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    Gel Electrophoresis Lab Questions

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    Scarlett Ramos
    This document summarizes the results of a genetics lab experiment analyzing an "Alu" insert. It includes: 1) The student was unable to determine their own genotype due to a failed DNA replication. 2) Genotypic frequencies for the student's class were 0% for +/+, 7% for +/-, and 93% for -/-. 3) Allele frequencies in the class were 3% for P and 97% for Q. 4) A USA-wide study found genotypic frequencies of 24% for +/+, 55% for +/-, and 21% for -/-. Allele frequencies were 52% for P and 48% for Q. 5) The student's class

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    Gel Electrophoresis Lab Questions

    Uploaded by

    Scarlett Ramos
    249 views4 pages
    This document summarizes the results of a genetics lab experiment analyzing an "Alu" insert. It includes: 1) The student was unable to determine their own genotype due to a failed DNA replication. 2) Genotypic frequencies for the student's class were 0% for +/+, 7% for +/-, and 93% for -/-. 3) Allele frequencies in the class were 3% for P and 97% for Q. 4) A USA-wide study found genotypic frequencies of 24% for +/+, 55% for +/-, and 21% for -/-. Allele frequencies were 52% for P and 48% for Q. 5) The student's class

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    life 120

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    This document summarizes the results of a genetics lab experiment analyzing an "Alu" insert. It includes: 1) The student was unable to determine their own genotype due to a failed DNA replication. 2) Genotypic frequencies for the student's class were 0% for +/+, 7% for +/-, and 93% for -/-. 3) Allele frequencies in the class were 3% for P and 97% for Q. 4) A USA-wide study found genotypic frequencies of 24% for +/+, 55% for +/-, and 21% for -/-. Allele frequencies were 52% for P and 48% for Q. 5) The student's class

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    249 views4 pages

    Gel Electrophoresis Lab Questions

    Uploaded by

    Scarlett Ramos
    This document summarizes the results of a genetics lab experiment analyzing an "Alu" insert. It includes: 1) The student was unable to determine their own genotype due to a failed DNA replication. 2) Genotypic frequencies for the student's class were 0% for +/+, 7% for +/-, and 93% for -/-. 3) Allele frequencies in the class were 3% for P and 97% for Q. 4) A USA-wide study found genotypic frequencies of 24% for +/+, 55% for +/-, and 21% for -/-. Allele frequencies were 52% for P and 48% for Q. 5) The student's class

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    Analysis and Interpretation of Results Focus

    Questions
    By: Scarlett Ramos
    Life 120L
    March 13,2016.

    1. What is your genotype for the Alu insert in your PV92 region?

    Scarlett: I could not determine the genotype for the Alu insert in my PV92 because the DNA did
    not replicate properly. Meaning that it was not able to be seen in the picture after the current was
    passed through the agarose.

    2. What are the genotypic frequencies of +/+, +/, and / in your class population? Fill in
    the table below with your class data.

    Table 1. Observed Genotypic Frequencies for the Class


    Category

    Number

    Frequency (# of

    Homozygous (+/+)
    Heterozygous (+/)

    0
    1
    14

    0
    .07
    .93

    Homozygous (/)
    Total =

    = 1.00

    3. Make sure that these equations all line up they arent aligned on my copy
    What is the frequency of each allele in your class sample? Fill in the table below with your class
    data. Remember, a class of 32 students (N) will have a total of 64 (2N) instances of each locus.

    P=

    (2*0+1)/(2*15) = .033 ; approx. = .03

    Q=

    (2*14+1)/(2*15) = .9667 ; approx.= .97

    4. The following table presents data from a USA-wide random population study.
    Table 3. Genotypic Frequencies for Alu in a USA Sample

    Category
    Homozygous (+/+)
    Heterozygous (+/)
    Homozygous (/)

    Number
    2,422
    5,528
    2,050
    Total = 10,000

    Frequency
    0.24
    0.55
    0.21
    = 1.00

    Now, using the data above, calculate the allelic frequencies for the USA data as you did for
    your class population in Table 2.
    P= (2*2422+5528)/(2*10,000) = .5186= .52
    Q= (2*2050+5528)/(2*10,000) = .48=.48
    5. How do your actual class data for genotypic and allelic frequencies compare with those of
    the random sampling of the USA population? Would you expect them to match? What
    reasons can you think of to explain the differences or similarities?
    Our class data for the genotypic and allelic frequencies was very different from the USA
    population. Our class P frequency was .03 while our frequency for Q was .97. The USA
    population example had P value as .52 while Q was .48. I expected it have variation, but as much

    as my results showed me. What I could probably assume happened here was that most in our
    class room originate from a similar location and that would lead to a higher amount of people
    have similar allele frequency, leaving little amount of the other allele frequency. The USA survey
    probably involved many different locations giving it a high probability of different allele
    frequency explaining the high variety of allele frequency giving a more balance allele frequency
    results.
    6. Using the values for p and q that you calculated in Table 2 for your class population,
    calculate p2, 2pq, and q2. Do they come out to be the same as the genotype frequencies that
    you found in Table 1? If they do, your class resembles a Hardy-Weinberg genetic
    equilibrium. If your observed (actual) genotype frequencies are not the same as the
    expected values, what might be some of the reason(s) for the difference?
    P^2 = 9*10^-4
    2pq= .0438
    q^2= .5329
    My results are not the same as the expected values. It is probably due to misinterpreting the
    alleles correctly or error in calculation.
    Using the values for p and q that you calculated in Table 4 for the USA population sample,
    calculate p2, 2pq, and q2. Do they come out to be the same as the genotype frequencies that
    you found in Table 3? Does this USA-wide sample suggest that the population of the USA is in
    Hardy-Weinberg equilibrium?
    P^2= .2704
    2pq= .4992
    q^2= .2304
    They are similar to the results found in table 3. The USA- wide sample does suggest that the
    population of the USA is in the Hardy-Weinberg equilibrium.

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