Scribd Logo
Upload

Welcome to Scribd!

  • 224 views

    JECFA Beta Carotenes - (Vegetable) - INS-No.-160a (Ii) PDF

    Uploaded by

    Grisselda Priliacita
    This document defines and provides specifications for carotenes (vegetable). It states that carotenes (vegetable) are obtained from solvent extraction of carrots, palm fruit, sweet potato, and other edible plants, with the main coloring principles being alpha- and beta-carotenes. It establishes specifications for identity, purity, residual solvents, lead content, and method of assay.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    JECFA Beta Carotenes - (Vegetable) - INS-No.-160a (Ii) PDF

    Uploaded by

    Grisselda Priliacita
    224 views2 pages
    This document defines and provides specifications for carotenes (vegetable). It states that carotenes (vegetable) are obtained from solvent extraction of carrots, palm fruit, sweet potato, and other edible plants, with the main coloring principles being alpha- and beta-carotenes. It establishes specifications for identity, purity, residual solvents, lead content, and method of assay.

    Original Title

    JECFA beta Carotenes-(Vegetable)-INS-No.-160a(ii).pdf

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document defines and provides specifications for carotenes (vegetable). It states that carotenes (vegetable) are obtained from solvent extraction of carrots, palm fruit, sweet potato, and other edible plants, with the main coloring principles being alpha- and beta-carotenes. It establishes specifications for identity, purity, residual solvents, lead content, and method of assay.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    224 views2 pages

    JECFA Beta Carotenes - (Vegetable) - INS-No.-160a (Ii) PDF

    Uploaded by

    Grisselda Priliacita
    This document defines and provides specifications for carotenes (vegetable). It states that carotenes (vegetable) are obtained from solvent extraction of carrots, palm fruit, sweet potato, and other edible plants, with the main coloring principles being alpha- and beta-carotenes. It establishes specifications for identity, purity, residual solvents, lead content, and method of assay.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    You are on page 1of 2

    CAROTENES (Vegetable)

    Prepared at the 51st JECFA (1998), published in FNP 52 Add 6 (1998)


    superseding specifications prepared at the 44th JECFA (1995), published in
    FNP 52 Add 3 (1995). ADI "acceptable", provided the level of use does not
    exceed the level normally found in vegetables, established at the 41st JECFA
    in 1993. Metals and arsenic specifications revised at the 59th JECFA (2002).

    SYNONYMS Natural ß-carotene, carotenes-natural; CI Food Orange 5, mixed carotenes,


    INS No. 160a(ii); CI (1975) No. 75130; CI (1975) No. 40800 (ß-Carotene)

    DEFINITION Carotenes (vegetable) are obtained by solvent extraction of carrots (Daucus


    carota), oil of palm fruit (Elaeis guinensis), sweet potato (Ipomoea batatas)
    and other edible plants with subsequent purification. The main colouring
    principles are alpha- and ß-carotenes of which ß-carotenes account for the
    major part. Minor amounts of gamma- and delta-carotenes and other
    pigments may be present. Besides the colour pigments, this substance may
    contain oils, fats and waxes naturally occurring in the source material. The
    only solvents used for the extraction are acetone, methanol, ethanol, propan-
    2-ol, hexane, carbon dioxide and vegetable oils.

    The main articles of commerce are solutions or suspensions in food grade


    vegetable/plant oil. This is for ease of use and to improve stability as
    carotenes easily oxidise.

    Class Carotenoid

    C.A.S. number 7235-40-7

    Chemical formula C40H56 (ß-Carotene)

    Structural formula

    all-trans-ß-Carotene

    Formula weight 536.88 (ß-Carotene)

    Assay Content of carotenes (calculated as ß-carotene) is not less than declared

    DESCRIPTION Red-brown to brown or orange to dark orange solid or liquid

    FUNCTIONAL USES Colour

    CHARACTERISTICS
    IDENTIFICATION

    Solubility (Vol. 4) Insoluble in water

    Spectrophotometry A cyclohexane solution of the sample (1 in 200,000) shows maximum


    (Vol. 4) absorptions at 440-457 and 470-486 nm

    Colour reaction A spot of a solution of the sample in toluene (about 400 µg /ml of ß-carotene)
    on a filter paper turns blue 2-3 min after application of a spray or drop of 20%
    solution of antimony trichloride solution in toluene.

    PURITY

    Residual solvents Not more than 50 mg/kg, singly or in combination, of acetone, hexane,
    (Vol. 4) methanol, ethanol and propan-2-ol

    Lead (Vol. 4) Not more than 5 mg/kg


    Determine using an atomic absorption technique appropriate to the specified
    level. The selection of sample size and method of sample preparation may be
    based on the principles of the method described in Volume 4, “Instrumental
    Methods.”

    METHOD OF Proceed as directed under Colouring Matters, Total Content by


    ASSAY Spectrophotometry in Volume 4, using the following conditions:

    W (g) = amount of the sample to obtain adequate absorbance


    V1 = V2 = V3 = 100 ml
    v1 = v2 = 5 ml
    A1%1 cm = 2500
    lambda max = 440-457 nm

    You might also like