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    Enzyme Action - Pepsin: " A World of Learning"

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    purplemay333
    This document provides information about the enzyme pepsin and a suggested protocol to observe its action on albumin. Pepsin is a digestive enzyme found in many organisms that aids digestion by breaking down proteins into amino acids via hydrolysis. The protocol calls for preparing suspensions of albumin and pepsin, then combining them in test tubes with varying conditions to act as controls. The test mixture with pepsin and acid should show increased clarity within 15 minutes, demonstrating pepsin's protein-degrading activity works best in acidic environments.

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    Enzyme Action - Pepsin: " A World of Learning"

    Uploaded by

    purplemay333
    45 views1 page
    This document provides information about the enzyme pepsin and a suggested protocol to observe its action on albumin. Pepsin is a digestive enzyme found in many organisms that aids digestion by breaking down proteins into amino acids via hydrolysis. The protocol calls for preparing suspensions of albumin and pepsin, then combining them in test tubes with varying conditions to act as controls. The test mixture with pepsin and acid should show increased clarity within 15 minutes, demonstrating pepsin's protein-degrading activity works best in acidic environments.

    Original Description:

    Pepsin Enzyme

    Original Title

    PepsinInfoSheetMC23_2M

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    This document provides information about the enzyme pepsin and a suggested protocol to observe its action on albumin. Pepsin is a digestive enzyme found in many organisms that aids digestion by breaking down proteins into amino acids via hydrolysis. The protocol calls for preparing suspensions of albumin and pepsin, then combining them in test tubes with varying conditions to act as controls. The test mixture with pepsin and acid should show increased clarity within 15 minutes, demonstrating pepsin's protein-degrading activity works best in acidic environments.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    45 views1 page

    Enzyme Action - Pepsin: " A World of Learning"

    Uploaded by

    purplemay333
    This document provides information about the enzyme pepsin and a suggested protocol to observe its action on albumin. Pepsin is a digestive enzyme found in many organisms that aids digestion by breaking down proteins into amino acids via hydrolysis. The protocol calls for preparing suspensions of albumin and pepsin, then combining them in test tubes with varying conditions to act as controls. The test mixture with pepsin and acid should show increased clarity within 15 minutes, demonstrating pepsin's protein-degrading activity works best in acidic environments.

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    © All Rights Reserved
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    1/44 Rushdale St Knoxfield 3180 or PO Box 9227 Scoresby 3179 Victoria Australia

    Phone: +61 3 9753 3896 Fax: +61 3 9753 3959


    Web: www.southernbiological.com Email: sales@southernbiological.com
    A Division of Cogitamus Pty Ltd ACN 097 468 954 ABN 25 838 926 933




    ENZYME ACTION - PEPSIN
    Enzymes are natures catalysts. They speed up reactions to make them useful for
    metabolic pathways. This protocol describes the action of pepsin on albumin, a globular
    protein. Pepsin is a digestive enzyme that is found in many organisms. It comes in many
    different forms, but in every case, its function is to aid digestion by breaking proteins down
    via hydrolysis into their component amino acids.
    Code: MC23.2M
    Name: Pepsin
    Description: Fine powder, off-white in colour.
    Storage: Store at room temperature (15 30
    o
    C).
    Shelf Life: When stored correctly, MC23.2M has a nominal shelf life of 12 months.
    Safety: Enzymes are biologically active proteins and should be handled with care.
    Avoid direct contact or inhalation.
    Suggested Protocol
    Prepare a 1% suspension of albumin flakes (MC41.1). Weigh the required amount of dry
    flakes into a beaker and add a small amount of cold distilled water. Mix into a paste then
    add boiling distilled water in small amounts with stirring to make up to the required
    volume.
    Prepare a 1% solution of pepsin in distilled water at room temperature.
    Line up 4 test tubes numbered 1 to 4 and add 5mL of the albumin suspension to each, then
    add the following:
    No. 1 3 drops* of 2M HCl and 1mL of pepsin solution (test mixture)
    No. 2 3 drops* of 2M HCl and 1mL of distilled water (control for effect of HCl alone)
    No. 3 3 drops of distilled water and 1mL of pepsin solution (control for effect of pepsin
    at high pH)
    No. 4 3 drops plus 1mL of distilled water (primary control)
    * see tip below for a recommendation on the number of drops of acid to use.
    Stand the four test tubes in a beaker of water at 40-50
    o
    C. You should see a noticeable
    increase in the clarity of the test mixture within about 15 minutes.
    Tip
    Being a digestive protease, pepsin works best in an acidic environment. To optimise the
    activity to suit your particular circumstances, we recommend that you run a trial after
    preparing the reagents. Compare the way the reaction proceeds with 3, 4, 5 and 6 drops
    of 2M HCl then choose the number that works best when your students run the experiment.
    Please Note:
    Batch-to-batch variations in substrate composition and enzyme activity can mean that the
    suggested experiment might not work exactly as described in every situation.

    a world of learning

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