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    Sri Santika Fujianti
    Carbohydrates are polyhydroxy aldehydes and ketones or their derivatives that are made of 2 to 8 monosaccharides called oligosaccharides. A series of tests were conducted to identify different carbohydrates including the Molisch test, Benedict test, Barfoed test, Selivanoff test, and iodine starch reaction. The tests identified glucose, sucrose, maltose, arabinose, and amylum. Proteins are polymers of amino acids. Experiments aimed to identify free amino acids with the ninhydrin test and peptide bonds with the biuret test. Casein samples at different pH levels were tested to determine the isoelectric point between pH 3.8-4

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    Abstract

    Uploaded by

    Sri Santika Fujianti
    204 views2 pages
    Carbohydrates are polyhydroxy aldehydes and ketones or their derivatives that are made of 2 to 8 monosaccharides called oligosaccharides. A series of tests were conducted to identify different carbohydrates including the Molisch test, Benedict test, Barfoed test, Selivanoff test, and iodine starch reaction. The tests identified glucose, sucrose, maltose, arabinose, and amylum. Proteins are polymers of amino acids. Experiments aimed to identify free amino acids with the ninhydrin test and peptide bonds with the biuret test. Casein samples at different pH levels were tested to determine the isoelectric point between pH 3.8-4

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    Carbohydrates are polyhydroxy aldehydes and ketones or their derivatives that are made of 2 to 8 monosaccharides called oligosaccharides. A series of tests were conducted to identify different carbohydrates including the Molisch test, Benedict test, Barfoed test, Selivanoff test, and iodine starch reaction. The tests identified glucose, sucrose, maltose, arabinose, and amylum. Proteins are polymers of amino acids. Experiments aimed to identify free amino acids with the ninhydrin test and peptide bonds with the biuret test. Casein samples at different pH levels were tested to determine the isoelectric point between pH 3.8-4

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    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    204 views2 pages

    Abstract

    Uploaded by

    Sri Santika Fujianti
    Carbohydrates are polyhydroxy aldehydes and ketones or their derivatives that are made of 2 to 8 monosaccharides called oligosaccharides. A series of tests were conducted to identify different carbohydrates including the Molisch test, Benedict test, Barfoed test, Selivanoff test, and iodine starch reaction. The tests identified glucose, sucrose, maltose, arabinose, and amylum. Proteins are polymers of amino acids. Experiments aimed to identify free amino acids with the ninhydrin test and peptide bonds with the biuret test. Casein samples at different pH levels were tested to determine the isoelectric point between pH 3.8-4

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    © All Rights Reserved
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    CARBOHYDRATE

    carbohydrates are polyhydroxy aldehydes and ketones or their derivatives. in addition


    carbohydrate jiga prepared by 2 (two) to 8 (eight) monosaccharides called oligosakarida. the
    objective was to identify carbohydrates in samples by milic assays, identify reducing sugars in
    carbohydrate compounds by benedict test, identify monosaccharides and disaccharides with
    barfoed test, identify carbohydrates of ketosan groups with seliwanoff test, identify
    carbohydrates with iodine starch reaction, and identify sucrose hydrolysis with reagent barfoed,
    seliwanoff, and benedict. mplisch test with glucose, sucrose, maltose, arabinose, cellulose and
    amylum positive samples by producing purple rings but cellulose does not produce rings because
    it is difficult to hydrolyze. benedict tests with glucose, fructose, maltose and galactose positive
    samples resulted in a red but brick red sediment for sucrose and starch because it lacked reducing
    sugars. barfoed test with gulkosa samples, fructose is the fastest sedimentary monosaccharide.
    sucrose, lactose, and maltose including disaccharides. positive seliwanoff test for sucrose,
    fructose and negative for glucose because it is not a class of sinners. hydrolysis of positive
    sucrose for all reagents. starch reaction with positive iodine in acidic and neutral conditions form
    a blue solution.

    keywords: carbohydrates, molisch test, benedict, barfoed, seliwanoff, sucrose hydrolysis

    PROTEIN

    proteins are complex organic compounds with high molecules that are polymer molecules of
    amino acid monomers. this experiment aims to identify free amino acids in proteins with the
    ninhydrin test, identify peptide bonds in proteins with biuret tests, and identify the isoelectric tth
    of proteins shown by the pH that make up most deposits. on the ninhydrin test in which the
    albumin sample when added with the ninhydrin reagent will result in a blue solution due to
    deamination and decarboxylation reactions. a biuret test in which albumin and urea samples were
    added 10% NAOH and 0.1% CuSO4 added will result in a purple solution due to the formation
    of complexes between Cu2 + central atoms with peptide bonds in an alkaline atmosphere. an
    isoelectric point is a state at a certain pH when a positive charge will have a negative charge on
    the protein molecule. Casein sample added acetate buffer pH 6, 5.3, 5, 4.1, and 3.8. which will
    result in the most sediment or turbidity being the result of the isoelectric point. obtained
    isoelectric point of pH range 3.8-4.1

    keywords: protein, ninhdrin, biuret, isoelectric point


    LIPID

    lipid is a biomolecule compound used as a source of energy and is a structural component of


    membrane constituents as well as pelindng vitamins or hormones. the simplest lipids and many
    fatty acids as the constituent units are triacylglycerols commonly called fat, neutral fats or
    triglycerides. Qualitative analysis of fat can be done by solubility test, acrolein test, and test
    lieberman-burchard. a solubility test is used to identify lipid solubility with marked stains on the
    filter paper. this filter paper serves as a medium for knowing the solubility of fat. principle used
    in solubility test that is like dissolve like and solubility difference. of the samples tested, the
    results of positive observations dissolved in the filter paper ie chloroform, hot alcohol, and cold
    alcohol. the acrolein test was used to identify glycerol in the sample. principally the
    dehydratation reaction of glycerol with anhydrous KHSO4 to form an unsaturated aldehyde
    compound ie acrolein. Positive results are characterized by a distinctive smell of rancid odor.
    from observations of glycerol and ollive oil positive samples containing glycerol. the lieberman-
    burchard test is used to identify cholesterol. positive results are characterized by a dark green-
    colored LARUTANN.

    Keywords: lipid, trigligliserol, solubility, acrolein, lieberman-burchard

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