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    HPLC Column

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    rama
    An HPLC column contains a stationary phase that differentially interacts with compounds in a mobile phase, separating the compounds as they flow through. A chromatogram records the separation process over time, producing a graph where the retention time and peak area indicate characteristics like a compound's concentration. Retention times of target compounds and their concentrations per peak area are determined in advance using reference standards.

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    HPLC Column

    Uploaded by

    rama
    15 views1 page
    An HPLC column contains a stationary phase that differentially interacts with compounds in a mobile phase, separating the compounds as they flow through. A chromatogram records the separation process over time, producing a graph where the retention time and peak area indicate characteristics like a compound's concentration. Retention times of target compounds and their concentrations per peak area are determined in advance using reference standards.

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    An HPLC column contains a stationary phase that differentially interacts with compounds in a mobile phase, separating the compounds as they flow through. A chromatogram records the separation process over time, producing a graph where the retention time and peak area indicate characteristics like a compound's concentration. Retention times of target compounds and their concentrations per peak area are determined in advance using reference standards.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    15 views1 page

    HPLC Column

    Uploaded by

    rama
    An HPLC column contains a stationary phase that differentially interacts with compounds in a mobile phase, separating the compounds as they flow through. A chromatogram records the separation process over time, producing a graph where the retention time and peak area indicate characteristics like a compound's concentration. Retention times of target compounds and their concentrations per peak area are determined in advance using reference standards.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 1

    HPLC column:

    A tube which contains the stationary phase differentially interacts with the samples constituent
    compounds as they are carried along in the mobile phase.

    Chromatogram:
    When the separation process is monitored by the recorder starting at the time the sample is injected,
    a graph is obtained. This is called a chromatogram. The time required for a compound to elute (called
    retention time) and the relationship between compound concentration (amount) and the peak area
    depend on the characteristics of the compound. Retention time is therefore used as an index for
    quantitative determination. The retention time of the target compounds and the concentration for each
    unit of peak area are based on data obtained in advance by analyzing a sample with known quantities
    of the reference standards.

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