Medicines by Design: U.S. Department of Health and Human Services

AT T E N T I O N R E A D E R S
We would like your comments on Medicines By Design.

Please give us your opinion by lling out this postage-paid response card.
1.
Extent to which the publication held your interest
2.
Understandability
3.
Amount and type of information presented
4.
Join our Findings mailing list. For sample issues,

see http://publications.nigms.nih.gov/ndings.
q I would like to receive Findings, a magazine that
profiles two NIGMS-supported scientists, features

brief descriptions of recent clinically relevant
research, and includes a crossword puzzle based
on words used in the articles.
q I would like to receive a free CD-ROM containing
NIGMS science education booklets on topics such as

cell biology, chemistry, genetics, pharmacology, and
structural biology. These booklets are geared toward
a high school and early college audience. Print
copies of the publications can be ordered from
http://www.nigms.nih.gov/publications/classroom
Usefulness and value of such a publication
Medicines By Design
Please comment on whether Medicines By Design helped you

learn more about:
Name
1.
Pharmacology research
2.
What its like to be a scientist
Address
State
3.
The excitement of biomedical research today
Zip Code
National Institutes of Health

National Institute of General Medical Sciences
E-mail (optional)
Phone (optional)
Other Comments:
U.S. DEPARTMENT OF
HEALTH AND HUMAN SERVICES
City
NIH Publication No. 06-474

Reprinted July 2006
http://www.nigms.nih.gov
U.S. DEPARTMENT OF

What Is NIGMS?
Discrimination Prohibited
Accessibility
The National Institute of General Medical Sciences
Under provisions of applicable public laws enacted
This publication can be made available in
(NIGMS) supports basic biomedical research on
by Congress since 1964, no person in the United
formats that are more accessible to people
genes, proteins, and cells. It also funds studies on
States shall, on the grounds of race, color, national
with disabilities. To request this material in a
fundamental processes such as how cells commu
origin, handicap, or age, be excluded from partici-
different format, contact the NIGMS Ofce
nicate, how our bodies use energy, and how we
pation in, be denied the benets of, or be subjected
of Communications and Public Liaison at
respond to medicines. The results of this research
to discrimination under any program or activity
301-496-7301, TDD 301-402-6327; send e-mail
increase our understanding of life and lay the
(or, on the basis of sex, with respect to any educa-
to info@nigms.nih.gov; or write to the ofce at
foundation for advances in the diagnosis, treatment,
tion program or activity) receiving Federal nancial
the following address: 45 Center Drive MSC
and prevention of disease. The Institutes research
assistance. In addition, Executive Order 11141
6200, Bethesda, MD 20892-6200. If you have
training programs produce the next generation of
prohibits discrimination on the basis of age by
questions about this publication, you can use
biomedical scientists, and NIGMS has programs to
contractors and subcontractors in the performance
the same contact information to reach the writer,
encourage minorities underrepresented in biomedical
of Federal contracts, and Executive Order 11246
Alison Davis, Ph.D.
and behavioral science to pursue research careers.
states that no federally funded contractor may
NIGMS supported the research of most of the
discriminate against any employee or applicant
scientists mentioned in this booklet.
for employment because of race, color, religion,

sex, or national origin. Therefore, the programs of
Disclaimer
Trade names have been used throughout this
booklet to illustrate concepts about medicines
that are familiar to readers. The mention of
specic products is not an endorsement of their
use or effectiveness.
the National Institute of General Medical Sciences

must be operated in compliance with these laws
and Executive Orders.
Additional Copies
and Web Links
To order additional copies of Medicines By Design
DEPT OF HEALTH AND HUMAN SERVICES

NATIONAL INSTITUTES OF HEALTH
NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
45 CENTER DR RM 3AN.32 MSC 6200
BETHESDA MD 20892-6200
OFFICIAL BUSINESS
PENALTY FOR PRIVATE USE $300
or other free publications available from

NIGMS, go to http://publications.nigms.nih.gov/
order/classroom.htm or use the contact
information above.
Medicines By Design is available online at

http://publications.nigms.nih.gov/medbydesign.

OFFICE OF COMMUNICATIONS AND PUBLIC LIAISON
Medicines By Design
U.S. DEPARTMENT OF

Reprinted July 2006
Written by Alison Davis, Ph.D., under contracts

263-MD-205019 and 263-MD-212730.
Produced by the Ofce of Communications and Public Liaison
U.S. Department of Health and Human Services
Contents
FO R E W O R D: A V I SI T T O T H E D O C T O R
C H A PTE R 1: A BC S O F P H AR MAC O LO GY
A Drugs Life
Perfect Timing
Fitting In
10
Bench to Bedside: Clinical Pharmacology
13
Pump It Up
14
C H A PTE R 2: BO DY, H E AL T H YS E LF
16
The Body Machine
16
River of Life
18
No Pain, Your Gain
20
Our Immune Army
23
A Closer Look
26
C H A PTE R 3: DR U GS F R O M N AT U R E , T H E N AN D N O W
28
Natures Medicine Cabinet
28
Ocean Medicines
30
Tweaking Nature
33
Is It Chemistry or Genetics?
34
TestingI, II, III
36
C H A PTE R 4: M O L EC U LE S T O ME D I C I N E S
38
Medicine Hunting
38
21st-Century Science
40
Rush Delivery
41
Transportation Dilemmas
43
Act Like a Membrane
44
The G Switch
46
M E DI C I NE S FO R TH E F U T U R E
48
GLO S SARY
50
Foreword: A Visit to the Doctor
May 17, 2050You wake up feeling terrible,
Thats right, your DNA. Researchers predict that

the medicines of the future may not only look and
and you know its time to see a doctor.

In the ofce, the physician looks you over,
work differently than those you take today, but

tomorrows medicines will be tailored to your
genes. In 10 to 20 years, many scientists expect
listens to your symptoms, and prescribes
that genetics the study of how genes inuence

actions, appearance, and healthwill pervade
a drug. But rst, the doctor takes a look

at your DNA.
medical treatment. Today, doctors usually give you

an average dose of a medicine based on your
body size and age. In contrast, future medicines
may match the chemical needs of your body, as
inuenced by your genes. Knowing your unique
genetic make-up could help your doctor prescribe
the right medicine in the right amount, to boost its
effectiveness and minimize possible side effects.
Along with these so-called pharmacogenetic
approaches, many other research directions will
help guide the prescribing of medicines. The
science of pharmacologyunderstanding the
basics of how our bodies react to medicines and
how medicines affect our bodiesis already a
vital part of 21st-century research. Chapter 1,
ABCs of Pharmacology, tracks a medicines
journey through the body and describes different
avenues of pharmacology research today.
Medicines By Design I Foreword 3
Stay tuned for changes in the way you take
delivery, discussed in Chapter 4, Molecules to
medicines and in how medicines are discovered
Medicines, is advancing progress by helping get
and produced. In Chapter 2, Body, Heal Thyself,
drugs to diseased sites and away from healthy cells.
learn how new knowledge about the bodys own
Medicines By Design aims to explain how
molecular machinery is pointing to new drugs. As
scientists unravel the many different ways medicines
scientists understand precisely how cells interact in
work in the body and how this information guides
the body, they can tailor medicines to patch gaps
the hunt for drugs of the future. Pharmacology
in cell communication pathways or halt signaling
is a broad discipline encompassing every aspect
circuits that are stuck on, as in cancer.
of the study of drugs, including their discovery
Scientists are developing methods to have
and development and the testing of their action
animals and plants manufacture custom-made
in the body. Much of the most promising
medicines and vaccines. Experimental chickens
pharmacological research going on at universities
are laying medicine-containing eggs. Researchers
across the country is sponsored by the National
are engineering tobacco plants to produce new
Institute of General Medical Sciences (NIGMS),
cancer treatments. Topics in Chapter 3, Drugs
a component of the National Institutes of Health
From Nature, Then and Now, will bring you up
(NIH), U.S. Department of Health and Human
to speed on how scientists are looking to nature
Services. Working at the crossroads of chemistry,
for a treasure trove of information and resources
genetics, cell biology, physiology, and engineering,
to manufacture drugs.
pharmacologists are ghting disease in the laboratory
Advances in understanding the roots of disease

are leading to new ways to package tomorrows
medicines. Along with biology and chemistry, the
engineering and computer sciences are leading us
to novel ways of getting drugs where they need
to go in the body. Cutting-edge research in drug
and at the bedside.
CHAPTER 1
ABCs of Pharmacology
now why some peoples stomachs burn after
medicines affect the body. Pharmacology is often
they swallow an aspirin tablet? Or why a
confused with pharmacy, a separate discipline in
swig of grapefruit juice with breakfast can raise
the health sciences that deals with preparing and
blood levels of some medicines in certain people?
dispensing medicines.
Understanding some of the basics of the science
For thousands of years, people have looked in
of pharmacology will help answer these questions,
nature to nd chemicals to treat their symptoms.
and many more, about your body and the medicines
Ancient healers had little understanding of how
you take.
various elixirs worked their magic, but we know
So, then, whats pharmacology?
much more today. Some pharmacologists study
Despite the elds long, rich history and impor
how our bodies work, while others study the
tance to human health, few people know much
chemical properties of medicines. Others investi
about this biomedical science. One pharmacologist
gate the physical and behavioral effects medicines
joked that when she was asked what she did for a
have on the body. Pharmacology researchers study
living, her reply prompted an unexpected question:
drugs used to treat diseases, as well as drugs of
Isnt farm ecology the study of how livestock
abuse. Since medicines work in so many different
impact the environment?
ways in so many different organs of the body,
Of course, this booklet isnt about livestock or

agriculture. Rather, its about a eld of science that
pharmacology research touches just about every

area of biomedicine.
studies how the body reacts to medicines and how
A Juicy Story
Did you know that, in some people, a single glass
of grapefruit juice can alter levels of drugs used
to treat allergies, heart disease, and infections?
Fifteen years ago, pharmacologists discovered
this grapefruit juice effect by luck, after giving
volunteers grapefruit juice to mask the taste of a
medicine. Nearly a decade later, researchers g
ured out that grapefruit juice affects
medicines by lowering levels of a
drug-metabolizing enzyme, called
CYP3A4, in the intestines.
More recently, Paul B. Watkins of
the University of North Carolina at
Chapel Hill discovered that other juices like Seville
(sour) orange juicebut not regular orange
juicehave the same effect on the bodys handling

of medicines. Each of 10 people who volunteered
for Watkins juice-medicine study took a standard
dose of Plendil (a drug used to treat high blood
pressure) diluted in grapefruit juice, sour orange
juice, or plain orange juice. The researchers meas
ured blood levels of Plendil at various times
afterward. The team observed that both grapefruit
juice and sour orange juice increased blood levels of
Plendil, as if the people had received a higher
dose. Regular orange juice had no effect. Watkins
and his coworkers have found that a chemical com
mon to grapefruit and sour oranges,
dihydroxybergamottin, is likely the molecular cul
prit. Another similar molecule in these fruits,
Medicines By Design I ABCs of Pharmacology 5
Many scientists are drawn to pharmacology
A Drugs Life
because of its direct application to the practice of
How does aspirin zap a headache? What happens
medicine. Pharmacologists study the actions of
after you rub some cortisone cream on a patch of
drugs in the intestinal tract, the brain, the muscles,
poison ivy-induced rash on your arm? How do
and the liverjust a few of the most common
decongestant medicines such as Sudafed dry up
areas where drugs travel during their stay in the
your nasal passages when you have a cold? As
body. Of course, all of our organs are constructed
medicines nd their way to their job sites in the
from cells, and inside all of our cells are genes.
body, hundreds of things happen along the way.
Many pharmacologists study how medicines
One action triggers another, and medicines work
interact with cell parts and genes, which in turn
to either mask a symptom, like a stuffy nose, or
inuences how cells behave. Because pharmacology
x a problem, like a bacterial infection.
touches on such diverse areas, pharmacologists

must be broadly trained in biology, chemistry, and
more applied areas of medicine, such as anatomy
and physiology.
A Model for Success

Turning a molecule into a good medicine is neither
easy nor cheap. The Center for the Study of Drug
Development at Tufts University in Boston esti
mates that it takes over $800 million and a dozen
years to sift a few promising drugs from about
5,000 failures. Of this small handful of candidate
drugs, only one will survive the rigors of clinical
testing and end up on pharmacy shelves.
Thats a huge investment for what may seem
a very small gain and, in part, it explains the high
cost of many prescription drugs. Sometimes, prob
lems do not show up until after a drug reaches
the market and many people begin taking the drug
routinely. These problems range from irritating side
effects, such as a dry mouth or drowsiness, to lifethreatening problems like serious bleeding or blood
clots. The outlook might be brighter if pharmaceutical
scientists could do a better job of predicting how
potential drugs will act in the body (a science called
pharmacodynamics), as well as what side effects the
drugs might cause.
One approach that can help is computer mod

eling of a drugs properties. Computer modeling
can help scientists at pharmaceutical and biotech
nology companies lter out, and abandon early
on, any candidate drugs that are likely to behave
badly in the body. This can save signicant
amounts of time and money.
Computer software can examine the atom-by
atom structure of a molecule and determine
how durable the chemical is likely to be inside
a bodys various chemical neighborhoods. Will
the molecule break down easily? How well will
the small intestines take it in? Does it dissolve
easily in the watery environment of the uids
that course through the human body? Will the
drug be able to penetrate the blood-brain barrier?
Computer tools not only drive up the success
rate for nding candidate drugs, they can also
lead to the development of better medicines
with fewer safety concerns.
Inhaled
Oral
Lung
Heart
Liver
Kidney
Stomach
Intravenous
Intestines
A drugs life in the body.

Medicines taken by mouth
(oral) pass through the liver
before they are absorbed
into the bloodstream. Other
forms of drug administration
bypass the liver, entering the
blood directly.
Intramuscular
Subcutaneous
Drugs enter different layers

of skin via intramuscular,
subcutaneous, or transdermal
delivery methods.
Transdermal
Skin
Scientists have names for the four basic stages
a large amount may be destroyed by metabolic
of a medicines life in the body: absorption, distri
enzymes in the so-called rst-pass effect. Other
bution, metabolism, and excretion. The entire
routes of drug administration bypass the liver,
process is sometimes abbreviated ADME. The rst
entering the bloodstream directly or via the skin
stage is absorption. Medicines can enter the body
or lungs.
in many different ways, and they are absorbed
Once a drug gets absorbed, the next stage is
when they travel from the site of administration
distribution. Most often, the bloodstream carries
into the bodys circulation. A few of the most
medicines throughout the body. During this step,
common ways to administer drugs are oral (swal
side effects can occur when a drug has an effect in
lowing an aspirin tablet), intramuscular (getting a
an organ other than the target organ. For a pain
u shot in an arm muscle), subcutaneous (injecting
reliever, the target organ might be a sore muscle
insulin just under the skin), intravenous (receiving
in the leg; irritation of the stomach could be a
chemotherapy through a vein), or transdermal
side effect. Many factors inuence distribution,
(wearing a skin patch). A drug faces its biggest
such as the presence of protein and fat molecules
hurdles during absorption. Medicines taken
in the blood that can put drug molecules out of
by mouth are shuttled via a special blood vessel
commission by grabbing onto them.
leading from the digestive tract to the liver, where
Drugs destined for the central nervous system
broken down, or metabolized. The breaking down
(the brain and spinal cord) face an enormous
of a drug molecule usually involves two steps that
hurdle: a nearly impenetrable barricade called
take place mostly in the bodys chemical process
the blood-brain barrier. This blockade is built
ing plant, the liver. The liver is a site of continuous
from a tightly woven mesh of capillaries cemented
and frenzied, yet carefully controlled, activity.
together to protect the brain from potentially
Everything that enters the bloodstreamwhether
dangerous substances such as poisons or viruses.
swallowed, injected, inhaled, absorbed through the
Yet pharmacologists have devised various ways
skin, or produced by the body itselfis carried to
to sneak some drugs past this barrier.
this largest internal organ. There, substances are
After a medicine has been distributed through

out the body and has done its job, the drug is
chemically pummeled, twisted, cut apart, stuck

together, and transformed.
Medicines and Your Genes

How you respond to a drug may be quite different
from how your neighbor does. Why is that? Despite
the fact that you might be about the same age and
size, you probably eat different foods, get different
amounts of exercise, and have different medical
histories. But your genes, which are different from
those of anyone else in the world, are really what
make you unique. In part, your genes give you
many obvious things, such as your looks, your
mannerisms, and other characteristics that make
you who you are. Your genes can also affect how
you respond to the medicines you take. Your
genetic code instructs your body how to make
hundreds of thousands of different molecules
called proteins. Some proteins determine hair
color, and some of them are enzymes that process,
or metabolize, food or medicines. Slightly different,
but normal, variations in the human genetic code
can yield proteins that work better or worse when
they are metabolizing many different types of
drugs and other substances. Scientists use the
term pharmacogenetics to describe research on
the link between genes and drug response.
One important group of proteins whose genetic
code varies widely among people are sulfation
enzymes, which perform chemical reactions in

your body to make molecules more water-soluble,
so they can be quickly excreted in the urine.
Sulfation enzymes metabolize many drugs, but
they also work on natural body molecules, such
as estrogen. Differences in the genetic code for
sulfation enzymes can signicantly alter blood
levels of the many different kinds of substances
metabolized by these enzymes. The same genetic
differences may also put some people at risk
for developing certain types of cancers whose
growth is fueled by hormones like estrogen.
Pharmacogeneticist Rebecca Blanchard of Fox
Chase Cancer Center in Philadelphia has discovered
that people of different ethnic backgrounds have
slightly different spellings of the genes that make
sulfation enzymes. Lab tests revealed that sulfation
enzymes manufactured from genes with different
spellings metabolize drugs and estrogens at differ
ent rates. Blanchard and her coworkers are planning
to work with scientists developing new drugs to
include pharmacogenetic testing in the early phases
of screening new medicines.
The biotransformations that take place in the
methods can help track
liver are performed by the bodys busiest proteins,
medicines as they travel
its enzymes. Every one of your cells has a variety
through the body,
of enzymes, drawn from a repertoire of hundreds
scientists usually cannot
of thousands. Each enzyme specializes in a partic
actually see where a drug
ular job. Some break molecules apart, while others
is going. To compensate,
link small molecules into long chains. With drugs,
they often use mathe
the rst step is usually to make the substance
matical models and
easier to get rid of in urine.
precise measures of
Many of the products of enzymatic break
body uids, such as
down, which are called metabolites, are less
blood and urine, to
chemically active than the original molecule.
determine where a drug
For this reason, scientists refer to the liver as a
goes and how much
detoxifying organ. Occasionally, however, drug
of the drug or a break
metabolites can have chemical activities of their
down product remains
ownsometimes as powerful as those of the
after the body processes it. Other sentinels, such
original drug. When prescribing certain drugs,
as blood levels of liver enzymes, can help predict
doctors must take into account these added effects.
how much of a drug is going to be absorbed.
Once liver enzymes are nished working on a
Studying pharmacokinetics also uses chem
medicine, the now-inactive drug undergoes the
istry, since the interactions between drug and
nal stage of its time in the body, excretion, as
body molecules are really just a series of chemical
it exits via the urine or feces.
reactions. Understanding the chemical encounters

between drugs and biological environments, such
Perfect Timing
Pharmacokinetics is an aspect of pharmacology
that deals with the absorption, distribution, and
excretion of drugs. Because they are following drug
actions in the body, researchers who specialize in
pharmacokinetics must also pay attention to an
additional dimension: time.
Pharmacokinetics research uses the tools of
mathematics. Although sophisticated imaging
as the bloodstream and the oily surfaces of cells,

is necessary to predict how much of a drug will
be taken in by the body. This concept, broadly
termed bioavailability, is a critical feature that
chemists and pharmaceutical scientists keep in
mind when designing and packaging medicines.
No matter how well a drug works in a laboratory
simulation, the drug is not useful if it cant make
it to its site of action.
10
Fitting In
of arrows. Bernard discovered that curare causes
While it may seem obvious now, scientists did not
paralysis by blocking chemical signals between
always know that drugs have specic molecular
nerve and muscle cells. His ndings demonstrated
targets in the body. In the mid-1880s, the French
that chemicals can carry messages between nerve
physiologist Claude Bernard made a crucial
cells and other types of cells.
discovery that steered researchers toward under
Since Bernards experiments with curare,
standing this principle. By guring out how a
researchers have discovered many nervous system
chemical called curare works, Bernard pointed
messengers, now called neurotransmitters. These
to the nervous system as a new focus for pharma-
chemical messengers are called agonists, a generic
cology. Curare a plant extract that paralyzes
term pharmacologists use to indicate that a molecule
muscleshad been used for centuries by Native
triggers some sort of response when encountering a
Americans in South America to poison the tips
cell (such as muscle contraction or hormone release).
Nerve cells use a chemical
Nerve Cell
Acetylcholine
Curare
Receptor
Muscle Cell
messenger called acetyl

choline (balls) to tell muscle
cells to contract. Curare (half
circles) paralyzes muscles
by blocking acetylcholine
from attaching to its muscle
cell receptors.
The Right Dose

One of the most important principles of pharma
cology, and of much of research in general, is a
concept called dose-response. Just as the term
implies, this notion refers to the relationship
between some effectlets say, lowering of
blood pressureand the amount of a drug.
Scientists care a lot about dose-response data
because these mathematical relationships signify
that a medicine is working according to a specic
interaction between different molecules in the body.
Sometimes, it takes years to gure out exactly
which molecules are working together, but when
testing a potential medicine, researchers must
rst show that three things are true in an experi
ment. First, if the drug isnt there, you dont get
any effect. In our example, that means no change
in blood pressure. Second, adding more of the
drug (up to a certain point) causes an incremental
change in effect (lower blood pressure with more
drug). Third, taking the drug away (or masking
its action with a molecule that blocks the drug)
means there is no effect. Scientists most often

plot data from dose-response experiments on a
graph. A typical dose-response curve demon
strates the effects of what happens (the vertical
Y-axis) when more and more drug is added to
the experiment (the horizontal X-axis).
One of the rst neurotransmitters identied
in a communication between the outside of the
was acetylcholine, which causes muscle contrac
cell and the inside, which contains all the mini-
tion. Curare works by tricking a cell into thinking
machines that make the cell run. Scientists have
it is acetylcholine. By tting not quite as well,
identied thousands of receptors. Because receptors
but nevertheless ttinginto receiving molecules
have a critical role in controlling the activity of cells,
called receptors on a muscle cell, curare prevents
they are common targets for researchers designing
acetylcholine from attaching and delivering its
new medicines.
message. No acetylcholine means no contraction,

and muscles become paralyzed.
Effect on Body
Y-axis
Response
Desired
Effect
Dose-response curves
determine how much of
a drug (X-axis) causes
a particular effect, or a
side effect, in the body
(Y-axis).
Side
Effect
Dose
1
10
100
Amount of Drug
X-axis
Curare is one example of a molecule called

an antagonist. Drugs that act as antagonists
Most medicines exert their effects by making
compete with natural agonists for receptors but
physical contact with receptors on the surface of
act only as decoys, freezing up the receptor and
a cell. Think of an agonist-receptor interaction
preventing agonists use of it. Researchers often
like a key tting into a lock. Inserting a key into
want to block cell responses, such as a rise in
a door lock permits the doorknob to be turned
blood pressure or an increase in heart rate. For
and allows the door to be opened. Agonists open
that reason, many drugs are antagonists, designed
cellular locks (receptors), and this is the rst step
to blunt overactive cellular responses.
12
The key to agonists tting snugly into their
major goals is to reduce these side effects by
receptors is shape. Researchers who study how
developing drugs that attach only to receptors
drugs and other chemicals exert their effects in
on the target cells.
particular organs the heart, the lungs, the
That is much easier said than done. While
kidneys, and so on are very interested in the
agonists may t nearly perfectly into a receptors
shapes of molecules. Some drugs have very broad
shape, other molecules may also brush up to
effects because they t into receptors on many
receptors and sometimes set them off. These
different kinds of cells. Some side effects, such as
types of unintended, nonspecic interactions
dry mouth or a drop in blood pressure, can result
can cause side effects. They can also affect how
from a drug encountering receptors in places other
much drug is available in the body.
than the target site. One of a pharmacologists
Steroids for Surgery

In todays culture, the word steroid conjures up
notions of drugs taken by athletes to boost strength
and physical performance. But steroid is actually
just a chemical name for any substance that has
a characteristic chemical structure consisting of
multiple rings of connected atoms. Some examples
A steroid is a molecule
with a particular chemical
structure consisting of
multiple rings (hexagons
and pentagon, below).
CH3
CH3
OH
of steroids include vitamin D, cholesterol, estrogen,

and cortisonemolecules that are critical for
keeping the body running smoothly. Various
steroids have important roles in the bodys repro
ductive system and the structure and function of
membranes. Researchers have also discovered
that steroids can be active in the brain, where they
affect the nervous system. Some steroids may
thus nd use as anesthetics, medicines that sedate
people before surgery by temporarily slowing
down brain function.
Douglas Covey of Washington University in
St. Louis, Missouri, has uncovered new roles
for several of these neurosteroids, which alter
electrical activity in the brain. Coveys research
shows that neurosteroids can either activate
or tone down receptors that communicate the
message of a neurotransmitter called gamma
aminobutyrate, or GABA. The main job of this
neurotransmitter is to dampen electrical activity
throughout the brain. Covey and other scientists
have found that steroids that activate the receptors
for GABA decrease brain activity even more,
making these steroids good candidates for anes
thetic medicines. Covey is also investigating
the potential of neuroprotective steroids in
preventing the nerve-wasting effects of certain
neurodegenerative disorders.
Bench to Bedside:
Clinical Pharmacology
Prescribing drugs is a tricky science, requiring
physicians to carefully consider many factors.
Your doctor can measure or otherwise determine
many of these factors, such as weight and diet.
But another key factor is drug interactions. You
already know that every time you go to the doctor,
he or she will ask whether you are taking any other
drugs and whether you have any drug allergies or
unusual reactions to any medicines.
Interactions between different drugs in the
body, and between drugs and foods or dietary
supplements, can have a signicant inuence,
sometimes fooling your body into thinking
you have taken more or less of a drug than you
actually have taken.
how a person is processing a drug. Usually, this

important analysis involves mathematical equa
tions, which take into account many different
variables. Some of the variables include the physi
cal and chemical properties of the drug, the total
amount of blood in a persons body, the individ
uals age and body mass, the health of the persons
liver and kidneys, and what other medicines the
person is taking. Clinical pharmacologists also
measure drug metabolites to gauge how much
drug is in a persons body. Sometimes, doctors
give patients a loading dose (a large amount)
rst, followed by smaller doses at later times. This
approach works by getting enough drug into the
body before it is metabolized (broken down) into
inactive parts, giving the drug the best chance to
do its job.
By measuring the amounts of a drug in blood

or urine, clinical pharmacologists can calculate
Natures Drugs
Feverfew for migraines, garlic for heart disease,
St. Johns wort for depression. These are just a
few of the many natural substances ingested by
millions of Americans to treat a variety of health
conditions. The use of so-called alternative medi
cines is widespread, but you may be surprised to
learn that researchers do not know in most cases
how herbs workor if they work at allinside
the human body.
Herbs are not regulated by the Food and Drug
Administration, and scientists have not performed
careful studies to evaluate their safety and effec
tiveness. Unlike many prescription (or even
over-the-counter) medicines, herbs contain many
sometimes thousandsof ingredients. While some
small studies have

conrmed the useful
ness of certain herbs,
like feverfew, other
herbal products have
proved ineffective or
harmful. For example,
recent studies suggest
that St. Johns wort is of no benet in treating
major depression. Whats more, because herbs are
complicated concoctions containing many active
components, they can interfere with the bodys
metabolism of other drugs, such as certain HIV
treatments and birth control pills.
14
Pump It Up
Bacteria have an uncanny ability to defend
the bacteria themselves. Microorganisms have
themselves against antibiotics. In trying to
ejection systems called multidrug-resistance
gure out why this is so, scientists have noted
(MDR) pumpslarge proteins that weave
that antibiotic medicines that kill bacteria in
through cell-surface membranes. Researchers
a variety of different ways can be thwarted
believe that microbes have MDR pumps
by the bacteria they are designed to destroy.
mainly for self-defense. The pumps are used
One reason, says Kim Lewis of Northeastern
to monitor incoming chemicals and to spit out
University in Boston, Massachusetts, may be
the ones that might endanger the bacteria.
LINDA S. NYE
Lewis suggests that plants, which produce
example, are often kicked out of cancer cells
many natural bacteria-killing molecules, have
by MDR pumps residing in the cells mem
gotten smart over time, developing ways to
branes. MDR pumps in membranes all over
outwit bacteria. He suspects that evolution has
the bodyin the brain, digestive tract, liver,
driven plants to produce natural chemicals that
and kidneysperform important jobs in
block bacterial MDR pumps, bypassing this
moving natural body molecules like hormones
bacterial protection system. Lewis tested his idea
into and out of cells.
Got It?
Explain the difference

between an agonist and
by rst genetically knocking out the gene for
Pharmacologist Mary Vore of the
the MDR pump from the common bacterium
University of Kentucky in Lexington has
Staphylococcus aureus (S. aureus). He and his
discovered that certain types of MDR pumps
coworkers then exposed the altered bacteria to
do not work properly during pregnancy,
How does grapefruit juice
a very weak antibiotic called berberine that had
and she suspects that estrogen and other
affect blood levels of
been chemically extracted from barberry plants.
pregnancy hormones may be partially respon
certain medicines?
Berberine is usually woefully ineffective against
sible. Vore has recently focused efforts on
S. aureus, but it proved lethal for bacteria missing
determining if the MDR pump is malformed
the MDR pump. Whats more, Lewis found
in pregnant women who have intrahepatic
that berberine also killed unaltered bacteria
cholestasis of pregnancy (ICP). A relatively
given another barberry chemical that inhibited
rare condition, ICP often strikes during the
the MDR pumps. Lewis suggests that by
third trimester and can cause signicant
co-administering inhibitors of MDR pumps
discomfort such as severe itching and nausea,
along with antibiotics, physicians may be able
while also endangering the growing fetus.
to outsmart disease-causing microorganisms.
Vores research on MDR pump function may
MDR pumps arent just for microbes.

Virtually all living things have MDR pumps,
also lead to improvements in drug therapy
an antagonist.
What does a pharmacologist plot on the vertical

and horizontal axes of a
dose-response curve?
Name one of the potential

risks associated with
taking herbal products.
for pregnant women.
including people. In the human body, MDR

pumps serve all sorts of purposes, and they can
What are the four
sometimes frustrate efforts to get drugs where
stages of a drugs life
they need to go. Chemotherapy medicines, for
in the body?
Many body molecules and drugs (yellow balls)

encounter multidrug-resistance pumps (blue)
after passing through a cell membrane.
CHAPTER 2
Body, Heal Thyself
cientists became interested in the workings

of the human body during the scientic
The Body Machine

Scientists still think about the body as a well-oiled
revolution of the 15th and 16th centuries. These
machine, or set of machines, powered by a control
early studies led to descriptions of the circulatory,
system called metabolism. The conversion of food
digestive, respiratory, nervous, and excretory
into energy integrates chemical reactions taking
systems. In time, scientists came to think of the
place simultaneously throughout the body to
body as a kind of machine that uses a series of
assure that each organ has enough nutrients and
chemical reactions to convert food into energy.
is performing its job properly. An important prin

ciple central to metabolism is that the bodys basic
unit is the cell. Like a miniature body, each cell is
surrounded by a skin, called a membrane. In turn,
each cell contains tiny organs, called organelles,
that perform specic metabolic tasks.
Discovery By Accident
The work of a scientist is often likened to locking
together the pieces of a jigsaw puzzle. Slowly and
methodically, one by one, the pieces t together to
make a pretty picture. Research is a puzzle, but the
jigsaw analogy is awed. The truth is, scientists
dont have a puzzle box to know what the nished
picture is supposed to look like. If you know the
result of an experiment ahead of time, its not really
an experiment.
Being a scientist is hard work, but most researchers
love the freedom to explore their curiosities. They test
ideas methodically, nding answers to new problems,
and every day brings a new challenge. But researchers
must keep their eyes and ears open for surprises. On
occasion, luck wins out and breakthroughs happen
by accident. The discovery of vaccines, X rays, and
penicillin each came about when a scientist was willing
to say, Hmmm, I wonder why and followed up on
an unexpected nding.
Medicines By Design I Body, Heal Thyself 17
The cell is directed by a command center, the
One important type of metabolism that occurs
nucleus, where the genes you inherited from your
constantly in our bodies is the reading and inter
parents reside. Your genesyour bodys own
preting of genes to make proteins. These proteins
personalized instruction manualare kept safe
underlie the millions of chemical reactions that
in packages called chromosomes. Each of your
run our bodies. Proteins perform structural roles,
cells has an identical set of 46 chromosomes,
keeping cells shaped properly. Proteins also work
23 inherited from your mother and 23 from
as enzymes that speed along chemical reactions
your father.
without an enzymes assistance, many reactions

would take years to happen.
Want a CYP?
Your body is a model of economy. Metabolism
your bodys way of making energy and body
parts from food and water takes place in every
cell in every organ. Complex, interlocking path
ways of cellular signals make up metabolism,
linking together all the systems that make
your body run. For this reason, researchers
have a tough time understanding the
process, because they are often faced
with studying parts one by one or a
few at a time. Nevertheless, scientists
have learned a lot by focusing on
individual metabolic pathways,
such as the one that manufactures
important regulatory
molecules called
prostaglandins
(see page 21).
Important enzymes called cytochrome
P450s (CYP, pronounced sip, 450s) process
essential molecules such as some hormones
and vitamins. The CYP 450 enzymes are
a major focus for pharmacologists because
they metabolize either break

down or activatehundreds of
prescribed medicines and natural
substances. Scientists who specialize
in pharmacogenetics (see page 8) have dis
covered that the human genetic code contains
many different spellings for CYP 450 genes, resulting
in CYP 450 proteins with widely variable levels of
activity. Some CYP 450 enzymes also metabolize
carcinogens, making these chemicals active and
more prone to causing cancer.
Toxicologist Linda Quattrochi of the University
of Colorado at Denver and Health Sciences Center
is studying the roles played by certain CYP 450
enzymes in the metabolism of carcinogens. Her
research has revealed that natural components
of certain foods, including horseradish, oranges,
mustard, and green tea, appear
to protect the body by
blocking CYP
450 enzymatic
activation of
carcinogens.
18
Red blood cells carry oxygen

throughout the body.
River of Life
Since blood is the bodys primary internal trans
portation system, most drugs travel via this route.
Medicines can nd their way to the bloodstream
in several ways, including the rich supply of blood
magical molecules that can make a clot form
vessels in the skin. You may remember, as a young
within minutes after your tumble. Blood is a rich
child, the horror of seeing blood escaping your
concoction containing oxygen-carrying red blood
body through a skinned knee. You now know that
cells and infection-ghting white blood cells.
the simplistic notion of skin literally holding
Blood cells are suspended in a watery liquid
everything inside isnt quite right. You survived
called plasma that contains clotting proteins,
the scrape just ne because blood contains
electrolytes, and many other important molecules.
Burns: More Than Skin Deep

More than simply a protective covering, skin is a
highly dynamic network of cells, nerves, and blood
vessels. Skin plays an important role in preserving
uid balance and in regulating body temperature
and sensation. Immune cells in skin help the body
prevent and ght disease. When you get burned, all
of these protections are in jeopardy. Burn-induced
skin loss can give bacteria and other microorgan
isms easy access to the nutrient-rich uids that
course through the body, while at the same time
allowing these uids to leak out rapidly. Enough
uid loss can thrust a burn or trauma patient into
shock, so doctors must replenish skin lost to severe
burns as quickly as possible.
In the case of burns covering a signicant
portion of the body, surgeons must do two things
fast: strip off the burned skin, then cover the

unprotected underlying tissue. These important
steps in the immediate care of a burn patient
took scientists decades to gure out, as they
performed carefully conducted experiments on
how the body responds to burn injury. In the early
1980s, researchers doing this work developed
the rst version of an articial skin covering called
Integra Dermal Regeneration Template, which
doctors use to drape over the area where the
burned skin has been removed. Today, Integra
Dermal Regeneration Template is used to treat
burn patients throughout the world.
Blood also ferries proteins and hormones such as
Scientists called physiologists originally came
insulin and estrogen, nutrient molecules of vari
up with the idea that all internal processes work
ous kinds, and carbon dioxide and other waste
together to keep the body in a balanced state. The
products destined to exit the body.
bloodstream links all our organs together, enabling
While the bloodstream would seem like a
them to work in a coordinated way. Two organ
quick way to get a needed medicine to a diseased
systems are particularly interesting to pharma
organ, one of the biggest problems is getting the
cologists: the nervous system (which transmits
medicine to the correct organ. In many cases,
electrical signals over wide distances) and the
drugs end up where they are not needed and cause
endocrine system (which communicates messages
side effects, as weve already noted. Whats more,
via traveling hormones). These two systems are
drugs may encounter many different obstacles
key targets for medicines.
while journeying through the bloodstream. Some

medicines get lost when they stick tightly to
certain proteins in the blood, effectively putting
the drugs out of business.
Skin consists of three layers, making up

a dynamic network of cells, nerves, and
blood vessels.
Blood Vessel
Nerve
Hair Follicle
Sweat Gland
Fat
20
No Pain, Your Gain

Like curares effects on acetylcholine, the inter
actions between another drugaspirin and
metabolism shed light on how the body works.
This little white pill has been one of the most
widely used drugs in history, and many say that
it launched the entire pharmaceutical industry.
As a prescribed drug, aspirin is 100 years old.
However, in its most primitive form, aspirin is
much older. The bark of the willow tree contains
a substance called salicin, a known antidote to
headache and fever since the time of the Greek
physician Hippocrates, around 400 B.C. The body
converts salicin to an acidic substance called salicylate.
Despite its usefulness dating back to ancient times,
early records indicate that salicylate wreaked havoc
on the stomachs of people who ingested this
natural chemical. In the late 1800s, a scientic
Salicylate
Acetylsalicylate is the aspirin

of today. Adding a chemical tag
called an acetyl group (shaded
yellow box, right) to a molecule
derived from willow bark (salicy
late, above) makes the molecule
less acidic (and easier on the
lining of the digestive tract), but
still effective at relieving pain.
Acetylsalicylate
(Aspirin)
breakthrough turned willow-derived salicylate

into a medicine friendlier to the body. Bayer
scientist Felix Hoffman discovered that adding
a chemical tag called an acetyl group (see gure,
page 20) to salicylate made the molecule less acidic
and a little gentler on the stomach, but the chemical
change did not seem to lessen the drugs ability to
relieve his fathers rheumatism. This molecule,
acetylsalicylate, is the aspirin of today.
Aspirin works by blocking the production
of messenger molecules called prostaglandins.
Because of the many important roles they play
in metabolism, prostaglandins are important
targets for drugs and are very interesting to pharma
cologists. Prostaglandins can help muscles relax and
open up blood vessels, they give you a fever when
youre infected with bacteria, and they also marshal
the immune system by stimulating the process called
inammation. Sunburn, bee stings, tendinitis,
and arthritis are just a few examples of painful
inammation caused by the bodys release of certain
types of prostaglandins in response to an injury.
Inammation leads to pain in arthritis.
22
Aspirin belongs to a diverse group of
To understand how enzymes like COX work,
medicines called NSAIDs, a nickname for
some pharmacologists use special biophysical
the tongue-twisting title nonsteroidal anti-
techniques and X rays to determine the three-
inammatory drugs. Other drugs that belong
dimensional shapes of the enzymes. These kinds
to this large class of medicines include Advil,
of experiments teach scientists about molecular
Aleve, and many other popular pain relievers
function by providing clear pictures of how all the
available without a doctors prescription. All these
folds and bends of an enzymeusually a protein
drugs share aspirins ability to knock back the
or group of interacting proteins help it do its
production of prostaglandins by blocking an
job. In drug development, one successful approach
enzyme called cyclooxygenase. Known as COX,
has been to use this information to design decoys
this enzyme is a critical driver of the bodys
to jam up the working parts of enzymes like COX.
metabolism and immune function.
Structural studies unveiling the shapes of COX
COX makes prostaglandins and other similar
enzymes led to a new class of drugs used to treat
molecules collectively known as eicosanoids from
arthritis. Researchers designed these drugs to selec
a molecule called arachidonic acid. Named for
tively home in on one particular type of COX
the Greek word eikos, meaning twenty, each
enzyme called COX-2.
eicosanoid contains 20 atoms of carbon.
By designing drugs that target only one form
Youve also heard of the popular pain reliever
of an enzyme like COX, pharmacologists may be
acetaminophen (Tylenol), which is famous for
able to create medicines that are great at stopping
reducing fever and relieving headaches. However,
inammation but have fewer side effects. For
scientists do not consider Tylenol an NSAID,
example, stomach upset is a common side effect
because it does little to halt inammation
caused by NSAIDs that block COX enzymes. This
(remember that part of NSAID stands for
side effect results from the fact that NSAIDs bind
anti-inammatory). If your joints are aching
to different types of COX enzymeseach of
from a long hike you werent exactly in shape
which has a slightly different shape. One of these
for, aspirin or Aleve may be better than Tylenol
enzymes is called COX-1. While both COX-1 and
because inammation is the thing making your
COX-2 enzymes make prostaglandins, COX-2
joints hurt.
beefs up the production of prostaglandins in sore,
inamed tissue, such as arthritic joints. In con
Our Immune Army
trast, COX-1 makes prostaglandins that protect
Scientists know a lot about the bodys organ
the digestive tract, and blocking the production
systems, but much more remains to be discovered.
of these protective prostaglandins can lead to
To design smart drugs that will seek out
stomach upset, and even bleeding and ulcers.
diseased cells and not healthy ones, researchers
Very recently, scientists have added a new
need to understand the body inside and out.
chapter to the COX story by identifying COX-3,
One system in particular still puzzles scientists:
which may be Tylenols long-sought molecular
the immune system.
target. Further research will help pharmacologists
Even though researchers have accumulated
understand more precisely how Tylenol and
vast amounts of knowledge about how our bodies
NSAIDs act in the body.
ght disease using white blood cells and thousands

of natural chemical weapons, a basic dilemma
persistshow does the body know what to ght?
The immune system constantly watches for foreign
The Anti Establishment

Common over-the-counter medicines used to treat
pain, fever, and inammation have many uses.
Here are some of the terms used to describe the
particular effects of these drugs:
ANTIPYRETICthis term means fever-reducing;

it comes from the Greek word pyresis, which
means re.
ANTI-INFLAMMATORYthis word describes a
drugs ability to reduce inammation, which can
cause soreness and swelling; it comes from the
Latin word amma, which means ame.
ANALGESICthis description refers to a medicines
ability to treat pain; it comes from the Greek word
algos, which means pain.
24
Antibodies are Y-shaped

molecules of the immune
system.
Antibodies are spectacularly specic pro

teins that seek out and mark for destruction
anything they do not recognize as
belonging to the body. Scientists have
learned how to join antibody-making
cells with cells that grow and divide continuously.
invaders and is exquisitely
This strategy creates cellular factories that work
sensitive to any intrusion perceived
around the clock to produce large quantities of
as non-self, like a
specialized molecules, called monoclonal antibodies,
transplanted organ from
that attach to and destroy single kinds of targets.
another person. This pro
Recently, researchers have also gured out how to
tection, however, can run afoul if the body
produce monoclonal antibodies in the egg whites
slips up and views its own tissue as foreign.
of chickens. This may reduce production costs of
Autoimmune disease, in which the immune system
these increasingly important drugs.
mistakenly attacks and destroys body tissue that it

believes to be foreign, can be the terrible consequence.
The powerful immune army presents signi
Doctors are already using therapeutic mono

clonal antibodies to attack tumors. A drug called
Rituxan was the rst therapeutic antibody
cant roadblocks for pharmacologists trying to
approved by the Food and Drug Administration
create new drugs. But some scientists have looked
to treat cancer. This monoclonal antibody targets
at the immune system through a different lens.
a unique tumor ngerprint on the surface of
Why not teach the body to launch an attack
immune cells, called B cells, in a blood cancer
on its own diseased cells? Many researchers are
called non-Hodgkins lymphoma. Another thera
pursuing immunotherapy as a way to treat a
peutic antibody for cancer, Herceptin, latches
wide range of health problems, especially cancer.
onto breast cancer cell receptors that signal growth
With advances in biotechnology, researchers are
to either mask the receptors from view or lure
now able to tailor-produce in the lab modied
immune cells to kill the cancer cells. Herceptins
forms of antibodiesour immune systems
actions prevent breast cancer from spreading to
front-line agents.
other organs.
Researchers are also investigating a new kind of
vaccine as therapy for diseases such as cancer.
The vaccines are not designed to prevent cancer,
but rather to treat the disease when it has already
research will point the way toward getting a
taken hold in the body. Unlike the targeted-attack
sick body to heal itself, it is likely that there
approach of antibody therapy, vaccines aim to
will always be a need for medicines to speed
recruit the entire immune system to ght off a
recovery from the many illnesses that
tumor. Scientists are conducting clinical trials of
plague humankind.
vaccines against cancer to evaluate the effectiveness

of this treatment approach.
The body machine has a tremendously com
plex collection of chemical signals that are relayed
back and forth through the blood and into and
out of cells. While scientists are hopeful that future
A Shock to the System
A body-wide syndrome caused by an infection

called sepsis is a leading cause of death in hospital
intensive care units, striking 750,000 people every
year and killing more than 215,000. Sepsis is a
serious public health problem, causing more deaths
annually than heart disease. The most severe form
of sepsis occurs when bacteria leak into the blood
stream, spilling their poisons and leading to a
dangerous condition called septic shock. Blood
pressure plunges dangerously low, the heart has
difculty pumping enough blood, and body temper

ature climbs or falls rapidly. In many cases, multiple
organs fail and the patient dies.
Despite the obvious public health importance of
nding effective ways to treat sepsis, researchers
have been frustratingly unsuccessful. Kevin Tracey
of the North Shore-Long Island Jewish Research
Institute in Manhasset, New York, has identied an
unusual suspect in the deadly crime of sepsis: the
nervous system. Tracey and his coworkers have
discovered an unexpected link between cytokines,
the chemical weapons released by the immune
system during sepsis, and a major nerve that con
trols critical body functions such as heart rate and
digestion. In animal studies, Tracey found that
electrically stimulating this nerve, called the vagus
nerve, signicantly lowered blood levels of TNF, a
cytokine that is produced when the body senses
the presence of bacteria in the blood. Further
research has led Tracey to conclude that produc
tion of the neurotransmitter acetylcholine underlies
the inammation-blocking response. Tracey is
investigating whether stimulating the vagus nerve
can be used as a component of therapy for sepsis
and as a treatment for other immune disorders.
26
A Closer Look
Seeing is believing. The clich could not

be more apt for biologists trying to
understand how a complicated
enzyme works. For decades,
researchers have isolated and
puried individual enzymes
from cells, performing experi
ments with these proteins to
nd out how they do their job
of speeding up chemical reac
tions. But to thoroughly understand a
molecules function, scientists have to take a
very, very close look at how all the atoms t
together and enable the molecular machine
to work properly.
Researchers called structural biologists are
fanatical about such detail, because it can
deliver valuable information for designing
drugseven for proteins that scientists have
One protruding end (green) of the MAO B enzyme

anchors the protein inside the cell. Body mole
cules or drugs rst come into contact with MAO B
(in the hatched blue region) and are worked on
within the enzymes active site, a cavity nestled
inside the protein (the hatched red region). To get
its job done, MAO B uses a helper molecule (yel
low), which ts right next to the active site where
the reaction takes place.
REPRINTED WITH PERMISSION FROM J. BIOL. CHEM. (2002) 277:23973-6.
HTTP://WWW.JBC.ORG
studied in the lab for a long time. For example,
Dale Edmondson of Emory University in
biologists have known for 40 years that an
Atlanta, Georgia, has recently uncovered new
enzyme called monoamine oxidase B (MAO B)
knowledge that may help researchers design
works in the brain to help recycle communica
better, more specic drugs to interfere with
tion molecules called neurotransmitters. MAO
these critical brain enzymes. Edmonson and
B and its cousin MAO A work by removing
his coworkers Andrea Mattevi and Claudia
molecular pieces from neurotransmitters, part
Binda of the University of Pavia in Italy got a
of the process of inactivating them. Scientists
crystal-clear glimpse of MAO B by determin
have developed drugs to block the actions
ing its three-dimensional structure. The
of MAO enzymes, and by doing so, help
researchers also saw how one MAO inhibitor,
preserve the levels of neurotransmitters in
Eldepryl, attaches to the MAO B enzyme,
people with such disorders as Parkinsons
and the scientists predict that their results
Name three functions
disease and depression.
will help in the design of more specic drugs
of blood.
However, MAO inhibitors have many
Got It?
Dene metabolism.
How does aspirin work?
with fewer side effects.
undesirable side effects. Tremors, increased

heart rate, and problems with sexual function
are some of the mild side effects of MAO
Give two examples of

immunotherapy.
inhibitors, but more serious problems include

seizures, large dips in blood pressure, and
difculty breathing. People taking MAO
inhibitors cannot eat foods containing the
substance tyramine, which is found in wine,
cheese, dried fruits, and many other foods.
Most of the side effects occur because drugs
that attach to MAO enzymes do not have a
perfect t for either MAO A or MAO B.
What is a technique
scientists use to study
a proteins threedimensional structure?
CHAPTER 3
Drugs From Nature, Then and Now
ong before the rst towns were built, before

written language was invented, and even
Natures Medicine Cabinet

Times have changed, but more than half of the
before plants were cultivated for food, the basic
worlds population still relies entirely on plants for
human desires to relieve pain and prolong life
medicines, and plants supply the active ingredients
fueled the search for medicines. No one knows
of most traditional medical products. Plants have
for sure what the earliest humans did to treat
also served as the starting point for countless drugs
their ailments, but they probably sought cures in
on the market today. Researchers generally agree that
the plants, animals, and minerals around them.
natural products from plants and other organisms

have been the most consistently successful source for
ideas for new drugs, since nature is a master chemist.
Drug discovery scientists often refer to these ideas as
leads, and chemicals that have desirable properties
in lab tests are called lead compounds.
Natural Cholesterol-Buster
Having high cholesterol is a signicant risk factor
for heart disease, a leading cause of death in the
industrialized world. Pharmacology research has
made major strides in helping people deal with
this problem. Scientists Michael Brown and
Joseph Goldstein, both of the University of Texas
Southwestern Medical Center at Dallas, won the
1985 Nobel Prize in physiology or medicine for
their fundamental work determining how the
body metabolizes cholesterol. This research, part
of which rst identied cholesterol receptors, led to
the development of the popular cholesterol-lowering
statin drugs such as Mevacor and Lipitor.
New research from pharmacologist David
Mangelsdorf, also at the University of Texas
Southwestern Medical Center at Dallas, is pointing
to another potential treatment for high cholesterol.
The new substance has the tongue-twisting
name guggulsterone, and it isnt really new at all.
Guggulsterone comes from the sap of the guggul
tree, a species native to India, and has been used
in Indias Ayurvedic medicine since at least 600
B.C. to treat a wide variety of ailments, including
obesity and cholesterol disorders. Mangelsdorf
and his coworker David Moore of Baylor College

of Medicine in Houston, Texas, found that guggul
sterone blocks a protein called the FXR receptor
that plays a role in cholesterol metabolism,
converting cholesterol in the blood to bile acids.
According to Mangelsdorf, since elevated levels of
bile acids can actually boost cholesterol, blocking
FXR helps to bring cholesterol counts down.
Sap from the guggul tree, a species native

to India, contains a substance that may help
ght heart disease.
Medicines By Design I Drugs From Nature, Then and Now 29
Relatively speaking, very few species of living

things on Earth have actually been seen and
only a few of these organisms to see whether they

harbor some sort of medically useful substance.
named by scientists. Many of these unidentied
Pharmaceutical chemists seek ideas for new
organisms arent necessarily lurking in uninhab
drugs not only in plants, but in any part of nature
ited places. A few years ago, for instance, scientists
where they may nd valuable clues. This includes
identied a brand-new species of millipede in a
searching for organisms from what has been called
rotting leaf pile in New York Citys Central Park,
the last unexplored frontier: the seawater that
an area visited by thousands of people every day.
blankets nearly three-quarters of Earth.
Scientists estimate that Earth is home to at least

250,000 different species of plants, and that up to
30 million species of insects crawl or y some
where around the globe. Equal numbers of species
of fungi, algae, and bacteria probably also exist.
Despite these vast numbers, chemists have tested
Cancer Therapy Sees the Light
Some forms of cancer

can be treated with
photodynamic therapy,
in which a cancer-killing
molecule is activated
by certain wavelengths
of light.
JOSEPH FRIEDBERG
A novel drug delivery system called photodynamic

therapy combines an ancient plant remedy,
modern blood transfusion techniques, and light.
Photodynamic therapy has been approved by the
Food and Drug Administration to treat several
cancers and certain types of age-related macular
degeneration, a devastating eye disease that is the
leading cause of blindness in North America and
Europe. Photodynamic therapy is also being tested
as a treatment for some skin and immune disorders.
The key ingredient in this therapy is psoralen,
a plant-derived chemical that has a peculiar prop
erty: It is inactive until exposed to light. Psoralen
is the active ingredient in a Nile-dwelling weed
called ammi. This remedy was used by ancient
Egyptians, who noticed that people became
prone to sunburn after eating the weed. Modern
researchers explained this phenomenon by discov
ering that psoralen, after being digested, goes
to the skins surface, where it is activated by the
suns ultraviolet rays. Activated psoralen attaches

tenaciously to the DNA of rapidly dividing cancer
cells and kills them. Photopheresis, a method
that exposes a psoralen-like drug to certain wave
lengths of light, is approved for the treatment
of some forms of lymphoma, a cancer of white
blood cells.
30
Ocean Medicines
More commonly known as sea squirts, tunicates
Marine animals ght daily for both food and
are a group of marine organisms that spend most
survival, and this underwater warfare is waged
of their lives attached to docks, rocks, or the
with chemicals. As with plants, researchers have
undersides of boats. To an untrained eye they look
recognized the potential use of this chemical
like nothing more than small, colorful blobs, but
weaponry to kill bacteria or raging cancer cells.
tunicates are evolutionarily more closely related
Scientists isolated the rst marine-derived cancer
to vertebrates like ourselves than to most other
drug, now known as Cytosar-U, decades ago.
invertebrate animals.
They found this chemical, a staple for treating
One tunicate living in the crystal waters of
leukemia and lymphoma, in a Caribbean sea
West Indies coral reefs and mangrove swamps
sponge. In recent years, scientists have discovered
turned out to be the source of an experimental
dozens of similar ocean-derived chemicals
cancer drug called ecteinascidin. Ken Rinehart, a
that appear to be powerful cancer cell killers.
chemist who was then at the University of Illinois
Researchers are testing these natural products
at Urbana-Champaign discovered this natural
for their therapeutic properties.
substance. PharmaMar, a pharmaceutical company
For example, scientists have unearthed several

promising drugs from sea creatures called tunicates.
based in Spain, now holds the licenses for

ecteinascidin, which it calls Yondelis, and is
Miracle Cures
CHRISTINE L. CASE
Led by the German scientist Paul

Ehrlich, a new era in pharmacology
began in the late 19th century.
Although Ehrlichs original idea
seems perfectly obvious now,
it was considered very strange
at the time. He proposed that
every disease should be treated
with a chemical specic for that
disease, and that the pharma
cologists task was to nd these
treatments by systematically
testing potential drugs.
A penicillin-secreting Penicillium
mold colony inhibits the growth
of bacteria (zig-zag smear growing
on culture dish).
The approach worked: Ehrlichs greatest triumph

was his discovery of salvarsan, the rst effective
treatment for the sexually transmitted disease
syphilis. Ehrlich discovered salvarsan after screening
605 different arsenic-containing compounds. Later,
researchers around the world had great success in
developing new drugs by following Ehrlichs meth
ods. For example, testing of sulfur-containing dyes
led to the 20th centurys rst miracle drugsthe
sulfa drugs, used to treat bacterial infections. During
the 1940s, sulfa drugs were rapidly replaced by a
new, more powerful, and safer antibacterial drug,
penicillinoriginally extracted from the soil-dwelling
fungus Penicillium.
Yondelis is an experimental
cancer drug isolated from
the marine organism
Ecteinascidia turbinata.
PHARMAMAR
conducting clinical trials on this drug. Lab tests
species of snail found in the reefs surrounding
indicate that Yondelis can kill cancer cells, and
Australia, Indonesia, and the Philippines. The
the rst set of clinical studies has shown that the
animals, called cone snails, have a unique venom
drug is safe for use in humans. Further phases of
containing dozens of nerve toxins. Some of these
clinical testingto evaluate whether Yondelis
venoms instantly shock prey, like the sting of an
effectively treats soft-tissue sarcomas (tumors of
electric eel or the poisons of scorpions and sea
the muscles, tendons, and supportive tissues)
anemones. Others cause paralysis, like the venoms
and other types of cancerare under way.
of cobras and puffer sh.
Animals that live in coral reefs almost always
Pharmacologist Baldomero Olivera of the
rely on chemistry to ward off hungry predators.
University of Utah in Salt Lake City, a native of
Because getting away quickly isnt an option in
the Philippines whose boyhood fascination with
this environment, lethal chemical brews are the
cone snails matured into a career studying them,
weaponry of choice for these slow-moving or
has discovered one cone snail poison that has
even sedentary animals. A powerful potion comes
become a potent new pain medicine. Oliveras
from one of these animals, a stunningly gorgeous
experiments have shown that the snail toxin is
32
1,000 times more powerful than morphine in

treating certain kinds of chronic pain. The snailderived drug, named Prialt by the company (Elan
Corporation, plc in Dublin, Ireland) that developed
and markets it, jams up nerve transmission in the
spinal cord and blocks certain pain signals from
K.S. MATZ
reaching the brain. Scientists predict that many

more cone snail toxins will be drug leads, since 500
A poison produced by the cone snail C. geographus

has become a powerful new pain medicine.
different species of this animal populate Earth.
Prospecting Biology?
The cancer drug Taxol

originally came from
the bark and needles
of yew trees.
Are researchers taking advantage of nature

when it comes to hunting for new medicines?
Public concern has been raised about
scientists scouring the worlds tropical rain
forests and coral reefs to look for potential
natural chemicals that may end up being
useful drugs. While it is true that rainforests
in particular are home to an extraordinarily
rich array of species of animals and plants,
many life-saving medicines derived from
natural products have been discovered in temper
ate climates not much different from our kitchens
and backyards.
Many wonder drugs have arisen from nonendangered species, such as the bark of the willow
tree, which was the original source of aspirin.
The antibiotic penicillin, from an ordinary mold, is

another example. Although scientists rst found
the chemical that became the widely prescribed
cancer drug Taxol in the bark of an endangered
species of tree called the Pacic yew, researchers
have since found a way to manufacture Taxol in
the lab, starting with an extract from pine needles
of the much more abundant European yew. In
many cases, chemists have also gured out ways
to make large quantities of rainforest- and reefderived chemicals in the lab (see main text).
Tweaking Nature
deciphered natures instructions on how to make
Searching natures treasure trove for potential
this powerful medicinal molecule. Thats impor
medicines is often only the rst step. Having tapped
tant, because researchers must harvest more than a
natural resources to hunt for new medicines, pharma
ton of Caribbean sea squirts to produce just 1 gram
ceutical scientists then work to gure out ways to
of the drug. By synthesizing drugs in a lab, scien
cultivate natural products or to make them from
tists can produce thousands more units of a drug,
scratch in the lab. Chemists play an essential role
plenty to use in patients if it proves effective
in turning marine and other natural products,
against disease.
which are often found in minute quantities, into

useful medicines.
In the case of Yondelis, chemist Elias J. Corey of
Harvard University in Boston, Massachusetts,
Scientists are also beginning to use a relatively

new procedure called combinatorial genetics
to custom-make products that dont even exist
in nature. Researchers have discovered ways to
Toxicogenetics: Poisons and Your Genes

Just as your genes help determine how you respond
to certain medicines, your genetic code can also
affect your susceptibility to illness. Why is it that
two people with a similar lifestyle and a nearly
identical environment can have such different
propensities to getting sick? Lots of factors con
tribute, including diet, but scientists believe that
an important component of disease risk is the
genetic variability of peoples reactions to chemicals
in the environment.
On hearing the word chemical, many people
think of smokestacks and pollution. Indeed, our
world is littered with toxic chemicals, some natural
and some synthetic. For example, nearly all of us
would succumb quickly to the poisonous bite of a
cobra, but it is harder to predict which of us will
develop cancer from exposure to carcinogens like
cigarette smoke.
Toxicologists are researchers who study
the effects of poisonous substances on living
organisms. One toxicologist, Serrine Lau of the
University of Texas at Austin, is trying to unravel
the genetic mystery of why people are more or
less susceptible to kidney damage after coming
into contact with some types of poisons. Lau and

her coworkers study the effects of a substance
called hydroquinone (HQ), an industrial pollutant
and a contaminant in cigarette smoke and diesel
engine exhaust. Lau is searching for genes that
play a role in triggering cancer in response to
HQ exposure. Her research and the work of other
so-called toxicogeneticists should help scientists
nd genetic signatures that can predict risk of
developing cancer in people exposed to harmful
carcinogens.
34
remove the genetic instructions for entire metabolic
effects at lower doses. The researchers found, to
pathways from certain microorganisms, alter the
their great surprise, that cyclosporine and FK506
instructions, and then put them back. This method
were chemically very different. To try to explain
can generate new and different natural products.
this puzzling result, Harvard University organic

chemist Stuart Schreiber (then at Yale University
Is It Chemistry or Genetics?
Regardless of the way researchers nd new
medicines, drug discovery often takes many unex
pected twists and turns. Scientists must train their
eyes to look for new opportunities lurking in the
outcomes of their experiments. Sometimes, side
trips in the lab can open up entirely new avenues
of discovery.
Take the case of cyclosporine, a drug
discovered three decades ago that suppresses the
immune system and thereby prevents the body from
rejecting transplanted organs. Still a best-selling
medicine, cyclosporine was a research breakthrough.
The drug made it possible for surgeons to save the
lives of many critically ill patients by transplanting
organs. But its not hard to imagine that the very
properties that make cyclosporine so powerful in
putting a lid on the immune system can cause
serious side effects, by damping immune function
too much.
Years after the discovery of cyclosporine,
researchers looking for less toxic versions of this
drug found a natural molecule called FK506 that
seemed to produce the same immune-suppressing
in New Haven, Connecticut) decided to take

on the challenge of guring out how to make
FK506 in his lab, beginning with simple chemical
building blocks.
Schreiber succeeded, and he and scientists
at Merck & Co., Inc. (Whitehouse Station, New
Jersey) used the synthetic FK506 as a tool to
unravel the molecular structure of the receptor
for FK506 found on immune cells. According
to Schreiber, information about the receptors
structure from these experiments opened his
eyes to consider an entirely new line of research.
Schreiber reasoned that by custom-making
small molecules in the lab, scientists could probe
the function of the FK506 receptor to systemati
cally study how the immune system works. Since
then, he and his group have continued to use
synthetic small molecules to explore biology.
Although Schreibers strategy is not truly genetics,
he calls the approach chemical genetics, because
the method resembles the way researchers go
about their studies to understand the functions
of genes.
In one traditional genetic approach, scientists

alter the spelling (nucleotide components) of
a gene and put the altered gene into a model
organism for example, a mouse, a plant, or
a yeast cellto see what effect the gene change
has on the biology of that organism. Chemical
genetics harnesses the power of chemistry to
custom-produce any molecule and introduce it
into cells, then look for biological changes that
result. Starting with chemicals instead of genes
gives drug development a step up. If the substance
being tested produces a desired effect, such as
stalling the growth of cancer cells, then the molecule
can be chemically manipulated in short order since
the chemist already knows how to make it.
Blending Science
These days, its hard for scientists to know what
to call themselves. As research worlds collide in
wondrous and productive ways, the lines get blurry
when it comes to describing your expertise. Craig
Crews of Yale University, for example, mixes a com
bination of molecular pharmacology, chemistry, and
genetics. In fact, because of his multiple scientic
curiosities, Crews is a faculty member in three
different Yale departments: molecular, cellular, and
developmental biology; chemistry; and pharma
cology. You might wonder how he has time to get
anything done.
Hes getting plenty doneCrews is among a
new breed of researchers delving into a growing
scientic area called chemical genetics (see main
text). Taking this approach, scientists use chemistry
to attack biological problems that traditionally have
been solved through genetic experiments such as
the genetic engineering of bacteria, yeast, and mice.
Crews goal is to explore how natural products
work in living systems and to identify new targets
for designing drugs. He has discovered how an
The herb feverfew (bachelors button) contains

a substance called parthenolide that appears to
block inammation.
inammation-ghting ingredient in the medicinal

herb feverfew may work inside cells. He found that the
ingredient, called parthenolide, appears to disable a
key process that gets inammation going. In the case
of feverfew, a handful of controlled scientic studies
in people have hinted that the herb, also known by
its plant name bachelors button, is effective in com
bating migraine headaches, but further studies are
needed to confirm these preliminary ndings.
36
TestingI, II, III
To translate pharmacology research into
Scientists conduct clinical trials in three
patient care, potential drugs ultimately have
phases (I, II, and III), each providing the
to be tested in people. This multistage process
answer to a different fundamental question
is known as clinical trials, and it has led
about a potential new drug: Is it safe? Does it
researchers to validate life-saving treatments
work? Is it better than the standard treatment?
for many diseases, such as childhood leukemia
Typically, researchers do years of basic work in
and Hodgkins disease. Clinical trials, though
the lab and in animal models before they can
costly and very time-consuming, are the only
even consider testing an experimental treat
way researchers can know for sure whether
ment in people. Importantly, scientists who
experimental treatments work in humans.
wish to test drugs in people must follow strict
rules that are designed to protect those who
the effectiveness of a drug as well as whether
volunteer to participate in clinical trials.
the drug is better than current treatments.
Special groups called Institutional Review
Phase III studies involve hundreds to thou
Boards, or IRBs, evaluate all proposed research
sands of patients, and these advanced trials
involving humans to determine the potential
typically last several years. Many phase II
risks and anticipated benets. The goal of an
and phase III studies are randomized,
IRB is to make sure that the risks are mini
meaning that one group of patients gets
Scientists are currently
mized and that they are reasonable compared
the experimental drug being tested while a
testing cone snail toxins
to the knowledge expected to be gained by
second, control group gets either a standard
for the treatment of which
performing the study. Clinical studies cannot
treatment or placebo (that is, no treatment,
health problem?
go forward without IRB approval. In addition,
often masked as a dummy pill or injection).
people in clinical studies must agree to the
Also, usually phase II and phase III studies are
terms of a trial by participating in a process
blindedthe patients and the researchers
called informed consent and signing a form,
do not know who is getting the experimental
required by law, that says they understand the
drug. Finally, once a new drug has completed
risks and benets involved in the study.
phase III testing, a pharmaceutical company
Phase I studies test a drugs safety in a few

dozen to a hundred people and are designed
to gure out what happens to a drug in the
bodyhow it is absorbed, metabolized, and
can request approval from the Food and Drug

Administration to market the drug.
Got It?
How are people protected

when they volunteer to participate in a clinical trial?
Why do plants and marine

organisms have chemicals
that could be used as
medicines?
excreted. Phase I studies usually take several

months. Phase II trials test whether or not a
drug produces a desired effect. These studies
What is a drug lead?
take longerfrom several months to a few

yearsand can involve up to several hundred
patients. A phase III study further examines
Name the rst marinederived cancer medicine.
CHAPTER 4
Molecules to Medicines
s youve read so far, the most important

goals of modern pharmacology are also
Medicine Hunting
While sometimes the discovery of potential medi
the most obvious. Pharmacologists want to design,
cines falls to researchers good luck, most often
and be able to produce in sufcient quantity,
pharmacologists, chemists, and other scientists
drugs that will act in a specic way without too
looking for new drugs plod along methodically
many side effects. They also want to deliver the
for years, taking suggestions from nature or clues
correct amount of a drug to the proper place in
from knowledge about how the body works.
the body. But turning molecules into medicines
Finding chemicals cellular targets can educate
is more easily said than done. Scientists struggle
scientists about how drugs work. Aspirins molecular
to fulll the twin challenges of drug design and
target, the enzyme cyclooxygenase, or COX
drug delivery.
(see page 22), was discovered this way in the early

1970s in Nobel Prize-winning work by pharma
cologist John Vane, then at the Royal College of
Surgeons in London, England. Another example is
colchicine, a relatively old drug that is still widely
used to treat gout, an excruciatingly painful type
of arthritis in which needle-like crystals of uric
acid clog joints, leading to swelling, heat, pain, and
WHO/TDR/STAMMERS
A Drug By Another Name
Drugs used to treat bone

ailments may be useful
for treating infectious
diseases like malaria.
As pet owners know, you can teach some

old dogs new tricks. In a similar vein, scientists
have in some cases found new uses for old
drugs. Remarkably, the potential new uses often
have little in common with a drugs product label
(its old use). For example, chemist Eric Oldeld
of the University of Illinois at Urbana-Champaign
discovered that one class of drugs called bisphos
phonates, which are currently approved to treat
osteoporosis and other bone disorders, may also
be useful for treating malaria, Chagas disease,
leishmaniasis, and AIDS-related infections like
toxoplasmosis.
Previous research by Oldeld and his coworkers
had hinted that the active ingredient in the
bisphosphonate medicines Fosamax , Actonel ,
and Aredia blocks a critical step in the metabo

lism of parasites, the microorganisms that cause
these diseases. To test whether this was true,
Oldeld gave the medicines to ve different types
of parasites, each grown along with human cells
in a plastic lab dish. The scientists found that small
amounts of the osteoporosis drugs killed the para
sites while sparing human cells. The researchers
are now testing the drugs in animal models of the
parasitic diseases and so far have obtained cures
in miceof certain types of leishmaniasis. If these
studies prove that bisphosphonate drugs work in
larger animal models, the next step will be to nd
out if the medicines can thwart these parasitic
diseases in humans.
Medicines By Design I Molecules to Medicines 39
stiffness. Lab experiments with colchicine led

scientists to this drugs molecular target, a cellscaffolding protein called tubulin. Colchicine
works by attaching itself to tubulin, causing
certain parts of a cells architecture to crumble,
and this action can interfere with a cells ability
to move around. Researchers suspect that in
the case of gout, colchicine works by halting
the migration of immune cells called granulo
cytes that are responsible for the inammation
NATIONAL AGRICULTURE LIBRARY, ARS, USDA
characteristic of gout.
Current estimates indicate that scientists
have identied roughly 500 to 600 molecular
targets where medicines may have effects in
the body. Medicine hunters can strategically
discover drugs by designing molecules to hit
these targets. That has already happened in some
Colchicine, a treatment for gout, was originally

derived from the stem and seeds of the meadow
saffron (autumn crocus).
cases. Researchers knew just what they were

looking for when they designed the successful
AIDS drugs called HIV protease inhibitors.
Previous knowledge of the three-dimensional
make blood clot and molecular signals that
structure of certain HIV proteins (the target)
instruct blood vessels to relax. What the scientists
guided researchers to develop drugs shaped to
did not know was how their candidate drug
block their action. Protease inhibitors have
would fare in clinical trials.
extended the lives of many people with AIDS.

However, sometimes even the most targeted
Sildenal (Viagras chemical name) did not

work very well as a heart medicine, but many
approaches can end up in big surprises. The New
men who participated in the clinical testing phase
York City pharmaceutical rm Pzer had a blood
of the drug noted one side effect in particular:
pressure-lowering drug in mind, when instead its
erections. Viagra works by boosting levels of a
scientists discovered Viagra, a best-selling drug
natural molecule called cyclic GMP that plays a
approved to treat erectile dysfunction. Initially,
key role in cell signaling in many body tissues.
researchers had planned to create a heart drug,
This molecule does a good job of opening blood
using knowledge they had about molecules that
vessels in the penis, leading to an erection.
40
21st-Century Science
biomedicine stems from biologys gradual transi
While strategies such as chemical genetics
tion from a gathering, descriptive enterprise to a
can quicken the pace of drug discovery, other
science that will someday be able to model and
approaches may help expand the number of
predict biology. If you think 25,000 genes is a lot
molecular targets from several hundred to several
(the number of genes in the human genome), realize
thousand. Many of these new avenues of research
that each gene can give rise to different variations
hinge on biology.
of the same protein, each with a different molecular
Relatively new brands of research that are
job. Scientists estimate that humans have hundreds
stepping onto center stage in 21st-century science
of thousands of protein variants. Clearly, theres lots
include genomics (the study of all of an organisms
of work to be done, which will undoubtedly keep
genetic material), proteomics (the study of all
researchers busy for years to come.
of an organisms proteins), and bioinformatics

(using computers to sift through large amounts
of biological data). The omics revolution in
A Chink in Cancers Armor
Doctors use the drug

Gleevec to treat a form
of leukemia, a disease in
which abnormally high
numbers of immune cells
(larger, purple circles in
photo) populate the blood.
Recently, researchers made an exciting step forward

in the treatment of cancer. Years of basic research
investigating circuits of cellular communication led
scientists to tailor-make a new kind of cancer medicine.
In May 2001, the drug Gleevec was approved to treat
a rare cancer of the blood called chronic myelogenous
leukemia (CML). The Food and Drug Administration
described Gleevecs approval as a testament to
the groundbreaking scientic research taking place
in labs throughout America.
Researchers designed this drug to halt a cellcommunication pathway that is always on in CML.
Their success was founded on years of experiments
in the basic biology of how cancer cells grow. The
discovery of Gleevec is an example of the success
of so-called molecular targeting: understanding how

diseases arise at the level of cells, then guring out
ways to treat them. Scores of drugs, some to treat
cancer but also many other health conditions, are
in the research pipeline as a result of scientists
eavesdropping on how cells communicate.
Rush Delivery
skin, nose, and lungs. Each of these methods
Finding new medicines and cost-effective ways to
bypasses the intestinal tract and can increase the
manufacture them is only half the battle. An enor
amount of drug getting to the desired site of
mous challenge for pharmacologists is guring out
action in the body. Slow, steady drug delivery
how to get drugs to the right place, a task known
directly to the bloodstreamwithout stopping
as drug delivery.
at the liver rstis the primary benet of skin
Ideally, a drug should enter the body, go
patches, which makes this form of drug delivery
directly to the diseased site while bypassing
particularly useful when a chemical must be
healthy tissue, do its job, and then disappear.
administered over a long period.
Unfortunately, this rarely happens with the typical
Hormones such as testosterone, progesterone,
methods of delivering drugs: swallowing and
and estrogen are available as skin patches. These
injection. When swallowed, many medicines made
forms of medicines enter the blood via a mesh
of protein are never absorbed into the blood
work of small arteries, veins, and capillaries in the
stream because they are quickly chewed up by
skin. Researchers also have developed skin patches
enzymes as they pass through the digestive system.
for a wide variety of other drugs. Some of these
If the drug does get to the blood from the intes
include Duragesic (a prescription-only pain
tines, it falls prey to liver enzymes. For doctors
medicine), Transderm Scop (a motion-sickness
prescribing such drugs, this rst-pass effect (see
drug), and Transderm Nitro (a blood vessel-
page 7) means that several doses of an oral drug
widening drug used to treat chest pain associated
are needed before enough makes it to the blood.
with heart disease). Despite their advantages,
Drug injections also cause problems, because they
however, skin patches have a signicant drawback.
are expensive, difcult for patients to self-administer,
Only very small drug molecules can get into the
and are unwieldy if the drug must be taken daily.
body through the skin.
Both methods of administration also result in
Inhaling drugs through the nose or mouth is
uctuating levels of the drug in the blood, which
another way to rapidly deliver drugs and bypass
is inefcient and can be dangerous.
the liver. Inhalers have been a mainstay of asthma
What to do? Pharmacologists can work around
therapy for years, and doctors prescribe nasal
the rst-pass effect by delivering medicines via the
steroid drugs for allergy and sinus problems.
42
Researchers are investigating insulin powders that
too small, and the particles will be exhaled. If
can be inhaled by people with diabetes who rely
clinical trials with inhaled insulin prove that it is
on insulin to control their blood sugar daily. This
safe and effective, then this therapy could make
still-experimental technology stems from novel
life much easier for people with diabetes.
uses of chemistry and engineering to manufacture

insulin particles of just the right size. Too large,
and the insulin particles could lodge in the lungs;
Reading a Cell MAP

Scientists try hard to listen to the noisy, garbled
discussions that take place inside and between
cells. Less than a decade ago, scientists identied
one very important cellular communication stream
called MAP (mitogen-activated protein) kinase
signaling. Today, molecular pharmacologists such
as Melanie H. Cobb of the University of Texas
Southwestern Medical Center at Dallas are studying
how MAP kinase signaling pathways malfunction
in unhealthy cells.
Protein
Phosphorylated Protein
Protein Kinase
Kinases are enzymes that

add phosphate groups
(red-yellow structures) to
proteins (green), assigning
the proteins a code. In this
reaction, an intermediate
molecule called ATP
(adenosine triphosphate)
donates a phosphate group
from itself, becoming ADP
(adenosine diphosphate).
ATP
ADP
Some of the interactions between proteins in

these pathways involve adding and taking away
tiny molecular labels called phosphate groups.
Kinases are the enzymes that add phosphate
groups to proteins, and this process is called phos
phorylation. Marking proteins in this way assigns
the proteins a code, instructing the cell to do some
thing, such as divide or grow. The body employs
many, many signaling pathways involving hun
dreds of different kinase enzymes. Some of the
important functions performed by MAP kinase
pathways include instructing immature cells how
to grow up to be specialized cell types like mus
cle cells, helping cells in the pancreas respond to
the hormone insulin, and even telling cells how to
die.
Since MAP kinase pathways are key to so many
important cell processes, researchers consider
them good targets for drugs. Clinical trials are
under way to test various molecules that, in animal
studies, can effectively lock up MAP kinase signal
ing when its not wanted, for example, in cancer
and in diseases involving an overactive immune
system, such as arthritis. Researchers predict
that if drugs to block MAP kinase signaling prove
effective in people, they will likely be used in com
bination with other medicines that treat a variety
of health conditions, since many diseases are
probably caused by simultaneous errors in multiple
signaling pathways.
by learning how to hijack molecular transporters

to shuttle drugs into cells. Gordon Amidon,
a pharmaceutical chemist at the University of
Michigan-Ann Arbor, has been studying one
particular transporter in mucosal membranes
lining the digestive tract. The transporter, called
hPEPT1, normally serves the body by ferrying
small, electrically charged particles and small
protein pieces called peptides into and out of
Proteins that snake through membranes help
transport molecules into cells.
HTTP://WWW.PHARMACOLOGY.UCLA.EDU
the intestines.
Amidon and other researchers discovered that
certain medicines, such as the antibiotic penicillin
and certain types of drugs used to treat high blood
Transportation Dilemmas
Scientists are solving the dilemma of drug delivery
pressure and heart failure, also travel into the

intestines via hPEPT1. Recent experiments
with a variety of other clever techniques. Many
revealed that the herpes drug Valtrex and the
of the techniques are geared toward sneaking
AIDS drug Retrovir also hitch a ride into intes
through the cellular gate-keeping systems

membranes. The challenge is a chemistry
problem most drugs are water-soluble, but
membranes are oily. Water and oil dont mix, and
thus many drugs cant enter the cell. To make
matters worse, size matters too. Membranes are
usually constructed to permit the entry of only
small nutrients and hormones, often through
private cellular alleyways called transporters.
Many pharmacologists are working hard to
devise ways to work not against, but with nature,
tinal cells using the hPEPT1 transporter. Amidon

wants to extend this list by synthesizing hundreds
of different molecules and testing them for their
ability to use hPEPT1 and other similar trans
porters. Recent advances in molecular biology,
genomics, and bioinformatics have sped the search
for molecules that Amidon and other researchers
can test.
44
Scientists are also trying to slip molecules
Act Like a Membrane
through membranes by cloaking them in disguise.
Researchers know that high concentrations of
Steven Regen of Lehigh University in Bethlehem,
chemotherapy drugs will kill every single cancer
Pennsylvania, has manufactured miniature
cell growing in a lab dish, but getting enough of
chemical umbrellas that close around and shield
these powerful drugs to a tumor in the body with
a molecule when it encounters a fatty membrane
out killing too many healthy cells along the way
and then spread open in the watery environment
has been exceedingly difcult. These powerful
inside a cell. So far, Regen has only used test mole
drugs can do more harm than good by severely
cules, not actual drugs, but he has succeeded in
sickening a patient during treatment.
getting molecules that resemble small segments
Some researchers are using membrane-like
of DNA across membranes. The ability to do this
particles called liposomes to package and deliver
in humans could be a crucial step in successfully
drugs to tumors. Liposomes are oily, microscopic
delivering therapeutic molecules to cells via
capsules that can be lled with biological cargo,
gene therapy.
such as a drug. They are very, very small only
Anesthesia Dissected
Scientists who study anesthetic medicines
have a daunting task for the most part,
they are shooting in the dark when
it comes to identifying the molecular
targets of these drugs. Researchers do
know that anesthetics share one common
ingredient: Nearly all of them somehow
target membranes, the oily wrappings
surrounding cells. However, despite the
fact that anesthesia is a routine part of
surgery, exactly how anesthetic medicines
work in the body has remained a mystery for more
than 150 years. Its an important problem, since
anesthetics have multiple effects on key body func
tions, including critical processes such as breathing.
Scientists dene anesthesia as a state in which
no movement occurs in response to what should
be painful. The problem is, even though a patient
loses a pain response, the anesthesiologist cant
tell what is happening inside the persons organs
and cells. Further complicating the issue, scientists
know that many different types of drugswith
little physical resemblance to each other can all

produce anesthesia. This makes it difcult to track
down causes and effects.
Anesthesiologist Robert Veselis of the Memorial
Sloan-Kettering Institute for Cancer Research in New
York City claried how certain types of these mys
terious medicines work. Veselis and his coworkers
measured electrical activity in the brains of healthy
volunteers receiving anesthetics while they listened
to different sounds. To determine how sedated the
people were, the researchers measured reaction
time to the sounds the people heard. To measure
memory effects, they quizzed the volunteers at the
end of the study about word lists they had heard
before and during anesthesia. Veselis experiments
show that the anesthetics they studied affect sepa
rate brain areas to produce the two different effects
of sedation and memory loss. The ndings may help
doctors give anesthetic medicines more effectively
and safely and prevent reactions with other drugs
a patient may be taking.
one one-thousandth the width of a single human

hair. Researchers have known about liposomes
for many years, but getting them to the right place
in the body hasnt been easy. Once in the blood
LAWRENCE MAYER, LUDGER ICKENSTEIN, KATRINA EDWARDS
stream, these foreign particles are immediately

shipped to the liver and spleen, where they
are destroyed.
Materials engineer David Needham of Duke
University in Durham, North Carolina, is investi
gating the physics and chemistry of liposomes to
better understand how the liposomes and their
cancer-ghting cargo can travel through the body.
Needham worked for 10 years to create a special
David Needham designed liposomes resembling tiny molecular soccer

balls made from two different oils that wrap around a drug.
kind of liposome that melts at just a few degrees

above body temperature. The end result is a tiny
dogs revealed that, when heated, the drug-laden
molecular soccer ball made from two different
capsules ooded tumors with a chemotherapy
oils that wrap around a drug. At room tempera
drug and killed the cancer cells inside. Researchers
ture, the liposomes are solid and they stay solid at
hope to soon begin the rst stage of human studies
body temperature, so they can be injected into the
testing the heat-triggered liposome treatment in
bloodstream. The liposomes are designed to spill
patients with prostate and breast cancer. The results
their drug cargo into a tumor when heat is applied
of these and later clinical trials will determine
to the cancerous tissue. Heat is known to perturb
whether liposome therapy can be a useful weapon
tumors, making the blood vessels surrounding
for treating breast and prostate cancer and other
cancer cells extra-leaky. As the liposomes approach
hard-to-treat solid tumors.
the warmed tumor tissue, the stitches of the

miniature soccer balls begin to dissolve, rapidly
leaking the liposomes contents.
Needham and Duke oncologist Mark Dewhirst
teamed up to do animal studies with the heatactivated liposomes. Experiments in mice and
46
The G Switch
(b)
(a)
(c)
Hormone
Plasma Membrane
Receptor
Active Cell Enzyme
Inactive Cell Enzyme

Inactive G Protein
Active G Protein
Cell Response
G proteins act like relay batons to pass

messages from circulating hormones
into cells.
(a) A hormone (red) encounters a receptor
(blue) in the membrane of a cell.
(b) A G protein (green) becomes activated
and makes contact with the receptor
to which the hormone is attached.
(c) The G protein passes the hormones
message to the cell by switching on
a cell enzyme (purple) that triggers
a response.
Imagine yourself sitting on a cell, looking

outward to the bloodstream rushing by.
Suddenly, a huge glob of something hurls
toward you, slowing down just as it settles
into a perfect dock on the surface of your
cell perch. You dont realize it, but your own
body sent this substancea hormone called
epinephrineto protect you, telling you to
get out of the way of a car that just about side
swiped yours while drifting out of its lane.
Your body reacts, whipping up the familiar,
spine-tingling, ght-or-ight response that
gears you to respond quickly to potentially
threatening situations such as this one.
How does it all happen so fast?
Getting into a cell is a challenge, a strictly
guarded process kept in control by a protective
gate called the plasma membrane. Figuring
out how molecular triggers like epinephrine
communicate important messages to the
inner parts of cells earned two scientists the
Nobel Prize in physiology or medicine in
1994. Getting a cellular message across the
membrane is called signal transduction, and it
the world have focused on these signaling
occurs in three steps. First, a message (such as
molecules. Research on G proteins and on all
epinephrine) encounters the outside of a cell
aspects of cell signaling has prospered, and as
and makes contact with a molecule on the
a result scientists now have an avalanche of
surface called a receptor. Next, a connecting
data. In the fall of 2000, Gilman embarked on
transducer, or switch molecule, passes the
a groundbreaking effort to begin to untangle
message inward, sort of like a relay baton.
and reconstruct some of this information to
Finally, in the third step, the signal gets ampli
guide the way toward creating a virtual cell.
ed, prompting the cell to do something:
Gilman leads the Alliance for Cellular
move, produce new proteins, even send out
Signaling, a large, interactive research network.
more signals.
The group has a big dream: to understand
One of the Nobel Prize winners, pharma
Got It?
What is a liposome?
Name three drug

delivery methods.
everything there is to know about signaling
cologist Alfred G. Gilman of the University of
inside cells. According to Gilman, Alliance
Describe how
Texas Southwestern Medical Center at Dallas,
researchers focus lots of attention on G
G proteins work.
uncovered the identity of the switch molecule,
proteins and also on other signaling systems
called a G protein. Gilman named the switch,
in selected cell types. Ultimately, the scientists
which is actually a huge family of switch mol
hope to test drugs and learn about disease
ecules, not after himself but after the type of
through computer modeling experiments
cellular fuel it uses: an energy currency called
with the virtual cell system.
GTP. As with any switch, G proteins must be

turned on only when needed, then shut off.
Some illnesses, including fatal diseases like
cholera, occur when a G protein is errantly
left on. In the case of cholera, the poisonous
weaponry of the cholera bacterium freezes
in place one particular type of G protein that
controls water balance. The effect is constant
uid leakage, causing life-threatening diarrhea.
In the few decades since Gilman and the
other Nobel Prize winner, the late National
Institutes of Health scientist Martin Rodbell,
made their fundamental discovery about
G protein switches, pharmacologists all over
What do kinases do?
Discuss the omics

revolution in biomedical
research.
48
Medicines for the Future
he advances in drug development and

delivery described in this booklet reect
scientists growing knowledge about human

biology. This knowledge has allowed them to
develop medicines targeted to specic molecules
or cells. In the future, doctors may be able to treat
or prevent diseases with drugs that actually repair
cells or protect them from attack. No one knows
which of the techniques now being developed will
yield valuable future medicines, but it is clear that
thanks to pharmacology research, tomorrows
doctors will have an unprecedented array of
weapons to ght disease.
Medicines By Design I Medicines for the Future 49
Careers in Pharmacology
Wanna be a pharmacologist? If you choose pharma
cology as a career, here are some of the places you
might nd yourself working:
College or University. Most basic biomedical
research across the country is done by scientists
at colleges and universities. Academic pharma
cologists perform research to determine how
medicines interact with living systems. They also
teach pharmacology to graduate, medical, pharmacy,
veterinary, dental, or undergraduate students.
Pharmaceutical Company. Pharmacologists
who work in industry participate in drug develop
ment as part of a team of scientists. A key aspect
of pharmaceutical industry research is making
sure new medicines are effective and safe for use
in people.
Hospital or Medical Center. Most clinical pharma
cologists are physicians who have specialized
training in the use of drugs and combinations of
drugs to treat various health conditions. These

scientists often work with patients and spend a
lot of time trying to understand issues relating
to drug dosage, including side effects and
drug interactions.
Government Agency. Pharmacologists and
toxicologists play key roles in formulating drug
laws and chemical regulations. Federal agencies
such as the National Institutes of Health and the
Food and Drug Administration hire many pharma
cologists for their expertise in how drugs work.
These scientists help develop policies about the
safe use of medicines.
You can learn more about careers in pharmacology
by contacting professional organizations such
as the American Society for Pharmacology and
Experimental Therapeutics (http://www.aspet.org/)
or the American Society for Clinical Pharmacology
and Therapeutics (http://www.ascpt.org/).
50
Glossary
ADME | Abbreviation for the four steps in a
Bioavailability | The ability of a drug or other
medicines journey through the body: absorption,
chemical to be taken up by the body and made
distribution, metabolism, and excretion.
available in the tissue where it is needed.
Agonist | A molecule that triggers a cellular
Bioinformatics | A eld of research that relies
response by interacting with a receptor.
on computers to store and analyze large amounts
Analgesic | A medicines ability to relieve pain,
of biological data.
or a drug that alleviates pain; the term comes from
Biotechnology | The industrial use of living
the Greek word algos, which means pain.
organisms or biological methods derived through
Antagonist | A molecule that prevents the
basic research.
action of other molecules, often by competing
Biotransformation | The conversion of a
for a cellular receptor; opposite of agonist.
substance from one form to another by the
Antibiotic | A substance that can kill or inhibit

the growth of certain microorganisms.
Antibody | A protein of the immune system,
produced in response to an antigen (a foreign,
often disease-causing, substance).
Anti-inammatory | A drugs ability to
reduce inammation, which can cause soreness
and swelling.
Antipyretic | Fever-reducing; the term comes
from the Greek word pyresis, which means re.
Arachidonic acid | A molecule that synthesizes
regulatory molecules such as prostaglandins; it is
actions of organisms or enzymes.

Blood-brain barrier | A blockade consisting
of cells and small blood vessels that limits the
movement of substances from the bloodstream
into the brain.
Carcinogen | Any substance that, when exposed
to living tissue, may cause cancer.
Cell | The basic subunit of any living organism;
the simplest unit that can exist as an independent
living system.
Central nervous system | The brain and
spinal cord.
found in fatty animal tissue and foods such as egg
Chemical bond | Physical force holding atoms
yolk and liver.
together to form a molecule.
Bacterium | One-celled organism without
Chemical genetics | A research approach
a nucleus that reproduces by cell division; can
resembling genetics in which scientists custom-
infect humans, plants, or animals.
produce synthetic, protein-binding small

molecules to explore biology.
Medicines By Design I Glossary 51
Cholesterol | A lipid unique to animal cells that
Dose-response curve | A graph drawn to
is used in the construction of cell membranes and
show the relationship between the dose of a drug
as a building block for some hormones.
or other chemical and the effect it produces.
Chromosome | A structure in the cell nucleus
Enzyme | A molecule (usually a protein) that
that contains hereditary material (genes); humans
speeds up, or catalyzes, a chemical reaction with
have 23 pairs of chromosomes in each body cell,
out being permanently altered or consumed.
one of each pair from the mother and the other

from the father.
Essential fatty acid | A long, fat-containing

molecule involved in human body processes that
Clinical trial | A scientic study to determine
is synthesized by plants but not by the human
the effects of potential medicines in people;
body and is therefore a dietary requirement.
usually conducted in three phases (I, II, III), to

determine whether the drug is safe, effective,
First-pass effect | The breakdown of orally

administered drugs in the liver and intestines.
and better than current therapies, respectively.

G protein | One of a group of switch proteins
Combinatorial genetics | A research process
in which scientists remove the genetic instructions
involved in a signaling system that passes incoming

messages across cell membranes and within cells.
for entire metabolic pathways from certain

microorganisms, alter the instructions, and
then put them back.
Gene | A unit of heredity; a segment of a DNA

molecule containing the code for making a protein
or, sometimes, an RNA molecule.
Cyclooxygenase | An enzyme, also known as

COX, that makes prostaglandins from a molecule
called arachidonic acid; the molecular target of
nonsteroidal anti-inammatory drugs.
Cytochrome P450 | A family of enzymes
found in animals, plants, and bacteria that have
an important role in drug metabolism.
DNA (deoxyribonucleic acid) | A doublestranded, helical molecule that encodes genetic
information.
Genetics | The scientic study of genes and

heredity, of how particular qualities or traits are
transmitted from parents to offspring.
Genomics | The study of all of an organisms
genetic material.
Hormone | A messenger molecule that helps
coordinate the actions of various tissues; made
in one part of the body and transported, via the
bloodstream, to tissues and organs elsewhere in
the body.
Dose | The amount of medicine to be taken

at one time.
52
Immunotherapy | A medical treatment to
Model organism | A bacterium, animal, or
stimulate a patients immune system to attack
plant used by scientists to study basic research
and destroy disease-causing cells.
questions; common model organisms include
Inammation | The bodys characteristic
yeast, ies, worms, frogs, and sh.
reaction to infection or injury, resulting in
Monoclonal antibody | An antibody that rec
redness, swelling, heat, and pain.
ognizes only one type of antigen; sometimes used
Informed consent | The agreement of a person
as immunotherapy to treat diseases such as cancer.
(or his or her legally authorized representative) to
NSAID (nonsteroidal anti-inammatory
serve as a research subject, with full knowledge of
drug) | Any of a class of drugs that reduces pain,
all anticipated risks and benets of the experiment.
fever, or inammation by interfering with the
Kinase | An enzyme that adds phosphate groups

to proteins.
Lipid | A fatty, waxy, or oily molecule that
will not dissolve in water; it contains hydrogen,
carbon, and oxygen.
Liposome | Oily, microscopic capsules designed
to package and deliver biological cargo, such as
drugs, to cells in the body.
Membrane | A thin covering surrounding a cell
and separating it from the environment; consists
of a double layer of molecules called phospholipids
and has proteins embedded in it.
Metabolism | All enzyme-catalyzed reactions
in a living organism that builds and breaks down
organic molecules, producing or consuming
synthesis of prostaglandins.
Neurotransmitter | A chemical messenger that
allows neurons (nerve cells) to communicate with
each other and with other cells.
Nucleus | The membrane-bound structure
within a cell that contains most of the cells
genetic material.
Organelle | A specialized, membrane-bound
structure that has a dened cellular function;
for example, the nucleus.
Peptide | A small protein fragment.
Pharmacodynamics | The study of how drugs
act at target sites of action in the body.
Pharmacogenetics | The study of how peoples
genes affect their response to medicines.
energy in the process.

Pharmacokinetics | The study of how the
Metabolite | A chemical intermediate in
metabolic reactions; a product of metabolism.
body absorbs, distributes, breaks down, and

eliminates drugs.
Medicines By Design I Glossary 53
Pharmacologist | A scientist focusing
Recombinant DNA technology | Modern
on pharmacology.
techniques in molecular biology to manipulate an
Pharmacology | The study of how drugs

interact with living systems.
Pharmacy | An area in the health sciences
that deals with the preparation, dispensing,
and appropriate use of medicines.
organisms genes by introducing, eliminating, or

changing genes.
RNA (ribonucleic acid) | A molecule that
serves as an intermediate step in the synthesis of
proteins from instructions coded in DNA; some
RNA molecules also perform regulatory functions
Physiology | The study of how living
in cells and viruses.
organisms function.
Sepsis | A clinical condition in which infectious
Prostaglandins | Any of a class of hormonelike, fat-soluble, regulatory molecules made from
fatty acids such as arachidonic acid; prostaglandins
agents (bacteria, fungi) or products of infection

(bacterial toxins) enter the blood and profoundly
affect body systems.
participate in diverse body functions, and their

production is blocked by NSAIDs.
Side effect | The effect of a drug, other than

the desired effect, sometimes in an organ other
Protein | A large molecule composed of one or
than the target organ.
more chains of amino acids (the building blocks

of proteins) in a specic order and a folded
shape determined by the sequence of nucleotides
in the gene encoding the protein; essential for all
life processes.
Proteomics | The systematic, large-scale study
of all proteins in an organism.
Receptor | A specialized molecule that receives
information from the environment and conveys
Signal transduction | The process by which

a hormone or growth factor outside the cell
transmits a message into the cell.
Site of action | The place in the body where a
drug exerts its effects.
Steroid | A type of molecule that has a multiple
ring structure, with the rings sharing molecules
of carbon.
it to other parts of the cell; the information is
Structural biology | A eld of study dedicated
transmitted by a specic chemical that must t
to determining the three-dimensional structures
the receptor, like a key in a lock.
of biological molecules to better understand the

function of these molecules.
54
Therapeutic drug | A drug used to treat a

disease or condition; contrast with drug of abuse.
Toxicology | The study of how poisonous
substances interact with living organisms.
Virus | An infectious agent composed of a
protein coat around a DNA or RNA core; to
reproduce, viruses depend on living cells.
X-ray crystallography | A technique used
to determine the detailed, three-dimensional
structure of molecules based on the scattering
of X rays through a crystal of the molecule.
What Is NIGMS?
Discrimination Prohibited
Accessibility
The National Institute of General Medical Sciences
Under provisions of applicable public laws enacted
This publication can be made available in
(NIGMS) supports basic biomedical research on
by Congress since 1964, no person in the United
formats that are more accessible to people
genes, proteins, and cells. It also funds studies on
States shall, on the grounds of race, color, national
with disabilities. To request this material in a
fundamental processes such as how cells commu-
origin, handicap, or age, be excluded from partici
different format, contact the NIGMS Ofce
nicate, how our bodies use energy, and how we
pation in, be denied the benets of, or be subjected
of Communications and Public Liaison at
respond to medicines. The results of this research
to discrimination under any program or activity
301-496-7301, TDD 301-402-6327; send e-mail
increase our understanding of life and lay the
(or, on the basis of sex, with respect to any educa
to info@nigms.nih.gov; or write to the ofce at
foundation for advances in the diagnosis, treatment,
tion program or activity) receiving Federal nancial
the following address: 45 Center Drive MSC
and prevention of disease. The Institutes research
assistance. In addition, Executive Order 11141
6200, Bethesda, MD 20892-6200. If you have
training programs produce the next generation of
prohibits discrimination on the basis of age by
questions about this publication, you can use
biomedical scientists, and NIGMS has programs to
contractors and subcontractors in the performance
the same contact information to reach the writer,
encourage minorities underrepresented in biomedical
of Federal contracts, and Executive Order 11246
Alison Davis, Ph.D.
and behavioral science to pursue research careers.
states that no federally funded contractor may
NIGMS supported the research of most of the
discriminate against any employee or applicant
scientists mentioned in this booklet.
for employment because of race, color, religion,

sex, or national origin. Therefore, the programs of
Disclaimer
Trade names have been used throughout this
booklet to illustrate concepts about medicines
that are familiar to readers. The mention of
specic products is not an endorsement of their
use or effectiveness.
the National Institute of General Medical Sciences

must be operated in compliance with these laws
and Executive Orders.
Additional Copies
and Web Links
To order additional copies of Medicines By Design
DEPT OF HEALTH AND HUMAN SERVICES

OFFICIAL BUSINESS
PENALTY FOR PRIVATE USE $300
or other free publications available from

NIGMS, go to http://publications.nigms.nih.gov/
order/classroom.htm or use the contact
information above.
Medicines By Design is available online at

http://publications.nigms.nih.gov/medbydesign.

OFFICE OF COMMUNICATIONS AND PUBLIC LIAISON
AT T E N T I O N R E A D E R S
We would like your comments on Medicines By Design.

Please give us your opinion by lling out this postage-paid response card.
1.
Extent to which the publication held your interest
2.
Understandability
3.
Amount and type of information presented
4.
Join our Findings mailing list. For sample issues,

see http://publications.nigms.nih.gov/ndings.
q I would like to receive Findings, a magazine that
profiles two NIGMS-supported scientists, features

brief descriptions of recent clinically relevant
research, and includes a crossword puzzle based
on words used in the articles.
q I would like to receive a free CD-ROM containing
NIGMS science education booklets on topics such as

cell biology, chemistry, genetics, pharmacology, and
structural biology. These booklets are geared toward
a high school and early college audience. Print
copies of the publications can be ordered from
http://www.nigms.nih.gov/publications/classroom
Usefulness and value of such a publication
Medicines By Design
Please comment on whether Medicines By Design helped you

learn more about:
Name
1.
Pharmacology research
2.
What its like to be a scientist
Address
State
3.
The excitement of biomedical research today
Zip Code

E-mail (optional)
Phone (optional)
Other Comments:
U.S. DEPARTMENT OF
City

Reprinted July 2006
U.S. DEPARTMENT OF


Medicines by Design: U.S. Department of Health and Human Services

Uploaded by

Copyright:

Available Formats

Medicines by Design: U.S. Department of Health and Human Services

Uploaded by

Document Information

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Medicines by Design: U.S. Department of Health and Human Services

Uploaded by

Copyright:

Available Formats

AT T E N T I O N R E A D E R S

We would like your comments on Medicines By Design.

Extent to which the publication held your interest

Amount and type of information presented

Join our Findings mailing list. For sample issues,

q I would like to receive Findings, a magazine that

profiles two NIGMS-supported scientists, features

q I would like to receive a free CD-ROM containing

NIGMS science education booklets on topics such as

Usefulness and value of such a publication

Please comment on whether Medicines By Design helped you

What its like to be a scientist

The excitement of biomedical research today

National Institutes of Health

NIH Publication No. 06-474

National Institutes of Health

The National Institute of General Medical Sciences

Under provisions of applicable public laws enacted

This publication can be made available in

(NIGMS) supports basic biomedical research on

by Congress since 1964, no person in the United

formats that are more accessible to people

genes, proteins, and cells. It also funds studies on

States shall, on the grounds of race, color, national

with disabilities. To request this material in a

fundamental processes such as how cells commu

origin, handicap, or age, be excluded from partici-

different format, contact the NIGMS Ofce

nicate, how our bodies use energy, and how we

pation in, be denied the benets of, or be subjected

of Communications and Public Liaison at

respond to medicines. The results of this research

to discrimination under any program or activity

301-496-7301, TDD 301-402-6327; send e-mail

increase our understanding of life and lay the

(or, on the basis of sex, with respect to any educa-

to info@nigms.nih.gov; or write to the ofce at

foundation for advances in the diagnosis, treatment,

tion program or activity) receiving Federal nancial

the following address: 45 Center Drive MSC

and prevention of disease. The Institutes research

assistance. In addition, Executive Order 11141

6200, Bethesda, MD 20892-6200. If you have

training programs produce the next generation of

prohibits discrimination on the basis of age by

questions about this publication, you can use

biomedical scientists, and NIGMS has programs to

contractors and subcontractors in the performance

the same contact information to reach the writer,

encourage minorities underrepresented in biomedical

of Federal contracts, and Executive Order 11246

Alison Davis, Ph.D.

and behavioral science to pursue research careers.

states that no federally funded contractor may

NIGMS supported the research of most of the

discriminate against any employee or applicant

scientists mentioned in this booklet.

for employment because of race, color, religion,

the National Institute of General Medical Sciences