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    Banana DNA Extraction

    Uploaded by

    Angelica P. Cabardo
    This document provides instructions for extracting DNA from a banana. The key materials needed are a half banana, distilled water, plastic bags and cups, shampoo, salt, isopropyl alcohol, a spoon, coffee filter, and a narrow tube. The instructions involve mashing the banana in water, mixing a soap solution, combining the banana mixture with the soap, filtering it, slowly adding alcohol to form DNA precipitate layers, and collecting the precipitated DNA. The process works by the soap breaking cell membranes to release DNA, salt enabling DNA strands to aggregate, and alcohol separating DNA from other cell components.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Banana DNA Extraction

    Uploaded by

    Angelica P. Cabardo
    110 views1 page
    This document provides instructions for extracting DNA from a banana. The key materials needed are a half banana, distilled water, plastic bags and cups, shampoo, salt, isopropyl alcohol, a spoon, coffee filter, and a narrow tube. The instructions involve mashing the banana in water, mixing a soap solution, combining the banana mixture with the soap, filtering it, slowly adding alcohol to form DNA precipitate layers, and collecting the precipitated DNA. The process works by the soap breaking cell membranes to release DNA, salt enabling DNA strands to aggregate, and alcohol separating DNA from other cell components.

    Original Description:

    Biology Laboratory

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    This document provides instructions for extracting DNA from a banana. The key materials needed are a half banana, distilled water, plastic bags and cups, shampoo, salt, isopropyl alcohol, a spoon, coffee filter, and a narrow tube. The instructions involve mashing the banana in water, mixing a soap solution, combining the banana mixture with the soap, filtering it, slowly adding alcohol to form DNA precipitate layers, and collecting the precipitated DNA. The process works by the soap breaking cell membranes to release DNA, salt enabling DNA strands to aggregate, and alcohol separating DNA from other cell components.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    110 views1 page

    Banana DNA Extraction

    Uploaded by

    Angelica P. Cabardo
    This document provides instructions for extracting DNA from a banana. The key materials needed are a half banana, distilled water, plastic bags and cups, shampoo, salt, isopropyl alcohol, a spoon, coffee filter, and a narrow tube. The instructions involve mashing the banana in water, mixing a soap solution, combining the banana mixture with the soap, filtering it, slowly adding alcohol to form DNA precipitate layers, and collecting the precipitated DNA. The process works by the soap breaking cell membranes to release DNA, salt enabling DNA strands to aggregate, and alcohol separating DNA from other cell components.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    Banana DNA Extraction

    What Do I Need?

    peeled banana
    distilled water
    plastic sealable bag
    plastic cup
    shampoo
    table salt
    chilled isopropyl alcohol (90%)
    spoon
    coffee filter
    clear narrow tube or jar (such as a spice jar)
    Optional: bamboo skewer & capped tube

    What Do I Do?
    1.
    2.

    3.
    4.

    5.

    6.
    7.

    First you will need to put the banana and c


    distilled water in the plastic bag, seal the bag and
    mash them to make your slurry.
    In the plastic cup, mix a solution of 1 tsp. shampoo,
    2 pinches of salt and 4 tsp. distilled water. Stir this
    solution slowly for about a minute until the
    shampoo dissolves in the water.
    Now you will add 2 tsp. of the banana slurry to the
    soap solution and stir for five minutes.
    Next, make a well with the coffee filter and place it
    in the empty cup. Do not let the filter touch the
    bottom of the cup. Pour in the liquid mixture and
    let it filter.
    Fill the narrow tube with 2 tsp. of cold isopropyl
    alcohol. Very slowly, add 2 tsp. of the filtered
    banana mixture so that there are two layers of
    liquid.
    Let the tube sit for 2-3 minutes without disturbing
    the solution. You will see the clear/white DNA
    precipitate into the alcohol layer.
    If you wish to keep the DNA, remove it using a
    bamboo skewer (a twirling motion works best) and
    place it in a capped tube filled with alcohol.

    Whats Going On?

    DNA is found in the cells of every living organism. It is


    incredibly small, but we can see it by extracting DNA
    and isolating chains of it.
    The soap solution contains sodium laurel sulfate, which
    can break up fats and proteins. During the DNA
    extraction, the soap pulls apart the fats (lipids) and
    proteins that make up the membranes surrounding the
    cell and nucleus. Once these membranes are broken
    apart, the DNA is released from the cell. The salt
    enables the DNA strands to come together, or
    aggregate.
    The DNA precipitates out of the solution when the
    alcohol is added. The alcohol separates the DNA from
    the other cell components, which are left behind in the
    water solution.

    Reuben H. Fleet Science Center P.O. Box 33303 San Diego, CA 92163 (619) 238-1233

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