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Genetic screening

Single-cell CRISPR screening in mouse brain

Nature Protocols (2025)Cite this article

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Single-cell CRISPR screening is a powerful method for biological discovery. Such screens genetically perturb many genes in parallel and use single-cell RNA sequencing to assess the transcriptomic effects. Now, an in vivo protocol facilitates screens in mouse brain, using adeno-associated virus with transposon-based delivery of the CRISPR guide RNAs.

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Fig. 1: Protocol overview for single-cell CRISPR screening in mouse brain.

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Authors and Affiliations

  1. CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria

    Eugenia V. Pankevich & Christoph Bock

  2. Medical University of Vienna, Institute of Artificial Intelligence, Center for Medical Data Science, Vienna, Austria

    Christoph Bock

Authors
  1. Eugenia V. Pankevich
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  2. Christoph Bock
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Correspondence to Christoph Bock.

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Competing interests

C.B. is a co-founder and scientific advisor of Myllia Biotechnology and Neurolentech. E.V.P. declares no competing interests.

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Pankevich, E.V., Bock, C. Single-cell CRISPR screening in mouse brain. Nat Protoc (2025). https://doi.org/10.1038/s41596-024-01128-2

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