Materials and Methods - Nata
Materials and Methods - Nata
Materials and Methods - Nata
Coconut water
Glacial acetic acid
Nata starter
Monobasic ammonium phosphate
Refined sugar
Rubber bands
Cheesecloth
Masking tape
Beakers
pH meter
Thermometer
Stirring rod
METHODS
sanitized breaker. 25 g of refined sugar was added to the coconut water together with 1.25
g of monobasic ammonium phosphate. The solution was stirred well and then the pH of
the solution was obtained. The mixture was heated until boiling and the temperature was
recorded. The solution was set aside to be cooled. The beaker was covered with a watch
glass. The temperature of the cooled solution was then acquired. 1.725 mL of glacial
acetic acid was added into the cooled solution. The glacial acetic acid was handled
carefully. With the use of the stirring rod, the solution was stirred well. The pH of the
starting solution was obtained. 31.25 mL of the nata starter was added to the solution and
it was stirred well. The beaker was covered securely with a cheesecloth and rubber band.
A thermometer was securely placed inside the beaker using a masking tape. The nata
culture was stored in a clean and dry place (25C) for about 15 days. The culture was
regularly checked and the changes/observations on the mixture were recorded. The
container was not agitated during the 15 days period. The nata de coco output was then