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    Expasy

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    Leticia Bonancio Cerqueira
    This document summarizes the results of analyzing a protein sequence from UniProtKB/Swiss-Prot for peptide masses after in silico digestion with Glu C endopeptidase. It lists 10 peptide masses between 500-3455 Daltons along with each peptide's position in the protein sequence. The protein analyzed was hemoglobin subunit alpha.

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    Expasy

    Uploaded by

    Leticia Bonancio Cerqueira
    28 views1 page
    This document summarizes the results of analyzing a protein sequence from UniProtKB/Swiss-Prot for peptide masses after in silico digestion with Glu C endopeptidase. It lists 10 peptide masses between 500-3455 Daltons along with each peptide's position in the protein sequence. The protein analyzed was hemoglobin subunit alpha.

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    digestion hemoglobin

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    expasy

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    This document summarizes the results of analyzing a protein sequence from UniProtKB/Swiss-Prot for peptide masses after in silico digestion with Glu C endopeptidase. It lists 10 peptide masses between 500-3455 Daltons along with each peptide's position in the protein sequence. The protein analyzed was hemoglobin subunit alpha.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    28 views1 page

    Expasy

    Uploaded by

    Leticia Bonancio Cerqueira
    This document summarizes the results of analyzing a protein sequence from UniProtKB/Swiss-Prot for peptide masses after in silico digestion with Glu C endopeptidase. It lists 10 peptide masses between 500-3455 Daltons along with each peptide's position in the protein sequence. The protein analyzed was hemoglobin subunit alpha.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    25/07/2018 ExPASy - PeptideMass

    Home | Contact
    PeptideMass

    PeptideMass
    The entered protein is: P69905

    The selected enzyme is: Glu C (phosphate)

    Maximum number of missed cleavages (MC): 0

    All cysteines in reduced form.

    Methionines have not been oxidized.

    Displaying peptides with a mass bigger than 500 Dalton.

    +
    Using monoisotopic masses of the occurring amino acid residues and giving peptide masses as [M+H] .

    You have selected HBA_HUMAN (P69905) from UniProtKB/Swiss-Prot:

    Hemoglobin subunit alpha (Alpha-globin) (Hemoglobin alpha chain)

    Chain Hemoglobin subunit alpha at positions 2 - 142 [Theoretical pI: 8.73 / Mw (average mass):
    15126.36 / Mw (monoisotopic mass): 15116.88]
    mass position #MC peptide sequence
    3455.9459 87-117 0 LHAHKLRVDPVNFKLLSHCL LVTLAAHLPAE
    2135.0419 32-48 0 RMFLSFPTTKTYFPHFD
    1723.9238 8-24 0 KTNVKAAWGKVGAHAGE
    1718.9296 49-65 0 LSHGSAQVKGHGKKVAD
    1699.9741 128-142 0 KFLASVSTVLTSKYR
    1057.5312 118-127 0 FTPAVHASLD
    1018.4873 77-86 0 MPNALSALSD
    1010.5265 66-75 0 ALTNAVAHVD
    601.3191 2-7 0 VLSPAD

    94.3% of sequence covered (you may modify the input parameters to display also peptides < 500
    Da or > 100000000000 Da):
    10 20 30 40 50 60
    VLSPADKTN VKAAWGKVGA HAGEygaeal eRMFLSFPTT KTYFPHFDLS HGSAQVKGHG

    70 80 90 100 110 120


    KKVADALTNA VAHVDdMPNA LSALSDLHAH KLRVDPVNFK LLSHCLLVTL AAHLPAEFTP

    130 140
    AVHASLDKFL ASVSTVLTSK YR

    Display the list of masses in raw text format to be exported into an external application

    SIB Swiss Institute of Bioinformatics | Disclaimer

    https://web.expasy.org/cgi-bin/peptide_mass/peptide-mass.pl 1/1

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