Candidate

Centre Number Number

Candidate Name

CAMBRIDGE INTERNATIONAL EXAMINATIONS

General Certificate of Education Advanced Level

BIOLOGY 9700/5
PAPER 5 Practical Test
OCTOBER/NOVEMBER SESSION 2002
1 hour 30 minutes
Candidates answer on the question paper.
Additional materials:
As listed in Instructions to Supervisors

TIME 1 hour 30 minutes

INSTRUCTIONS TO CANDIDATES
Write your name, Centre number and candidate number in the spaces at the top of this page.
Answer both questions.
Write your answers in the spaces provided on the question paper.

INFORMATION FOR CANDIDATES

The intended number of marks is given in brackets [ ] at the end of each question or part question.
You are advised to spend 40 minutes on Question 1 and 50 minutes on Question 2.

FOR EXAMINER’S USE

TOTAL

This question paper consists of 6 printed pages, 1 blank page and a Report Form.
SP (SC/KN) S35158/2
© CIE 2002 [Turn over
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Examiner’s
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Question 1 [40 minutes]

K1 is a stained, longitudinal section of a young root tip in which some cells are undergoing
mitosis.

Examine K1 carefully, in the region labelled X in Fig. 1.1, using low- and high-power objectives of
your microscope.

Y Z

root cap

Fig. 1.1

(a) (i) Make a labelled, high-power drawing of a cell in interphase from region X.

[4]

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(ii) Make a labelled, high-power drawing of two cells showing different stages of
mitosis from region X.

[6]
(iii) Examine carefully the cells from the central region of the line labelled Y-Z.

Draw one cell from this region and annotate your drawing to indicate how it differs
from the cells you drew in (ii).

[4]

[Total : 14]

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Question 2 [50 minutes]

You have been provided with three germinated pea seeds, labelled S4, and a solution of
hydrogen peroxide, labelled S5.

Germinating peas produce the enzyme catalase. The enzyme catalyses the following reaction.

2H2O2 → 2H2O + O2

Carefully remove the whole length of the shoot from one of the pea seedlings and place it in a
beaker. Cover the shoot with distilled water and gently boil the shoot for three minutes.
Remove the shoot and place it on a white tile. Add a spatula full of sand.
Use a glass rod and ensure the shoot is well macerated (crushed).
Place the macerated tissue in a test-tube and label it S6.

Wash and blot dry the glass rod and the tile.

Carefully remove the shoots from the second and third pea seedlings. Do not boil these shoots,
but place them on the tile, add a spatula full of sand to each and carefully squash each shoot
separately with the glass rod. Place each fresh, macerated shoot in separate test-tubes, labelled
S7 and S9.

Place a spatula full of sand in a test-tube and label it S8.

(a) Put 2 cm3 of hydrogen peroxide in a measuring cylinder and pour it into test-tube S6.

Record your observations in Table 2.1.

Table 2.1

observations

S6

S7

S8
[1]

Repeat this procedure for S7 and S8.

(b) Put 1 cm3 of hydrogen peroxide and 1 cm3 of distilled water in a measuring cylinder and
add this to S9.

Record your observations in Table 2.2.

Table 2.2

observations

S9
[1]

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(c) Compare your observations of S6, S7, S8 and S9 and explain them.

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(d) Explain three ways by which you could improve the experimental design.

1. ......................................................................................................................................

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2. ......................................................................................................................................

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3. ......................................................................................................................................

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Question 2 continues on the next page.

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An experiment was carried out to determine the uptake of oxygen, using germinating peas
placed in a respirometer, as shown in Fig. 2.1.

syringe

screw-clip
scale

respiration
chamber
compensation
tube
filter paper wick

water
strong KOH
solution
manometer

Fig. 2.1

(e) Describe the function of the compensation tube in the respirometer.

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......................................................................................................................................[2]

(f) Explain the procedures that you would follow to determine the rate of oxygen uptake by
the germinating peas in the respirometer.

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......................................................................................................................................[3]

(g) Suggest and explain how the respirometer could be modified to determine the
respiratory quotient (RQ) of the germinating peas.

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......................................................................................................................................[2]

[Total : 16]

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BLANK PAGE

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REPORT FORM

The teacher responsible for this subject is asked to answer the following questions.

(a) Was the candidate physically handicapped in drawing or in using a microscope or is the
candidate colourblind? If so, give brief details.

(b) Was the candidate handicapped by deficient material or apparatus? If so, give brief details.

(c) Was it necessary to make any substitutions for the materials sent from Cambridge? If so, give
brief details of the circumstances.

(d) Any comments.

Signed ………………………………

N.B. Information that applies to all candidates need only be given on the first candidate’s
answer book

9700/5/O/N/02