UH5300
UH5300
UH5300
Simple
Easy
The technology for spectrophotometers continues to advance.... Hitachi designs spectrophotometers encompassing technologies for the future.
-Providing the best-in-its-class levels of performance and unprecedented operability*1-
*1 : Survey by Hitachi High-Technologies Corporation. Models offered for sale in the Japanese market as of August, 2012 *2 : Windows PCs can be used to control the UH5300. *3 : Use of a Windows PC allows wired connection. For further information on system conguration, see page 13.
Feature
Use of long-life xenon ash lamp
Smart
Light source is guaranteed for seven years*4, resulting in lower operation cost
Double-beam optical system
Ensures extended periods of data stability compared with the single-beam optical system
Operation by use of tablet terminals
Simple
Easy
*4 : Registration of the product is required. For further information, please ask your dealer. The lamp is guaranteed against failure for seven years after the date of delivery or for a specied number of lamp illuminations, whichever comes rst. The specied number of illuminations is represented as a percentage (%), depending on the working conditions of the lamps, in the maintenance menu of the UH5300, in reference to a lamp working condition of 100%. Assuming that the spectrophotometer is used for seven years, 240 days a year, the lamp working condition of 100% corresponds to 300 measurement events of absorbance measurement per day (number of wavelengths : one wavelength) or 20 measurement events per day for of wavelength scan measurement per day (scan range : 190 to 1,100 nm, scan speed : 200 nm/min).
Performance
Hitachi has achieved the best-in-its-class*1 performance spectrophotometer using a combined xenon flash lamp and a stable double beam optical system. This level of performance allows for highly accurate data to be obtained
Xenon flash lamps as long-life light sources - Hitachi UH5300 guarantees the light sources for seven years*4-
Long Life
Unlike conventional systems*5, the use of xenon flash lamps reduces the frequency of lamp replacement. Xenon flash lamps of the Hitachi UH5300 are guaranteed for 7 years*4.
Less heat emission from the lamps reduces the need for stabilization of the lamps unlike conventional devices. Due to its environmentally conscious design, the UH5300 consumes 25% less electricity than conventional devices*5.
Best-of-class levels*1 of resolution of 1 nm have been achieved. -A uniquely designed wavelength drive system achieves a good balance between high-speed scanning and high wavelength accuracy-
High Resolution
Best-of-class*1 resolution at 1 nm has been achieved. The European Pharmacopoeia specifies, as a standard of the resolution, an absorbance ratio of 1.5 or higher at the designated peaks of 0.02% (V/V)
toluene solution. The absorbance ratio of the UH5300 is 2.04, surpassing the standard, as shown in the figure below.
Using a certified reference material such as holmium filter enables to check the wavelength accuracy using the peak detection function
Abs 0.5 0.4 0.3 0.2 0.1 0.0 265 266 Abs 0.5 0.4 0.3 1.0 0.2 267, 0.221707 0.1 0.0 nm 274 267 265 268 266 269 267 270 268 271 269 272 270 273 271 274 272 275 273 275 267, 0.221707 0.5 0.5 1.0 268.7, 0.451797 268.7, 0.451797 Abs 2.0 Abs 2.0
1.5
1.5
0.0 nm 250
0.0 300 250 350 300 400 350 450 400 500 450 550 500 600 nm 550 600 nm
Stable optical system by using double beam - Allows extended periods of stable measurements-
High Stability
Light dispersed through a monochromator is divided into two luminous fluxes by a half mirror. The drift of the system is reduced by comparing the photometric values of the sample light and the reference light.
Absorbance (Abs)
0.005 0.004 0.003 0.002 0.001 0 -0.001 -0.002 -0.003 -0.004 -0.005
500
1000
2000
2500
3000
3500
Absorbance (Abs)
Features
Due to its symmetrical optical system, deviations in light intensity are corrected with high accuracy.
A N
0.005 0.004 0.003 0.002 0.001 0 -0.001 -0.002 -0.003 -0.004 -0.005 0 500 1000 1500 Time (s) 2000 2500 3000 3500
Comparison of baseline stability Single-beam device (above) Double-beam device (below : UH5300)
M4
M1
Diffraction grating
M6
M5
Sample cell
M2
M3
High-throughput measurement by using the 6-cell turret -the Intelligent Start function makes it easier to use the deviceG T
High Throughput
H
Intelligence
The Intelligent Start function provides superior operability and results in a reduction of measurement time. J Place a sample cell in the sample chamber and close the sample W chamber door to 6 trigger an automatic measurement * . The 6-cell turret, when used in auto start mode, may reduce measurement time.
K
*6 : Patent pending (Japanese Patent Application No. 2012-181302)
to Auero Z
Place samples
Operation
Instrument control through a wireless LAN connection Hitachi offers a new type of operation in laboratories.
Printer
Printing
Data files are stored in the internal memory. The use of a wireless LAN connection allows instrument operators, data processors, system administrators, and various other users to have access to the instrument regardless of their location. The data acquisition status or data from measurements can also be checked while way from the instrument, providing a sense of security.
Remote Control
Multi Access
Remote Control
Remote control via wireless LAN allows the user to check the data in real time while away from the instrument. This is especially useful when running multiple samples or a time scan using an extended period of time.
Multi Access
Routers, if used, allow the connection of more than one tablet terminal or PC*7. Instrument control and access to data can be achieved through control terminal devices, while access to the data stored in the device can be made through other connected terminals.
Data Retention/Printing
Data Transfer
Measurement data are stored in the internal memory of the UH5300. The data can be transferred to a USB memory drive through the USB ports. It can also be transferred to storage devices on the network.
AirPrint
An AirPrint-compatible printer, if used, allows a direct print over a wireless LAN from a tablet terminal.
Functions
Advanced touch screen user interface Resulting in intuitive operation
Pinch Zoom in
Touch Panel
Flick
Provides touch operation, pinch-zoom-in/out operation, and flick operation that are unique to touch panels. In the spectrum display screen, for example, pinch-zoom-in operation contracts a graphic image, while pinch-zoom-out operation expands it. Flick operation on the 6-cell turret on the screen also allows interlocking of the control software screen and cell movement.
Tap!!
A,a,1,2,3
Characters can be input by using the touch keyboard displayed on a tablet terminal, and numeric characters can be input by using the slider bar. Provides flexible input operations, both by mouse-click operation on a PC and tap operation on a tablet terminal.
Visual Icon
Visual icons of universal design make the operation simple. Even novice operators will find it intuitive and easy to use the instrument.
Examples of icon designs
Software
Simple and easy-to-use control software Expand measurement possibilities just by touching the screen.
Main functions of application software -Main MenuThe main menu provides access to various functions. The control software is designed with emphasis on simple and intuitive operability, providing an easy-to-follow measurement flow, even for someone who is unfamiliar with a spectrophotometer.
Measurement
Various measurement modes are available, including concentration, wavelength scan, and time change measurement. For details, see page 8.
Maintenance
Mode for performance check and wavelength calibration. Lamp conditions can also be checked.
Method File
Used to locate method les by specifying specic information such as analyst, sample name, etc.
Top screen (Main menu)
Basic Settings
Basic Settings of the spectrophotometer Used to specify a network destination for auto-saving les as well as the selection of display colors for graphs.
Data le
Used to locate data les by specifying measurement information such as analyst, sample name, etc.
Simplied operational ow
Setting of analysis parameters
Select a measurement mode and set the analysis parameters
Start of measurement
Measurement starts after baseline correction and automatic zero setting
Data analysis
Measurement results are checked. Secondary processing of data is available.
Save
Measurement screen
CSV conversion
Measurement can be accomplished in three steps: analysis parameters setting, measurement, and data processing. Measurement data are saved in the internal memory of the UH5300. The stored data can also be printed out via commercially available printers or converted into the CSV le format for storage in the USB memory, PC, or external storage devices on networks.
Measurement Menu
Various measurement modes are available to accommodate a wide range of applications.
Concentration measurement
Measures standard solutions to prepare a calibration curve and uses it to perform quantitative analysis of unknown samples. Also, provides quantitative determination using entering coefficients.
Absorbance/Transmittance measurement
Used for continuous measurement of single-wavelength or multiple-wavelength absorbance (transmittance). Allows selection of up to 6 wavelengths.
Monitor measurement
This mode is a useful mode for the measurement of either absorbance or transmittance at a single wavelength while reading the results.
*8 : To browse from an iPad, the free iBooks application, which is available from Apple, is required.
Application
The UH5300 supports various measurement applications.
Environmental Field
Quantitative determination of hexavalent chromium (diphenylcarbazide - absorption photometric method)
Shown on the right is an example of quantitative determination of hexavalent chromium using the diphenylcarbazide - absorption photometric method. Plating by trivalent chromate treatment used to comply with the RoHS Directive can be analyzed for the presence of hexavalent chromium.
Abs Abs
0.6 0.6 0.5 0.5 0.4 0.4 0.3 0.3 0.2 0.2 0.1 0.1 0.0 0.0 400 400 450 450 500 500 550 550 600 600 650 650 700 nm 700
nm
Abs
Abs
0.8 0.8 0.7 0.7 0.6 0.6 0.5 0.5 0.4 0.4 0.3 0.3 0.2 0.2 0.1 0.1 0.0 0.0 0.0 0.0
A2 A1 A0 R R2
0.0000 A2 0.0000 0.6648 A1 0.6648 0.0044 A0 0.0044 0.9999 R 0.9999 0.9997 R2 0.9997
0.5 0.5mg/L
mg/L
Foods Industry
Quantitative determination of D-glucose (enzyme method)
D-glucose is widely found in plants and animals and is an essential component in metabolic pathways. An example of measurement by the enzyme method using F-kit* 9 is shown. Making use of an enzyme reaction, coenzyme NADH or NADPH is measured for absorbance at 340 nm to determine an increase or decrease.
1.1 1.1 1.0 1.0 0.9 0.9 0.8 0.8 0.7 0.7 0.6 0.6 0.5 0.5 0.4 0.4 0.3 0.3 0.2 0.2 0.1 0.1 0.0 0.0 -0.1 -0.1 0 0 50 50 100 100 150 150 200 200
Abs Abs
0.8 0.8 0.7 0.7 0.6 0.6 0.5 0.5 0.4 0.4 0.3 0.3 0.2 0.2 0.1 0.1 0.0 0.0 -0.1 -0.1 -0.2 -0.2 0
A2 A1 A0 R R2
0.0000 A2 0.0000 0.0037 A1 0.0037 0.0168 A0 0.0168 0.9975 R 0.9975 0.9950 R2 0.9950
Bio-Technology Field
Quantitative determination of salmon testicle DNA ( ultraviolet absorption method )
The absorption spectrum of a n u cl e i c a c i d s o l u t i o n s h o w s a minimum absorption at around 230 nm and a maximum absorption at around 260 nm. Use of the nucleic acid measurement mode allows for determination of purity from the two-wavelength ratio as well as determination of the DNA concentration from absorbance at 260 nm.
1.2 1.1 1.0 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0.0 250 300 350
Example of purity and nucleic acid concentration determination using the nucleic acid measurement mode
0.15
0.10
0.05
0.00 250 260 270 280 290 300 310 320 330 340 350
nm
10
Option
A wide range of accessories is available to support various applications.
Accessories
The UH5300 offers a variety of accessories to meet your analytical needs. These accessories support your measurement effort for a wide range of applications.
Micro cell
Micro cells are used in the sample chamber of the UH5300 in combination with the holder base (3J1-0109), single cell holder (3J1-0106), and micro cell mask (200-1537). It is suited for the measurement of a small amount of sample of about 340 to 600 L.
Product name Micro quartz cell, 10 mm Black quartz micro cell, 10 mm Mask for micro cell P/N 124-0357 200-0551 200-1537 Capacity (L) 340 to 600 L Light path length 10 mm
11
Electronic thermostatted cell holder (P/N 131-0301 for 115V) (P/N 131-0302 for 200V)
Electronically controlled constant-temperature cell holder using a Peltier device. This accessory requires front panel ( P/N: 3J1-3214 ) . Place an order separately.
Temperature range Temperature accuracy Temperature stability 0 to 100C (can be set in increments of 0.1C)
(normal temperature : 25C)
within 0.5C*
12
System
Choosing system configurations -Available systems-
LAN connection
The UH5300 is equipped with LAN ports that not only provide a wireless connection through wireless LAN routers but also direct the connection via LAN cables (cross type) to other equipment. Use of a router provides flexible building systems to suit customers requirements.
(a) An example of a system conguration under the tablet terminal control (customer-supplied items)
Contents Fourth generation iPad installed iOS 6 (as of May 2013*10) (Activate (or authenticate) your iPad in your Internet environment before using it.) Wi-Fi (802.11a/b/g/n) -compatible router Category 5e or higher cable (not required if it is supplied with a wireless LAN router). For connection to iPad : AirPrint-compatible printer
13
*10 : Please ask your dealer the latest compatible version and revision of iOS for the UH5300.
Performance check
Simple and easy-to-understand performance check feature is available. Assures the accuracy of data.
Specications
Optical system Wavelength range Spectral band width Stray light Wavelength accuracy Wavelength repeatability Photometric range Photometric accuracy (certied according to NIST SRM 930) Photometric repeatability (repeatability according to NIST SRM 930) Scan speed Response Baseline stability Noise level Baseline atness Light source Detector Cell holder Printer output Interface Size (main Unit) Weight Power Power consumption Ambient temperature Ambient humidity Czerny-Turner mount, Double beam monochromator 190 to 1,100 nm 1 nm 198 nm (KCI) : 1.0 % or less 220 nm (Nal) : 0.05 % or less 340 nm (NaNO2) : 0.05 % or less 0.3 nm (Xe : 260.6, 484.3, 881.9 nm, Hg : 253.7, 435.8, 546.1 nm) 0.1 nm Abs : -3.3 to 3.3 %T : 0 to 300 0.002 Abs (0 to 0.5 Abs) 0.004 Abs (0.5 to 1.0 Abs) 0.002 Abs (0 to 1.0 Abs) 10, 40, 100, 200, 400, 800, 1,200, 2,400, 4,800, 6,000 nm/min Fast, Medium, Slow 0.0005 Abs/h (260 nm after a two-hour warm-up period) 0.0001 Abs (RMS, 260 nm, 0 Abs) 0.0009 Abs (200 to 950 nm) Xenon ash lamp Silicon photodiode Automatic 6-cell turretSingle cell holder are available as an option. For connection to iPad : AirPrint-compatible printer For connection to PC : PC-compatible printer LAN port : connected to a router or PC USB port : connected to USB memory for use when transferring CSV-format les 510 (W) 490 (D) 280 (H) mm 19 kg 100, 115, 220, 230, 240V, 50/60 Hz, 150VA 70 W or Less 15 to 35 C 25 to 80 % (no condensation, to be 70 % or less at temperatures exceeding 30 C)
Software functions
Basic functions
6-cell mode setting function Automatic monitor power-off time setting function Intelligent start function
Measurement modes
Concentration measurement Absorbance/transmittance measurement Nucleic acid measurement (nucleic acid purity, nucleic acid concentration, protein concentration calculation) Wavelength scan Time change Monitor measurement
Wavelength calibration Lamp usage time monitoring Sample chamber opening/closing detection Performance evaluation Performance evaluation using mercury lamps Maintenance history storage
Scale changing Trace Statistical calculation Spectrum overwriting Peak detection Smoothing Differentiation Area calculating Rate calculating
Output capability
Operation software is built into the UH5300. iPad, AirPrint, iBooks, and Safari are registered trademarks or trademarks of Apple Inc. in the U.S. and other countries. For information on AirPrint-compatible devices, see the Apple website. The latest iBook and Safari can be downloaded from the Apple website. Microsoft and Windows are registered trademarks or trademarks of Microsoft Corporation in the U.S. and other countries.
14
CAUTION: For correct operation, follow the instruction manual when using the instrument. Specications in this catalog are subject to change with or without notice, as Hitachi High-Technologies Corporation continues to develop the latest technologies and products for our customers. NOTICE: The system is For Research Use Only, and is not intended for any animal or human therapeutic or diagnostic use.