protoplast

In plants, the efficacy of CRISPR/Cas9 system gene disruption has also been reported by several researchers using transient expression by agroinfiltration and protoplast transformation (Li et al., 2013; Nekrasov et al., 2013; Shan et al., 2013), but in previous studies albino spots or photo-bleaching were not observed clearly using agroinfiltration based transient expression (Li et al., 2013).

As a control, the protoplast of mutant NTG1 was treated by five successive rounds of NTG mutagenesis.

In our preliminary studies, the mentioned decapeptide showed to be active only in protoplast cells, which is an indicator of deep target site for such molecule and role of cell wall as a penetration barrier for this peptide.

Somatic embryogenesis and regeneration of plantlets in protoplast cultures from somatic embryos of coffee (Coffea canephora P.

Bohorova, et al., [112] isolated and cultured protoplasts of wild and cultivated sunflower and obtained roots and meristematic regions from protoplast derived callus.

Plantlet regeneration from protoplasts of Muscari armeniacum Leichtl.

Scanning Electron Microscopy (SEM) and energy dispersive X-ray (EDX) microanalysis were also used by the researcher to analyse plant tissues of the lower epidermis, mesophyll protoplasts, and cell walls.

Developed through the non-GMO process of "protoplast fusion," the new X-series of hybrid yeasts by Erbsloh are a combination of Saccharomyces cerevisiae and a subspecies of bayanus.

Infectivity of MSV has also been abolished due to mutation or deletion of/in CP gene of maize lead to non accumulation of ssDNA particles in protoplast (Boulton et al., 1989, 1993).

Content and vacuole/extravacuole distribution of neutral sugars, free amino acids, and anthocyanins in protoplast. Plant Physiology, 64: 88-93.

They may also protect the protoplast maintaining cellular integrity when subjected to water stress (PAIVA; MACHADO, 2008).

It blew my mind when I combined Bacillus subtilis and Bacillus thermophilus via protoplast fusion and made a new living species for my thesis."