AN INTRODUCTION TO THE STUDY OF BURNED HUMAN SKELETAL REMAINS

AN INTRODUCTION TO THE STUDY OF BURNED HUMAN SKELETAL REMAINS The Cyprus Institute Science and Technology in Archaeology and Culture Research Center (STARC) Guide No. 4 Author Efthymia Nikita Reviewers: Version 1.0 Nicosia, 2021 AN INTRODUCTION TO THE STUDY OF BURNED HUMAN SKELETAL REMAINS The Cyprus Institute Science and Technology in Archaeology and Culture Research Center (STARC) Guide No. 4 Author: Efthymia Nikita Reviewers: Colleen Cheverko, Edward Via College of Osteopathic Medicine ∞ Nicholas Herrmann, Texas State University ∞ Simone Lemmers, The Cyprus Institute ∞ Kathryn Marklein, University of Louisville ∞ Ioanna Moutafi, The Malcolm H. Wiener Laboratory for Archaeological Science, American School of Classical Studies at Athens Version 1.0 Nicosia, 2021 Cover photo: Cremation pyre on the bank of Bagamati by Gregor Younger Source: http://www.flickr.com/photos/gregor_y/32072163/ {{cc-by-sa-2.0}} The compilation of the manuscript was made possible through funding from the European Union Horizon 2020 (Promised, Grant Agreement No 811068) and the Research and Innovation Foundation (People in Motion, EXCELLENCE/1216/0023) This work is distributed under a creative commons licence (CC BY-NC 2.0) Nicosia 2021 (Version 1.0) ISBN 978-9963-2858-7-7 CONTENTS 1 Preface 3 Introduction 3 Bone response to fire 5 Commercial cremations versus outdoors pyres 6 Field procedures 7 Laboratory procedures 7 Cleaning 7 Sorting and Cataloguing 8 Minimum Number of Individuals 8 Sex assessment 10 Age-at-death estimation 13 Stature estimation 13 Pathology 13 Trauma 15 Heat-induced fractures 16 Shrinkage and warping 18 Discoloration 20 Fleshed versus dry bone 20 Weights 23 Bone microstructure 25 Crystallinity Index 26 DNA analysis 26 Stable and radiogenic isotope analysis 27 Heat-induced dental alterations 33 References 47 Recording Sheets 6 PREFACE This document is the fourth in a series of guides aimed at promoting best practice in different aspects of archaeological science, produced by members of the Science and Technology in Archaeology and Culture Research Center (STARC) of The Cyprus Institute. The current document was largely developed in the context of two projects: People in Motion and Promised. The implementation of People in Motion involved the laboratory study of a large commingled and partially burned skeletal assemblage from Byzantine Amathus, Cyprus, which came to light in the context of excavations led by the Cypriot Department of Antiquities. Osteological work on this assemblage was co-funded by the European Regional Development Fund and the Republic of Cyprus through the Research and Innovation Foundation (EXCELLENCE/1216/0023). In addition, Promised aims at promoting archaeological sciences in the Eastern Mediterranean, with funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 811068. The study of burned skeletal remains is particularly challenging due to the extensive alteration of the bones, manifesting as warping, discoloration, shrinkage, and fracturing. These macroscopic changes express underlying structural and chemical alterations. As a result, the application of traditional osteological methods (morphological, metric, chemical, molecular, histological and others) is largely inhibited or should be extremely cautious. Nonetheless, the study of burned skeletal assemblages can offer unique insights to funerary practices and technologies, as well as the manipulation of dead bodies. In line with the above, the aim of this guide is to cover various aspects of the study of burned skeletal assemblages. It should be seen as a supplement to the ‘Basic guidelines for the excavation and study of human skeletal remains; STARC Guide no. 1 ’ and the ‘Excavation and study of commingled human skeletal remains; STARC Guide no. 2’. The current guide is meant to serve only as a general outline and the described field and lab-based methods should be modified depending on the context and characteristics of each assemblage under study. A number of excellent volumes have been published in the past years, compiling experimental and case studies on the retrieval and examination of burned skeletal remains in archaeological and forensic contexts (Fairgrieve 2008; Schmidt and Symes 2015; Symes et al. 2012; Thompson 2015). Much of the information presented here has been drawn from these resources, as well as from other publications and the author’s professional experience. References are given throughout the current document but the aim is by no means to provide an exhaustive account of the literature. This document is an open resource and it is anticipated to be updated at regular intervals. I would greatly appreciate any feedback and recommendations for future improvement.* Efthymia Nikita * For feedback/recommendations, please contact me at e.nikita@cyi.ac.cy 1 2 INTRODUCTION Cremation has been a diachronic practice in many different cultures since prehistory, while bodies may also be exposed to (lower) degrees of heat during mortuary practices such as cleansing fires. Besides funerary practices, a human body may be exposed to fire as a result of different events, such as car or aircraft accidents, bombings, natural disasters, homicides and suicides. In forensic contexts fire can also be used to destroy evidence and hinder the identification of the deceased. For these reasons, any anthropologist working in circumstances where Fire Dynamics burned skeletal remains may be encountered Fire is an oxidation reaction that generates heat and should possess a general understanding of light. There are three requirements in order to make a the physical and biochemical alterations bone fire (the so-called ‘fire triangle’): heat, oxygen, and fuel and teeth undergo when exposed to varying (DeHaan 2002, 2015). Heat involves raising the temdegrees of heat. The study of burned human perature of an object to the lowest temperature at remains poses special challenges compared which it will sustain combustion. The amount of oxygen to the anthropological study of non-thermally must be such that can sustain combustion. Finally, the altered bones as exposure to heat produces fuel refers to the combustible materials that are presmacroscopic color changes, shrinkage, fragent and capable of sustaining the fire. The best ‘fuel’ in mentation, and warping, as well as microscopthe human body is subcutaneous fat (DeHaan and Nuric structural and chemical changes to bones. bakhsh 2001). The available amount and interaction of To ensure that these alterations are precisely the parts of the fire triangle will determine the duration analysed and interpreted in their respective and intensity of the fire and, therefore, its impact on a forensic or bioarchaeological context, in turn, body (Devlin and Herrmann 2013). requires a specific approach in the field and in the laboratory. BONE RESPONSE TO FIRE Even when subjected to extreme burning, human bodies cannot be completely destroyed (Bass 1984; Brickley 2007; Eckert et al. 1988; Zana et al. 2017). In general, the effects of fire on human tissue vary based on the proximity of the body to the fire, the temperature reached, and the duration of exposure to the fire (Fairgrieve 2008). It should be remembered that during the thermal exposure of a body, these parameters alter as temperature, heat and ventilation conditions can fluctuate dramatically (DeHaan 2015). In addition, the preincineration condition of bone, that is, the preservation of blood, marrow, moisture, and fat, also influences heat-induced alterations (DeHaan and Nurbakhsh 2001). Myth busting ‘Spontaneous combustion’ and the ‘exploding skull’ are two myths regarding the human body’s response to fire. The former implies the near complete cremation of human bone under unexpected circumstances. Experimental studies have shown that while humans do not spontaneously combust, they are particularly combustible under certain circumstances, such as when bones are osteoporotic (Christensen 2002; DeHaan and Nurbakhsh 2001). With regard to the ‘exploding skull’, contrary to popular opinion (Heglar 1984; Rhine 1998), the cranium does not explode when exposed to prolonged heat. Pope and Smith (2004) found that numerous factors may fracture a burned skull, such as falling debris, the handling of burned remains, the means by which the fire is extinguished, and others, and these external events is what creates the appearance of the exploded skull. The temperature reached during heat exposure depends on the amount of oxygen available, the size and volume of the body, the clothing and other layers surrounding the body, and others (Binford 1963; Christensen 2002; Symes et al. 2015). Soft tissues surrounding the bones have a protective effect as they limit the transfer of heat and restrict oxygen supply to the skeleton. This protective effect depends upon the thickness of soft tissues, thus bones surrounded by thinner layers of soft tissues will be exposed to higher temperatures and levels of oxygen before bones protected by thicker soft tissue layers, which will take longer to be affected by the fire (Fairgrieve 2008; McKinley 2015). 3 A typical outcome of heat-induced shrinkage of muscles, tendons and ligaments is the arrangement of the body in the so-called ‘pugilistic posture’ (Symes et al. 2015; Ubelaker 2009). The ‘pugilistic posture’ will provide further protection for some anatomical regions, hence the pattern described in the previous paragraph may not be observed (Thompson 2015). Figure 1 presents the pattern of thermal destruction of a skeleton at pugilistic posture, indicating the initial, secondary, and final areas to be affected by burning (from Symes et al. 2015). It must be stressed again that Figure 1 provides only a very general pattern, while in reality the sequence of skeletal fire alteration is affected by many parameters, such as the position of the body on the fire, the pre-burning condition of the body, the size of the individual, prior pathological conditions, and many others (Symes et al. 2012). Figure 1. Sequence of skeletal affliction by fire in pugilistic posture (adapted from Symes et al. 2015 Figure 2.7) A literature review regarding the stages of heat-induced bone transformation has been carried out by Mayne Correia (1997) and reviewed by Thompson (2003, 2004). Although both authors identify the same four stages, they occasionally disagree about the temperature intervals at which each stage corresponds. The first stage, dehydration, is characterised by the breakage of the hydroxyl bonds in hydroxyapatite crystals and water loss, leading to subsequent weight reduction and fracturing (Mayne Correia 1997; Thompson 2003). Using scanning electron microscope (SEM) analysis, dehydration is characterized by bubbles in the external lamellae and cracking (Mayne Correia 1997). Both authors agree that dehydration occurs approximately between 100oC and 600oC. The second stage, decomposition, 4 takes place at 500-800oC according to Mayne Correia (1997) and at 300-800oC according to Thompson (2003, 2004). During this stage, organic components decompose and this results in color change, weight loss, reduction in mechanical strength, and changes in porosity. SEM analysis shows an increase in the diameter of the crystals and the lacunae but bone structure is still recognisable (Mayne Correia 1997). In the third stage, inversion, there is an increase in crystal size, the carbonates are Carbonization removed and magnesium is released, causing additional weight loss (Mayne Correia Organic materials contain high proportions of carbon 1997). Under SEM, cracks are wider and the atoms and experience carbonization when exposed to matrix becomes increasingly more homogeintense heat. During heating, complex organic molecules neous, while lacunae become less visible. The break down and elements, such as oxygen and hydrogen, inversion stage occurs between 700oC and are either freed into the atmosphere or combine with 1100oC according to Mayne Correia (1997) other elements, while structural carbon remains. Since and between 500oC and 1100oC based on naturally occurring carbon is black in color, carbonized Thompson (2004). The last stage, fusion, is bone is also black (Symes et al. 2015). characterised by the melting and coalescence of the crystal matrix (Thompson 2003). An Calcination increase in crystal size can be observed, and During calcination, the freed carbon from organic molconsiderable bone dimensional reduction ecules is combined with oxygen and forms carbon diand an increase in mechanical strength take oxide (CO2) or carbon monoxide (CO). Subsequently, place (Thompson 2004). This stage occurs it is released into the atmosphere. The remaining bone at 1600oC+ according to Mayne Correia is comprised of inorganic components, thus its color is (1997) but merely at 700oC+ according to white because this is the natural color of hydroxyapatite Thompson (2004). Note that these four (Mayne Correia 1997; Mayne Correia and Beattie 2002; stages ‘in themselves do not explain all of the Thompson 2004, 2005). Fracturing, shrinkage, and fundamental causal changes occurring within warping accompany calcination (Schwark et al. 2011; hard tissues, and to date are entirely theoretiThompson 2004, 2005). cal’ (Thompson 2004, p. 203). Commercial cremations versus outdoors pyres Given the importance of the environment in which fire exposure takes place, it is relevant to outline how modern cremations compare to outdoors pyres. Modern commercial cremations take place in gas-fired ovens, where the main chamber is lined with heat-resistant refractory bricks (Davies and Mates 2005). The body is placed inside a body bag, a cardboard box, or a wooden coffin. The temperature is typically 870°C–980°C and the average duration 2–2.5 hours (Rosen 2004). Afterwards, the burned remains are pulverized to reduce further their volume, leaving little diagnostic bone (Symes et al. 2013). By contrast, in outdoors pyres, which require human intervention and constant heat sources, most of the heat is lost to the atmosphere, a constant external heat source is necessary, and the temperature cannot stay uniform throughout the process. A further complicating factor is the wind, the strength and directionality of which will affect heat distribution and temperature maxima. In addition, the heat is directed to the body only from below, whereas in the cremator heat exposure is multi-directional (McKinley 1994a). Once the main pyre structure burns down, the remains will rest on the hot ash bed, and the cremation may continue for several more hours (McKinley 2006). Weather conditions play an important role in the duration for which the pyre will burn: strong winds will make the pyre burn faster but unevenly, while rain will reduce the pyre temperature or even extinguish it (McKinley 2015). The vegetation surrounding the pyre is also important: dry vegetation will increase fire temperature but reduce its duration due to the fast consumption of the fuel; oily vegetation will take longer to ignite and the pyre duration will be prolonged, while with wet vegetation, a fire may not ignite (Symes et al. 2013). 5 FIELD PROCEDURES Burned remains may be found in various contexts: on the ground surface, (partially) buried, inside funerary structures, etc. Any field procedure has to be adjusted to the unique challenges posed by each context of recovery and the associated degree of preservation of the remains, the sample size, and the degree of commingling. In some contexts, a confined excavation will be appropriate, while in others, surface surveying will be a necessary first step to document the spread of the remains. Burned human remains pose two additional and interconnected challenges compared to unburned remains: fragmentation and identification. Burning can lead to extreme bone fragmentation, which hinders recovery in the field. This extreme fragmentation coupled with the morphological, chemical and structural deformities that characterise burned bone, often renders the differentiation between such bone and other materials difficult (Fairgrieve 2008; Ubelaker 2009). The steps in the recovery of burned remains are summarised in the box below (see also Nikita et al. 2019). During each of these steps, it is important to describe in detail any features and the stratigraphy, including plan and profile maps, as well as measurements. Any post-depositional disturbance should be recorded in detail as well, while soil samples should be taken and sample locations should be documented. The excavation, documentation and recovery should continue until undisturbed strata are visible (Devlin and Herrmann 2013; Fairgrieve 2008). Steps in the excavation of burned remains (Naji et al. 2014; Schmidt 2015) 1. Identify the extent of the deposit. 2. Photograph and draw the deposit. 3. If the remains are contained in an urn or other pot, transport the pot to the laboratory so that it is excavated in a controlled environment by an osteoarchaeologist. If the remains are not contained in a pot (or in some structure that may be lifted as a block and transported to the lab), excavate them in the field. 4. Construct a reference grid over the deposit. 5. Document and collect all surface findings (i.e., skeletal elements, artifacts etc.). 6. Using a trowel, paint brushes and wooden tools, excavate the burned remains in layers defined by all the fragments that can be removed without disturbing the underlying level (Fairgrieve 2008). 7. Map large fragments (> 3 cm) individually and group smaller ones (< 3 cm) by grid square. 8. Bag separately the remains of each layer. Make sure to label accordingly fragments found in close proximity so as to facilitate reconstructions and fragment identification later on in the lab. 9. Use paper towels to wrap fragile bones prior to transportation and place them inside paper bags. Avoid plastic bags or metal cans as the former encourage moisture and the latter contribute to further fragmentation. 10. Sieve all soil, ideally using a 3mm screen. Note that for burned remains found inside urns or other contained structures, the use of virtual approaches, such as computed tomography, has proven particularly useful as a first non-invasive step to assess the context prior to destructive micro-excavation (Higgins et al. 2020). 6 LABORATORY PROCEDURES As with field procedures, the particular laboratory procedures employed need to be assemblage-specific. The following sections provide a broad outline. Cleaning Bones and teeth should be rinsed with tap water, when not too fragile, and left to dry naturally, avoiding direct sunlight. Dry brushing is preferable when cleaning carbonized bone, as its charcoal-like consistency is more friable than calcined bone. Where bone is well preserved, wet sieving using a 1mm mesh size is ideal. If necessary, a soft toothbrush or a wooden cocktail stick may be used to remove adhering dirt (Fairgrieve 2008; Schmidt et al. 2015). Sorting and Cataloguing The bone inventory as a means of assessing the When sorting bones, as much detail as pospre-burning condition of remains sible should be used in identifying the anatomical location of each fragment, e.g. ‘proxWhen the representation of anatomical regions is atypimal foot phalanx head and shaft’ (McKinley ical, it is most likely that the cremation involved dry 2017). Particularly useful in this respect is the skeletons than fleshed bodies. When fleshed bodies zonation system for partially preserved reare burned, bone loss may occur during the recollecmains, which divides each skeletal element in tion of the remains from the pyre after cremation and/ sections/zones (Knüsel and Outram 2004 – or during their transportation to the final deposition see also STARC Guide No. 2). Subsequently, site. If dry bones are burned, bones may be lost during the percentage preservation of each zone the abovementioned processes as well as before cremay be recorded. If it is not possible to idenmation, when the bones are recovered from their origtify their anatomical location, bone fragments inal deposition site (Godinho et al. 2019a). In assessing should be sorted into broader categories (e.g. the pre-burning condition of the skeleton based on the cranium, thorax, pectoral girdle, upper limb, bone/tooth inventory, it is particularly relevant to note pelvic girdle, lower limb, unidentifiable) (Devlin the presence of small skeletal elements, such as tooth and Herrmann 2015). When bones are too roots or phalanges, as these are the elements most likely partially preserved, they should be classito be left behind when skeletonized remains are transfied into even broader groups, such as ‘flat,’ ported (Lemmers 2012; Masotti et al. 2020). ‘short,’ ‘epiphysis,’ or ‘diaphysis.’ Due to the friable nature of burned remains, the number of fragments may increase due to handling; hence, recording weights is important (see section Weights) (Fairgrieve 2008). Fragments that are particularly small could be divided into size groups and weighted per group (Naji et al. 2014; Schultz et al. 2015). Each identifiable bone and tooth fragment and each fragment with length over 2 cm should receive a catalog number. The use of ink on the remains should only be done if the bone is not going to be subjected to chemical tests and if such writing will not impede the visibility of important anatomical traits. Due to the friable nature of burned remains, it is preferable to number the bone containers (e.g. bags) rather than the bones themselves (Fairgrieve 2008; Watson et al. 2015). Reconstruction Although often impossible to undertake due to the warping of bone fragments, reconstruction/refitting (the articulation of the broken edges of bone fragments) enhances the morphological and metric study of burned remains and can provide important information regarding the dispersal of fragments from a single element across the deposit (Curtin 2015; Ubelaker 2009). Consolidants should be used in moderation, with preference to water- or alcohol-soluble materials (Schmidt 2015). 7 Minimum Number of Individuals Burned remains may represent multiple skeletons because many individuals had been burned together or because the pyre area had been used multiple times and debris from successive cremations got mixed or for other reasons (André et al. 2013). The identification of multiple individuals in the same deposit is based on supernumerary bones or skeletal elements with discordant dimensions, age, sex or systematic disease (Irish et al. 2015; Rubini et al. 1997). Note, however, that bone dimensions should be used cautiously because heat exposure results in shrinkage and warping (see section Shrinkage and warping). The best approach is to estimate the Minimum Number of Individuals (MNI) using an anatomical area that is particularly dense and less likely to be affected severely by fire, such as the petrous part of the temporal bone (Fairgrieve 2008). The use of more elaborate methods, such as those for the estimation of the Most Likely Number of Individuals (Adams and Konigsberg 2004) or the Initial Number of Individuals (Nikita and Lahr 2011), is often not practical given the very small burned fragments that comprise the majority of cremated assemblages. In cases of partial cremation, however, such methods may be applied in conjunction to MNI estimates. DNA analysis may also be adopted to sort skeletal elements per individual (Schultz et al. 2015); however, DNA tends to be destroyed above 600°C and often even below that temperature (Walker et al. 2008). Another means of estimating the number of individuals present in an assemblage is the average weight of skeletal remains. The average weight per adult body in modern crematoria is 1,760.3 g to 3,379 g (Bass and Jantz 2004; May 2011; Van Deest et al. 2011). Any greater mass of burned remains supports strongly the presence of more than one individual. Any smaller mass of remains, however, does not necessarily imply that only one individual is represented in the assemblage; instead it may indicate some selection process. Another limitation of this approach is that cremation weights vary greatly based on an individual’s age, sex, pathological status and many other factors (Bass and Jantz 2004), while taphonomic factors may alter considerably the weight of burned bone (Amarante et al. 2019). See also section Weights. Sex assessment Many authors have argued that techniques used for sexing unburned skeletal remains based on the morphology of the skull and pelvis are applicable to cremated individuals (Geber et al. 2017; Lara et al. 2015; Mayne Correia and Beattie 2002; Rubini et al. 1997; Wahl 2015). Nonetheless, Fairgrieve (2008) highlights a number of limitations in the applicability of these criteria due to shrinkage, warping and heat-induced fractures (see sections Shrinkage and warping and Heat-induced fractures). The use of osteometric methods to sex burned human remains has also been suggested. Gejvall (1969) proposed metric standards for sex estimation based on skull thickness, humeral head diameter, and femoral, humeral and radial diaphyseal thickness. Warren and Maples (1997) also used femoral and humeral head measurements of burned human remains and found that femoral head measurements had an average of 44.2 mm for males and 38.2 mm for females, and humeral head measurements had an average of 45.8 mm for males and 38.2 mm for females. Van Vark (1975) and Van Vark et al. (1996) examined the expression of different cranial and post-cranial sexually dimorphic traits on a cremated sample from 19th century Amsterdam. The results were extremely good for the male individuals (92% correct classification) and reasonable for the female individuals (79% correct classification). In addition, Schutkowski (1983) and Schutkowski and Herrmann (1983) obtained correct sex classification in 67.0% 73.4% of the cases examined using discriminant function analysis on the petrous bone. Similarly, Gonçalves et al. (2013a) achieved successful sex classification scores using humeral, femoral, talar and calcaneal measurements from Portuguese cremated individuals. However, other metric sexing methods, such as the lateral angle of the internal auditory canal, have been found not to provide accurate results with burned human skeletal remains (Gonçalves et al. 2015a; but see Masotti et al. 2019). It must be stressed that metric sex estimation is based on population-specific samples, so the application of such methods should be cautious, whether in burned or unburned remains. 8 Logistic regression equations for sex estimation (adapted from Tables 6 and 7 in Gonçalves et al. 2013a)* y y y y y y y y y -32.753 + 0.891 * Humeral head transverse diameter -26.919 + 0.661 * Humeral head vertical diameter -49.415 + 0.904 * Humeral epicondylar breadth -29.896 + 0.782 * Femoral head transverse diameter -30.376 + 0.759 * Femoral head vertical diameter -32.849 + 0.683 * Talar maximum length -39.628 + 0.549 * Calcaneal maximum length -37.626 + 0.825 * Humeral head transverse diameter + 0.177 * Humeral head vertical diameter -36.860 + 0.664 * Femoral head transverse diameter + 0.299 * Femoral head vertical diameter *Positive values suggest males, while negative values suggest females More recently, Cavazzuti et al. (2019) proposed cut-off points for osteometric sexing in archaeological populations (Table 1). To develop their method, the authors used Bronze Age and Iron Age cremated individuals from Italy. An important caveat of their study is that the sex of these individuals was assessed on the basis of ‘clearly engendered grave goods’, thus it was based on the assumption that gender was highly correlated to sex in these groups. Gouveia et al. (2017) examined the potential of odontometric sex estimation on experimentally heated teeth and found that this approach has serious limitations, though certain dimensions and combinations of variables (cementum-enamel junction perimeter, combined mesiodistal and buccolingual diameters) may reach correct sex classification that exceeds 80%. In contrast, Godinho et al. (2019b) found that micro-fracturing produces statistically significant expansion of the tooth crown, which impacts odontometric sex estimation. The authors highlighted that the effect of heat-induced size changes may be removed using μCT scanning; however, this is a complex approach and, coupled with the fact that tooth crowns tend to fracture when exposed to high temperatures, renders odontometric sexing impractical. Finally, studies using the weight of burned remains to discriminate between sexes (Van Deest et al. 2011) should be considered tentative at best (Naji et al. 2014). Although the difference between female and male weights is often statistically significant, the range of variation and the often incomplete state of the skeleton in burned assemblages due to various taphonomic processes additionally to heat exposure, strongly limit sex assessment using cremated weights. In addition, age-related differences also interfere with relevant assessments (Gonçalves 2011). See also section Weights. Table 1. Cut-off points for osteometric sexing based on ancient Italian populations (adapted from Cavazzuti et al. 2019 Tables 3 and 4)* Skeletal Element Measurement Cut-off point (mm) Mandible condyle width 15.87 Axis dens anteroposterior diameter 9.55 Axis dens transverse diameter 9.10 Humerus vertical head diameter 37.88 Humerus trochlea maximum diameter 20.00 9 Skeletal Element Measurement Cut-off point (mm) Humerus trochlea minimum diameter 13.28 Humerus capitulum maximum diameter 16.09 Radius head maximum diameter 18.32 Lunate maximum width 14.30 Lunate maximum length 13.82 Femur vertical head diameter 39.39 Patella maximum height 35.68 Patella maximum width 36.61 Patella maximum thickness 16.10 Talus maximum length 46.87 Talus head-neck length 16.51 Talus trochlea length 28.92 Talus trochlea width 27.52 Navicular maximum lenght 13.46 First metatarsal dorsoplantar width of the head 16.17 First metatarsal mediolateral width of the head 17.02 Key: Values greater than the cut-off point suggest males, while smaller values suggest females; Measurements in bold are those that showed accuracy equal to or higher than 80% in the cross-validation analysis. Age-at-death estimation To determine age-at-death in burned remains, scholars use the same osteological methods as for unburned skeletal remains (Geber et al. 2017; Irish et al. 2015; Lara et al. 2015; Mayne Correia 1997; Mayne Correia and Beattie 2002; Rubini et al. 1997), unless the remains are so partially preserved that it is possible to only distinguish between ‘nonadult’ (<18 years) and ‘adult’ (>18 years) (Curtin 2015; McKinley 2017). Even in cases where traditional ageing methods can be applied, one needs to bear in mind the impact of heat-induced alterations (Table 2). Special reference should be made to metric and histological methods and their applicability to ageing burned remains. When using metrics to age burned nonadults, it should be remembered that fire exposure results in a reduction in bone dimensions. Fazekas and Kósa (1978) make reference to the study by Petersohn and Köhler (1965) where percent shrinkage was examined on fetal bones as these passed through the fresh, carbonized and calcined states. Huxley and Kósa (1999) reevaluated Petersohn and Köhler’s (1965) data and noted that shrinkage from carbonization and calcination exhibits great variation depending on the lunar month and the skeletal element. Table 3 is a compilation by Huxley (1998) of Petersohn and Köhler’s (1965) data showing the differences in the average percent shrinkage of different skeletal elements per lunar month. 10 Table 2. Influence of heat-induced changes on age-at-death estimation methods (adapted from Fairgrieve 2008 Table 5.6) Ageing method Heat-induced alteration Primary ossification centers Destruction, especially of infant wrist and cranial bone cartilaginous ossification centers Tooth formation & eruption Desiccation of tooth crown and roots Epiphyseal fusion Fracturing of epiphysis and fusion sites, destruction of growth plate Pubic symphysis morphology Fracturing, warping, bone surface deformity Auricular surface morphology Fracturing, warping, bone surface deformity Sternal rib ends morphology Fracturing, destruction Cranial suture closure Fracturing, delamination Table 3. Comparison of shrinkage rates (%) by skeletal element for fetuses between 4–10 lunar months (LM) and newborns (drawn from Huxley 1998 Table 7; reprinted by Fairgrieve 2008 Table 5.5) 4LM 5LM 6LM 7LM 8LM 9LM 10LM Newborn Humerus 9.13 5.39 3.37 2.24 1.45 1.68 1.75 2.03 Radius 9.73 5.79 4.30 2.24 2.41 1.90 1.70 0.39 Ulna 9.23 5.65 3.46 2.25 2.21 1.82 3.09 1.06 Femur 13.85 4.59 3.56 2.46 2.28 1.67 1.72 1.48 Tibia 12.35 5.82 3.44 2.51 2.93 1.94 1.69 1.19 Fibula 6.27 7.18 2.77 2.07 1.82 1.59 1.46 1.52 With regard to histological methods, Bradtmiller and Buikstra (1984) found that bone burned at 600oC could provide accurate microscopic age estimates. More recently, Absolonova et al. (2013) tested the effect of higher temperatures of exposure (700°C, 800°C, 1000°C) on bone microstructure and found a decrease in the dimensions of the microstructures and an increase in their number per mm2 induced by age, but also by burning within the same age group. The authors highlighted that burning-induced microstructural changes are similar to age-related changes, which reduces the applicability of age-at-death estimation equations, especially for remains burned at temperatures over 800°C. For this reason, the authors developed new regression equations for unburned bones, bones burned at 700°C, and at 800°C (Tables 4-5). 11 Table 4. Histomorphometric variables used in age-at-death estimation equations (adapted from Absolonova et al. 2013 Table 2) Variable Abbreviation and Unit Number of intact osteons per mm² POC_OST; #/mm²* Osteon circumference OBV_O; μm Maximal osteon axis MAX_O; μm Haversian canal feret diameter F_PR_K; μm Haversian canal shape factor SFAC_KAN Number of non-Haversian canals per mm² P_NON_H; #/mm² Percentage of total internal circumferential lamellae area PR_LAM; % *#/mm² is the number of microstructures per mm² of the compact bone area Table 5. Age-at-death prediction equations for pooled sexes (adapted from Absolonova et al. 2013 Tables 5-7) Fire temperature Equation r SEE Unburned Y = 163.0256 - 0.1449(OBV_O) - 0.4786(PR_LAM) 6.7111(P_NON_H) 0.761 13.924 700°C Y = -59.8169 - 0.2822(MAX_O) + 222.6290(SFAC_KAN) 0. 673 16.248 800°C Y = 100.5203 - 0.353(MAX_O) + 0.4769(F_PR_K) + 0.4136(POC_OST) 0.578 14.903 The accuracy of histological methods It must be noted that even in unburned remains, the use of histological age-at-death estimation should be cautious with many scholars pointing out that histological methods should merely be used as a rough age-at-death indication (e.g. Chan et al. 2007; García-Donas et al. 2016; Lagacé et al. 2019; Paine and Brenton 2006). Heat exposure also affects the identification of dental age-at-death indicators, such as the apposition of secondary dentin, dentinal sclerosis and cementum incremental lines (Gocha and Schutkowski 2013; Naji et al. 2014). 12 Stature estimation The application of methods of stature estimation based on fragmentary bones has been attempted by various researchers studying burned remains (Lisowski 1968; Malinowski and Porawski 1969; Piontek 1975; Rösing 1977). However, these studies recognize that the error rates for such estimates are large due to bone shrinkage (Holland 1992; Mayne Correia 1997). Fairgrieve (2008) suggests the adoption of a correction factor to account for bone shrinkage when applying one of the various methods available for stature estimation. However, the degree of bone shrinkage is usually unknown. Thus, stature estimates should only be attempted for skeletal elements that appear to have had limited heat exposure. Pathology Shrinkage, warping and fragmentation obscure pathological assessments. However, certain types of pathology, such as degenerative disease and porotic hyperostosis, can be reliably identified even on small burned fragments (Reinhard 1994). A key issue with the study of burned remains is the identification of trauma, as detailed below. Trauma Even though in burned remains, sharp-force, blunt-force and ballistic trauma may be difficult to discern, experimental studies indicate that diagnostic evidence often survives heat exposure. Herrmann and Bennett (1999) showed that sharp-force trauma can be recognized after incineration (see also Figure 2), but this was not the case for ballistic trauma due to the extensive fragmentation it generated prior to burning. Blunt-force trauma could be identified in most cases but required the reconstruction of the skeletal elements prior to any assessment. The authors also noted that larger bone fragments were associated with traumatic fracturing whereas smaller fragments and perpendicular fracture angles with heat-induced fracturing. Regarding longitudinal fractures, smooth surfaces were most frequently associated with traumatically induced fractures. Looking into different types of sharp-force trauma, de Gruchy and Rogers (2002) found that chop marks could be identified on burned bone, whereas hacking made the bone more prone to heat-induced fragmentation. In the same direction, Kooi and Fairgrieve (2013) concluded that temperature, fuel, oxygen availability and general burning environment affect cut mark preservation; however, they also stressed the shielding effect that soft tissue has in low temperatures. In contrast, Waltenberger and Schutkowski (2017) concluded that despite heat-induced alterations, cut marks can be identified on burned bones, as the variables principally affected are the slope height and floor angle of the cut, whereas the depth, width, slope, opening angles and floor radius are not significantly influenced by fire. This is in agreement with Tutor et al. (2021), who examined experimentally inflicted sharp-force trauma by means of a machete and a serrated knife on pre-burned bones and were able to distinguish cut marks from heat-induced alterations, though some heat-induced fractures (e.g. step and transverse fractures) can be mistaken with trauma. In contrast, cut marks inflicted again with a machete and a serrated knife but this time on bones that were subsequently burned, were largely eliminated after heat exposure (Tutor et al. 2020). With regard to saw marks, Marciniak (2009) showed that heat exposure affected the identifiability of saw mark striae, but the marks left by most types of handsaws and power saws could be discriminated, and Robbins et al. (2015) found that the saw striae characteristics identified in burned samples using SEM and stereomicroscopy were in agreement to each other. Collini et al. (2015) analysed blunt-force trauma, drill injuries and gunshot wounds in experimentally charred bones. Morphological trauma features were overall preserved after heat exposure, but depressed fractures increased in dimensions, drilled injuries shrunk, and the number of fractures increased in samples with gunshot wounds. Finally, Pope and Smith (2004) examined cranial trauma and found that identification of ballistic, blunt-force, and sharp-force wounds is possible, as detailed in Table 6; in contrast, Franceschetti et al. (2021) concluded that peri-mortem cranial fractures are rarely possible to identify post-cremation. 13 Figure 2. Pre-burning sharp-force trauma – note sharp margins compared to irregular margins of fire-induced fractures (adapted from Devlin and Herrmann 2013 Figure 16.13) Table 6. Summary of heat effects on cranial trauma (adapted from Pope and Smith 2004 Table 2) 14 Type of trauma Heat-related bone changes Trauma signatures in burned crania ballistic • Focal retraction and shrinkage of wounds • Advanced destruction of exposed injuries • Accelerated bone color changes in open injuries • • • • • blunt-force • Focal retraction and shrinkage of impact sites • Advanced destruction of exposed injuries • Accelerated bone color changes in open injuries • Impact sites exhibiting tool marks or inwardly crushed bone • Secondary radiating or concentric fractures • Juxtaposition of color in adjacent fragments • Radiating fractures into green bone • Deformed, ragged, or eroded fracture margins • Depression, inward crushing, and tool marks sharp-force • Focal retraction and shrinkage of incisions • Advanced destruction of exposed injuries • Accelerated bone color changes in open injuries • Linear incisions, depressions, cuts, chops, saw marks, punctures, stabs, hacks, drill marks, and other tool marks control • Bone color changes according to degree of heat exposure • Delamination, fragmentation, embrittlement, and color changes • “Exploded appearance” Internal or external beveling Secondary radiating or concentric fractures Juxtaposition of color in adjacent fragments Radiating fractures into green bone Deformed, ragged, or eroded fracture margins Heat-induced fractures Heat-altered bone gradually loses its water and organic components, and becomes susceptible to compressive and tensile forces, resulting in heat-induced fractures (Mayne 1990). The degree of bone fragmentation depends upon three factors: fire temperature, fire duration, and existence of mechanical trauma. In temperatures under 700°C, there is little fragmentation, except in the long bone epiphyses (Bohnert et al. 1998; Pope and Smith 2004), while at temperatures over 700-800°C, bones become more fragile (Marella et al. 2012; Pope and Smith 2004). Even though fire duration is important, greater bone fragmentation does not necessarily imply a longer duration of fire exposure. DeHaan (2012) experimented with a seven-hour fire and found that the head and upper limbs were left largely intact. Similarly, Spitz (1993) found identifiable bone fragments even after one or two days of fire exposure. Symes and colleagues (2013, 2015) discuss at length the biomechanics of burned bone and propose seven classes of heat-induced fractures (Figures 3-4): 1. Longitudinal: Longitudinal fractures are the most common burn fractures in long bones. They are usually parallel to the osteon canals, although they may also exhibit a somewhat helical direction down the long bone axis. They may penetrate the marrow cavity. 2. Step: Step fractures extend transversely from one longitudinal fracture to another. 3. Transverse (or straight transverse): Transverse fractures are very common; they are perpendicular to the long bone axis. They tend to penetrate the medullary cavity and may completely transect the bone. They are very similar to or comprise step fractures. 4. Patina: Patina fractures are superficial and appear as a mesh of uniform small cracks. They are found mostly on the flat surfaces of postcranial bones, the epiphyses and the cranial bones. 5. Splintering and Delamination: These fractures are expressed as a separation of cortical from trabecular bone, and they are mostly found in the cranial bones, the epiphyses and the costochondral rib ends. 6. Burn line fractures: These fractures separate the burned from the unburned bone surface. 7. Curved transverse (curvilinear): These fractures circumscribe the long bone shaft. They may extend from longitudinal fractures or show an oblique orientation. A less common type manifests as ‘concentric rings.’ Figure 3. Patina fracture with transverse, longitudinal and curved transverse fractures (left) (adapted from Symes et al. 2013 Figure 14.16), transverse fractures (middle) and delamination (right) (adapted from Devlin and Herrmann 2013 Figures 16.6 and 16.8) 15 Figure 4. Curved transverse fractures at distal femur (left) (adapted from Symes et al. 2013 Figure 14.10) and unidentified long bone (right) (adapted from Gonçalves et al. 2011 Figure 2) Recording fragmentation The maximum length of each bone fragment should be recorded using a sliding calliper (Bontrager and Nawrocki 2015; Watson et al. 2015). Subsequently, the percentage of bone fragments smaller than 1 or 2 cm indicates the intensity of fragmentation (Curtin 2015; Lyman 1994). For example, Minozzi (2015) sorted the degree of bone fragmentation into three categories: high if more than 50% of the remains were smaller than 2×2cm, medium if more than 50% of the remains were larger than 2×2cm, and low if more than 50% of the remains were larger than 5×2cm. Alternatively, the relative fragmentation of bone can be estimated by the ratio of the number of fragments recovered to the minimum number of elements. The higher this value, the more fragmented the bones (Costamagno et al. 2005; Lyman 1994). For particularly fragmented remains, the Fragmentation Index proposed by Harvig and Lynnerup (2013) may be used. This is the ratio of cremation weight (in grams) to cremation volume (in millilitres). A Fragmentation Index below 1 represents limited fragmentation, whereas a Fragmentation Index above 1 represents substantial fragmentation. Shrinkage and warping Heat-induced dimensional change (bone shrinkage) has been noted in a number of early experimental studies (Dokládal 1971; Malinowski and Porawski 1969; Piontek 1975). Among the most systematic early studies were those by Herrmann (1976, 1977), who heated cortical bone at 150°C to 1200°C and identified three phases of shrinkage: 1. 150-300°C, resulting in 1-2% shrinkage 2. 750-800°C, also resulting in 1-2% shrinkage 3. 1000-1200°C, resulting in 14-18% shrinkage More recently, Byers (2005) confirmed the above results as he found minimal to 2% bone shrinkage at temperatures up to 700oC, 1-2% shrinkage at temperatures of 700-800oC, and 10-15% shrinkage at temperatures over 800oC. Subsequent studies using different skeletal elements and temperatures have concluded that the expected shrinkage 16 in cremated bone is limited up to temperatures of 800°C; however, above this temperature, shrinkage should be taken into consideration when applying morphological and metric skeletal methods (Bradtmiller and Buikstra 1984; Buikstra and Swegle 1989; Grupe and Herrmann 1983; Holland 1989; Hummel and Schutkowski 1986; Nelson 1992; van Vark 1970). Thompson (2005) found a wide range of dimensional changes on bones burned at different temperatures (500o, 700o, 900o) for different lengths of time (15’, 45’) and measured at different points after removal from the furnace (5’, 15’, 25’): -4.5% to 13.0% dimensional change for bones burned up to 500oC, -1.7 to 19.3% for bones burned up to 700oC, and -3.9% to 37.7% for bones burned at 900oC. Thompson’s (2005) results highlighted that the duration of fire exposure is also important. Gonçalves’ (2011) experimental work supported this statement but stressed that the relationship between shrinkage and exposure duration is not linear. As for the relationship between bone mineral content and shrinkage, Herrmann (1976, 1977) found higher percent shrinkage for males in comparison to females, which he associated to higher percentages of bone mineral in the former. In contrast, Huxley and Kósa (1999) found decreased shrinkage of heat-exposed bones with increasing age in foetuses; therefore, a negative association between shrinkage and bone mineralization (Guo 2001). In addition, mean shrinkage rates in foetuses have been found by many authors to exceed those seen in adults due to the relatively higher proportion of collagenous distribution within developing nonadult bones (Bradtmiller and Buikstra 1984; Harsányi 1993; Herrmann 1977; Holland 1989; Müller et al. 1952). These findings support that skeletal elements become less susceptible to heat-induced shrinkage as bone mineralization progresses. Another important factor determining the degree of shrinkage is bone structure. Hummel and Schutkowski (1986) found that temperatures up to 1000oC resulted in 5% shrinkage in bone length but 27% reduction in cross-sectional bone diameter. Similarly, Thompson’s (2005) experimental study on sheep long bones recorded more shrinkage in the epiphyses and attributed this finding to the random arrangement of collagen fibers in trabecular bone. As Thompson (2005) notes, there is a discrepancy in earlier literature regarding which type of bone shrinks more with Gejvall (1969) and Gilchrist and Mytum (1986) arguing that compact bone will shrink the most and McKinley (1994b) and Van Vark (1974) arguing that trabecular bone will exhibit more shrinkage. The author stresses that both arguments may actually be correct: it may be that trabecular bone exhibits more absolute shrinkage but compact bone shows more relative shrinkage. As mentioned above, the critical temperature at which the degree of heat-induced shrinkage significantly increases has been set at around 800oC (Buikstra and Swegle 1989; Eckert et al. 1988; Holland 1989; Spennemann and Colley 1989). This 800oC threshold lies near the beginning of the fusion stage of heat-induced bone transformation (see above), which is characterised by the coalescence of the inorganic phase and the filling in of the pores left by the freed water and organic phase. This is the process that generates a reduction in bone size (Thompson 2005). Even though shrinkage can be notable, a more pronounced heat-induced change is warping, that is, a deformity in the natural bone shape (Bontrager and Nawrocki 2015) (Figure 5). Binford (1963) attributed warping to the contraction of muscle fibres, while Spennemann and Colley (1989) linked it to the entrapment of heat inside the medullary cavity. More recently, Thompson (2005) associated warping to the contraction of the periosteum and to the different distribution of collagen within bone. Following the last hypothesis, Gonçalves et al. (2011) argued that warping would depend on bone collagen preservation, thus it would not be related to the presence of soft tissues. Figure 5. Warped tibia (adapted from Gonçalves et al. 2011 Figure 1) 17 Discoloration The varying colors encountered on burned bones have received great attention as color can provide information regarding the temperature of the fire, oxygen availability, the preincineration state of bone, the position of the body on the pyre, and chemical interactions with soil (Bennett 1999; Bennett-Devlin et al. 2006; Binford 1963; Bonucci and Graziani 1975; De Graaff 1961; DeHaan and Nurbakhsh 2001; Hummel et al. 1988; Pope 2007; Shipman et al., 1984; Thompson et al. 2017; Walker et al. 2008; Wärmländer et al. 2019). It must also be remembered that color may be altered by other materials in the firing environment; for example, a proximity to metals may produce green, yellow, pink and red discoloration (Dunlop 1978). With increasing heat exposure, bone progresses from tan to dark brown to black, then blue, gray, and finally white (Figure 6) (Baby 1954; Bennett 1999; Binford 1963; Buikstra and Swegle 1989; Gilchrist and Mytum 1986; McCutcheon 1992; McKinley 2000; Nicholson 1993; Shipman et al. 1984; Stiner et al. 1995). As thermal damage progresses from the external bone surfaces to the internal ones, colour gradients may be identified across the bone, such as the so-called ‘sandwich effect’, whereby the external bone surface is white and the internal grey or black (Symes et al. 2015). As briefly discussed in section Bone response to fire, when the body is fleshed when exposed to heat, the pugilistic posture (Figure 1) will shield certain anatomical parts and expose others, also leading to patterned color alterations on the skeleton (Symes et al. 2015). Deviations from this expected pattern may offer insights to the pre-incineration condition of the body, e.g. the use of binding or other means that result in an unusual body position in the pyre (McKinley 2015). The work by Shipman et al. (1984) was the first to standardize burned surface color descriptions using Munsell soil color charts. Since then, different authors have proposed slightly different temperatures at which each color change occurs, even though the progression of colors is largely always the same (e.g. Bonucci and Graziani 1975; Holden et al. 1995a, 1995b; McCutcheon 1992; Munro et al. 2007; Walker et al. 2008). Figure 6. Bone fragments illustrating color changing sequence from unaltered (far left) to dark brown, black, gray, and white (adapted from Devlin and Herrmann 2013 Figure 16.3) It must be remembered that heat-induced bone color change is a macroscopic alteration linked to chemical and physical changes in bone structure (Bonucci and Graziani 1975; Devlin and Herrmann 2015; Ellingham et al. 2015; Fairgrieve 2008; McCutcheon 1992; McKinley 2000; Munro et al. 2007; Nicholson 1993; Shipman et al. 1984; Thompson 2004; Thurman and Wilmore 1980). Black color results from the combustion of the organic components of collagen and carbon, while gray and white colors are the outcome of continued combustion that alters the crystalline structure (Devlin and Herrmann 2013). Table 7 summarizes the different colors seen on bones and associated structural changes. 18 Regarding methods for standardizing color recording, the Munsell Soil Color Chart has been used extensively (Kharkanis et al. 2009; McCutcheon 1992; Munro et al. 2007), following Shipman et al. (1984). Researchers have more recently started investigating the use of the RGB (Red-Green-Blue) colour space and CIE *L*a*B* colour space (a model defined by the Commission Internationale de l’Eclairage, with values for L*: lightness, a*: red-green values, b*: yellow-blue values, which locates color on a three-dimensional axis), together with digital photography and spectrophotometers to enhance objectivity and statistical processing (Devlin and Herrmann 2015; Krap et al. 2019; Ulguim 2015; Walker et al. 2008). Table 8 summarises the strengths and weakeness of these methods. Table 7. Color change to burned bone and causative factors (adapted from Fairgrieve 2008 Table 3.5 and references therein) Colour Causative factors Brown Hemoglobin, soil discoloration Black Bone carbonization Gray-blue, gray Pyrolysis of organic components White Bone calcination Table 8. Advantages and disadvantages of commonly used color recording systems (Thompson et al. 2017 Table 21.2) Color recording system Strengths Weaknesses Munsell • Standardised • • • • RGB • • • • Standardised Objective Quantifiable Easily comparable • Requires post-processing or expensive equipment CIE L*a*B* • • • • Standardised Objective Quantifiable Easily comparable • Requires expensive equipment Subjective Difficult to capture surface color variation Developed for soil color analysis Difficult for statistical manipulation Depending on the research questions, different recent studies have adopted schemes of coding bone color changes with different levels of detail. Weitzel and McKenzie (2015) simply classified skeletons into two groups: those that exhibited charring (color mostly black) and those that also showed small spots of dark grey, grey and white (colors representing calcination). The location of charring was recorded for each skeletal element and then for the skeleton as a whole, whereby categories were created based on the number of skeletal elements affected by fire as a percentage of the total number of elements present (100%, 75–99%, 50–74%, 25–49% and 0–24% charred). Curtin (2015) adopted a slightly more detailed recording scheme: unburned (no apparent heat-related changes), slight burning (light brown or reddish discoloration, often localised), moderate burning (more extensive dark brown or black discoloration), and severe burning (calcined bone, white, grey or blue-grey in color, often warped and shrunken). Alternative schemes include the categories ‘unburned’ (no change in color), ‘lightly burned’ (brown or black, including ‘smoked’ fragments) or ‘heavily burned’ (blue-grey or white, also known as ‘calcined’) (Bontrager and Nawrocki 2015). Color is usually recorded per element or per fragment (Lara et al. 2015). Either all colors visible on each element/fragment or the dominant color may be recorded (Watson et al. 2015). 19 Fleshed versus dry bone The issue of distinguishing the pre-incineration state of bones (dry versus fleshed) has been examined by several authors. This issue has implications for both forensic anthropology and bioarchaeological studies, as it provides information about the postmortem treatment of a body/individual. Warping and thumbnail fractures (curved transverse fractures) are among the main attributes adopted in relevant studies. Baby (1954) noted that green (recently defleshed) bone demonstrates warping, whereas dry bone shows no warping but it manifests patina, longitudinal, and transverse fractures. Binford (1963) reported dry bones as having straight transverse cracking, while green bones had curved transverse cracking. Stewart’s (1979) findings agreed with those by Binford. However, contrary to the curved transverse fractures noted by Binford (1963), Thurman and Willmore (1980) found that fleshed cremations are characterised by serrated transverse fractures. Etxeberria (1994) found warping occurring only on recently defleshed bones, but not on dry bones. In contrast, Spennemann and Colley (1989) identified warping on an experimentally burned archaeological humerus. This finding was later corroborated by Buikstra and Swegle (1989) who, based on their study of human and animal bones, reported warping in both green and dry bones burned in open-air fires. Similarly, Whyte (2001) found that warping affected fleshed, recently defleshed and dry animal bones that had been experimentally burned. More recently, Gonçalves et al. (2011) found warping in dry human bones burned at a crematorium, lending further support to the fact that this kind of deformity is not exclusively linked to the burning of fleshed or recently defleshed bones. As discussed in Mayne Correia (1997), the disagreement of the results of previous studies can largely be attributed to differences in the experimental methods and the type of skeletal materials adopted, as well as to inconsistencies in terminology. More recent experimental work by Gonçalves et al. (2015b) explored the frequency of heat-induced warping and thumbnail fractures on cremations of cadavers and skeletons. The authors confirmed that heat-induced warping and thumbnail fractures may occur in dry bones, though they are much more frequent in cadavers (fleshed bodies) than in skeletons. In addition, in burned skeletons, thumbnail fractures were almost thrice as frequent as warping. Therefore, warping is a better indicator of the pre-burning condition of remains. In addition to the above patterns, remains burned in a wet/fleshed state usually exhibit varying color patterns, deep fracturing and delamination, whereas bones burned in a dry state usually show little color variation, superficial fracturing and limited delamination (Bontrager and Nawrocki 2015). More recently, Lemmers et al. (2020) suggested that evidence of bioerosion lesions can still be identified histologically in burned remains and these can offer important insights to the pre-burning treatment of the body, that is, they can indicate whether the remains had been buried prior to being exposed to heat or not (but see cautionary note by Végh et al. 2021 who discovered that fire may actually produce features that resemble bioerosion). Weights During heat exposure, bone mass is reduced due to moisture loss, fat combustion and the breakdown/oxidation of organic molecules (Grupe and Hummel 1991; Thompson 2004, 2005). Several investigations experimentally addressed bone mass reduction using different animal bones burned for different durations and at different temperatures (e.g. Enzo et al. 2007; Grupe and Hummel 1991; Hiller et al. 2003; Munro et al. 2007; Person et al. 1996). Some studies have shown a bone mass loss of 10% up to 200oC, 30% beyond 300oC, and 40% beyond 900oC (Bonucci and Graziani 1975; Grupe and Hummel 1991). Skeletal weight should always be recorded on burned remains and may often be the only workable data in very fragmentary material. This parameter may allow for some insights especially regarding the completeness of the remains, the degree of anatomical identification and the proportions of each skeletal region (Gonçalves 2011). Relevant studies are largely based on the work of Lowrance and Latimer (1957), who weighed adult skeletons of Asian origin after the bones had been macerated and degreased. They were able to determine the percentage of mass each bone contributed to the total mass of the skeleton. When an anatomical region is underrepresented, it raises issues of selective bone treatment or preservation (André et al. 2013). Trotter and Peterson (1962) studied the relationship between 20 skeletal mass and the mass of ash. The skeletons used by the authors were macerated, degreased and dried before being heated to 600oC. The mass lost varied between 30 and 39% depending on the bone (Trotter and Peterson 1962). André et al. (2013) applied Trotter and Peterson’s percentages (1962) to Lowrance and Latimer’s (1957) data, and calculated new proportions of each bone and each anatomical region (Table 9). Table 9. Calculation of the theoretical proportions of bones and anatomical regions for a skeleton burned to 600oC from Trotter and Peterson’s data (1962) applied to Lowrance and Latimer’s data (1957) (André et al. 2013 Table 2) Percentages of bone weights (Lowrance and Latimer 1957) Head Trunk Bone weights for a skeleton of 2882 g Percentages of bone weights after burning to 600oC (Trotter and Peterson 1962) Bone weights after burning to 600oC Percentages of bone weights for a skeleton of 2882 g burned to 600oC Cranium 17.98 518.18 67.01 347.22 18.19 Mandible 2.42 69.74 69.71 48.62 2.55 Vertebrae 10.06 289.93 63.96 185.45 9.71 Ribs 6.42 185.02 64.95 120.17 6.29 Sternum 0.47 13.55 62.87 8.52 0.45 2.84 81.85 65.84 53.89 2.82 1.04 29.97 66.01 19.78 1.04 Humerus 6.38 183.87 66.89 122.99 6.44 Ulna 2.66 76.66 67.69 51.89 2.72 Radius 2.18 62.83 67.59 42.46 2.22 Hand bones 2.53 72.91 66.54 48.51 2.54 7.83 225.66 64.64 145.87 7.64 17.67 509.25 66.69 339.63 17.79 Patella 0.57 16.43 66.2 10.87 0.57 Tibia 10.63 306.36 66.92 205.01 10.74 Fibula 2.47 71.19 67.64 48.15 2.52 Foot bones 5.79 166.87 66 110.13 5.77 Upper Scapula limbs Clavicle Lower Hip bones limbs Femur Percentages of bone weights for each anatomical region (Lowrance and Latimer 1957) Percentages of bone weights for each anatomical region for a skeleton burned to 600oC 20.4 20.7 17 16.5 17.6 17.8 45 45 21 Rather than estimating the weight of individual burned bones or anatomical regions, some researchers have documented it at the full skeleton level in order to assess the completeness of assemblages involving burned human skeletal remains (McKinley 1993; Warren and Maples 1997). The weight of burned remains can be compared to these reference standards to assess their completeness. Such documentation has already been carried out for several populations in Europe (Gonçalves et al. 2013b; Herrmann 1976; Malinowski and Porawski 1969; McKinley 1993), the United States (Bass and Jantz 2004; Van Deest et al. 2011; Warren and Maples 1997) and Asia (Chirachariyavej et al. 2006). Representative data are given in Tables 10-11. The mean skeletal weight of the burned skeletons reported in all those studies presented great variation, which may be the result of different approaches adopted in weighing the remains, or linked to age, sex and regional differences (Bass and Jantz 2004; Chirachariyavej et al. 2006; May 2011; McKinley 1993; McKinley and Bond 2001; Van Deest et al. 2011). Indeed, more aged individuals showed lower weights, and females systematically weighed less than males (Bass and Jantz 2004; Chirachariyavej et al. 2006; Malinowski and Porawski 1969; May 2011). With regard to regional differences, Bass and Jantz (2004) and May (2011) attributed these to regional variation in obesity rates and the body weight of different living populations. Chirachariyavej et al. (2006) further highlighted that different coffins may lead to variation regarding the weight of burned remains since often the coffin ashes are weighted along with the skeletal remains. Table 10. Mean weight (in grams) for burned skeletal remains per sex (Gonçalves 2011 Table 1.1.2) Females Males Reference 1540 2004 Malinowski and Porawski (1969) 1700 1842 Herrmann (1976) 1616 2284 McKinley (1993) 1840 2893 Warren and Maples (1997) 2350 3379 Bass and Jantz (2004) 2120 2680 Chirachariyavej et al. (2006) 2238 3233 Van Deest et al. (2011) Table 11. Average weights of burned remains (in grams) in males and females from different archaeological periods (Minozzi 2015 Table 17.3) Assemblage Females Males Reference Ligurian Apuans 862 1173 Minozzi (2015) Borgo Panigale 592 812 Cavazzuti (2008/2010) Pisa 1061 1255 Bagnoli (2011/2012) Casinalbo 656 974 Cavazzuti (2008/2010) German Urn field culture 438 562 Wahl (2015) German Hallstat/La Tenè 401 572 Wahl (2015) 22 Additionally to using burned remains’ weights to assess the completeness of an assemblage, this method has been adopted to estimate the minimum number of individuals and the sex of an individual (Bass and Jantz 2004; Warren and Maples 1997), although these approaches have serious weaknesses (Fairgrieve 2008; McKinley and Bond 2001). See sections Minimum Number of Individuals and Sex assessment. Recording the weight of burned remains Despite the limitations associated with the use of burned remains weight, it is important to collect relevant information, at least as a means of quantifying the available material and its preservation (see section Sorting and Cataloguing). For this purpose, the weight of bone per sieve fraction should be obtained, as well as the total weight of bone from the combined sieve fractions. The weight from each sieve fraction should be represented as a percentage of the total weight. Weight in grams should be measured to one decimal place. Another application of skeletal weights is related to the reconstruction of the funerary practices of past populations. Reference weights have been used to assess how thoroughly burned remains were collected prior to their deposition in the urn or grave (Gonçalves et al. 2010, 2015b; Holck 1986; McKinley 1994a, 1994b; Murad 1998; Murray and Rose 1993; Richier 2005; Smits 1998). However, this approach does not take into account that not all parts of the skeleton are equally affected by heat-induced weight loss and that many bone fragments exposed to heat can no longer be anatomically identified (Gonçalves 2011). A general serious limitation of using published weight standards is that such standards have been estimated based on calcined adult (often rather aged) individuals that were burned in modern crematoria. Thus, comparisons with non-calcined, non-adult remains, or remains burned in open pyres is problematic (Thompson et al. 2017). Bone microstructure As already stressed, many of the above presented macroscopic changes are associated with microstructural alterations due to heat exposure. Some broad such alterations are presented in Table 12. Table 12. Heat-induced bone microstructural alterations (Carroll and Squires 2020) Category Temperature I 100oC-400oC Thin-section micrograph Description well preserved bone microstructure; circular well-formed Haversian systems; unaltered Volkmann’s canals, osteons and canaliculi 23 Category Temperature II 500oC-600oC Thin-section micrograph Description depletion of organic material; fusion of hydroxyapatite crystals; microfeatures still identifiable, but less well preserved III 700oC-900oC more visible degeneration of microscopic features; increased hydroxyapatite fusion; decomposition of all organic material IV >1000oC complete hydroxyapatite fusion → no discernible osteons, Volkmann’s canals and canaliculi; few misshaped Haversian systems 24 Crystallinity Index Heat-related variation of the crystallinity index is an important topic examined with regard to the effect of heat on bone mineral structure. When bone is fresh, its crystal structure is poorly ordered, contains small crystals and has a greater strain; unburned bone, therefore, has a low crystallinity index value (Paschalis et al. 1997; Thompson et al. 2011). When exposed to heating, the crystal structure of bone becomes better ordered and is characterised by larger crystals and less porosity and strain; hence, the crystallinity index value increases (Bartsiokas and Middleton 1992; Figueiredo et al. 2010; Munro et al. 2007; Nagy et al. 2008; Olsen et al. 2008; Shipman et al. 1984; Stiner et al. 2001; Surovell and Stiner 2001; Trueman et al. 2008). Representative Crystallinity indices calculated from XRD (CI) and FTIR (IRSF) data in bones incinerated for 150 mins are given in Table 13. Table 13. Crystallinity indices at different temperatures calculated from XRD (CI) and FTIR (IRSF) data (drawn from Greiner et al. 2019 Table 3) Temperature (oC) CI IRSF Unheated 0.11 2.43 100 0.14 2.67 200 0.16 2.81 300 0.17 2.97 400 0.19 3.12 500 0.24 3.56 600 0.35 4.26 700 1.23 6.17 800 1.31 6.32 900 1.28 6.29 1000 1.30 6.96 Regarding the factors underlying the increase in crystallinity index values, Rogers and Daniels (2002) argued that their data support an increase in crystal size as causative factor, but they also stated that a redistribution of existing crystals may also play a role. Hiller et al. (2003) agreed that crystal size indeed increases with heat exposure, but crystal shape and thickness also alter. Person et al. (1996) and, more recently, Sui et al. (2014) stressed the importance of the organic phase in protecting the inorganic phase from change and, therefore, its influence on crystallinity index - although this will only occur up until the loss of the organic phase. Trueman et al. (2008) and Lebon et al. (2010) agreed with these findings and explained them more specifically by highlighting the role of the organic phase in reducing bone porosity, thus crystal surface area exposure to the environment. Heat-induced changes in bone crystallinity (Etok et al. 2007) y 25–250oC: loss of poorly bounded water up to 100oC and of structural water from proteins and mineral surface-bound water up to 250oC y 300–500oC: combustion of about half of the organic phase, increase of crystal size (from ca. 10 to 30 nm) and crystallite thickness (from ca. 2 to 8-9 nm), and formation of new mineral phases y > 500oC: loss of remaining organic matter, crystallite size enlargement to 110 nm and crystallite thickness growth to 10 nm y 900oC: loss of intercrystallite space y > 900oC: formation of β-tricalcium phosphate 25 Different methods have been used to examine heat-induced bone crystallinity changes: x-ray diffraction (XRD), small-angle x-ray scattering (SAXS), Fourier transform infrared spectrocopy (FTIR), Raman spectroscopy, and others (Mamede et al. 2017; Munro et al. 2007; Sui et al. 2014; Thompson et al. 2009). Crystallinity index values from these methods are not directly comparable, although the general trends they reveal are similar. They all have advantages, but FTIR is preferable because it allows the simultaneous examination of all bone tissue components, it can identify contaminant material and detect substitutions within the elemental structure, it is cost-efficient and more accurate at lower heating temperatures (D’Elia et al. 2007; Paschalis et al. 1997; Wright and Schwarcz 1996). Other scholars advocate XRD over FTIR (Pijoan et al. 2007; Pucéat et al. 2004; Rogers et al. 2010), while others still have used the two approaches in combination (Munro et al. 2007; Pucéat et al. 2004). Beyond being heat-induced, crystallinity changes also occur naturally after death and are enhanced by weathering (Brock et al. 2010; Piga et al. 2009; Stiner et al. 1995, 2001; Surovell and Stiner 2001). Rogers et al. (2010) examined differences in the microstructural changes that occur in diagenetically altered and burned bones to test whether the two processes can be differentiated. Indeed, the authors identified differences in the lattice order of the mineral of these types of bone, as well as in the directional nature of their microstructure. When examining crystallinity, it should be remembered that it is not uniform throughout the skeleton (Nakano et al. 2002; Thompson et al. 2009), while age has also been shown to affect it, with younger bone showing lower values than more mature bone (Paschalis et al. 1997). However, Thompson et al. (2011) highlighted the importance of extrinsic variables (e.g., temperature and duration of heat exposure) over intrinsic ones (e.g., skeletal sample location). Moreover, there is evidence that certain pathological conditions can also affect crystallinity index values. Nagy et al. (2008) found a difference in crystallinity index between archaeological remains afflicted by tuberculosis and syphilis. Wright and Schwarcz (1996) also noted that dense enthesophytic bone may be responsible for an unexpected crystallinity index value in their work. Finally, the fire context is also important. Heat from a pyre generally comes from one direction and will cause greater heat-induced crystallinity index changes on the bone surface closest to it (Schurr et al. 2015). Before closing, it must be noted that with appropriate carbonate compensation, increases in crystallinity will still produce a constant crystallinity index (Pucéat et al. 2004). DNA analysis A number of studies have highlighted the potential to extract DNA from burned remains once these have been exposed to temperatures up to 600oC (sometimes 300-400oC), at which point much of the organic component is oxidised (Brown et al. 1995; Duffy et al. 1991; Harbeck et al 2011; McKinley 2017; Pusch et al. 2000; Sajantila et al. 1991; Tsuchimochi et al. 2002; Williams et al. 2004). In cases of bone exposure to higher temperatures and/or for long duration, such as in modern commercially cremated remains, experimental and case studies indicate DNA cannot be retrieved (Cattaneo et al. 1999; Nelson and Melton 2007; Rees and Cox 2010; von Wurmb-Schwark et al. 2004). Stable and radiogenic isotope analysis Among the earliest experimental studies on the applicability of stable isotope analysis on burned skeletal remains, DeNiro et al. (1985) found that boiling or roasting did not change the δ13C and δ15N values by more than 1‰; however, more extreme heating altered δ13C and δ15N values by as much as 5‰ and 4‰, respectively. More recently, Schurr et al. (2015) confirmed that stable nitrogen isotope ratios are significantly affected by heating; however, contrary to DeNiro et al. (1985), δ13C values did not change particularly by heating, although in burned samples they showed increased scatter around the mean value of −13.2 ± 0.1‰. For oxygen isotopes, Munro et al. (2007) found that δ18Op values were preserved at temperatures lower than 300°C; however, at higher temperatures, values were up to 7‰ smaller. In their experimental work, Harbeck et al. (2011) concluded that stable isotopic values of the light elements carbon, nitrogen and oxygen are unaltered up to 200oC, but over this temperature, results are unreliable. These results disagree with a recent experimental study by Robinson and Kingston (2020) who tested the effect of heating on faunal δ13Cenamel and δ18Oenamel and found that although δ18O values are significantly altered from 300oC onwards, δ13C values are minimally affected at temperatures as high as 1100oC (except in thin-enameled species). Stable isotopic values of the heavy element strontium also remain unchanged up to 1000oC, as supported in experimental studies (Snoeck et al. 2015) and case studies using archaeological material (Graham and Bethard 2019; 26 Snoeck et al. 2016, 2018; Taylor et al. 2020). In this direction, the work by Harvig et al. (2014) showed that strontium isotope ratios estimated from the otic capsule of the petrous part of the temporal bone show a high correlation with ratios obtained from the dental enamel of the same individuals, in burned and unburned remains. Hence, the petrous bone can substitute dental enamel where the latter is too fragmented. The validity of radiocarbon dating of burned bones has been supported by different studies (Minami et al. 2019; De Mulder et al. 2007, 2012; Naysmith et al. 2007; Olsen et al. 2008, 2011). However, in several cases charred bones exhibit aberrant results, likely due to post-depositional CO3 substitution (Olsen et al. 2008; Van Strydonck et al. 2009). Contrary to charred remains, in calcined bones, the compactness of the bone structure prevents carbonate substitution (Van Strydonck et al. 2005). Thus, dating should employ calcined bone rather than charred one (Lanting et al. 2001). A serious potential limitation in radiocarbon dating is the so-called ‘old wood effect’, that is, we may be dating the wood used for the cremation pyre rather than the skeletal remains (Geyh 2001; Olsen et al. 2013; Rose et al. 2020; Snoeck et al. 2014; Van Strydonck et al. 2010; Zazzo et al. 2012). Heat-induced dental alterations As with bone, many extrinsic variables control the effect of heat on teeth, such as temperature, duration of exposure, state of the body before exposure, and others. Nonetheless, some broad patterns can be outlined. During fire exposure, different teeth and dental tissues are differentially affected. The anterior teeth may be less affected compared to the posterior teeth if they fall out at an early stage of the burning process; once they are on the ground, they are likely below or away from the fire. However, if they do not fall out, the enamel of the anterior teeth is more affected by heat exposure than that of the molars because the latter are better protected by the orofacial tissues (Delattre 2000; Sakoda et al. 2000). Posterior teeth are generally rather protected and their crown fragmentation is more predictable than that of anterior teeth, with cracks appearing first along the grooves that separate the cusps (Schmidt 2015). An early but seminal study on the effect of heat on dental tissues was by Harsányi (1976). The key findings regarding heat-induced macroscopic and microscopic alterations to enamel and dentin are presented in Tables 14-15. Shipman et al. (1984) also systematically explored microscopic heat-induced dental changes, as summarised in Tables 16-17. Table 14. Macroscopic and microscopic effects on enamel of one-hour heat exposure at different temperatures (drawn from Harsányi 1976; reprinted by Fairgrieve 2008 Table 7.2) o C Macroscopic changes Microscopic changes 200 Color changes None 300 Dark grayish brown color; enamel starts to peel off via small crevices Small crevices; enamel intact between crevices 500 Gray color; longitudinal furrows Crevice network; multiangular plates 700 Light grayish-white color; fragmentation Fine grained granules; original surface unrecognizable 900 Almost white color; more pronounced fragmentation Fusion of enamel grains; unrecognizable structure 1000 Porcelain-white color “Structureless” smooth plates 1100 Porcelain-white color; fragmentation Same as at 1000oC 1300 Tiny smooth porcelain-white fragments with glass-like surface Inorganic salts fused into round formations 27 Table 15. Macroscopic and microscopic effects on dentin of one-hour heat exposure at different temperatures (drawn from Harsányi 1976; reprinted by Fairgrieve 2008 Table 7.5) o C Macroscopic changes Microscopic changes 200 Color changes None 300 Light grayish-brown color Structure preserved; tubules opened horizontally or longitudinally; morphology unaffected 500 Dark grayish-black color; pulp chamber and root canal preserved Preserved-open dental canalicules, no narrowing 700 Pale gray color; parts of pulp chamber and root canal recognizable but narrowed Tubules narrowed but visible; peritubular zone heat-resistant relative to intertubular dentin 900 Almost white color; large pieces with root present Narrowed dentin tubules 1.5 to 1.7 μm in diameter; anastomoses between tubules not visible 1000 Porcelain-white color; narrowed pulp chamber; root canal slightly distinguishable Tubular structure preserved; minute “pearls” of material in string formation 1100 Porcelain-white root color; narrowed pulp chamber and root canal still observable Tubular structure preserved; narrow portions and anastomoses not observable; round plates and granules of varying size 1300 Minute porcelain-white fragments; remains of narrowed pulp chamber and root canal may be observable Structures decomposed and fused into granules of varying size Table 16. Microscopic heat-induced alterations to enamel (drawn from Shipman et al. 1984; reprinted by Fairgrieve 2008 Table 7.3) Stage I 20-185°C: enamel normal Stage II 185-285°C: dimples develop, but overall surface texture smoother than in Stage I Stage III 285-440oC: rounded particles form and cover the surface Stage IV 440-800°C: vitrified or glassy particles separated by pores and fissures; enamel close to the CEJ breaks up Stage V 800-940°C: fine particles of stage IV coalesce into larger, smooth-surfaced globules that fuse into an irregularly-shaped mass pierced by rounded holes Table 17. Microscopic heat-induced alterations to dentin (drawn from Shipman et al. 1984; reprinted by Fairgrieve 2008 Table 7.6) Stage I 20-185°C: dentinal surface of pulp cavity normal; calcospherites clearly visible and pierced by smooth-edged, circular openings to the dentinal tubules Stage II 185-285°C: peritubular matrix shrunken and separated from intertubular matrix; surface of intertubular matrix showing small asperities that produce roughened texture Stage III 285-440°C: asperities of stage II have melted and smoothed out; division between peritubular and intertubular matrix rarely visible; elongated openings of dentinal tubules; intertubular matrix forming a network of bars between openings Stage IV 440-800°C: appearance of many particles which create frothy or fleecy texture; increasing elongation and enlargement of tubule openings; some areas of glassy texture, perforated by irregularly-shaped openings Stage V 800-940oC: frothy protuberances of stage IV have coalesced into globules that fuse into nodular spikes; spaces between spikes are remnants of tubules and spikes are remnants of intertubular bars 28 A series of recent experimental studies by Sandholzer and his colleagues (Sandholzer 2015; Sandholzer et al. 2013, 2014a, 2014b) complemented these earlier works and found that the enamel was fully preserved and attached to the dentin at 400°C, with small cracks visible in the crown and multiple small cracks in the root. Between 500°C and 700°C the enamel was partially separated from the coronal dentin or fragmented; deep cracks were present in the root, and small cracks on the enamel surface, with the majority appearing in the dentin-enamel junction. At temperatures above 800°C, the enamel and dentin were separated and fragmented. Recent studies have also examined systematically color changes in the tooth crown and root (Figure 7). Changes in the enamel are subtle, from natural pale yellow color to pale brown, light grey and white (Beach et al. 2015). Heatrelated color changes in roots go from pale yellow to black, brown, greyish-blue, light grey, chalky-white and, finally, white-pink (Beach et al. 2015; Fairgrieve 2008; Sandholzer et al. 2013). It must be noted that the part of the root that lies inside the socket during heat exposure is better protected, and its color will often differ from that of the exposed part of the root (Schmidt 2015). Figure 7. Color alteration of human teeth after 30-minute heat exposure at 400oC (A) to 1000oC (G) (adapted from Sandholzer 2015 Figure 21.1) Important dimensional changes also take place during dental exposure to heat, as is the case with bones. Sandholzer (2015) and Sandholzer et al. (2013) found mean dentinal shrinkage in tooth roots to be between 4.78% (at 400°C) and 32.53% (at 1000°C), showing a sharp increase between 700°C and 800°C (11.5–24.2%) (Table 18). Similarly, Beach et al. (2015) examined the degree of weight loss at different temperatures and for different durations of exposure, and the results are summarised in Table 19. Table 18. Mean dentinal volume shrinkage at different temperatures after constant 30-minute exposure (Sandholzer et al. 2013 Table 1) Temperature (oC) Shrinkage (% ± 1 SD) 400 4.78 ± 0.80 500 5.94 ± 1.36 600 8.66 ± 0.83 700 11.53 ± 1.53 800 24.20 ± 4.23 900 27.50 ± 4.35 1000 32.53 ± 5.35 29 Table 19. Average percentage weight loss for teeth at different temperatures after constant 30- and 60-minute exposure (Beach et al. 2015 Table 7.2) Temperature (oC) Average percentage weight loss at 30 min Average percentage weight loss at 60 min 204 16.3 13.1 260 17.3 17.3 316 13.3 23.1 371 16.9 21.3 427 37.9 22.5 482 25.1 30.9 538 27.4 24.1 593 36.0 33.3 The stage of development of a tooth needs to be taken into consideration when examining the effect of fire. Silva et al. (2009) found that in teeth with more than two thirds of the crown formed, a clear distinction may be seen between the forming enamel that has higher organic content, and more completely mineralised enamel. Similarly, it is important to consider if teeth are deciduous or permanent. Most of the abovementioned research examined permanent teeth. An important study focused on the deciduous dentition is by Karkhanis et al. (2009); the results are summarized in Table 20 and partly visualized in Figure 8. Table 20. Heat-induced changes in deciduous teeth (Karkhanis et al. 2009 Table 1) o C Color Stereomicroscopic and SEM traits 100 • Crown: pale yellow, very pale brown • Root: yellowish brown • Crown fracturing in anterior teeth • Surface crazing • Melting of external surface 200 • Crown: pale yellow, gray • Cervical patches: very dark brown • Root: shiny black • Surface bubbling and vesicle formation on root surface • Silvery deposits on root surface 300 • Crown: light gray • Patches: very dark gray • Root: shiny black • Surface bubbling on root surface • Globular knob-like formations on predentinal surface 400 • • • • Enamel: very pale brown Patches: very dark brown Dentin: very dark gray Cementum: light yellowish brown • Initial separation of enamel and dentin • Deep fissures on root surface and through dentin 500 • • • • Enamel: light gray Patches: dark gray Dentin: dark bluish gray Cementum: grayish brown, light grayish brown • • • • 600 • • • • Enamel: light gray Cervical patches: very dark gray Dentin: dark bluish gray Cementum: bluish black • Extreme fragility; deep fissures in dentin and cementum 30 Crown-root separation Complete separation of enamel and dentin Loss of enamel lustre Reduction in tubular diameter, especially near dentino-enamel junction o C Color Stereomicroscopic and SEM traits 700 • Enamel: light bluish gray • Dentin: dark bluish gray • Cementum: light bluish black • Extreme reduction in dentinal tubule diameter 800 • Enamel: bluish gray • Dentin: very dark bluish gray • Cementum: very dark bluish gray • Specular appearance in predentinal surface 900 • • • • Enamel: neutral white Dentin: light bluish gray Cementum: light bluish gray Patches: very dark bluish gray • Star-shaped fibrillar structures emerging from intertubular dentin matrix • Cementum unidentified, with granular appearance 1000 • • • • Enamel: light bluish gray Dentin: light bluish gray Cementum (external): bluish black Cementum (subsurface): light bluish gray • Obliteration of dentinal tubules • Identifiable prismatic structure of enamel and tubular morphology of dentin 1100 • Enamel/dentin/cementum: light bluish gray • Predentin: light greenish gray, pink discoloration of the crown • Identifiable enamel and dentin • Granular appearance of intertubular dentin • Cementum unidentifiable, with granular appearance Figure 8. 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Instead, try to fit the information given below in a spreadsheet (e.g. in Excel) whereby each individual element occupies a row and each variable is given in a column. GENERAL INFORMATION Archaeological site: Curation site: Recorder: Date: Burial No: Grave type: Grave size: Field methods for site identification: Field methods for site excavation: Cleaning methods: Restoration methods: 47 BONE INVENTORY Key: Zones as defined by Knüsel and Outram (2004); record expression per zone as 0 = absent, 1 = present <25%, 2 = present 26-50%, 3 = present 51-75%, 4 = present >76% or simply as 0 = absent, 1 = present CRANIUM, MANDIBLE, EAR OSSICLES & HYOID Element Zone/Side Frontal Element Zone/Side 1 Vomer – 2 Lacrimal 3 Palatine 4 Ethmoid – Occipital 5 Mandible 1 Temporal 6 2 7 3 8 4 9 5 10 6 11 7 Parietal Sphenoid Zygomatic Maxilla Nasal Inferior nasal concha 48 Expression 12 Malleus 13 Stapes 14 Incus 15 Hyoid – Expression THORACIC CAGE & VERTEBRAE Element Zone Sternum 1 Rib 1 Left Right Rib 11 1 3 3 1 4 Axis 2 1 3 2 4 C3-7 1 1 2 2 3 3 4 T1-12 1 2 2 3 3 1 4 2 L1-5 3 Expression 1 3 1 Rib 12 Atlas 2 3 Rib 3-10 Zone 2 2 Rib 2 Element 1 2 3 4 SHOULDER GIRDLE Element Zone Clavicle 1 Left Right Element Zone Scapula 1 2 2 3 3 Left Right 4 5 6 7 8 9 49 UPPER AND LOWER LIMB LONG BONES & PATELLA Element Zone Humerus 1 Radius Element Zone Femur 1 2 3 3 4 4 5 5 6 6 7 7 8 8 9 9 10 10 11 11 1 Patella – 2 Tibia 1 3 2 4 3 5 4 6 5 7 6 8 7 9 8 10 9 11 10 Fibula 1 A&B 2 C 3 D 4 E 5 F 6 G H J 50 Right 2 J Ulna Left Left Right HAND BONES Element Zone Left Right Scaphoid – Lunate – 2 Triquetral – 3 Pisiform – Trapezium – 2 Trapezoid – 3 Capitate – Hamate – MC1 1 MC4 1 Proximal phalanx Left Right Left Right 1 1 2 3 Middle phalanx 3 1 1 2 3 2 Distal phalanx 3 MC3 Zone MC5 2 MC2 Element 1 1 2 3 2 3 PELVIC BONES Element Zone Os coxa 1 Left Right Element Zone Sacrum 1 2 2 3 3 4 4 5 6 7 8 9 10 11 12 51 FOOT BONES Element Zone Talus 1 Right Element Zone MT3 1 2 2 3 3 4 Calcaneus Left MT4 1 1 2 2 3 3 MT5 1 4 2 5 3 Navicular – Cuboid – 2 1st Cuneiform – 3 2nd Cuneiform – 3rd Cuneiform – 2 MT1 1 3 2 MT2 Middle phalanx Distal phalanx 1 1 1 3 2 1 3 2 3 52 Proximal phalanx Left Right BROADLY IDENTIFIED BONE Type Size class Cranium <1 cm Thorax Pectoral girdle Upper limb No of fragments Weight Type Size class Pelvic girdle <1 cm 1-3 cm 1-3 cm 3-5 cm 3-5 cm >5cm >5cm <1 cm Lower limb 1-3 cm 3-5 cm 3-5 cm >5cm >5cm Unidentifiable Weight No of fragments Weight <1 cm 1-3 cm <1 cm No of fragments <1 cm 1-3 cm 1-3 cm 3-5 cm 3-5 cm >5cm >5cm <1 cm 1-3 cm 3-5 cm >5cm UNIDENTIFIED BONE Type Size class Flat <1 cm Short No of fragments Weight Type Size class Long epiphysis <1 cm 1-3 cm 1-3 cm 3-5 cm 3-5 cm >5cm >5cm <1 cm Long diaphysis <1 cm 1-3 cm 1-3 cm 3-5 cm 3-5 cm >5cm >5cm 53 DENTAL INVENTORY Key: 1 = Present, not in occlusion, 2 = Present, development completed, in occlusion, 3 = Missing, no associated alveolar bone, 4 = Missing, antemortem loss, 5 = Missing, postmortem loss, 6 = Missing, congenital absence, 7 = Present, damage renders measurement impossible, 8 = Present, unobservable PERMANENT TEETH I1 Maxilla Left Maxilla Right Mandible Left Mandible Right I2 C P3 P4 M1 M2 M3 DECIDUOUS TEETH I1 Maxilla Left Maxilla Right Mandible Left Mandible Right I2 C M1 M2 HEAT-INDUCED ALTERATION Skeletal/ dental fragment 1 Preservation of organics1 Fracturing2 Shrinkage3 Warping4 Discoloration5 Preincineration state6 Crystallinity index Record as ‘carbonization’or ‘calcination’ 2 Record as longitudinal, step, transverse, patina, splintering and delamination, burn line, and curved transverse Record only when clearly visible (e.g. when bilateral asymmetry is noted in elements diferentially exposed to heat); otherwise leave blank 3 4 Record as present/absent 5 Record using Munsell Soil Color Chart or any other available method (e.g. RGB) 6 Record as dry or fleshed; assessed via warping, fracturing, and discoloration 54 SEX ASSESSMENT (ONLY FOR ADULT REMAINS) Key: Record as Female, Probable Female, Ambiguous, Probable Male, Male, Indeterminate Element Trait/Method Sex AGE-AT-DEATH ESTIMATION (FOR NONADULTS) Classify individuals in one of the following categories: fetus = before birth, infant = 0-3 yrs, child = 3-12 yrs, adolescent = 12-20 yrs, nonadult = <18 yrs, indeterminate = unable to estimate age-at-death Element Trait/Method Age-at-death AGE-AT-DEATH ESTIMATION (FOR ADULTS) Classify individuals in one of the following categories: young adult = 20-35 yrs, middle adult = 35-50 yrs, old adult = 50+ yrs, adult = 18+ yrs, indeterminate = unable to estimate age-at-death Element Method Stage Age-at-death 55 PATHOLOGICAL LESIONS Element affected 56 Type of lesion Degree of expression ISBN 978-9963-2858-7-7