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    DNA Synthesis

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    LifescienceBiosyn
    DNA Synthesis: Bio-Synthesis world’s leading Supplier providing commercial Synthetic DNA, custom DNA synthesis services and developer of new technologies for unmodified DNA synthesis. For More Visit On- http://www.biosyn.com/dna-synthesis.aspx

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    DNA Synthesis

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    LifescienceBiosyn
    46 views2 pages
    DNA Synthesis: Bio-Synthesis world’s leading Supplier providing commercial Synthetic DNA, custom DNA synthesis services and developer of new technologies for unmodified DNA synthesis. For More Visit On- http://www.biosyn.com/dna-synthesis.aspx

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    DNA Synthesis: Bio-Synthesis world’s leading Supplier providing commercial Synthetic DNA, custom DNA synthesis services and developer of new technologies for unmodified DNA synthesis. For More Visit On- http://www.biosyn.com/dna-synthesis.aspx

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    © All Rights Reserved
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    Download as doc, pdf, or txt
    46 views2 pages

    DNA Synthesis

    Uploaded by

    LifescienceBiosyn
    DNA Synthesis: Bio-Synthesis world’s leading Supplier providing commercial Synthetic DNA, custom DNA synthesis services and developer of new technologies for unmodified DNA synthesis. For More Visit On- http://www.biosyn.com/dna-synthesis.aspx

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    A brief understanding of DNA synthesis

    DNA synthesis (Deoxyribonucleic acid) is a process by which duplicates or copies of


    nucleic acid tresses are made. Naturally, DNA synthesis takes place inside cells by a
    natural mechanism which is called as DNA replication. Using genetic engineering
    and enzyme chemistry, scientists have developed man-made methods to synthesize
    DNA. Poly-merase chain reaction or PCR is the most important step in DNA
    synthesis. It was first originated in early 1980, PCR since then become a multi-billion
    dollar industry. The original patent was being sold for 300 million dollars.
    The discovery of DNA was done in 1951 by Francis Crick, James Watson, and
    Maurice Wilkins. Using x-ray crystallography data generated by Rosalind Franklin,
    Watson and Crick determined that the structure of DNA was that of a double helix.
    For this work, Watson, Crick, and Wilkins received the Nobel Prize in Physiology or
    Medicine in 1962.
    Since DNA synthesis is very important pertaining diagnosis and medical
    investigations. The key to understand DNA synthesis is knowing its structure first.
    DNA is a long chain of polymer made up of chemical units called nucleotides. This is
    also called a genetic material. It is the DNA molecules which carry information that
    dictates protein synthesis in most living organisms. Typically, DNA exists as two
    chains of chemically linked nucleotides. These links follow specific patterns dictated
    by the base pairing rules. Each nucleotide is made up of a deoxyribose sugar
    molecule, a phosphate group, and one of four nitrogen containing bases. The bases
    include the pyrimidines thymine (T) and cytosine (C) and the purines adenine (A)
    and guanine (G). In DNA, adenine generally links with thymine and guanine with
    cytosine. The molecule is arranged in a structure called a double helix which can be
    imagined by picturing a twisted ladder or spiral staircase. The bases make up the
    rungs of the ladder while the sugar and phosphate portions make up the ladder
    sides. Nucleotides are linked in an order which is termed as the sequence, is
    determined by a process known as DNA sequencing.

    In a eukaryotic cell, DNA synthesis occurs just before the cell division in a course
    called replication. When replication begins the two strands of DNA are separated by
    a variety of enzymes. Thus opened, each strand serves as a template for producing
    new strands. This whole process is catalyzed by DNA polymerase. This molecule,
    the DNA polymerase, brings corresponding or complementary nucleotides in line
    with each of the DNA strands. Then the nucleotides are chemically linked to form
    new DNA strands which are exactly a copy of the original strand. These copies,
    called the daughter strands, contain half of the parent DNA molecule and half of a
    whole new molecule. Replication by this method is known as semi conservative
    replication. The process of replication is an important one because through this
    method only the cell transfers the exact duplicate of their genetic material from one
    generation of cell to the next.
    Controlled DNA synthesis starts by identifying a small segment of DNA to copy. This
    is characteristically a specific sequence of DNA that holds the code for a desired
    protein.
    Called template DNA, this material is needed in concentrations of about 0.1-1 micrograms. This calls for higher purification because even trance amount of the
    compounds used during DNA purification restrains the PCR process. One method for
    purification of DNA strands is treating it with 70% of ethanol.

    For More Visit on http://www.biosyn.com/dna-synthesis.aspx

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