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    Glucose 5min

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    bnkjaya
    This document provides information on the Enzopak glucose reagent kit for quantitatively estimating glucose in serum or plasma using the GOD/POD method. It has a low blank and end point formulation with a five minute incubation. The method is specific, accurate and linear up to 500 mg/dL. Glucose in the sample is oxidized by glucose oxidase to produce hydrogen peroxide which reacts with phenol and 4-aminoantipyrine in the presence of peroxidase to produce a red dye. The intensity of the color produced is directly proportional to the glucose concentration in the sample and is measured photometrically. Blood glucose levels are estimated to diagnose and monitor diabetes mellitus and other hormonal disorders

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Glucose 5min

    Uploaded by

    bnkjaya
    103 views2 pages
    This document provides information on the Enzopak glucose reagent kit for quantitatively estimating glucose in serum or plasma using the GOD/POD method. It has a low blank and end point formulation with a five minute incubation. The method is specific, accurate and linear up to 500 mg/dL. Glucose in the sample is oxidized by glucose oxidase to produce hydrogen peroxide which reacts with phenol and 4-aminoantipyrine in the presence of peroxidase to produce a red dye. The intensity of the color produced is directly proportional to the glucose concentration in the sample and is measured photometrically. Blood glucose levels are estimated to diagnose and monitor diabetes mellitus and other hormonal disorders

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    Glucose 5Min

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    This document provides information on the Enzopak glucose reagent kit for quantitatively estimating glucose in serum or plasma using the GOD/POD method. It has a low blank and end point formulation with a five minute incubation. The method is specific, accurate and linear up to 500 mg/dL. Glucose in the sample is oxidized by glucose oxidase to produce hydrogen peroxide which reacts with phenol and 4-aminoantipyrine in the presence of peroxidase to produce a red dye. The intensity of the color produced is directly proportional to the glucose concentration in the sample and is measured photometrically. Blood glucose levels are estimated to diagnose and monitor diabetes mellitus and other hormonal disorders

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    103 views2 pages

    Glucose 5min

    Uploaded by

    bnkjaya
    This document provides information on the Enzopak glucose reagent kit for quantitatively estimating glucose in serum or plasma using the GOD/POD method. It has a low blank and end point formulation with a five minute incubation. The method is specific, accurate and linear up to 500 mg/dL. Glucose in the sample is oxidized by glucose oxidase to produce hydrogen peroxide which reacts with phenol and 4-aminoantipyrine in the presence of peroxidase to produce a red dye. The intensity of the color produced is directly proportional to the glucose concentration in the sample and is measured photometrically. Blood glucose levels are estimated to diagnose and monitor diabetes mellitus and other hormonal disorders

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    ENZOPAK

    Last update 05-2010

    GLUCOSE (5 MIN.)
    (GOD/POD Method)
    Reagent kit for quantitative estimation of glucose in serum or plasma.
    1.
    2.
    3.
    4.

    Product Highlights :
    Low blank
    End Point formulation with five minutes incubation
    Suitable pack size as per customer need.
    Linearity & Accuracy as per international standard

    BACKGROUND AND SYNOPSIS :


    Conventional methods like ferricyanide, Nelson-Somogyii, Folin-Wu,
    O-Toludine involved lengthy procedures and were cumbersome. Some
    of these methods involved steps like precipitation, filteration and boiling.
    These methods have drawbacks, are non-specific, imprecise, insensitive
    and inaccurate.
    ENZOPAK Glucose is based on GOD/POD method (Glucose oxidase /
    Peroxidase) as described by Trinder and is specific and accurate. The
    method selectively estimates -D-Glucose in serum or plasma.
    PRINCIPLE :
    Glucose oxidase oxidises the specific substrate, -D-glucose, to
    gluconic acid and hydrogen peroxide is generated. Hydrogen-peroxide
    thus produced is acted upon by peroxidase and oxygen is liberated.
    The liberated oxygen is transferred to chromogen system consisting of
    4-aminoantipyrine and phenolic compound to produce red quinoneimine
    dye. The intensity of colour is directly proportional to the concentration
    of glucose and is measured photometrically at 505 nm (500-540 nm or
    with GREEN filter).
    Glucose Oxidase

    H2O2 + Phenolic Compound


    +

    4-Aminoantipyrine

    Gluconate + H2O2

    Glucose + O2 + H2O

    Peroxidase Coloured Complex

    +
    H2O

    An ISO 9001:2008, ISO 13485: 2003 & GMP


    Certified Company

    DIAGNOSTIC SIGNIFICANCE :
    Blood glucose estimations are generally carried out for the diagnosis
    and follow up of diabetes mellitus.
    Glucose circulating in blood plasma is in dynamic equilibrium with
    absorption of carbohydrates from intestine. High levels of Glucose induces
    glycogen synthesis and low levels generate carbohydrate, in turn, glucose.
    Similar pathways are also encountered with Fatty acids and Aminoacids
    (protein). Deficiency of insulin-like activity is a condition resulting in
    hypoglycemia. Hyperglycemia and Hypoglycemia are also associated
    with various hormonal disorders e.g. Pituitary, Thyroid etc.
    PRESENTATION :
    No. of Bottles
    Pack Size
    5x100 ml. 4x250 ml 4x500 ml
    1 Glucose (Enzyme/Chromogen) 5
    4
    4
    2 Glucose (Phenol)
    1
    4
    4
    Standard (100 mg./dL)
    1
    1
    2
    Reconstitution Bottle
    1
    * 2 Glucose Phenol (Ready to use) is provided seperately.

    PRECAUCTION :
    ENZOPAK Glucose is for IN VITRO diagnostic use only.
    Reagent Contains Sodium Azide. DO NOT INGEST.
    PREPARATION OF WORKING REAGENT FOR
    5 X 100 ml. :
    Transfer the contents (powder mixture of Enzyme/Chromogen) of one
    bottle of 1 Glucose to the bottle provided for reconstitution. To this add
    100 ml. of 2 Glucose Phenol "Ready to Use". Mix well to dissolve and
    use as given in the procedure. Store at 2-80 C when not in use.
    4 X 250 ml. :
    Transfer the content (powder mixture of Enzyme/Chromogen) of one
    bottle of 1 Glucose to the bottle of 2 Glucose Phenol "Ready to Use".
    Mix well to dissolve and use as given the procedure. Store at 2-80C
    when not in use.
    4 X 500 ml. :
    Transfer the contents (powder mixture of Enzyme/Chromogen) of 1
    pouch/bottle of 1 Glucose to the bottle of 2 Glucose Phenol "Ready to
    Use". Mix well to dissolve and use as given the procedure. Store at
    2-80C when not in use..
    REAGENT STORAGE AND STABILITY :
    1 GLUCOSE
    2-80C
    2 GLUCOSE (Phenol)
    R.T.
    GLUCOSE STD. (100 mg/dL) 2-80C
    Working Reagent
    2-80C
    (Protected from light)

    Until Expiry
    Until Expiry
    Until Expiry
    3 Months

    SPECIMEN COLLECTION :
    Blood sample collected with any one of the anticoagulants like flouride,
    oxalate, EDTA, heparin or without any of the anticoagulants can be
    used. As soon as the sample is collected, seperate serum or plasma to
    prevent glycolysis.
    REACTION PARAMETERS : (END POINT)

    Type of Reaction
    : End Point

    Wavelength
    : 505 nm, (500-540 nm)

    Flowcell Temperature
    : 37 0C

    Incubation
    : 5 min. at 37 0C

    Std. Concentration
    : 100 mg/dL

    Sample Volume
    : 10 Microlitres (0.01 ml)

    Working Reagent Volume : 1.0 ml

    Zero setting with


    : Reagent Blank

    Light Path
    : 1.0 cm.
    PROCEDURE :
    For instruments with 1 ml. cuvette capacityPIPETTE INTO TEST TUBES BLANK STANDARD TEST
    WORKING REAGENT (ml)
    1.0
    1.0
    1.0
    STANDARD
    (ml)
    0.01
    SAMPLE
    (ml)
    0.01
    Mix well and incubate for 5 Min. at 370 C. Mix & read absorbance of
    standard and test at 505 nm (500-540 nm) or with GREEN filter against
    reagent blank.

    ENZOPAK

    GLUCOSE (5 MIN.)

    For instruments with 3 ml. cuvette capacity PIPETTE INTO TEST TUBES BLANK STANDARD
    WORKING REAGENT(ml)
    1.5
    1.5
    STANDARD

    (ml)

    SAMPLE

    (ml)

    TEST
    1.5

    0.02

    0.02

    Mix well and allow to stand for 5 Min. at 37 C. Then add....


    0

    DISTILLED WATER (ml)

    1.5

    1.5

    1.5

    Mix. & read absorbance of standard and test at 505 nm


    (500-540 nm or with GREEN filter) against reagent blank.
    STABILITY OF FINAL REACTION MIXTURE :
    The colour of reaction mixture is stable for 2 hours at room temperature,
    when protected from direct light.
    TEST RESULTS :
    Glucose Concentration (mg/dL) = Absorbance of Test
    X 100
    Absorbance of Std.
    REACTION PARAMETERS (FIX TIME)
    Type of Reaction
    : Fix Time/Two Point/Initial Rate
    Wavelength
    : 505 nm (500-540)
    Flowcell Temperature
    : 370C.
    Delay time
    : 30 seconds.
    Interval time
    : 60 seconds.
    Measuring time
    : 90 seconds
    No. of Readings
    : 2.
    Standard/Sample Valume : 10l(0.01ml)
    Standard Concentration : 100mg/dl
    Reagent Valume
    : 1.0 ml
    Zero Setting with
    : Distilled Water
    Light Path
    : 1.0 cm.

    LIMITATIONS FOR INTERFERENCE : As per studies carried out for


    interference. Following results were
    obtained.
    No Interference from Hemoglobin upto 187.5 mg/dl.
    No Interference from free Bilirubin upto 25.0 mg/dl.
    No Interference from Lipemic (Measured as Triglycerides) upto
    1000 mg/dl.
    NORMAL VALUES :
    Fasting : 70-110 mg / dL (3.90-6.11 mmol / lit)
    Two Hours Post prandial : upto 140 mg/dL (7.78 mmol/lit)
    LINEARITY :
    This method is linear upto 500 mg/dL. For sample value above 500
    mg/dL, dilute the sample suitably with 0.9 % saline & repeat the assay.
    Apply correction due to dilution to arrive at a final result.
    REFERENCES :

    TRINDER P , Annual Clinical Biochem 6, 24-25 (1969)

    HENRY. R. J. CANNON D.C., WINKELMAN I. W. Clinical


    Chemistry, Principles and Techniques 2nd edition. Harper
    & Row Publiser Inc. , N. Y., P-1288 (1974).

    TIETZ, N. W. (ed.) Fundamentals of Clinical Chemistry, 2nd


    edition. W. B. Saunders Co., Toronto, (1982).

    PROCEDURE :
    PIPETTE INTO TEST TUBES
    WORKING REAGENT(ml)

    STANDARD
    (ST)
    1.0

    STANDARD

    (ml)

    0.01

    SAMPLE

    (ml)

    TEST
    (TS)
    1.0
    0.01

    An ISO 9001:2008, ISO 13485: 2003 & GMP


    Certified Company

    Mix and aspirate. Record the absorbance of standard (ST) and Test
    (TS) at 30 seconds (ST1, TS1) and again at 90 seconds (ST2, TS2) at
    505 nm, against distilled water.
    TEST RESULTS :
    (TS2- TS1)
    Glueose Concentration =
    x100
    (ST2- ST1)
    (mg/dl)
    To convert mg/dL to mmol / lit. use the following factor 1 mmol / lit. = 18 mg/dL
    1 mg/dl. = 0.056 mmol/lit

    3/7, Industrial Estate, Gorwa, Vadodara 390 016 (INDIA)


    Web: www.reckondiagnostics.com
    Phone: +912652281631
    Email: mail@reckondiagnostics.com

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