Near the end of the film, George Jetson legs are shown tied up and untied at different moments.
When Lucy 2 says "and Teddy 2," her voice isn't robotic, it's the actress' natural voice.
While the Jetsons are in flight to their new home, Jane is on George's right hand side. Jane then switches places with George to his left side when he kisses him. The next scene she's back to being on his right side.
the scene where Astro and George get on the new treadmill, George asks Astro what speed he wants slow (left,) medium (middle,) fast (right). Astro says "slow". George clearly presses to the right where fast is.
When Mr. Spacely's graph is zig-zagging on the company's profits, the outline of the chart is visible as it moves.
Mr. Spacely unplugs Rudy 2's wiring, shutting him down. Rudy 2 is later functioning with no explanation.
Judy repeatedly refers to Cosmic Cosmo as her boyfriend, but they were never in any kind of relationship. He just randomly selected her from the audience to be his date for an evening, and when Judy had to move, he went with someone else.
In the original series, Elroy was depicted as a boy genius and child prodigy. In the movie, he's portrayed as just an ordinary kid.
During the scene where Astro's tail caught on fire, his finger changes from six fingers, to eight fingers in the same scene.