Glovebox Operating Instructions Mbraun

Chapter 1 - General Information
Chapter 2 - Liability, Warranty and Safety

Chapter 3 - Principles of Operation
Chapter 4 - Touch Panel Operation
Chapter 5 - System Screens
Chapter 6 - Installation Requirements
Chapter 7 - Installation
Chapter 8 - Commissioning the System
Chapter 9 - System Operation
Chapter 10 - Maintenance & Troubleshooting
Edition Information:
Edition January 2007
Revision Information:
M.Braun, Inc.
14 Marin Way Stratham, NH 03885
Phone 603-773-9333 Fax 603-773-0008
Email: info@mbraun.com
www.mbraunusa.com
Revision 002
Title
Operating Instructions Supplement A
Technical Consultation:
K. Prince, Programming
T. Daly, Service
Technical Assistance:
K. Prince, Programming
T. Daly, Service
Layout:
B. Adams, Quality Manager, M.Braun, Inc.
Publication edited by means of:
Adobe Acrobat, Adobe Photoshop, Microsoft Word
Important Notes:
This technical documentation is not liable to any

obligations on the part of the manufacturer. The
manufacturer M.Braun, Inc. reserves the right for
technical and optical modifications as well as functional
modifications on the system or systems components
described therein.
Any duplication of this documentation, even in the
forms of excerpts, is only permitted after having
obtained the manufacturers information and
concession.
Chapter 1
1.1 Liability....1-1
General Information 1.2 Contact Information....1-2
Operating Instructions Labmaster 130 and MB20G Systems

Instruction Manual
Chapter 1: General Information
CHAPTER 1: GENERAL INFORMATION

1.1 Entries Referring to the System
This documentation is part of the system:
Designation / Type:
Series Number(s):
Persons In Charge of System:
Space left for notes on system settings, instructions for maintenance, etc.
MBraun, Inc. Edition 1/2007
Page 1-1

Instruction Manual
Chapter 1: General Information
1.2 Contact Information

M.Braun, Inc.
14 Marin Way
Stratham, NH 03885
Phone:
Fax:
Email:
Internet:
603-773-9333
603-773-0008
service@mbraunusa.com
www.mbraunusa.com
Page 1-2
Chapter 2
2.1 General Information....2-1
Liability, Warranty 2.2 Liability....2-1

and Safety
2.3 Warranty....2-2
2.4 Operation Guidelines....2-2
2.5 Safety - General Hazards....2-3
2.6 Safety - Mechanical....2-3
2.7 Safety - Electrical....2-4
2.8 Safety - Handling of Electronic Components....2-5
2.9 Safety - Chemicals and Gases....2-5
2.10 Safety - Symbols Used on the System....2-6
2.11 Safety - Emergencies....2-6
2.12 Additional Information....2-7

Instruction Manual
Chapter 2: Liability, Warranty & Safety
CHAPTER 2: LIABILITY, WARRANTY & SAFETY

2.1 General Information
Prior to installation, initialization and operation of the system, this manual should be read in its
entirety and positioned in a suitable area near the system to allow for easy reference. Any
persons charged with the transport, storing, installation, commissioning, operating, maintenance
and/or service of this system must be familiar with the entire contents of this manual.
To ensure safe operation of the system and to maintain a safe working environment, the
information contained within this chapter must be adhered to by all users of the system. Advice
contained in this chapter is intended to supplement, not supersede, the safety advice given in
other chapters of this manual and the general safety regulations and guidelines prevailing in the
users workplace.
In addition to the guidelines and information contained within this manual all internal and local
health, safety and environmental guidelines should be followed.
Safety instructions and pertinent information are marked in the following manner:
Indicates an imminently hazardous situation which, if not avoided,
will result in death, serious injury or serious damage to the system,
other equipment or surrounding environment.
Indicates a potentially hazardous situation which, if not avoided,
could result in death, serious injury or serious damage to the
system, other equipment or surrounding environment.
Indicates a potentially hazardous situation which, if not avoided,
may result in minor or moderate injury or damage to the system,
other equipment or surrounding environment.
Indicates additional advice or recommendations for using the
system or completing a task.
Please consider all instructions, in particular safety instructions, in order to achieve safe
operation of the system.
2.2 Liability
The manufacturer will not take any liability of object, personal or secondary damage caused by
improper use or ignoring of safety instructions as well as caused by the owners manual due to
missing updates after the system or its software have been modified, nor will the manufacturer
take any liability of damages due to loss of data. In addition, the terms of business that are part
of the order/contract will apply.
Our products are continuously modified an improved due to innovation, legal requirements and
standards. Consequently, the information give in this documentation may not accurately reflect
every detail of the system actually delivered. Please contact the manufacturer in cases of
uncertainty.
Page 2-1

Instruction Manual
2.3 Warranty
We guarantee the equipment as stated in the order/contract.
This warranty will expire in case of:
Interference into or modification of the system without prior consent of the manufacturer;
Improper use of the system;
Insufficient maintenance of the system;
Inappropriate operation of the system;
Negligence of correct supply requirements;
Application of third-part components to the system without prior consent of the
manufacturer;
Alteration of program or configuration write-ups without manufacturers consent.
This applies to a single unit and multi-unit system types.
2.4 Operation Guidelines

MBRAUN glovebox systems are operated using inert gas enabling the user to handle
substances which are sensitive to oxygen and/or moisture. It is the responsibility of the user to
follow all local health, safety and environmental guidelines with regards to the handling and
disposing of substances which may be injurious to health. This also applies to the disposal of
vacuum pump oil and all components and filtering devices which come in contact with the gas
flow.
There is a risk of suffocation when working with high inert gas
concentrations.
On request, MBRAUN can recommend a personal measuring instrument that alerts the
operator to a reduction of oxygen content in the ambient air.
The following general safety guidelines must be considered when working with inert gas
concentrations:
The selected location should have a room volume that is significantly larger than the
glovebox interior volume.
The system should be located in a well ventilated area. This is especially important
during a purging procedure or when opening an active system (i.e. antechambers, etc.).
All exhaust fumes should be vented through an adequate disposal/ventilation system.
Contact MBRAUN prior to the acquisition of the system if it is not
possible to adhere to all the above recommendations. This way the
system can be equipped with additional safety devices.
Prior to performing maintenance or service inside an active system, remove one glove to
allow a slow equalization of the glovebox interior atmosphere with the ambient room air.
To better avoid the risk of suffocation, it is necessary for the
glovebox atmosphere to be completely replaced with ambient room
air prior to servicing the interior of an active glovebox.
Standard MBRAUN glovebox systems are not designed for the use
of strongly poisonous or radioactive substances. Use of these types
of materials must be coordinated with MBRAUN prior to the
acquisition of a system.
Page 2-2

Instruction Manual
2.5 Safety General Hazards

This system has been designed and manufactured considering all relevant safety regulations.
Improper use or operation by persons not qualified accordingly may result in danger to the:
Life and health of the operator;
System itself;
Surroundings of the user;
Performance and efficiency of the system.
General hazards of the system may arise in the following ways:
Mechanical hazard caused by squeezing, shearing and cutting, catching and winding,
stretching or by freely moving parts;
Thrust caused by kinetic energy of moving mass;
Sharp corners and edges;
Electrical hazard caused by touching live parts (directly or indirectly);
Thermal hazard causing burns;
Chemical hazard causing poisoning, corrosion and explosion;
Toxic hazard due to inhalation of vapours and gases;
Gases under pressure;
Liquids under pressure;
Combination of hazards caused by:
faulty installation
incorrect loading
breakdown of power or media supply
breakdown and/or incorrect arrangement of preventive measures
combination of escaping media
Hazards caused by:
human misconduct
noise
allergies, excitations of mucous membrane, unknown effects caused by media
ejection of parts
disturbance / malfunction of control system
leaking of hoses or pipes
combination of atmospheres or vapors
fire hazard
natural hazards e.g. lightning, flooding, environmental catastrophes etc.
2.6 Safety Mechanical

Freely moving parts may cause squeezing, shearing and cutting,
catching and winding, stretching of extremities. Extreme caution
should be taken to avoid touching any moving parts of the system
during operation.
When handling materials with mechanical, pneumatic or vacuum systems it is possible that
materials may be ejected. Extreme caution should be taken to avoid any possible contact with
the ejected materials without proper protection.
Only genuine parts supplied by MBRAUN should be used in the operation of the system. These
parts are constructed in conformance with applicable safety regulations. No liability will be taken
by MBRAUN in the event of installation of parts manufactured by companies other than
MBRAUN which may result in additional and unknown hazards.
Page 2-3

Instruction Manual
Simultaneous operation of the system by two or more persons is not recommended as this may
cause hazards based on misconduct or mutual misunderstanding. In case of the system being
operated by two or more persons, operation should be conducted in such a way as to ensure
each individuals respective task does not influence other tasks in any way.
Safety covers, panels, panes, windows or doors may not be removed at any time, unless there
is a need for service. The system may not be opened (i.e. antechambers, etc.) during
processing or power failures. In the case of any safety deficiencies, the system must be
decommissioned and the service personnel informed accordingly. During decommissioning
compliance with all local health, safety and environmental guidelines must be followed.
2.7 Safety Electrical

This system operates under high voltage. Risk of injury caused by high voltages exists anytime
the system is connected to the power supply, this includes when the system is powered off.
Capacitors within the system may be charged when the system is switched off and
disconnected from main power supply.
Interchange of current bearing wires can result in electrical hazards
such as shock, involuntary muscle reaction, muscle paralysis, burnt
tissues and organs, or death.
Connection to the main power supply must be performed by a qualified electrician according to
local area guidelines. All neutral and ground wires must be connected accordingly.
Opening the system or removing parts when the system is powered on, may result in exposure
to live electrical connectors. Extreme caution should be taken to avoid directly or indirectly
touching live connectors to avoid possible electric shock.
Prior to performing any electrical service work on the system,
ensure the system is powered off and disconnected from the power
supply.
Service required while the system is in operation should only be performed only by qualified
personnel trained in the knowledge and prevention of all potentially dangerous and hazardous
situations.
The system must be grounded/earthed at all times. Do not remove or cut off any ground wire for
the system or its components. In case of insufficient grounding or damaged ground conductor
ensure the system will be inoperable and secure it against unauthorized or unintentional
operation.
Insufficient grounding can cause electrostatic charging of plastic
parts, hoses or pipes, wiring and/or the system as a whole, which
could cause solvents and process chemicals to ignite.
Replacement of fuses should be of the same type and current rating.
Makeshift fuses and/or short circuit fuse holders should never be
used in the operation of the system.
Page 2-4

Instruction Manual
2.8 Safety Handling of Electronic Components

Electrostatic discharges can cause damage to parts. When handling electronic components the
following precautions should be observed:
a) Wear a grounded wrist strap or work on a grounded static-dissipating work surface. If
this not possible touch an adjacent earth ground (i.e. central heaters or water pipes)
before handling electronic components or printed circuit boards.
b) Leave electronic components and printed circuit boards in their original packaging
until final installation.
c) Handle electronic components by their body or case, avoid touching of leads.
d) Keep electronic components and printed circuit boards away from such static
generating materials as vinyl, plastic bags, etc.
Maintenance and repair work required, but not listed in this manual,
should only be carried out by MBRAUN service or by persons of
equivalent qualification.
2.9 Safety Chemicals and Gases

Chemicals used in the system are not supplied by MBRAUN. Chemicals are provided and
applied by the system user.
Proper handling of chemicals, corrosives and solvents is the user's responsibility. Materials
used may be flammable, explosive, toxic.
Below are some guidelines to refer to when handling chemical substances:
Ensure the all relevant Control of Substance Hazardous to Health (COSHH) guidelines
are followed;
Observe relevant safety regulations as well as material safety data sheets (MSDS) and
additional advice provided by the supplier;
Wear proper protective safety masks, gloves and eyewear whenever working with
chemicals, corrosives or solvents;
Mark all containers and supply lines of chemicals (i.e. containers of media and waste)
with appropriate labels and warning signs;
Ensure proper ventilation and exhaustion of vapours;
Wear proper breathing protection to avoid the risk of suffocation;
Keep away from ignition sources;
Do not smoke;
Do not ingest food or beverage while working with the chemicals, corrosives or solvents
to avoid the risk of poisoning.
Released chemicals may react with each other, leading to
unwanted and/or unknown substances, which may cause additional
risks.
Proper handling of gases is the user's responsibility. Gases used may be flammable, explosive,
toxic. Below are some guidelines to refer to when handling gaseous materials:
Do not inhale the gas to avoid risk of suffocation.
Prevent electrostatic charging and beware of ignition sources.
Do not smoke.
When using corrosive, gassing or noxious materials, the safety of
all employees must be ensured by whatever means necessary.
Specifically, all employees must be trained in the safe handling of
the materials to be used.
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Instruction Manual
2.10 Safety Symbols Used on the System

The following symbols refer to MBRAUN components and parts. However, components and
parts of sub-suppliers may show other symbols, not expressly mentioned or referred to in this
manual. The following caution and command symbols may be seen on the system:
General hazard
Electrical hazard
Fire hazard
Toxic hazard
Explosion hazard
Pressurised gas hazard
Wear safety mask
Wear safety goggles
Wear protective gloves
The owner of the system is responsible to place adequate danger

signals and labels in suitable places. This applies in particular to
signals and labels concerning process chemicals used. Regardless
of the number of caution symbols and information placed on or
around the system, all safety instructions of this manual must be
observed!
2.11 Safety Emergencies

In case of an emergency, please observe the following instructions:
1. Immediately shutdown the system using the main power switch.
2. Disconnect the system from all gas supplies.
3. Refer to the material safety data sheets for information on treating the emergency.
Contact the appropriate emergency response personnel in the area and/or listed on the
material safety data sheets.
Do not disconnect the water supply for systems containing
components requiring a cooling water source.
Prior to restarting, the system must be fully checked for safety, contact the MBRAUN Service
Department after the emergency has been rectified.
In addition to the information contained in this manual all local
health, safety and environmental guidelines must be followed.
Page 2-6

Instruction Manual
2.12 Additional Information

The system is considered to be unsafe for operation if:
there is any visible damage;
it fails to perform according to specification;
it has been subject to prolonged storage under unfavourable conditions;
it has been subjected to severe transport stress.
If the system meets any or all of the above:
make it inoperable;
secure it against any unauthorized or unintentional operation;
contact the MBRAUN Service Department.
Do not perform any service or repair of the system or its
components other than described in this manual.
Maintenance, repair and service other than described in this manual
may only be performed by MBRAUN service personnel or properly
trained/qualified individuals.
Page 2-7
Chapter 3
3.1 Description of the System....3-1
Principles of Operation
3.2 Pressure Control System....3-1

3.3 Purging the System....3-2
3.4 Circulation Mode....3-3
3.5 Regeneration Mode....3-3
3.6 Antechamber Operation....3-4
3.7 Analyzers....3-5

Instruction Manual
Chapter 3: Principles of Operation
CHAPTER 3: PRINCIPLES OF OPERATION

3.1 Description of the System
M.Braun glovebox systems, which are operated using a closed loop circulation method, provide
the user with a non-reactive atmosphere of <1ppm moisture and oxygen content. The system
has three major functions: Purging, Circulation and Regeneration. Each of these processes is
explained in detail in the following sections. For information for operating the system functions,
refer to Chapter 9 of this manual.
3.2 Pressure Control System

General Information
The system is equipped with a PLC operated pressure control system which starts automatically
when the system is activated. Upper and lower working pressure parameters, which are set by
the user, provide a comfortable range for the user to work in. Depending upon the application,
the system can be operated in the positive or negative pressure state.
Definition of Terms
Box Pressure
Current pressure within the glovebox.
Working Range
The range within the working setpoints at which the box

pressure fluctuates.
Upper Working Setpoint

Lower Working Setpoint
Adjustable setpoint parameters used to establish the working

range.
Limit Setpoint
The fixed value range between which the working parameters

can be set. This is a factory established range which cannot be
adjusted.
Page 3-1

Instruction Manual
3.3 Purging the System

Purging the glovebox replaces the ambient air inside the box with the working gas by providing
a constant flow of working gas into and out of the box. The purge function can be performed
either manually by the user or automatically by the system. The automatic or quick purge
function is not included with all systems but is an available option that can be purchased at any
time after the glovebox has been installed and/or running for any period of time.
As a general guideline a system should be purged when the oxygen level inside the box
exceeds 100ppm. Possible reasons for an elevated oxygen level are:
Initial commissioning of the system;
Recent service or repair made to the system;
Room atmosphere entering the box due to improper use;
Leaks in the system or other damage (e.g. torn gloves, etc.).
The purging process can be performed three (3) different ways:
Reverse Purge
Performed on systems with no manual purge valve or automatic purge

function. This can also be performed on systems with either of the
mentioned options if the user chooses to do so.
The Reverse Purge method purges the working gas directly into the
users room environment, use caution when working in the vicinity of
a glovebox undergoing reverse purge.
Manual Purge
Performed on systems with a manual purge valve located on the top of

the glovebox system. This valve should be vented to a fume hood or
facility exhaust system.
Automatic Purge
Performed on systems with this function incorporated into the PLC

program via the VSA valve. This valve should be vented to a fume hood
or facility exhaust system.
Both reverse purge and manual purge procedures require the user to enter box pressure
setpoints in the PLC prior to beginning operation. The automatic purge function does not require
any parameters to be set prior to beginning the operation.
Purge time and gas consumption varies and is based on glovebox volume and the level of purity
required in the glovebox.
Figure 1 - Purge Gas Consumption
Figure 1 above shows that a desired box atmosphere purity of 10ppm requires approximately
14.50m of purge gas per cubic meter of box volume (refer to the Box Volume Table below):
Page 3-2

Instruction Manual
Box Volume Table
Box Length 1250
Box Length 1500
Box Length 1800
Box Depth 780
28.86 f3 / .81 m3
34.63 f3/ .98 m3
41.56 f3 / 1.18 m3
Box Depth 1000
35.51 f3 / 1.00 m3
42.61 f3 / 1.21 m3
51.13 f3 / 1.45 m3
Box Depth 1200
43.45 f3 / 1.23 m3
52.14 f3 / 1.48 m3
62.57 f3 / 1.77 m3
3.4 Circulation Mode

Circulation mode is the normal operation mode for working within the glovebox system. During
normal operation working gas is continuously circulated between the glovebox and the gas
purification system. As the working gas passes through the filter column the oxygen and
moisture content is scrubbed from the gas. This provides the user with a consistent inert
glovebox environment in which to work.
Figure 2 - Principle of Circulation
3.5 Regeneration Mode

When the glovebox is used for an extended period of time, oxygen and moisture begins to
accumulate inside the purifier filter column which decreases the effectiveness of the circulation
process. Regeneration is the process of removing the excess oxygen and moisture from the
filter bed material inside the purifier column. This is a multi-step 16 hour process during which
the glovebox cannot be used for normal operation (circulation).
The regeneration procedure should not be interrupted at any time
during the process. If for any reason the process is terminated
prematurely, do not restart any system processes. Contact the
M.Braun Service Department immediately.
Page 3-3

Instruction Manual
3.6 Antechamber Operation

General Information
The system is equipped with a large (and/or small) antechamber designed for transferring
material into or out of the glovebox without polluting the internal box atmosphere or the room
atmosphere. The antechamber(s) is equipped with an inner door, outer door and sliding tray to
ease in transferring materials.
Transferring material into and out of the box can be done in the Automatic or Manual Modes.
Both modes utilize the antechamber components and the touch screen. The chamber
undergoes several evacuation/refill cycles to clear the chamber of room or glovebox
atmosphere prior to removing the material from the chamber.
An evacuated chamber cannot be opened. Attempting to open an
evacuated antechamber may damage the door locking
mechanism.
Keep both antechamber doors closed when not transferring
materials.
Method of Operation
Open outer door and transfer material into antechamber
Close outer door
Refill antechamber with box gas

Evacuate antechamber
Repeat the Evacuate and Refill antechamber

steps according to the guidelines listed in
Chapter 9 Section 9.4 Antechamber Operation.
Open inner door and transfer material into glovebox
Page 3-4

Instruction Manual
Important Notes
Never open the inner and outer antechamber doors
simultaneously.
Never open the inner door of an antechamber filled with room
atmosphere. This would result in pollution of the box atmosphere
which could cause damage to instruments and/or materials
inside the box.
Depending on the materials used inside the glovebox, vapors
may be released into the room when material is transferred into
or out of the glovebox.
Material transferred between the glovebox and room atmosphere
must be able to withstand the pressure difference during the
antechamber purge process (e.g. partially filled vessels, etc.). Open
the seal slightly (e.g. lids of bottles, etc.) to allow the vessel to be
vented to prevent implosion.
3.7 Analyzers
General Information
This section applies only to systems that are equipped with oxygen and/or moisture analyzers.
Each analyzer contains a sensor head that is protected with a metal cap and electronics that are
used to measure (depending on the analyzer type) the oxygen or moisture content of the
working gas inside the glovebox. The analyzers are connected to the PLC via a cable with an
RJ45 connector.
While not all systems are equipped with one or both analyzers at the time of initial installation
they can be purchased at any time. Please contact the M.Braun Sales or Service Department
for further information.
Oxygen Analyzer (MB OX-SE-1)

The oxygen analyzer, with a measuring range of 0
1,000ppm, monitors the atmosphere inside the glovebox for
residual oxygen content. The analyzer is designed to provide
exact readings within the range of 0 100ppm. An estimated
reading of the oxygen content is provided when the oxygen
level rises above 100ppm.
The sensor element is a miniaturized Zirconium Dioxide plate
operated at elevated temperature that is controlled by a
platinum resistor. The electronics are supplied with 24 V DC
and deliver a 0 10V signal proportional to the concentration
of Oxygen. An additional input for the electronics allows the
switching on and off of the sensor heating by means of the
PLC.1
Contact with Hydrogen, solvents and other aggressive gaseous
substances will cause permanent damage to the sensor.
M.Braun, Inc., Sales Literature, MB OX-SE1 Oxygen Probe, (Edition 2/2004)
Page 3-5

Instruction Manual
Moisture Analyzer (MB MO-SE-1)
The moisture analyzer, with a measuring range of 0
350ppm, monitors the atmosphere inside the glovebox for
residual moisture content. The analyzer is designed to
provide exact readings within the range of 0 50ppm. An
estimated reading of the oxygen content is provided when the
oxygen level rises above 50ppm.
The sensor element consists of two (2) parallel platinum coils
coated with phosphoric acid, and a PT1000 temperature
sensor. The electronics are supplied with 24VDC and deliver
a 0 10V signal in proportion to the concentration of H20.2
M.Braun, Inc., Sales Literature, MB MO-SE1 Oxygen Probe, (Edition 2/2004)
Page 3-6
Chapter 4
Touch Panel Operation 4.2 Input and Password Fields....4-1

4.3 Displaying the System Information....4-2

Instruction Manual
Chapter 4: Touch Panel Operation
CHAPTER 4: TOUCH PANEL OPERATION

The glovebox system utilizes a Touch Screen Display panel. All functions and/or selections are
performed by lightly touching the button or icon on the screen.
An optional protective self adhesive foil is available for the touch

screen. This protects the screen from scratches, dirt and grime.
This protective foil also provides a matte surface to reduce glare
and can be removed at any time without leaving any residue on the
screen.
Do not use any sharp or pointed objects to remove the protective
foil as this may result in damage to the touch screen.
4.2 Input and Password Fields

Input Fields
Certain screens contain information which can
be changed by the user. This information is
entered using an alphanumeric pad which is
displayed when an input field is touched.
When an input field is activated, a keyboard is
displayed on the screen.
Using this keyboard, you can enter new values
into an input field.
After completing the entry, press the enter key to

confirm the value.
Password Fields
Certain input fields require a user password to be
entered. When an input field requiring a password is
touched, a password screen is displayed. To enter the
password, touch the white area in the screen. Only
authorized users can make changes to the input fields.
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Instruction Manual
4.3 Displaying the System Information

Touch the Common Parameters button on
the Start Screen.
Touch the System button to access the

System settings screen.
Touch the Info button.
Page 4-2

Instruction Manual
The Information Screen is displayed
providing M.Braun contact information and
information regarding the project number or
serial number for the system.
Page 4-3
Chapter 5
System Screens
5.2 Start Screen....5-1

5.3 Common Parameters Screen....5-3
5.4 Alarm Setpoints Screen....5-4
5.5 Min./Max. Values Screen....5-4
5.6 Functions Screens....5-5
5.7 Antechamber Screen....5-7
5.8 Antechamber Parameters Screen....5-8
5.9 Antechamber Trends Screen....5-9
5.10 Box Pressure Parameters Screen....5-9
5.11 Purifier Layout Screen....5-10
5.12 Purifier Parameter Screen....5-11
5.13 Purifier Status Screen....5-11
5.14 Operating Hours Screen....5-12
5.15 Purifier Trends Screen....5-12
5.16 System Screen....5-13
5.17 Box Purging Screen....5-13
5.18 Freezer/Box Cooling Parameters Screen....5-14
5.19 Warning Screen....5-11

Instruction Manual
Chapter 5: System Screens
CHAPTER 5: SYSTEM SCREENS

The system is operated using a Touch Screen located on the side of the system above the
antechamber. This chapter provides an introduction to the various screens that the user will
access during operation of the system.
To activate a component or operation touch the corresponding button. To deactivate a
component or operation touch the corresponding button again.
Red buttons and icons represent inactive functions, green buttons and icons represent active
functions and gray buttons represent functions which are nonexistent or temporarily unavailable.
The purifier icon will also be displayed in yellow signaling the regeneration process is active.
The list below displays the buttons and information fields which are located on multiple screens
associated with the system.
Button / Information Field
Description
Displays the Alarm Screen. Blinks black & red when an alarm
is present.
Displays the actual glovebox pressure.
Displays the moisture level inside the glovebox as detected by
the analyzer.
Displays the oxygen level inside the glovebox as detected by
the analyzer.
Displays the previous screen.
Displays the Start screen.
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Instruction Manual
5.2 Start Screen

Main screen displayed on the touch
panel.
All functions and screens related to the
operation of the glovebox and other
components are accessed via the Start
Screen.
Icon / Button
Description
Displays the status of the blower.
Displays the status of the vacuum pump.
Purifier
Inactive
Circulation
Active
Regeneration
Active
Displays the status of the purifier.

Used to access the Purifier Layout screen.
Displays the current pressure inside the glovebox.
Displays the Moisture and/or Oxygen levels inside the
glovebox as determined by the analyzer(s) when present.
Touching any area in this box will display the Box Pressure
Parameters Screen.
Displays the actual freezer temperature. This field is not
displayed when there is no freezer present on the system.
Page 5-2

Instruction Manual
5.3 Common Parameters Screen

Accessed via the Start Screen by
touching the Common Parameters
button.
Button
Description
Displays the Alarm Setpoints screen.
Displays the analyzer Min./Max. Values screen.
Displays the Operating Hours screen.
Displays the Box Trends screen.
Displays the System information screen.
Displays the Service screen.
Displays the Box Purging Screen.
Displays the Freezer/Box Cooling parameters screen.
Page 5-3

Instruction Manual
5.4 Alarm Setpoints Screen

Accessed via the Common Parameters
screen by touching the Alarm Limits
button.
Button
Description
Used to set the alarm parameter for the
moisture analyzer when present on the
system.
oxygen analyzer when present on the
system.
freezer when present on the system.
Used to set the alarm parameter for the box
cooling unit when present on the system.
5.5 Min./Max. Values Screen

screen by touching the Min./Max. Values
button.
This screen displays the highest and
lowest oxygen and moisture levels as
detected by the analyzers.
Use the Reset button to reset the
displayed values to zero.
Page 5-4

Instruction Manual
5.6 Functions Screens

Not all functions shown on this screen are included with every
system. In the event of a component or function not being included,
the button will not be displayed on this screen.
Functions Screen 1
Accessed via the Start Screen
touching the Functions button.
by
Circulation Purifier and Regeneration

Purifier 2 buttons are permanently grayed
out for single purifier systems.
Button
Description
Used to activate and deactivate the
circulation process.
Used to activate the regeneration
process.
vacuum pump.
analyzer(s). (This button is grayed out
when analyzers are not present on
the system.)
Used to activate the automatic purge
process.
Used to turn the light on and off.
Used to turn the freezer on and off.
(This button is only present when
there is a freezer on the system.)
Page 5-5

Instruction Manual
Functions Screen 2
Accessed via Functions Screen 1 by
touching the Next button.
Antechamber 2 buttons are permanently
grayed out when only one large
antechamber is present on the system.
Button
Description
Used to start an automatic antechamber cycle for
the large antechamber.
Used to evacuate the large antechamber in
manual mode.
Used to refill the large antechamber in manual
mode.
Used to activate the vacuum pump for the
antechamber. (This is activated automatically
when the antechamber is operated using the
purifier vacuum pump.)
Page 5-6

Instruction Manual
5.7 Antechamber Screen

Accessed via the Start Screen
touching the Antechamber icon.
by
This screen is used to operate the large

antechamber only.
Information Field / Icon / Button
Description
Displays the active step number and step description
when the system is being run.
Identifies which mode the system is being run in.
Displays the actual pressure inside the chamber.
Displays the status of the pressure level inside the
antechamber.
Displays the status of the refill valve.
Displays the status of the evacuation valve.
Used to activate and deactivate the various components

and processes. These buttons work the same as the
buttons located on the Functions screen.
Used to access the antechamber parameters screen.
Used to access the antechamber trends screen.
Used to operate the antechamber in Automatic Mode.
Used to operate the antechamber in Manual Mode.
Page 5-7

Instruction Manual
5.8 Antechamber Parameters Screen

Accessed via the Antechamber Screen
by touching the Parameters button.
Information Field / Icon / Button
Description
Level at which the antechamber refills during
the automatic evacuation/refill cycle.
The level at which a leak test is performed on
the antechamber in automatic mode.
Level at which the refill process of the
antechamber is initiated in automatic mode.
Number of intermediate refill cycles performed
in automatic mode.
The maximum amount of time allowed for the
antechamber to evacuate to the vacuum set
point.
Level at which the system alarm will display,
above the vacuum leak test set point value.
For example if the setpoint is 5x10-1 mbar and
the maximum leak rate value is 3, the alarm
will not display until the set point reaches
8x10-1 mbar.
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Instruction Manual
5.9 Antechamber Trends Screen

Accessed via the Antechamber Screen by
touching the Trends button.
Use the various buttons on the screen to scroll
through the designated time period and also to
zoom in on a selected range over a 24 hour
period.
5.10 Box Pressure Parameters Screen

touching the Box Pressure and
Analyzer Details field.
Information Field
Description
Used to enter the upper working box pressure setpoint.
Used to enter the lower working box pressure setpoint.
Displays the setpoint at which the box pressure will rise

or drop when the actual box pressure reaches the upper
or lower working limit.
Page 5-9

Instruction Manual
Information Field
Description
Displays the default pressure alarm limits for the system.
Displays the Freezer/Box Cooling screen.
5.11 Purifier Layout Screen

touching the RKM icon.
Valve Definitions
VHE1 - Purifier Inlet
VHA1 - Purifier Outlet
VRE1 - Regen. Gas Inlet
VRS - Regen. Gas Outlet
VS1/2 - Pressure Compensation
VG - Working Gas Inlet
VRV - Regen. Vacuum
VV - Box Vacuum
VPG - Vacuum Pump
Icon / Information Field / Button
Description
Displays the status of the various valves throughout the
system.
Displays the status of the blower.
Displays the status of the purifier.

Purifier
Inactive
Circulation
Active
Regeneration
Active
Displays the active step of the regeneration process.

Used to activate and deactivate the various components
and processes. These buttons work the same as the
buttons located on the Functions screen.
Used to access the purifier parameters screen.
Page 5-10

Instruction Manual
5.12 Purifier Parameter Screen

Accessed via the Purifier Layout screen
by touching the Parameters button.
Choose Yes to have circulation start
automatically after the regeneration
process is completed.
Choose No to start circulation manually
after the regeneration process is
completed.
5.13 Purifier Status Screen

Accessed via the Purifier Parameter
screen by touching the Status button.
Button / Information Field
Description
Displays the current mode being used to operate
the system.
Displays the time remaining
regeneration process is complete.
until
the
Displays the number of hours the system has

been operating in circulation mode since the last
regeneration process was performed.
Displays the total hours the purifier has been in
operation.
Page 5-11

Instruction Manual
5.14 Operating Hours Screen

screen by touching the Operating
Hours button.
Displays the number of hours which the
components have been running.
Under normal use the vacuum pump oil should be changed every
3,000 hours per the manufacturers recommendation.
5.15 Purifier Trends Screens

screen by touching the Trends button.
Use the various buttons on the screen to
scroll through the designated time period
and also to zoom in on a selected range
over a 4 hour period.
Touch the 24h button to change the
display range to a 24 hour period.
Page 5-12

Instruction Manual
5.16 System Screen

screen by touching the System button.
Used to display system information or
perform maintenance on the touch
screen.
5.17 Box Purging Screen

screen by touching the Box Purging
button.
Information Field
Description
Used to automatically purge the glovebox
when the oxygen level reaches the Oxygen
Limit Value setpoint entered below.
Value at which the purging process will initiate
when the above field is set to Yes.
Used to automatically initiate the purge
procedure when the box pressure reaches the
factory established Lower Limit Setpoint.
Page 5-13

Instruction Manual
5.18 Freezer/Box Cooling Parameter Screen

screen by touching the Freezer Box
Cooling button. This screen is also
accessed via the Box Pressure
Parameters by touching the More
button.
This screen applies only to systems with
a freezer and/or box cooling unit.
Information Field
Description
Used to set the freezer operating temperature.
Displays the actual temperature inside the
freezer.
Used to set the box cooling unit operating
temperature.
Displays the actual temperature inside the
glovebox.
5.19 Warning Screen

Accessed via any screen by touching the
Alarm button when displayed.
This screen lists the:
Warning number;
Time the error occurred;
Description of the error.
Depending upon the type of alarm, the
message:
Will disappear upon fixing the error;
Must be acknowledged by touching
the ACK button after the error has
been fixed before it will disappear.
Page 5-14
Chapter 6
6.1 Location....6-1
Installation
Requirements
6.2 Gas Supply and Connection Requirements....6-1

6.3 Venting Requirements....6-2
6.4 Electrical Connection Requirements....6-2
6.5 Water Cooling Requirements....6-2

Instruction Manual
Chapter 6: Installation Requirements
CHAPTER 6: INSTALLATION REQUIREMENTS

6.1 Location
The room in which the glovebox will be used should be dry and well ventilated with a
temperature between 59F and 86F (15C to 30C). The floor should be level and stable
enough to support the weight of the glovebox system.
Position the glovebox in an area that allows the user to have adequate working space in and
around the system (e.g. gloveports, antechamber(s), etc.). If positioned against or near a wall,
the clearance should be a minimum of approximately 24 inches (600 mm) for the rear and side
of the glovebox without antechamber(s).
6.2 Gas Supply and Connection Requirements

Working Gas
Use:
To establish and maintain the ultra pure gas atmosphere. Used as the
operating gas for electropneumatic valves.
Gas Type:
Nitrogen, Argon, Helium supplied at 80psi.
Purity:
Industrial grade or better; from tanks or other facility gas supply source.
Quantity:
Permanent supply for the systems operation (e.g. pressure compensation,

etc.).
Regulator Type:
Two (2) stage with a 0 100psi gauge.
Pressure:
80psi
Flow rate:
200 liters/minute
Material:
3/8 Braided Tygon hose (supplied with the system).
Connections:
male NPT to 3/8 O.D. hose barb (connects Tygon hose to gas
regulator).
Regeneration Gas
Use:
Reprocessing saturated purifier columns.
Gas Type1:
Nitrogen as the Working Gas: 90-97% Nitrogen with 3-10% Hydrogen

balance
Argon as the Working Gas: 90-97% Argon with 3-10% Hydrogen balance
Helium as the Working Gas: 90-97% Helium with 3-10% Hydrogen balance
Quantity:
Approximately 3,500 liters for each regeneration process.
Regulator Type:
Two (2) stage Hydrogen regulator with a 0 20psi gauge.
Pressure:
5psi
Flow Rate:
15 20 liters/minute
Material:
3/8 Braided Tygon hose (supplied with the system).
Connection:
male NPT to 3/8 O.D. hose barb (connects Tygon hose to gas
regulator).
Gas combination will vary depending upon the type of working gas used based on users application.
Page 6-1

Instruction Manual
Chapter 6: Installation Requirements
6.3 Venting Requirements

Venting to the glovebox system and vacuum pump to the facility ventilation exhaust system is
not mandatory but is recommended.
Vacuum Pump:
1 inner diameter braided Tygon (or similar type) hose.
Regeneration Exhaust Gas:
3/8 inner diameter braided Tygon (or similar type) hose.
Purge Valve :
1 inner diameter braided Tygon (or similar type) hose.
This is an optional item which is not included with all systems.
This applies to both automatic and manual purge valve types.
6.4 Electrical Connection Requirements

The power supply for the system will vary according to local area guidelines. Please refer to the
system label located on the side of the box in the upper right hand corner above the
antechamber for information specific to the system.
Type NEMA 5-20R non GFCI outlet(s) are required for all electrical
connections.
6.5 Water Cooling Requirements

The glovebox system includes a heat exchanger which is required to be connected if the box
atmosphere is to be cooled.
Temperature:
15C - 25C
Flow Rate:
2 liters/minute at 10C, 5 liters/minute at 15C
Inlet Pressure:
2.0 4.0 bar
Outlet Pressure:
Depressurized (maximum 0.5mbar)
Material:
3/8 Braided Tygon (or similar type) hose.
Page 6-2
Chapter 7
Installation
7.2 Tools and Materials Required....7-1

7.3 Assembling the Glovebox....7-3
7.4 Connecting the Antechamber Gauges and Sensors....7-3
7.5 Electrical Feedthorugh and Power Strip....7-4
7.6 Stand Mounted Purifier.....7-6
7.7 Stand Alone Purifier....7-8
7.8 Attaching the Vacuum Pump Oil Mist Filter....7-11
7.9 Attaching the Gloves....7-12
7.10 Connecting the Freezer and Compressor....7-13
7.11 Attaching the Analyzers....7-14
7.12 Lowering the Leveling Feet....7-15
7.13 Power and Gas Supply....7-16

Instruction Manual
Chapter 7: Installation
CHAPTER 7: INSTALLATION
The M.Braun glovebox has been fully assembled, operated and tested at our production facility.
The information binder contains the:
Glovebox Project Checklist Displays detailed information regarding the glovebox

system
Test Sheet displays the various operations and components which were tested during
production
Product Identification and Traceability Lists all serial numbers for the components
included on the system
Vendor Component Manuals Operating instructions, etc. for components included on

the system which are not manufactured by M.Braun.
The glovebox and antechamber(s) have been fully tested for leaks under positive and negative
pressure. The vacuum level during the regeneration process has also been tested to ensure
that the glovebox purifier performs optimally.
This chapter provides information regarding the actual assembly of the glovebox prior to use. All
instructions included in this chapter should be strictly adhered to. Any questions that arise
during the installation process should be directed to the M.Braun Service Department.
The Unilab system comes complete and ready to assemble. The components, which have been
numbered for ease in assembly, should be installed in the order listed in these instructions not
in the order which they are numbered.
All clamp and o-ring assemblies used to

attach pipes and hoses should be tightened
finger tight.
Prior to tightening the clamp onto the flange,
ensure the o-ring and other components are
seated properly to avoid leaks.
Figure 1 - Clamp, Flange, O-Ring Assembly
7.2 Tools and Materials Required

Tools (not supplied by M.Braun):
13mm Wrench
19mm Wrench
Flathead Screwdriver
18mm wrench
Adjustable Wrench
Page 7-1

Instruction Manual
Materials:
The quantity for each material or component listed below will vary depending upon the length
of the system (i.e. 1250/780, 2500/1000, etc.). Please refer to the checklist included with the
system for exact quantities.
Gloves
KF25 Clamp
KF40 Clamp
Glove O-ring
KF25 O-ring
KF40 O-ring
Electrical Feedthrough
Power Strip
3/8 Braided Tygon Tubing
KF25 Stainless Steel

Flexible Hose
1 Pipe
(copper or stainless steel)
Hose Clamp
Vacuum Pump
Oil Mist Filter

Quantity (1) per vacuum pump
Funnel O-ring
Quantity (1) per vacuum pump
Oil
Quantity (1) bottle per vacuum pump
O2 Analyzer
Optional Item
U-shaped clamp, O-ring, Bolt and Thumb Screw for

Oil Mist Filter
Quantity (1) each per vacuum pump
H2O Analyzer
Optional Item
Manual Purge Valve

Optional Item
Page 7-2

Instruction Manual
7.3 Assembling the Glovebox

General Information
A packing checklist has been included with the system. Most components included on this
checklist have been packed in the antechamber. Prior to beginning the installation process,
unpack, unwrap and identify each component and ensure the appropriate number of items has
been included with the shipment as indicated on this checklist. Contact the M.Braun Service
Department if any of the items are missing or damaged.
Place all components in an easily accessible location close to the glovebox in an organized
manner to ease in the assembly process.
This chapter contains assembly information for the Labmaster 130 (stand mounted purifier)
and MB20G (stand-alone) style gloveboxes. Please refer to the pictures below to determine the
type of system and follow the corresponding assembly instructions.
Figure 2 - Stand Mounted Purifier
Figure 3 - Stand-Alone Purifier
Section 7.6 provides instructions for installing a Stand Mounted purifier glovebox system.
Section 7.7 provides instructions for installing Stand-Alone purifier glovebox systems. Sections
7.4-7.5 and 7.8 through 7.13 provide instructions which pertain to both types of glovebox
systems.
Prior to beginning the installation process move the system into the permanent location but
allow enough clearance during the installation process to work behind the box and/or purifier.
These installation instructions include all possible components for a
glovebox system. Please note, not all components are included
with every system.
7.4 Connecting the Antechamber Gauges and Sensors

The gauges and sensors depicted below will depend on the options purchased with the system.
Please refer to your Project Checklist for more information.
The gauges are attached with KF16 clamps and o-rings and the electrical connections are made
by attaching the corresponding labeled plug from the system to the gauge and/or sensor. The
picture below provides information for the various gauges and the completed assembly.
Page 7-3

Instruction Manual
B
A
Figure 4 Antechamber Valve, Gauge & Sensor Assembly
A.
B.
C.
D.
Evacuation Valve (VSV) Standard on an LM130 and optional on an MB20G

Refill Valve (VSF) Standard on an LM130 and optional on an MB20G
Pirani Gauge (GP114) Present when auto antechamber control option is included.
Rough Gauge (GP113) Present when auto antechamber control option is included.
7.5 Electrical Feedthrough and Power Strip

There are multiple flanges located on the back of the glovebox which are used to attach
feedthroughs to allow components to be used inside the glovebox. Any one of these can be
used for the supplied electrical feedthrough.
Select one feedthrough that is close to the

main power supply (outlet) and remove the
clamp, blank cap and o-ring.
Place the o-ring on the female connector side

of the feedthrough.
Page 7-4

Instruction Manual
Place the female connector end of the

feedthrough into the flange and feed into the
box until the feedthrough flange is flush with
the box flange.
Place the clamp around the flange and tighten.
Place the 6 outlet power strip inside the

glovebox through one of the gloveports located
on the front of the box.
Plug the power strip into the feedthrough.
Page 7-5

Instruction Manual
7.6 Stand Mounted Purifier

Systems with stand mounted purifiers only require the vacuum pump and other miscellaneous
components to be connected prior to use. Please refer to sections 7.7 through 7.<> for
instructions on connecting the other components pertaining to the system.
Connecting the Vacuum Pump
Attach the KF25 stainless steel flexible hose

to the end of the 1 pipe with the port using a
KF25 clamp and o-ring.
Attach the pipe to the valve located on the

bottom of the rear side of the antechamber
using a KF40 clamp and o-ring.
Attach the stainless steel flexible hose to the

port on the vacuum pump with the funnel
shaped o-ring using a KF25 clamp.
Attach the fitting on the purifier vacuum hose

(H) to the port on the copper or stainless pipe
and tighten hand tight.
Using the 19mm and 18mm wrenches
Tighten an additional turn.
Page 7-6

Instruction Manual
If system is
antechamber:
equipped
with
mini
Attach the end of the vacuum hose (H) with

the elbow to the port located on the rear side
of the mini antechamber.
Use care not to cross thread brass to brass connections and

stainless steel to stainless steel connections when fitting together
and tightening.
7.7 Stand Alone Purifier

Below is a rear view of the purifier showing the various components and cables which require
connection to the glovebox. Please refer to these pictures when following the instructions.
B
A
C
I
G
F
Figure 5 - Purifier Layout
A.
B.
C.
D.
E.
F.
G.
H.
I.
J.
D
Figure 6 - Completed Purifier Setup
Purifier Cart
PLC-Touch Screen Panel and Housing
Inlet Hose from Glovebox to Purifier
Outlet Hose from Blower to Glovebox
Lighthood Cable
Pressure Sensor Cable
Ground Wire
Freezer Connection Cable
Purifier Vacuum Hose
Main Power Cable
Foot Pedal
Page 7-7

Instruction Manual
Connecting the Purifier
After the purifier is attached, it is possible to move the glovebox
forward and backward (to allow additional connections to be made)
but side to side will be extremely difficult. Therefore, it is
recommended that prior to connecting the purifier, the glovebox be
moved into the final location where it is to be used.
Roll the purifier cart into place under the antechamber.

The recommended distance between the front leg of
the stand and the electrical cabinet is 2 3.
Remove the bolt from the black PLC bracket located

on the end panel where the antechamber(s) is located.
Place the hinge side of the touch panel enclosure

between the openings of the bracket.
Affix the enclosure to the bracket using the bolt, by
guiding it down and through the holes in the bracket
and hinge.
Page 7-8

Instruction Manual
Secure the hinge by using the 13mm and adjustable

wrenches.
Attach the KF40 stainless steel flexible hose (B) from

the VHE valve on the filter column to the circulation
pipe using a KF40 clamp and o-ring.
Attach the Out KF40 stainless steel flexible hose (C)

on the blower to the bottom of the heat exchanger
using a KF40 clamp and o-ring.
Connecting the Ground Wire
Using the 13mm wrench remove the

grounding bolt located on the inside of the
rear leg of the stand adjacent to the purifier
cart by turning in a counterclockwise motion.
Page 7-9

Instruction Manual
Remove the two bottom washers from the

bolt.
Place the end of the ground wire (F) onto the
bolt.
Place the washers onto the bolt with the

toothed washer teeth pointing towards the
leg of the stand.
Screw the bolt back into the leg by turning in

a clockwise motion.
7.8 Attaching the Vacuum Pump Oil Mist Filter
Place the black o-ring onto the remaining

port on the vacuum pump.
Page 7-10

Instruction Manual
Place the oil mist filter onto the port.
Push the u-shaped clamp onto the port

making sure it covers both the port and
filter flange.
Turn the clamp so the open ends face

out.
Secure in place with the bolt and red
thumb screw. Tighten hand tight.
Turn the filter so the label faces the gas

ballast on the top of the vacuum pump.
Push the black hose from the pump onto
the port on the filter. Secure in place with
black plastic clamp.
7.9 Attaching the Gloves

Slide the glove onto hand and insert arm into
the box through the gloveport angling the
thumb to a comfortable working position.
Page 7-11

Instruction Manual
Slide hand out of the glove and work the cuff

of the glove into the groove in the gloveport
closest to the glass. At this time the thumb
position can still be adjusted by rotating the
cuff of the glove.
Secure the glove with two o-rings.

Place the o-ring in the top of the second
groove from the glass and in one smooth
motion pull down across the circumference
of the gloveport.
Repeat the process for the remaining glove(s)
and o-ring(s).
7.10 Connecting the Freezer and Compressor

General Information
This section applies only to systems that are equipped with freezers.
Remove the cover from the horizontal wire

track located on the rear of the stand.
Page 7-12

Instruction Manual
Run the freezer connection cable (G) along
the inside of the wire track.
Place the end of the cable on the stand and
replace the wire track cover.
Connect the power supply plug for the

compressor.
The connector type will vary from system to system. The picture
shown above displays the two possible connector types that will be
used. Contact the M.Braun Service Department with any questions.
7.11 Attaching the Analyzers

General Information
This section applies only to systems that are equipped with analyzers.
Carefully place a KF40 o-ring on the analyzer

port.
Carefully place the sensor end of the analyzer

into the corresponding port on the circulation
piping running the length of the system on the
underside of the glovebox.
H2O analyzer is positioned on the side of the
box opposite the antechamber(s).
O2 analyzer is positioned on the same side of
the box as the antechamber(s).
Page 7-13

Instruction Manual
Secure the analyzer to the pipe using a KF-40

clamp and o-ring.
Repeat this process with the remaining analyzer(s).
Insert the analyzer cable plug into hole on the

corresponding analyzer by pushing the plug
into the hole gently until it snaps into place.
The Green plug attaches to the H2O Analyzer.
The Blue plug attaches to the O2 Analyzer.
The green plug cable should be wrapped along the circulation pipe
and then plugged into the moisture analyzer.
7.12 Lowering the Leveling Feet

Move the system into its permanent location.
Lower each of the leveling feet by hand until
they touch the ground.
Page 7-14

Instruction Manual
Using a 13mm wrench continue to lower the
leveling feet until the casters are raised off the
ground.
7.13 Power and Gas Supply

Plug the main power cable (I) on the purifier into the main power supply outlet.
Attach the working gas line from the purifier to the working gas supply.
Attach the regeneration gas line from the purifier to the regeneration gas supply.
Place the foot pedal on the floor in front of the system in an easily accessible location.
The installation is now complete.
Page 7-15
Chapter 8
Commissioning the System 8.2 System Activation....8-1

8.3 Static Pressure Test....8-2
8.4 Purging the System....8-4

Instruction Manual
Chapter 8: Commissioning the System
CHAPTER 8: COMMISSIONING THE SYSTEM

After completing the installation, the system is ready to be activated. Gas can now be added to
the box to prepare for normal operation. The system is equipped with a foot pedal which is used
during a pressure leak test or during normal operation of the box to temporarily increase or
decrease the pressure inside the glovebox.
While a leak test has been performed during production of the system, after initial installation
and gas has been added, it is necessary to perform a static pressure test to check the system
for any possible leaks. This is done to ensure all connections made during the installation are
tight and seated properly. The static pressure test also checks other components on the system
which may have shifted during the shipping process.
After the leak test has been completed, the purge operation must be performed to rid the box of
all room air and replace it with the chosen working gas. After the purge procedure is complete,
the box can be used for normal operation in circulation mode.
8.2 System Activation

Turn the Main Power switch located on the side of
the electrical cabinet on the purifier cart from the Off
position to the On position.
The panel will display a series of screens (program
starting up) before the start screen is displayed.
Touch the Functions button on the start screen.
Touch the Vacuum Pump button to activate the

vacuum pump.
Touch the Box Light button to turn the light on.
Page 8-1

Instruction Manual
The vacuum pump should remain on at all times while the system is
in operation.
Touch the End button to return to the start screen.
Using the regulator, turn on the working gas supply and set the pressure to 80psi.
8.3 Static Pressure Leak Test

The static pressure leak test is performed in both the positive and negative pressure states. The
process for running the test in either state is the same with the exception of the parameter
setpoints and foot pedal controls.
Touch the Box Pressure/Analyzer display box on

the start screen.
Use the arrows listed under Upper Working Limit

and Lower Working Limit to increase or decrease
the box atmosphere pressure setpoint in one tenth
decimal intervals.
or
Touch the number field next to the corresponding

parameter to display the alphanumeric keypad.
Recommended Positive State Setpoints
Upper Working Limit: 14.0mbar
Lower Working Limit: 0.0mbar
Recommended Negative State Setpoints
Upper Working Limit: -14.0mbar
Page 8-2

Instruction Manual
Enter the desired limit and touch the
button to return to the Box pressure screen.
button to set the parameter to the
Use the
positive or negative.
Touch the End button on the Box Pressure

Parameters screen.
Using the foot pedal control, adjust the gas pressure inside the box until the pressure reaches
the desired level range.
The pressure will fluctuate until it settles at a fixed level between +/- 10-12mbar.
If the pressure drops below +/-10mbar, use the foot pedal to add
gas until the pressure reaches the desired level.
Once the pressure settles, monitor the pressure level for five (5) minutes.
It is normal for the pressure level to fluctuate slightly during the 5
minute static pressure test. However, a pressure decrease of more
than .3mbar indicates there is a leak in the system. Check all
connections made during the installation process. If this doesnt fix
the problem, refer to the Troubleshooting chapter of this manual or
contact the M.Braun Service Department.
After five (5) minutes, if the pressure level has stayed constant, the glovebox can be tested in
the opposite pressure state.
After testing the box in both the positive and negative states with no leaks present, the box is
ready for purging.
Page 8-3

Instruction Manual
8.4 Purging the System

Systems equipped with freezers or other components that may be
protected by covers must be open during the purging process.
If the system has been in use prior to purging, ensure that the
freezer(s) is deactivated and is at room temperature before
beginning the process.
The instructions below include information regarding glovebox systems equipped with
analyzers. The analyzers, which are used to monitor the oxygen and moisture levels inside the
glovebox, can be used to determine when the glovebox atmosphere reaches the desired purity
level.
Glovebox systems which are not equipped with analyzers require the gas consumption to be
monitored to determine when the glovebox atmosphere reaches the desired purity level. Refer
to the Gas Consumption chart in Chapter 3 for more information.
Box Purge Volume Table

Box Length 1250
Box Length 1500
Box Length 1800
Box Depth 780
13,000 liters
14,000 liters
18,000 liters
Box Depth 1000
14,000 liters
18,000 liters
22,000 liters
Box Depth 1200
18,000 liters
22,000 liters
26,000 liters
Adjusting the Parameters

Adjusting the parameters is only necessary when using the
Reverse or Manual purge methods.

the start screen.
Page 8-4

Instruction Manual
decimal intervals.
or
parameter to display the alphanumeric keypad. Use
the keypad to adjust the parameters in the same
manner as before.
Recommended Setpoints
Upper Working Limit: 12.0mbar
Touch the End button.
Reverse Purge Method (no manual purge valve or quick purge function present)
Open the inner antechamber door.
Very slowly turn the outer antechamber door handle

in a counterclockwise motion until the glovebox
pressure drops to a level between 1.0mbar and
8.0mbar and the VG valve stays open* providing a
constant flow of gas into and out of the box.
*When the valve stops clicking it is open.
Page 8-5

Instruction Manual
Manual Purge Method (manual purge valve present)
Open the manual hand valve located on the top of
the glovebox.
The VG valve will open and close to allow working gas to flow into the box. Once the pressure
inside the box drops to a level between 1.0mbar and 8.0mbar, the valve will stay open*
providing a constant flow of gas into and out of the box.
*When the valve stops clicking it is open.
Automatic Purge Method (quick purge function present)
Touch the Quick Purge button on the functions

screen to activate the purging process.
The circulation button is blanked out as this function
is unavailable during the purging process.
Continue purging for 45 minutes.
Touch the
analyzers.
Analyzer
button
to
activate
the
Touch the End button to return to the Start Screen.
Continue purging until the oxygen and moisture content inside the box reach the desired ppm
levels.
Page 8-6

Instruction Manual
Touch the Quick Purge button on the functions

screen to deactivate purging once the desired levels
have been attained.
Page 8-7
Chapter 9
System Operation 9.2 Circulation Mode....9-1

9.3 Analyzers....9-3
9.4 Large Antechamber Operation....9-4
9.5 Mini Antechamber Operation....9-12
9.6 Freezers....9-15
9.7 Regeneration....9-17

Instruction Manual
Chapter 9: System Operation
CHAPTER 9: SYSTEM OPERATION

After the system has been fully commissioned, it may now be used for normal operation in
circulation mode. Prior to using the glovebox in circulation mode the box pressure parameters
can be adjusted for the upper and lower working limits. There are no recommended set
parameters, the setpoints are based on user preference.
At any time during use of the box, the foot pedal can be used to add or remove working gas
from the box temporarily. This is especially useful when attempting to place hands inside the
gloves and into the box when working in the positive pressure state.
Adjusting the parameters is done in the same manner as was done during the static leak test
and purging during the commissioning of the box. Please refer to Chapter 8 of this manual for
instructions.
9.2 Circulation Mode

The circulation process can only be started if all other functions
(purge and regeneration) are inactive.
There are no recommended setpoints for the circulation mode.
Upper and lower working pressure limits are based on user
preference.
If the oxygen level in the system rises above 150ppm, deactivate
circulation and begin purging. Continue purging until the oxygen
level drops below 100ppm.

the start screen.

decimal intervals.
or
parameter to display the alphanumeric keypad.
Use the keypad to adjust the parameters in the
same manner as before.
Page 9-1

Instruction Manual
Touch the End button.
Touch the Circulation Purifier 1 button on the

functions screen.
The regeneration and quick purge (if existing)
buttons are blanked out as these functions are
unavailable during the purging process.
The glovebox will continue to circulate the working gas until the function is deactivated.
Page 9-2

Instruction Manual
9.3 Analyzers
General Information
This section applies only to systems that are equipped with moisture and/or oxygen analyzers.
Touch the Functions button on the Start screen.
Touch the Analyzer

analyzer(s).
button
to
activate
the
Follow the steps above to deactivate the analyzer(s).
Page 9-3

Instruction Manual
9.4 Large Antechamber Operation

Follow the steps below to perform a transfer of materials from room environment into the
glovebox using the large antechamber. If equipped, the large antechamber evacuation and refill
cycles can be operated in Automatic or Manual modes. After placing the material into the large
antechamber please refer to the corresponding directions below for moving the material into the
glovebox.
Placing Material in the Antechamber
Before opening the outside antechamber door,

verify:
The inner antechamber door is closed;
The antechamber is not under vacuum
(gauge (A below) should read zero).
A
Turn the handle on the outer antechamber door in
a counterclockwise direction to open it.
Carefully open the antechamber door by lifting it in

an upward direction.
Page 9-4

Instruction Manual
Let go of the door handle and allow the door to

rise completely.
Gently pull out the sliding tray.
Place the materials to be used on the tray and

slide the tray back into the antechamber.
Page 9-5

Instruction Manual
Gently push the sliding tray back into the

antechamber.
Close the outer door by pulling it down until

groove at the end of the arm rests on the spindle.
Place four fingers, two each on opposite spindles,

on the door handle and gently turn in a clockwise
motion until the door touches the chamber.
Tighten turn more.
Page 9-6

Instruction Manual
Automatic Mode
Touch the Antechamber icon on the Start

Screen to display the antechamber screen.
Touch the Parameters button to display the

Antechamber parameters screen.
Use the drop down menus and information fields

to specify the settings for the antechamber cycle.
Touch the Back button to return to the
Antechamber layout screen.
Touch the Evacuate/Start Autom. button to

initiate the automatic antechamber cycle.
Page 9-7

Instruction Manual
A message on the upper right hand corner of the

screen displays the active step number and
description of the cycle.
Touch Manual button any time during the automatic antechamber

cycle to abort the cycle.
Manual Mode
Touch the Antechamber icon on the Start

Screen to display the antechamber screen.
If Automatic Mode is displayed in the upper right

hand corner, touch the Manual button to switch
from Automatic to Manual mode operation.
Skip this step if Manual Mode is already
displayed.
Page 9-8

Instruction Manual

evacuate the antechamber of room atmosphere.
Wait for the gauge to settle at the negative

position and continue to evacuate for:
Non-porous Material: 5 minutes
Porous Material: 12 hours

deactivate the evacuation process.
Touch the Refill button to refill the antechamber

with box gas.
Page 9-9

Instruction Manual
Non-porous Material
Wait for the gauge to settle at the halfway
mark on the gauge.
Porous Material
Wait for the gauge to settle at zero (0).
Touch the Refill button to deactivate the refill

process.
Repeat this process as necessary according the following recommended guidelines:

Non-porous Material
Evacuation (3 total cycles)
2 Additional Cycles 5 minutes each
Refill (3 total cycles 2 intermediate, 1 final)
1 Intermediate Cycle refill chamber halfway
1 Final Cycle refill chamber to atmosphere (or zero on the gauge).
Porous Material
Evacuation
1 Cycle 12 hours
Refill
1 Cycle refill chamber to atmosphere (or zero on the gauge).
Transferring Material from the Antechamber into the Glovebox
Grasp the door handle with two fingers in a

counterclockwise motion.
Page 9-10

Instruction Manual
Carefully open the antechamber door by lifting it

in an upward direction and guiding it until it stops.
Gently pull out the tray and transfer the material

into the glovebox.
At this time, materials to be transferred out of the
box may be placed on the tray.
After all materials are transferred into the

glovebox, grasp the door handle and gently pull
the door down until it rests on the door stop.
Page 9-11

Instruction Manual
Grasp the door handle with two fingers and turn in

a clockwise motion. Tighten finger tight and then
an additional turn.
9.5 Mini Antechamber Operation

Operating the mini antechamber is done manually. Follow the steps below to perform a transfer
of materials from room environment into the glovebox using the large antechamber.
Before opening the outside antechamber door, verify:
The inner antechamber door is closed;
The hand valve (A) is in the closed position;
The antechamber is not under vacuum (gauge
(B) should read zero).
The picture to the right displays the correct hand valve
position and the reading on the gauge at the zero
position.
Turn the handle on the outer antechamber door in a

counterclockwise direction to open it.
Page 9-12

Instruction Manual
Remove the door.
Place the materials to be used on the tray and slide the tray back into the antechamber.
Replace the cover and lock into place by turning in a clockwise motion.
Evacuate the antechamber by turning the hand valve

to the Evacuate position.
Wait for the gauge to settle at the negative position

and continue to evacuate for:
Non-porous Material: 3 minutes
Porous Material: 12 hours
Refill the antechamber by turning the valve to the

Refill position.
Page 9-13

Instruction Manual
Non-porous Material
Wait for the gauge to settle at the halfway mark
on the gauge.
Porous Material
Wait for the gauge to settle at zero (0).
Repeat this process as necessary according the following recommended guidelines:
Non-porous Material
Evacuation (3 total cycles)
2 Additional Cycles 3 minutes each
Refill (3 total cycles 2 intermediate, 1 final)
1 Intermediate Cycle refill chamber halfway)
1 Final Cycle refill chamber to atmosphere (or zero on the gauge).
Porous Material
Evacuation
1 Cycle 12 hours
Refill
1 Cycle refill chamber to atmosphere (or zero on the gauge).
Turn the hand valve to the Closed position.
Turn the handle on the inner antechamber door in a

counterclockwise direction to open it.
Remove the door.
Page 9-14

Instruction Manual
Remove the materials to be used from the tray and slide the tray back into the antechamber.
Replace the cover and lock into place by turning in a clockwise motion.
Transferring Material out of the Glovebox

Follow the above steps in reverse to transfer materials out of the glovebox into room
environment.
9.6 Freezers
General Information
The actual temperature inside the freezer will always be displayed
even if the freezer is inactive.
This section applies only to systems that are equipped with freezers.
Touch the Common Parameters button on the

Start screen.
Touch the Freezer/Box Cooling button on the

parameters screen to display the Freezer/Box
Cooling parameters screen.
Page 9-15

Instruction Manual
Touch the number field next to the Setpoint

Temperature Freezer to display the alphanumeric
keypad. Use the keypad to adjust the parameters
in the same manner as before.
The default setpoint is -35C however, the freezer parameters can

be set at any temperature between -10C and -35C.

Touch the Functions button to display the
functions screen.
Touch the Freezer button to activate the freezer.
Page 9-16

Instruction Manual
9.7 Regeneration Mode

The regeneration process can only be started if the circulation
process is inactive.
Circulation Auto Start

If desired, the circulation process can be started automatically following the end of the
regeneration procedure. Follow the steps below to have circulation start automatically after the
regeneration procedure is completed. Skip this step to restart the circulation process manually
after the regeneration procedure is completed.
Touch the RKM (Purifier) icon on the start screen.
Touch the Parameter button on the purifier layout

screen.
Select Yes from the drop down menu.
Page 9-17

Instruction Manual
Regeneration
Follow the steps below to start the regeneration process.
Deactivate the circulation process, if active, by

touching the Circulation Purifier button.
Touch the Regeneration Purifier button on the

functions screen.
The circulation button is blanked out as this function
is unavailable during the purging process.
Page 9-18

Instruction Manual
The purifier layout screen is displayed with the

following message.
Please confirm that you wish to regenerate the
purifier?
This message must be acknowledged (yes or no)
before regeneration will initialize.
Using the regulator, turn on the regeneration gas supply and set the pressure to 5psi.
Place the end of the regeneration vent line running from the VRS valve on the valve block
located on the back of the purifier to a 100ml (minimum size) collection vessel.
Vent the line and vessel to fume hood or other facility ventilation system.
A second message is displayed:

Prove flow of the regeneration gas! If OK
Confirm this button
This message must also be acknowledged before
regeneration will initialize. Touch the message on
the screen after confirming the flow is correct
according to the above information.
After acknowledging the second message the

purifier layout screen can remain displayed. This
screen will display the active step in the
regeneration process.
or
Touch the End button to display the start screen. To
display the purifier layout screen at any time during
the regeneration process touch the RKM icon on the
start screen.
After completing a regeneration process, the vacuum pump oil
should be changed. Refer to vendor supplied manuals for
instructions on changing the oil.
Used oil should be disposed of in accordance with all facility,
local and federal guidelines.
Page 9-19
Chapter 10
Maintenance &
Troubleshooting
10.2 Maintenance Requirements....10-1

10.3 Touch Panel Maintenance....10-2
10.4 Replacing the Gloves....10-5
10.5 Moisture Analyzer Maintenance....10.6
10.6 Alarms....10-12

Instruction Manual
Chapter 10: Maintenance & Troubleshooting
CHAPTER 10: MAINTENANCE & TROUBLESHOOTING

This chapter provides general information for general maintenance of a single purifier glovebox
system and troubleshooting various problems which may be encountered during operation of
the system. For information regarding troubleshooting of vendor manufactured components,
please consult the vendor supplied user manuals.
Contact the M.Braun Service Department with questions
regarding any of the troubleshooting steps listed below.
10.2 Maintenance Requirements

Daily Maintenance and Service
Windows:
Clean the exterior with a mild solution of soap or detergent and lukewarm
water.
Using a soft lint free cloth or sponge, gently wash the sheet to loosen dirt and
grime and rinse well with clean water.
To prevent water spotting, thoroughly dry with chamois or cellulose sponge.
Avoid the use of abrasive cleaners, squeegees and/or other cleaning
implements that may mar or gouge the coating.
Compatible Cleaning Agents:
Aqueous Solutions of Soaps and Detergents
Windex1
Top Job2
Joy2
2
3
Mr. Clean
Fantastik
Formula 4094
1 Registered Trademark of Drackett Products Co.
2 Registered Trademark of Procter & Gamble
3 Registered Trademark of Texize, Division of Norton Norwich Products Inc.
4 Registered Trademark of Clorox Co.
Organic Solvents
Butyl Cellosolve Hexcel, F.O. 554
Kerosene NelecoPlacer
Naphtha (VM&P) grade Turco 5042
Alcohols
Methanol and Isopropyl Alcohol
Gloves:
Check all gloves for damage.

The surface of the gloves in which the users hands are placed should be
coated lightly with talcum powder to prevent drying and brittleness.
Replace gloves immediately if any signs of damage or wear are detected.
Never attempt to repair gloves.
Antechamber:
Check antechamber door seals for damage and/or debris. Coat lightly, as
needed, with high vacuum grease.
If the antechamber doors are difficult to open or close, apply a light coating of
white lithium grease or other lubricant to the pivot points and/or door spindle.
Connections:
Check all connections to ensure they are seated properly and are leak free.
Components:
Observe the maintenance instructions of the third-party manufactured

components as directed in supplied manuals.
Page 10-1

Instruction Manual
Quarterly and Annual Maintenance and Service

Quarterly
Inspect the gloves and glove ports, replace
as needed.
Inspect the fluorescent box lights, replace as
needed.
Inspect the solenoid valves, replace as
needed.
Leakage test.
Test of the functions.
Inspect box filters, replace as needed.
Annually
Replace the gloves.
Inspect the glove ports, replace as needed.
Inspect the fluorescent box lights, replace
as needed.
Inspect the solenoid valves, replace as
needed.
Leakage test.
Test of the functions.
Replace box filters.
Calibrate oxygen and moisture analyzers.
10.3 Touch Panel Maintenance

General Information
By accessing the System Screen the user can:
Calibrate the touch panel screen
Clean the touch panel screen
Adjust the date & time settings
Below are instructions for each function.
Screen Calibration
Touch the System button on the Start Screen to
access the System Settings Screen.
Touch the Touch Calibration button to activate

the calibration process.
Page 10-2

Instruction Manual
Five calibration crosses are displayed
succession at random points on the screen.
in
Follow the instructions displayed on the screen and

touch each calibration cross as it is displayed.
To accept the changes made during the calibration

process touch the screen at any time.
To reject the changes made during calibration wait
for 30 seconds until the overlaid timer bar has
reached zero. The system will revert back to the
default settings.
Upon completion of the calibration procedure,

touch the Main or Back buttons to access the Start
Screen and resume normal operation.
Screen Cleaning
Page 10-3

Instruction Manual
Touch the Screen Cleaning button.
All system functions are locked out for 30 seconds

while a screen with a timer bar is displayed to allow
the user to clean the screen without interfering with
system processes.
Clean the screen by applying a small amount of
non-abrasive cleanser directly to a soft lint free
cloth and gently wipe the screen. Do not apply the
cleanser directly to the screen!
Upon completion of the cleaning procedure, touch

the Main or Back buttons to access the Start
Screen and resume normal operation.
Setting the Date and Time

Page 10-4

Instruction Manual
Touch the input field for the date and/or time to
display an alphanumeric pad.
Enter the correct information according to the
instructions detailed at the beginning of Chapter 4.
After making the appropriate changes, touch the

Main or Back buttons to access the Start Screen
and resume normal operation.
10.4 Replacing the Gloves

General Information
M.Braun recommends replacing the gloves at regular intervals. The gloves must be changed
upon signs of wear and tear that may or have caused a leak.
Before replacing the gloves ensure that the glovebox is atmosphere
is safe to breathe. If necessary purge the glovebox to fill it with
ambient air prior to changing the gloves.
Carefully remove the two

securing the glove in place.
o-rings
Carefully remove the glove from the

gloveport.
Page 10-5

Instruction Manual
Slide the glove onto hand and insert arm
into the box through the gloveport
angling the thumb to a comfortable
working position.
Slide hand out of the glove and work the

cuff of the glove into the groove in the
gloveport closest to the glass. At this
time the thumb position can still be
adjusted by rotating the cuff of the glove.
Secure the glove with two o-rings.
Place the o-ring in the top of the
second groove from the glass and in
one smooth motion pull down across
the circumference of the gloveport.
Repeat the process for the remaining
glove(s) and o-ring(s).
After replacing gloves, purge the glovebox to remove any undesired
oxygen and/or moisture. (Refer to Chapter 8 Commissioning the
System, Section 8.4 Purging)
Gloveport Covers
M.Braun gloveport covers, which are used to assist in replacing gloves or to block off an unused
gloveport, are made for standard round glove ports and are available for either interior or
exterior use.
Internal gloveport covers allow the user to replace gloves without contaminating the glovebox
atmosphere with room air.
External gloveport covers allow the user to remove a glove(s) and continuing working within a
glovebox without contaminating the glovebox atmosphere with room air.
Contact the Service Department for further information regarding price and availability of the
gloveport covers.
Do not seal off all gloveports at the same time. Sealing off all
gloveports simultaneously could cause pressure to build up inside
the glovebox which could cause the window to crack or rupture.
10.5 Moisture Analyzer Maintenance

General Information
If it seems necessary to clean a moisture analyzer probe, follow the instructions carefully to
avoid damaging the sensor probe. Prior to cleaning the analyzer, contact the M.Braun Service
Department to determine if service by M.Braun is required or if the analyzer only requires
cleaning.
Page 10-6

Instruction Manual
Materials Required
Soft absorbent lint free cloth
Phosphoric Acid
Distilled Water
Personal Protective Equipment (gloves, goggles, apron)
Instructions
Touch the Functions button on the Start Screen.
Touch the Analyzers button to deactivate the

analyzers.
Remove the RJ45 plug connector from the

analyzer.
Page 10-7

Instruction Manual
Remove clamp and analyzer.
Place a blank KF40 cap over the pipe and secure

in place with the clamp.
Separate the protective cover on the probe by

turning in a counter clockwise direction.
Slowly and carefully remove the cover from the
probe.
Page 10-8

Instruction Manual
Place an absorbent lint free cloth under the probe
and moisten the probe with distilled water.
Wrap the moistened cloth around the probe. Gently

and slowly spin the cloth around the probe to clean
it.
Dry the probe in the same manner with a dry lint
free cloth.
Do not clean the probe by moving the cloth in

an up and down motion. This will damage the
coil and will render the analyzer unusable
resulting in the need for repair by M.Braun.
Place an absorbent lint free cloth under the probe

and moisten the probe with Phosphoric Acid.
Page 10-9

Instruction Manual
Carefully replace the protective cover and secure in
place by turning a clockwise direction.
Remove KF40 clamp and blank cap.
Carefully place the sensor end of the analyzer into

the port on the circulation piping.
Secure in place with the KF40 clamp.
Page 10-10

Instruction Manual
Insert the analyzer cable plug into the hole on the
analyzer by pushing the plug into the hole gently
until it snaps into place.
Touch the Analyzers button on the Functions

screen to reactivate the analyzers.
Page 10-11

Instruction Manual
10.6 Alarms
General Information
The following troubleshooting guidelines address the possible alarm warnings that the user may
encounter during operation of the system.
Contact MBRAUN directly with any questions that arise while
attempting to troubleshoot the system or if an alarm message is
displayed that is not included below. Do not attempt to resolve the
problem.
Circulation Gas Flow

There is no gas flow to the experiment during circulation process.
Check the gas supply line.
Is there gas from the main supply line into the
system?
Check the valve activation gas supply for
correct flow rate (between 50 and 80psi) and
adjust, if necessary.
Yes proceed to next step.

No turn on gas supply and continue normal
operation. Proceed to next step if there is still no
gas flow to the experiment.
Yes continue normal operation.
No Proceed to next step.
Did this correct the problem?

Check the connector plug on each VHA & VHE
valve to ensure it is securely tightened to the
valve body.
Yes continue normal operation

No contact M.Braun Service Department.
Did the valve open?
Regeneration Gas Flow

There is no gas flow through the system during regeneration process.
Check the gas supply line.
Is there gas from the main supply line into the
system?
Check the gas supply for correct flow rate
(between 5 and 10psi) and adjust, if necessary.
Yes proceed to next step.

No turn on gas supply and continue normal
operation. Proceed to next step if there is still no
gas through the system.
Yes continue normal operation.
No Proceed to next step.
Did this correct the problem?

Check the connector plug on each VRE and
VRS valve to ensure it is securely tightened to
the valve body.
Did the valve open?
Check each VRE and VRS valve on the active
filter for a yellow LED which indicates there is a
signal being sent to the valve from the PLC.
Is the light on?

No proceed to next step
Yes, the light is on but the valve is not open and

there is no flow to the experiment. contact
M.Braun Service Department.
Page 10-12

Instruction Manual
Power Supply
The Main Power switch on the front of the system is set to the On position but the system is not
functioning.
Is the system plugged into the main power Yes proceed to next step
supply?
No Turn the main power switch to the Off
position and plug the system in. Turn the
switch to the On position and resume normal
operation. Proceed to next step if this does
not fix the problem.
Open the electrical cabinet located inside the Did this correct the problem?
system. Check main power breakers F1. Yes continue normal operation
Reset the blown breaker.
If a breaker that has been reset blows again, contact the M.Braun
Service Department.
Purifier Inlet/Outlet Not Open

One or both of the valves controlling circulation flow to and from the purifier bed fails to open.
Possible reasons for failure are:
The gas pressure required to open the valve is too low;
The gas supply tanks are empty.
Reset the regulator on the gas supply to 80psi.
Did the valve open?

No contact the M.Braun Service Department.
Vacuum Pump VPG1: Motor Protective Switch Activated

The vacuum pump controlling antechamber evacuation is drawing too much current. Possible
reasons for failure are:
Debris in the vacuum pump;
Vacuum pump oil is low or degraded;
Vacuum pump is defective.
Check the vacuum pump oil level using the sight
glass. Top off the oil level as needed.

No proceed to the next step.
Did this fix the problem?

Check the vacuum pump oil color using the sight
glass, it should be clear. Change the oil in the
vacuum pump.

Open the electrical cabinet and check the F4
breaker. Reset it if it is blown.
Page 10-13

Instruction Manual
Blower GB1: Motor Protective Switch Activated

The blower has exceeded current draw limit. Possible reasons for failure are:
The filter(s) is clogged;
The blower is drawing too much amperage;
A power outage or power surge;
System restart;
The blower is defective.
Check the inlet and outlet filters inside the
glovebox. Replace or clean as needed.


breaker. Reset it if it is blown.

Deactivate circulation via the Functions menu.
Push the Fn button on the frequency converter
to reset it. Reactivate circulation.
Box Pressure Too Low

Vacuum Pump VPG1 Switched Off
The glovebox pressure has dropped to a level below -15mbar. Possible reasons for failure are:
The antechamber evacuation valve is open and the inner door is not sealed properly;
An open auxiliary vacuum source inside the glovebox not controlled by the PLC.
Turn the antechamber evacuation valves to the
closed position. Verify the inner door is sealed
completely.
Check auxiliary vacuum sources located within
the glovebox. Ensure all seals are seated
properly and securely. Fix the connections as
needed.


Box Pressure Too High

The glovebox pressure has risen to a level above +15mbar. Possible reasons for failure are:
Entering the glovebox, using the gloves, too quickly;
Upper working setpoint parameter is too close to the upper working limit;
Auxiliary gas supply is open.
Check the upper working setpoint parameter.
Adjust as necessary to a limit of 10mbar or
below.


Turn off any unused auxiliary gas supply.

Page 10-14

Instruction Manual
Sensor Box Pressure Defective

The main pressure sensor for the system has failed causing circulation, analyzers, gas in and
gas out to be deactivated. Contact the M.Braun Service Department to obtain a replacement
sensor.
O2 or H2O Sensor Defective

The oxygen or moisture analyzer is not displaying a numerical reading on the PLC screen.
Possible reasons for failure are:
The cable is damaged;
The RJ45 connector is not inserted correctly;
The sensor head is damaged or defective.
Check the RJ45 connector to ensure it is
plugged into the analyzer properly. Adjust as
necessary.


Unplug the RJ45 connector. Inspect the cable
and plug for damage. Reinsert the connector
back into the analyzer.

Freezer Motor Protective Switch Activated

Box Cooling Motor Protective Switch Activated
The compressor used to operate the freezer or box cooling unit has exceeded current draw
limit. Possible reasons for failure are:
The compressor is drawing too much amperage;
A power outage or power surge;
System restart;
The compressor is defective.
breaker (freezer) or F8 breaker (box cooling).
Reset it if it is blown.

Page 10-15

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Chapter 1 - General Information

Chapter 2 - Liability, Warranty and Safety

Edition January 2007

Operating Instructions Supplement A

B. Adams, Quality Manager, M.Braun, Inc.

Publication edited by means of:

Adobe Acrobat, Adobe Photoshop, Microsoft Word

This technical documentation is not liable to any

General Information 1.2 Contact Information....1-2

Operating Instructions Labmaster 130 and MB20G Systems

CHAPTER 1: GENERAL INFORMATION

MBraun, Inc. Edition 1/2007

Operating Instructions Labmaster 130 and MB20G Systems

1.2 Contact Information

MBraun, Inc. Edition 1/2007

2.1 General Information....2-1

Liability, Warranty 2.2 Liability....2-1

Operating Instructions Labmaster 130 and MB20G Systems

CHAPTER 2: LIABILITY, WARRANTY & SAFETY

MBraun, Inc. Edition 1/2007

Operating Instructions Labmaster 130 and MB20G Systems

2.4 Operation Guidelines

MBraun, Inc. Edition 1/2007

Operating Instructions Labmaster 130 and MB20G Systems

2.5 Safety General Hazards

2.6 Safety Mechanical

MBraun, Inc. Edition 1/2007

Operating Instructions Labmaster 130 and MB20G Systems

2.7 Safety Electrical

MBraun, Inc. Edition 1/2007

Operating Instructions Labmaster 130 and MB20G Systems

2.8 Safety Handling of Electronic Components

2.9 Safety Chemicals and Gases

Operating Instructions Labmaster 130 and MB20G Systems

2.10 Safety Symbols Used on the System

Pressurised gas hazard

Wear safety mask

Wear safety goggles

Wear protective gloves

The owner of the system is responsible to place adequate danger

2.11 Safety Emergencies

MBraun, Inc. Edition 1/2007

Operating Instructions Labmaster 130 and MB20G Systems

2.12 Additional Information

MBraun, Inc. Edition 1/2007

3.1 Description of the System....3-1

3.2 Pressure Control System....3-1

Operating Instructions Labmaster 130 and MB20G Systems

CHAPTER 3: PRINCIPLES OF OPERATION

3.2 Pressure Control System

Current pressure within the glovebox.

The range within the working setpoints at which the box

Upper Working Setpoint

Adjustable setpoint parameters used to establish the working

The fixed value range between which the working parameters

MBraun, Inc. Edition 1/2007

Operating Instructions Labmaster 130 and MB20G Systems

3.3 Purging the System

Performed on systems with no manual purge valve or automatic purge

Performed on systems with a manual purge valve located on the top of