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    Evaluation of Caffeine Aspartame and Sugar

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    This document summarizes a study that evaluated the levels of caffeine, aspartame, and sugar in 10 brands of energy drinks using HPLC and UV spectrophotometric procedures. The study found that caffeine levels ranged from 1.11-237.95 ppm, aspartame ranged from 283.84-956.82 ppm, and sugar ranged from 91.05-1686.73 ppm. All levels were below FDA standards. The study concluded that consumption of the energy drinks is safe unless consumed in large excessive quantities but that excess sugar intake can increase health risks.

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    Evaluation of Caffeine Aspartame and Sugar

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    This document summarizes a study that evaluated the levels of caffeine, aspartame, and sugar in 10 brands of energy drinks using HPLC and UV spectrophotometric procedures. The study found that caffeine levels ranged from 1.11-237.95 ppm, aspartame ranged from 283.84-956.82 ppm, and sugar ranged from 91.05-1686.73 ppm. All levels were below FDA standards. The study concluded that consumption of the energy drinks is safe unless consumed in large excessive quantities but that excess sugar intake can increase health risks.

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    This document summarizes a study that evaluated the levels of caffeine, aspartame, and sugar in 10 brands of energy drinks using HPLC and UV spectrophotometric procedures. The study found that caffeine levels ranged from 1.11-237.95 ppm, aspartame ranged from 283.84-956.82 ppm, and sugar ranged from 91.05-1686.73 ppm. All levels were below FDA standards. The study concluded that consumption of the energy drinks is safe unless consumed in large excessive quantities but that excess sugar intake can increase health risks.

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    Download as pptx, pdf, or txt
    74 views17 pages

    Evaluation of Caffeine Aspartame and Sugar

    Uploaded by

    api-309713761
    This document summarizes a study that evaluated the levels of caffeine, aspartame, and sugar in 10 brands of energy drinks using HPLC and UV spectrophotometric procedures. The study found that caffeine levels ranged from 1.11-237.95 ppm, aspartame ranged from 283.84-956.82 ppm, and sugar ranged from 91.05-1686.73 ppm. All levels were below FDA standards. The study concluded that consumption of the energy drinks is safe unless consumed in large excessive quantities but that excess sugar intake can increase health risks.

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    Evaluation of Caffeine, Aspartame, and Sugar

    Contents in Energy Drinks


    Journal of Chemical and Pharmaceutical Research
    Casimir Gimba, Stephem Abechi, Nurudeen Abbas & Igelige Gerald
    Presented by: Victoria Do

    Outline
    Overview
    Introduction
    Experimental Section
    Data
    Results & Discussion
    Conclusion
    Q & A

    Overview
    Risks and benefits of energy drinks has gained momentum in

    recent times.
    The purpose of this study is to determine the level of caffeine,

    aspartame, and sugar in energy drinks


    The study was evaluated by using HPLC (high-performance liquid

    chromatography) and UV spectrophotometric procedures

    Introduction
    Energy drinks contain caffeine as an active ingredient:
    A central nervous system stimulant
    Increase epinephrine also known as adrenaline
    Effect speed at which information is process
    Reduce Sleep
    Enhance:
    Mood
    Alertness
    Awareness
    Attention
    Reaction

    time

    Introduction
    Aspartame is a low calorie sweetener used in energy

    drinks
    200 times sweeter than sugar
    Enhances and extends flavors
    A synthetic non nutritive sweetener
    Provides a far lower net calorie intake

    Introduction
    Sugar content in energy drinks
    A carbohydrate
    Sweeteners to improve taste
    Ranges from 21g 34g per 8 oz
    Comes in the from of sucrose, glucose, or high fructose

    corn syrup

    Experimental Section
    10 brands of energy drinks samples were randomly

    selected
    Levels of caffeine, aspartame, and sugar were
    determined,
    All reagents were analytical or HPLC grade
    All solutions were prepared by using distilled water

    Sample Preparations
    Samples were diluted with distilled water
    Filtrated
    Transferred to vials and into auto sample tray
    Injected into HPLC (High performance liquid

    chromatography) column

    Sample Preparation cont.


    The concentration was determine in energy drink for each

    dilute sample of:


    Caffeine
    Aspartame
    Samples concentration was calculated from a calibration
    curve
    Chromatographic analysis is carried out at wavelengths

    Determination of Sugar
    All 10 samples were placed in a spectrometer
    A blank solution is used first to read the absorbance
    Recall from in class lab procedure Spectrophotometric

    Analysis of Caffeine and Benzoic Acid


    Absorbance for diluted samples was recorded

    Data

    Table 1: Concentration of Sugar, Caffeine and Aspartame

    a-k: Increasing order of sugar and aspartame. a-j: Increasing order of caffeine.
    Values numbers with the same letters in the column, by treatment, are not significantly different while those with
    different letters are significantly different at p 0.05% from each other.

    Results and Discussion


    Caffeine Concentration
    Ranged from 1.11 ppm 237.95 ppm
    Sample EV had the least concentration (1.11 ppm)
    Sample PH had the highest concentration (237.95 ppm)

    Lower than the ranged of caffeine concentration in:


    Energy drinks reported in literature
    Tea sample reported in literature

    All energy samples were below the FDA set standards

    Results and Discussion


    Aspartame Concentration
    Ranged from 283.84 ppm 956.82 ppm
    Sample WD had the least concentration (283.84 ppm)
    Sample EV had the most concentration (956.82 ppm)

    Lower than the ranged of caffeine concentration in:


    Fruit juice
    Powdered drinks

    Aspartame concentrations in all energy samples were below the

    FDA set standard

    Results and Discussion


    Sugar Concentration
    Ranged from 91.05 ppm 1686.73 ppm
    Sample JW had the least concentration (91.05 ppm)
    Sample LB had the high concentration (1686.73 ppm)

    Excess consumption of sugar can lead to:


    Dental caries
    Oral hygiene
    Over weight
    cardiovascular disease

    Conclusion
    Caffeine consumption can have health consequences,

    particularly at high dose.


    The concentration of caffeine and aspartame in the
    energy drinks were observed to be lower than the
    acceptance daily intake set by the WHO/FDA.
    All energy samples will not have adverse effects on the
    consumers except multiples of the drinks are consumed.

    Future Studies
    Safety level of caffeine consumption with lack of

    sleep amongst college students.

    Reference
    Gimba, C. E., Abechi, S. E., Abbas, N. S., & Gerald, I. U.

    (2014). Evaluation of caffeine, aspartame and sugar


    contents in energy drinks. Journal Of Chemical &
    Pharmaceutical Research, 6(8), 39-43.

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