biogenex.com Tissue Control Antibody Clone Recommended Positive Control Control Slides Cat. No Barrier Slides Cat. No S100P EP186 Placenta/Pancreatic Adeno Ca. FG-712N FB-712N Sarcomeric Actin ZMSA-5 Muscle FG-511M NA Secretin Polyclonal Stomach FG-067P NA SOX2 EPR3131 Sqamous Cell Carcinoma FG-579N NA Stat 5-Alpha (C-term) E289 Breast Carcinoma FG-480N NA Substance P Polyclonal Hypothalamus FG-069P NA Survivin EP2880Y TCC Bladder Tissue/Bladder Carcinoma FG-503N NA Synaptophysin Snp88 Pancreas FG-363M FB-363M Tau Tau-2 Cerebellum FG-412M FB-412M Tau Tau-5 Brain FG-459M FB-459M Terminal Deoxynucleotidyl Transferase (TdT) TdT88 Thymus NA NA Terminal Deoxynucleotidyl Transferase (TdT) Polyclonal Thymus FG-373P NA TFPU.1 EPR3159Y Lymphoma FG-520N NA Thyroglobulin 2H11 Follicular Adenoma FG-032M NA Thyroid Stimulating Hormone (TSH) 5404 Follicular Adenoma NA NA Thyroid Stimulating Hormone (TSH) Polyclonal Follicular Adenoma NA NA Thyroid Transcription Factor (TTF-1) 8G7G3/1 Lung FG-397M NA Thyroxine D5 Thyroid FG-034M NA Topo 2 Alpha EP1102Y Thyroid Carcinoma/Breast Carcinoma) FG-576N NA Toxoplasma gondii Polyclonal Infected Cells/Tissues FG-125P NA Transferrin HT1/13.6.3 Liver/Muscle FG-025M NA Transforming Growth Factor (TGF),Alpha TGF88 Breast Carcinoma NA NA TTF-1 and Cytokeratin 5/6 cocktail 8G7G3/1, EPR1600Y,EPR1602Y Lung/Squamous FG-563C NA TTF-1+CK20+CK7 cocktail 8G7G3/1+EPR1622Y +EPR1619Y Lung Carcinoma NA NA TTF-1+GCDFP-15 cocktail 8G7G3/1+EP1582Y Lung Carcinoma FG-604C NA Tumor-Associated Glycoprotein (TAG-72) B72.3 Breast Carcinoma FG-054M FB-054M Tumor-Associated Glycoprotein (TAG-90 BCA) B6.2 Breast Carcinoma FG-005M NA Tyrosinase Ty/G5 Melanoma FG-535M NA VEGF Polyclonal Angiosarcoma FG-483P NA Villin ID2C3 Small Intestine FG-525M NA Vimentin V9 Skin/Leiomyoma FG-074M FB-074M Vimentin SP20 Leiomyoma FG-566N NA Vimentin,Non-Hematopoietic LN6 Submucosal Vascular Endothelial cells/Leiomyoma FG-163M NA VIP Polyclonal Small Intestine FG-530P NA Wilm’s Tumor CAN-R9(IHC)-56-2 Wilm’s Tumor FG-584N NA ZAP-70 ZAP70-C3 Tonsil and Reactive Lymph Node FG-544M NA ZAP-70 YE291 Tonsil FG-466N NA NOTE: The list for positive control slides is constantly being updated, depending upon tissue availability. Please call 1(800) 421-4149 for availability or visit our website at www.biogenex.com internationalcs@biogenex.com 191 Tissue Control 192 www.biogenex.com Index Alphabetical Product Name Index Description Clone Cat. No Pack Size Page Aberrant Endothelial Cell 4A11 Acid Fast Blue Stain Kit - AM382-5M, AX382-YCD, MU382-UC 6ml, 16 ml, 1ml 11, 43, 51 SS025-50X 50 Slides 168, 173 ACTH AH26 AM487-5M, AM487-10M, AX487-YCD, MU487-UC 6 ml, 10 ml, 16 ml, 1 ml 11, 23, 43, 51, 184 Actin, Muscle-Specific HHF35 AM090-5M, AM090-10M, AX090-YCD, MU090-UC 6ml, 10ml, 16 ml, 1ml 11, 23, 43, 51, 184 Actin, Smooth Muscle 1A4 AM128-5M, AM128-10M, AX128-YCD, MU128-UC 6ml, 10ml, 16 ml, 1ml 11, 23, 43, 51, 184 Adenovirus A62020069P AM059-5ME, AX059-YCDE, MU059-UCE 6ml, 10 ml, 1ml 11, 43, 52 AEC Chromogen - HK139-06K 6ml 178 AEC Chromogen - HK139-50KE 50ML 178 AEC Substrate Kit, Conc - HK129-5KE 2500 Slides 178 AEC Substrate Pack Kit - HK092-5K 250 Slides 178 AFB Green - SS059-50X 50 Slides 168, 173 168, 173, 174 A Alcian Blue PAS Stain - SS026-50X, SS026-YAK 50 Slides, 50 Slides Alcian Blue Stain - SS027-50X, SS027-YAK 50 Slides, 50 Slides 168, 173, 174 Alizarin Red Stain - SS052-50K 50 Slides 168, 174 Alpha-1-Antichymotrypsin a1A88 AM388-5M, AX388-YCD, MU388-UC 6ml, 16 ml, 1ml 11, 43, 52 Alpha-1-Antitrypsin Polyclonal AR015-5R, AW015-YCD, PU015-UP 6ml, 16 ml, 1ml 11, 43, 52, 184 Alpha-Actinin JLN20 AM097-5M, AX097-YCD, MU097-UC 6ml, 16 ml, 1ml 11, 43, 52, 184 Alpha-Fetoprotein (AFP) C3 AM008-5M, AM008-10M, AX008-YCD, MU008A-UC 6ml, 10ml, 16 ml, 1ml 11, 23, 43, 53, 184 Alpha-Tubulin DM-1A MU121-UC 1ml 43, 53, 184 ALU Probe II - PR026-100E, PR026-YADE 0.650 ml, 0.650 ml 19, 150, 151 Androgen Receptor F39.4.1 AM256-2ME, AM256-10ME, AX256-YCDE, MU256UCE 3ml, 10ml, 16ml,1ml 11, 23, 43, 53, 184 Antigen Retrieval AR-10 (10X) - HK057-5KE 100 ml 37 Antigen Retrieval AR-10 RTU - HK058-5K, HK058-20K 250 ml, 1L 37 Antigen Retrieval Citra (10X) - HK086-5K, HK086-9K 100 ml, 500 ml 36 Antigen Retrieval Citra Plus (10X) - HK080-5K, HK080-9K 100 ml, 500 ml 36 Antigen Retrieval Citra Plus RTU - HK081-5KE, HK081-20KE 250 ml, 1L 36 Antigen Retrieval Citra RTU - HK087-5KE, HK087-20KE 250 ml, 1L 36 Apolipoprotein E EP1373Y AN567-5M, AN567-10M, AX567-YCD, NU567-UC 6 ml, 10 ml, 16 ml, 1 ml 11, 23, 43, 53, 184 Aqueous Mounting Medium - HK099-5K 15ml 180 Auto DAB kit for Xmatrx Elite - XT150-YCDE 200 slides 18 AutoFISH Pretreatment Reagent - HK983-YRXE 500 ml 163 AutoFISH Protease 1 Buffer - HK981-YRXE 500 ml 163 AutoFISH Protease 2 Buffer - HK982-YRXE 500 ml 163 AutoFISH Reagent A - HK972-YRXE 500 ml 163 AutoFISH Reagent B - HK973-YRXE 500 ml 163 28, 177 Avidin Biotin Blocking Kit - HK102-5K, HK102-10K, HK102-20X 60 Slides, 100 Slides, 200 Slides Azure A satin - SS038-50X, SS038-YAK 50 Slides, 50 Slides 169, 173, 174 B Cell MB2 AM158-5M, AM158-10M, AX158-YCD, MU158-UC 6ml, 10ml, 16 ml, 1ml 11, 23, 43, 54, 184 B Lymphocyte Antigen 36 (BLA.36) A27-42 AM231-5M, AM231-10M, AX231-YCD, MU231-UC 6ml, 10ml, 16 ml, 1ml 11, 23, 43, 54, 184 B Barrier Slides (25x25mm) - XT108-CL, XT108-SL 1440 Slides, 72 slides 20, 22 Barrier Slides, 18X18mm, for Xmatrx - XT128-CL, XT128-SL 1440 Slides, 72 slides 20, 22 Barrier Slides, 2 Zones, for Xmatrx - XT114-CL, XT114-SL 1440 Slides, 72 slides 20, 22 Barrier Slides, 25X40mm, for Xmatrx - XT134-CL, XT134-SL 1440 Slides, 72 slides 20, 22, 29, 181 194 www.biogenex.com Alphabetical Product Name Index Description Clone Cat. No Pack Size Page Bax Protein Polyclonal AR347-5R, AW347-YCD, PU347-UP 6ml, 16 ml, 1ml 11, 43, 54 Bcl-2 E17 AN541-5M, AN541-10M, AX541-YCD, NU541-UC 6 ml, 10 ml, 16 ml, 1 ml 11, 23,43, 54, 184 Bcl-2 EP36 AN723-5M, AN723-10M, AY723-YCD, NU723-UC 6 ml, 10 ml, 16ml, 1ml 11, 23, 43, 55, 184 Bcl-2 Oncoprotein bcl-2/100 AM287-5M, AM287-10M, AX287-YCD 6ml, 10ml, 16 ml 11, 23, 43, 55, 184 Bcl-6 LN22 AM708-5M, AM708-10M, AY708-YCD, MU708-UC 6 ml, 10 ml, 16ml, 1ml 11, 23, 43, 55 6 ml, 10 ml, 16 ml, 1ml 11, 23, 43, 55, 184 Bcl-x E18 AN462-5M, AN462-10M, AX462-YCD, NU462-UC Beta Actin - PR1055-100E, PR1055-YADE 0.650 ml, 0.650 ml 19, 150, 151 11, 23, 43, 56 ,184 Beta-Catenin E247 AN510-5M, AN510-10M, AX510-YCD, NU510-UC 6 ml, 10 ml, 16 ml, 1 ml Beta-Human Chorionic Gonadotropin (hCG) M94138 AM395-5M, AX395-YCD, MU395-UC 6ml, 16 ml, 1ml 15, 48, 103 Beta-Tubulin DM-1B MU122-UC 1ml 43, 56, 184 Beta-Tubulin II JDR3B8 MU176-UC 1ml 43, 56, 184 Beta-Tubulin III SDL3D10 MU177-UC 1ml 43, 56, 184 Beta-Tubulin IV ONS1A6 MU178-UC 1ml 43, 57, 184 bFGF (Basic Fibroblast Growth Factor) bFGF88 AM359-5M, AX359-YCD 6ml, 16 ml 11, 43, 57 Bielschowsky's Silver Stain - SS055-50X, SS055-YAK 50 Slides, 50 Slides 169, 173, 174 Blocking Reagent II Goat Serum - HK112-5K, HK112-9KE 6ml, 50 ml 177 Blocking Reagent II Rabbit Serum - HK114-5K 6ml 177 Blood Group Antigen Lewis A 7LE MU303-UC 1ml 43, 57, 184 Blood Group Antigen Lewis B 2-25LE MU304-UC 1ml 43, 57, 184 BOB1 SP92 AN731-5ME, AN731-10ME, AY731-YCDE, NU731UCE 6ml, 10ml, 16ml, 1ml 11, 23, 43, 58 BRCA1 Protein Polyclonal AR345-5R, AW345-YCD, PU345-UP 6ml, 16 ml, 1ml 11, 43, 55 Breast Cancer Antigen BCA 225 CU18 AM135-5M, AM135-10M, AX135-YCD 6ml, 10ml, 16 ml 11, 23, 43, 58, 184 CA125 Ov185:1 AM429-5M, AM429-10M, AX429-YCD, MU429-UC 6ml, 10ml, 16 ml, 1ml 11, 23, 43, 59, 184 CA19-9 C241:5:1:4 AM424-5M, AM424-10M, AX424-YCD, MU424-UC 6ml, 10ml, 16 ml, 1ml 11, 23, 43, 58, 184 Caldesmon E89 AN507-5M, AN507-10M, AX507-YCD, NU507-UC 6 ml, 10 ml, 16 ml, 1 ml 11, 23, 43, 59, 184 Caldesmon, High MW, Smooth muscle h-CD AM332-5M, AX332-YCD, MU332-UC 6ml, 16 ml, 1ml 11, 43, 59 Calponin CALP AM333-5M, AX333-YCD, MU333-UC 6ml, 16 ml, 1ml 11, 43, 59, 184 11, 23, 43, 60, 184 C Calponin-1 (C-term) EP798Y AN502-5M, AN502-10M, AX502-YCD, NU502-UC 6 ml, 10 ml, 16 ml, 1 ml Calretinin 2E7 AM583-5M, AM583-10M, AX583-YCD, MU583-UC 6 ml, 10 ml, 16 ml, 1 ml 11, 23, 43, 60, 184 Calretinin Polyclonal AR413-2R, AR413-5R,AR413-10R, AW413-YCD, PU413-UP 3ml, 6ml, 1ml, 10ml, 16 ml, 1ml 11, 23, 60, 184 Cap for Optimiser Vials (24) - XT022-CP 24/pk 28 16, 23, 44, 61, 185 Catenin (p120) YE372 AN523-5M, AN523-10M, AX523-YCD, NU523-UC 6 ml, 10 ml, 16 ml, 1 ml Catenin Delta 1 (p120) Polyclonal AR706-5R, AR706-10R, AW706-YCD, PU706-UP 6ml, 10 ml, 16 ml, 1ml 11, 23, 44, 61, 185 Cathepsin D C15 AM467-5M, AX467-YCD, MU467-UC 6 ml, 16 ml, 1 ml 11, 44, 62 11, 23, 44, 62, 185 CD1a O10 AM490-5M, AM490-10M, AX490-YCD, MU490-UC 6 ml, 10 ml, 16 ml, 1 ml CD2 AB75 AM438-5M,AX438-YCD, MU438-UC 6ml,16ml, 1 ml 11, 44, 62, 185 CD3 (T Cell) UCHT1 AM258-5M, AX258-YCD, MU258-UC 6ml, 16 ml, 1ml 12, 44, 63 CD3 (T Cell) PS1 AM322-5M, AM322-10M, AX322-YCD, MU322-UC 6ml, 10ml, 16 ml, 1ml 12, 23, 44, 63, 185 CD3 and CD20 cocktail Mouse L26 + Rabbit EP449E AC559-5M, AC559-10M, AC559-YCD 6 ml, 10 ml, 16 ml 12, 23, 145, 185 internationalcs@biogenex.com 195 Alphabetical Product Name Index Description Clone Cat. No Pack Size Page 12, 23, 44, 62, 185 CD3 Epsilon EP449E AN477-5M, AN477-10M, AX477-YCD, NU477-UC 6 ml, 10 ml, 16 ml, 1 ml CD4 4B12 AM421-5M, AM421-10M, AX421-YCD, MU421-UC 6ml, 10ml, 16 ml, 1ml 12, 23, 44, 63, 185 CD4 EP204 AN722-5M, AN722-10M, AY722-YCD, NU722-UC 6 ml, 10 ml, 16mL, 1mL 12, 23, 44, 63, 185 CD4 + C8 Cocktail Mouse 4B12 + Rabbit EP1150Y AC595-5M, AC595-10M, AC595-YCD 6 ml, 10 ml, 16 ml 12, 23, 145 CD5 4C7 AM430-5M, AM430-10M, AX430-YCD, MU430-UC 6ml, 10ml, 16 ml, 1ml 12, 23, 44, 64, 185 CD5 EP2952 AN512-5M, AN512-10M, AX512-YCD, NU512-UC 6 ml, 10 ml, 16 ml, 1 ml 12, 23, 44, 64, 185 CD7 LP15 AM702-5M, AM702-10M, AY702-YCD, MU702-UC 6ml, 10ml, 16 ml, 1ml 12, 23, 44, 64, 185 CD8 (T Cell,Suppressor/Cytotoxic) T8 AM261-5M, AX261-YCD, MU261-UC 6ml, 16 ml, 1ml 12, 44, 65 CD8 1A5 AM422-5M, AM422-10M, AX422-YCD, MU422-UC 6ml, 10ml, 16 ml, 1ml 12, 23, 44, 64, 185 CD8 SP16 AN740-5M, AN740-10M, AY740-YCD, NU740-UC 6ml , 10ml, 16ml, 1ml 12, 23, 44, 65 CD8 (C-Term) EP1150Y AN513-5M, AN513-10M, AX513-YCD, NU513-UC 6 ml, 10 ml, 16 ml, 1 ml 12, 23, 44, 65, 185 CD10 56C6 AM451-5M, AM451-10M, AX451-YCD, MU451-UC 6 ml, 10 ml, 16 ml, 1 ml 12, 23, 44, 65, 185 CD10 and Cyclin D1 cocktail Mouse 56C6 + Rabbit EPR2241 (IHC)-32 AC561-5M, AC561-10M, AC561-YCD 6 ml, 10 ml, 16 ml 12, 24, 145, 185 CD11b (C3bi Receptor) M01 AM270-5M, AX270-YCD 6ml, 16 ml 12, 44, 66 12, 24, 44, 66, 185 CD11b/ITAM EPR1344 AN546-5M, AN546-10M, AX546-YCD, NU546-UC 6 ml, 10 ml, 16 ml, 1 ml CD11c EP1347Y AN555-5M, AN555-10M, AX555-YCD, NU555-UC 6 ml, 10 ml, 16 ml, 1 ml 12, 24, 44, 66, 185 CD14 EPR3653 AN515-5M, AN515-10M, AX515-YCD, NU515-UC 6 ml, 10 ml, 16 ml, 1 ml 12, 24, 44, 65, 185 CD15 BRA4F1 AM302-5M, AM302-10M, AX302-YCD, MU302-UC 6ml, 10ml, 16 ml, 1ml 12, 24, 44, 67, 185 CD16 2H7 AM437-5M, AX437-YCD, MU437-UC 6ml, 16 ml, 1ml 12, 44, 67, 185 CD19 EP169 AN729-5M, AN729-10M, AY729-YCD, NU729-UC 6 ml, 10 ml, 16ml, 1ml 12, 24, 44, 67, 185 CD20 CD20/C23 AM537-5M, AM537-10M, AX537-YCD, MU537-UC 6 ml, 10 ml, 16 ml, 1 ml 12, 24, 44, 67, 185 CD20 (B Cell) L-26 AM238-5M, AM238-10M, AX238-YCD, MU238A-UC 6ml, 10ml, 16 ml, 1ml 12, 24, 44, 185 CD20 (B Cell) B1 AM265-5M, AX265-YCD, MU265-UC 6ml, 16 ml, 1ml 12, 44, 68 12, 24, 44, 68, 185 12, 44, 68 CD21 EP3093 AN497-5M, AN497-10M, AX497-YCD, NU497-UC 6 ml, 10 ml, 16 ml, 1 ml CD21 (B Cell) B2 AM266-5M, AX266-YCD 6ml, 16 ml CD22 FPC1 AM439-5M, AX439-YCD, MU439-UC 6ml, 16 ml, 1ml 12, 44, 69, 185 12, 24, 44, 69, 185 12, 24, 44, 69, 185 CD23 Polyclonal AR460-5R, AR460-10R, AW460-YCD, PU460-UP 6 ml, 10 ml, 16 ml, 1 ml CD25 4C9 AM453-5M, AM453-10M, AX453-YCD, MU453-UC 6 ml, 10 ml, 16 ml, 1 ml CD29 JB1a AM298-5M, AX298-YCD, MU298-UC 6ml, 16 ml, 1ml 12, 44, 69, 185 CD30 (Ki-1 Antigen) HRS-4 AM351-5M, AM351-10M, AX351-YCD, MU351-UC 6ml, 10ml, 16 ml, 1ml 12, 24, 44, 70 CD30 (Ki-1 Antigen) Ber-H2 AM327-5M, AM327-10M, AX327-YCD 6ml, 10ml, 16 ml 12, 24, 44, 70, 185 CD31 JC/70A AM232-5M, AM232-10M, AX232-YCD 6ml, 10ml, 16 ml 12, 24, 44, 70, 185 CD31 9G11 AM241-5M, AX241-YCD, MU241-UC 6ml, 16 ml, 1ml 12, 44, 70, 185 CD34 (Endothelial Cell) QBend/10 AM236-5M, AM236-10M, AX236-YCD, MU236-UC 6ml, 10ml, 16 ml, 1ml 12, 24, 44, 71, 185 CD35 RLB25 AM431-5M, AM431-10M, AX431-YCD, MU431-UC 6ml, 10ml, 16 ml, 1ml 12, 24, 44, 71, 185 CD35 SP191 AN741-5M, AN741-10M, AY741-YCD, NU741-YCD 6ml , 10ml, 16ml, 1ml 12, 24, 44, 71 196 www.biogenex.com Alphabetical Product Name Index Description CD41/Integrin Alpha IIb Clone Cat. No Pack Size Page EP178 AN732-5ME, AN732-10ME, AY732-YCDE, NU732UCE 6ml , 10ml, 16ml, 1ml 12, 24, 44, 71 CD43 & CD45RA Cocktail MT1 & MB1 AM159-5M, AX159-YCD 6ml, 16 ml 12, 44, 72, 186 CD43 (T Cell, Leukosialin) DFT-1 AM305-5M, AX305-YCD, MU305-UC 6ml, 16 ml, 1ml 12, 44, 72, 186 CD44 (Phagocytic Glycoprotein-1,HCAM) DF1485 AM310-5M, AX310-YCD, MU310-UC 6ml, 16 ml, 1ml 12, 45, 72, 186 CD45 PD7/26/16 & 2B11 AM111-5M, AM111-10M, AX111-YCD 6ml, 10ml, 16 ml 12, 24, 45, 72, 186 CD45 (Leukocyte common Antigen,LCA) LJ27.9 AM338-5M, AX338-YCD, MU338-UC 6ml, 16 ml, 1ml 12, 45, 73 CD45 Cocktail (Leukocyte Antigen, LCA) MEM55+LJ27.9 AM371-5M, AM371-10M, AX371-YCD, MU371-UC 6ml, 10ml, 16 ml, 1ml 12, 24, 45, 73, 186 CD45RA (B Cell) MB1 AM157-5M, AM157-10M, AX157-YCD 6ml, 10ml, 16 ml 12, 24, 45, 73, 186 CD45RB MEM55 AM320-5M, AX320-YCD, MU320-UC 6ml, 16 ml, 1ml 12, 45, 73, 186 CD45RC (T Cell) MT2 AM156-5M, AX156-YCD, MU156-UC 6ml, 16 ml, 1ml 12, 45, 74, 186 CD45RO (T Cell) UCHL-1 AM113-5M, AM113-10M, AX113-YCD, MU113-UC 6ml, 10ml, 1ml, 16 ml 12, 24, 45, 74, 186 CD48 EP148 AN721-5ME, AN721-10ME, AY721-YCDE, NU721UCE 6ml , 10ml, 16ml, 1ml 13, 24, 45, 74 CD53 EP179 AN734-5M, AN734-10M, AY734-YCD, NU734-UC 6 ml, 10 ml, 16mL, 1mL 13, 24, 45, 74, CD56 (Natural Killer Cell,NCAM) NKH-1 AM268-5M, AX268-YCD, MU268-UC 6ml, 16 ml, 1ml 13, 45, 75 CD57 (Natural Killer Cell) NK-1 AM314-5M, AX314-YCD, MU314-UC 6ml, 16 ml, 1ml 13, 45, 75, 186 CD61 EP2417Y AN482-5M, AN482-10M, AX482-YCD, NU482-UC 6 ml, 10 ml, 16 ml, 1 ml 13, 24, 45, 75, 186 CD63 EP211 AN720-5ME, AN720-10ME, AY720-YCDE, NU720UCE 6ml , 10ml, 16ml, 1ml 13, 24, 45, 75, 186 CD66 BY114 AM325-5M, AX325-YCD 6ml, 16 ml 13, 45, 76, 186 13, 24, 45, 76, 186 13, 24, 45, 76, 186 CD68 CD68/G2 AM549-5M, AM549-10M, AX549-YCD, MU549-UC 6 ml, 10 ml, 16 ml, 1 ml CD68 KP1 AM416-5M, AM416-10M, AX416-YCD, MU416-UC 6ml, 10ml, 16 ml, 1ml CD71 (Transferrin Receptor) T9 AM269-5M, AX269-YCD 6ml, 16 ml 13, 45, 76 CD71 (Transferrin Receptor) H68.4 AM354-5M, AX354-YCD, MU354-UC 6ml, 16 ml, 1ml 13, 45, 77 CD74 (B Cell) LN2 AM153-5M, AX153-YCD, MU153-UC 6ml, 16 ml, 1ml 13, 45, 77, 186 CD79a 11E 3 AM414-5M, AM414-10M, AX414-YCD, MU414-UC 6ml, 10ml, 16 ml, 1ml 13, 24, 45, 77, 186 CD79a EP82 AN719-5M, AN719-10M, AY719-YCD, NU719-UC 6 ml, 10 ml, 16mL, 1mL 13, 24, 45, 77, 186 CD90 EP56 AN733-5M, AN733-10M, AY733-YCD, NU733-UC 6 ml, 10 ml, 16mL, 1mL 13, 24, 45, 78, 186 CD99 (MIC2 Antigen) HO36.1.1 AM355-5M, AM355-10M, AX355-YCD, MU355-UC 6ml, 10ml, 16 ml, 1ml 13, 24, 45, 78 13, 24, 45, 78, 186 13, 24, 45, 79 CD99 EPR3097Y AN518-5M, AN518-10M, AX518-YCD, NU518-UC 6 ml, 10 ml, 16 ml, 1 ml CD103 EP206 AN739-5ME, AN739-10ME, AY739-YCDE, NU739UCE 6ml, 10ml, 16mL, 1mL CD105 4G11 AM441-5M, AX441-YCD, MU441-UC 6ml, 16 ml, 1ml 13, 45, 79, 186 CD117 YR145 AN465-5M, AN465-10M, AX465-YCD, NU465-UC 6 ml, 10 ml, 16 ml, 1ml 13, 24, 45, 79, 186 CD117 T595 AM423-5M, AM423-10M, AX423-YCD, MU423-UC 6ml, 10ml, 16 ml, 1ml 13, 24, 45, 79, 186 CD146 EP54 AN716-5M, AN716-10M, AY716-YCD, NU716-UC 6 ml, 10 ml, 16mL, 1mL 13, 24, 45, 80, 186 CD205 EP176 AN737-5ME, AN737-10ME, AY737-YCDE, NU737UCE 6ml, 10ml, 16mL, 1mL 13, 24, 45, 80 CD227 (MUCIN 1) VU-4H5 AM534-5M, AM534-10M, AX534-YCD, MU534-UC 6 ml, 10 ml, 16 ml, 1 ml 13, 26, 45, 80, 186 CDw75 (B Cell) LN1 AM152-5M, AX152-YCD, MU152-UC 6ml, 16 ml, 1ml 13, 45, 80, 186 6ml, 10ml, 16 ml, 1ml 13, 24, 45, 81, 186 CDX-2 internationalcs@biogenex.com CDX2-88 AM392-5M, AM392-10M, AX392-YCD, MU392A-UC 197 Alphabetical Product Name Index Description Clone Cat. No Pack Size Page CDX-2+CK20+CK7 cocktail Mouse 7C7-D4 + Rabbit EPR1622Y+ EPR1619Y AC597-5M, AC597-10M, AC597-YCD 6 ml, 16 ml 13, 24, 147 CDX-2+CK7 cocktail Mouse 7C7-D4 + Rabbit EPR1619Y AC596-5M, AC596-10M, AC596-YCD 6 ml, 16 ml 13, 24, 145, 186 Carcinoembryonic Antigen (CEA) B01-94-11M-P AM009-5M, AM009-10M, AX009-YCD, MU009-UC 6ml, 10ml, 16 ml, 1ml 11, 23, 43, 60, 185 Carcinoembryonic Antigen (CEA) CEA88 AM365-5M, AM365-10M, AX365-YCD, MU365-UC 6ml, 10ml, 16 ml, 1ml 13, 23, 43, 61, 185 Carcinoembryonic Antigen (CEA) Polyclonal AR009-5R, AR009-5RSG, AW009-YCD 6ml, 1ml, 16 ml 11, 43, 61, 185 c-erbB-2 (HER-2/neu) CB11 AM134-5ME, AM134-10ME, AX134-YCDE, MU134UCE 6ml, 10ml, 1ml 13, 24, 45, 81 c-erbB-3 (HER-3) RTJ1/A2 AM319-5M, AX319-YCD, MU319-UC 6ml, 16 ml, 1ml 13, 24, 45, 81 c-erbB-2 (HER-2/neu) EP3 AN726-5ME, AN726-10ME, AY726-YCDE, NU726UCE 6ml, 10ml, 16mL, 1mL 13, 24, 45, 81 Chromogranin A LK2H10 AM126-5M, AM126-10M, AX126-YCD, MU126-UC 6ml, 10ml, 16 ml, 1ml 13, 24, 45, 82, 186 Chromogranin A PHE-5 AM356-5M, AM356-10M, AX356-YCD, MU356-UC 6ml, 10ml, 16 ml, 1ml 13, 24, 45, 82, 187 Claudin 1 Polyclonal AR704-5R, AR704-10R, AW704-YCD, PU704-UP 6ml, 10 ml, 16 ml, 1ml 13, 24, 45, 82, 187 Claudin-5 EP224 AN718-5M, AN718-10M, AY718-YCD, NU718-UC 6 ml, 10 ml, 16mL, 1mL 13, 24, 45, 82, 187 c-myc Protein 9E10 AM318-5M, AX318-YCD, MU318-UC 6ml, 16 ml, 1ml 13. 45, 83, 187 Collagen III HWD1.1 AM167-5M, AX167-YCD, MU167-UC 6ml, 16 ml, 1ml 13, 48, 83, 187 Collagen IV COL-94 AM379-5M, AM379-10M, AX379-YCD, MU379-UC 6ml, 10ml, 16 ml, 1ml 14, 46, 83 Colloidal Iron stain - SS054-50X, SS054-YAK 50 Slides, 50 Slides 169, 173, 174 Common Ab Diluent - HK156-5KE 100 ml 176 Conc Goat Link - HK327-UG 5 ml 142 Super Sensitive Link Label IHC Detection System, HRP - LP000-ULE 1000 Slides 141 Conc Mouse Link - HK325-UM 5 ml 142 Conc Multilink - HK268-UK 5 ml 142 Conc Rabbit Link - HK326-UR 5 ml 142 Conc Strept Label AP - HK321-UK 5 ml 142 Conc Strept Label HRP - HK320-UK 5 ml 142 Congo Red Solution - SS028-50X, SS028-YAK 50 Slides, 50 Slides 168, 173, 174 Coverslips, 25X40mm, for Xmatrx - XT118-50X 50 each 20, 22 Coverslips, 25X40mm, for Xmatrx - XT118-YRK 500 each 20, 22 Coverslips, 18X18mm, for Xmatrx - XT121-YBX 175 each 20, 22 Coverslips, 25X25mm, for Xmatrx - XT122-90X 90 each 20, 22 Coverslips, 25X25mm, for Xmatrx - XT122-YQK 900 each 20, 22 Coverslips, 18X18mm, for Xmatrx - XT121-XBK 1750 each 20, 22 Cyclin D1 Polyclonal AR447-5R,AR447-10R, AW447-YCD, PU447-UP 6ml, 10 ml, 16 ml, 1ml 13, 24, 46, 83, 187 Cyclin D1 EPR2241(IHC)-32 AN474-5M, AN474-10M, AX474-YCD, NU474-UC 6 ml, 10 ml, 16 ml, 1 ml 13, 24, 46, 84, 187 Cyclin D1 Probe - PR216-100E, PR216-YADE 0.650 ml, 0.650 ml 19, 150, 151 13, 25, 46, 84, 187 Cyclin E2 EP454Y AN519-5M, AN519-10M, AX519-YCD, NU519-UC 6 ml, 10 ml, 16 ml, 1 ml Cytokeratin 4 6B10 AM705-5M, AM705-10M, AY705-YCD, MU705-UC 6 ml, 10 ml, 16ml, 1 ml 13, 25, 46, 84, 187 Cytokeratin 4 EP4 AN717-5M, AN717-10M, AY717-YCD, NU717-UC 6 ml, 10 ml, 16mL, 1mL 13, 25, 46, 84, 187 Cytokeratin 5 EPR1600Y AN484-5M, AN484-10M, AX484-YCD, NU484-UC 6 ml, 10 ml, 16 ml, 1 ml 13, 25, 46, 85 Cytokeratin 5 & 6 EPR1600Y + EPR1602Y AN588-5M, AN588-10M, AX588-YCD, NU588-UC 6 ml, 10 ml, 16 ml, 1 ml 13, 25, 46, 85, 187 198 www.biogenex.com Alphabetical Product Name Index Description Clone Cat. No Pack Size Page Cytokeratin 5 & Cytokeratin 14 EP24 + EP61 AN730-5ME, AN730-10ME, AY730-YCDE, NU730UCE 6ml , 10ml , 16ml, 1ml 13, 25, 46, 85 Cytokeratin 6 EPR1602Y AN500-5M, AN500-10M, AX500-YCD, NU500-UC 6 ml, 10 ml, 16 ml, 1 ml 13, 25, 46, 85, 187 Cytokeratin 7 EPR1619Y AN528-5M, AN528-10M, AX528-YCD, NU528-UC 6 ml, 10 ml, 16 ml, 1 ml 13, 25, 46, 86, 187 Cytokeratin 7 OV-TL12/30 AM255-5M, AM255-10M, AX255-YCD, MU255-UC 6ml, 10ml, 16 ml, 1ml 13, 25, 46, 86, 187 Cytokeratin 7&8 OV-TL12/30 & C51 AM587-5M, AM587-10M, AX587-YCD, MU587-UC 6 ml, 10 ml, 16 ml, 1 ml 14, 25, 46, 86, 187 Cytokeratin 8 C51 AM142-5M, AM142-10M, AX142-YCD, MU142-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 46, 86, 187 Cytokeratin 8 & 18 5D3 AM131-5M, AM131-10M, AX131-YCD, MU131-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 46, 87, 187 Cytokeratin 10 DEK-10 AM201-5M, AX201-YCD, MU201-UC 6ml, 16 ml, 1ml 14, 46, 87, 187 Cytokeratin 13 AE8 AM132-5M, AX132-YCD 6ml, 16 ml 14, 46, 87, 187 Cytokeratin 14 LL002 AM146-5M, AM146-10M, AX146-YCD, MU146-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 46, 87, 187 Cytokeratin 15 EPR1614Y AN485-5M, AN485-10M, AX485-YCD, NU485-UC 6 ml, 10 ml, 16 ml, 1 ml 14, 25, 46, 88 Cytokeratin 16 EP1615Y AN486-5M, AN486-10M, AX486-YCD, NU486-UC 6 ml, 10 ml, 16 ml, 1 ml 14, 25, 46, 88 Cytokeratin 17 E27 AM572-5M, AM572-10M, AX572-YCD, MU572-UC 6 ml, 10 ml, 16 ml, 1 ml 14, 25, 46, 88, 187 Cytokeratin 18 DC-10 AM143-5M, AX143-YCD, MU143-UC 6ml, 16 ml, 1ml 14, 46, 88, 187 Cytokeratin 19 RCK108 AM246-5M, AM246-10M, AX246-YCD, MU246-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 46, 89, 187 Cytokeratin 20 EPR1622Y AN557-5M, AN557-10M, AX557-YCD, NU557-UC 6 ml, 10 ml, 16 ml, 1 ml 14, 25, 46, 89, 187 Cytokeratin 20 IT-Ks20.8 AM315-5M, AM315-10M, MU315-UC, AX315-YCD 6ml, 10ml, 1ml 14, 25, 46, 89, 187 Cytokeratin Cocktail AE1 & AE3 AM071-5M, AM071-10M, AX071-YCD, MU071-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 46, 89, 187 Cytokeratin cocktail, Broad Spectrum 34βE12/C51/AE1 AM273-5M, AX273-YCD 6ml, 16 ml 14, 46, 90, 187 Cytokeratin cocktail, Broad Spectrum LL002+DEK10+RCK108+OVTL12/30+C11 AM372-5M, AM372-10M, AX372-YCD, MU372-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 46, 90, 187 Cytokeratin, High MW 34βE12 AM291-5M, AM291-10M, AX291-YCD 6ml, 10ml, 16 ml 14, 25, 46, 90, 187 Cytokeratin, High MW (Basic) AE3 AM133-5M, AX133-YCD, MU133-UC 6ml, 16 ml, 1ml 14, 46, 90, 187 Cytokeratin, Low MW AE1 AM075-5M, AM075-10M, AX075-YCD, MU075-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 46, 91, 187 Cytokeratin, Pan Lu-5 AM181-5M, AX181-YCD, MU181-UC 6ml, 16 ml, 1ml 14, 46, 91, 187 Cytokeratin, Pan C11 AM357-5M, AX357-YCD, MU357-UC 6ml, 16 ml, 1ml 14, 46, 91 Cytomegalovirus (CMV) BM204 AM254-5ME, MU254-UCE 6ml, 1ml 46, 91 Cytokeratin, High MW 34βE12 AM291-5M, AM291-10M, AX291-YCD 6ml, 10ml, 16 ml 14, 25, 46, 90, 187 DAB 2-Component Pack - HK542-XAK 1000 Slides 178 Desmin D33 AM072-5M, AM072-10M, AX072-YCD, MU072-UC 6 ml, 10 ml, 16 ml, 1 ml 14, 28, 46, 92,187 Diastase (a-Amylase) - EK004-5K 200 Slides 30, 38 14, 25, 46, 92, 187 D DOG1 1.1 AM570-5M, AM570-10M, AX570-YCD, MU570-UC 6 ml, 10 ml, 16 ml, 1 ml Dystrophin Dys1 (Dy4/6D3) AM243-5M, AX243-YCD 6ml, 16 ml 14, 46, 92, 187 Dystrophin Dys2 (Dy8/6C5) AM244-5M, AX244-YCD 6ml, 16 ml 14, 47, 92, 187 internationalcs@biogenex.com 199 Alphabetical Product Name Index Description Clone Cat. No Pack Size Page - PR205-100E, PR205-YADE 0.650 ml, 0.650 ml 19, 150, 151 AN725-5M, AN725-10M, AY725-YCD, NU725-UC 6 ml, 10 ml, 16mL, 1mL 14, 25, 47, 93, 187 E EBER Probe E-Cadherin EP6 E-Cadherin 36 AM390-5M, AM390-10M, AX390-YCD, MU390-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 47, 93 eFISH 19q13/19p13 Dual Color Probe - FP045-10XE, FP045-20XE 10 Tests, 20 Tests 162 eFISH 1p36/1q25 Dual Color Probe - FP044-10XE, FP044-20XE 10 Tests, 20 Tests 162 eFISH ALK Dual Color Break Apart Probe - FP056-10XE, FP056-20XE 10 Tests, 20 Tests 162 eFISH AML1/ETO Dual Color Dual Fusion Probe - FP072-10XE, FP072-20XE 10 Tests, 20 Tests 162 eFISH BCL2/IGH Dual Color Dual Fusion Probe - FP074-10XE, FP074-20XE 10 Tests, 20 Tests 162 eFISH BCR/ABL Dual Color Dual Fusion Probe - FP071-10XE , FP071-20XE 10 Tests, 20 Tests 162 eFISH BIRC3/MALT1 Dual Color Dual Fusion Probe - FP075-10XE, FP075-20XE 10 Tests, 20 Tests 162 eFISH CCND1 Dual Color Break Apart Probe - FP069-10XE , FP069-20XE 10 Tests, 20 Tests 162 eFISH CCND1/CEN 11 Dual Color Probe - FP063-10XE, FP063-20XE 10 Tests, 20 Tests 162 eFISH CCND1/IGH Dual Color Dual Fusion Probe - FP057-10XE, FP057-20XE 10 Tests, 20 Tests 162 eFISH CEN 17/HER2 Dual Color Probe - FP039-10XE, FP039-20XE 10 Tests, 20 Tests 162 eFISH CHOP Dual Color Break Apart Probe - FP050-10XE, FP050-20XE 10 Tests, 20 Tests 162 eFISH CMYC Dual Color Break Apart Probe - FP064-10XE, FP064-20XE 10 Tests, 20 Tests 162 eFISH CMYC/CEN 8 Dual Color Probe - FP065-10XE, FP065-20XE 10 Tests, 20 Tests 162 eFISH CMYC/IGH Dual Color Dual Fusion Probe - FP067-10XE, FP067-20XE 10 Tests, 20 Tests 162 eFISH COL1A1 Dual Color Break Apart Probe - FP054-10XE, FP054-20XE 10 Tests, 20 Tests 162 eFISH COL1A1/PDGFB Dual Color Dual Fusion Probe - FP052-10XE, FP052-20XE 10 Tests, 20 Tests 162 eFISH Cyto Kit - DF510-20XE 20 Tests 163 eFISH EGFR/CEN 7 Dual Color Probe - FP040-10XE, FP040-20XE 10 Tests, 20 Tests 162 eFISH EGR1/5p15 Dual Color Probe - FP068-10XE, FP068-20XE 10 Tests, 20 Tests 162 eFISH ETV6/RUNX1 Dual Color Dual Fusion Probe - FP076-10XE, FP076-20XE 10 Tests, 20 Tests 162 eFISH EWSR1 Dual Color Break Apart Probe - FP048-10XE, FP048-20XE 10 Tests, 20 Tests 162 eFISH FGFR1/CEN 8 Dual Color Probe - FP042-10XE, FP042-20XE 10 Tests, 20 Tests 162 eFISH FGFR2/CEN 10 Dual Color Probe - FP055-10XE, FP055-20XE 10 Tests, 20 Tests 162 eFISH FUS Dual Color Break Apart Probe - FP058-10XE, FP058-20XE 10 Tests, 20 Tests 162 eFISH Histo Kit - DF500-20XE 20 Tests 163 eFISH IGH Dual Color Break Apart Probe - FP070-10XE , FP070-20XE 10 Tests, 20 Tests 162 eFISH MDM2/CEN 12 Dual Color Probe - FP038-10XE, FP038-20XE 10 Tests, 20 Tests 162 eFISH MET/CEN 7 Dual Color Probe - FP047-10XE, FP047-20XE 10 Tests, 20 Tests 162 eFISH NMYC/2q11 Dual Color Probe - FP043-10XE, FP043-20XE 10 Tests, 20 Tests 162 eFISH p16/CEN 9 Dual Color Probe - FP041-10XE , FP041-20XE 10 Tests, 20 Tests 162 eFISH PDGFB Dual Color Break Apart Probe - FP053-10XE , FP053-20XE 10 Tests, 20 Tests 162 eFISH PIK3CA/CEN 3 Dual Color Probe - FP059-10XE, FP059-20XE 10 Tests, 20 Tests 162 eFISH PML/RARA Dual Color Dual Fusion Probe - FP073-10XE , FP073-20XE 10 Tests, 20 Tests 162 eFISH RET Dual Color Break Apart Probe - FP061-10XE, FP061-20XE 10 Tests, 20 Tests 162 eFISH ROS1 Dual Color Break Apart Probe - FP060-10XE, FP060-20XE 10 Tests, 20 Tests 162 eFISH SYT Dual Color Break Apart Probe - FP049-10XE, FP049-20XE 10 Tests, 20 Tests 162 eFISH TERT/5q31 Dual Color Probe - FP066-10XE, FP066-20XE 10 Tests, 20 Tests 162 eFISH TFE3 Dual Color Break Apart Probe - FP051-10XE , FP051-20XE 10 Tests, 20 Tests 162 eFISH TP53/CEN 17 Dual Color Probe - FP062-10XE, FP062-20XE 10 Tests, 20 Tests 162 eFISH U Kit - DF530-40KE 40 Tests 163 eFISH VHL/CEN 3 Dual Color Probe - FP046-10XE, FP046-20XE 10 Tests, 20 Tests 162 EGFR Polyclonal AR335-5RE, AW335-YCDE, PU335-UPE 6ml, 16ml, 1ml 14, 47, 93 EGFR(PAN) EP38Y AN473-5ME, AN473-10ME, AX473-YCDE, NU473UCE 6 ml, 10 ml, 16 ml, 1 ml 14, 25, 47, 93 200 www.biogenex.com Alphabetical Product Name Index Description Clone Cat. No Pack Size Page Elastic Stain - SS029-50X 50 Slides 169, 173 Elegance Red Substrate Pack - HK144-5KE 500 Slides 179 Empty Reagent Vials for Xmatrx Rx-Brown - XT078-V24 1each 21 Empty RFID Vial User Defined for ISH - XT079-PR0050 to XT079-PR0099 1each 20 Enhanced Antibody Diluent - HK941-YAKE 100ml 176 Ep-CAM E144 AN489-5M, AN489-10M, AX489-YCD, NU489-UC 6 ml, 10 ml, 16 ml, 1 ml 14, 25, 47, 94, 188 Epithelial Membrane Antigen (EMA) E29 AM057-5M, AM057-10M, AX057-YCD, MU057-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 47, 94, 187 Epithelial Membrane Antigen (EMA) Mc5 AM182-5M, AM182-10M, AX182-YCD, MU182-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 47, 94, 187 Epithelial-Specific Antigen MOC-31 AM316-5M, AM316-10M, MU316-UC, AX316-YCD 6ml, 10ml, 16 ml, 1ml 14, 25, 47, 94, 188 Epstein-Barr Virus Early Antigen 1108-1 AM222-5ME, MU222-UCE 6ml, 16ml, 1ml 47, 95, 188 ErbB-2/HER-2 EP1045Y AN471-5ME, AN471-10ME, AX471-YCDE 6 ml, 10 ml, 16 mL 13, 24, 47, 95 Estradiol Polyclonal AR038-5R, AR038-5RSG, AW038-YCD 6ml, 1ml, 16 ml 14, 47, 95, 188 Estrogen Receptor 1D5 AM272-2ME, AM272-10ME, AX272-YCDE, MU272UCE 3ml, 10ml, 16ml, 1ml 14, 25, 47, 95, 188 Estrogen Receptor (ER Alpha) EPR703(2) AN509-5ME, AN509-10ME, AX509-YCDE, NU509UCE 6 ml, 10 ml, 16 ml, 1 ml 14, 25, 47, 96 Estrogen Receptor (InSite® ER) ER88 AM368-5ME, AM368-10ME, AX368-YCDE, MU368UCE 6ml, 10ml, 16 ml, 1ml 14, 25, 47, 96, 188 Estrogen Receptor-Alpha EP1 AN710-5ME, AN710-10ME, AY710-YCDE, NU710UCE 6ml, 10 ml,16ml, 1ml 14, 25, 47, 96 Estrogen Receptor-Beta (ER-β) Polyclonal AR385-5R,AR385-10R, AW385-YCD, PU385-UP 6ml , 10ml , 16 ml, 1ml 14, 25, 47, 96 EZ-AR Common - HK545-XOK 1L 30, 35 EZ-AR1 Elegance, RTU - HK546-XAK 1L 34 EZ-AR1, RTU - HK521-XAK , HK521-XIK 1L, 2 Gallons 30, 35 EZ-AR2 Elegance, RTU - HK547-XAK 1L 34 EZ-AR2, RTU - HK522-XAK , HK522-XIK 1L, 2 Gallons 30, 35 EZ-AR3 - HK543-YOK 500 ml 30, 35 EZ-AR4 - HK544-YOK 500 ml 30, 35 EZ-AR™ 1 for Xmatrx - HX031-YCDE, HX031-YCX 200 test, 200 test 19 EZ-AR™ 2 for Xmatrx - HX032-YCDE, HX032-YCX 200 test, 200 test 19 EZ-AR™ 3 for Xmatrx - HX033-YCDE, HX033-YCX 200 test, 200 test 19 EZ-AR™ 4 for Xmatrx - HX034-YCDE, HX034-YCX 200 test, 200 test 19 EZ-DeWax Solution, Conc - HK584-5KE 500 ml 29, 32 Factor VIII-Related antigen F8 2.2.9 AM016-5M, AM016-10M, AX016-YCD, MU016-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 47, 97, 188 Factor XIII Subunit A E980.1 AM337-5M, AM337-10M, AX337-YCD, MU337-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 47, 97, 188 Factor XIII A EP3372 AN516-5M, AN516-10M, AX516-YCD, NU516-UC 6 ml, 10 ml, 16 ml, 1 ml 14, 25, 47, 97, 188 Fascin FCN01 AM488-5M, AM488-10M, AX488-YCD, MU488-UC 6 ml, 10 ml, 16 ml, 1 ml 14, 25, 47, 97, 188 Fast Red Substrate Pack - HK182-5KE 500 Slides 179 AN569-5M, AN569-10M, AX569-YCD, NU569-UC 6 ml, 10 ml, 16 ml, 1 ml 14, 25, 47, 98, 188 F Fibronectin F14 FISH Consumable Kit, for Xmatrx - XT147-YAD 100 slides 20, 22 Follicle Stimulating Hormone (FSH) Polyclonal AR026-5R, AW026-YCD 6ml, 16 ml 14, 47 Polyclonal AR019-5R, AW019-YCD, PU019-UP 6ml, 16 ml, 1ml 14, 47, 98, 188 14, 25, 47, 98, 188 169, 173, 174 G Gastrin GCDFP-15 EP1582Y AN481-5M, AN481-10M, AX481-YCD, NU481-UC 6 ml, 10 ml, 16 ml, 1 ml Geimsa Stain - SS049-50X, SS049-YAK 50 Slides, 50 Slides internationalcs@biogenex.com 201 Alphabetical Product Name Index Description Clone Cat. No Pack Size Page GLEPP-1 5C11 AM336-5M, AX336-YCD, MU336-UC 6ml, 16 ml, 1ml 14, 47, 99 Glial Fibrillary Acidic Protein (GFAP) GA-5 AM020-5M, AM020-10M, AX020-YCD, MU020-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 47, 99, 188 Glial Fibrillary Acidic Protein (GFAP) Polyclonal AR020-5R, AR020-10R, AW020-YCD, PU020-UP 6ml, 10ml, 16 ml, 1ml 14, 47, 99, 188 Glucagon Polyclonal AR039-5R, AW039-YCD, PU039-UP 6ml, 16 ml, 1ml 14, 47, 99, 188 14, 25, 47, 100, 188 Glut-1 SPM498 AM505-5M, AM505-10M, AX505-YCD, MU505-UC 6 ml, 10 ml, 16 ml, 1 ml Glutathione S-Transferase Pi (GST Pi) Polyclonal AR249-5R,AW249-YCD, PU249-UP 6ml, 16 ml, 1ml 14, 47, 100, 188 Glypican-3 (GPC3) GPC3-88 AM539-5M, AM539-10M, MU539-UC 6 ml, 10 ml, 1 ml 25, 47, 100, 188 GMS for Fungi Stain Kit - SS042-50X, SS042-YAK 50 Slides, 50 Slides 170, 173, 174 Goat, SS Neg Control - HK406-5G 3 ml 179 Gomoris Trichome Blue - SS033-50X, SS033-YAK 50 Slides, 50 Slides 169, 173, 174 Gomoris Trichome Greeen - SS034-50X, SS034-YAK 50 Slides, 50 Slides 170, 173, 174 Gram Stain Kit - SS037-50X, SS037-YAK 50 Slides, 50 Slides 170, 173, 174 Granulocyte BM-2 MU210-UC 1ml 47, 100, 188 H & E Retrieval Solution (10X) - HK169-5K 100 ml 37 Heat Shock Protein (HSP-70) BRM-22 AM289-5M, AX289-YCD, MU289-UC 6ml, 16 ml, 1ml 15, 47, 101, 188 Heat Shock Protein 27 (HSP 27) G3.1 AM171-5M, AM171-10M, AX171-YCD, MU171-UC 6ml, 10ml, 16 ml, 1ml 13, 26, 47, 101, 188 Helicobacter pylori Polyclonal AR442-5RE,AR442-10RE, AW442-YCDE, PU442UPE 6ml, 16ml, 1ml 15, 26, 47, 101 Hematoxylin - HK100-5K, HK100-9K 6ml, 250 ml 180 Hemoglobin A Polyclonal AR021-5R,AW021-YCD 6ml, 16 ml 15, 47, 102, 188 Hepatitis B Virus Core Antigen (HBcAg) Polyclonal AR082-5RE, PU082-UPE 6ml, 1ml 47, 102 Herpes Simplex Virus Type I (HSV I) Polyclonal AR084-5RE, AW084-YCDE, PU084-UPE 6ml, 16ml, 1ml 15, 47, 102 Herpes Simplex Virus Type II (HSV II) Polyclonal AR085-5RE, AW085-YCDE, PU085-UPE 6ml, 16ml, 1ml 15, 47, 102 H HGH Polyclonal AR707-5R, AR707-10R, AW707-YCD, PU707-UP 6ml, 10 ml, 16 ml, 1ml 15, 26, 47, 101, 188 HLA-DR LN3 AM154-5ME, AX154-YCDE, MU154-UCE 6ml, 16 ml, 1ml 15, 47, 103, 188 HPV 14 Probe - PR251-100E, PR251-YADE 0.650 ml, 0.650 ml 19, 150, 151 HPV 16/18 Probe - PR250-100E, PR250-YADE 0.650 ml, 0.650 ml 19, 150, 151 HSA HSA/E8 AM550-5M, AM550-10M, AX550-YCD, MU550-UC 6 ml, 10 ml, 16 ml, 1 ml 15, 25, 48, 103, 188 Hsa-miR-1 Probe - HM001-100E 0.650 ml 152, 157 Hsa-miR-106a Probe - HM106A-100E 0.650 ml 153, 157 Hsa-miR-10b Probe - HM010B-100E 0.650 ml 152, 157 Hsa-miR-125b Probe - HM125B-100E 0.650 ml 153, 157 Hsa-miR-126 Probe - HM126-100E 0.650 ml 153, 157 Hsa-miR-1285 Probe - HM1285-100E 0.650 ml 156, 157 Hsa-miR-141 Probe - HM141-100E 0.650 ml 153, 157 Hsa-miR-144 Probe - HM144-100E 0.650 ml 153, 157 Hsa-miR-146a Probe - HM146A-100E 0.650 ml 154, 157 Hsa-miR-147b Probe - HM147B-100E 0.650 ml 154, 157 Hsa-miR-150 Probe - HM150-100E 0.650 ml 154, 157 Hsa-miR-151a-3p Probe - HM151-100E 0.650 ml 154, 157 Hsa-miR-152 Probe - HM152-100E 0.650 ml 154, 157 Hsa-miR-155 Probe - HM155-100E 0.650 ml 154, 157 Hsa-miR-17 Probe - HM017-100E 0.650 ml 152, 157 Hsa-miR-196a Probe - HM196A-100E 0.650 ml 155, 157 Hsa-miR-200a Probe - HM200A-100E 0.650 ml 152, 157 Hsa-miR-200a Probe - HM200B-100E 0.650 ml 155, 157 Hsa-miR-204 Probe - HM204-100E 0.650 ml 155, 157 Hsa-miR-205 Probe - HM205-100E 0.650 ml 155, 157 202 www.biogenex.com Alphabetical Product Name Index Description Clone Cat. No Pack Size Page Hsa-miR-21 Probe - HM021-100E 0.650 ml 153, 157 Hsa-miR-216a Probe - HM216A-100E 0.650 ml 155, 157 Hsa-miR-222 Probe - HM222-100E 0.650 ml 155, 157 Hsa-miR-328 Probe - HM328-100E 0.650 ml 156, 157 Hsa-miR-375 Probe - HM375-100E 0.650 ml 156, 157 Hsa-miR-7a Probe - HM007A-100E 0.650 ml 156, 157 i500 S Bar Code Labeling System - BLS500 1 Unit 10, 30 i6000 Diagnostic - AS6030 1 Unit 8, 23 i6000 Infinity - AS6040 1 Unit 9, 23 i6000 Pipette Tips (192 tips) - XT105-01X 192 tips 20, 22, 29 i6000 Pipette Tips (960 tips) - XT104-05X 960 tips 20, 22, 29 IgA Polyclonal AR045-5R,AW045-YCD, PU045-UP 6ml, 16 ml, 1ml 15, 48, 103 IgD Polyclonal AR440-5R, AW440-YCD, PU440-UP 6ml, 16ml, 1ml 15, 48, 104, 188 IgG IgG88 AM367-5M, AX367-YCD, MU367-UC 6ml, 16 ml, 1ml 15, 48, 104, 188 IgG Polyclonal AR050-5R,AW050-YCD, PU050-UP 6ml, 16 ml, 1ml 15, 48, 104, 188 IgM IgM88 AM366-5M, AX366-YCD, MU366-UC 6ml, 16 ml, 1ml 15, 48, 104, 188 IgM Polyclonal AR427-5R, AR427-10R, AW427-YCD, PU427-UP 6ml, 10ml, 16 ml, 1ml 15, 48, 105, 188 Inhibin-Alpha R1 AM446-5M, AM446-10M, AX446-YCD, MU446-UC 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 105, 189 Insulin HB125 AM029-5M, AW029-YCD, AX029-YCD, MU029-UC 6ml, 16 ml, 16 ml, 1ml 15, 48, 105, 189 Insulin EP125 AN735-5M, AN735-10M, AY735-YCD, NU735-UC 6 ml, 10 ml, 16mL, 1mL 15, 26, 48, 106, 189 I Iron Stain Kit - SS030-50X, SS030-YAK 50 Slides, 50 Slides 170, 173, 174 ISH Consumable Kit, for Xmatrx - XT144-YAD 100 slides 20 J J Chain JC88 AM374-5M, AX374-YCD, MU374-UC 6ml, 16 ml, 1ml 15, 48, 106 Jones' Basement Membrane Stain - SS058-50X, SS058-YAK 50 Slides, 50 Slides 170, 173, 174 Kappa Light Chain L1C1 AM048-5M, AM048-10M, AX048-YCD, MU048-UC 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 106, 189 Kappa Light Chain K88 AM369-5M, AM369-10M, AX369-YCD, MU369-UC 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 106, 189 Kappa Probe - PR214-100E, PR214-YADE 0.650 ml, 0.650 ml 19, 151 14, 25, 48, 95, 188 K Ki-67 EP5 AN727-5M, AN727-10M, AY727-YCD, NU727-UC 6 ml, 10 ml, 16mL, 1mL Ki-67 K-2 AM410-5M, AM410-10M, AX410-YCD, MU410-UC 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 106, 189 Ki-67 EPR3611 AN527-5M, AN527-10M, AX527-YCD, NU527-UC 6 ml, 10 ml, 16 ml, 1 ml 15, 26, 48, 107, 189 Ki-67 and Lambda Light Chain Cocktail Mouse K-2 + Rabbit Poly AC562-5M, AC562-10M, AC562-YCD 6 ml, 10 ml, 16 ml 15, 26, 146, 189 Ki-67 Antigen, Proliferating Cell Ki88 AM370-5M, AM370-10M, AX370-YCD, MU370-UC 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 107, 189 Ki-67 Antigen, Proliferating Cell MIB-1 AM297-5M, AM297-10M, AX297-YCD, MU297-UC 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 107, 189 L Label Roll i500 PLUS - XT035-XBX 1each 30 Lambda Light Chain Polyclonal AR049-5R,AW049-YCD, PU049-UP 6ml, 16 ml, 1ml 15, 48, 108, 189 Lambda Light Chain EP172 AN715-5M, AN715-10M, AY715-YCD, NU715-UC 6 ml, 10 ml, 16mL, 1mL 15, 26, 48, 108, 189 Lambda Probe - PR215-100E, PR215-YADE 0.650 ml, 0.650 ml 19, 151 Laminin Polyclonal AR078-5R, AR078-10R, AW078-YCD, PU078-UP 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 108, 189 internationalcs@biogenex.com 203 Alphabetical Product Name Index Description Clone Cat. No Pack Size Page Link Diluent - HK165-5KE 100 ml 176 Liquid DAB Chromogen - HK124-7K, HK124-9K 4 ml, 10ml 178 Lysozyme Polyclonal AR024-5R,AR024-10R, AW024-YCD, PU024-UP 6ml,10ml, 16 ml, 1ml 15, 26, 48, 108, 189 15, 48, 109, 189 M Macrophage LN5 AM165-5M, AX165-YCD, MU165-UC 6ml, 16 ml, 1ml Masson's Trichrome Kit - SS035-50X, SS035-YAK 50 Slides, 50 Slides 170, 173, 174 Mast Cell Tryptase AA1 AM419-5M, AM419-10M, AX419-YCD, MU419-UC 6ml, 10ml, 16 ml, 1ml 26, 48, 109, 189 Mega Multilink/HRP Kit - QP300-XAK 1000 Slides 141 Melan-A (MART-1) A103 AM361-5M, AM361-10M, AX361-YCD, MU361-UC 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 109, 189 Melanoma HMB45 AM001-5M, AM001-10M, AX001-YCD, MU001A-UC 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 109, 189 Melanoma gp100 gp100/D5 AM536-5M, AM536-10M, AX536-YCD, MU536-UC 6 ml, 10 ml, 16 ml, 1 ml 15, 26, 48, 110, 189 Melanoma-Associated Antigen NKI/C3 AM077-5M, AM077-10M, AX077-YCD, MU077-UC 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 110, 189 Mesothelin 5B2 AM433-5M, AM433-10M, AX433-YCD, MU433-UC 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 110, 189 Microwave AR Accessory Kit - MW001-HB 3 units 34 Microwave AR Accessory Kit - MW001-SU 1 unit 34 15, 26, 48, 110, 189 MiTF MiTF/A13 AM554-5M, AM554-10M, AX554-YCD, MU554-UC 6 ml, 10 ml, 16 ml, 1 ml Mitochondrial Antigen 113-1 MU213-UC 1ml 48, 111 MLH1 ES05 AM703-5M, AM703-10M, AY703-YCD, MU703-UC 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 111, 189 MMP-9 EP1255Y AN504-5M, AN504-10M, AX504-YCD, NU504-UC 6 ml, 10 ml, 16 ml, 1 ml 15, 26, 48, 111, 189 MSH2 SP46 AN743-5M, AN743-10M, AY743-YCD, NU743-UC 6ml , 10ml , 16mL, 1mL 15, 26, 48, 111 MSH6 2D4B5 AM454-5M, AM454-10M, AX454-YCD, MU454-UC 6 ml, 10 ml, 16 ml, 1 ml 15, 26, 48, 112 ,189 MUC4 1G8 AM455-5M, AM455-10M, AX455-YCD, MU455-UC 6 ml, 10 ml, 16 ml, 1 ml 15, 26, 48, 112, 189 MUC5AC 45M1 AM456-5M, AM456-10M, AX456-YCD, MU456-UC 6 ml, 10 ml, 16 ml, 1 ml 15, 26, 48, 112, 189 Mucicarmine Stain - SS036-50X, SS036-YAK 50 Slides, 50 Slides 171, 173, 174 AN564-5M, AN564-10M, AX564-YCD, NU564-UC 6 ml, 10 ml, 16 ml, 1 ml 15, 26, 48, 112, 189 Mucin 1 (MUC1) EPR1023 Mucin 2 (MUC2) CCP58 AM358-5M, AX358-YCD, MU358-UC 6ml, 16 ml, 1ml 15, 48, 113 Multi-Drug Resistance Marker (P-Glycoprotein) MDR88 AM391-5M, AX391-YCD, MU391-UC 6ml, 16 ml, 1ml 15, 48, 113 Muscle Actins Actin 88 Cocktail AM381-5M, AX381-YCD, MU381-UC 6ml, 16 ml, 1ml 15, 48, 113 Myelin Basic Protein MBP88 AM380-5M, AM380-10M, AX380-YCD 6ml, 10ml, 16 ml 15, 26, 48, 113 Myeloid specific Antigen BM-3 AM216-5M, AX216-YCD 6ml, 16 ml 16, 48, 114 Myeloid specific Antigen BM-1 AM164-5M, MU164-UC, AX164-YCD 6ml, 16 ml, 1ml 16, 48, 114, 189 Myeloperoxidase (MPO) Polyclonal AR496-5R, AR496-10R, AW496-YCD, PU496-UP 6 ml, 10 ml, 16 ml, 1 ml 16, 26, 49, 114, 189 Myf4 LO26 AM432-5M, AM432-10M, AX432-YCD, MU432-UC 6ml, 10ml, 16 ml, 1ml 16, 26, 49, 114, 189 Myoglobin MG-1 AM012-5M, AX012-YCD, MU012-UC 6ml, 16 ml, 1ml 16, 49, 115, 189 Myoglobin Polyclonal AR012-5R, AW012-YCD, PU012-UP 6ml, 16 ml, 1ml 16, 49, 115, 189 Myosin Heavy Chains, Smooth Muscle SMMS.1 AM331-5M, AM331-10M, AX331-YCD, MU331-UC 6ml, 10ml, 16 ml, 1ml 16, 26, 49, 115 Myosin,Skeletal Muscle MY-32 AM109-5M, AX109-YCD, MU109-UC 6ml, 16 ml, 1ml 16, 49, 115, 189 IP64 AM701-5M, AM701-10M, AY701-YCD, MU701-UC 6ml, 10ml, 16 ml, 1ml 16, 26, 49, 116, 189 N Napsin A 204 www.biogenex.com Alphabetical Product Name Index Description Clone Cat. No Pack Size Page 16, 26, 49, 116, 189 N-Cadherin EPR1792Y AN565-5M, AN565-10M, AX565-YCD, NU565-UC 6 ml, 10 ml, 16 ml, 1 ml Neurofilament NE-14 AM073-5M, AM073-10M, AX073-YCD, MU073-UC 6ml, 10ml, 16 ml, 1ml 16, 26, 49, 116, 190 Neuron Specific Enolase (NSE) MIG-N3 AM055-5M, AM055-10M, AX055-YCD, MU055-UC 6ml, 10ml, 16 ml, 1ml 16, 26, 49, 116, 190 New Fuchsin Substrate Pack - HK183-5KE 400 Slides 179 16, 26, 49, 117, 190 16, 26, 49, 117, 190 NGF Receptor EP1039Y AN522-5M, AN522-10M, AX522-YCD, NU522-UC 6 ml, 10 ml, 16 ml, 1 ml NGF Receptor EP31 AN738-5M, AN738-10M, AY738-YCD, NU738-UC 6 ml, 10 ml, 16mL, 1mL Nordic Ware Pressure Cooker - NW001-PC 1 each 39 Nuclear Ribonucleoprotein 58-15 MU230-UC 1ml 49, 117, 190 Oct 2 EPR542 AN558-5M, AN558-10M, AX558-YCD, NU558-UC 6 ml, 10 ml, 16 ml, 1 ml 16, 26, 49, 117, 190 Oct 4 EP143 AN724-5M, AN724-10M, AY724-YCD, NU724-UC 6 ml, 10 ml, 16ml, 1ml 16, 28, 49, 118, 190 O Oil Red O Stain - SS043-50X, SS043-YAK 50 Slides, 50 Slides 169, 173, 174 Oligo d(T) Probe - PR217-100E, PR217-YADE 0.650 ml, 0.650 ml 19, 151 One-Step Polymer-HRP - QD620-YIKE 500 Slides 139 One-Step Polymer-HRP - QD630-XAKE 1000 Slides 139 Optimiser Reagent Vial (20mL) - XT026-601 to XT026-799, XT026-601P to XT026660P 1 each, 1 each 28 Optimiser Reagent Vial (20mL) - XT026-PR601 to XT026-PR615 1 each 28 Optimiser Reagent Vial (20mL) - XT026-V24 24/pk 20, 22, 28 Optimiser Reagent Vials, 20mL - XT101-24X 24 pack 28 Optimiser Vial Holders (24) - XT027-H24 24/pk 28 OptiPlus Barrier Slides, 2/3 Test Area - XT013-CL, XT013-SL 1440 Slides, 72 Slides 29, 181 OptiPlus Barrier Slides, 3 X 1/3 Test Area,20 Boxes - XT014-CL, XT014-SL 1440 Slides, 72 Slides 29, 181 OptiPlus Microscope Slides - XT002-CL, XT002-SL 1440 Slides, 72 Slides 181 Osteonectin OST1 AM387-5M, AX387-YCD, MU387-UC 6ml, 16 ml, 1ml 16, 49, 118 p105 Proliferation-Associated Nuclear Antigen 2B3 AM317-5M, AX317-YCD, MU317-UC 6ml, 16 ml, 1ml 16, 49, 118, p16 (INK4a) G175-405 AM540-5M, AM540-10M, MU540-UC 6 ml , 10 ml, 1 ml 26, 49, 118, 190 p16+Ki67 cocktail Mouse G175-405 + Rabbit EPR3611 AC601-5M, AC601-10M 6 ml, 10ml 26, 146, 190 p21/WAF1 4D10 AM434-5M, AX434-YCD, MU434-UC 6ml, 16 ml, 1ml 16, 49, 119, 190 AN492-5M, AN492-10M, AX492-YCD, NU492-UC 6 ml, 10 ml, 16 ml, 1 ml 16, 26, 49, 119, 190 P p27 (Kip1) Y236 p27 (Kip1) DCS72 AM396-5M, AX396-YCD, MU396-UC 6ml, 16 ml, 1ml 16, 49, 119 p34cdc2 (Cyclin Dependent Kinase) POH-1 AM301-5M, AX301-YCD, MU301-UC 6ml, 16 ml, 1ml 16, 49, 119, 190 P504S (AMACR) 13H4 AN449-5ME, AN449-10ME, AX449-YCDE, NU449UCE 6ml, 10ml, 16ml, 1ml 16, 26, 49, 120, 190 P504S (AMACR) RBT-AMACR AN538-5M, AN538-10M, AX538-YCD, NU538-UC 6 ml, 10 ml, 16 ml, 1 ml 16, 26, 49, 120, 190 p53 EP9 AN728-5M, AN728-10M, AY728-YCD, NU728-UC 6 ml, 10 ml, 16mL, 1mL 16, 26, 49, 120, 190 p53 Protein BP53-12-1 AM195-5M, AM195-10M, AX195-YCD, MU195-UC 6ml, 10ml, 16 ml, 1ml 16, 26, 49, 120, 190 p53 Protein DO7 AM239-5M, AM239-10M, AX239-YCD, MU239-UC 6ml, 10ml, 16 ml, 1ml 16, 27, 49, 121, 190 p53 Protein 1801 AM240-5M, AM240-10M, AX240-YCD, MU240-UC 6ml, 10ml, 16 ml, 1ml 16, 27, 49, 121, 190 p63 Protein 4A4 AM418-5M, AM418-10M, AX418-YCD, MU418-UC 6ml, 10ml, 16 ml, 1ml 16, 27, 49, 121, 190 internationalcs@biogenex.com 205 Alphabetical Product Name Index Description Clone Cat. No Pack Size Page p63 + CK5 Mouse 4A4 + Rabbit EPR1600Y AC602-5M, AC602-10M, AC602-YCD 6ml, 10ml, 16ml 16, 27, 147 PAP A40010 AM532-5M, AM532-10M, AX532-YCD, MU532-UC 6 ml, 10 ml, 16 ml, 1 ml 16, 27, 49, 121, 190 PAP Pen - XT001-PP 1 each 29, 181 Papillomavirus Type 16 (HPV-16) Cam Vir-1 AM362-5ME, AX362-YCDE, MU362-UCE 6ml, 16 ml, 1ml 16, 49, 121 PAS for Fungi Stain - SS053-50X, SS053-YAK 50 Slides, 50 Slides 171, 173, 174 PAS Staining Kit - SS032-50X, SS032-YAK 50 Slides, 50 Slides 171, 173, 174 PAX-5 ZP007 AM457-5M, AM457-10M, AX457-YCD, MU457-UC 6 ml, 10 ml, 16 ml, 1 ml 16, 27, 49, 122, 190 Paxillin (N-Term) Y113 AN521-5M, AN521-10M, AX521-YCD, NU521-UC 6 ml, 10 ml, 16 ml, 1 ml 16, 27, 49, 122, 190 PDCD4 EPR3432 AN524-5M, AN524-10M, AX524-YCD, NU524-UC 6 ml, 10 ml, 16 ml, 1 ml 16, 27, 49, 122, 190 PDGF Receptor-ß Y92 AN463-5M, AN463-10M, AX463-YCD, NU463-UC 6 ml, 10 ml, 16 ml, 1ml 16, 27, 49, 122, 190 Pepsin 4-Pack Kit - EK000-5K 150 Slides 38 Pepsin 4-Pack Kit - EK000-10K, EK000-10X 200 Slides, 200 Slides 19, 30 Perodic Acid Schiff (PAS) Diastase Stain - SS039-50X, SS039-YAK 50 Slides, 50 Slides 171, 173, 174 Peroxide Block - HK111-5K, HK111-50K 6ml, 50ml 177 PGP9.5 3D9 AM736-5ME, AM736-10ME, AY736-YCDE, MU736UCE 6ml , 10ml, 16 ml, 1ml 16, 27, 49, 123 Phosphate Buffer Saline (PBS) - HK091-5KE 120 ml 176 PIN4 (p63+CKHMW+P504S) Mouse 4A4+ Mouse34BE12+ Rabbit13H4 AM448-5ME, AM448-10ME, AX448-YCDE, MU448UCE 6ml, 10ml, 16ml, 1ml 16, 27, 147,190 Pipette Tips, 200ul (192 tips), for Xmatrx - XT146-01X 960 tips 21, 22 Pipette Tips, 200ul (960 tips)/5 Boxes, for Xmatrx - XT145-05X 960 tips 21, 22 Placental Alkaline Phosphatase (PLAP) PL8-F6 AM228-5M, AM228-10M, AX228-YCD, MU228-UC 6ml, 10ml, 16 ml, 1ml 16, 27, 49, 123, 190 Placental Lactogen (hPL) Polyclonal AR040-5R,AR040-10R, AW040-YCD, PU040-UP 6ml, 10ml, 16 ml, 1ml 16, 27, 49, 123, 190 Platelet-Derived Growth Factor (PDGF) PDGF88 AM376-5M, AX376-YCD, MU376-UC 6ml, 16 ml, 1ml 16, 49, 123 Platelet-Derived Growth Factor (PDGF) Polyclonal AR376-5R, AW376-YCD, PU376-UP 6ml,16 ml, 1ml 16, 49, 124 PMS2 EPR3947 AN577-5M, AN577-10M, AX577-YCD, NU577-UC 6 ml, 10 ml, 16 ml, 1 ml 16, 27, 49, 124, 190 PMSA EP192 AN714-5M, AN714-10M, AY714-YCD, NU714-UC 6 ml, 10 ml, 16mL, 1mL 17, 27, 50, 126, 190 Positive Control on Barrier Slides - FB-001M to FB-743N 5/PK 184 - 191 Positive Control Slides - FG-001M to FG-743N 5/PK 184 - 191 Power Block (10X) - HK085-5KE 100 ml 177 Power Block Reagent, Ready-to-use - BS-1310-25 25ml 177 Progesterone Receptor EP2 AN711-5ME, AN711-10ME, AY711-YCDE, NU711UCE 6ml, 10ml, 16 ml, 1ml 16, 27, 49, 124 Progesterone Receptor 1A6 AM172-2ME, AM172-10ME, AX172-YCDE, MU172UCE 3ml, 10ml, 16ml, 1ml 16, 27, 49, 124 Progesterone Receptor (InSite®PR) PR88 AM328-5ME, AM328-10ME, AX328-YCDE, MU328UCE 6ml, 10ml, 16 ml, 1ml 16, 27, 49, 125, 190 Prolactin ME.121 AM031-5M, AM031-10M, AX031-YCD, MU031-UC 6ml, 10ml, 16 ml, 1ml 16, 28, 49, 125 Proliferating Cell Nuclear Antigen (PCNA) PC10 AM252-5M, AM252-10M, AX252-YCD, MU252-UC 6ml, 10ml, 16 ml, 1ml 16, 27, 49, 125, 190 Prostate Specific Acid Phosphatase (PSAP) B01-94-21M-NA AM013-5ME, AM013-10ME, AX013-YCDE, MU013UCE 6ml, 10ml, 16 ml, 1ml 16, 27, 50, 125, 190 Prostate Specific Antigen(PSA) ErPr8 AM014-5ME, AM014-10ME, AX014-YCDE, MU014UCE 6ml , 10ml , 16 ml, 1ml 16, 27, 50, 126, 190 Protease XXIV 4-Pack Kit - EK002-5K 150 Slides 38 Protease XXIV 4-Pack Kit - EK002-10K, EK002-10X 200 Slides, 200 Slides 19, 30 6ml, 10ml, 16 ml, 1ml 17, 27, 50, 126, 190 pS2 Estrogen Inducible Protein 206 PS2.1 AM190-5M, AM190-10M, AX190-YCD, MU190-UC www.biogenex.com Alphabetical Product Name Index Description Clone Cat. No Pack Size Page R Rabbit, SS Neg Control - HK408-5R, HK408-7R 3 ml, 17 ml 179 Rat, SS Neg Control - HK407-5T 3 ml 179 RCC (Renal Cell Carcinoma) RCC-26 AM543-5M, AM543-10M, AX543-YCD, MU543-UC 6 ml, 10 ml, 16 ml, 1 ml 17, 27, 50, 126, 190 21, 22 Reagent Vial Insert for RFID - XT149-V24 24/PK Renal Masson's Trichome - SS050-50X, SS050-YAK 50 Slides, 50 Slides 171, 173, 174 Resin Ribbon i500 PLUS - XT034-XEX 1each 30 Reticulin/No Counterstain Stain Kit - SS046-50X, SS046-YAK 50 Slides, 50 Slides 172, 173, 174 Reticulin/No Counterstain Stain Kit - SS047-50X, SS047-YAK 50 Slides, 50 Slides 172, 173, 174 Retinoblastoma (RB) Probe - PR225-100E, PR225-YADE 0.650 ml, 0.650 ml 19, 151 S100 Beta EP32 AN713-5M, AN713-10M, AY713-YCD, NU713-UC 6 ml, 10 ml, 16mL, 1mL 17, 27, 50, 127, 190 S-100 Protein 15E2E2 AM058-5M, AM058-10M, AX058-YCD, MU058-UC 6ml, 10ml, 16ml, 1ml 17, 27, 50, 127, 190 S-100 Protein Polyclonal AR058-5R, AR058-10R, AW058-YCD, PU058-UP 6ml, 10ml, 16 ml, 16 ml, 1ml 17, 27, 50, 127, 190 S100P EP186 AN712-5M, AN712-10M, AY712-YCD, NU712-UC 6 ml, 10 ml, 16mL, 1mL 17, 27, 50, 127, 190 S S-A HRP Diluent - HK157-5KE 100 ml 176 Safranin O Staining Protocol - SS040-50X, SS040-YAK 50 Slides, 50 Slides 172, 173, 174 Sarcomeric Actin ZMSA-5 AM511-5M, AM511-10M, AX511-YCD, MU511-UC 6 ml , 1 ml, 16 ml, 1 ml 17, 27, 50, 128, 190 Scramble Probe - PR032-100E 0.650 ml 156, 157 Secretin Polyclonal AR067-5R, AR067-5RSG, AW067-YCD 6ml, 1ml, 16 ml 17, 50, 128, 191 Slide Barcode Labels - AM6010 TO AM7990 100/SHEET 29 SS Double Stain Polymer Detection Kit I/DAB & Fast Red - QS200-60K 60 Slides 144 SS Double Stain Polymer Detection Kit I/DAB & Fast Red - QS200-YADE 100 slides 18 SS Double Stain Polymer Detection Kit I/Large Volume - QS210-YIK 500 Slides 144 SS Double Stain Polymer Detection Kit II/DAB & Fast Red - QS400-60K 60 Slides 144 SS Double Stain Polymer Detection Kit II/DAB & Fast Red - QS400-YADE 100 slides 18 SS Double Stain Polymer Detection Kit II/Large Volume - QS410-YIK 500 Slides 144 SS Goat Link - HK337-5G 6ml 142 SS Mouse Link - HK335-5M, HK335-9M 6ml, 50 ml 142 SS Mouse Negative Control - HK119-7M 17 ml 179 SS Multilink - HK340-5K, HK340-9K 6ml, 50 ml 142 SS Multilink Kit,HRP/DAB - QD470-60K, QD470-YCX 60 Slides, 200 Slides 28, 141 SS One Step Polymer-HRP IHC Detection System for Xmatrx - QD610-YAXE 200 slides 28 SS One Step Polymer-HRP IHC Detection System for Xmatrx - QD610-YADE 200 Slides 18 SS Rabbit Link - HK336-5R, HK336-9R 6ml, 50 ml 142 SS Rat Link - HK338-5T 6ml 142 SS Strept Alka Phos Label - HK331-5K, HK331-9K 6ml, 50 ml 142 17, 27, 50, 128,191 17, 50, 128, 191 AR6010 TO AR6600 Stat 5-Alpha (C-term) E289 AN480-5M, AN480-10M, AX480-YCD, NU480-UC 6 ml , 10 ml, 16 ml, 1 ml Substance P Polyclonal AR069-5R, AW069-YCD, PU069-UP 6ml,16 ml, 1ml Sudan Black B Stain - SS041-50X, SS041-YAK 50 Slides, 50 Slides 172, 173, 174 Super Mount - HK079-5K, HK079-7K 15 ml, 50 ml 180 internationalcs@biogenex.com 207 Alphabetical Product Name Index Description Clone Cat. No Pack Size Page Super Sensitive HRP Label - HK330-5K, HK330-9K 6ml, 50 ml 142 Super Sensitive Link HRP IHC Detection System - QP000-5L 50-60 Slides 141 Super Sensitive Link Label IHC Detection System, Alk Phos - QA900-9L 500 Slides 141 Super Sensitive Link Label IHC Detection System, AP - LA000-ULE 1000 Slides 141 Super Sensitive Link Label IHC Detection System, HRP/DAB - QD420-YIKE 500 Slides 139 Super Sensitive Link Label IHC Detection System, HRP/DAB Mega - QD430-XAKE 1000 Slides 139 Super Sensitive Non-Biotin HRP, DAB Automated Kit - QD410-YAXE 200 Slides 28 Super Sensitive Non-Biotin HRP, DAB Kit - QD400-60KE 60 Slides 139 Super Sensitive Link HRP IHC Detection System - QP900-9L 500 Slides 141 Super Sensitive One Step Polymer-HRP ISH Detection System - DF400-50KE 50 Slides 158 Super Sensitive One Step Polymer-HRP ISH Detection System - DF400-YADE 100 slides 19, 158 Super Sensitive Polymer-HRP IHC Kit - QD440-XAK 1000 Slides 139 Super Sensitive Wash Buffer, 20X - HK583-5KE 500 ml 30, 176 17, 27, 50, 129, 191 Survivin EP2880Y AN503-5M, AN503-10M, AX503-YCD, NU503-UC 6 ml, 10 ml, 16 ml, 1 ml Synaptophysin Snp88 AM363-5M, AM363-10M, AX363-YCD, MU363-UC 6ml, 10ml, 16 ml, 1ml 17, 27, 50, 129, 191 Tau TAU-2 AM412-5M, AM412-10M, AX412-YCD, MU412-UC 6ml, 10ml, 16 ml, 1ml 17, 27, 50, 129, 191 Tau Tau-5 AM459-5M, AM459-10M, AX459-YCD, MU459-UC 6 ml, 10 ml, 16 ml, 1 ml 17, 27, 50, 129, 191 Terminal Deoxynucleotidyl Transferase (TdT) TdT88 AM373-5M, AM373-10M, AX373-YCD, MU373-UC 6ml, 10ml, 16 ml, 1ml 13, 27, 50, 130 Terminal Deoxynucleotidyl Transferase (TdT) Polyclonal AR373-5R, AW373-YCD 6ml, 16 ml 17, 50, 130, 191 TFPU.1 EPR3159Y AN520-5M, AN520-10M, AX520-YCD, NU520-UC 6 ml, 10 ml, 16 ml, 1 ml 17, 27, 52, 130, 191 Thyroglobulin 2H11 AM032-5M, AM032-10M, AX032-YCD, MU032-UC 6ml, 10ml, 16 ml, 1ml 17, 27, 50, 130, 191 Thyroid Stimulating Hormone (TSH) 5404 AM033-5M, AM033-10M, AX033-YCD, MU033-UC 6ml, 10ml, 16 ml, 1ml 17, 27, 50, 131 Thyroid Stimulating Hormone (TSH) Polyclonal AR033-5R, AW033-YCD 6ml, 16 ml 17, 50, 131 Thyroxine D5 AM034A-5M, AX034-YCD, MU034A-UC 6ml, 16 ml, 1ml 17, 50, 131, 191 TIA -1 2G9A10F5 AM529-5M, AM529-10M, AX529-YCD, MU529-UC 6 ml, 10 ml, 16 ml, 1 ml 17, 27, 50, 132 Toluidine blue stain - SS057-50X, SS057-YAK 50 Slides, 50 Slides 172, 173, 174 17, 27, 50, 132, 191 50, 132 T Topo 2 Alpha EP1102Y AN576-5M, AN576-10M, AX576-YCD, NU576-UC 6 ml, 10 ml, 16 ml, 1 ml Toxoplasma gondii Polyclonal AR125-5RE, PU125-UPE 6ml, 1ml Transferrin HT1/13.6.3 AM025-5M, AX025-YCD 6ml, 16 ml 17, 50, 132, 191 Transforming Growth Factor (TGF),Alpha TGF88 AM377-5M, AX377-YCD, MU377-UC 6ml, 16 ml, 1ml 17, 50, 133 Tris Buffer, Conc pH 7.6 - HK098-5K 3/PK 176 Trypsin 4-Pack Kit - EK001-5K 150 Slides 38 Trypsin 4-Pack Kit - EK001-10K, EK001-10X 200 Slides, 200 Slides 19, 30 TTF-1 8G7G3/1 AM397-5M, AM397-10M, AX397-YCD, MU397-UC 6ml, 10ml, 16 ml, 1ml 17, 27, 50, 131, 191 TTF1 and Cytokeratin 5/6 cocktail Mouse 8G7G3/1 + Rabbit EPR1600Y + Rabbit1602Y AC563-5M, AC563-10M, AC563-YCD 6 ml, 10 ml, 16 ml 17, 27, 146, 191 TTF-1+CK20+CK7 cocktail Mouse 8G7G3/1 + Rabbit EP1582Y + EP1619Y AC605-5M, AC605-10M, AC605-YCD 6 ml, 16 ml 17, 27, 147 TTF-1+GCDFP-15 cocktail Mouse 8G7G3/1 + Rabbit EP1582Y AC604-5M, AC604-10M, AC604-YCD 6 ml, 16 ml 17, 27, 146, 191 208 www.biogenex.com Alphabetical Product Name Index Description Clone Cat. No Pack Size Page 6ml, 10ml, 16 ml, 1ml 17, 27, 50, 133, 191 Tumor-Associated Glycoprotein (TAG-72) B72.3 AM054-5M, AM054-10M, AX054-YCD, MU054-UC Tumor-Associated Glycoprotein (TAG-90 BCA) B6.2 AM005-5M, AX005-YCD, MU005-UC 6ml, 16 ml, 1ml 17, 50, 133, 191 Ty/G5 AM535-5M, AM535-10M, AX535-YCD, MU535-UC 6 ml, 10 ml, 16 ml, 1 ml 17, 27, 50, 133, 191 Tyrosinase U U6 Probe - PR031-100E 0.650 ml 156, 157 User Defined Empty RFID Tagged Vials Two Step IHC - XT077-V24 1each 21 User Defined Empty RFID Tagged Vials Two Step IHC - XT077-AX0801 to XT0821-AX0999 1 each 20 User Defined Empty RFID Tagged Vials Two Step IHC - XT077-AX0601 to XT077-AX0800 1 each 20 - SS044-50X, SS044-YAK 50 Slides, 50 Slides 172, 173, 174 17, 27, 50, 134, 191 V Van Gieson Stain VEGF Polyclonal AR483-5R, AR483-10R, AW483-YCD, PU483-UP 6 ml, 10 ml, 16 ml, 1 ml Villin ID2C3 AM525-5M, AM525-10M, AX525-YCD, MU525-UC 6 ml, 10 ml, 16 ml, 1 ml 17, 27, 50, 134, 191 Vimentin V9 AM074-5M, AM074-10M, AX074-YCD, MU074-UC 6ml, 10ml, 16 ml, 1ml 17, 27, 50, 134, 191 Vimentin,Non-Hematopoietic LN6 AM163-5M, AX163-YCD, MU163-UC 6ml, 16 ml, 1ml 17, 50, 134, 191 17, 27, 50, 135, 191 VIP Polyclonal AR530-5R, AR530-10R, AW530-YCD, PU530-UP 6 ml, 10 ml, 16 ml, 1 ml von Kossa stain - SS045-50K 50 Slides 173, 174 CAN-R9(IHC)-56-2 AN584-5M, AN584-10M, AX584-YCD, NU584-UC 6 ml, 10 ml, 16 ml, 1 ml 17, 27, 50, 135, 191 W Wilm's Tumor X X-DeWax - HX016-XAK, HX016-XEK 1000 ml, 1 Gallon 21 Xflex Consumable Kit, for Xmatrx - XT132-YCD 200 slides 20 Xflex Ultra Consumable Kit, for Xmatrx - XT148-YCD 200 slides 20 Xmatrx Elite - AS4040B 1 Unit 2, 7, 11 Xmatrx Infinity - AS4000RX 1 Unit 6, 11 Xmatrx Mini - AS1010 1 Unit 5, 7, 11 Xmatrx Nano - AS1000 1 Unit 4, 7, 11 Xmatrx Reagent Vials - XT126-24V 24-/PK 20, 22 AS4030B 1 Unit 3, 11 Xmatrx Ultra Xmount - HX035-04X, HX035-10X, HX035-40D 4 ml, 10 ml, 10 ml 21 XVIZ Detection Kit - QD550-YCDE, QD550-YCX 200 slides, 200 slides 18 X-Wash Buffer, 20X, for Xmatrx - HX020-YIK 500 ml 21 X-Wash ISH Wash Buffer - HX017-YIK 500 ml 21 ZAP-70 YE291 AN466-5M, AN466-10M, AX466-YCD, NU466-UC 6 ml, 10 ml, 16 ml, 1ml 17, 27, 50, 135, 191 ZAP-70 ZAP70-C3 AM544-5M, AM544-10M, AX544-YCD, MU544-UC 6 ml, 10 ml, 16 ml, 1 ml 17, 27, 50, 135, 191 Z internationalcs@biogenex.com 209 Catalog Number Index Cat. No. Product Name Page A Cat. No. Product Name Page AM059-5ME, AX059YCDE, MU059-UCE Adenovirus 11, 43, 52 AC559-5M, AC559-10M, AC559-YCD CD3 and CD20 cocktail 12, 23, 145, 185 AM071-5M, AM071-10M, AX071-YCD, MU071-UC Cytokeratin Cocktail 14, 25, 46, 89, 187 AC561-5M, AC561-10M, AC561-YCD CD10 and Cyclin D1 cocktail 12, 24, 145, 185 AM072-5M, AM072-10M, AX072-YCD, MU072-UC Desmin 14, 28, 46, 92,187 AC562-5M, AC562-10M, AC562-YCD Ki-67 and Lambda Light Chain Cocktail 15, 26, 146, 189 AM073-5M, AM073-10M, AX073-YCD, MU073-UC Neurofilament 16, 26, 49, 116, 190 AC563-5M, AC563-10M, AC563-YCD TTF1 and Cytokeratin 5/6 cocktail 17, 27, 146, 191 AM074-5M, AM074-10M, AX074-YCD, MU074-UC Vimentin 17, 27, 50, 134, 191 AC595-5M, AC59510M,AC595-YCD CD4 + C8 Cocktail 12, 23, 145 AM075-5M, AM075-10M, AX075-YCD, MU075-UC Cytokeratin, Low MW 14, 25, 46, 91, 187 AC596-5M, AC596-10M, AC596-YCD CDX-2+CK7 cocktail 13, 24, 145, 186 AM077-5M, AM077-10M, AX077-YCD, MU077-UC Melanoma-Associated Antigen 15, 26, 48, 110, 189 AC597-5M, AC597-10M, AC597-YCD CDX-2+CK20+CK7 cocktail 13, 24, 147 AM090-5M, AM090-10M, AX090-YCD, MU090-UC Actin, Muscle-Specific 11, 23, 43, 51, 184 AC601-5M, AC601-10M p16+Ki67 cocktail 26, 146, 190 AM097-5M, AX097-YCD, MU097-UC Alpha-Actinin 11, 43, 52, 184 AC602-5M, AC602-10M, AC602-YCD Mouse 4A4 + Rabbit EPR1600Y 16, 27, 147 AM109-5M, AX109-YCD, MU109-UC Myosin,Skeletal Muscle 16, 49, 115, 189 AC604-5M,AC604-10M, AC604-YCD TTF-1+GCDFP-15 cocktail 17, 27, 146, 191 AM111-5M, AM111-10M, AX111-YCD CD45 AC605-5M, AC605-10M, AC605-YCD 12, 24, 45, 72, 186 TTF-1+CK20+CK7 cocktail 17, 27, 147 13, 24, 45, 74, 186 Melanoma 15, 26, 48, 109, 189 AM113-5M, AM113-10M, AX113-YCD, MU113-UC CD45RO (T Cell) AM001-5M, AM001-10M, AX001-YCD, MU001A-UC Tumor-Associated Glycoprotein (TAG-90 BCA) AM126-5M, AM126-10M, AX126-YCD, MU126-UC Chromogranin A AM005-5M, AX005-YCD, MU005-UC 13, 24, 45, 82, 186 17, 50, 133, 191 AM128-5M, AM128-10M, AX128-YCD, MU128-UC Actin, Smooth Muscle 11, 23, 43, 51, 184 Alpha-Fetoprotein (AFP) 11, 23, 43, 53, 184 AM131-5M, AM131-10M, AX131-YCD, MU131-UC Cytokeratin 8 & 18 14, 25, 46, 87, 187 Carcinoembryonic Antigen (CEA) 11, 23, 43, 60, 185 AM132-5M, AX132-YCD Cytokeratin 13 14, 46, 87, 187 Cytokeratin, High MW (Basic) 14, 46, 90, 187 AM008-5M, AM008-10M, AX008-YCD, MU008A-UC AM009-5M, AM009-10M, AX009-YCD, MU009-UC AM012-5M, AX012-YCD, MU012-UC Myoglobin 16, 49, 115, 189 AM133-5M, AX133-YCD, MU133-UC AM013-5ME, AM01310ME, AX013-YCDE, MU013-UCE Prostate Specific Acid Phosphatase (PSAP) 16, 27, 50, 125, 190 AM134-5ME, AM13410ME, AX134-YCDE, MU134-UCE c-erbB-2 (HER-2/neu) 13, 24, 45, 81 AM014-5ME, AM01410ME, AX014-YCDE, MU014-UCE Prostate Specific Antigen(PSA) 16, 27, 50, 126, 190 AM135-5M, AM135-10M, AX135-YCD Breast Cancer Antigen BCA 225 11, 23, 43, 58, 184 AM016-5M, AM016-10M, AX016-YCD, MU016-UC 14, 25, 47, 97, 188 AM142-5M, AM142-10M, AX142-YCD, MU142-UC Cytokeratin 8 Factor VIII-Related antigen 14, 25, 46, 86, 187 AM020-5M, AM020-10M, AX020-YCD, MU020-UC Glial Fibrillary Acidic Protein (GFAP) 14, 25, 47, 99, 188 AM143-5M, AX143-YCD, MU143-UC Cytokeratin 18 14, 46, 88, 187 AM025-5M, AX025-YCD Transferrin 17, 50, 132, 191 AM146-5M, AM146-10M, AX146-YCD, MU146-UC Cytokeratin 14 14, 25, 46, 87, 187 AM029-5M, AW029-YCD, AX029-YCD, MU029-UC Insulin 15, 48, 105, 189 AM152-5M, AX152-YCD, MU152-UC CDw75 (B Cell) 13, 45, 80, 186 AM031-5M, AM031-10M, AX031-YCD, MU031-UC Prolactin 16, 28, 49, 125 AM153-5M, AX153-YCD, MU153-UC CD74 (B Cell) 13, 45, 77, 186 AM032-5M, AM032-10M, AX032-YCD, MU032-UC Thyroglobulin 17, 27, 50, 130, 191 AM154-5ME, AX154YCDE, MU154-UCE HLA-DR 15, 47, 103, 188 AM033-5M, AM033-10M, AX033-YCD, MU033-UC Thyroid Stimulating Hormone (TSH) 17, 27, 50, 131 AM156-5M, AX156-YCD, MU156-UC CD45RC (T Cell) 12, 45, 74, 186 AM034A-5M, AX034-YCD, MU034A-UC Thyroxine 17, 50, 131, 191 AM157-5M, AM157-10M, AX157-YCD CD45RA (B Cell) 12, 24, 45, 73, 186 AM048-5M, AM048-10M, AX048-YCD, MU048-UC Kappa Light Chain 15, 26, 48, 106, 189 AM158-5M, AM158-10M, AX158-YCD, MU158-UC B Cell 11, 43, 54, 184 AM054-5M, AM054-10M, AX054-YCD, MU054-UC Tumor-Associated Glycoprotein (TAG-72) 17, 27, 50, 133, 191 AM159-5M, AX159-YCD CD43 & CD45RA Cocktail 12, 44, 72, 186 AM055-5M, AM055-10M, AX055-YCD, MU055-UC 16, 26, 49, 116, 190 Vimentin,Non-Hematopoietic 17, 50, 134, 191 Neuron Specific Enolase (NSE) AM163-5M, AX163-YCD, MU163-UC AM057-5M, AM057-10M, AX057-YCD, MU057-UC Epithelial Membrane Antigen (EMA) 14, 25, 47, 94, 187 AM164-5M, MU164-UC, AX164-YCD Myeloid specific Antigen 16, 48, 114, 189 AM058-5M, AM058-10M, AX058-YCD, MU058-UC 17, 27, 50, 127, 190 AM165-5M, AX165-YCD, MU165-UC Macrophage 15, 48, 109, 189 S-100 Protein 210 www.biogenex.com Catalog Number Index Cat. No. Product Name Page Cat. No. Product Name Page AM167-5M, AX167-YCD, MU167-UC Collagen III 13, 48, 83, 187 AM273-5M, AX273-YCD Cytokeratin cocktail, Broad Spectrum 14, 46, 90, 187 AM171-5M, AM171-10M, AX171-YCD, MU171-UC Heat Shock Protein 27 (HSP 27) 13, 26, 47, 101, 188 AM287-5M, AM287-10M, AX287-YCD Bcl-2 Oncoprotein 11, 23, 43, 55, 184 AM172-2ME, AM17210ME, AX172-YCDE, MU172-UCE Progesterone Receptor 16, 27, 49, 124 AM289-5M, AX289-YCD, MU289-UC Heat Shock Protein (HSP-70) 15, 47, 101, 188 AM181-5M, AX181-YCD, MU181-UC 14, 46, 91, 187 AM291-5M, AM291-10M, AX291-YCD Cytokeratin, High MW Cytokeratin, Pan 14, 25, 46, 90, 187 AM182-5M, AM182-10M, AX182-YCD, MU182-UC Epithelial Membrane Antigen (EMA) 14, 25, 47, 94, 187 AM297-5M, AM297-10M, AX297-YCD, MU297-UC Ki-67 Antigen, Proliferating Cell 15, 26, 48, 107, 189 AM190-5M, AM190-10M, AX190-YCD, MU190-UC 17, 27, 50, 126, 190 AM298-5M, AX298-YCD, MU298-UC CD29 12, 44, 69, 185 pS2 Estrogen Inducible Protein AM195-5M, AM195-10M, AX195-YCD, MU195-UC 16, 26, 49, 120, 190 AM301-5M, AX301-YCD, MU301-UC p34cdc2 (Cyclin Dependent Kinase) 16, 49, 119, 190 p53 Protein AM201-5M, AX201-YCD, MU201-UC 14, 46, 87, 187 AM302-5M, AM302-10M, AX302-YCD, MU302-UC CD15 Cytokeratin 10 12, 24, 44, 67, 185 AM216-5M, AX216-YCD Myeloid specific Antigen 16, 48, 114 AM305-5M, AX305-YCD, MU305-UC CD43 (T Cell, Leukosialin) 12, 44, 72, 186 AM222-5ME, MU222UCE Epstein-Barr Virus Early Antigen 47, 95, 188 AM310-5M, AX310-YCD, MU310-UC CD44 (Phagocytic Glycoprotein1,HCAM) 12, 45, 72, 186 AM228-5M, AM228-10M, AX228-YCD, MU228-UC Placental Alkaline Phosphatase (PLAP) 16, 27, 49, 123, 190 AM314-5M, AX314-YCD, MU314-UC CD57 (Natural Killer Cell) 13, 45, 75, 186 AM231-5M, AM231-10M, AX231-YCD, MU231-UC B Lymphocyte Antigen 36 (BLA.36) 11, 23, 43, 54, 184 AM315-5M, AM315-10M, MU315-UC, AX315-YCD Cytokeratin 20 14, 25, 46, 89, 187 AM232-5M, AM232-10M, AX232-YCD CD31 12, 24, 44, 70, 185 AM316-5M, AM316-10M, MU316-UC, AX316-YCD Epithelial-Specific Antigen 14, 25, 47, 94, 188 AM236-5M, AM236-10M, AX236-YCD, MU236-UC CD34 (Endothelial Cell) 12, 24, 44, 71, 185 AM317-5M, AX317-YCD, MU317-UC p105 Proliferation-Associated Nuclear Antigen 16, 49, 118, AM238-5M, AM238-10M, AX238-YCD, MU238A-UC CD20 (B Cell) 12, 24, 44, 185 AM318-5M, AX318-YCD, MU318-UC c-myc Protein 13. 45, 83, 187 AM239-5M, AM239-10M, AX239-YCD, MU239-UC p53 Protein 16, 27, 49, 121, 190 AM319-5M, AX319-YCD, MU319-UC c-erbB-3 (HER-3) 13, 24, 45, 81 AM240-5M, AM240-10M, AX240-YCD, MU240-UC p53 Protein 16, 27, 49, 121, 190 AM320-5M, AX320-YCD, MU320-UC CD45RB 12, 45, 73, 186 AM241-5M, AX241-YCD, MU241-UC CD31 12, 44, 70, 185 AM322-5M, AM322-10M, AX322-YCD, MU322-UC CD3 (T Cell) 12, 23, 44, 63, 185 AM243-5M, AX243-YCD Dystrophin 14, 46, 92, 187 AM325-5M, AX325-YCD CD66 13, 45, 76, 186 AM244-5M, AX244-YCD Dystrophin 14, 47, 92, 187 CD30 (Ki-1 Antigen) AM246-5M, AM246-10M, AX246-YCD, MU246-UC 12, 24, 44, 70, 185 Cytokeratin 19 14, 25, 46, 89, 187 AM327-5M, AM327-10M, AX327-YCD AM252-5M, AM252-10M, AX252-YCD, MU252-UC Proliferating Cell Nuclear Antigen (PCNA) 16, 27, 49, 125, 190 AM328-5ME, AM32810ME, AX328-YCDE, MU328-UCE Progesterone Receptor (InSite®PR) 16, 27, 49, 125, 190 AM254-5M, MU254-UCE Cytomegalovirus (CMV) 46, 91 AM331-5M, AM331-10M, AX331-YCD, MU331-UC Myosin Heavy Chains, Smooth Muscle 16, 26, 49, 115 AM255-5M, AM255-10M, AX255-YCD, MU255-UC Cytokeratin 7 13, 25, 46, 86, 187 AM332-5M, AX332-YCD, MU332-UC Caldesmon, High MW, Smooth muscle 11, 43, 59 AM256-2ME, AM25610ME, AX256-YCDE, MU256-UCE Androgen Receptor 11, 23, 43, 53, 184 AM333-5M, AX333-YCD, MU333-UC Calponin 11, 43, 59, 184 AM258-5M, AX258-YCD, MU258-UC CD3 (T Cell) 12, 44, 63 AM336-5M, AX336-YCD, MU336-UC GLEPP-1 14, 47, 99 AM261-5M, AX261-YCD, MU261-UC CD8 (T Cell,Suppressor/ Cytotoxic) 12, 44, 65 AM337-5M, AM337-10M, AX337-YCD, MU337-UC Factor XIII Subunit A 14, 25, 47, 97, 188 AM265-5M, AX265-YCD, MU265-UC CD20 (B Cell) 12, 44, 68 AM338-5M, AX338-YCD, MU338-UC CD45 (Leukocyte common Antigen,LCA) 11, 43, 52 AM266-5M, AX266-YCD CD21 (B Cell) 12, 44, 68 AM351-5M, AM351-10M, AX351-YCD, MU351-UC CD30 (Ki-1 Antigen) 12, 24, 44, 70 AM268-5M, AX268-YCD, MU268-UC CD56 (Natural Killer Cell,NCAM) 13, 45, 75 AM354-5M, AX354-YCD, MU354-UC CD71 (Transferrin Receptor) 13, 45, 77 AM269-5M, AX269-YCD CD71 (Transferrin Receptor) 13, 45, 76 AM270-5M, AX270-YCD CD11b (C3bi Receptor) 12, 44, 66 AM355-5M, AM355-10M, AX355-YCD, MU355-UC CD99 (MIC2 Antigen) 13, 24, 45, 78 AM272-2ME, AM27210ME, AX272-YCDE, MU272-UCE Estrogen Receptor 14, 25, 47, 95, 188 AM356-5M, AM356-10M, AX356-YCD, MU356-UC Chromogranin A 13, 24, 45, 82, 187 AM357-5M, AX357-YCD, MU357-UC Cytokeratin, Pan 14, 46, 91 internationalcs@biogenex.com 211 Catalog Number Index Cat. No. Product Name Page Cat. No. Product Name Page AM358-5M, AX358-YCD, MU358-UC Mucin 2 (MUC2) 15, 48, 113 AM414-5M, AM414-10M, AX414-YCD, MU414-UC CD79a 13, 24, 45, 77, 186 AM359-5M, AX359-YCD bFGF (Basic Fibroblast Growth Factor) 11, 43, 57 AM416-5M, AM416-10M, AX416-YCD, MU416-UC CD68 13, 24, 45, 76, 186 AM361-5M, AM361-10M, AX361-YCD, MU361-UC Melan-A (MART-1) 15, 26, 48, 109, 189 AM418-5M, AM418-10M, AX418-YCD, MU418-UC P63 16, 27, 49, 121, 190 AM362-5ME, AX362YCDE, MU362-UCE Papillomavirus Type 16 (HPV16) 16, 49, 121 AM419-5M, AM419-10M, AX419-YCD, MU419-UC Mast Cell Tryptase 26, 48, 109, 189 AM363-5M, AM363-10M, AX363-YCD, MU363-UC Synaptophysin 17, 27, 50, 129, 191 AM421-5M, AM421-10M, AX421-YCD, MU421-UC CD4 12, 23, 44, 63, 185 AM365-5M, AM365-10M, AX365-YCD, MU365-UC Carcinoembryonic Antigen (CEA) 13, 23, 43, 61, 185 AM422-5M, AM422-10M, AX422-YCD, MU422-UC CD8 12, 23, 44, 64, 185 AM366-5M, AX366-YCD, MU366-UC IgM 15, 48, 104, 188 AM423-5M, AM423-10M, AX423-YCD, MU423-UC CD117 13, 24, 45, 79, 186 AM367-5M, AX367-YCD, MU367-UC IgG 15, 48, 104, 188 AM424-5M, AM424-10M, AX424-YCD, MU424-UC CA19-9 11, 23, 43, 58, 184 AM368-5ME, AM36810ME, AX368-YCDE, MU368-UCE Estrogen Receptor (InSite® ER) 14, 25, 47, 96, 188 AM429-5M, AM429-10M, AX429-YCD, MU429-UC CA125 11, 23, 43, 59, 184 AM369-5M, AM369-10M, AX369-YCD, MU369-UC 15, 26, 48, 106, 189 AM430-5M, AM430-10M, AX430-YCD, MU430-UC CD5 Kappa Light Chain 12, 23, 44, 64, 185 AM370-5M, AM370-10M, AX370-YCD, MU370-UC 15, 26, 48, 107, 189 AM431-5M, AM431-10M, AX431-YCD, MU431-UC CD35 Ki-67 Antigen, Proliferating Cell 12, 24, 44, 71, 185 AM371-5M, AM371-10M, AX371-YCD, MU371-UC CD45 Cocktail (Leukocyte Antigen, LCA) 12, 24, 45, 73, 186 AM432-5M, AM432-10M, AX432-YCD, MU432-UC Myf4 16, 26, 49, 114, 189 AM372-5M, AM372-10M, AX372-YCD, MU372-UC Cytokeratin cocktail, Broad Spectrum 14, 25, 46, 90, 187 AM433-5M, AM433-10M, AX433-YCD, MU433-UC Mesothelin 15, 26, 48, 110, 189 AM373-5M, AM373-10M, AX373-YCD, MU373-UC Terminal Deoxynucleotidyl Transferase (TdT) AM434-5M, AX434-YCD, MU434-UC p21/WAF1 16, 49, 119, 190 13, 27, 50, 130 AM374-5M, AX374-YCD, MU374-UC 12, 44, 67, 185 15, 48, 106 AM437-5M, AX437-YCD, MU437-UC CD16 J Chain AM376-5M, AX376-YCD, MU376-UC Platelet-Derived Growth Factor (PDGF) AM438-5M, AX438-YCD, MU438-UC CD2 11, 44, 62, 185 16, 49, 123 AM377-5M, AX377-YCD, MU377-UC Transforming Growth Factor (TGF),Alpha AM439-5M, AX439-YCD, MU439-UC CD22 12, 44, 69, 185 17, 50, 133 AM379-5M, AM379-10M, AX379-YCD, MU379-UC 13, 45, 79, 186 14, 46, 83 AM441-5M, AX441-YCD, MU441-UC CD105 Collagen IV AM380-5M, AM380-10M, AX380-YCD 16, 26, 48, 113 AM446-5M, AM446-10M, AX446-YCD, MU446-UC Inhibin-Alpha Myelin Basic Protein 15, 26, 48, 105, 189 AM381-5M, AX381-YCD, MU381-UC Muscle Actins 16, 48, 113 AM448-5ME, AM44810ME, AX448-YCDE, MU448-UCE Mouse 4A4+ Mouse34BE12+ Rabbit13H4 16, 27, 147,190 AM382-5M, AX382-YCD, MU382-UC Aberrant Endothelial Cell 11, 43, 51 AM451-5M, AM451-10M, AX451-YCD, MU451-UC CD10 12, 23, 44, 65, 185 AM387-5M, AX387-YCD, MU387-UC Osteonectin 16, 49, 118 AM453-5M, AM453-10M, AX453-YCD, MU453-UC CD25 12, 24, 44, 69, 185 AM388-5M, AX388-YCD, MU388-UC Alpha-1-Antichymotrypsin 11, 43, 52 AM454-5M, AM454-10M, AX454-YCD, MU454-UC MSH6 15, 26, 48, 112 ,189 AM390-5M, AM390-10M, AX390-YCD, MU390-UC E-Cadherin 14, 25, 47, 93 AM455-5M, AM455-10M, AX455-YCD, MU455-UC MUC4 15, 26, 48, 112, 189 AM391-5M, AX391-YCD, MU391-UC Multi-Drug Resistance Marker (P-Glycoprotein) 15, 48, 113 AM456-5M, AM456-10M, AX456-YCD, MU456-UC MUC5AC 15, 26, 48, 112, 189 AM392-5M, AM392-10M, AX392-YCD, MU392A-UC CDX-2 13, 24, 45, 81, 186 AM457-5M, AM457-10M, AX457-YCD, MU457-UC PAX-5 16, 27, 49, 122, 190 AM395-5M, AX395-YCD, MU395-UC Human Chorionic Gonadotropin (hCG) Beta 15, 48, 103 AM459-5M, AM459-10M, AX459-YCD, MU459-UC Tau 17, 27, 50, 129, 191 AM396-5M, AX396-YCD, MU396-UC p27 (Kip1) 16, 49, 119 AM467-5M, AX467-YCD, MU467-UC Cathepsin D 11, 44, 62 AM397-5M, AM397-10M, AX397-YCD, MU397-UC TTF-1 17, 27, 50, 131, 191 AM487-5M, AM487-10M, AX487-YCD, MU487-UC ACTH 11, 23, 43, 51, 184 AM410-5M, AM410-10M, AX410-YCD, MU410-UC Ki-67 15, 26, 48, 106, 189 AM488-5M, AM488-10M, AX488-YCD, MU488-UC Fascin 14, 25, 47, 97, 188 AM412-5M, AM412-10M, AX412-YCD, MU412-UC Tau 17, 27, 50, 129, 191 AM490-5M, AM490-10M, AX490-YCD, MU490-UC CD1a 11, 23, 44, 62, 185 AM505-5M, AM505-10M, AX505-YCD, MU505-UC GLUT-1 14, 25, 47, 100, 188 212 www.biogenex.com Catalog Number Index Cat. No. Product Name Page Cat. No. Product Name Page AM511-5M, AM511-10M, AX511-YCD, MU511-UC Sarcomeric Actin 17, 27, 50, 128, 190 AN471-5ME, AN47110ME, AX471-YCDE ErbB-2/HER-2 13, 24, 47, 95 AM525-5M, AM525-10M, AX525-YCD, MU525-UC Villin 17, 27, 50, 134, 191 EGFR(PAN) 14, 25, 47, 93 AM529-5M, AM529-10M, AX529-YCD, MU529-UC AN473-5ME, AN47310ME, AX473-YCDE, NU473-UCE TIA -1 17, 27, 50, 132 13, 24, 46, 84, 187 PAP 16, 27, 49, 121, 190 AN474-5M, AN474-10M, AX474-YCD, NU474-UC Cyclin D1 AM532-5M, AM532-10M, AX532-YCD, MU532-UC CD3 Epsilon AM534-5M, AM534-10M, AX534-YCD, MU534-UC 12, 23, 44, 62, 185 CD227 (MUCIN 1) 15, 26, 45, 80, 186 AN477-5M, AN477-10M, AX477-YCD, NU477-UC AN480-5M, AN480-10M, AX480-YCD, NU480-UC Stat 5-Alpha (C-term) 17, 27, 50, 128,191 Tyrosinase 17, 27, 50, 133, 191 AN481-5M, AN481-10M, AX481-YCD, NU481-UC GCDFP-15 14, 25, 47, 98, 188 Melanoma gp100 15, 26, 48, 110, 189 13, 24, 45, 75, 186 CD20 12, 24, 44, 67, 185 AN482-5M, AN482-10M, AX482-YCD, NU482-UC CD61 AM537-5M, AM537-10M, AX537-YCD, MU537-UC Cytokeratin 5 13, 25, 46, 85 AM539-5M, AM539-10M, MU539-UC AN484-5M, AN484-10M, AX484-YCD, NU484-UC Glypican-3 (GPC3) 25, 47, 100, 188 AN485-5M, AN485-10M, AX485-YCD, NU485-UC Cytokeratin 15 14, 25, 46, 88 AM540-5M, AM540-10M, MU540-UC p16 (INK4a) 26, 49, 118, 190 AN486-5M, AN486-10M, AX486-YCD, NU486-UC Cytokeratin 16 14, 25, 46, 88 RCC (Renal Cell Carcinoma) 17, 27, 50, 126, 190 AN489-5M, AN489-10M, AX489-YCD, NU489-UC Ep-CAM 14, 25, 47, 94, 188 ZAP-70 17, 27, 50, 135, 191 16, 26, 49, 119, 190 CD68 13, 24, 45, 76, 186 AN492-5M, AN492-10M, AX492-YCD, NU492-UC p27 (Kip1) AM549-5M, AM549-10M, AX549-YCD, MU549-UC CD21 AM550-5M, AM550-10M, AX550-YCD, MU550-UC 12, 24, 44, 68, 185 HSA 15, 25, 48, 103, 188 AN497-5M, AN497-10M, AX497-YCD, NU497-UC AN500-5M, AN500-10M, AX500-YCD, NU500-UC Cytokeratin 6 13, 25, 46, 85, 187 MiTF 15, 26, 48, 110, 189 AN502-5M, AN502-10M, AX502-YCD, NU502-UC Calponin-1 (C-term) 11, 23, 43, 60, 184 DOG1 14, 25, 46, 92, 187 17, 27, 50, 129, 191 Cytokeratin 17 14, 25, 46, 88, 187 AN503-5M, AN503-10M, AX503-YCD, NU503-UC Survivin AM572-5M, AM572-10M, AX572-YCD, MU572-UC MMP-9 AM583-5M, AM583-10M, AX583-YCD, MU583-UC 15, 26, 48, 111, 189 Calretinin 11, 23, 43, 60, 184 AN504-5M, AN504-10M, AX504-YCD, NU504-UC 11, 23, 43, 59, 184 Cytokeratin 7&8 14, 25, 46, 86, 187 AN507-5M, AN507-10M, AX507-YCD, NU507-UC Caldesmon AM587-5M, AM587-10M, AX587-YCD, MU587-UC AM6010 TO AM7990 AR6010 TO AR6600 Estrogen Receptor (ER Alpha) 14, 25, 47, 96 Slide Barcode Labels 29 AN509-5ME, AN50910ME, AX509-YCDE, NU509-UCE AM701-5M, AM701-10M, AY701-YCD, MU701-UC Napsin A 16, 26, 49, 116, 189 AN510-5M, AN510-10M, AX510-YCD, NU510-UC Beta-Catenin 11, 23, 43, 56 ,184 AM702-5M, AM702-10M, AY702-YCD, MU702-UC CD7 12, 23, 44, 64, 185 AN512-5M, AN512-10M, AX512-YCD, NU512-UC CD5 12, 23, 44, 64, 185 AM703-5M, AM703-10M, AY703-YCD, MU703-UC MLH1 15, 26, 48, 111, 189 AN513-5M, AN513-10M, AX513-YCD, NU513-UC CD8 (C-Term) 12, 23, 44, 65, 185 AM705-5M, AM705-10M, AY705-YCD, MU705-UC Cytokeratin 4 13, 25, 46, 84, 187 AN515-5M, AN515-10M, AX515-YCD, NU515-UC CD14 12, 24, 44, 65, 185 AM708-5M, AM708-10M, AY708-YCD, NU708-UC Bcl-6 11, 23, 43, 55 AN516-5M, AN516-10M, AX516-YCD, NU516-UC Factor XIII A 14, 25, 47, 97, 188 AM736-5ME, AM73610ME, AY736-YCDE, MU736-UCE 16, 27, 49, 123 AN518-5M, AN518-10M, AX518-YCD, NU518-UC CD99 PGP9.5 13, 24, 45, 78, 186 Cyclin E2 AN449-5ME, AN44910ME, AX449-YCDE, NU449-UCE AN519-5M, AN519-10M, AX519-YCD, NU519-UC 13, 25, 46, 84, 187 P504S (AMACR) 16, 26, 49, 120, 190 AN520-5M, AN520-10M, AX520-YCD, NU520-UC TFPU.1 17, 27, 52, 130, 191 AN462-5M, AN462-10M, AX462-YCD, NU462-UC Bcl-x 11, 23, 43, 55, 184 AN521-5M, AN521-10M, AX521-YCD, NU521-UC Paxillin (N-Term) 16, 27, 49, 122, 190 AN463-5M, AN463-10M, AX463-YCD, NU463-UC PDGF Receptor-ß 16, 27, 49, 122, 190 AN522-5M, AN522-10M, AX522-YCD, NU522-UC NGF Receptor 16, 26, 49, 117, 190 AN465-5M, AN465-10M, AX465-YCD, NU465-UC CD117 13, 24, 45, 79, 186 AN523-5M, AN523-10M, AX523-YCD, NU523-UC Catenin (p120) 16, 26, 44, 61, 185 AN466-5M, AN466-10M, AX466-YCD, NU466-UC ZAP-70 17, 27, 50, 135, 191 AN524-5M, AN524-10M, AX524-YCD, NU524-UC PDCD4 16, 27, 49, 122, 190 AM535-5M, AM535-10M, AX535-YCD, MU535-UC AM536-5M, AM536-10M, AX536-YCD, MU536-UC AM543-5M, AM543-10M, AX543-YCD, MU543-UC AM544-5M, AM544-10M, AX544-YCD, MU544-UC AM554-5M, AM554-10M, AX554-YCD, MU554-UC AM570-5M, AM570-10M, AX570-YCD, MU570-UC internationalcs@biogenex.com 213 Catalog Number Index Cat. No. Product Name Page Cat. No. Product Name Page AN527-5M, AN527-10M, AX527-YCD, NU527-UC Ki-67 15, 26, 48, 107, 189 AN724-5M, AN724-10M, AY724-YCD, NU724-UC Oct 4 16, 28, 49, 118, 190 AN528-5M, AN528-10M, AX528-YCD, NU528-UC Cytokeratin 7 13, 25, 46, 86, 187 AN725-5M, AN725-10M, AY725-YCD, NU725-UC E-Cadherin 14, 25, 47, 93, 187 AN538-5M, AN538-10M, AX538-YCD, NU538-UC P504S (AMACR) 16, 26, 49, 120, 190 c-erbB-2 (HER-2/neu) 13, 24, 45, 81 AN541-5M, AN541-10M, AX541-YCD, NU541-UC Bcl-2 11, 23,43, 54, 184 AN726-5ME, AN72610ME, AY726-YCDE, NU726-UCE Ki-67 AN546-5M, AN546-10M, AX546-YCD, NU546-UC 14, 25, 48, 95, 188 CD11b/ITAM 12, 24, 44, 66, 185 AN727-5M, AN727-10M, AY727-YCD, NU727-UC AN728-5M, AN728-10M, AY728-YCD, NU728-UC p53 16, 26, 49, 120, 190 CD11c 12, 24, 44, 66, 185 AN729-5M, AN729-10M, AY729-YCD, NU729-UC CD19 12, 24, 44, 67, 185 Cytokeratin 20 14, 25, 46, 89, 187 AN558-5M, AN558-10M, AX558-YCD, NU558-UC AN730-5ME, AN73010ME, AY730-YCDE, NU730-UCE Cytokeratin 5 & Cytokeratin 14 13, 25, 46, 85 Oct 2 16, 26, 49, 117, 190 AN564-5M, AN564-10M, AX564-YCD, NU564-UC Mucin 1 (MUC1) 15, 26, 48, 112, 189 AN731-5ME, AN73110ME, AY731-YCDE, NU731-UCE BOB1 11, 23, 43, 58 AN565-5M, AN565-10M, AX565-YCD, NU565-UC N-Cadherin 16, 26, 49, 116, 189 CD41/Integrin Alpha IIb 12, 24, 44, 71 AN567-5M, AN567-10M, AX567-YCD, NU567-UC Apolipoprotein E 11, 23, 43, 53, 184 AN732-5ME, AN73210ME, AY732-YCDE, NU732-UCE AN569-5M, AN569-10M, AX569-YCD, NU569-UC 14, 25, 47, 98, 188 AN733-5M, AN733-10M, AY733-YCD, NU733-UC CD90 Fibronectin 13, 24, 45, 78, 186 AN576-5M, AN576-10M, AX576-YCD, NU576-UC 17, 27, 50, 132, 191 AN734-5M, AN734-10M, AY734-YCD, NU734-UC CD53 13, 24, 45, 74, Topo 2 Alpha AN735-5M, AN735-10M, AY735-YCD, NU735-UC Insulin PMS2 16, 27, 49, 124, 190 15, 26, 48, 106, 189 AN584-5M, AN584-10M, AX584-YCD, NU584-UC Wilm's Tumor 17, 27, 50, 135, 191 AN737-5ME, AN73710ME, AY737-YCDE, NU737-UCE CD205 13, 24, 45, 80 AN588-5M, AN588-10M, AX588-YCD, NU588-UC Cytokeratin 5 & 6 13, 25, 46, 85, 187 AN738-5M, AN738-10M, AY738-YCD, NU738-UC NGF Receptor 16, 26, 49, 117, 190 AN710-5ME, AN71010ME, AY710-YCDE, NU710-UCE Estrogen Receptor-Alpha 14, 25, 47, 96 AN739-5ME, AN73910ME, AY739-YCDE, NU739-UCE CD103 13,24, 45, 79 AN711-5ME, AN71110ME, AY711-YCDE, NU711-UCE Progesterone Receptor 16, 27, 49, 124 AN740-5M, AN740-10M, AY740-YCD, NU740-UC CD8 12, 23, 44, 65 AN712-5M, AN712-10M, AY712-YCD, NU712-UC AN741-5M, AN741-10M, AY741-YCD, NU741-YCD CD35 12, 24, 44, 71 S100P 17, 27, 50, 127, 190 AN713-5M, AN713-10M, AY713-YCD, NU713-UC 17, 27, 50, 127, 190 AN743-5M, AN743-10M, AY743-YCD, NU743-UC MSH2 15, 26, 48, 111 S100 Beta AR009-5R, AW009-YCD Carcinoembryonic Antigen (CEA) 11, 43, 61, 185 AN714-5M, AN714-10M, AY714-YCD, NU714-UC PMSA 17, 27, 50, 126, 190 AR012-5R,AW012-YCD, PU012-UP Myoglobin 16, 49, 115, 189 AN715-5M, AN715-10M, AY715-YCD, NU715-UC Lambda Light Chain 15, 26, 48, 108, 189 AR015-5R, AW015-YCD, PU015-UP Alpha-1-Antitrypsin 11, 43, 52, 184 AN716-5M, AN716-10M, AY716-YCD, NU716-UC CD146 13, 24, 45, 80, 186 AR019-5R, AW019-YCD, PU019-UP Gastrin 14, 47, 98, 188 AN717-5M, AN717-10M, AY717-YCD, NU717-UC Cytokeratin 4 13, 25, 46, 84, 187 AR020-5R, AR020-10R, AW020-YCD, PU020-UP Glial Fibrillary Acidic Protein (GFAP) 14, 47, 99, 188 Claudin-5 13, 24, 45, 82, 187 AR021-5R, AW021-YCD AN555-5M, AN555-10M, AX555-YCD, NU555-UC AN557-5M, AN557-10M, AX557-YCD, NU557-UC AN577-5M, AN577-10M, AX577-YCD, NU577-UC AN718-5M, AN718-10M, AY718-YCD, NU718-UC AN719-5M, AN719-10M, AY719-YCD, NU719-UC CD79a 13, 24, 45, 77, 186 AN720-5ME, AN72010ME, AY720-YCDE, NU720-UCE CD63 13, 24, 45, 75, 186 AN721-5ME, AN72110ME, AY721-YCDE, NU721-UCE CD48 AN722-5M, AN722-10M, AY722-YCD, NU722-UC CD4 12, 23, 44, 63, 185 AN723-5M, AN723-10M, AY723-YCD, NU723-UC Bcl-2 11, 23, 43, 55, 184 214 13, 24, 45, 74 Hemoglobin A 15, 47, 102, 188 AR024-5R, AR024-10R, AW024-YCD, PU024-UP Lysozyme 15, 26, 48, 108, 189 AR026-5R, AW026-YCD Follicle Stimulating Hormone (FSH) 14, 47 AR033-5R, AW033-YCD Thyroid Stimulating Hormone (TSH) 17, 50, 131 AR038-5R, AW038-YCD Estradiol 14, 47, 95, 188 AR039-5R, AW039-YCD, PU039-UP Glucagon 14, 47, 99, 188 AR040-5R, AR040-10R, AW040-YCD, PU040-UP Placental Lactogen (hPL) 16, 27, 49, 123, 190 AR045-5R, AW045-YCD, PU045-UP IgA 15, 48, 103 www.biogenex.com Catalog Number Index Cat. No. Product Name Page Cat. No. Product Name Page AR049-5R,AW049-YCD, PU049-UP Lambda Light Chain 15, 48, 108, 189 B AR050-5R, AW050-YCD, PU050-UP IgG 15, 48, 104, 188 BLS500 i500 S Bar Code Labeling System 10, 30 AR058-5R, AR058-10R, AW058-YCD, PU058-UP S-100 Protein 17, 27, 50, 127, 190 BS-1310-25 Power Block Reagent, Readyto-use 177 AR067-5R, AW067-YCD Secretin 17, 50, 128, 191 AR069-5R, AW069-YCD, PU069-UP D Substance P 17, 50, 128, 191 DF400-50KE 158 AR078-5R,AR078-10R, AW078-YCD, PU078-UP Laminin 15, 26, 48, 108, 189 Super Sensitive One Step Polymer-HRP ISH Detection System Hepatitis B Virus Core Antigen (HBcAg) DF400-YADE 47, 102 XISH One Step Polymer-HRP ISH Detection System 19, 158 AR082-5RE, PU082-UPE DF500-20XE eFISH Histo Kit 163 AR084-5RE, AW084YCDE, PU084-UPE Herpes Simplex Virus Type I (HSV I) 15, 47, 102 DF510-20XE eFISH Cyto Kit 163 DF530-40KE eFISH U Kit 163 EK000-10K, EK000-10X Pepsin 4-Pack Kit 19, 30 EK000-5K Pepsin 3-Pack Kit 38 AR085-5RE, AW085YCDE, PU085-UPE Herpes Simplex Virus Type II (HSV II) 15, 47, 102 AR125-5RE, PU125-UPE Toxoplasma gondii 50, 132 AR249-5R, AW249-YCD, PU249-UP Glutathione S-Transferase Pi (GST Pi) 14, 47, 100, 188 AR335-5RE, AW335YCDE, PU335-UPE EGFR 14, 47, 93 AR345-5R, AW345-YCD, PU345-UP BRCA1 Protein 11, 43, 55 E EK001-10K, EK001-10X Trypsin 4-Pack Kit 19, 30 EK001-5K Trypsin 3-Pack Kit 38 EK002-10K, EK002-10X Protease XXIV 4-Pack Kit 19, 30 EK002-5K Protease XXIV 3-Pack Kit 38 EK004-5K Diastase (a-Amylase) 30, 38 Positive Control on Barrier Slides 184 - 191 AR347-5R, AW347-YCD, PU347-UP Bax Protein 11, 43, 54 AR373-5R,AW373-YCD Terminal Deoxynucleotidyl Transferase (TdT) 17, 50, 130, 191 F AR376-5R, AW376-YCD, PU376-UP Platelet-Derived Growth Factor (PDGF) 16, 49, 124 FB-001M to FB-743N AR385-5R, AR385-10R, AW385-YCD, PU385-UP Estrogen Receptor-Beta (ER-β) 14, 25, 47, 96 FG-001M to FG-743N Positive Control Slides 184 - 191 eFISH MDM2/CEN 12 Dual Color Probe 162 AR413-5R, AR413-10R, AW413-YCD, PU413-UP Calretinin 11, 23, 60, 184 FP038-10XE, FP03820XE AR427-5R,AR427-10R, AW427-YCD, PU427-UP IgM 15, 48, 105, 188 FP039-10XE, FP03920XE eFISH CEN 17/HER2 Dual Color Probe 162 AR440-5R, AR440-5RSG, PU440-UP, AW440-YCD lgD 15, 48, 104, 188 FP040-10XE, FP04020X E eFISH EGFR/CEN 7 Dual Color Probe 162 AR442-5RE,AR44210RE, AW442-YCDE, PU442-UPE 15, 26, 47, 101 FP041-10XE , FP04120XE eFISH p16/CEN 9 Dual Color Probe 162 Helicobacter pylori FP042-10XE, FP04220XE eFISH FGFR1/CEN 8 Dual Color Probe 162 Cyclin D1 13, 24, 46, 83, 187 eFISH NMYC/2q11 Dual Color Probe 162 CD23 12, 24, 44, 69, 185 FP043-10XE, FP04320XE FP044-10XE, FP04420XE eFISH 1p36/1q25 Dual Color Probe 162 VEGF 17, 27, 50, 134, 191 FP045-10XE, FP04520XE eFISH 19q13/19p13 Dual Color Probe 162 Myeloperoxidase (MPO) 16, 26, 49, 114, 189 FP046-10XE, FP04620XE eFISH VHL/CEN 3 Dual Color Probe 162 VIP 17, 27, 50, 135, 191 eFISH MET/CEN 7 Dual Color Probe 162 Claudin 1 13, 24, 45, 82, 187 FP047-10XE, FP04720XE eFISH EWSR1 Dual Color Break Apart Probe 162 Catenin Delta 1 (p120) 11, 23, 44, 61, 185 FP048-10XE, FP04820XE eFISH SYT Dual Color Break Apart Probe HGH 15, 26, 47, 101, 188 FP049-10XE, FP04920XE 162 AR707-5R, AR707-10R, AW707-YCD, PU707-UP FP050-10X, FP050-20XE Xmatrx Nano 4, 7, 11 eFISH CHOP Dual Color Break Apart Probe 162 AS1000 AS1010 Xmatrx Mini 5, 7, 11 FP051-10XE , FP05120XE eFISH TFE3 Dual Color Break Apart Probe 162 AS4000RX Xmatrx Infinity 6, 11 AS4040B Xmatrx Elite 2, 7, 11 FP052-10XE, FP05220XE eFISH COL1A1/PDGFB Dual Color Dual Fusion Probe 162 AS6030 i6000 Diagnostic 8, 23 FP053-10XE , FP05320XE eFISH PDGFB Dual Color Break Apart Probe 162 AS6040 i6000 Infinity 9, 23 AR447-5R, AR447-10R, AW447-YCD, PU447-UP AR460-5R,AR460-10R, AW460-YCD, PU460-UP AR483-5R, AR483-10R, AW483-YCD, PU483-UP AR496-5R, AR496-10R, AW496-YCD, PU496-UP AR530-5R,AR530-10R, AW530-YCD, PU530-UP AR704-5R, AR704-10R, AW704-YCD, PU704-UP AR706-5R, AR706-10R, AW706-YCD, PU706-UP internationalcs@biogenex.com 215 Catalog Number Index Cat. No. Product Name Page FP054-10XE, FP05420XE eFISH COL1A1 Dual Color Break Apart Probe 162 FP055-10XE, FP05520XE eFISH FGFR2/CEN 10 Dual Color Probe FP056-10XE, FP05620XE Product Name Page HK099-5K Aqueous Mounting Medium 180 HK100-5K, HK100-9K Hematoxylin 180 162 HK102-5K, HK102-10K, HK102-20X Avidin Biotin Blocking Kit 28, 177 eFISH ALK Dual Color Break Apart Probe 162 HK111-5K, HK111-50K Peroxide Block 177 FP057-10XE, FP05720XE eFISH CCND1/IGH Dual Color Dual Fusion Probe HK112-5K, HK112-9KE Blocking Reagent II Goat Serum 177 162 HK114-5K 177 FP058-10XE, FP05820XE eFISH FUS Dual Color Break Apart Probe Blocking Reagent II Rabbit Serum 162 HK119-7M SS Mouse Negative Control 179 FP059-10XE, FP05920XE eFISH PIK3CA/CEN 3 Dual Color Probe 162 HK124-7K, HK124-9K Liquid DAB Chromogen 178 FP060-10XE, FP06020XE eFISH ROS1 Dual Color Break Apart Probe HK129-5KE AEC Substrate Kit, Conc 178 162 HK139-06K AEC Chromogen 178 FP061-10XE, FP06120XE eFISH RET Dual Color Break Apart Probe HK139-50KE One Step AEC Solution 178 HK144-5KE Elegance Red Substrate Pack 179 FP062-10XE, FP06220XE eFISH TP53/CEN 17 Dual Color Probe 162 HK156-5KE Common Ab Diluent 176 FP063-10XE, FP06320XE eFISH CCND1/CEN 11 Dual Color Probe HK157-5KE S-A HRP Diluent 176 162 HK165-5KE Link Diluent 176 FP064-10XE, FP06420XE eFISH CMYC Dual Color Break Apart Probe 162 HK169-5K H & E Retrieval Solution (10X) 37 HK182-5KE Fast Red Substrate Pack 179 FP065-10XE, FP06520XE eFISH CMYC/CEN 8 Dual Color Probe 162 HK183-5KE New Fuchsin Substrate Pack 179 FP066-10XE, FP06620XE eFISH TERT/5q31 Dual Color Probe HK268-UK Conc Multilink 142 HK320-UK Conc Strept Label HRP 142 FP067-10XE, FP06720XE eFISH CMYC/IGH Dual Color Dual Fusion Probe HK321-UK Conc Strept Label AP 142 HK325-UM Conc Mouse Link 142 HK326-UR Conc Rabbit Link 142 HK327-UG Conc Goat Link 142 HK330-5K, HK330-9K Super Sensitive HRP Label 142 HK331-5K, HK331-9K SS Strept Alka Phos Label 142 HK335-5M, HK335-9M SS Mouse Link 142 HK336-5R, HK336-9R SS Rabbit Link 142 HK337-5G SS Goat Link 142 HK338-5T SS Rat Link 142 HK340-5K, HK340-9K SS Multilink 142 HK406-5G Goat, SS Neg Control 179 HK407-5T Rat, SS Neg Control 179 HK408-5R, HK408-7R Rabbit, SS Neg Control 179 HK521-XAK , HK521-XIK EZ-AR1, RTU 30, 35 HK522-XAK , HK522-XIK EZ-AR2, RTU 30, 35 HK542-XAK DAB 2-Component Pack 178 HK543-YOK EZ-AR3 30, 35 HK544-YOK EZ-AR4 30, 35 HK545-XOK EZ-AR Common 30, 35 HK546-XAK EZ-AR1 Elegance, RTU 34 HK547-XAK EZ-AR2 Elegance, RTU 34 30, 176 162 162 162 FP068-10XE, FP06820XE eFISH EGR1/5p15 Dual Color Probe FP069-10XE , FP06920XE eFISH CCND1 Dual Color Break Apart Probe 162 FP070-10XE , FP07020XE eFISH IGH Dual Color Break Apart Probe 162 FP071-10XE , FP07120XE eFISH BCR/ABL Dual Color Dual Fusion Probe 162 FP072-10XE, FP07220XE eFISH AML1/ETO Dual Color Dual Fusion Probe 162 FP073-10XE , FP07320XE eFISH PML/RARA Dual Color Dual Fusion Probe 162 FP074-10XE, FP074-20XE eFISH BCL2/IGH Dual Color Dual Fusion Probe 162 eFISH BIRC3/MALT1 Dual Color Dual Fusion Probe 162 FP075-10XE, FP07520XE FP076-10XE, FP076-20XE eFISH ETV6/RUNX1 Dual Color Dual Fusion Probe 162 162 H HK057-5KE Antigen Retrieval AR-10 (10X) 37 HK058-5K, HK058-20K Antigen Retrieval AR-10 RTU 37 HK079-5K, HK079-7K Super Mount 180 HK080-5K, HK080-9K Antigen Retrieval Citra Plus (10X) 36 Cat. No. HK081-5KE, HK08120KE Antigen Retrieval Citra Plus RTU 36 HK583-5KE Super Sensitive Wash Buffer, 20X HK085-5KE Power Block (10X) 177 HK584-5KE EZ-DeWax Solution, Conc 29, 32 HK086-5K, HK086-9K Antigen Retrieval Citra (10X) 36 HK941-YAKE Enhanced Antibody Diluent 176 HK972-YRXE AutoFISH Reagent A 163 HK973-YRXE AutoFISH Reagent B 163 HK087-5KE, HK08720KE Antigen Retrieval Citra RTU 36 HK091-5KE Phosphate Buffer Saline (PBS) 176 HK981-YRXE AutoFISH Protease 1 Buffer 163 HK092-5K AEC Substrate Pack Kit 178 HK982-YRXE AutoFISH Protease 2 Buffer 163 HK098-5K Tris Buffer, Conc pH 7.6 176 216 www.biogenex.com Catalog Number Index Cat. No. Product Name Page Cat. No. Product Name Page HK983-YRXE AutoFISH Pretreatment Reagent 163 MW001-HB Microwave AR Accessory Kit 34 HM001-100E Hsa-miR-1 Probe 152, 157 MW001-SU Microwave AR Single Access Kit 34 HM007A-100E Hsa-miR-7a Probe 156, 157 HM010B-100E Hsa-miR-10b Probe 152, 157 N HM017-100E Hsa-miR-17 Probe 152, 157 Nordic Ware Pressure Cooker 39 PR026-100E, PR026YADE ALU Probe II 19, 150, 151 PR031-100E U6 Probe 156, 157 PR032-100E Scramble Probe 156, 157 PR1055-100E, PR1055YADE Beta Actin 19, 150, 151 PR205-100E, PR205YADE EBER Probe 19, 150, 151 PR214-100E, PR214YADE Kappa Probe 19, 151 PR215-100E, PR215YADE Lambda Probe 19, 151 PR216-100E, PR216YADE Cyclin D1 Probe 19, 150, 151 PR217-100E, PR217YADE Oligo d(T) Probe 19, 151 PR225-100E, PR225YADE Retinoblastoma (RB) Probe 19, 151 PR250-100E, PR250YADE HPV 16/18 Probe 19, 150, 151 PR251-100E, PR251YADE HPV 14 Probe 19, 150, 151 HM021-100E Hsa-miR-21 Probe 153, 157 HM106A-100E Hsa-miR-106a Probe 153, 157 HM125B-100E Hsa-miR-125b Probe 153, 157 HM126-100E Hsa-miR-126 Probe 153, 157 HM1285-100E Hsa-miR-1285 Probe 156, 157 NW001-PC P HM141-100E Hsa-miR-141 Probe 153, 157 HM144-100E Hsa-miR-144 Probe 153, 157 HM146A-100E Hsa-miR-146a Probe 154, 157 HM147B-100E Hsa-miR-147b Probe 154, 157 HM150-100E Hsa-miR-150 Probe 154, 157 HM151-100E Hsa-miR-151a-3p Probe 154, 157 HM152-100E Hsa-miR-152 Probe 154, 157 HM155-100E Hsa-miR-155 Probe 154, 157 HM196A-100E Hsa-miR-196a Probe 155, 157 HM200A-100E Hsa-miR-200a Probe 152, 157 HM200B-100E Hsa-miR-200b Probe 152, 157 HM204-100E Hsa-miR-204 Probe 155, 157 HM205-100E Hsa-miR-205 Probe 155, 157 HM216A-100E Hsa-miR-216a Probe 155, 157 HM222-100E Hsa-miR-222 Probe 155, 157 HM328-100E Hsa-miR-328 Probe 156, 157 Q HM375-100E Hsa-miR-375 Probe 156, 157 QA900-9L Super Sensitive Link Label IHC Detection System, Alk Phos 141 HX016-XAK, HX016-XEK X-DeWax 21 HX017-YIK X-Wash ISH Wash Buffer 21 QD400-60KE Super Sensitive Non-Biotin HRP, DAB Kit 139 HX020-YIK X-Wash Buffer, 20X, for Xmatrx 21 QD410-YAXE EZ-AR™ 1 for Xmatrx 19 Super Sensitive Non-Biotin HRP, DAB Automated Kit 28 HX031-YCDE, HX031-YCX HX032-YCDE, HX032-YCX EZ-AR™ 2 for Xmatrx 19 QD420-YIKE Super Sensitive Link Label IHC Detection System, HRP/DAB 139 HX033-YCDE, HX033-YCX EZ-AR™ 3 for Xmatrx 19 HX034-YCDE, HX034-YCX EZ-AR™ 4 for Xmatrx 19 QD430-XAKE 139 HX035-04X, HX035-10X, HX035-40D Super Sensitive Link Label IHC Detection System, HRP/DAB Mega Xmount 21 QD440-XAK Super Sensitive Polymer-HRP IHC Kit 139 QD470-60K, QD470-YCX SS Multilink Kit,HRP/DAB 28, 141 QD550-YCDE, QD550-YCX XVIZ Detection Kit 18 QD610-YADE SS One Step Polymer-HRP IHC Detection System for Xmatrx 28 QD610-YAXE One-Step Polymer-HRP 28 QD620-YIKE One-Step Polymer-HRP 139 QD630-XAKE One-Step Polymer-HRP 139 QP000-5L, QP900-9L Super Sensitive Link HRP IHC Detection System 141 QP300-XAK Mega Multilink/HRP Kit 141 QS200-60K SS Double Stain Polymer Detection Kit I/DAB & Fast Red 144 QS200-YADE XVIZ Doublestaining Detection Kit I 18 QS210-YIK SS Double Stain Polymer Detection Kit I/Large Volume 144 QS400-60K SS Double Stain Polymer Detection Kit II/DAB & Fast Red 144 L LA000-ULE LP000-ULE Super Sensitive Link Label IHC Detection System, AP 141 Super Sensitive Link Label IHC Detection System, HRP 141 M MU121-UC Alpha-Tubulin 43, 53, 184 MU122-UC Beta-Tubulin 43, 56, 184 MU176-UC Beta-Tubulin II 43, 56, 184 MU177-UC Beta-Tubulin III 43, 56, 184 MU178-UC Beta-Tubulin IV 43, 57, 184 MU210-UC Granulocyte 47, 100, 188 MU213-UC Mitochondrial Antigen 48, 111 MU230-UC Nuclear Ribonucleoprotein 49, 117, 190 MU303-UC Blood Group Antigen Lewis A 43, 57, 184 MU304-UC Blood Group Antigen Lewis B 43, 57, 184 internationalcs@biogenex.com 217 Catalog Number Index Cat. No. Product Name Page Cat. No. Product Name Page QS400-YADE XVIZ Doublestaining Detection Kit II 18 XT026-V24 Unlabeled White Optimiser Vials with Caps and Holders, 20mL 28 QS410-YIK SS Double Stain Polymer Detection Kit II/Large Volume 144 XT027-H24 Optimiser Vial Holders (24) 28 XT034-XEX Resin Ribbon i500 PLUS 30 XT035-XBX Label Roll i500 PLUS 30 XT077-AX0601 to XT077AX0800 User Defined Empty RFID Tagged Vials Two Step IHC 21 XT077-AX0601 to XT0821-AX0999 User Defined Empty RFID Tagged Vials One Step IHC 21 XT077-V24 Empty Reagent Vials for Xmatrx Rx-White 21 XT078-V24 Empty Reagent Vials for Xmatrx Rx-Brown 21 Empty RFID Vial User Defined for ISH 20 28 S SS025-50X Acid Fast Blue Stain Kit 168, 173 SS026-50X, SS026-YAK Alcian Blue PAS Stain 168, 173, 174 SS027-50X, SS027-YAK Alcian Blue Stain 168, 173, 174 SS028-50X, SS028-YAK Congo Red Solution 168, 173, 174 SS029-50X Elastic Stain 169, 173 SS030-50X, SS030-YAK Iron Stain Kit 170, 173, 174 SS032-50X, SS032-YAK PAS Staining Kit 171, 173, 174 SS033-50X, SS033-YAK Gomoris Trichome Blue 169, 173, 174 XT079-PR0050 to XT079PR0099 SS034-50X, SS034-YAK Gomoris Trichome Greeen 170, 173, 174 XT101-24X Optimiser Reagent Vials, 20mL SS035-50X, SS035-YAK Masson's Trichrome Kit 170, 173, 174 XT104-05X i6000 Pipette Tips (960 tips) 20, 22, 29 SS036-50X, SS036-YAK Mucicarmine Stain 171, 173, 174 XT105-01X i6000 Pipette Tips (192 tips) 20, 22, 29 SS037-50X, SS037-YAK Gram Stain Kit 170, 173, 174 XT108-CL, XT108-SL Barrier Slides (25x25mm) 20, 22 SS038-50X, SS038-YAK Azure A satin 169, 173, 174 XT114-CL, XT114-SL Barrier Slides, 2 Zones, for Xmatrx 20, 22 SS039-50X, SS039-YAK Perodic Acid Schiff (PAS) Diastase Stain 171, 173, 174 XT118-50X, XT118-YRK Safranin O Staining Protocol 172, 173, 174 Coverslips, 25X40mm, for Xmatrx 20, 22 SS040-50X, SS040-YAK SS041-50X, SS041-YAK Sudan Black B Stain 172, 173, 174 XT121-XBK, XT121-YBX Coverslips, 18X18mm, for Xmatrx 20, 22 SS042-50X, SS042-YAK GMS for Fungi Stain Kit 170, 173, 174 XT122-90X, XT122-YQK Oil Red O Stain 169, 173, 174 Coverslips, 25X25mm, for Xmatrx 20, 22 SS043-50X, SS043-YAK XT126-24V Xmatrx Reagent Vials 20, 22 XT128-CL, XT128-SL Barrier Slides, 18X18mm, for Xmatrx 20, 22 XT132-YCD Xflex Consumable Kit, for Xmatrx 20 XT134-CL, XT134-SL Barrier Slides, 25X40mm, for Xmatrx 20, 22, 29, 181 XT144-YAD ISH Consumable Kit, for Xmatrx 20 XT145-05X Pipette Tips, 200ul (960 tips)/5 Boxes, for Xmatrx 21, 22 XT146-01X Pipette Tips, 200ul (192 tips), for Xmatrx 21, 22 XT147-YAD FISH Consumable Kit, for Xmatrx 20, 22 XT148-YCD Xflex Ultra Consumable Kit, for Xmatrx 20 XT149-V24 Reagent Vial Insert for RFID 21, 22 XT150-YCDE Auto DAB kit for Xmatrx Elite 18 SS044-50X, SS044-YAK Van Gieson Stain 172, 173, 174 SS045-50K von Kossa stain 173, 174 SS046-50X, SS046-YAK Reticulin/No Counterstain Stain Kit 172, 173, 174 SS047-50X, SS047-YAK Reticulin/No Counterstain Stain Kit 172, 173, 174 SS049-50X, SS049-YAK Geimsa Stain 169, 173, 174 SS050-50X, SS050-YAK Renal Masson's Trichome 171, 173, 174 SS052-50K Alizarin Red Stain 168, 174 SS053-50X, SS053-YAK PAS for Fungi Stain 171, 173, 174 SS054-50X, SS054-YAK Colloidal Iron stain 169, 173, 174 SS055-50X, SS055-YAK Bielschowsky's Silver Stain 169, 173, 174 SS057-50X, SS057-YAK Toluidine blue stain 172, 173, 174 SS058-50X, SS058-YAK Jones' Basement Membrane Stain 170, 173, 174 SS059-50X AFB Green 168, 173 XT001-PP PAP Pen 29, 181 XT002-CL, XT002-SL OptiPlus Microscope Slides 181 XT013-CL, XT013-SL OptiPlus Barrier Slides, 2/3 Test Area 29, 181 XT014-CL OptiPlus Barrier Slides, 3 X 1/3 Test Area,20 Boxes 29, 181 XT014-SL OptiPlus Barrier Slides, 3 X 1/3 Test Area,72 Slides 29, 181 XT022-CP Cap for Optimiser Vials (24) 28 XT026-601 to XT026799, XT026-601P to XT026-660P Optimiser Reagent Vial (20mL) 28 XT026-PR601 to XT026PR615 Reagent Empty Vial Labeled for User Probe 28 X 218 www.biogenex.com Antibody Clone Index Clone Antibody Cat. No. Page Clone Antibody Cat. No. Page 1.1 DOG1 AM570-5M, AM570-10M, AX570-YCD, MU570-UC 14, 25, 46, 92, 187 2G9A10F5 TIA -1 AM529-5M, AM529-10M, AX529-YCD, MU529-UC 17, 27, 50, 132 36 E-Cadherin AM390-5M, AM390-10M, AX390-YCD, MU390-UC 14, 25, 47, 93 2H11 Thyroglobulin AM032-5M, AM032-10M, AX032-YCD, MU032-UC 17, 27, 50, 130, 191 1801 p53 Protein AM240-5M, AM240-10M, AX240-YCD, MU240-UC 16, 27, 49, 121, 190 2H7 CD16 AM437-5M, AX437-YCD, MU437-UC 12, 44, 67, 185 AM033-5M, AM033-10M, AX033-YCD, MU033-UC 34βE12 Cytokeratin, High MW 17, 27, 50, 131 AM291-5M, AM291-10M, AX291-YCD 14, 25, 46, 90, 187 5404 Thyroid Stimulating Hormone (TSH) 34βE12/C51/AE1 14, 46, 90, 187 AM222-5ME, MU222-UCE AM273-5M, AX273-YCD 1108-1 Epstein-Barr Virus Early Antigen 47, 95, 188 Cytokeratin cocktail, Broad Spectrum 113-1 Mitochondrial Antigen MU213-UC 48, 111 3D9 PGP9.5 16, 27, 49, 123 p63 Protein AM418-5M, AM418-10M, AX418-YCD, MU418-UC AM736-5ME, AM73610ME, AY736-YCDE, MU736-UCE 16, 27, 49, 121, 190 45M1 MUC5AC 15, 26, 48, 112, 189 AM414-5M, AM414-10M, AX414-YCD, MU414-UC AM456-5M, AM456-10M, AX456-YCD, MU456-UC 13, 24, 45, 77, 186 4A11 Aberrant Endothelial Cell AM382-5M, AX382-YCD, MU382-UC 11, 43, 51 16, 26, 49, 120, 190 4B12 CD4 AM421-5M, AM421-10M, AX421-YCD, MU421-UC 12, 23, 44, 63, 185 4A4 11E 3 CD79a 13H4 P504S (AMACR) AN449-5ME, AN44910ME, AX449-YCDE, NU449-UCE 15E2E2 S-100 Protein AM058-5M, AM058-10M, AX058-YCD, MU058-UC 17, 27, 50, 127, 190 4C7 CD5 AM430-5M, AM430-10M, AX430-YCD, MU430-UC 12, 23, 44, 64, 185 1A4 Actin, Smooth Muscle AM128-5M, AM128-10M, AX128-YCD, MU128-UC 11, 23, 43, 51, 184 4C9 CD25 AM453-5M, AM453-10M, AX453-YCD, MU453-UC 12, 24, 44, 69, 185 1A5 CD8 AM422-5M, AM422-10M, AX422-YCD, MU422-UC 12, 23, 44, 64, 185 4D10 p21/WAF1 AM434-5M, AX434-YCD, MU434-UC 16, 49, 119, 190 4G11 CD105 AM441-5M, AX441-YCD, MU441-UC 13, 45, 79, 186 Progesterone Receptor AM172-2ME, AM17210ME, AX172-YCDE, MU172-UCE 16, 27, 49, 124 56C6 CD10 12, 23, 44, 65, 185 Estrogen Receptor AM272-2ME, AM27210ME, AX272-YCDE, MU272-UCE AM451-5M, AM451-10M, AX451-YCD, MU451-UC 14, 25, 47, 95, 188 58-15 Nuclear Ribonucleoprotein MU230-UC 49, 117, 190 5B2 Mesothelin MUC4 AM433-5M, AM433-10M, AX433-YCD, MU433-UC 15, 26, 48, 110, 189 1G8 AM455-5M, AM455-10M, AX455-YCD, MU455-UC 5C11 GLEPP-1 MU304-UC 43, 57, 184 AM336-5M, AX336-YCD, MU336-UC 14, 47, 99 2-25LE Blood Group Antigen Lewis B 2B3 p105 ProliferationAssociated Nuclear Antigen AM317-5M, AX317-YCD, MU317-UC 16, 49, 118 5D3 Cytokeratin 8 & 18 AM131-5M, AM131-10M, AX131-YCD, MU131-UC 14, 25, 46, 87, 187 2D4B5 MSH6 AM454-5M, AM454-10M, AX454-YCD, MU454-UC 15, 26, 48, 112,189 6B10 Cytokeratin 4 AM705-5M, AM705-10M, AY705-YCD, MU705-UC 13, 25, 46, 84, 187 2E7 Calretinin AM583-5M, AM583-10M, AX583-YCD, MU583-UC 11, 23, 43, 60, 184 7LE Blood Group Antigen Lewis A MU303-UC 43, 57, 184 1A6 1D5 internationalcs@biogenex.com 15, 26, 48, 112, 189 219 Antibody Clone Index Clone Antibody Cat. No. Page 8G7G3/1 TTF-1 AM397-5M, AM397-10M, AX397-YCD, MU397-UC 17, 27, 50, 131, 191 9E10 c-myc Protein AM318-5M, AX318-YCD, MU318-UC 13. 45, 83, 187 9G11 CD31 AM241-5M, AX241-YCD, MU241-UC 12, 44, 70, 185 A103 Melan-A (MART-1) AM361-5M, AM361-10M, AX361-YCD, MU361-UC 15, 26, 48, 109, 189 a1A88 Alpha-1Antichymotrypsin AM388-5M, AX388-YCD, MU388-UC A27-42 B Lymphocyte Antigen 36 (BLA.36) AM231-5M, AM231-10M, AX231-YCD, MU231-UC A40010 PAP AM532-5M, AM532-10M, AX532-YCD, MU532-UC 16, 27, 49, 121, 190 11, 43, 52 Adenovirus AA1 Mast Cell Tryptase AM419-5M, AM419-10M, AX419-YCD, MU419-UC 26, 48, 109, 189 AM438-5M, AX438-YCD, MU438-UC 11, 44, 62, 185 16, 48, 113 14, 25, 46, 91, 187 Actin 88 Cocktail Muscle Actins AM381-5M, AX381-YCD, MU381-UC AE1 Cytokeratin, Low MW AM075-5M, AM075-10M, AX075-YCD, MU075-UC AE1 & AE3 Cytokeratin Cocktail AM071-5M, AM071-10M, AX071-YCD, MU071-UC Cytokeratin, High MW (Basic) AM133-5M, AX133-YCD, MU133-UC 14, 46, 90, 187 AE8 Cytokeratin 13 AM132-5M, AX132-YCD 14, 46, 87, 187 AH26 ACTH AM487-5M, AM487-10M, AX487-YCD, MU487-UC 11, 23, 43, 51, 184 11, 23, 43, 60, 185 B01-94-11M-P Carcinoembryonic Antigen (CEA) B01-94-21M-NA Prostate Specific Acid Phosphatase (PSAP) AM013-5ME, AM01310ME, AX013-YCDE, MU013-UCE 16, 27, 50, 125, 190 B1 CD20 (B Cell) AM265-5M, AX265-YCD, MU265-UC 12, 44, 68 B2 CD21 (B Cell) AM266-5M, AX266-YCD 12, 44, 68 220 Page B6.2 Tumor-Associated Glycoprotein (TAG90 BCA) AM005-5M, AX005-YCD, MU005-UC 17, 50, 133, 191 B72.3 Tumor-Associated Glycoprotein (TAG-72) AM054-5M, AM054-10M, AX054-YCD, MU054-UC 17, 27, 50, 133, 191 Bcl-2/100 Bcl-2 Oncoprotein AM287-5M, AM287-10M, AX287-YCD 11, 23, 43, 55, 184 Ber-H2 CD30 (Ki-1 Antigen) AM327-5M, AM327-10M, AX327-YCD 12, 24, 44, 70, 185 bFGF88 bFGF (Basic Fibroblast Growth Factor) AM359-5M, AX359-YCD 11, 43, 57 BM-1 Myeloid specific Antigen AM164-5M, MU164-UC, AX164-YCD 16, 48, 114, 189 BM-2 Granulocyte MU210-UC 47, 100, 188 BM204 Cytomegalovirus (CMV) AM254-5M, MU254-UCE 46, 91 BM-3 Myeloid specific Antigen AM216-5M, AX216-YCD 16, 48, 114 BP53-12-1 p53 Protein AM195-5M, AM195-10M, AX195-YCD, MU195-UC 16, 26, 49, 120, 190 BRA4F1 CD15 AM302-5M, AM302-10M, AX302-YCD, MU302-UC 12, 24, 44, 67, 185 BRM-22 Heat Shock Protein (HSP-70) AM289-5M, AX289-YCD, MU289-UC 15, 47, 101, 188 BY114 CD66 AM325-5M, AX325-YCD 13, 45, 76, 186 C11 Cytokeratin, Pan AM357-5M, AX357-YCD, MU357-UC 14, 46, 91 C15 Cathepsin D AM467-5M, AX467-YCD, MU467-UC 11, 44, 62 C241:5:1:4 CA19-9 AM424-5M, AM424-10M, AX424-YCD, MU424-UC 11, 23, 43, 58, 184 C3 Alpha-Fetoprotein (AFP) AM008-5M, AM008-10M, AX008-YCD, MU008A-UC 11, 23, 43, 53, 184 C51 Cytokeratin 8 AM142-5M, AM142-10M, AX142-YCD, MU142-UC 14, 25, 46, 86, 187 CALP Calponin AM333-5M, AX333-YCD, MU333-UC 11, 43, 59, 184 Cam Vir-1 Papillomavirus Type 16 (HPV-16) AM362-5ME, AX362-YCDE, MU362-UCE 16, 49, 121 CAN-R9(IHC)-56-2 Wilm's Tumor AN584-5M, AN584-10M, AX584-YCD, NU584-UC 17, 27, 50, 135, 191 CB11 c-erbB-2 (HER-2/ neu) AM134-5ME, AM13410ME, AX134-YCDE, MU134-UCE 13, 24, 45, 81 14, 25, 46, 89, 187 AE3 AM009-5M, AM009-10M, AX009-YCD, MU009-UC Cat. No. 11, 23, 43, 54, 184 A62020069P CD2 Antibody 11, 43, 52 AM059-5ME, AX059-YCDE, MU059-UCE AB75 Clone www.biogenex.com Antibody Clone Index Clone Antibody Cat. No. Page CCP58 Mucin 2 (MUC2) AM358-5M, AX358-YCD, MU358-UC 15, 48, 113 CD20/C23 CD20 AM537-5M, AM537-10M, AX537-YCD, MU537-UC 12, 24, 44, 67, 185 AM549-5M, AM549-10M, AX549-YCD, MU549-UC 13, 24, 45, 76, 186 AM392-5M, AM392-10M, AX392-YCD, MU392A-UC 13, 24, 45, 81, 186 Carcinoembryonic Antigen (CEA) AM365-5M, AM365-10M, AX365-YCD, MU365-UC 13, 23, 43, 61, 185 Collagen IV AM379-5M, AM379-10M, AX379-YCD, MU379-UC 14, 46, 83 AM135-5M, AM135-10M, AX135-YCD 11, 23, 43, 58, 184 CD68/G2 CDX2-88 CEA88 COL-94 CU18 D33 CD68 CDX-2 Breast Cancer Antigen BCA 225 Desmin AM072-5M, AM072-10M, AX072-YCD, MU072-UC Thyroxine AM034A-5M, AX034-YCD, MU034A-UC 17, 50, 131, 191 DC-10 Cytokeratin 18 AM143-5M, AX143-YCD, MU143-UC 14, 46, 88, 187 DCS72 p27 (Kip1) AM396-5M, AX396-YCD, MU396-UC 16, 49, 119 DEK-10 Cytokeratin 10 AM201-5M, AX201-YCD, MU201-UC 14, 46, 87, 187 AM310-5M, AX310-YCD, MU310-UC 12, 45, 72, 186 DF1485 DFT-1 CD43 (T Cell, Leukosialin) AM305-5M, AX305-YCD, MU305-UC 12, 44, 72, 186 DM-1A Alpha-Tubulin MU121-UC 43, 53, 184 DM-1B Beta-Tubulin MU122-UC 43, 56, 184 DO7 p53 Protein AM239-5M, AM239-10M, AX239-YCD, MU239-UC 16, 27, 49, 121, 190 Dys1 (Dy4/6D3) Dystrophin AM243-5M, AX243-YCD 14, 46, 92, 187 Dystrophin AM244-5M, AX244-YCD 14, 47, 92, 187 Ep-CAM AN489-5M, AN489-10M, AX489-YCD, NU489-UC E17 Bcl-2 AN541-5M, AN541-10M, AX541-YCD, NU541-UC 11, 23,43, 54, 184 E18 Bcl-x AN462-5M, AN462-10M, AX462-YCD, NU462-UC 11, 23, 43, 55, 184 Dys2 (Dy8/6C5) E144 internationalcs@biogenex.com Antibody Cat. No. Page E247 Beta-Catenin AN510-5M, AN510-10M, AX510-YCD, NU510-UC 11, 23, 43, 56 ,184 E27 Cytokeratin 17 AM572-5M, AM572-10M, AX572-YCD, MU572-UC 14, 25, 46, 88, 187 E289 Stat 5-Alpha (C-term) AN480-5M, AN480-10M, AX480-YCD, NU480-UC 17, 27, 50, 128,191 E29 Epithelial Membrane Antigen (EMA) AM057-5M, AM057-10M, AX057-YCD, MU057-UC 14, 25, 47, 94, 187 E89 Caldesmon AN507-5M, AN507-10M, AX507-YCD, NU507-UC 11, 23, 43, 59, 184 E980.1 Factor XIII Subunit A AM337-5M, AM337-10M, AX337-YCD, MU337-UC 14, 25, 47, 97, 188 EP1 Estrogen Receptor-Alpha AN710-5ME, AN71010ME, AY710-YCDE, NU710-UCE 14, 25, 47, 96 EP1039Y NGF Receptor AN522-5M, AN522-10M, AX522-YCD, NU522-UC 16, 26, 49, 117, 190 EP1045Y ErbB-2/HER-2 AN471-5ME, AN471-10ME, AX471-YCDE 13, 24, 47, 95 EP1102Y Topo 2 Alpha AN576-5M, AN576-10M, AX576-YCD, NU576-UC 17, 27, 50, 132, 191 EP1150Y CD8 (C-Term) AN513-5M, AN513-10M, AX513-YCD, NU513-UC 12, 23, 44, 65, 185 EP125 Insulin AN735-5M, AN735-10M, AY735-YCD, NU735-UC 15, 26, 48, 106, 189 EP1255Y MMP-9 AN504-5M, AN504-10M, AX504-YCD, NU504-UC 15, 26, 48, 111, 189 EP1347Y CD11c AN555-5M, AN555-10M, AX555-YCD, NU555-UC 12, 24, 44, 66, 185 EP1373Y Apolipoprotein E AN567-5M, AN567-10M, AX567-YCD, NU567-UC 11, 23, 43, 53, 184 EP143 Oct 4 AN724-5M, AN724-10M, AY724-YCD, NU724-UC 16, 28, 49, 118, 190 EP148 CD48 AN721-5ME, AN72110ME, AY721-YCDE, NU721-UCE 13, 24, 45, 74 EP1582Y GCDFP-15 AN481-5M, AN481-10M, AX481-YCD, NU481-UC 14, 25, 47, 98, 188 14, 28, 46, 92,187 D5 CD44 (Phagocytic Glycoprotein1,HCAM) Clone 14, 25, 47, 94, 188 221 Antibody Clone Index Clone Antibody Cat. No. Page EP1615Y Cytokeratin 16 AN486-5M, AN486-10M, AX486-YCD, NU486-UC 14, 25, 46, 88 EP169 CD19 AN729-5M, AN729-10M, AY729-YCD, NU729-UC 12, 24, 44, 67, 185 Lambda Light Chain AN715-5M, AN715-10M, AY715-YCD, NU715-UC 15, 26, 48, 108, 189 EP176 CD205 AN737-5ME, AN73710ME, AY737-YCDE, NU737-UCE 13, 24, 45, 80 EP178 CD41/Integrin Alpha IIb AN732-5ME, AN73210ME, AY732-YCDE, NU732-UCE CD53 AN734-5M, AN734-10M, AY734-YCD, NU734-UC 13, 24, 45, 74, EP186 S100P AN712-5M, AN712-10M, AY712-YCD, NU712-UC 17, 27, 50, 127, 190 EP192 PMSA AN714-5M, AN714-10M, AY714-YCD, NU714-UC 17, 27, 50, 126, 190 EP2 Progesterone Receptor AN711-5ME, AN71110ME, AY711-YCDE, NU711-UCE 16, 27, 49, 124 CD4 AN722-5M, AN722-10M, AY722-YCD, NU722-UC 12, 23, 44, 63, 185 CD103 AN739-5ME, AN73910ME, AY739-YCDE, NU739-UCE 13, 24, 45, 79 CD63 AN720-5ME, AN72010ME, AY720-YCDE, NU720-UCE Claudin-5 AN718-5M, AN718-10M, AY718-YCD, NU718-UC Cytokeratin 5 & Cytokeratin 14 AN730-5ME, AN73010ME, AY730-YCDE, NU730-UCE 13, 25, 46, 85 AN482-5M, AN482-10M, AX482-YCD, NU482-UC 13, 24, 45, 75, 186 AN503-5M, AN503-10M, AX503-YCD, NU503-UC 17, 27, 50, 129, 191 AN512-5M, AN512-10M, AX512-YCD, NU512-UC 12, 23, 44, 64, 185 EP172 EP179 EP204 EP206 EP211 EP224 EP24 + EP61 EP2417Y EP2880Y EP2952 222 CD61 Survivin CD5 12, 24, 44, 71 Clone Antibody Cat. No. Page EP3 c-erbB-2 (HER-2/ neu) AN726-5ME, AN72610ME, AY726-YCDE, NU726-UCE 13, 24, 45, 81 EP3093 CD21 AN497-5M, AN497-10M, AX497-YCD, NU497-UC 12, 24, 44, 68, 185 EP31 NGFR AN738-5M, AN738-10M, AY738-YCD, NU738-UC 16, 26, 49, 117, 190 EP32 S100 Beta AN713-5M, AN713-10M, AY713-YCD, NU713-UC 17, 27, 50, 127, 190 EP3372 Factor XIII A AN516-5M, AN516-10M, AX516-YCD, NU516-UC 14, 25, 47, 97, 188 EP36 Bcl-2 AN723-5M, AN723-10M, AY723-YCD, NU723-UC 11, 23, 43, 55, 184 EP38Y EGFR(PAN) AN473-5ME, AN47310ME, AX473-YCDE, NU473-UCE 14, 25, 47, 93 EP4 Cytokeratin 4 AN717-5M, AN717-10M, AY717-YCD, NU717-UC 13, 25, 46, 84, 187 EP449E CD3 Epsilon AN477-10M 12, 23, 44, 62, 185 EP454Y Cyclin E2 AN519-5M, AN519-10M, AX519-YCD, NU519-UC 13, 25, 46, 84, 187 EP5 Ki-67 AN727-5M, AN727-10M, AY727-YCD, NU727-UC 14, 25, 48, 95, 188 EP54 CD146 AN716-5M, AN716-10M, AY716-YCD, NU716-UC 13, 24, 45, 80, 186 EP56 CD90 AN733-5M, AN733-10M, AY733-YCD, NU733-UC 13, 24, 45, 78, 186 EP6 E-Cadherin AN725-5M, AN725-10M, AY725-YCD, NU725-UC 14, 25, 47, 93, 187 EP798Y Calponin-1 (C-term) AN502-5M, AN502-10M, AX502-YCD, NU502-UC 11, 23, 43, 60, 184 EP82 CD79a AN719-5M, AN719-10M, AY719-YCD, NU719-UC 13, 24, 45, 77, 186 EP9 p53 AN728-5M, AN728-10M, AY728-YCD, NU728-UC 16, 26, 49, 120, 190 EPR1023 Mucin 1 (MUC1) AN564-5M, AN564-10M, AX564-YCD, NU564-UC 15, 26, 48, 112, 189 13, 24, 45, 75, 186 13, 24, 45, 82, 187 www.biogenex.com Antibody Clone Index Clone Antibody Cat. No. Page EPR1344 CD11b/ITAM AN546-5M, AN546-10M, AX546-YCD, NU546-UC 12, 24, 44, 66, 185 EPR1600Y Cytokeratin 5 AN484-5M, AN484-10M, AX484-YCD, NU484-UC 13, 25, 46, 85 AN588-5M, AN588-10M, AX588-YCD, NU588-UC 13, 25, 46, 85, 187 AN500-5M, AN500-10M, AX500-YCD, NU500-UC 13, 25, 46, 85, 187 AN485-5M, AN485-10M, AX485-YCD, NU485-UC 14, 25, 46, 88 AN528-5M, AN528-10M, AX528-YCD, NU528-UC 14, 25, 46, 86, 187 AN557-5M, AN557-10M, AX557-YCD, NU557-UC 14, 25, 46, 89, 187 16, 26, 49, 116, 189 EPR1600Y + EPR1602Y Cytokeratin 5 & 6 EPR1602Y Cytokeratin 6 EPR1614Y EPR1619Y EPR1622Y Cytokeratin 15 Cytokeratin 7 Cytokeratin 20 EPR1792Y N-Cadherin AN565-5M, AN565-10M, AX565-YCD, NU565-UC EPR2241(IHC)-32 Cyclin D1 AN474-5M, AN474-10M, AX474-YCD, NU474-UC 13, 24, 46, 84, 187 EPR3097Y CD99 AN518-5M, AN518-10M, AX518-YCD, NU518-UC 13, 24, 45, 78, 186 TFPU.1 AN520-5M, AN520-10M, AX520-YCD, NU520-UC PDCD4 AN524-5M, AN524-10M, AX524-YCD, NU524-UC 16, 27, 49, 122, 190 AN527-5M, AN527-10M, AX527-YCD, NU527-UC 15, 26, 48, 107, 189 AN515-5M, AN515-10M, AX515-YCD, NU515-UC 12, 24, 44, 65, 185 EPR3159Y EPR3432 EPR3611 EPR3653 Ki-67 CD14 PMS2 AN577-5M, AN577-10M, AX577-YCD, NU577-UC 16, 27, 49, 124, 190 EPR542 Oct 2 AN558-5M, AN558-10M, AX558-YCD, NU558-UC 16, 26, 49, 117, 190 EPR703(2) Estrogen Receptor (ER Alpha) AN509-5ME, AN50910ME, AX509-YCDE, NU509-UCE 14, 25, 47, 96 Estrogen Receptor (InSite® ER) AM368-5ME, AM36810ME, AX368-YCDE, MU368-UCE internationalcs@biogenex.com Antibody Cat. No. Page ErPr8 Prostate Specific Antigen(PSA) AM014-5ME, AM01410ME, AX014-YCDE, MU014-UCE 16, 27, 50, 126, 190 ES05 MLH1 AM703-5M, AM703-10M, AY703-YCD, MU703-UC 15, 26, 48, 111, 189 F14 Fibronectin AN569-5M, AN569-10M, AX569-YCD, NU569-UC 14, 25, 47, 98, 188 F39.4.1 Androgen Receptor AM256-2ME, AM25610ME, AX256-YCDE, MU256-UCE 11, 23, 43, 53, 184 F8 2.2.9 Factor VIII-Related antigen AM016-5M, AM016-10M, AX016-YCD, MU016-UC 14, 25, 47, 97, 188 FCN01 Fascin AM488-5M, AM488-10M, AX488-YCD, MU488-UC 14, 25, 47, 97, 188 FPC1 CD22 AM439-5M, AX439-YCD, MU439-UC 12, 44, 69, 185 G175-405 p16 (INK4a) AM540-5M, AM540-10M, MU540-UC 26, 49, 118, 190 G3.1 Heat Shock Protein 27 (HSP 27) AM171-5M, AM171-10M, AX171-YCD, MU171-UC 13, 26, 47, 101, 188 GA-5 Glial Fibrillary Acidic Protein (GFAP) AM020-10M 14, 25, 47, 99, 188 gp100/D5 Melanoma gp100 AM536-5M, AM536-10M, AX536-YCD, MU536-UC 15, 26, 48, 110, 189 GPC3-88 Glypican-3 (GPC3) AM539-5M, AM539-10M, MU539-UC 25, 47, 100, 188 H68.4 CD71 (Transferrin Receptor) AM354-5M, AX354-YCD, MU354-UC 13, 45, 77 HB125 Insulin AM029-5M, AW029-YCD, AX029-YCD, MU029-UC 15, 48, 105, 189 h-CD Caldesmon, High MW, Smooth muscle AM332-5M, AX332-YCD, MU332-UC 11, 43, 59 HHF35 Actin, MuscleSpecific AM090-5M, AM090-10M, AX090-YCD, MU090-UC 11, 23, 43, 51, 184 HMB45 Melanoma AM001-5M, AM001-10M, AX001-YCD, MU001A-UC 15, 26, 48, 109, 189 HO36.1.1 CD99 (MIC2 Antigen) AM355-5M, AM355-10M, AX355-YCD, MU355-UC 13, 24, 45, 78 HRS-4 CD30 (Ki-1 Antigen) AM351-5M, AM351-10M, AX351-YCD, MU351-UC 12, 24, 44, 70 17, 27, 52, 130, 191 EPR3947 ER88 Clone 14, 25, 47, 96, 188 223 Antibody Clone Index Clone Antibody Cat. No. Page Clone Antibody Cat. No. Page HSA/E8 HSA AM550-5M, AM550-10M, AX550-YCD, MU550-UC 15, 25, 48, 103, 188 LL002 Cytokeratin 14 AM146-5M, AM146-10M, AX146-YCD, MU146-UC 14, 25, 46, 87, 187 HT1/13.6.3 Transferrin AM025-5M, AX025-YCD 17, 50, 132, 191 Cytokeratin cocktail, Broad Spectrum Collagen III 13, 48, 83, 187 AM372-5M, AM372-10M, AX372-YCD, MU372-UC 14, 25, 46, 90, 187 AM167-5M, AX167-YCD, MU167-UC LL002+DEK10+RCK108+OVTL12/30+C11 LN1 CDw75 (B Cell) AM152-5M, AX152-YCD, MU152-UC 13, 45, 80, 186 Villin AM525-5M, AM525-10M, AX525-YCD, MU525-UC 17, 27, 50, 134, 191 LN2 CD74 (B Cell) 13, 45, 77, 186 AM367-5M, AX367-YCD, MU367-UC AM153-5M, AX153-YCD, MU153-UC 15, 48, 104, 188 LN22 Bcl-6 11, 23, 43, 55 IgM AM366-5M, AX366-YCD, MU366-UC 15, 48, 104, 188 AM708-5M, AM708-10M, AY708-YCD, MU708-UC LN3 HLA-DR AM154-5ME, AX154-YCDE, MU154-UCE 15, 47, 103, 188 Napsin A AM701-5M, AM701-10M, AY701-YCD, MU701-UC 16, 26, 49, 116, 189 LN5 Macrophage AM165-5M, AX165-YCD, MU165-UC 15, 48, 109, 189 14, 25, 46, 89, 187 LN6 Vimentin,NonHematopoietic AM163-5M, AX163-YCD, MU163-UC 17, 50, 134, 191 LO26 Myf4 AM432-5M, AM432-10M, AX432-YCD, MU432-UC 16, 26, 49, 114, 189 LP15 CD7 AM702-5M, AM702-10M, AY702-YCD, MU702-UC 12, 23, 44, 64, 185 Lu-5 Cytokeratin, Pan AM181-5M, AX181-YCD, MU181-UC 14, 46, 91, 187 M01 CD11b (C3bi Receptor) AM270-5M, AX270-YCD 12, 44, 66 M94138 Human Chorionic Gonadotropin (hCG) Beta AM395-5M, AX395-YCD, MU395-UC 15, 48, 103 MB1 CD45RA (B Cell) AM157-5M, AM157-10M, AX157-YCD 12, 24, 45, 73, 186 MB2 B Cell AM158-5M, AM158-10M, AX158-YCD, MU158-UC 11, 43, 54, 184 MBP88 Myelin Basic Protein AM380-5M, AM380-10M, AX380-YCD 16, 26, 48, 113 Mc5 Epithelial Membrane Antigen (EMA) AM182-5M, AM182-10M, AX182-YCD, MU182-UC 14, 25, 47, 94, 187 MDR88 Multi-Drug Resistance Marker (P-Glycoprotein) AM391-5M, AX391-YCD, MU391-UC 15, 48, 113 HWD1.1 ID2C3 IgG88 IgM88 IP64 IgG IT-Ks20.8 Cytokeratin 20 AM315-5M, AM315-10M, MU315-UC, AX315-YCD JB1a CD29 AM298-5M, AX298-YCD, MU298-UC 12, 44, 69, 185 JC/70A CD31 AM232-5M, AM232-10M, AX232-YCD 12, 24, 44, 70, 185 15, 48, 106 JC88 J Chain AM374-5M, AX374-YCD, MU374-UC JDR3B8 Beta-Tubulin II MU176-UC 43, 56, 184 JLN20 Alpha-Actinin AM097-5M, AX097-YCD, MU097-UC 11, 43, 52, 184 Ki-67 AM410-5M, AM410-10M, AX410-YCD, MU410-UC Kappa Light Chain AM369-5M, AM369-10M, AX369-YCD, MU369-UC 15, 26, 48, 106, 189 AM370-5M, AM370-10M, AX370-YCD, MU370-UC 15, 26, 48, 107, 189 AM416-5M, AM416-10M, AX416-YCD, MU416-UC 13, 24, 45, 76, 186 K-2 K88 Ki88 KP1 Ki-67 Antigen, Proliferating Cell CD68 15, 26, 48, 106, 189 L1C1 Kappa Light Chain AM048-5M, AM048-10M, AX048-YCD, MU048-UC 15, 26, 48, 106, 189 L-26 CD20 (B Cell) AM238-5M, AM238-10M, AX238-YCD, MU238A-UC 12, 24, 44, 185 ME.121 Prolactin AM031-5M, AM031-10M, AX031-YCD, MU031-UC 16, 28, 49, 125 LJ27.9 CD45 (Leukocyte common Antigen,LCA) AM338-5M, AX338-YCD, MU338-UC 12, 45, 73 MEM55 CD45RB AM320-5M, AX320-YCD, MU320-UC 12, 45, 73, 186 LK2H10 Chromogranin A AM126-5M, AM126-10M, AX126-YCD, MU126-UC 13, 24, 45, 82, 186 MEM55+LJ27.9 CD45 Cocktail (Leukocyte Antigen, LCA) AM371-5M, AM371-10M, AX371-YCD, MU371-UC 12, 24, 45, 73, 186 224 www.biogenex.com Antibody Clone Index Clone Antibody Cat. No. Page Clone Antibody Cat. No. Page MG-1 Myoglobin AM012-5M, AX012-YCD, MU012-UC 16, 49, 115, 189 NK-1 CD57 (Natural Killer Cell) AM314-5M, AX314-YCD, MU314-UC 13, 45, 75, 186 MIB-1 Ki-67 Antigen, Proliferating Cell AM297-5M, AM297-10M, AX297-YCD, MU297-UC 15, 26, 48, 107, 189 NKH-1 CD56 (Natural Killer Cell,NCAM) AM268-5M, AX268-YCD, MU268-UC 13, 45, 75 Neuron Specific Enolase (NSE) AM055-5M, AM055-10M, AX055-YCD, MU055-UC NKI/C3 MelanomaAssociated Antigen AM077-5M, AM077-10M, AX077-YCD, MU077-UC 15, 26, 48, 110, 189 O10 CD1a AM490-5M, AM490-10M, AX490-YCD, MU490-UC 11, 23, 44, 62, 185 MiTF AM554-5M, AM554-10M, AX554-YCD, MU554-UC ONS1A6 Beta-Tubulin IV MU178-UC 43, 57, 184 MOC-31 Epithelial-Specific Antigen AM316-5M, AM316-10M, MU316-UC, AX316-YCD OST1 Osteonectin AM387-5M, AX387-YCD, MU387-UC 16, 49, 118 Mouse 4A4 + Mouse34BE12 + Rabbit13H4 PIN4 (p63+CKHMW+ P504S) AM448-5ME, AM44810ME, AX448-YCDE, MU448-UCE Ov185:1 CA125 AM429-5M, AM429-10M, AX429-YCD, MU429-UC 11, 23, 43, 59, 184 Mouse 4A4 + Rabbit EPR1600Y OV-TL12/30 Cytokeratin 7 16, 27, 147 AM255-5M, AM255-10M, AX255-YCD, MU255-UC 14, 25, 46, 86, 187 p63 + CK5 AC602-5M, AC602-10M, AC602-YCD Mouse 4B12 + Rabbit EP1150Y CD4 + C8 Cocktail AC595-5M, AC595-10M, AC595-YCD 12, 23, 145 OV-TL12/30 & C51 Cytokeratin 7&8 AM587-5M, AM587-10M, AX587-YCD, MU587-UC 14, 25, 46, 86, 187 Mouse 56C6 + Rabbit EPR2241 (IHC)-32 CD10 and Cyclin D1 cocktail AC561-5M, AC561-10M, AC561-YCD 12, 24, 145, 185 PC10 AM252-5M, AM252-10M, AX252-YCD, MU252-UC 16, 27, 49, 125, 190 Mouse 7C7-D4 + Rabbit EPR1619Y CDX-2+CK7 cocktail AC5965M,AC59610M, AC596YCD Proliferating Cell Nuclear Antigen (PCNA) 13, 24, 145, 186 PD7/26/16 & 2B11 CD45 AM111-5M, AM111-10M, AX111-YCD 12, 24, 45, 72, 186 Mouse 7C7-D4 + Rabbit EPR1622Y+ EPR1619Y CDX-2+CK20+CK7 cocktail AC597-5M AC597-10M, AC597-YCD 13, 24, 147 PDGF88 Platelet-Derived Growth Factor (PDGF) AM376-5M, AX376-YCD, MU376-UC 16, 49, 123 Mouse 8G7G3/1 + Rabbit EP1582Y TTF-1+GCDFP-15 cocktail AC604-5M, AC604-10M, AC604-YCD 17, 27, 146, 191 PHE-5 Chromogranin A AM356-5M, AM356-10M, AX356-YCD, MU356-UC 13, 24, 45, 82, 187 Mouse 8G7G3/1 + Rabbit EP1582Y + EP1619Y TTF-1+CK20+CK7 cocktail AC605-5M, AC605-10M, AC605-YCD 17, 27, 147 PL8-F6 TTF1 and Cytokeratin 5/6 cocktail AC563-5M, AC563-10M, AC563-YCD AM228-5M, AM228-10M, AX228-YCD, MU228-UC 16, 27, 49, 123, 190 Mouse 8G7G3/1 + Rabbit EPR1600Y + Rabbit1602Y Placental Alkaline Phosphatase (PLAP) 17, 27, 146, 191 POH-1 p34cdc2 (Cyclin Dependent Kinase) AM301-5M, AX301-YCD, MU301-UC 16, 49, 119, 190 Mouse G175405 + Rabbit EPR3611 p16+Ki67 cocktail AC601-5M, AC601-10M 26, 146, 190 Polyclonal Alpha-1Antitrypsin AR015-5R, AW015-YCD, PU015-UP 11, 43, 52, 184 Mouse K-2 + Rabbit Poly Ki-67 and Lambda Light Chain Cocktail AC562-5M, AC562-10M, AC562-YCD 15, 26, 146, 189 Polyclonal Bax Protein AR347-5R, AW347-YCD, PU347-UP 11, 43, 54 Mouse L26 + Rabbit EP449E CD3 and CD20 cocktail AC559-5M, AC559-10M, AC559-YCD 12, 23, 145, 185 Polyclonal BRCA1 Protein AR345-5R, AW345-YCD, PU345-UP 11, 43, 55 MT1 & MB1 CD43 & CD45RA Cocktail AM159-5M, AX159-YCD 12, 44, 72, 186 Calretinin 11, 23, 60, 184 CD45RC (T Cell) AM156-5M, AX156-YCD, MU156-UC Polyclonal MT2 12, 45, 74, 186 AR4135R,AR41310R, AW413-YCD, PU413-UP MY-32 Myosin,Skeletal Muscle AM109-5M, AX109-YCD, MU109-UC 16, 49, 115, 189 Polyclonal CD23 12, 24, 44, 69, 185 NE-14 Neurofilament AM073-5M, AM073-10M, AX073-YCD, MU073-UC AR460-5R, AR460-10R, AW460-YCD, PU460-UP 16, 26, 49, 116, 190 Polyclonal Carcinoembryonic Antigen (CEA) AR009-5R, AW009-YCD 11, 43, 61, 185 MIG-N3 MiTF/A13 internationalcs@biogenex.com 16, 26, 49, 116, 190 15, 26, 48, 110, 189 14, 25, 47, 94, 188 16, 27, 147,190 225 Antibody Clone Index Clone Antibody Cat. No. Page Polyclonal Cyclin D1 AR447-5R, AR447-10R, AW447-YCD, PU447-UP 13, 24, 46, 83, 187 Polyclonal EGFR AR335-5RE, AW335-YCDE, PU335-UPE 14, 47, 93 Estrogen Receptor-Beta (ER-β) AR385-5R, AR385-10R, AW385-YCD, PU385-UP Polyclonal Estradiol AR038-5R, AW038-YCD 14, 47, 95, 188 Polyclonal Follicle Stimulating Hormone (FSH) AR026-5R, AW026-YCD 14, 47 Polyclonal Clone Antibody Cat. No. Page Polyclonal Lysozyme AR0245R,AR02410R, AW024-YCD, PU024-UP 15, 26, 48, 108, 189 Polyclonal Myeloperoxidase (MPO) AR4965R,AR49610R, AW496-YCD, PU496-UP 16, 26, 49, 114, 189 Polyclonal Myoglobin AR0125R,AW012YCD, PU012-UP 16, 49, 115, 189 Polyclonal Platelet-Derived Growth Factor (PDGF) AR3765R,AW376YCD, PU376-UP 16, 49, 124 Polyclonal S-100 Protein AR058-5R, AR058-10R, AW058-YCD, PU058-UP 17, 27, 50, 127, 190 Polyclonal Secretin AR0675R,AW067YCD 17, 50, 128, 191 Polyclonal Substance P AR069-5R, AW069-YCD, PU069-UP 17, 50, 128, 191 Polyclonal Terminal Deoxynucleotidyl Transferase (TdT) AR373-5R, AW373-YCD 17, 50, 130, 191 14, 25, 47, 96 Polyclonal Gastrin AR019-5R, AW019-YCD, PU019-UP Polyclonal Glial Fibrillary Acidic Protein (GFAP) AR020-5R, AR020-10R, AW020-YCD, PU020-UP Glucagon AR039-5R, AW039-YCD, PU039-UP Glutathione S-Transferase Pi (GST Pi) AR2495R,AW249YCD, PU249-UP 14, 47, 100, 188 15, 26, 47, 101 Polyclonal Toxoplasma gondii AR125-5RE, PU125-UPE 50, 132 Polyclonal Thyroid Stimulating Hormone (TSH) AR033-5R, AW033-YCD 17, 50, 131 Polyclonal VEGF AR483-5R, AR483-10R, AW483-YCD, PU483-UP 17, 27, 50, 134, 191 Polyclonal VIP AR530-5R, AR530-10R, AW530-YCD, PU530-UP 17, 27, 50, 135, 191 Polyclonal Catenin Delta 1 (p120) AR706-5R, AR706-10R, AW706-YCD, PU706-UP 11, 23, 44, 61, 185 Polyclonal Claudin 1 AR704-5R, AR704-10R, AW704-YCD, PU704-UP 13, 24, 45, 82, 187 Polyclonal HGH AR707-5R, AR707-10R, AW707-YCD, PU707-UP 15, 26, 47, 101, 188 PR88 Progesterone Receptor (InSite®PR) AM328-5ME, AM32810ME, AX328-YCDE, MU328-UCE 16, 27, 49, 125, 190 PS1 CD3 (T Cell) AM322-5M, AM322-10M, AX322-YCD, MU322-UC 12, 23, 44, 63, 185 Polyclonal Polyclonal 14, 47, 98, 188 14, 47, 99, 188 14, 47, 99, 188 Polyclonal Helicobacter pylori AR442-5RE, AR442-10RE, AW442-YCDE, PU442-UPE Polyclonal Hemoglobin A AR021-5R, AW021-YCD 15, 47, 102, 188 Polyclonal Hepatitis B Virus Core Antigen (HBcAg) AR082-5RE, PU082-UPE 47, 102 Placental Lactogen (hPL) AR0405R,AR04010R, AW040-YCD, PU040-UP 16, 27, 49, 123, 190 Polyclonal Herpes Simplex Virus Type I (HSV I) AR0845RE,AW084YCDE, PU084-UPE 15, 47, 102 Polyclonal Herpes Simplex Virus Type II (HSV II) AR085-5RE, AW085-YCDE, PU085-UPE 15, 47, 102 IgA AR045-5R, AW045-YCD, PU045-UP 15, 48, 103 Polyclonal Polyclonal IgG AR050-5R, AW050-YCD, PU050-UP IgM AR4275R,AR42710R, AW427-YCD, PU427-UP Polyclonal Lambda Light Chain AR049-5R, AW049-YCD, PU049-UP 15, 48, 108, 189 Polyclonal Laminin AR078-5R, AR078-10R, AW078-YCD, PU078-UP 15, 26, 48, 108, 189 PS2.1 pS2 Estrogen Inducible Protein AM190-5M, AM190-10M, AX190-YCD, MU190-UC 17, 27, 50, 126, 190 Polyclonal lgD AR440-5R, PU440-UP, AW440-YCD 15, 48, 104, 188 QBend/10 CD34 (Endothelial Cell) AM236-5M, AM236-10M, AX236-YCD, MU236-UC 12, 24, 44, 71, 185 Polyclonal Polyclonal 226 15, 48, 104, 188 15, 48, 105, 188 www.biogenex.com Antibody Clone Index Clone Antibody Cat. No. Page Clone Antibody Cat. No. Page R1 Inhibin-Alpha AM446-5M, AM446-10M, AX446-YCD, MU446-UC 15, 26, 48, 105, 189 TdT88 Terminal Deoxynucleotidyl Transferase (TdT) AM373-5M, AM373-10M, AX373-YCD, MU373-UC 13, 27, 50, 130 RBT-AMACR P504S (AMACR) AN538-5M, AN538-10M, AX538-YCD, NU538-UC 16, 26, 49, 120, 190 TGF88 Transforming Growth Factor (TGF),Alpha AM377-5M, AX377-YCD, MU377-UC 17, 50, 133 RCC (Renal Cell Carcinoma) AM543-5M, AM543-10M, AX543-YCD, MU543-UC Ty/G5 Tyrosinase AM535-5M, AM535-10M, AX535-YCD, MU535-UC 17, 27, 50, 133, 191 UCHL-1 CD45RO (T Cell) AM113-5M, AM113-10M, AX113-YCD, MU113-UC 13, 24, 45, 74, 186 Cytokeratin 19 AM246-5M, AM246-10M, AX246-YCD, MU246-UC UCHT1 CD3 (T Cell) AM258-5M, AX258-YCD, MU258-UC 12, 44, 63 CD35 AM431-5M, AM431-10M, AX431-YCD, MU431-UC V9 Vimentin AM074-5M, AM074-10M, AX074-YCD, MU074-UC 17, 27, 50, 134, 191 VU-4H5 CD227 (MUCIN 1) AM534-5M, AM534-10M, AX534-YCD, MU534-UC 15, 26, 45, 80, 186 Y113 Paxillin (N-Term) AN521-5M, AN521-10M, AX521-YCD, NU521-UC 16, 27, 49, 122, 190 Y236 p27 (Kip1) AN492-5M, AN492-10M, AX492-YCD, NU492-UC 16, 26, 49, 119, 190 Y92 PDGF Receptor-ß AN463-5M, AN463-10M, AX463-YCD, NU463-UC 16, 27, 49, 122, 190 YE291 ZAP-70 AN466-5M, AN466-10M, AX466-YCD, NU466-UC 17, 27, 50, 135, 191 YE372 Catenin (p120) AN523-5M, AN523-10M, AX523-YCD, NU523-UC 16, 26, 44, 61, 185 YR145 CD117 AN465-5M, AN465-10M, AX465-YCD, NU465-UC 13, 24, 45, 79, 186 ZAP70-C3 ZAP-70 AM544-5M, AM544-10M, AX544-YCD, MU544-UC 17, 27, 50, 135, 191 ZMSA-5 Sarcomeric Actin AM511-5M, AM511-10M, AX511-YCD, MU511-UC 17, 27, 50, 128, 190 ZP007 PAX-5 AM457-5M, AM457-10M, AX457-YCD, MU457-UC 16, 27, 49, 122, 190 RCC-26 RCK108 RLB25 17, 27, 50, 126, 190 14, 25, 46, 89, 187 12, 24, 44, 71, 185 RTJ1/A2 c-erbB-3 (HER-3) AM319-5M, AX319-YCD, MU319-UC 13, 24, 45, 81 SDL3D10 Beta-Tubulin III MU177-UC 43, 56, 184 SMMS.1 Myosin Heavy Chains, Smooth Muscle AM331-5M, AM331-10M, AX331-YCD, MU331-UC 16, 26, 49, 115 AM363-5M, AM363-10M, AX363-YCD, MU363-UC 17, 27, 50, 129, 191 AN740-5M, AN740-10M, AY740-YCD, NU740-UC 12, 23, 44, 65 AN741-5M, AN741-10M, AY741-YCD, NU741-YCD 12, 24, 44, 71 AN743-5M, AN743-10M, AY743-YCD, NU743-UC 15, 26, 48, 111 AN731-5ME, AN73110ME, AY731-YCDE, NU731-UCE 11, 23, 43, 58 AM505-5M, AM505-10M, AX505-YCD, MU505-UC 14, 25, 47, 100, 188 AM423-5M, AM423-10M, AX423-YCD, MU423-UC 13, 24, 45, 79, 186 CD8 (T Cell,Suppressor/ Cytotoxic) AM261-5M, AX261-YCD, MU261-UC 12, 44, 65 T9 CD71 (Transferrin Receptor) AM269-5M, AX269-YCD 13, 45, 76 TAU-2 Tau AM412-5M, AM412-10M, AX412-YCD, MU412-UC 17, 27, 50, 129, 191 Tau-5 Tau AM459-5M, AM459-10M, AX459-YCD, MU459-UC 17, 27, 50, 129, 191 Snp88 SP16 SP191 SP46 SP92 SPM498 T595 T8 Synaptophysin CD8 CD35 MSH2 BOB1 GLUT-1 CD117 internationalcs@biogenex.com 227 Listing by Categories Categories Clone Page CD34 (Endothelial Cell) QBend/10(M) 12, 24, 44, 71, 185 Androgen Receptor Myeloperoxidase (MPO) Polyclonal(R) 16, 26, 49, 114, 189 Bax Protein Polyclonal(R) 11, 43, 54 Bcl-2 E17(R) 11, 23,43, 54, 184 ACUTE MYELOID LEUKEMIA Clone Page F39.4.1(M) 11, 23, 43, 53, 184 BREAST PANEL ADHESION MOLECULES Beta-Catenin Categories E247(R) 11, 23, 43, 56 ,184 Bcl-2 Oncoprotein Bcl-2/100(M) 11, 23, 43, 55, 184 E247(R) 11, 23, 43, 56, 184 CD11b/ITAM EPR1344(R) 12, 24, 44, 66, 185 Beta-Catenin CD22 FPC1(M) 12, 44, 69, 185 BRCA1 Protein Polyclonal(R) 11, 43, 55 CD31 (PECAM-1) 9G11(M) 12, 44, 70, 185 Breast Cancer Antigen BCA 225 CU18(M) 11, 23, 43, 58, 184 CD44 (Phagocytic Glycoprotein1,HCAM) DF1485(M) 12, 45, 72, 186 CA 19-9 C241:5:1:4(M) 11, 23, 43, 58, 184 Calponin CALP(M) 11, 43, 59, 184 CD61 EP2417Y(R) 13, 24, 45, 75, 186 Calponin-1 (C-term) EP798Y(R) 11, 23, 43, 60, 184 E-Cadherin 36(M) 14, 25, 47, 93 Catenin (p120) YE372(R) 16, 26, 44, 61, 185 E-Cadherin EP6(R) 14, 25, 47, 93, 187 Catenin Delta 1 (p120) Polyclonal(R) 11, 23, 44, 61, 185 Ep-CAM E144(R) 14, 25, 47, 94, 188 Cathepsin D C15(M) 11, 44, 62 N-Cadherin EPR1792Y(R) 16, 26, 49, 116, 189 CD44 (Phagocytic Glycoprotein1,HCAM) DF1485(M) 12, 45, 72, 186 ADRENAL TUMORS CD66 BY114(M) 13, 45, 76, 186 CD117 T595(M) 13, 24, 45, 79, 186 CD117 YR145(R) 13, 24, 45, 79, 186 CD227 (MUCIN 1) VU-4H5(M) 15, 26, 45, 80, 186 CDX-2+CK20+CK7 cocktail CDX2-88(M)+ EPR1622Y(R)+ EPR1619Y(R) 13, 24, 147 CDX-2+CK7 cocktail CDX288(M)+EPR1619Y( R) 13, 24, 145, 186 BLADDER c-erbB-2 (HER-2/neu) CB11(M) 13, 24, 45, 81 CD44 (Phagocytic Glycoprotein1,HCAM) DF1485(M) 12, 45, 72, 186 c-erbB-2 (HER-2/neu) EP3(R) 13, 24, 45, 81 c-erbB-3 (HER-3) RTJ1/A2(M) 13, 24, 45, 81 c-erbB-2 (HER-2/neu) EP3(R) 13, 24, 45, 81 Cytokeratin 5 EPR1600Y(R) 13, 25, 46, 85 Cytokeratin 20 IT-Ks20.8(M) 14, 25, 46, 89, 187 Cytokeratin, High MW (Basic) AE3(M) 14, 46, 90, 187 Cytokeratin 5 & 6 EPR1600Y & EPR1602Y(R) 13, 25, 46, 85, 187 Cytokeratin, Low MW AE1(M) 14, 25, 46, 91, 187 Cytokeratin 5 & Cytokeratin 14 EP24/EP61(R) 13, 25, 46, 85 OV-TL12/30 14, 25, 46, 86, 187 OV-TL12/30 & C51(M) 14, 25, 46, 86, 187 CD56 (Natural Killer Cell,NCAM) NKH-1(M) 13, 45, 75 Inhibin-Alpha R1(M) 15, 26, 48, 105, 189 Synaptophysin Snp88(M) 17, 27, 50, 129, 191 AMYLOID Kappa Light Chain L1C1(M) 15, 26, 48, 106, 189 Kappa Light Chain K88(M) 15, 26, 48, 106, 189 Lambda Light Chain Polyclonal(R) 15, 48, 108, 189 Lambda Light Chain EP172(R) 15, 26, 48, 108, 189 Ki-67 K-2(M) 15, 26, 48, 106, 189 Cytokeratin 7 Ki-67 Antigen, Proliferating Cell MIB-1(M) 15, 26, 48, 107, 189 Cytokeratin 7 & 8 Ki-67 Antigen, Proliferating Cell Ki88(M) 15, 26, 48, 107, 189 p53 EP9(R) 16, 26, 49, 120, 190 p53 Protein BP53-12-1(M) 16, 26, 49, 120, 190 p53 Protein DO7(M) 16, 27, 49, 121, 190 p53 Protein 1801(M) 16, 27, 49, 121, 190 MMP-9 EP1255Y(R) 15, 26, 48, 111, 189 Blood Group Antigen Lewis A 7LE(M) 43, 57, 184 Blood Group Antigen Lewis B 2-25LE(M) 43, 57, 184 14, 25, 46, 87, 187 Cytokeratin, High MW (Basic) AE3(M) 14, 46, 90, 187 EGFR Polyclonal(R) 14, 47, 93 EP38Y(R) 14, 25, 47, 93 EP6(R) 14, 25, 47, 93, 187 E-Cadherin 36(M) 14, 25, 47, 93 erbB-2/HER-2 EP1045Y(R) 13, 24, 47, 95 Estrogen Receptor 1D5(M) 14, 25, 47, 95, 188 Estrogen Receptor EP1(R) 14, 25, 47, 96 11, 23, 43, 53, 184 Estrogen Receptor (ER Alpha) EPR703(2)(R) 14, 25, 47, 96 Estrogen Receptor (InSite® ER) ER88(M) 14, 25, 47, 96, 188 Estrogen Receptor-Beta (ER-β) Polyclonal(R) 14, 25, 47, 96 Fascin FCN01(M) 14, 25, 47, 97, 188 GCDFP-15 EP1582Y(R) 14, 25, 47, 98, 188 Macrophage LN5(M) 15, 48, 109, 189 MMP-9 EP1255Y(R) 15, 26, 48, 111, 189 MUC4 1G8(M) 15, 26, 48, 112, 189 Epithelial Membrane Antigen (EMA) E29(M) 14, 25, 47, 94, 187 Epithelial Membrane Antigen (EMA) Mc5(M) 14, 25, 47, 94, 187 Follicle Stimulating Hormone (FSH) Polyclonal(R) Human Chorionic Gonadotropin (hCG) Beta M94138(M) 15, 48, 103 HGH Polyclonal(R) 15, 26, 47, 101, 188 228 14, 25, 46, 86, 187 LL002(M) E-Cadherin BRAIN PATHOLOGY EP1373Y(R) C-51(M) Cytokeratin 14 EGFR(PAN) BLOOD GROUP ANTIGEN Apolipoprotein E Cytokeratin 8 14, 47 www.biogenex.com Listing by Categories Categories Clone Page Categories Clone Page Mucin 1 (MUC1) EPR1023(R) 15, 26, 48, 112, 189 Cytokeratin 7 OV-TL12/30 14, 25, 46, 86, 187 Mucin 2 (MUC2) CCP58(M) 15, 48, 113 Cytokeratin 7 EPR1619Y(R) 14, 25, 46, 86, 187 Myosin Heavy Chains,Smooth Muscle SMMS.1(M) 16, 26, 49, 115 Cytokeratin 7 & 8 OV-TL12/30 & C51(M) 14, 25, 46, 86, 187 p53 EP9(R) 16, 26, 49, 120, 190 Cytokeratin 10 DEK-10(M) 14, 46, 87, 187 p53 Protein BP53-12-1(M) 16, 26, 49, 120, 190 Cytokeratin 13 AE8(M) 14, 46, 87, 187 p53 Protein DO7(M) 16, 27, 49, 121, 190 Cytokeratin 17 E27(M) 14, 25, 46, 88, 187 p53 Protein 1801(M) 16, 27, 49, 121, 190 Cytokeratin 19 RCK108(M) 14, 25, 46, 89, 187 p63 4A4(M) 16, 27, 49, 121, 190 Cytokeratin 20 IT-Ks20.8(M) 14, 25, 46, 89, 187 p63+CK5 Cocktail 4A4(M)+ EPR1600Y( R) 16, 27, 147 Cytokeratin 20 EPR1622Y(R) 14, 25, 46, 89, 187 Progesterone Receptor EP2(R) 16, 27, 49, 124 Herpes Simplex Virus Type I (HSV I) Polyclonal 15, 47, 102 Progesterone Receptor 1A6(M) 16, 27, 49, 124 Herpes Simplex Virus Type II (HSV II) Polyclonal(R) 15, 47, 102 Progesterone Receptor (InSite®PR) PR88(M) 16, 27, 49, 125, 190 Ki-67 EP5(R) 14, 25, 48, 95, 188 Topo 2 Alpha EP1102Y(R) 17, 27, 50, 132, 191 Ki-67 K-2(M) 15, 26, 48, 106, 189 TTF-1+CK20+CK7 cocktail 8G7G3/1(M) + EPR1622Y(R) + EPR1619Y(R) 17, 27, 147 TTF-1+GCDFP-15 cocktail 8G7G3/1(M) + EP1582Y(R) 17, 27, 146, 191 Tumor-Associated Glycoprotein (TAG-72) B72.3(M) Tumor-Associated Glycoprotein (TAG-90 BCA) B6.2(M) Ki-67 EPR3611(R) 15, 26, 48, 107, 189 Ki-67 Antigen, Proliferating Cell MIB-1(M) 15, 26, 48, 107, 189 Ki-67 Antigen, Proliferating Cell Ki88(M) 15, 26, 48, 107, 189 p16 (INK4a) G175-405(M) 26, 49, 118, 190 17, 27, 50, 133, 191 p16+Ki67 cocktail "G175-405(M) +EPR3611( R)" 26, 146, 190 17, 50, 133, 191 p63 4A4(M) 16, 27, 49, 121, 190 Papillomavirus Type 16 (HPV-16) Cam Vir-1 16, 49, 121 NGF Receptor EP1039Y(R) 16, 26, 49, 117, 190 NGF Receptor EP31(R) 16, 26, 49, 117, 190 Bcl-2 E17(R) 11, 23, 43, 54, 184 Bcl-2 Oncoprotein bcl-2/100(M) 11, 23, 43, 55, 184 Beta-Catenin E247(R) 11, 23, 43, 56, 184 CDX-2+CK20+CK7 cocktail CDX2-88(M)+ EPR1622Y(R)+ EPR1619Y(R) 13, 24, 147 CDX-2+CK7 cocktail CDX288(M)+EPR1619Y( R) 13, 24, 145, 186 CELL SURFACE MARKERS CNS TUMORS DOG1 1.1(M) 14, 25, 46, 92, 187 Glut-1 SPM498(M) 15, 25, 47, 100, 188 CELLULAR ANTIGENS COLON Alpha-1-Antitrypsin Polyclonal(R) 11, 43, 52, 184 Cyclin D1 Polyclonal(R) 13, 24, 46, 83, 187 Cyclin D1 EPR2241(IHC)32(R) 13, 24, 46, 84, 187 Cyclin E2 EP454Y(R) 13, 25, 46, 84, 187 Dystrophin Dys1(Dy4/6D3)(M) 14, 46, 92, 187 Dystrophin Dys2(Dy8/6C5)(M) 14, 47, 92, 187 Glomerular Epithelial Protein 1 (GLEPP-1) 5C11(M) 14, 47, 99 Mitochondrial Antigen 113-1(M) 48, 111 Fascin FCN01(M) 14, 25, 47, 97, 188 P504S (AMACR) 13H4(R) 16, 26, 49, 120, 190 RBT-AMACR(R) 16, 26, 49, 120, 190 Myelin Basic Protein MBP88(M) 16, 26, 48, 113 Myoglobin MG-1(M) 16, 49, 115, 189 P504S (AMACR) Myoglobin Polyclonal(R) 16, 49, 115, 189 COLORECTAL PANEL Myosin,Skeletal Muscle MY-32(M) 16, 49, 115, 189 CA19-9 C241:5:1:4(M) 11, 23, 43, 58, 184 p27 (Kip1) DCS72(M) 16, 49, 119 CA 125 (Ovarian Tumor Marker) Ov185:1(M) 11, 23, 43, 59, 184 p27 (Kip1) Y236(R) 16, 26, 49, 119, 190 Carcinoembryonic Antigen (CEA) B01-94-11M-P(M) 11, 23, 43, 60, 185 16, 27, 49, 122, 190 Carcinoembryonic Antigen (CEA) CEA88(M) 13, 23, 43, 61, 185 PAX-5 ZP007(M) PDGF Receptor-ß Y92(R) 16, 27, 49, 122, 190 Carcinoembryonic Antigen (CEA) Polyclonal(R) 11, 43, 61, 185 PMS2 EPR3947(R) 16, 27, 49, 124, 190 CD10 56C6(M) 12, 23, 44, 65, 185 Survivin EP2880Y(R) 17, 27, 50, 129, 191 CDX-2 CDX2-88(M) 13, 24, 45, 81, 186 17, 50, 132, 191 c-myc Protein 9E 10(M) 13. 45, 83, 187 Cytokeratin 7 OV-TL12/30 14, 25, 46, 86, 187 Cytokeratin 7 EPR1619Y(R) 14, 25, 46, 86, 187 Cytokeratin 7 & 8 OV-TL12/30 & C51(M) 14, 25, 46, 86, 187 Transferrin HT1/13.6.3(M) CERVICAL Carcinoembryonic Antigen (CEA) B01-94-11M-P(M) 11, 23, 43, 60, 185 Carcinoembryonic Antigen (CEA) CEA88(M) 13, 23, 43, 61, 185 Carcinoembryonic Antigen (CEA) Polyclonal(R) 11, 43, 61, 185 c-myc Protein 9E 10(M) 13. 45, 83, 187 internationalcs@biogenex.com Cytokeratin 19 RCK108(M) 14, 25, 46, 89, 187 Cytokeratin 20 IT-Ks20.8(M) 14, 25, 46, 89, 187 229 Listing by Categories Categories Clone Page Categories Cytokeratin 20 EPR1622Y(R) 14, 25, 46, 89, 187 ENZYMES Cytokeratin Cocktail AE1 and AE3(M) 14, 25, 46, 89, 187 Alpha-1-Antichymotrypsin EGFR Polyclonal(R) 14, 47, 93 Alpha-1-Antitrypsin Polyclonal(R) 11, 43, 52, 184 EGFR(PAN) EP38Y(R) 14, 25, 47, 93 Cathepsin D C15(M) 11, 44, 62 Glut-1 SPM498(M) 15, 25, 47, 100, 188 EPITHELIAL MARKERS MLH1 ES05(M) 15, 26, 48, 111, 189 CD34 (Endothelial Cell) QBend/10(M) 12, 24, 44, 71, 185 MMP-9 EP1255Y(R) 15, 26, 48, 111, 189 Cytokeratin 4 EP4(R) 13, 25, 46, 84, 187 MSH2 SP46(R) 15, 26, 48, 111 Cytokeratin 4 6B10(M) 13, 25, 46, 84, 187 MSH6 2D4B5(M) 15, 26, 48, 112 ,189 MUC5AC 45M1(M) 15, 26, 48, 112, 189 Mucin 1 (MUC1) EPR1023(R) 15, 26, 48, 112, 189 Mucin 2 (MUC2) CCP58(M) 15, 48, 113 p21/WAF1 4D10(M) 16, 49, 119, 190 p53 EP9(R) 16, 26, 49, 120, 190 p53 Protein BP53-12-1(M) 16, 26, 49, 120, 190 p53 Protein DO7(M) 16, 27, 49, 121, 190 p53 Protein 1801(M) 16, 27, 49, 121, 190 PMS2 Villin EPR3947(R) ID2C3(M) 16, 27, 49, 124, 190 17, 27, 50, 134, 191 CYTOTOXIC DRUG METABOLISM Glutathione S-Transferase Pi (GST Pi) Multi-Drug Resistance Marker (P-Glycoprotein) Polyclonal(R) 14, 47, 100, 188 MDR88(M) 15, 48, 113 C3(M) 11, 23, 43, 53, 184 EMBRYONAL CARCINOMA Alpha-Fetoprotein (AFP) ENDOCRINE PANEL Clone Page α1A88(M) 11, 43, 52 Cytokeratin 6 EPR1602Y(R) 13, 25, 46, 85, 187 Cytokeratin 8 & 18 5D3(M) 14, 25, 46, 87, 187 Cytokeratin 18 DC-10(M) 14, 46, 88, 187 Cytokeratin Cocktail AE1 and AE3(M) 14, 25, 46, 89, 187 Cytokeratin cocktail, Broad Spectrum 34ßE12/C51/ AE1(M) 14, 46, 90, 187 Cytokeratin cocktail, Broad Spectrum CK88(M) 14, 25, 46, 90, 187 Cytokeratin, High MW 34ßE12(M) 14, 25, 46, 90, 187 Cytokeratin, High MW (Basic) AE3(M) 14, 46, 90, 187 Cytokeratin, Low MW AE1(M) 14, 25, 46, 91, 187 Cytokeratin, Pan Lu-5(M) 14, 46, 91, 187 Cytokeratin, Pan C11(M) 14, 46, 91 Collagen IV COL-94(M) 14, 46, 83 Ep-CAM E144(R) 14, 25, 47, 94, 188 Epithelial-Specific Antigen MOC-31(M) 14, 25, 47, 94, 188 Glomerular Epithelial Protein 1 (GLEPP-1) 5C11(M) 14, 47, 99 MUC4 1G8(M) 15, 26, 48, 112, 189 Mucin 2 (MUC2) CCP58(M) 15, 48, 113 NKH-1(M) 13, 45, 75 13, 48, 83, 187 ACTH AH26(M) 11, 23, 43, 51, 184 Estradiol Polyclonal(R) 14, 47, 95, 188 Follicle Stimulating Hormone (FSH) Polyclonal(R) 14, 47 Glucagon Polyclonal(R) 14, 47, 99, 188 Collagen III HWD1.1(M) HGH Polyclonal(R) 15, 26, 47, 101, 188 Collagen IV COL-94(M) 14, 46, 83 Fibronectin F14(R) 14, 25, 47, 98, 188 Laminin Polyclonal(R) 15, 26, 48, 108, 189 Osteonectin OST1(M) 16, 49, 118 Nuclear Ribonucleoprotein 58-15(M) 49, 117, 190 Carcinoembryonic Antigen (CEA) B01-94-11M-P(M) 11, 23, 43, 60, 185 Carcinoembryonic Antigen (CEA) CEA88(M) 13, 23, 43, 61, 185 CD56 (Natural Killer Cell,NCAM) Human Chorionic Gonadotropin (hCG) Beta M94138(M) 15, 48, 103 Inhibin-Alpha R1(M) 15, 26, 48, 105, 189 Insulin EP125(R) 15, 26, 48, 106, 189 Insulin HB125(M) 15, 48, 105, 189 Prolactin ME.121(M) 16, 28, 49, 125 Thyroglobulin 2H11(M) 17, 27, 50, 130, 191 Thyroid Stimulating Hormone (TSH) 5404(M) 17, 27, 50, 131 Thyroid Stimulating Hormone (TSH) Polyclonal(R) 17, 50, 131 Thyroxine D5(M) 17, 50, 131, 191 V9(M) 17, 27, 50, 134, 191 ENDOMETRIOID CARCINOMA Vimentin EWING'S SARCOMA ENDOTHELIAL VASCULAR MARKER EXTRACELLULAR MATRIX PROTEIN GASTROINTESTINAL PANEL Carcinoembryonic Antigen (CEA) Polyclonal(R) 11, 43, 61, 185 CD117 T595(M) 13, 24, 45, 79, 186 CD117 YR145(R) 13, 45, 79, 186 CDX-2 CDX2-88(M) 13, 24, 45, 81, 186 c-erbB-2 (HER-2/neu) EP3(R) 13, 24, 45, 81 Cytokeratin 7 OV-TL12/30 14, 25, 46, 86, 187 Cytokeratin 17 E27(M) 14, 25, 46, 88, 187 CD31 (Endothelial Cell) JC/70A(M) 12, 24, 44, 70, 185 DOG1 1.1(M) 14, 25, 46, 92, 187 CD31 (PECAM-1) 9G11(M) 12, 44, 70, 185 Secretin Polyclonal(R) 17, 50, 128, 191 CD34 (Endothelial Cell) QBend/10(M) 12, 24, 44, 71, 185 Substance P Polyclonal(R) 17, 50, 128, 191 Factor VIII-Related antigen F8 2.2.9(M) 14, 25, 47, 97, 188 TGF88(M) 17, 50, 133 Factor XIII Subunit A E980.1(M) 14, 25, 47, 97, 188 Transforming Growth Factor (TGF),Alpha Factor XIII A EP3372(R) 14, 25, 47, 97, 188 230 www.biogenex.com Listing by Categories Categories Clone Page TTF-1+CK20+CK7 cocktail “8G7G3/1+ EPR1622Y+ EPR1619Y” 17, 27, 147 GERM CELL TUMORS Alpha-Fetoprotein (AFP) C3(M) 11, 23, 43, 53, 184 Glypican-3 (GPC3) GPC3-88(M) 25, 47, 100, 188 CD30 (Ki-1 Antigen) HRS-4(M) 12, 24, 44, 70 CD117 T595(M) 13, 24, 45, 79, 186 CD117 YR145(R) 13, 45, 79, 186 Human Chorionic Gonadotropin (hCG) Beta M94138(M) 15, 48, 103 Oct 4 EP143(R) Placental Alkaline Phosphatase (PLAP) PL8-F6(M) Categories Clone Page IgM IgM88(M) 15, 48, 104, 188 IgM Polyclonal(R) 15, 48, 105, 188 J Chain JC88(M) 15, 48, 106 Kappa Light Chain L1C1(M) 15, 26, 48, 106, 189 Kappa Light Chain K88(M) 15, 26, 48, 106, 189 Lambda Light Chain EP172(R) 15, 26, 48, 108, 189 Lambda Light Chain Polyclonal(R) 15, 48, 108, 189 INFECTIOUS AGENTS Adenovirus A62020069P(M) 11, 43, 52 Cytomegalovirus (CMV) BM204(M) 46, 91 16, 28, 49, 118, 190 Epstein-Barr Virus Early Antigen 1108-1(M) 47, 95, 188 16, 27, 49, 123, 190 Helicobacter pylori Polyclonal(R) 15, 26, 47, 101 Hepatitis B Virus Core Antigen (HBcAg) Polyclonal(R) 47, 102 GYNECOLOGICAL Placental Alkaline Phosphatase (PLAP) PL8-F6(M) 16, 27, 49, 123, 190 Herpes Simplex Virus Type I (HSV I) Polyclonal(R) 15, 47, 102 Placental Lactogen (hPL) Polyclonal(R) 16, 27, 49, 123, 190 Papillomavirus Type 16 (HPV-16) CamVir-1(M) 16, 49, 121 Toxoplasma gondii Polyclonal(R) 50, 132 CD31 (PECAM-1) 9G11(M) 12, 44, 70, 185 INTERMEDIATE FILAMENTS & CYTOSKELETAL PROTEINS CD34 (Endothelial Cell) QBend/10(M) 12, 24, 44, 71,185 HEMANGIOBLASTOMA Actin, Muscle-Specific HHF35(M) HEMATOPOIETIC / LYMPHOID MARKERS Actin, Smooth Muscle 1A4(M) 11, 23, 43, 51, 184 CD41/Integrin Alpha IIb EP178(R) 12, 24, 44, 71 Alpha-Actinin JLN20(M) 11, 43, 52, 184 CD53 EP179(R) 13, 24, 45, 74, Alpha-Tubulin DM-1A(M) 43, 53, 184 47, 100, 188 Beta-Tubulin DM-1B(M) 43, 56, 184 Granulocyte BM-2(M) 11, 23, 43, 51, 184 Hemoglobin A Polyclonal(R) 15, 47, 102, 188 Beta-Tubulin II JDR3B8(M) 43, 56, 184 IgA Polyclonal(R) 15, 48, 103 Beta-Tubulin III SDL3D10(M) 43, 56, 184 IgD Polyclonal(R) 15, 48, 104, 188 Beta-Tubulin IV ONS1A6(M) 43, 57, 184 15, 48, 104, 188 Caldesmon E89(R) 11, 23, 43, 59, 184 Caldesmon, High MW, Smooth muscle h-CD(M) 11, 43, 59 Calponin CALP(M) 11, 43, 59, 184 Calponin-1 (C-term) EP798Y(R) 11, 23, 43, 60, 184 IgG IgG88(R) IgG Polyclonal(R) 15, 48, 104, 188 IgM IgM88(M) 15, 48, 104, 188 IgM Polyclonal(R) 15, 48, 105, 188 Bcl-6 LN22 11, 23, 43, 55 BOB-1 SP92(R) 11, 23, 43, 58 CD15 (Blood group antigen Lewis X) BRA4F1(M) 12, 24, 44, 67, 185 CD30 (Ki-1 Antigen) Ber-H2(M) 12, 24, 44, 70, 185 CD30 (Ki-1 Antigen) HRS-4(M) 12, 24, 44, 70 Fascin FCN01(M) HODGKIN'S LYMPHOMA Desmin D33(M) 14, 28, 46, 92,187 Dystrophin Dys1(Dy4/6D3)(M) 14, 46, 92, 187 Dystrophin Dys2(Dy8/6C5)(M) 14, 47, 92, 187 Fascin FCN01(M) 14, 25, 47, 97, 188 Glial Fibrillary Acidic Protein (GFAP) GA-5(M) 14, 25, 47, 99, 188 14, 25, 47, 97, 188 Glial Fibrillary Acidic Protein (GFAP) Polyclonal(R) 14, 47, 99, 188 16, 48, 113 Oct 2 EPR542(R) 16, 26, 49, 117, 190 Muscle Actins Actin 88 Cocktail(M) TFPU.1 EPR3159Y(R) 17, 27, 52, 130, 191 Myf4 LO26(M) 16, 26, 49, 114, 189 Myoglobin MG-1(M) 16, 49, 115, 189 HORMONE REGULATED PROTEIN Cathepsin D C15(M) 11, 44, 62 Myoglobin Polyclonal(R) 16, 49, 115, 189 Gastrin Polyclonal(R) 14, 47, 98, 188 Myosin Heavy Chains, Smooth Muscle SMMS.1(M) 16, 26, 49, 115 Heat Shock Protein (HSP-70) BRM-22(M) 15, 47, 101, 188 Myosin, Skeletal Muscle MY-32(M) 16, 49, 115, 189 Heat Shock Protein 27 (HSP 27) G3.1(M) 13, 25, 47, 101, 188 Neurofilament NE-14(M) 16, 26, 49, 116, 190 pS2 Estrogen Inducible Protein PS2.1(M) 17, 27, 50, 126, 190 Osteonectin OST1(M) 16, 49, 118 Secretin Polyclonal(R) 17, 50, 128, 191 Paxillin (N-Term) Y113(R) 16, 27, 49, 122, 190 IMMUNOGLOBULIN & COMPLEMENT PROTEINS Sarcomeric Actin ZMSA-5(M) 17, 27, 50, 128, 190 IgA Polyclonal(R) 15, 48, 103 Tau Tau-2(M) 17, 27, 50, 129, 191 IgD Polyclonal(R) 15, 48, 104, 188 Tau Tau-5(M) 17, 27, 50, 129, 191 IgG IgG88(R) 15, 48, 104, 188 Villin ID2C3(M) 17, 27, 50, 134, 191 internationalcs@biogenex.com 231 Listing by Categories Categories Clone Page Categories Clone Vimentin V9(M) 17, 27, 50, 134, 191 EGFR Polyclonal(R) 14, 47, 93 Vimentin,Non-Hematopoietic LN6(M) 17, 50, 134, 191 EGFR(PAN) EP38Y(R) 14, 25, 47, 93 Epithelial Membrane Antigen (EMA) E29(M) 14, 25, 47, 94, 187 Epithelial Membrane Antigen (EMA) Mc5(M) 14, 25, 47, 94, 187 KIDNEY: RENAL EPITHELIAL TUMORS Page CD117 T595(M) 13, 24, 45, 79, 186 CD117 YR145(R) 13, 45, 79, 186 Epithelial-Specific Antigen MOC-31(M) 14, 25, 47, 94, 188 CD61 EP2417Y(R) 13, 24, 45, 75, 186 Fascin FCN01(M) 14, 25, 47, 97, 188 HLA-DR LN3(M) 15, 47, 103, 188 Ki-67 EP5(R) 14, 25, 48, 95, 188 K-2(M) 15, 26, 48, 106, 189 LEUKEMIA Lysozyme Polyclonal(R) 15, 26, 48, 108, 189 Ki-67 MMP-9 EP1255Y(R) 15, 26, 48, 111, 189 Ki-67 EPR3611(R) 15, 26, 48, 107, 189 Myeloid specific Antigen BM-3(M) 16, 48, 114, 189 BM-1(M) 16, 48, 114 K-2(M) and Polyclonal (R) 15, 26, 146, 189 Myeloid specific Antigen Ki-67 and Lambda Light Chain Cocktail Ki-67 Antigen,Proliferating Cell MIB-1(M) 15, 26, 48, 107, 189 Ki-67 Antigen,Proliferating Cell Ki88(M) 15, 26, 48, 107, 189 Mast Cell Tryptase AA1(M) 26, 48, 109, 189 Mesothelin 5B2(M) 15, 26, 48, 110, 189 Napsin A IP64(M) 16, 26, 49, 116, 189 LIVER Alpha-1-Antichymotrypsin α1A88(M) 11, 43, 52 Alpha-1-Antitrypsin Polyclonal(R) 11, 43, 52, 184 Alpha-Fetoprotein (AFP) C3(M) 11, 23, 43, 53, 184 Glypican-3 (GPC3) GPC3-88(M) 25, 47, 100, 188 HSA HSA/E8(M) 15, 25, 48, 103, 188 p53 EP9(R) 16, 26, 49, 120, 190 p53 Protein BP53-12-1(M) 16, 26, 49, 120, 190 p53 Protein DO7(M) 16, 27, 49, 121, 190 p53 Protein 1801(M) 16, 27, 49, 121, 190 Calretinin 2E7(M) 11, 23, 43, 60, 184 Calretinin Polyclonal(R) 11, 23, 60, 184 Carcinoembryonic Antigen (CEA) B01-94-11M-P(M) 11, 23, 43, 60, 185 Carcinoembryonic Antigen (CEA) CEA88(M) 13, 23, 43, 61, 185 Carcinoembryonic Antigen (CEA) Polyclonal(R) 11, 43, 61, 185 CD44 (Phagocytic Glycoprotein1,HCAM) DF1485(M) 12, 45, 72, 186 CD66 BY114(M) 13, 45, 76, 186 CDX-2+CK20+CK7 cocktail CDX2-88(M)+ EPR1622Y(R)+ EPR1619Y(R) 13, 24, 147 CDX-2+CK7 cocktail CDX288(M)+EPR1619Y( R) 13, 24, 145, 186 Chromogranin A LK2H10(M) 13, 24, 45, 82, 186 LYMPHOMA LUNG Neuron Specific Enolase (NSE) MIG-N3(M) 16, 26, 49, 116, 190 p21/WAF1 4D10(M) 16, 49, 119, 190 p53 EP9(R) 16, 26, 49, 120, 190 p53 Protein BP53-12-1(M) 16, 26, 49, 120, 190 p53 Protein DO7(M) 16, 27, 49, 121, 190 p53 Protein 1801(M) 16, 27, 49, 121, 190 S100P EP186(R) 17, 27, 50, 127, 190 Synaptophysin Snp88(M) 17, 27, 50, 129, 191 Thyroid Transcription Factor (TTF-1) 8G7G3/1(M) 17, 27, 50, 131, 191 TTF1 and Cytokeratin 5/6 cocktail 8G7G3/1(M) + EPR1600Y(R) + EPR1602Y(R) 17, 27, 146, 191 TTF-1+CK20+CK7 cocktail 8G7G3/1(M) + EPR1622Y(R) + EPR1619Y(R) 17, 27, 147 TTF-1+GCDFP-15 cocktail 8G7G3/1(M) + EP1582Y(R) 17, 27, 146, 191 VEGF Polyclonal(R) 17, 27, 50, 134, 191 NK-1(M) 13, 45, 75, 186 LYMPHOCYTE DISORDERS CD57 (Natural Killer Cell) Chromogranin A PHE-5(M) 13, 24, 45, 82, 187 14, 25, 47, 94, 187 EP224(R) 13, 24, 45, 82, 187 Epithelial Membrane Antigen (EMA) E29(M) Claudin-5 Cytokeratin 5 & 6 EPR1600Y & EPR1602Y(R) 13, 25, 46, 85, 187 Epithelial Membrane Antigen (EMA) Mc5(M) 14, 25, 47, 94, 187 Cytokeratin 7 OV-TL12/30 14, 25, 46, 86, 187 Ki-67 and Lambda Light Chain Cocktail K-2(M) and Polyclonal(R) 15, 26, 146, 189 Cytokeratin 7 EPR1619Y(R) 14, 25, 46, 86, 187 Macrophage LN5(M) 15, 48, 109, 189 Cytokeratin 7 & 8 OV-TL12/30 & C51(M) 14, 25, 46, 86, 187 p34cdc2 (Cyclin Dependent Kinase) POH-1(M) 16, 49, 119, 190 Cytokeratin 8 & 18 5D3(M) 14, 25, 46, 87, 187 Synaptophysin Snp88(M) 17, 27, 50, 129, 191 Cytokeratin 19 RCK108(M) 14, 25, 46, 89, 187 Cytokeratin 20 IT-Ks20.8(M) 14, 25, 46, 89, 187 Cytokeratin 20 EPR1622Y(R) 14, 25, 46, 89, 187 Cytokeratin Cocktail AE1 and AE3(M) 14, 25, 46, 89, 187 Cytokeratin, High MW 34ßE12(M) 14, 25, 46, 90, 187 Cytokeratin, High MW (Basic) AE3(M) 14, 46, 90, 187 Cytokeratin, Low MW AE1(M) 14, 25, 46, 91, 187 232 LYMPHOMA (B PANEL) B Cell MB2(M) 11, 43, 54, 184 B Lymphocyte Antigen 36 (BLA.36) A27-42(M) 11, 23, 43, 54, 184 Bcl-2 EP36(R) 11, 23, 43, 55, 184 BOB-1 SP92(R) 11, 23, 43, 58 CD3 and CD20 cocktail EP449E(R) and L-26(M) 12, 23, 145, 185 www.biogenex.com Listing by Categories Categories Clone Page Categories Clone Page CD19 EP169(R) 12, 24, 44, 67, 185 CD103 EP206(R) 13, 24, 45, 79 CD20 CD20/C23(M) 12, 24, 44, 67, 185 TIA-1 2G9A10F5(M) 17, 27, 50, 132 CD20 (B Cell) L-26(M) 12, 24, 44, 185 VIP Polyclonal(R) 17, 27, 50, 135, 191 CD20 (B Cell) B1(M) 12, 44, 68 LYMPHOMAS & LEUKEMIAS CD21 EP3093(R) 12, 24, 44, 68, 185 Bcl-2 E17(R) 11, 23, 43, 54, 184 CD21(B Cell) B2(M) 12, 44, 68 Bcl-2 Oncoprotein Bcl-2/100(M) 11, 23, 43, 55, 184 CD22 FPC1(M) 12, 44, 69, 185 Bcl-6 LN22 11, 23, 43, 55 CD23 Polyclonal(R) 12, 24, 44, 69, 185 BOB-1 SP92(R) 11, 23, 43, 58 CD25 4C9(M) 12, 24, 44, 69, 185 CD10 56C6(M) 12, 23, 44, 65, 185 12, 45, 73 CD10 and Cyclin D1 cocktail 56C6(M) and EPR2241(R ) 12, 24, 145, 185 M01(M) 12, 44, 66 CD45 (Leukocyte common Antigen,LCA) LJ27.9(M) CD45RA (B Cell) MB1(M) 12, 24, 45, 73, 186 CD11b (C3bi Receptor) CD79a EP82(R) 13, 24, 45, 77, 186 CD11b/ITAM EPR1344(R) 12, 24, 44, 66, 185 CD79a 11E 3(M) 13, 24, 45, 77, 186 CD11C EP1347Y(R) 12, 24, 44, 66, 185 CDw75 (B Cell) LN1(M) 13, 45, 80, 186 CD14 EPR3653(R) 12, 24, 44, 65, 185 IgD Polyclonal(R) 15, 48, 104, 188 CD29 JB1a(M) 12, 44, 69, 185 IgM IgM88(M) 15, 48, 104, 188 CD35 SP191(R) 12, 24, 44, 71 IgM Polyclonal(R) 15, 48, 105, 188 CD35 RLB25(M) 12, 24, 44, 71, 185 Kappa Light Chain L1C1(M) 15, 26, 48, 106, 189 CD43 & CD45RA Cocktail MT1 &MB1(M) 12, 44, 72, 186 Kappa Light Chain K88(M) 15, 26, 48, 106, 189 EP172(R) 15, 26, 48, 108, 189 CD45 (Leukocyte common Antigen,LCA) PD7/26/16& 2B11(M) 12, 24, 45, 72, 186 Lambda Light Chain Lambda Light Chain Polyclonal(R) 15, 48, 108, 189 CD45 Cocktail (Leukocyte Antigen, LCA) MEM55+LJ27.9 (M) 12, 24, 45, 73, 186 Oct 2 EPR542(R) 16, 26, 49, 117, 190 CD45RB MEM55(M) 12, 45, 73, 186 PAX-5 ZP007(M) 16, 27, 49, 122, 190 CD48 EP148(R) 13, 24, 45, 74 CD68 KP1(M) 13, 24, 45, 76, 186 11, 23, 44, 62, 185 CD68 CD68/G2(M) 13, 24, 45, 76, 186 LYMPHOMA (T PANEL) CD1a O10(M) CD2 AB75(M) 11, 44, 62, 185 CD71 (Transferrin Receptor) T9(M) 13, 45, 76 CD3 Epsilon EP449E(R) 12, 23, 44, 62, 185 CD71 (Transferrin Receptor) H68.4(M) 13, 45, 77 CD3 (T Cell) UCHT1(M) 12, 44, 63 CD74 (B Cell) LN2(M) 13, 45, 77, 186 CD3 (T Cell) PS1(M) 12, 23, 44, 63, 185 CD90 EP56(R) 13, 24, 45, 78, 186 CD3 and CD20 cocktail EP449E(R) and L-26(M) 12, 23, 145, 185 CD105 4G11(M) 13, 45, 79, 186 CD117 T595(M) 13, 24, 45, 79, 186 CD117 YR145(R) 13, 45, 79, 186 CD205 EP176(R) 13, 24, 45, 80 Cyclin D1 Polyclonal(R) 13, 24, 46, 83, 187 Cyclin D1 EPR2241(IHC)32(R) 13, 24, 46, 84, 187 Oct 2 EPR542(R) 16, 26, 49, 117, 190 Terminal Deoxynucleotidyl Transferase (TdT) TdT88(M) 13, 27, 50, 130 17, 50, 130, 191 CD4 EP204(R) 12, 23, 44, 63, 185 CD4 4B12(M) 12, 23, 44, 63, 185 CD4 + C8 Cocktail 4B12(M)+ EP1150Y(R) 12, 23, 145 CD5 4C7(M) 12, 23, 44, 64, 185 CD5 EP2952(R) 12, 23, 44, 64, 185 CD7 LP15(M) 12, 23, 44, 64, 185 CD8 SP16(R) 12, 23, 44, 65 CD8 1A5(M) 12, 23, 44, 64, 185 CD8 (C-Term) EP1150Y(R) 12, 23, 44, 65, 185 Terminal Deoxynucleotidyl Transferase (TdT) Polyclonal(R) CD8 (T Cell,Suppressor/Cytotoxic) T8(M) 12, 44, 65 ZAP-70 ZAP70-C3(M) 17, 27, 50, 135, 191 CD16 2H7(M) 12, 44, 67, 185 ZAP-70 YE291(R) 17, 27, 50, 135, 191 CD25 4C9(M) 12, 24, 44, 69, 185 MELANOMA CD43 (T Cell, Leukosialin) DFT-1(M) 12, 44, 72, 186 CD63 EP211(R) 13, 24, 45, 75, 186 CD146 EP54(R) 13, 24, 45, 80, 186 CD45 (Leukocyte common Antigen,LCA) LJ27.9(M) 12, 45, 73 CD45RC (T Cell) MT2(M) 12, 45, 74, 186 CD45RO (T Cell) UCHL-1(M) 13, 24, 45, 74, 186 CD95 EP208(R) 13, 24, 45, 78 CD99 EPR3097Y(R) 13, 24, 45, 78, 186 CD99 (MIC2 Antigen) HO36.1.1(M) 13, 24, 45, 78 internationalcs@biogenex.com Melan-A (MART-1) A103(M) 15, 26, 48, 109, 189 Melanoma HMB45(M) 15, 26, 48, 109, 189 Melanoma gp100 gp100/D5(M) 15, 26, 48, 110, 189 Melanoma-Associated Antigen NKI/C3(M) 15, 26, 48, 110, 189 MiTF MiTF/A13 15, 26, 48, 110, 189 MMP-9 EP1255Y(R) 15, 26, 48, 111, 189 233 Listing by Categories Categories Clone Page Categories S100 beta EP32(R) 17, 27, 50, 127, 190 NEUROFIBROMA S100 Protein Polyclonal(R) 17, 27, 50, 127, 190 Claudin 1 S100 Protein 15E2E2(M) 17, 27, 50, 127, 190 NATURAL KILLER CELL PANEL Tyrosinase Ty/G5(M) 17, 27, 50, 133, 191 Polyclonal(R) 13, 24, 45, 82, 187 Alpha-Fetoprotein (AFP) C3(M) 11, 23, 43, 53, 184 E-Cadherin 36(M) 14, 25, 47, 93 Bcl-2 E17(R) 11, 23, 43, 54, 154 E-Cadherin EP6(R) 14, 25, 47, 93, 187 Bcl-2 Oncoprotein bcl-2/100(M) 11, 23, 43, 55, 184 CA19-9 C241:5:1:4(M) 11, 23, 43, 58, 184 MENINGIOMAS Claudin 1 MERKEL CELL CARCINOMA MESOTHELIOMA CA 125 (Ovarian Tumor Marker) Clone Page Polyclonal(R) 13, 24, 45, 82, 187 CD56 (Natural Killer Cell,NCAM) NKH-1(M) 13, 45, 75 CD57 (Natural Killer Cell) NK-1(M) 13, 45, 75, 186 OVARIAN MARKERS Ov185:1(M) 11, 23, 43, 59, 184 CA 125 (Ovarian Tumor Marker) Ov185:1(M) 11, 23, 43, 59, 184 Cytokeratin 5 EPR1600Y(R) 13, 25, 46, 85 CD44 (Phagocytic Glycoprotein1,HCAM) DF1485(M) 12, 45, 72, 186 Cytokeratin 5 & 6 EPR1600Y & EPR1602Y(R) 13, 25, 46, 85, 187 CDX-2 CDX2-88(M) 13, 24, 45, 81, 186 Mesothelin 5B2(M) 15, 26, 48, 110, 189 Cytokeratin 5 EPR1600Y(R) 13, 25, 46, 85 Villin ID2C3(M) 17, 27, 50, 134, 191 Cytokeratin 7 OV-TL12/30 14, 25, 46, 86, 187 Wilm’s Tumor CAN-R9(IHC)-562(R) 17, 27, 50, 135, 191 Cytokeratin, High MW 34ßE12(M) 14, 25, 46, 90, 187 Cytokeratin, High MW (Basic) AE3(M) 14, 46, 90, 187 Cytokeratin, Low MW AE1(M) 14, 25, 46, 91, 187 Fascin FCN01(M) 14, 25, 47, 97, 188 Villin ID2C3(M) 17, 27, 50, 134, 191 Wilm’s Tumor CAN-R9(IHC)-562(R) 17, 27, 50, 135, 191 Beta-Catenin E247(R) 11, 23, 43, 56 ,184 CA19-9 C241:5:1:4(M) 11, 23, 43, 58, 184 Carcinoembryonic Antigen (CEA) B01-94-11M-P(M) 11, 23, 43, 60, 185 13, 23, 43, 61, 185 8G7G3/1(M) + EPR1600Y(R) + EPR1602Y(R) 17, 27, 146, 191 Actin, Muscle-Specific HHF35(M) 11, 23, 43, 51, 184 Actin, Smooth Muscle 1A4(M) 11, 23, 43, 51, 184 Alpha-Actinin JLN20(M) 11, 43, 52, 184 Caldesmon E89(R) 11, 23, 43, 59, 184 TTF-1 and Cytokeratin 5/6 cocktail MYOSARCOMA PANCREATIC MARKERS Caldesmon,High MW,Smooth muscle h-CD(M) 11, 43, 59 Calponin CALP(M) 11, 43, 59, 184 Carcinoembryonic Antigen (CEA) CEA88(M) Calponin-1 (C-term) EP798Y(R) 11, 23, 43, 60, 184 Carcinoembryonic Antigen (CEA) Polyclonal(R) 11, 43, 61, 185 Myosin Heavy Chains,Smooth Muscle SMMS.1(M) 16, 26, 49, 115 CD56 (Natural Killer Cell,NCAM) NKH-1(M) 13, 45, 75 Chromogranin A LK2H10(M) 13, 24, 45, 82, 186 Cytokeratin 7 OV-TL12/30 14, 25, 46, 86, 187 Cytokeratin 17 E27(M) 14, 25, 46, 88, 187 NEUROBIOLOGY (BRAIN PATHOLOGY) S100 beta EP32(R) 17, 27, 50, 127, 190 S100 Protein Polyclonal(R) 17, 27, 50, 127, 190 S100 Protein 15E2E2(M) 17, 27, 50, 127, 190 E-Cadherin 36(M) 14, 25, 47, 93 Chromogranin A LK2H10(M) 13, 24, 45, 82, 186 Fascin FCN01(M) 14, 25, 47, 97, 188 Chromogranin A PHE-5(M) 13, 24, 45, 82, 187 Glucagon Polyclonal(R) 14, 47, 99, 188 Neurofilament NE-14(M) 16, 26, 49, 116, 190 Insulin EP125(R) 15, 26, 48, 106, 189 Neuron Specific Enolase (NSE) MIG-N3(M) 16, 26, 49, 116, 190 Insulin HB125(M) 15, 48, 105, 189 PGP9.5 3D9(M) 16, 27, 49, 123 S100P EP186(R) 17, 27, 50, 127, 190 Synaptophysin Snp88(M) 17, 27, 50, 129, 191 Synaptophysin Snp88(M) 17, 27, 50, 129, 191 EP211(R) 13, 24, 45, 75, 186 Polyclonal(R) 13, 24, 45, 82, 187 M94138(M) 15, 48, 103 NEUROBLASTOMA NEUROECTODESMAL TUMOR CD56 (Natural Killer Cell,NCAM) Cytokeratin 19 RCK108(M) 14, 25, 46, 89, 187 E-Cadherin EP6(R) 14, 25, 47, 93, 187 PECOMA NKH-1(M) 13, 45, 75 NEUROENDOCRINE POLYPEPTIDES (BRAIN PATHOLOGY) CD63 PERINEURIOMA Glial Fibrillary Acidic Protein (GFAP) GA-5(M) 14, 25, 47, 99, 188 Glial Fibrillary Acidic Protein (GFAP) Polyclonal(R) 14, 47, 99, 188 Substance P Polyclonal(R) 17, 50, 128, 191 Human Chorionic Gonadotropin (hCG) Beta Tau Tau-2(M) 17, 27, 50, 129, 191 PROLIFERATION-ASSOCIATED ANTIGENS Tau Tau-5(M) 17, 27, 50, 129, 191 Cytokeratin 16 EP1615Y(R) 14, 25, 46, 88 EGFR Polyclonal(R) 14, 47, 93 234 Claudin 1 PLACENTAL MARKERS www.biogenex.com Listing by Categories Categories Clone Page Categories Clone Page 12, 23, 44, 64, 185 EGFR(PAN) EP38Y(R) 14, 25, 47, 93 CD5 EP2952(R) Ki-67 EP5(R) 14, 25, 48, 95, 188 CD11C EP1347Y(R) 12, 24, 44, 66, 185 Ki-67 K-2(M) 15, 26, 48, 106, 189 c-erbB-2 (HER-2/neu) CB11(M) 13, 24, 45, 81 Ki-67 EPR3611(R) 15, 26, 48, 107, 189 c-erbB-2 (HER-2/neu) EP3(R) 13, 24, 45, 81 Ki-67 and Lambda Light Chain Cocktail K-2(M) and Polyclonal (R) 15, 26, 146, 189 c-erbB-3 (HER-3) RTJ1/A2(M) 13, 24, 45, 81 EGFR Polyclonal(R) 14, 47, 93 Ki-67 Antigen,Proliferating Cell MIB-1(M) 15, 26, 48, 107, 189 EGFR (PAN) EP38Y(R) 14, 25, 47, 93 Ki-67 Antigen,Proliferating Cell Ki88(M) 15, 26, 48, 107, 189 ErbB-2/HER-2 EP1045Y(R) 13, 24, 47, 95 p105 Proliferation-Associated Nuclear Antigen 2B3(M) 16, 49, 118, Platelet-Derived Growth Factor (PDGF) PDGF88(M) 16, 49, 123 Proliferating Cell Nuclear Antigen (PCNA) PC10(M) 16, 27, 49, 125, 190 Platelet-Derived Growth Factor (PDGF) Polyclonal(R) 16, 49, 124 Stat 5-Alpha (C-term) E289(R) 17, 27, 50, 128,191 Androgen Receptor F39.4.1(M) 11, 23, 43, 53, 184 ZAP-70 ZAP70-C3(M) 17, 27, 50, 135, 191 Bcl-2 E17(R) 11, 23, 43, 54, 184 ZAP-70 YE291(R) 17, 27, 50, 135, 191 PROSTATE MARKERS Bcl-2 Oncoprotein bcl-2/100(M) 11, 23, 43, 55, 184 Bcl-x E18(R) 11, 23, 43, 55, 184 Desmin 33(M) 14, 28, 46, 92,187 CD44 (Phagocytic Glycoprotein1,HCAM) DF1485(M) 12, 45, 72, 186 Dystrophin Dys1(Dy4/6D3)(M) 14, 46, 92, 187 13, 25, 46, 85 Dystrophin Dys2(Dy8/6C5)(M) 14, 47, 92, 187 MG-1(M) 16, 49, 115, 189 Cytokeratin 5 EPR1600Y(R) SKELETAL MUSCLE Cytokeratin 5 & Cytokeratin 14 EP24/EP61(R) 13, 25, 46, 85 Myoglobin Cytokeratin 14 LL002(M) 14, 25, 46, 87, 187 Myoglobin Polyclonal(R) 16, 49, 115, 189 Myosin,Skeletal Muscle MY-32(M) 16, 49, 115, 189 POH-1(M) 16, 49, 119, 190 Cytokeratin cocktail, Broad Spectrum 34ßE12/C51/ AE1(M) 14, 46, 90, 187 Cytokeratin, High MW 34ßE12(M) 14, 25, 46, 90, 187 p34cdc2 (Cyclin Dependent Kinase) Cytokeratin, High MW (Basic) AE3(M) 14, 46, 90, 187 Sarcomeric Actin ZMSA-5(M) 17, 27, 50, 128, 190 14, 25, 46, 91, 187 Vimentin V9(M) 17, 27, 50, 134, 191 LN6(M) 17, 50, 134, 191 Cytokeratin, Low MW AE1(M) Mucin 2 (MUC2) CCP58(M) 15, 48, 113 Vimentin,Non-Hematopoietic P504S (AMACR) 13H4(R) 16, 26, 49, 120, 190 SKIN (ADNEXAL TUMORS) P504S (AMACR) RBT-AMACR(R) 16, 26, 49, 120, 190 Cytokeratin 15 EPR1614Y(R) 14, 25, 46, 88 p63 4A4(M) 16, 27, 49, 121, 190 12, 24, 44, 67, 185 A40010(M) 16, 27, 49, 121, 190 CD15 (Blood group antigen Lewis X) BRA4F1(M) PAP PIN4 Cocktail (P504S+HMW Cytokeratin+p63) 13H4(R)+34ßE12( R)+4A4(M) 16, 27, 147, 190 Epithelial Membrane Antigen (EMA) E29(M) 14, 25, 47, 94, 187 Prostate Specific Acid Phosphatase (PSAP) B01-94-21M-NA(M) 16, 27, 50, 125, 190 Epithelial Membrane Antigen (EMA) Mc5(M) 14, 25, 47, 94, 187 Prostate Specific Antigen(PSA) ErPr-8(M) 16, 27, 50, 126, 190 PSMA EP192(R) 17, 27, 50, 126, 190 RENAL CELL CARCINOMA MARKERS Cytokeratin, Low MW AE1(M) 14, 25, 46, 91, 187 RCC (Renal Cell Carcinoma) RCC-26(M) 17, 27, 50, 126, 190 SALIVARY DUCT CARCINOMA GCDFP-15 EP1582Y(R) 14, 25, 47, 98, 188 Mucin 2 (MUC2) CCP58(M) 15, 48, 113 SEX CORD STROMAL TUMORS Calretinin 2E7(M) 11, 23, 43, 60, 184 Calretinin Polyclonal(R) 11, 23, 60, 184 CD99 EPR3097Y(R) 13, 24, 45, 78, 186 Inhibin-Alpha R1(M) 15, 26, 48, 105, 189 Beta-Catenin E247(R) 11, 23, 43, 56 ,184 Catenin (p120) YE372(R) 16, 26, 44, 61, 185 CD3 epsilon EP449E(R) 12, 23, 44, 62, 185 CD5 4C7(M) 12, 23, 44, 64, 185 SIGNAL TRANSDUCTION PROTEIN internationalcs@biogenex.com SKIN (SPINDLE CELL TUMORS) CD31 (PECAM-1) 9G11(M) 12, 44, 70, 185 CD34 (Endothelial Cell) QBend/10(M) 12, 24, 44, 71, 185 Collagen IV COL-94(M) 14, 46, 83 Cytokeratins 8 & 18 5D3(M) 14, 25, 46, 87, 187 Factor VIII-Related antigen F8 2.2.9(M) 14, 25, 47, 97, 188 Factor XIII Subunit A E980.1(M) 14, 25, 47, 97, 188 Factor XIII A EP3372(R) 14, 25, 47, 97, 188 Glut-1 SPM498(M) 15, 25, 47, 100, 188 NGF Receptor EP1039Y(R) 16, 26, 49, 117, 190 NGF Receptor EP31(R) 16, 26, 49, 117, 190 NKH-1(M) 13, 45, 75 Caldesmon E89(R) 11, 23, 43, 59, 184 Calponin CALP(M) 11, 43, 59, 184 Calponin-1 (C-term) EP798Y(R) 11, 23, 43, 60, 184 CD61 EP2417Y(R) 13, 24, 45, 75, 186 SMALL CELL CARCINOMA OF LUNG CD56 (Natural Killer Cell,NCAM) SMALL, ROUND BLUE CELL TUMORS 235 Listing by Categories Categories Clone Page Categories Clone Page CD63 EP211(R) 13, 24, 45, 75, 186 CD117 YR145(R) 13, 45, 79, 186 CD68 KP1(M) 13, 24, 45, 76, 186 c-myc Protein 9E 10(M) 13. 45, 83, 187 CD68 CD68/G2(M) 13, 24, 45, 76, 186 Mucin 1 (MUC1) EPR1023(R) 15, 26, 48, 112, 189 Myoglobin MG-1(M) 16, 49, 115, 189 NGF Receptor EP1039Y(R) 16, 26, 49, 117, 190 Myoglobin Polyclonal(R) 16, 49, 115, 189 NGF Receptor EP31(R) 16, 26, 49, 117, 190 PGP9.5 3D9(M) 16, 27, 49, 123 p53 EP9(R) 16, 26, 49, 120, 190 Vimentin V9(M) 17, 27, 50, 134, 191 p53 Protein BP53-12-1(M) 16, 26, 49, 120, 190 p53 Protein DO7(M) 16, 27, 49, 121, 190 SOFT TISSUE SARCOMA CD34 (Endothelial Cell) QBend/10(M) 12, 24, 44, 71, 185 p53 Protein 1801(M) 16, 27, 49, 121, 190 CD99 EPR3097Y(R) 13, 24, 45, 78, 186 p63 4A4(M) 16, 27, 49, 121, 190 p63+CK5 Cocktail 4A4(M)+ EPR1600Y( R) 16, 27, 147 PDCD4 EPR3432(R) 16, 27, 49, 122, 190 EP186(R) 17, 27, 50, 127, 190 Aberrant Endothelial Cell 4A11(M) 11, 43, 51 bFGF88(M) 11, 43, 57 Polyclonal(R) 17, 27, 50, 134, 191 CAN-R9(IHC)-562(R) 17, 27, 50, 135, 191 SOFT TISSUE TUMOR Calretinin 2E7(M) 11, 23, 43, 60, 184 Calretinin Polyclonal(R) 11, 23, 60, 184 Desmin 33(M) 14, 28, 46, 92,187 Beta-Catenin E247(R) 11, 23, 43, 56 ,184 Calponin-1 (C-term) EP798Y(R) 11, 23, 43, 60, 184 CD56 (Natural Killer Cell,NCAM) NKH-1(M) 13, 45, 75 bFGF (Basic Fibroblast Growth Factor) Desmin 33(M) 14, 28, 46, 92,187 VEGF PGP9.5 3D9(M) 16, 27, 49, 123 WILM'S TUMOR Heat Shock Protein (HSP-70) BRM-22(M) 15, 47, 101, 188 Heat Shock Protein 27 (HSP 27) G3.1(M) 13, 25, 47, 101, 188 EP1582Y(R) 14, 25, 47, 98, 188 SPINDLE CELL TUMORS UROTHELIAL CARCINOMA S100P VASCULAR ENDOTHELIAL STRESS RESPONSE PROTEIN Wilm’s Tumor SWEAT GLAND CARCINOMA GCDFP-15 THYROID MARKERS Cytokeratin 19 RCK108(M) 14, 25, 46, 89, 187 Cytokeratin, High MW 34ßE12(M) 14, 25, 46, 90, 187 Cytokeratin, High MW (Basic) AE3(M) 14, 46, 90, 187 Cytokeratin, Low MW AE1(M) 14, 25, 46, 91, 187 p21/WAF1 4D10(M) 16, 49, 119, 190 p27 (Kip1) DCS72(M) 16, 49, 119 p27 (Kip1) Y236(R) 16, 26, 49, 119, 190 Thyroglobulin 2H11(M) 17, 27, 50, 130, 191 Thyroid Stimulating Hormone (TSH) 5404(M) 17, 27, 50, 131 Thyroid Stimulating Hormone (TSH) Polyclonal(R) 17, 50, 131 Thyroid Transcription Factor (TTF-1) 8G7G3/1(M) 17, 27, 50, 131, 191 Thyroxine D5(M) 17, 50, 131, 191 TTF-1+GCDFP-15 cocktail 8G7G3/1(M) + EP1582Y(R) 17, 27, 146, 191 AE3(M) 14, 46, 90, 187 TRANSITIONAL CELL CARCINOMA Cytokeratin, High MW (Basic) TUMOR SUPPRESSORS, APOPTOSIS PROTEINS & ONCOPROTEINS Bax Protein Polyclonal(R) 11, 43, 54 Bcl-2 E17(R) 11, 23, 43, 54, 184 Bcl-x E18(R) 11, 23, 43, 55, 184 BRCA1 Protein Polyclonal(R) 11, 43, 55 CD117 T595(M) 13, 24, 45, 79, 186 236 www.biogenex.com General Terms and Conditions 1. 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