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The following kits were used in each reaction: TaqMan[R] Fast Universal PCR Master Mix (Applied Biosystems, USA); SybrGreen PCR Master Mix (Applied Biosystems, USA) and GoTaq[R] qPCR Master Mix Plexor System (Promega, USA).
Different methods of real-time PCR for detection of pseudorabies virus/Diferentes metodos de PCR em tempo real para deteccao do virus da pseudorraiva
The first PCR reaction detected the Aspergillus spp, Fusarium spp, and Scedosporium spp, whereas the second PCR reaction detected the Mucorales: 12.5-[micro]L of 2x PCR master mix (Plexor qPCR system) and a 7.5-[micro]L primer mix was prepared as a 20-[micro]L reaction mix, followed by the addition of 5 [micro]L of DNA sample.
Quantitative Multiplexed Detection of Common Pulmonary Fungal Pathogens by Labeled Primer Polymerase Chain Reaction
The patellar or knee jerk, reflex is tested by stimulating patellar tendon with a plexor or hammer.
Clinical evaluation of methyl predisolone sodium succinate (MPSS) for management of accidental paresis in canines--a report of 6 patients
Novel Plexor SNP genotyping technology: comparisons with TaqMan and homogenous MassEXTEND MALDI-TOF mass spectrometry.
Current methods for fluorescence-based universal sequence-dependent detection of nucleic acids in homogenous assays and clinical applications
O programa utilizado para desenho dos primers foi o Plexor Primer Design (Promega, Wisconsin, USA).
Enzootic bovine leukosis real time PCR/PCR em tempo real para diagnostico da leucose enzootica bovina
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