ENGLISH

and

Strictly physiologic!
PROTOCOL

Please read carefully!

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Summary
THE EQUIPMENT.......................................................................................................................p.1

DUO Centrifuge and the BoX......................................................................................................p.1

The instruments.....................................................................................................................p. 2 4
Tubes and Blood collectors........................................................................................................p.4

A-PRF...........................................................................................................................................................p.5
Settings................................................................................................................................................p.5
Blood drawing...........................................................................................................................p.5 6
PRF clots removal...........................................................................................................................p.7

Preparation of the membranes with the BoX....................................................................p.8

Preparation of the membranes without the BoX.............................................................p.9
The PRF used as membranes...................................................................................................p.9

i-PRF...........................................................................................................................................................p.10
Settings.............................................................................................................................................p.10
Schema.............................................................................................................................................p.10
Applications.....................................................................................................................................p.11

OTHER SETTINGS..................................................................................................................p.12

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THE EQUIPMENT

DUO Centrifuge:

The new centrifuge DUO has a switch button that affords you to select
various settings mode for the A-PRF and i-PRF. The values of these
settings are preset and locked except for PRP / PRF / CGF (you can set
other values) . Simply turn the knob on the desired mode of operation, the
corresponding LED will light on. The machine is ready. Simply press the
button
to start the centrifugation.

PRF BoX :

PRF Box allows to get membranes always hydrated and of constant

thickness, but also to recover the exsudate rich in proteins: Vitronectin
and Fibronectin. You can also produce plugs of PRF.

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The instruments :

PRF Scissors
Allows to separate easily the fibrin clot from
the red cells and to cut membranes into
small pieces.

PRF Mini Tray

Used to prepare membranes and too:
- Separate the fibrin clot from the red blood cells
- Cut the membranes
- Fold the membranes
- Make punches

PRF Forceps
Is used to remove and manipulate
membranes.

PRF Bowl
Is used to mix the membranes cut into small pieces
with biomaterials. It can also be used for the cutting:
Put the membrane into the bowl and cut it with PRF
scissors.

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The instruments :
2

In the Sinus: this plate is used to

retrieve the PRF membrane (always)
folded twice and slide it into the
sinus to the desired location easily.
Introduce the pointed end of the pad
1 in the sinus window and drag the
PRF membrane on the
Schneiderian membrane.
Same process for a
collagen membrane.

d
a
P

Is used to position the PRF

membranes easily, anywhere,
using the pad as a cake server.
Drag the PRF membrane on Pad 2
and place it on the desired surgical
area. Same process for a collagen
membrane...

F
R
P

ll
a
m

Allows to compact and tamp

the bone filling materials in the
recipient sites (alveoli, sinus,
goshawks implants).

S
r
o

t
c
a

p
m
o

ig
B
or
t
ac
p
m
o
C

Allows to compact and tamp

the bone filling materials in the
recipient sites (alveoli, sinus,
goshawks implants).

n
o
o

u
o
D

bl

p
S
e

Allows to collect and handle

the bone filling materials in the
recipient sites (alveoli, sinus,
goshawks implants).

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The instruments :

Tourniquet

Tube-holder
Is used to hold the tubes and let them rest after the blood drawing

It must be tightened to the patients forearm

10 cm above the site of venipuncture.

Sticker
Is used to maintain in place the collector during the blood drawing

Tubes and Blood Collectors :

Blood Collector
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: red tubes

Class IIa medical device.

Glass tubes without anticoagulant or additives

To become familiar with the technique, we advise you to do the first PRF procedure and blood drawing

on the practitioner himself.

Settings:

Turn the switch on the

left to position 1: A-PRF

13

Let the tubes rest for 5 minutes

1. Press the ON/OFF button: on position ON at the back of the machine

2. The lid opens automatically
3. Take off the rubber
4. Close the lid
5. Turn the switch on the left to position 1: A-PRF, the red LED lights on.
Your machine is ready for use. The settings are preset and locked.
Details : 13 x 100 rpm / 8 minutes
6. To start the spin, press the START button
7. At the end of the spin, the lid opens automatically
! Caution! Starting the spin without removing the rubber may destroy the motor.

Blood Drawing:
Who can draw the blood? Surgeons, Dentists, Nurses, Doctors, Lab technicians

One single rule: The blood drawing has to be as fast as possible.

Its essential for the PRF clot quality.
Without anticoagulant in the tube, the blood starts to coagulate after 1 or 2min. Over this time, the blood
coagulates progressively and it becomes difficult to separate the elements .

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! Warning: For patients on anticoagulants:

- Dont change the settings
- At the end of centrifugation, let the tubes rest, they will coagulate with
a small delay.

Its not recommended to do the blood aspiration with a syringe!

tubes are under vaccum. The blood stops being collected when the tube is full.
The
When the tube is full, place it in the centrifuge.
of tubes collected: at least 2, max 12, always BALANCED 2 by 2.
Number
You MUST equilibrate the tubes in the centrifuge (opposite, 2 by 2).
If you do not respect this statement, youll get important vibrations during the centrifugation.
A few examples of balance:

a tube filled with water. If you havent collected an even number of tubes, this tube will be used
Prepare
to equilibrate others tube in the centrifuge.
see the protocol to maintain the vein permeable
If you want to draw
button.
Close the lid. Press on
Check that the speed displays It means that the rotor has started.
mutiple punction)

more than 12 tubes,

(see the video

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If you want to collect 8,10 or 12 tubes:

1. Start immediately the centrifugation of the first 4 or 6 tubes.
2. Start the collection of the 4 or 6 following tubes. Stop the machine while the 7, 9 or 11th tube are filling.
3. The rotor will stop at the same time of the eighth or the twelfth full blood filling.
4. Put the last 4 or 6 tubes and restart the spin.
The 4 or 6 first tubes will be centrifuged 1min more than the last ones. It doesnt change the clots quality . However, if
you collect 12 tubes once, the first tubes will be partially coagulated before you start centrifugation.

If you want to collect 10 tubes, youll draw 6 and 4... or 4 and 6.

PRF Clots removal:

Remove the tube caps before placing the tubes in the sterile tube holder and let them rest around 5 minutes.

Take the fibrin clot into the opened tube with the sterile PRF forceps.

To separate easily the fibrin clot from the red cells: Put the clot on the mini-tray covered with a gauze and use the
closed scissors to peel off the red clot.

Put the PRF clots on the BoX grid and cover

them with the tray, then put the lid on (always).

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Preparation of membranes in the BoX:

Why the BoX ?

- To get membranes of constant thickness and always hydrated
- To remain them intact for 2 or 3 hours (no dehydratation)
- To recover the exsudate (in the BoX): rich in proteins: Vitronectin and Fibronectin
- To produce plugs of PRF, for socket extraction filling ( in the white cylinders, with the piston)

With the PRF BoX you can:

Make membranes : Put the clots after separation on the grid. Cover with the tray and then put the lid. The
membranes will be ready for use after 2 min. The membranes remain intact.( without any dehydratation and with
a constant thickness...)
The exsudate collected in the bottom is very rich in proteins (Fibronectin and Vitronectin).

Make plugs of PRF: Place a clot in each cylinder and push it with the metal piston. The piston musnt be
partially or fully depressed (photo 1 and 2). It must be stopped at the edge of the white Teflon cylinder (photo 3).
The plugs can be used immediately for socket extractions filling.

NO

NO

YES

Keep the exsudate recovered at the bottom of the box:

- To hydrate Biomaterials
- To flush the surgical sites, sockets and cysts.
- To preserve the autogenous bone blocks (rather than in saline)

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Preparation of membranes without the BoX:

1.If you want to use the membranes immediately: cover the PRF clots after removal with a sterile
gauze and crush them with your thumb. Let the fibrin rest for 5 minutes. Otherwise it will retract and you will
get a smaller size membrane. If you wait for 5 minutes, the size of the membranes will be definitive.
2. If you use biomaterials: PRF membranes (1 or 2 membranes) are cut into small pieces in the bowl and
mixed with the granule.

The PRF used as membranes:

As a whole membrane : on the implants after being punched, on bone graft, under the gingiva, on the
palate: covering the harvest area of the connective tissue (membrane will then be sutured), etc...
Membrane(s) cut into small pieces: with the scissors supplied. These fragments will be mixed with
biomaterials.
In the sinus-lift: apply one or two PRF membranes folded twice under the Schneiderian membrane with
the PRF Pad. Mix 1 to 2 membranes cut into small pieces with the biomaterial.
Use 1 or 2 membranes for a lateral closure of the sinus.

If the sinus membrane is punctured, it can be repaired using 1 or 2 PRF membranes folded twice.
PRF immediately sticks to the Schneiderian membrane.

In the extraction sites, it is preferable to use the cylinders prepared in the PRF BoX. To compress the PRF
plugs in the socket, use a gauze hydrated and not a metal instrument.

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: orange tubes

10

Class IIa medical device.

Tubes without anticoagulant or additives

To become familiar with the technique, we advise you to do the first PRF procedure and blood drawing

on the practitioner himself.

Settings:

At the end of the centrifugation, an orange color

supernatant is formed on the surface

1. Use the i-PRF9 tubes (orange).

2. Turn the switch on position 3: i-PRF, the orange LED lights on.
The machine is ready for the use of i-PRF. The settings are preset and locked.
Details: 7 x 100 rpm / 3 minutes for the i-PRF.
To start centrifugation, press START/STOP button
At the end of centrifugation the lid will open automatically

3. At the end of the spin, an orange supernatant will form on the surface.
4. Penetrate the cap with a 21G needle (green) mounted on a syringe.
5. Place the bevel of the needle in the middle of the i-PRF supernatant, against the wall of the tube (better visibility).
6. Aspirate until the level of the red blood cells raises up to the needle bevel.
7. Remove the needle maintaining suction.
8. i-PRF remains liquid for about 10 -12 minutes, then it will clot. The injection will have to be done before the end
of these 10-12 minutes.

Schema :

Use a syringe with a

green needle

End of centrifugation

Penetrate the
stopper with the
needle inclined
so that the
bevel is close to
the tube wall

Aspirate and
collect the
supernatant

Remove the needle

maintaining suction

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Applications:

11

1. The blood for i-PRF must be drawed just before injection. It cannot be prepared in advance.
2. To make the drawing after the A-PRF, watch the video on our website www.a-prf.com called
multiple punctures. This video explains how to collect, simply and several times, with the same butterfly
needle, in the meantime of several minutes or hours.
3. The i-PRF can be injected:
a. Into the soft tissues
b. In the bone graft. Mix the granules with the A-PRF (as usual), and poor the i-PRF drop by drop to avoid an
overflow. Wait a few seconds and go on until you obtain the complete coagulation of the biomaterials (in less
than a minute). If you inject too fast, the i-PRF will overflow from the bone graft!
c. Into the sinus, after the filling . The granules will be fixed and coagulated.
d. The i-PRF can be used to coagulate the biomaterials before application. This is the steak technique: Use
the same technique of dropwise preparation.
e. Several videos are available on our website www.a-prf.com

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AUTRES RGLAGES :

12

speed
Turn the switch in the
middle on position 2:
PRP / PRF / CGF

lock

time

Press the ON/OFF button: on position ON at the back of the machine

2. The lid opens automatically
3. Take off the rubber
4. Close the lid
5. Turn the switch on position 2: PRP/PRF/ CGF, the green LED lights on.

Press the button

. When the program flashes, the settings can be changed. Turn the programming
button (fig 1.) on the right to increase the value and on the left to decrease it.
For setting the speed and time.
Press the button
. The speed in rev / min flashes. Set the speed with the button
The minimum value of the speed you can set is 500 rev / min.
(fig 1.)
Press the button
. The time value flashes.
Set the time with the button (fig 1.). You can adjust the centrifugation time from 30 seconds to 99 minutes.
Once the settings are completed, press the button
to start centrifugation.
To stop the centrifugation before the end of the program, press the button
. The hood will open
automatically once the rotor has stopped.
The settings remains in memory by default (after pressing START button).

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